LOCUS       NZ_ARPF01000020        22794 bp    DNA     linear   CON 18-FEB-2024
DEFINITION  Streptomyces sp. SolWspMP-sol2th B083DRAFT_scaffold_17.18_C, whole
            genome shotgun sequence.
ACCESSION   NZ_ARPF01000020
VERSION     NZ_ARPF01000020.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. SolWspMP-sol2th
  ORGANISM  Streptomyces sp. SolWspMP-sol2th
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 113992)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Improved High-Quality Draft of Streptomyces sp. SolWspMP-sol2th
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 113992)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name :: Streptomyces sp. SolWspMP-sol2th
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 03:17:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,475
            CDSs (total)                      :: 6,400
            Genes (coding)                    :: 6,297
            CDSs (with protein)               :: 6,297
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 31 of 103
            Pseudo Genes (incomplete)         :: 82 of 103
            Pseudo Genes (internal stop)      :: 12 of 103
            Pseudo Genes (multiple problems)  :: 20 of 103
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:24:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 81004
            Orig. end                         :: 103798
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            ARPF01000020.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
FEATURES             Location/Qualifiers
     region          1..22794
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="2"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22794
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22794
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12794](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12794
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            complement(892..2280)
                     /locus_tag="B083_RS0128395"
     CDS             complement(892..2280)
                     /EC_number="2.4.1.15"
                     /GO_function="GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018101576.1"
                     /locus_tag="B083_RS0128395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="WP_028419552.1"
                     /transl_table=11
                     /translation="MVTEHAEATAQVLVASNRGPITYTLGEDGTLDAKRGGGGLVSGLS
                     AVDDKLWVCAALGEGDREAVRRGVGEPGVRMLDIDAEVHADAYNGIANSVLWFVHHLLY
                     QTPVEPVFDAEFRRQWASYETYNRAFAQALAEEAGEGAAVLVQDYHLALVPGMLRELRP
                     DLRIGHFSHTPWAPVDYFRLLPDDIGEQLLRGILGADRAAFLTRRWADAFIGCCTEILG
                     GTGRTRIGVHGLGADADFLRRRSREADVDERMAALREQVGEDRKTIVRVDRTELSKNIV
                     RGLHAYRALLETRPEWRERVVHVAFAYPSRQDLAVYRDYTAAVQSLATEINGTYGTESW
                     TPVVLHVKDDFARSLAAYRLADVALVNPIRDGMNLVAKEVPVVSDHGCALVLSREAGAY
                     EELGEDAIVVNPYDVAGTAEALHEALSMGTEERTGRTKRLAEAATALPPQRWFLDQLEA
                     LRQE"
     gene            complement(2487..3437)
                     /locus_tag="B083_RS0128400"
     CDS             complement(2487..3437)
                     /EC_number="2.4.1.266"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511020.1"
                     /locus_tag="B083_RS0128400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="WP_018489027.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.1e-11,
                     bitscore: 37.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLEEVERWLTRRSWSSADRPLDRLLDARTAEPHRTSVSVVLPALN
                     EEATVGAIVATIRRELMEKVRLVDELVVIDSGSTDATAAVARQAGARVVHRDAVLPRIP
                     ALPGKGEVLWRSLLVTSGEIVCFVDADLKDFSADFVSGTVGPLLTDPTVHFVKAMYDRP
                     FGDTAGQGGRVTELVARPLLNLHWPQLAGFVQPLGGEYAVRRSLLERLPFPVGYGVELG
                     LLVDALHTVGLDALGQVDVGVRRHRHQDGQALGRMAAAIYRTAQLRLSRGHLVRPALTQ
                     FERGAEGFVPRTHAVDTEERPPMREIAEYAERWAA"
     misc_feature    3398..3413
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.07"
                     /tool="antismash"
     misc_feature    3463..3483
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 22.36"
                     /tool="antismash"
     regulatory      3591..3718
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /regulatory_class="riboswitch"
     gene            3737..5032
                     /gene="thrC"
                     /locus_tag="B083_RS0128405"
     CDS             3737..5032
                     /EC_number="4.2.3.1"
                     /GO_function="GO:0004795 - threonine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thrC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010056010.1"
                     /locus_tag="B083_RS0128405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="threonine synthase"
                     /protein_id="WP_028419553.1"
                     /sec_met_domain="PALP (E-value: 1.4e-62, bitscore: 204.6,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVQTIAGNATTASVDLGPAVALSCRECGERFDLGPLFACASCFG
                     PLEVAYDLPAGDPEALRKRIEAGPDNIWRYAPLLPVPADVAGKPNLNPGFTKLVKADNL
                     ARELGVTGGLYVKDDSGNPTHSFKDRVVAIAVEAARAFGFTTLSCSSTGNLAGAVGAAA
                     ARAGLRSCVFIPHDLEQGKVVMAAVYGGELVGIEGNYDDVNRFCSELIGDPLGEGWGFV
                     NVNLRPYYGEGSKTLAYEICEQLGWRLPDQIVIPIASGSQLTKIDKGFQELIRLGLVED
                     RPYKIFGAQAEGCSPVSAAFKAGHDVVRPQKPNTIAKSLAIGNPADGPYVLDIARRTGG
                     AVEDVNDEQVVDAIKLLARTEGIFGETAGGVTVGVTKKLIEAGVIDPALTTVVLNTGDG
                     LKTLDAVSATSQATATIKPSLDAFRAAGLASA"
     gene            5086..5364
                     /locus_tag="B083_RS0128410"
     CDS             5086..5364
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007265553.1"
                     /locus_tag="B083_RS0128410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MoaD/ThiS family protein"
                     /protein_id="WP_028419554.1"
                     /transl_table=11
                     /translation="MSVKVRIPTILRTYTGGQAEVPAEGATLAEVIESLEKDHPGIAAR
                     VLDDQGKLRRFVNVYVNDDDVRFEGGLEAATPDGAGISIIPAVAGGC"
     gene            5774..5980
                     /locus_tag="B083_RS0128415"
     CDS             5774..5980
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1255: cold-shock
                     DNA-binding domain protein"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967346.1"
                     /locus_tag="B083_RS0128415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cold-shock protein"
                     /protein_id="WP_003967346.1"
                     /transl_table=11
                     /translation="MAQGTVKWFNAEKGYGFIAVDGGADVFVHYSAIQMDGYRTLEEGQ
                     RVEFEISQGQKGPQADMVKLAVG"
     misc_feature    complement(5822..5835)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     misc_feature    6253..6268
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.7"
                     /tool="antismash"
     misc_feature    complement(6253..6271)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.97"
                     /tool="antismash"
     misc_feature    6255..6269
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.7"
                     /tool="antismash"
     misc_feature    complement(6256..6271)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 21.7"
                     /tool="antismash"
     gene            6423..8045
                     /gene="groL"
                     /locus_tag="B083_RS0128420"
     CDS             6423..8045
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /gene="groL"
                     /gene_functions="other (smcogs) SMCOG1299: chaperonin
                     GroEL"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /locus_tag="B083_RS0128420"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES; misfolded
                     proteins enter the barrel where they are refolded when
                     GroES binds; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /product="chaperonin GroEL"
                     /protein_id="WP_003967345.1"
                     /transl_table=11
                     /translation="MAKIIAFDEEARRGLERGMNQLADAVKVTLGPKGRNVVLEKKWGA
                     PTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREGLR
                     NVAAGANPMALKRGIEKAVEAVSAALLEQAKDVETKEQIASTASISAADTEIGAKIAEA
                     MDKVGKEGVITVEESQTFGLELELTEGMRFDKGYISAYFATDMERMEASLDDPYILIVN
                     SKIGSVKDLIPLLEKVMQSGKPLLIIAEDVEGEALSTLVVNKIKGTFKSVAVKAPGFGD
                     RRKAMLADIAILTGGTVISEEVGLKLENAGLDLLGRARKVVITKDETTIVDGAGDSDQV
                     QGRVNQIRAEIENSDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKHRIEDAVR
                     NAKAAVEEGIVAGGGVALLQASAVFEKLDLTGDEATGANAVKLALEAPLKQIAVNGGLE
                     GGVVVEKVRNLPIGHGLNAATGEYVDMIAEGILDPAKVTRSALQNAASIAALFLTTEAV
                     IADKPEKAGAAAPGGMPGGDMDF"
     gene            8338..9042
                     /locus_tag="B083_RS01000000134195"
     CDS             8338..9042
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489030.1"
                     /locus_tag="B083_RS01000000134195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_028419555.1"
                     /transl_table=11
                     /translation="MTAMGNTGSGNTGSGNTELGNTGSGNTGSGNTGSGNTGSGKTELG
                     NTKSGNTRPGNTGGGPGRGERGRWSGRRLKGAALLLAGALLCAVGGLIVVTGTGLSKPA
                     GMRVETFGRIACHDTRAEKGQTVWHCFGETGAQQRANEAERERVARESLRVHVDGMPAS
                     ARIERTRITFADHDGRDDPETITATQVFDGGRWYAHSGQLVVYGMIPLLAGVGAAAWGV
                     YRVREAAGARGR"
     misc_feature    complement(9366..9381)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.15"
                     /tool="antismash"
     misc_feature    9367..9380
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.82"
                     /tool="antismash"
     misc_feature    9536..9551
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.05"
                     /tool="antismash"
     gene            9816..9950
                     /locus_tag="B083_RS1000000135975"
     CDS             9816..9950
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511015.1"
                     /locus_tag="B083_RS1000000135975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="keywimysin-related RiPP"
                     /protein_id="WP_018489031.1"
                     /transl_table=11
                     /translation="MKQQQEKAKKAYMKPSLFKQGDFSKKTAGYFYGSYKEYWVRRII"
     CDS_motif       9816..9899
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MKQQQEKAKKAYMKPSLFKQGDFSKKTA"
                     /locus_tag="B083_RS1000000135975_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       9900..9950
                     /prepeptide="core"
                     /locus_tag="B083_RS1000000135975_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="0.20"
                     /molecular_weight="2082.4"
                     /monoisotopic_mass="2081.0"
                     /core_sequence="GYFYGSYKEYWVRRII"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MKQQQEKAKKAYMKPSLFKQGDFSKKTA"
                     /leader_location="[9815:9899](+)"
     gene            10001..12073
                     /locus_tag="B083_RS0128435"
     CDS             10001..12073
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489032.1"
                     /locus_tag="B083_RS0128435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_032756546.1"
                     /sec_met_domain="Asn_synthase (E-value: 1.2e-34, bitscore:
                     113.3, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGLLRDYFVVLPDSVAGHTVAGQLPAPETTGAASDPTATGPAFA
                     PTAIGGTAGNATGTGIEAGTGPGIETGTSTSTATATSTATGTGDVVTVPHPSGRPWVLA
                     RPLFRKVSHLRRGRDALVLIGPGRVPEAVLADLLEGARDRAGLERRLARLPGLHHVIAH
                     LSGETWIRGTASGLRRIYHARHPQAGTIASDRPAVLAHLTGAPLDDGALALRLLDFLPH
                     PLSRRALWRGVHETGAGYGLALPVAAPGTAAAPGPHEYRWWEPPPPELPLAEGARRVGE
                     AIAASVRAHVGGLDRISCELSGGLDSTTLTFAARATAPAELSLLTVAARDRYSEDETWA
                     RRAAEAARGGPTGTPAPVHHIIPADQAPYFYADLAATSAELNDEPLPVAPGRARAHLLL
                     SRAHATGSGYHLTGYGGDELFLGLPHAYQDLFHGNPLTAWNHLSGLRHQLGWPLLPTLK
                     ALLNRSTFPRWLAGAVTTEPQPVTRTPLLSWGVRQSLRPWFTDHARTLITEEFRTAAER
                     AEPIDPWRGRHVDIDAVRMGARHFQAMEDIGMTIGLPVAAPFYDDRVLEATLAVRLPER
                     ISPWRYKPLLVEAARGVVPDALLARTTKDHMSSDEHQGLREHAPELADLWTGSRLADHG
                     LVDGRHLLRLAAEPFSPVLVEHSISSTVAGETWLRTAENAWPTHQLTPTTSEAVQ"
     aSDomain        12070..12315
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_B083_RS0128440_Stand_Alone_Lasso_RRE.
                     1"
                     /evalue="9.10E-28"
                     /identifier="RREFam002.1"
                     /label="B083_RS0128440"
                     /locus_tag="B083_RS0128440"
                     /protein_end="82"
                     /protein_start="0"
                     /score="87.1"
                     /tool="antismash"
                     /translation="MKLRKGIAVTTTEYGGVLLDEKDGSYWQLNDTSVIVVEALAAGQT
                     PEAAVDRIVAEFDVERAEAESDVAELTRQLVEAKILR"
     gene            12070..12321
                     /locus_tag="B083_RS0128440"
     CDS             12070..12321
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006126488.1"
                     /locus_tag="B083_RS0128440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_019762773.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 3.7e-27,
                     bitscore: 87.1, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 6e-16, bitscore: 51.4,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLRKGIAVTTTEYGGVLLDEKDGSYWQLNDTSVIVVEALAAGQT
                     PEAAVDRIVAEFDVERAEAESDVAELTRQLVEAKILRP"
     gene            12318..12794
                     /locus_tag="B083_RS0128445"
     CDS             12318..12794
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489034.1"
                     /locus_tag="B083_RS0128445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_028419557.1"
                     /sec_met_domain="PF13471 (E-value: 1.1e-26, bitscore: 86.0,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEMTMPRRGAGPGGVRLRAAIAAAFVLARFKPGRLRRVLTRCS
                     RGARPATYDETLEMYEAVTATSRRCAGRYGCLPRSVAIALACRMSGAWPDWCAGVRTAP
                     PFSPHAWVEAGGRTVGEQAEAADLRPLMVVTVREGVPGERGGGHDDGGGGGQEP"
     gene            12907..14838
                     /locus_tag="B083_RS0128450"
     CDS             12907..14838
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489035.1"
                     /locus_tag="B083_RS0128450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_232790482.1"
                     /transl_table=11
                     /translation="MAGATGATSTTGANGATGAAGTTGGTSTTSATSKAGTTGATGTAG
                     PTSETGATSITSTTGPSPLRLLLARVRPHRGVLVRAGLLSLAGSAAGLAMPLMAKYAVD
                     AFAADRSPVAPLIALTALVLAGACLSAYGRYLMARTGEGVVLRARDQLVGRIMRLKVPA
                     VDRLTPGDLQSRVTSDTTLLRTVLSSGLVESFNSVLMLLGTVAFMAYMDLTLLGVTLVV
                     IVGIGAVTALLMPRIQRAQLRAQESVGAMGAALDRVLQAFRTVKASGAEERETAAVARA
                     ARHAHDRGVSVAKWSSVSDVTMMMSIQLAFLAVLGVGGARVASGSLEISSLIAFLLYLF
                     YLMGPIGGLVEGWTGLQSGLAAVRRIDEVESLPGEPAEGSGGSKEGGGPLRVTFQDVTF
                     GYGDERAPAHKGVTFEVPAGGMVALVGPSGAGKSTVFSLLERFYDHDAGTIALDGRDIR
                     DWPLAELRASLAYVEQDAPVLAGTLRENLLFAAPDAPEERLTEAVTRTRLDALVARLPE
                     GLDTAVGHRGVTLSGGERQRIAIARALLRRPRLLLLDEVTSQLDAVNEQALRDVILELA
                     AQSTVLVIAHRLSTVRHADRIVVLEDGRVRTAGTHEELMAGDDLYRELATTQLAAAEVQ
                     EAQVQGAR"
     gene            complement(14843..15430)
                     /locus_tag="B083_RS0128455"
     CDS             complement(14843..15430)
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019762767.1"
                     /locus_tag="B083_RS0128455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Uma2 family endonuclease"
                     /protein_id="WP_028419559.1"
                     /transl_table=11
                     /translation="MTPSPADHAPRAQLSVEEFEQLARSAPETVTLELIDGKLEVKPVP
                     DGDHDEIIMWVAMQCMQQRPELRLYRERGLRIGGYRNGRARPDGALARAGRFAGDGEWS
                     EPDRILMTVEVTSHDHDTDSRDRVEKRDGYASAAIPVYLLVDRDDNTLVVHSEPEKGRY
                     RRQTTYAYGDTVHLPGPVDITLDSEELMNYTA"
     gene            complement(15487..16329)
                     /locus_tag="B083_RS0128460"
     CDS             complement(15487..16329)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489037.1"
                     /locus_tag="B083_RS0128460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_028419560.1"
                     /transl_table=11
                     /translation="MAKPGYGKRSAPGERPRTERDFAHLLPRDAAVAAYLDRLPDGSDI
                     SVKTLAKHLPYGQCALSTTLNRLQAGGHLRRGSECVVADDVMLWVTRTFFSRTAREDSW
                     WNAFTRGDASPDQPQRPTRSRAFILLAALGRTAPTLSLSSADCVRLEPRVSEWFARGAT
                     EAELLYALTAGLPSPVHCAARLVERRLTDKLPPERVPPGPGPRTVLRTLECGECGVPGS
                     PEALLGGLCRACRGPSPEPPAGPPGAVQPPGVRERMNAVRAGLVPRSERTYRFERTHR"
     misc_feature    complement(16108..16110)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(16509..16525)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.78"
                     /tool="antismash"
     misc_feature    complement(16676..16689)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.15"
                     /tool="antismash"
     misc_feature    complement(16732..16747)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.13"
                     /tool="antismash"
     gene            16828..17328
                     /locus_tag="B083_RS0128465"
     CDS             16828..17328
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489040.1"
                     /locus_tag="B083_RS0128465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="WP_028419561.1"
                     /transl_table=11
                     /translation="MTVEEFEEFYAQAVARLTGQLYVMTGDLQEAQDVVQEAFVKAWVR
                     RGRLERDGQPEAWIRTVAWRLAVSRWRFRRRSADAWGRGSGAPEHVAPPDPDHVVLVEA
                     LRGLPAQQRRTLTLHYLCDLTVEQIAGETGLSTSTIKTHLVRGRAALAHRLRDPRMEEA
                     PDV"
     misc_feature    complement(16843..16856)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.98"
                     /tool="antismash"
     gene            complement(17599..17814)
                     /locus_tag="B083_RS0128470"
     CDS             complement(17599..17814)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511006.1"
                     /locus_tag="B083_RS0128470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_078562693.1"
                     /transl_table=11
                     /translation="MNVDVDADVDVAVEVDVDEGVGASVVGMPPGTDDPGVYEPEGPVP
                     WAPGIVVLGVGGSVGVPAGGGGEGDG"
     gene            18052..19320
                     /locus_tag="B083_RS0128475"
     CDS             18052..19320
                     /GO_function="GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015610355.1"
                     /locus_tag="B083_RS0128475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="mannosyltransferase family protein"
                     /protein_id="WP_028419563.1"
                     /transl_table=11
                     /translation="MPILSPGLHPAEPRSDATSAPRANRPVKPVPYASEAPRRDRWASW
                     LTPADRRVLQLYLLTRLSLWTMAHGARWLFPRGSDVREAAPLLAPFQQWDANHYLHIAR
                     DGYFPAGAGPWLGDWDNREAFFPGYPLLLRAVHVVVPDWTAAGLLISLAAGAVAVLALS
                     RVARSYVPEDAAGHRTVLFFLLSPCTVFLAAGYTEALFLAFALPAWLAARRHQWAWACA
                     LTALATTVRVSGLFLAAAVAVLFVVTALQAGTGKAWRGVAWPALPGLPPLAYSWYLHAH
                     TGDWMAWKHAQERGWYREFHTPWEAWANTWNAAFDGVHTTGYAAMFQAELAAMLVGVAL
                     VALLARYGRWPEVVYVALSLWALGTSYWYTSIPRATLLWWPLWIGLAALSLRRPWLRTA
                     YLCVSAPLAALVALAFFTGRWAG"
     gene            complement(19416..19613)
                     /locus_tag="B083_RS0128480"
     CDS             complement(19416..19613)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018961743.1"
                     /locus_tag="B083_RS0128480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_032756553.1"
                     /transl_table=11
                     /translation="MPSRRADLTSVTWRKSSYSNSDGGNCVEVSDDFPGLIPVRDSKRP
                     EGPALVFGAPAWTAFVNDMR"
     gene            complement(19589..20437)
                     /locus_tag="B083_RS0128485"
     CDS             complement(19589..20437)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092770.1"
                     /locus_tag="B083_RS0128485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_028419565.1"
                     /transl_table=11
                     /translation="MPKRASDSGGEASARKMLHETLKRLRDRSGLSLEELHDETTYDRS
                     YLHKLETGARLGSLEVMAALDKVYGTGDQLQQLWELARDDAFGSRYQRFMQLEREATVR
                     YEYACSAIPGLLQTRAYATEVLEHSPPRDAAELQEDVDARMSRQSVLVGEDAPYFRAML
                     DEAVFRRAASQPAVWLEQLETLLEWQKRPNVTLQLLPFSAGLHDLMGGSLTVLWLANGK
                     AVAYLEGSKSGELVEDSGRVEELRLSYDLQRDKALSPEQTTEFIQQLVKEIRSCLPAEP
                     T"
     gene            20554..20823
                     /locus_tag="B083_RS0128490"
     CDS             20554..20823
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B083_RS0128490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157841120.1"
                     /transl_table=11
                     /translation="MSALPKYDPDCPRRAERIPDRPVMRVIDVQSQETYFDLPLMITFD
                     GSAQGPAYLRLEVDEVIDLVDELNFVLDVRVKGPVAQPCTRSGI"
     gene            21056..21382
                     /locus_tag="B083_RS01000000134200"
     CDS             21056..21382
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /locus_tag="B083_RS01000000134200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /protein_id="WP_050451724.1"
                     /transl_table=11
                     /translation="MNGALLTLWDLKLCIDVAALVLTELVTNAFQHGGGEDITVTWCAD
                     VTHLTMRVEDSSPVRPVLRAVDETSEGGRGLSIVEALADAWGVSEDGHAVHCALALAEY
                     AARA"
     repeat_region   21589..21861
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=102654..102682
                     /rpt_unit_seq="gggaccacccccgcacgcgcggggaccat"
     gene            complement(22082..22510)
                     /locus_tag="B083_RS0128500"
     CDS             complement(22082..22510)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377501.1"
                     /locus_tag="B083_RS0128500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_028419567.1"
                     /transl_table=11
                     /translation="MRFPSEARRDVQVRYTRPSCMGGFAWFTVDFEPLSDGRLGFEFVN
                     PLGPDDIGPECADAVREGILRWLTGATRNELTSHDRPPLPTAEELATGLHVRPEAGPGL
                     IALRAVLRHSRLHPVDSLPIAHLGAGWQAADKGVGRLW"
     misc_feature    22505..22517
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.9"
                     /tool="antismash"
ORIGIN
        1 catcgtcccc gaccccgagc aggcccgcgc ccaccccggc gccgccgagg cgctcgccgc
       61 gctcgccccg aaggtcgcct cgatcgccgt gatcaccggc cgcccggcag gcgtggccgt
      121 acggtacggc ggcttcgcgg gcgtccccgg cctggaccac ctcgtcgtcc tcggccacta
      181 cggcgccgag cgctgggatg ccgccaccgg caccgtgaac gctcccgccc cgcaccccgg
      241 cgtggccgag gcgcgggccg aactccccgg cgtactgcac gagttcggcg cctggcacgg
      301 cacctggatc gaggagaagg gccaggccgt cgccgtccac acccgccgtg ccgacgaccc
      361 gcaggccgtc ttcgagaccc tgcgcggccc cctcggcgag ctggccgccc gccacgggct
      421 gatcctcgaa ccgggccgcc aggtcctgga gttgcgcccg ccgggcatgg acaagggcgt
      481 cgcgctcgcg gagtacgtac gggaggtgga cgccacctcc gtgctgtacg cgggcgacga
      541 cctcggggac ctcgccgcct tcgccacggt ggagaagctg cgcggcgcgg gcacggaggg
      601 gatcccggga ctgctggtgt gcagcggcag cgccgaggtg cccgaactgg ccgagcgcgc
      661 cgacctgttg gtgccgggac cggccgccgt ggtggagctc ctggcggcgc tggccgaccg
      721 cctctgagcc gcgctcgcgc acacctggcc cgaccgcctc cgagccgtac cggaggtgcc
      781 ggaagtaccg ggagcgccgg cgcgcacgga gctgagcgcc gggagcgccg ggcgtacgga
      841 gctgagcgcc gggagcgccg ggagtgccgt gggcgccgtg agcggctccc ctcactcctg
      901 ccgcagcgcc tccagctgat ccaggaacca gcgctgcggc ggaagcgccg tcgccgcctc
      961 cgccagccgc ttcgtgcggc ccgtccgctc ctcggtgccc atcgacaggg cctcgtgcag
     1021 cgcctccgcc gtgcccgcca cgtcgtacgg gttcaccacg atcgcgtcct cgcccagttc
     1081 ctcgtacgcc cccgcctccc gtgacagcac cagcgcgcag ccgtggtcgg atacgacggg
     1141 gacctccttg gcgaccaggt tcatgccgtc gcggatcggg ttgaccaggg ccacgtccgc
     1201 cagccggtac gcggcgagcg agcgggcgaa gtcgtccttc acgtggagga cgacgggcgt
     1261 ccagctctcg gtcccgtacg tcccgttgat ctccgtggcc agcgactgca ccgccgccgt
     1321 gtagtcccgg tagaccgcga ggtcctggcg ggaggggtag gcgaaggcca cgtggaccac
     1381 gcgctcgcgc cactccgggc gggtctccag cagggcccgg taggcgtgca ggccgcgcac
     1441 gatgttcttg gacagctccg tgcggtccac ccgcacgatc gtcttccggt cctcgcccac
     1501 ctgctcccgc agcgcggcca tccgctcgtc cacgtccgcc tcgcgcgagc gccggcgcag
     1561 gaagtccgcg tccgccccca gcccgtgcac cccgatccgg gtccggcccg tcccgcccag
     1621 gatctccgtg cagcagccga tgaacgcgtc cgcccaccgg cgggtcagga acgcggcccg
     1681 gtccgcgccg aggatgcccc gcagcaactg ctcaccgatg tcgtcgggca gcagccggaa
     1741 gtagtccacc ggcgcccacg gggtgtgcga gaagtggccg atccgcaggt ccgggcgcag
     1801 ctcgcggagc atcccgggga cgagggccag gtggtagtcc tggaccagca ccgccgcccc
     1861 ttcccccgcc tcctccgcga gggcctgggc gaaggcccgg ttgtacgtct cgtaggaggc
     1921 ccactggcgc cggaactccg cgtcgaagac cggctccacg ggggtctggt acagcaggtg
     1981 gtggacgaac cacagcaccg agttggcgat gccgttgtac gcgtcggcgt gcacctcggc
     2041 gtcgatgtcc agcatccgta cgccgggctc accgaccccg cgccgcaccg cctcgcggtc
     2101 gccctccccg agggccgcgc agacccacag cttgtcgtcg acggcgctca gcccggagac
     2161 gagccccccg ccgccgcgct tggcgtcgag cgttccgtcc tcgccgagcg tgtacgtgat
     2221 ggggccgcgg ttggacgcga cgaggacctg ggcggtggct tcggcatgct cggtgaccat
     2281 gtagcggaac ctagcccgta agggaagccg ccaaacgcgc gtccggcgga ccggtcaccg
     2341 gcaccgcgcc acccaccgcg cccgcgcaca cctcacgccg gccgcctcgc ccgcgcccgc
     2401 ctcacgccgc ccaccgcgcc cgcgcacggc tcggggctgt cgaagctccg cgcccgcctc
     2461 ggagctgccg gagccccgcg cccgcctcac gccgcccacc gctccgcgta ctccgcgatc
     2521 tcccgcatgg gcggccgctc ctccgtgtcc accgcgtggg tgcgcggcac gaacccctcc
     2581 gccccccgct cgaactgggt cagcgcgggc cgcaccagat ggccccggga gagccgcagc
     2641 tgggccgtgc ggtagatcgc cgccgccatc cggcccagcg cctgcccgtc ctggtggcgg
     2701 tggcgccgca cccccacgtc gacctggccc agcgcgtcga ggcccacggt gtgcagcgcg
     2761 tcgaccagga gccccagctc cactccgtaa ccgaccggga acggcagccg ctccagcagc
     2821 gagcgccgta ccgcgtactc accgcccagc ggctgcacga acccggccag ctgcggccag
     2881 tgcaggttga gcagcgggcg ggccaccagc tcggtgacgc gaccgccctg acctgcggtg
     2941 tcgccgaacg ggcggtcgta catcgccttg acgaagtgca ccgtgggatc ggtgagcagc
     3001 gggccgaccg tccccgagac gaagtccgcc gagaagtcct tgaggtccgc gtcgacgaaa
     3061 cagacgatct ccccgctggt caccaggagc gaccgccaga gcacctcgcc cttgcccggc
     3121 agggccggta tccggggcag caccgcgtcc cggtggacca cccgcgcgcc cgcctggcgg
     3181 gcgacggcgg cggtcgcgtc ggtcgaaccg gagtcgatca ccaccagttc gtccaccagc
     3241 cggaccttct ccatcagctc ccgccggatc gtcgccacga tcgccccgac cgtggcctcc
     3301 tcgttcagcg cgggcagcac cacactcacc gaggtgcggt gcggttcggc ggtccgggcg
     3361 tcgaggagcc ggtccagcgg gcggtcggcg gacgaccagg accgcctggt cagccagcgt
     3421 tccacctctt cgagcacggt ctgaactccc tgtgtgtgat ccatctcgcc gttcggacga
     3481 ctatctcaac cgtcccgtcc ttcggttaca gtcttgaaca acgcggacga ccgtcgcatg
     3541 tcggggtccg tcgtcacaca aacgcccgga tcgaagatcc ggtgccacag agctcatcca
     3601 gagggactga gggaacggcc cgttgaagtc ccggcaaccc tcctgccgac cgcgaggtca
     3661 cggtggggaa ggtgccaatt ccgtcttgcg gcgaaatgcg ctgcgaggaa gatgaggaga
     3721 aagggcctcc gccatcatgg ctgttcagac catcgcaggt aatgccacca ccgcttccgt
     3781 ggacctcggt cccgccgtgg cgctttcctg ccgcgagtgc ggcgagcgtt tcgacctcgg
     3841 cccccttttc gcctgtgcgt cctgtttcgg gccgctggaa gtggcgtacg acctgcctgc
     3901 cggtgacccg gaggccctgc gcaagcgcat cgaggccggt cccgacaaca tctggcgcta
     3961 cgccccgctg ctgccggtcc ccgccgacgt ggccggcaag cccaacctga accccggctt
     4021 caccaagctg gtcaaggccg acaacctcgc ccgcgagctg ggcgtcaccg gcggtctgta
     4081 cgtcaaggac gactccggca acccgacgca ctccttcaag gaccgcgtcg tcgcgatcgc
     4141 cgtcgaggcc gcccgcgcct tcggtttcac caccctctcc tgctcctcga cgggcaacct
     4201 ggccggtgcc gtcggcgccg ccgccgcccg cgccggactg cgctcctgcg tcttcatccc
     4261 gcacgacctg gagcagggca aggtcgtcat ggccgcggtg tacggcggtg agctggtcgg
     4321 catcgagggc aactacgacg acgtgaaccg cttctgctcc gagctgatcg gggacccgct
     4381 cggcgagggc tggggcttcg tcaacgtcaa cctgcggccc tactacggcg agggctccaa
     4441 gaccctggcg tacgagatct gcgagcagct cggctggcgg ctgcccgacc agatcgtcat
     4501 cccgatcgcg tccggctccc agctgacgaa gatcgacaag gggttccagg agctgatcag
     4561 gctcggcctg gtcgaggacc ggccgtacaa gatcttcggc gcccaggccg agggctgctc
     4621 cccggtctcc gccgccttca aggccgggca cgacgtggtc cgcccccaga agccgaacac
     4681 catcgccaag tccctggcca tcggcaaccc ggcggacggc ccgtacgtcc tggacatcgc
     4741 ccgccgcacg ggcggcgcgg tggaggacgt gaacgacgag caggtcgtcg acgcgatcaa
     4801 gctgctggcg cgcaccgagg gcatcttcgg cgagacggcg ggcggtgtca cggtcggcgt
     4861 gacgaagaag ctgatcgagg ccggggtgat cgacccggcg ctcaccaccg tcgtcctgaa
     4921 caccggtgac gggctcaaga cgctcgacgc ggtctccgcg acctcgcagg cgacggccac
     4981 catcaagccg agcctggacg cgttccgcgc cgcgggcctc gcctccgcct gatcatcccg
     5041 atcgccccga tcggcccgac cgcagcagtc agaaaggcgt ccgccatgag cgtcaaggtc
     5101 cgcatcccca ccatcctccg cacctacacg ggcggccagg ccgaggtgcc ggccgagggc
     5161 gccacgctcg ccgaggtcat cgagtccctg gagaaggacc accccggcat cgccgcccgc
     5221 gtcctggacg accagggcaa gctgcgccgc ttcgtgaacg tgtacgtcaa cgacgacgac
     5281 gtgcgcttcg agggcggcct ggaagcggcc acgcccgacg gcgccggtat ctcgatcatc
     5341 ccggccgtgg ccggcggctg ctgaacccga acgaacacca ttgccccctc cgcccctcgg
     5401 ggaggagggg gcaattctgc atggttgagc acggtacagt tggggaaacc cccttcgctg
     5461 cacgcgccgc ccgcatatga gaatgcgccc ggacccgata agaagcagcc aaagtgcgtg
     5521 aaccccttga ggcataagtc acctttgcct ggcccgactt gcccgtaaat ctcagcaatc
     5581 ccgcatattc gggcgttcgg ccgtgcccgg aattctcgtc cgattgacct gttgcagagg
     5641 gcagttgggc ggatacattc ggccgcggtc gacgcgctgc ggcgctgccc accctctcct
     5701 gtcggagggt gagttccgac ccggggccgc gaagtgcggt cccgtgcaag ggccagttat
     5761 aggggagtta ggcatggctc agggcaccgt caagtggttc aacgcggaga aggggtacgg
     5821 cttcatcgcg gtcgacggtg gtgcggatgt tttcgtccac tacagcgcga tccagatgga
     5881 cgggtaccgc accctcgaag agggtcagcg agttgaattc gagatctcgc agggccagaa
     5941 ggggccccag gcagacatgg tcaagctcgc cgtcggctga gcccgacacg gccgtccgcc
     6001 gtcactactc acgcacgtag ggcccgaccc cggcatccgg ggatcgggcc ctacgcgcgt
     6061 gccgggaccg atacgtgcgt cccgggaccg atacgcgcgt gccgggaccg atccgcgggc
     6121 gcgcccggac cggaccccgg ggacgcccgg accggcctgc gaggacgccc ggaccggtcc
     6181 cggatcgggc ggggaccgac ccgggaccgc atggcgggct ctcgtcgaag gcgcttgcac
     6241 tctcgggggt cgagtgctaa tcattggcat tagcactctc caggtgagag tgacagaact
     6301 tggaccgggc cggtgaggcc cgcacgcgtg ttggggcaag gaaccaccac gcacgcaggc
     6361 cgtccgtcgc gggcgcctct cggtccggag caatccaccc ctgtccggga ggaccacttc
     6421 acatggccaa gatcatcgcg ttcgacgagg aggcacggcg cggtctcgag cgcgggatga
     6481 accagctcgc cgacgccgtc aaggtcaccc tcggccccaa gggccgtaac gtcgtcctcg
     6541 agaagaagtg gggcgcgccc acgatcacca acgatggtgt atccatcgcc aaggagatcg
     6601 agctcgagga cccgtacgag aagatcggtg cggagctggt caaggaggtc gccaagaaga
     6661 cggacgacgt cgccggcgac ggtacgacca ccgccaccgt cctcgcccag gctctcgtcc
     6721 gcgagggtct gcgcaacgtc gccgcgggcg ccaacccgat ggccctcaag cgcggtatcg
     6781 agaaggccgt cgaggccgtc tccgccgctc tgctggagca ggccaaggac gtggagacca
     6841 aggagcagat cgcttcgacc gcctccatct ccgccgccga caccgagatc ggcgcgaaga
     6901 tcgccgaggc gatggacaag gtcggcaagg aaggcgtcat caccgtcgag gagtcccaga
     6961 ccttcggtct ggagctggag ctcaccgagg gtatgcgctt cgacaagggc tacatctcgg
     7021 cctacttcgc gaccgacatg gagcgtatgg aggcgtcgct cgacgacccg tacatcctga
     7081 tcgtcaactc gaagatcggc agcgtcaagg acctcatccc gctgctggag aaggtcatgc
     7141 agtcgggcaa gccgctgctg atcatcgccg aggacgtcga gggcgaggcg ctctccaccc
     7201 tggtcgtcaa caagatcaag ggcacgttca agtccgtcgc cgtcaaggcc ccgggcttcg
     7261 gcgaccgccg caaggccatg ctcgcggaca tcgccatcct caccggtggc accgtgatct
     7321 ccgaggaggt cggcctcaag ctggagaacg ccggtctcga cctgctcggc cgcgcccgca
     7381 aggtcgtcat caccaaggac gagacgacca tcgtcgacgg tgccggtgac agcgaccagg
     7441 tccagggccg cgtcaaccag atccgcgccg agatcgagaa ctccgactcg gactacgacc
     7501 gcgagaagct ccaggagcgc ctcgcgaagc tggccggcgg cgtggccgtc atcaaggccg
     7561 gcgccgccac cgaggtggag ctcaaggagc gcaagcaccg catcgaggac gcggtgcgca
     7621 acgccaaggc cgccgtcgag gagggcatcg tcgccggtgg tggcgtggcc ctgctccagg
     7681 cctcggccgt cttcgagaag ctcgacctca cgggcgacga ggcgaccggc gccaacgccg
     7741 tgaagctcgc gctggaggcc ccgctcaagc agatcgccgt caacggtggt ctcgagggtg
     7801 gcgtcgtcgt ggagaaggtc cgcaacctgc cgatcggtca cggcctcaac gccgcgacgg
     7861 gtgagtacgt cgacatgatc gccgagggca ttctcgaccc ggcgaaggtc acgcgctccg
     7921 ccctgcagaa cgccgcctcc atcgccgcgc tcttcctcac caccgaggcc gtcatcgccg
     7981 acaagcccga gaaggccggc gcggccgccc cgggtggcat gccgggcggt gacatggact
     8041 tctgatcggt tccggtccgc cggtcgatcg aggttccgcg tacgacgggt gcgtcaggta
     8101 cgccgagtac gcaggtacgt cccacagggg cggcatctcc cagccgggag gcgccgcccc
     8161 tgtggcgtgt ccggggccgc gggggagtgc ggagccgggc cgtacgggaa ccgccggcgc
     8221 gtgggaacgg tccaacggtt catacgggca acggttcacg cgggcgacgg tggatacgcg
     8281 ggaacggctg acgcgcgcgg gggacgcgcg cggcacggga acgggggcgg ggcggtcatg
     8341 acggccatgg gcaacaccgg gtcggggaac accgggtcgg ggaacaccga gttggggaac
     8401 accgggtcgg gcaacaccgg ttcgggcaac accggttcgg gcaacaccgg gtcggggaaa
     8461 accgagttgg ggaacaccaa gtcgggcaac accaggccgg ggaacaccgg tggcgggccg
     8521 gggcgcgggg agcgcgggcg gtggagcggg cggcggctca agggcgccgc gctgctcctc
     8581 gcgggggcgc tcctgtgcgc ggtgggcggc ctcatcgtgg tcaccggcac gggcctctcg
     8641 aagccggccg gcatgcgggt ggagaccttc ggccggatcg cgtgccacga cacgcgcgcc
     8701 gagaaggggc agaccgtctg gcactgcttc ggggagaccg gggcccagca gcgcgccaac
     8761 gaggcggagc gcgagagggt cgcccgggag tccctgcgcg tccacgtcga cgggatgccc
     8821 gcgtcggcgc ggatcgagcg gacccggatc accttcgcgg accacgacgg ccgcgacgac
     8881 cccgagacga tcaccgcgac ccaggtcttc gacggcggcc ggtggtacgc gcactcgggc
     8941 cagctcgtcg tctacgggat gatccccctc ctcgcgggcg tcggagcggc ggcgtggggc
     9001 gtctaccggg tacgcgaggc ggcgggcgcg cggggccgct gagggccgct gcgaacgtct
     9061 tggcggacgc cccggcggat gccccggcgg actcagggcc ccctcatggg gcgcccaagg
     9121 cgcgcccggg gtgggcccga gggcgggccc ggggccgccc ggtcacagcg cactcgcggc
     9181 gtgcgcgcgg gggcgcgggc aggccgtcca cgaggtgcag gcggagttcg ggcggagttc
     9241 gggcggggct cgggcgggcg cagacgggct cggagggggc ctcggcgtgc ccgagcggtc
     9301 ccggacggga ccgaacgagc ggtcgggagt ggccgaatgg ctacttgccg tgcccggcac
     9361 cgtacggcaa cacacgttcg agcggtttcg tcgatgccgg tggcgcgccg accgtgcgtg
     9421 aggtagggcc ggtacgggcg ctgccaggcc tccgccccgc tggttcctcc gtccggccgc
     9481 gccccgcgcg atctcttggc tgctcactgc cggggtcggc ccgccgcccg tagttattgg
     9541 tccagtccat tgcgtcggac cggcccgccg ctgtgcgact gcacatgccg accggtcggt
     9601 gcctcggcgg tggtacgggc cgtcgcggcc gcgctctctc cgctcgcgct atccggccaa
     9661 cttcgcgcgg taagtcggaa tcacgcgtac cggtccggcg gcgggacatg atggccctgg
     9721 tgatcacctg gtgaacacca ggcccaccaa taactccagt tatgcgacca cctgttgggc
     9781 ataacggtcg tcaaccgcac agaagggaag gtgtcatgaa gcagcagcag gagaaggcca
     9841 aaaaggccta catgaagccg tcgctgttca agcagggcga cttttcgaag aagaccgccg
     9901 gttacttcta cggctcgtac aaggagtact gggtccggcg catcatctga accgccgacc
     9961 ggttccctgt gcacggtgtg agaacttccg aaaggtgaag atgcccggcc tgctgcgtga
    10021 ctacttcgtc gtcctgccgg acagcgtggc gggccacacg gtggccgggc agcttcccgc
    10081 accggagacg accggggcgg cgtcggaccc gaccgcgacc gggcccgcgt tcgccccgac
    10141 ggcgatcggc ggcaccgccg gcaacgcgac cgggaccggg atcgaggccg ggaccggccc
    10201 cgggatcgag accggcacca gcaccagcac cgcgaccgcg accagtaccg cgaccgggac
    10261 cggagacgtc gtgaccgtcc cgcatccctc cggccgcccc tgggtcctcg cgcgccccct
    10321 gttccgcaag gtcagccacc tccgccgagg ccgtgacgcc ctggtgctca tcggccccgg
    10381 ccgcgtcccc gaggccgtgc tcgccgacct cctggagggc gcccgcgacc gggccgggct
    10441 ggagcggcgc ctcgcccggc tccccggcct ccaccacgtc atcgcccacc tctccgggga
    10501 gacctggatc cggggcaccg cctccggact gcgccggatc taccacgccc ggcacccgca
    10561 ggccgggacc atcgcctcgg accgcccggc cgtcctggcc cacctgaccg gcgcccccct
    10621 ggacgacggc gcgctggccc tgcgcctgct cgacttcctg ccgcaccccc tgagccggcg
    10681 cgccctgtgg cggggcgtgc acgagaccgg ggccgggtac ggactggccc tgcccgtcgc
    10741 agcaccgggc acggccgccg cccccggccc tcacgagtac cgctggtggg agcccccgcc
    10801 gccggaactc cccttggcgg agggcgcccg cagggtcggg gaggccatcg ccgcctccgt
    10861 acgggcccat gtcggcggcc tggaccggat cagctgcgaa ctgtcgggcg gcctggactc
    10921 cacgaccctc accttcgccg cccgcgcgac cgccccggcg gagctgtcac tgctcaccgt
    10981 ggcggcccgg gaccgctaca gcgaggacga gacgtgggcg cgccgggcgg cggaggcggc
    11041 acggggcggg cccacgggca cccccgcccc ggtccaccac atcatccccg ccgaccaggc
    11101 cccgtacttc tacgcggacc tggccgccac ctcggccgag ctgaacgacg aaccgctccc
    11161 cgtcgccccc ggccgcgccc gcgcccacct gctcctgagc cgcgcccatg ccaccgggtc
    11221 gggctaccac ctcaccggct acggcggcga cgaactcttc ctcggcctgc cccacgccta
    11281 ccaggacctc ttccacggca accccctcac cgcctggaac catctcagcg gcctgcgcca
    11341 ccagctcggc tggcccctcc ttcccaccct gaaggccctg ctgaaccgct ccacgttccc
    11401 ccgctggctg gcgggcgcgg tcaccaccga accgcagccg gtcacccgga ccccgctgct
    11461 ctcctggggc gtacgccagt ccctgcgccc ctggttcacg gaccacgcgc gcaccctgat
    11521 caccgaggag ttccggaccg ccgccgagcg ggccgagccg atcgacccgt ggcgggggcg
    11581 gcacgtggac atcgacgcgg tacggatggg ggcgcggcac ttccaggcga tggaggacat
    11641 cggcatgacg atcggactgc cggtcgcggc ccccttctac gacgaccggg tcctggaggc
    11701 caccctcgcg gtacgcctcc cggagcggat cagcccctgg cgctacaagc cgctgctcgt
    11761 cgaggcggcg cgcggggtcg tgccggatgc gctgctggcc cgtacgacga aggaccacat
    11821 gtcctccgac gaacaccagg gcctacgcga acacgcgccg gaattggcgg acttgtggac
    11881 cggctcccgc ctcgccgacc acggactggt ggacggccgc cacctgctcc ggctggcggc
    11941 cgaacccttc tcgccggtcc tggtcgagca ctccatcagc tccaccgtgg ccggggagac
    12001 ctggctccgc acggcggaaa acgcctggcc cacccaccag ttgaccccca ccaccagcga
    12061 ggcagtgcag tgaagctcag aaaaggcatc gccgtcacga cgaccgagta cggcggagtc
    12121 ctgctcgacg agaaggacgg cagctactgg cagttgaacg acaccagcgt catcgtggtc
    12181 gaggctctcg ccgccgggca gacccccgag gcggccgtcg accggatcgt cgcggagttc
    12241 gacgtcgagc gggccgaggc ggagtcggac gtggccgagc tgacccgcca gctcgtcgag
    12301 gcgaagatcc tgcgcccatg accaccgaga tgaccatgcc ccgccggggc gcgggcccgg
    12361 gaggcgtgcg gctgcgggcc gccatcgccg ccgcgttcgt cctcgcccgc ttcaaaccgg
    12421 gccgcctgcg acgggtgttg acccggtgca gcaggggcgc ccgcccggcg acgtacgacg
    12481 agacgctgga gatgtacgaa gccgtcaccg ccaccagccg ccgctgcgcg ggccgttacg
    12541 gctgcctgcc ccgctcggtg gccatcgcgc tggcgtgccg catgtcgggg gcgtggcccg
    12601 actggtgcgc cggggtccgg acggcaccgc cgttctcccc gcatgcttgg gtggaggcgg
    12661 ggggccgcac ggtcggcgag caggcggagg cggccgatct gcgtcctctg atggtggtga
    12721 cggtacgcga gggggttccg ggtgagcgcg gtggcgggca cgacgacggg ggcgggggcg
    12781 gacaggagcc ctgaggggca gccgggaggg cgtggggagc ctcaggagcc gggaggggct
    12841 ggggcgcctg aggcgagcac accaccctcc ggagcgagcg acccgggcgc ccccggcccg
    12901 accggtatgg ccggcgcgac cggcgccacc agcacgaccg gcgcgaacgg cgctacgggt
    12961 gcggccggca cgaccggcgg caccagcacg accagcgcca ccagcaaggc cggcacgacc
    13021 ggcgccacag gcacggccgg cccgaccagc gagaccggcg ccaccagcat caccagcacg
    13081 accggcccct cccccctccg tctgctcctc gcccgggtcc gcccgcaccg gggcgtcctg
    13141 gtccgggcgg gcctgctctc gctcgccggg tcggcggccg ggctggcgat gccgctgatg
    13201 gcgaagtacg cggtcgacgc gttcgccgcc gaccgctcgc ccgtggcccc gctcatcgcc
    13261 ctcaccgcgc tcgtcctcgc gggcgcgtgc ctctccgcgt acgggcgtta cctcatggcc
    13321 cgtacgggag aaggcgtcgt cctccgtgca cgggaccaac tggtcggccg catcatgcgg
    13381 ttgaaggtcc cggcggtcga ccgtctcacc cccggcgacc tccagtcccg ggtgaccagc
    13441 gacacgaccc tcctccggac ggtcctctcc agcggtctgg tcgagtcgtt caacagcgtg
    13501 ctgatgctgc tggggacggt cgcgttcatg gcgtacatgg acctcacgct gctgggcgtg
    13561 acgctggtgg tgatcgtcgg tatcggagcc gtgaccgcct tgctcatgcc gcgcattcag
    13621 cgcgcgcagc tccgggcgca ggagtccgtg ggggcgatgg gcgcggccct ggaccgggtg
    13681 ctccaggcgt tccgtacggt gaaggcgagc ggcgcggagg agcgggagac ggcggcggtc
    13741 gcacgggcgg cccggcacgc gcacgaccgg ggcgtgtcgg tggcgaagtg gtcgtcggtc
    13801 tccgacgtca cgatgatgat gtcgatccag ctcgccttcc tggccgtgct gggcgtgggc
    13861 ggggcgcggg tggcctcggg ctccctggag atctcctcgc tgatcgcgtt cctgctctac
    13921 ctcttctacc tgatgggccc gatcggcggt ctggtggagg ggtggacggg gttgcagagc
    13981 ggcctcgccg ccgtacggag gatcgacgag gtggagtcgc tgccgggcga accggcggag
    14041 ggctccggcg gatcgaagga gggcggcggc ccgctgcggg tcaccttcca ggacgtcacc
    14101 ttcgggtacg gggacgagcg cgccccggcc cacaagggcg tcacgttcga ggtcccggcc
    14161 ggcgggatgg tggcgctggt gggcccgtcg ggagcaggca agtcgacggt gttcagcctg
    14221 ctggaacggt tctacgacca cgacgccggg accatcgccc tggacggccg ggacatcagg
    14281 gactggcccc tcgcggagct gcgcgcctcg ctcgcgtacg tggaacagga cgccccggtc
    14341 ctggcgggca ccctccgcga gaacctgctc ttcgcggccc ccgacgcccc cgaggagcgg
    14401 ctgacggagg cggtcacccg cacccgcctg gacgccctgg tcgcccggct gcccgagggc
    14461 ctggacacgg cggtcggcca ccggggcgtc accctctccg gcggcgaacg ccagcgcatc
    14521 gccatcgccc gcgccctgct gcgccgcccg cgcctgctgc tcctcgacga ggtgacctcg
    14581 cagctcgacg cggtcaacga acaggcactg cgcgacgtca tcctcgaact ggccgcacag
    14641 tcaacggtgt tggtcatcgc ccaccgcctc tccaccgtcc gccacgccga ccgcatcgtg
    14701 gtcctggagg acggccgcgt ccgcacggcg ggcacgcacg aggagctgat ggccggggac
    14761 gacctctacc gcgaactggc gacgacgcag ctcgcggcgg cggaggtgca ggaggcgcag
    14821 gtgcaggggg cgcggtgagg gatcaggcgg tgtagttcat cagctcctcg ctgtccaggg
    14881 tgatgtcgac ggggccgggg aggtgaacgg tgtcgccgta cgcataggtc gtctgccgcc
    14941 ggtaccggcc cttctccggc tcgctgtgca cgacaagcgt gttgtcgtcg cgatcgacga
    15001 gcagatagac ggggatcgcc gccgacgcgt atccgtcccg cttctccaca cggtcgcggc
    15061 tgtcggtgtc gtggtcgtgc gaagtgacct cgacggtcat gaggatgcgg tcgggttcgg
    15121 accactcacc gtctccggcg aagcggcctg ctcgggccag ggcgccgtcg ggccgggcgc
    15181 gaccattgcg gtacccaccg atgcgcagcc cacgctcccg gtacagccgc agctccggcc
    15241 tctgctgcat gcactgcatc gcgacccaca tgatgatctc gtcgtgatcc ccgtcgggca
    15301 cgggcttgac ctctagcttt ccgtcgatga gctcaagcgt cacggtctcc ggcgccgagc
    15361 gggcgagctg ctcgaactcc tctacggaca gctgggcccg tggtgcgtga tcggcggggc
    15421 tgggggtcat ggcgtcgcct cctcccctcc atcgtgccct gccgaatctg cggatgagcc
    15481 gcacggtcac cggtgggtcc tctcgaatcg gtaggtccgc tccgagcggg ggaccagccc
    15541 tgcccggacc gcgttcatcc gttcccgtac gccgggcggc tgcacggccc ccggcgggcc
    15601 cgccggcggc tcgggggacg gcccccggca ggcgcggcac agcccgccga gaagggcctc
    15661 cgggctgccg gggaccccgc actcgccgca ctccagggtg cgcaggacgg tccgtggccc
    15721 cggccccggc gggacgcgct cgggcggcag cttgtcggtc aggcggcgtt cgacgaggcg
    15781 ggcggcgcag tggacggggg acggcaggcc cgcggtgagg gcgtacagca gctcggcctc
    15841 ggtcgcaccc cgcgcgaacc actcgctgac ccgcggttcc agccgtacgc agtcggcgga
    15901 ggagagggag agcgtcgggg ccgtacgccc cagagcggcc agcaggatga acgcccgcga
    15961 gcgggtgggc cgttggggct ggtcgggcga ggcgtcgccc cgggtgaagg cgttccacca
    16021 ggagtcctcg cgggcggtgc gcgagaagaa cgtacgggtc acccagagca tcacgtcgtc
    16081 ggccaccacg cactcgctgc cgcgccgtaa atgcccgccg gcctggagcc ggttgagggt
    16141 ggtgctcagg gcgcactgcc cgtacgggag gtgcttggcg agcgtcttca cggagatgtc
    16201 ggagccgtcg ggcagccggt cgaggtaggc ggcgacggcg gcgtcgcggg ggaggaggtg
    16261 ggcgaagtcg cgttcggtgc gcggtcgttc gccgggggcg gagcgcttgc cgtagccggg
    16321 tttggccatg gggtgcgggg gcgcggtctc atgcgaggtg ctgtgggcag cactaaggta
    16381 gctgtcagcc atggggatcg ttgtgtccga tcttcactgg tcagacccct gttcggtgtt
    16441 acagcaccag cgggggtcgt ctttgttctg ctctcgttct ccgcacgcta aagccacgcg
    16501 acggcgcgtc gcaagtcggt cgcaaatggt caacttcggt cgcagggtgg gtgggtggga
    16561 caactgaccc ctccacccca tcactgttaa gagcgctcgg atctcgccgc ttgagctccg
    16621 ggaggggcgg ggtgggtgcg gggagggtgc ggggcgggcg tcgacgggtt cgatgggaac
    16681 gaatgtccga tttcatggag ccctctccga ccggatcggc cgtccggggg ttgtgacgac
    16741 cgtcaccggc gaactccgcg ggcgctgtag accggatgcc cggcggcagg cgtatacccg
    16801 gtgaagcgaa agatccggga gcaacttttg accgtcgagg agttcgaaga gttctacgcc
    16861 caggcggtcg cgcgtctcac aggccagctg tacgtgatga cgggggacct ccaggaggcg
    16921 caggacgtcg tgcaggaggc cttcgtcaag gcgtgggtcc ggcgcggccg gctggagcgg
    16981 gacgggcagc ccgaggcgtg gatccgtacg gtcgcgtggc gcctggcggt gagccggtgg
    17041 cgcttccggc ggcgttcggc ggacgcctgg ggccggggga gcggagcgcc ggagcacgtg
    17101 gcgccgccgg acccggacca cgtggtgctc gtcgaggcgc tccgcgggct gcccgcccag
    17161 cagcggcgga cgctgaccct gcactacctg tgcgacctca cggtcgagca gatcgccggg
    17221 gagaccggcc tttccaccag cacgatcaag acgcatctgg tccgaggccg ggcggcgctc
    17281 gcccaccggc tgcgggaccc ccgcatggag gaggctcctg atgtctgaac cacgggaccc
    17341 gtcgggcccg gaggagcccc ggggagcgca ggatccgctg aggaccctgt tccaccaggc
    17401 cggggagttc ggccagggcc gcgcggtcgc ggacccggcg gcccacatca ccgaacgcgg
    17461 catgcgcgcc cagcggcgcc ggatggcggc gctcgcggtg ggggtctgcc tggtggtcgg
    17521 cggtggcggc gcggtggcca tcggcctcca cccggtcacc ccggccccgt cccctccggc
    17581 gacgagtccg tcccccagct acccgtcccc ctcgcccccg ccgcccgccg ggactccgac
    17641 ggagccgccg acgccgagta cgacgatgcc gggggcccag gggacgggcc cctctggttc
    17701 gtacacgccc gggtcgtcgg tgccgggggg catgccgacg acggaggcgc cgacgccctc
    17761 gtcgacatcc acctccacag ccacatccac gtcggcgtcc acgtccacgt tcacggccgc
    17821 gggggcgccc ggcccgacat cgaatgagac accggcttcg acgctcgggg cgacgtcggt
    17881 gccgtgatcg tgaagggcgg cgccaggacg gcaggcggcc cgcccccgta agcactctcc
    17941 ctctccctcc cttccccctc cccgtgcccc ggcggcttca ccggacgatc cgggcggtcc
    18001 gggcggtccg ggtggtcccg cgcgcttcgg gcgcgcccga aaggttccca agtgcctata
    18061 ttgtcccctg ggttgcatcc ggccgagccc cggtcggacg caacctccgc gccccgggcg
    18121 aaccggccgg tcaagcccgt accgtacgca tccgaggcgc cccgccggga ccggtgggca
    18181 tcctggctca ccccggccga ccgcagggtg ctccagctct acctgctgac gcgcctgtcc
    18241 ctgtggacca tggcccacgg cgcccggtgg ctgtttcccc ggggctcgga tgtgcgggag
    18301 gcggcgccgc tgctcgcccc gttccagcag tgggacgcga accactacct ccacatagcg
    18361 cgcgacggct acttcccggc gggcgcgggc ccgtggctcg gcgactggga caacagggag
    18421 gcgttcttcc cgggctaccc gctcctcctg cgggcggtcc atgtcgtcgt cccggactgg
    18481 acggcggcgg gcctgctgat ctcgctggca gccggggcgg tggccgtact ggcgctctcc
    18541 cgagtagccc gctcgtacgt ccccgaggac gccgccggtc accgtacggt cctgttcttc
    18601 ctgctctccc cgtgcacggt cttcctcgcg gccggctaca cggaggccct gttcctggcc
    18661 ttcgcgctcc cggcctggct ggcggcccgg cgccaccagt gggcgtgggc ctgcgcgttg
    18721 acggctctgg cgacgacggt gcgggtcagc ggtctgttcc tggcggcggc ggtagcggtg
    18781 ctgttcgtgg tcacggcgct ccaggcgggt acggggaagg cctggcgcgg ggtggcctgg
    18841 ccggcgctcc ccgggctgcc gccactggcg tacagctggt atctgcacgc gcacacgggc
    18901 gactggatgg cctggaagca cgcgcaggag cgtggctggt accgggagtt ccacaccccg
    18961 tgggaggcgt gggcgaacac ctggaacgcg gccttcgacg gcgtgcacac gacgggctac
    19021 gcggccatgt tccaggccga gttggcggcg atgctggtgg gcgtggccct cgtcgcgctg
    19081 ctcgcacggt atggccgatg gccggaagtg gtgtacgtgg cgctgagcct gtgggcgctg
    19141 ggaacgtcgt actggtacac gtcgatcccc cgggcgacgc tgctgtggtg gccactgtgg
    19201 atcggcctgg cggcgttgag cctgcgccgc ccctggctcc gtacggcgta cctgtgcgtc
    19261 tcggccccgc tggcggccct ggtggccctg gcgttcttca cggggaggtg ggcgggatga
    19321 cccgtcgggc gcccacaggc gccggggccc cgcccgtacg ggggcaaggg aactcccacg
    19381 tactccacag gccgcgaggc atgcgctggc ggggctcagc gcatgtcgtt gacgaacgcg
    19441 gtccaggcgg gggcgccgaa gacgagggcc ggtccttccg gccgtttcga gtcgcggacg
    19501 gggatgaggc ccgggaagtc gtcggacacc tcgacgcagt tacctccgtc ggagttgctg
    19561 tacgaggact tgcgccaggt cacggaagtc aggtcggctc ggcgggaagg catgatcgga
    19621 tctccttgac caactgctgg atgaactcgg ttgtctgctc cggagagagc gccttgtctc
    19681 gctgcaaatc gtaggacagc cgcagctcct cgacgcgtcc tgagtcctca accagttcgc
    19741 cggacttgct gccctccaga taggccaccg ccttaccgtt ggccagccag aggacggtca
    19801 gcgaaccacc catcagatcg tgcagacctg ccgagaacgg cagcaactga agcgtcacgt
    19861 tcggccgctt ctgccactcc agcaaggtct ccagctgttc cagccagacg gccggctgtg
    19921 aggccgcccg gcggaacacc gcttcgtcga gcatcgcccg gaagtacgga gcgtcctccc
    19981 ccacaaggac ggactggcgg ctcatacgag cgtcgacatc ctcttgcagc tccgccgcgt
    20041 cccggggcgg cgagtgttcc agcacctccg tcgcgtacgc cctcgtctgg agcagtcccg
    20101 gaatagcgct gcatgcgtat tcgtaccgaa ccgtcgcttc ccgctccaac tgcatgaacc
    20161 gctggtaccg gctgccgaag gcgtcgtccc gggccagctc ccagagctgt tgcagctgat
    20221 cacccgtgcc gtagaccttg tcgagcgccg ccatgacctc cagcgagccc aggcgggccc
    20281 ccgtctccag cttgtgcagg tacgaccggt cgtacgtcgt ctcgtcgtgc agctcctcca
    20341 ggctcagccc ggaccggtcc cgcagccgct tcagcgtctc gtgcagcatc ttgcgggcgg
    20401 atgcctcccc gcccgaatcg gacgcgcgtt tgggcattcc aactcccctg tttcctgggg
    20461 gggatagggc tacagaaccc cccttccgag cgtagccagc aacgccgatg ctcgtgacag
    20521 aaagatccga agtccgcacg gaaggtgaga gtcatgagcg cgctgccgaa gtacgacccc
    20581 gactgccccc ggagggctga acgcatccct gatcgacccg tcatgcgcgt catcgacgta
    20641 cagagccagg agacgtactt cgacctgccg ctgatgatca ccttcgacgg aagtgcgcag
    20701 ggtccggcgt acctccgcct ggaagtggac gaggtgatcg acctcgtgga cgagctgaac
    20761 ttcgtgctgg acgtacgggt gaagggaccc gtggcgcagc cgtgcacacg gtccggcatc
    20821 tgacacgagt catgtgtggc ccgcgcgacg acgcggcgcg ggcccggaga cccacctcgg
    20881 aggacgcgac ctcatgacca tccccgtgct cgctcatcga gggcagcagg ttcacgacga
    20941 cgccgggaga agtgtgccca tgattcggcg atgccaggtc agcatcgtgc gggcgacccc
    21001 cgccggacag ccgatggtgt cggcagaccg gcgccgcccc ggacaactgc gagccatgaa
    21061 cggcgcgctc ctcacgctgt gggacctgaa gctgtgcatc gacgtggccg cgctcgtcct
    21121 cacggagctg gtgacgaacg ccttccagca cgggggcgga gaggacatca ccgtcacctg
    21181 gtgtgctgac gtcacccatc tgacgatgcg ggtggaggac agctctcccg tacgtcctgt
    21241 gctccgggcc gtggacgaga cctcggaggg cggtcgcggc ctgagcatcg tggaggcgct
    21301 ggccgacgcg tggggagtca gcgaggacgg ccatgccgtc cactgcgcgc tcgccctcgc
    21361 ggagtatgcg gcgcgggcgt gacctctgta tcgctgcggg acggtagcgg cgggagacgt
    21421 tggccgggac tcgtgcgggg cgtacggcca ttgagcgcct gcaccgagcg ccggctgccg
    21481 ccgccaggtc cggggctgtg catcctccgt cggccgtcct tcgcggtgct tcggagggtg
    21541 cgcacgccct ggggaacgag cagatggggg gcgttcgtcg gctccgccga ggtccccccc
    21601 gcacgcgcgg ggaccatacg gcgacggacc agccggcggc ggtggagaag ggaccacccc
    21661 cgcacgcgcg gggaccatgc ctggcagacc cacccgcatg atccgctcac gggaccaccc
    21721 ccgcacgcgc ggggaccatg ggccgtccga cgtacggagc gccatccgca cgggaccacc
    21781 cccgcacgcg cggggaccat ctcctcgtcc agctcgaacg cctgcggaac cggggaccac
    21841 ccccgcacgc gcggggacca tagttcctga cctgcgggtt tatgcggccg aggtgctgtt
    21901 ttccaccact tcccgggaaa cggacatatc gacctcgctt catcggcggc ccgaggcgcg
    21961 agccgagtcc ccattgttcc ggtcgtgggc acgctgtcca ccccgccgga tgagcctcgg
    22021 gccgcgcccc cgctaccgca gccgcaagct cgacgtacca ccgcccgcaa ttcagccccc
    22081 ttcaccagag ccgccccacc cccttgtcgg cggcctgcca ccccgctccc aggtgggcta
    22141 tgggtagcga gtcgacgggg tgcagccggg agtgtcggag cactgctcgt agggcgatga
    22201 gcccggggcc cgcctccggg cgtacgtgca gcccggtggc cagctcctcc gccgtgggaa
    22261 gcggtgggcg gtcgtgggac gtcagctcgt tcctggtcgc tccggtcagc catcggagaa
    22321 tgccttcgcg tacggcgtcc gcgcactccg gaccgatgtc atcggggccg agggggttga
    22381 cgaactcgaa accgaggcgg ccgtcggaca gaggctcgaa gtcgaccgtg aaccaggcga
    22441 atccacccat gcaactcggc cgggtgtagc ggacctgaac atcgcggcgg gcttcggaag
    22501 ggaatcgcat actgcaagca tccgccccga aggaactgtg ctgcagaggg acttcgctgg
    22561 tcatttcgga atcgggtccg gagccgccgc agtggccgcc ggtgagggca gtcctcctcg
    22621 ggcgaactcg gcgatcccgt acgacgactt ccgtcagcac cgcgcgtctg gcctccggga
    22681 tcaaccgtcc tgggccgtgg gagctgcgcc cacctgcttg tcggcctcgg agaacaggag
    22741 ggtggattcg gagaggatgt cgcggttgga ctcgctcttg tagacctcgt cgac
//
