LOCUS       NZ_ARPF01000016        10399 bp    DNA     linear   CON 18-FEB-2024
DEFINITION  Streptomyces sp. SolWspMP-sol2th B083DRAFT_scaffold_14.15_C, whole
            genome shotgun sequence.
ACCESSION   NZ_ARPF01000016
VERSION     NZ_ARPF01000016.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. SolWspMP-sol2th
  ORGANISM  Streptomyces sp. SolWspMP-sol2th
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 202928)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Improved High-Quality Draft of Streptomyces sp. SolWspMP-sol2th
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 202928)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name :: Streptomyces sp. SolWspMP-sol2th
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 03:17:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,475
            CDSs (total)                      :: 6,400
            Genes (coding)                    :: 6,297
            CDSs (with protein)               :: 6,297
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 31 of 103
            Pseudo Genes (incomplete)         :: 82 of 103
            Pseudo Genes (internal stop)      :: 12 of 103
            Pseudo Genes (multiple problems)  :: 20 of 103
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:24:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 124999
            Orig. end                         :: 135398
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            ARPF01000016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(425..928)
                     /locus_tag="B083_RS0126855"
     CDS             complement(425..928)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018488165.1"
                     /locus_tag="B083_RS0126855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_032756452.1"
                     /transl_table=11
                     /translation="MSRGDLAAFEELYRRTSPWLATRLRRRCADEQIVAEVMQETYLAV
                     WRAAAGFVGTATGGTAVGWLWTIAARRLVDAFRRRAHHAAPPSAAAPAPTAPAAEEEAL
                     NASVGGDIGDALSALAPELREVLRALVLDGLTVRETAVLLGLPEGTVKTRARRARIAMR
                     RALA"
     misc_feature    934..948
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.94"
                     /tool="antismash"
     misc_feature    complement(1028..1043)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.13"
                     /tool="antismash"
     gene            complement(1100..1606)
                     /locus_tag="B083_RS0126860"
     CDS             complement(1100..1606)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607784.1"
                     /locus_tag="B083_RS0126860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_018488166.1"
                     /transl_table=11
                     /translation="MNTAHAGAAEAACTDALPGAALSGPVSHAVSRVARLHRIAAGKLL
                     KGLGLYPGQELLMMHLWDAGPVRQSELIRTLDLDPSTVTKMLQRLEQTGHVRRRPDPDD
                     RRAALVEATEASCALHRAVRDAWGSLEEHTLAGLDDGERTELTRLLAKVEANLCRETEG
                     CPEGS"
     misc_feature    1831..1845
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.72"
                     /tool="antismash"
     gene            1870..2940
                     /locus_tag="B083_RS0126865"
     CDS             1870..2940
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969934.1"
                     /locus_tag="B083_RS0126865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alkene reductase"
                     /protein_id="WP_028419343.1"
                     /transl_table=11
                     /translation="MTTAFDPIDLAGTRLANRIVMAPMTRSRAGEGGTATELTVAYYTQ
                     RASAGLIVTEGIQPSAVGQGYPFTPGLHSAEQVASWRTVTDAVHAEGGRIFAQIMHAGR
                     IGHPVLLPDGLVPVAPSPVRAAGQVYTAEGPKDFVEPHELTDAEIRQTIADFAAAARNA
                     VEAGFDGVELHGANGYLIHQFLAPNTNLRTDEWGGSESARVRFAVEVVKAVAAEIGGAR
                     TALRISPSNPYNDIDEPAPDAVYTALAQEIEPLGLAYLHILEDAPIRELTLQLRKVFTG
                     SVLINVHSDGPTGPEDHTVIDDGIADLISYGVLFLANPDLPARLKAGGPFNTPDPSTFF
                     GGDAKGYTDYPALDAV"
     gene            complement(2916..3950)
                     /locus_tag="B083_RS0126870"
     CDS             complement(2916..3950)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070337.1"
                     /locus_tag="B083_RS0126870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="VOC family protein"
                     /protein_id="WP_028419344.1"
                     /transl_table=11
                     /translation="MTSHVRHITIDCADPYALGAFWSRVLGAPLAPDDFPGDPEALLET
                     PGAAVLFVRNDDPKAVKNRVHLDVQPQDRTRDEEVERLLALGATLVGDHRRPDGRGWAT
                     LTDPEGNEFCVECSAAERAALTGARLPVTADDVTTAVRLAVDALAGAPAEGWDAPAGTL
                     EWTCWETVEHLSDDLFAYAVQLGPRTPPLDGEVPYRWAPERQGGPYNAVFADRAAGPAG
                     LLATLEASGALLASMARTTPPEVRSYHGFGVSDPEGFAAMGVVETLVHTHDVAAGLGLD
                     WAPPAAVCDRVLARLFPDAPAGGDRWTVLLWATGRAGLPDHPRRSAWRWDGRPPADQTA
                     SSAG"
     gene            complement(4102..4995)
                     /gene="thpD"
                     /locus_tag="B083_RS0126875"
     CDS             complement(4102..4995)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="B083_RS0126875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_028419345.1"
                     /sec_met_domain="PhyH (E-value: 4.3e-39, bitscore: 128.5,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEVRADLYPSRGAAEMTTPRQDPVIWSAPGAPGPVAAKDLQSY
                     EHDGFLTVDQLITPDEVAVYRAELDRLVADPLIRADERSIVEKQSQNVRSVFEVHRISE
                     VFAGLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNM
                     RAVSVSIALTENFDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIRLFTGRAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENTAQEPFAAP
                     IRRPEFIGARDFTPVK"
     gene            complement(5001..5399)
                     /locus_tag="B083_RS0126880"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045195.1"
                     /locus_tag="B083_RS0126880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_018488170.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.1e-55, bitscore:
                     179.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIENTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGETHWISPGTMYLLDGHERHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEA"
     gene            complement(5489..6751)
                     /gene="ectB"
                     /locus_tag="B083_RS0126885"
     CDS             complement(5489..6751)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 2.6e-72.
                     Score: 235.6. Matches aSDomain:
                     nrpspksdomains_B083_RS0126885_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="B083_RS0126885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_028419346.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7.4e-79, bitscore:
                     258.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGKVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQELCERQDMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTLSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWADGRVEQQTKERGLQVEQALL
                     AICAEEPTAQFRGRGLVWGMEFEDPARASAVCARAFELGLLLETSGPQSEVVKLLPPLT
                     ITTEELDEGLRTLARCVRETA"
     aSDomain        complement(5663..6667)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B083_RS0126885_Aminotran_3.1"
                     /evalue="2.60E-72"
                     /label="B083_RS0126885_Aminotran_3.1"
                     /locus_tag="B083_RS0126885"
                     /protein_end="363"
                     /protein_start="28"
                     /score="235.6"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMATTAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGKVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQELCERQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGIVPDIVTLSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWADGRVEQQTKERGLQVEQALLAICAEEPTAQFRGRGLVWGMEFE"
     gene            complement(7077..7607)
                     /gene="ectA"
                     /locus_tag="B083_RS0126890"
     CDS             complement(7077..7607)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121394.1"
                     /locus_tag="B083_RS0126890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_026237552.1"
                     /transl_table=11
                     /translation="MTAAPADFARARSEFLSIDTPRVEDGAAIWRIARDSEVLDLNSSY
                     SYLLWCRDFAATSVVARGEDGEPIAFVTGYIRPDRPETLVVWQVAVDGAHRGKGLAATL
                     LDALTARVAADQGLSWVETTITPDNTASDRLFTSYAQRHDVALEREVLFDGGLFPEGTH
                     LPEVLYRIGPLTG"
     misc_feature    7618..7632
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.51"
                     /tool="antismash"
     misc_feature    complement(7632..7644)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    7719..7734
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.16"
                     /tool="antismash"
     misc_feature    7729..7743
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.76"
                     /tool="antismash"
     misc_feature    7739..7752
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.96"
                     /tool="antismash"
     misc_feature    7739..7753
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.47"
                     /tool="antismash"
     misc_feature    7773..7788
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7773..7790)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     misc_feature    7848..7864
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.46"
                     /tool="antismash"
     gene            complement(7924..9030)
                     /locus_tag="B083_RS0126895"
     CDS             complement(7924..9030)
                     /NRPS_PKS="Domain: Aminotran_5 (52-287). E-value: 2.8e-29.
                     Score: 94.1. Matches aSDomain:
                     nrpspksdomains_B083_RS0126895_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957694.1"
                     /locus_tag="B083_RS0126895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_028419347.1"
                     /sec_met_domain="Aminotran_5 (E-value: 4.4e-24, bitscore:
                     78.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLTADRFAAIERRVAALLATGQDVVITQGEALLPLE
                     GCIRSGARPGSTALNVVTGPYGQTFGNWLRDCGARVVDLTVPFHTAVTADQVAEALAGH
                     PEIDFVSLVHAEAATGNTNPVAEIGEAVRAHGALFMLDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWERFAANPAAPRRSYLSLLDWKERWIDAGRKALPHAP
                     AQLEMLGLEACLERIEEDGPDHVMARHAAAAAATRAGAAALGGGLEPYVHEARDAAPVA
                     TTLRAPAGTDAAALVARALTAEPSLPLVAGGGALSKEMIRVNHYGADATRSAVLSSLAA
                     LGAALGATGRQVDFDAARRAVSETSPDL"
     aSDomain        complement(8170..8874)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B083_RS0126895_Aminotran_5.1"
                     /evalue="2.80E-29"
                     /label="B083_RS0126895_Aminotran_5.1"
                     /locus_tag="B083_RS0126895"
                     /protein_end="287"
                     /protein_start="52"
                     /score="94.1"
                     /tool="antismash"
                     /translation="RPGSTALNVVTGPYGQTFGNWLRDCGARVVDLTVPFHTAVTADQV
                     AEALAGHPEIDFVSLVHAEAATGNTNPVAEIGEAVRAHGALFMLDAVASVGAEPLLPDA
                     WGVDLCVIGAQKAMGGPAGVSAVSVSERAWERFAANPAAPRRSYLSLLDWKERWIDAGR
                     KALPHAPAQLEMLGLEACLERIEEDGPDHVMARHAAAAAATRAGAAALGGGLEPYVHEA
                     RDAAPVATTLRAP"
     gene            9180..10292
                     /locus_tag="B083_RS0126900"
     CDS             9180..10292
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607776.1"
                     /locus_tag="B083_RS0126900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amidohydrolase family protein"
                     /protein_id="WP_063827926.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 8.8e-22, bitscore:
                     71.2, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDQVRDQERQPVLRVKGRVLVGPDEVRDELWAVGGRITYERPPG
                     AEDARTVTGWVLPGLVDAHCHVGLDAHGPVDAATAEKQALTDRGAGTLLVRDAGSPSDT
                     RWIDDREDLPKIIRAGRHIARTRRYIRNYAHEIEPGDLVAYVAREARRGDGWVKLVGDW
                     IDRSAGDLTACWPRGEVEAAIAEAHRLGARVTAHCFAEESLRDLVEAGIDCVEHATGLT
                     EETVPLFAERGVAIVPTLVNIATFPDLAAGGEAKFPRWSAHMRQLYERRYDTVRAAYDA
                     GIPVYVGTDAGGSLAHGLVADEVAELVKAGIPPLEALSATTWGARRWLGRPGLEEGAPA
                     DLVVYEEDPRADVRVLAAPRHVVLNGRVVG"
ORIGIN
        1 tccgtcaccc gcgaccagga cacggcgaca ccgagcacgg gcagcagggg cgcgatcagg
       61 acgaccgggg agaccgcccc ggcgcggtgt ccggccaggt ccatcaggag cgcgaccccc
      121 gtcaggccca ccaccgtggc cagccacggc agcgcggccg gtgtcatcca gcgcgccggc
      181 cggatccggc cgtgccgtcc cgccccggct cccggggccg ccagctcggt ggcgagcccg
      241 gtccacacgc tccgggtgag cgccgcgacg gcgggcacct gcccggccac cgcctccgac
      301 agcagcccgc ggcagtcggc gcacgcttcc agatgggatt ccaccgccca caggacgtcg
      361 gcgggcagcg ccgtgtcgcc ccgcgcgtag gcggtgatga tgtggggtga tgcgtgtcct
      421 gcggtcatgc cagcgccctc cgcatggcga tccgggcccg ccgtgcgcgg gtcttgactg
      481 tgccctcggg cagccccagc aggaccgcgg tctcgcggac cgtcagcccg tcgagcacca
      541 gggcccgcaa cacctcgcgc agttccggcg cgagcgcgct cagcgcgtca ccgatgtcac
      601 cgccgacgct cgcgttcagc gcctcctcct cggcggccgg cgcggtcggt gccggagcgg
      661 cggcggaggg cggtgcggcg tggtgggcgc ggcggcggaa ggcgtcgacc agacgccgtg
      721 ccgcgatcgt ccacagccag ccgacggccg tgccgcccgt cgcggtgccg acgaagcccg
      781 ccgccgcgcg ccagacggcg aggtaggtct cctgcatgac ctcggcgacg atctgctcgt
      841 cggcgcagcg ccggcgcagg cgggtggcga gccaggggga ggtgcgccgg tacaactcct
      901 cgaacgcggc gagatcgccc ctgctcactc tccggacgag ctgttcctcg tccagccggt
      961 cgagtgctct tctgcctgtt ctcacacctg ctagacgtgg ggatccgccc cggggttttc
     1021 ctcctcgtgt gacgtgggtc acctcggggg cgtcctggcg gtgcccggag gctttcccgc
     1081 ctccgccccc cggggagggt cagctccctt ccgggcagcc ctccgtctcc cggcagaggt
     1141 tcgcctccac cttcgccagc agccgcgtca gctccgtgcg ctcgccgtcg tccagcccgg
     1201 cgagcgtgtg ctcctccagg ctgccccacg cgtcccgtac cgcccggtgc agcgcgcagc
     1261 tcgcctccgt cgcctcgacc agcgcggccc gccggtcgtc cgggtcgggg cggcggcgca
     1321 catggccggt ctgctccagg cgctggagca tcttggtcac cgtggagggg tccaggtcca
     1381 gcgtcctgat cagctcggac tggcgtaccg gccccgcgtc ccacaggtgc atcatcagca
     1441 gctcctgccc cgggtagagc cccagcccct tcagcagctt ccccgccgcg atccggtgca
     1501 gccgggcgac gcgggagacg gcgtggctga cgggcccgct gagcgctgct ccgggcagcg
     1561 cgtcggtaca ggcggcttcc gcggcgcctg cgtgagcggt gttcatggcg gggctccttc
     1621 gatcctgctc cggcggggct tccggtggtt cctgacgggt gttcggtccc gggcgggtga
     1681 cgggtgacgg gtccggtccc ggcgggtccg ggggctgacg gcggcttccg gggtccgatg
     1741 gcgtccgagg cttgcccaca ctttaccttg gtcggccaat taatgagcta cagtgacggc
     1801 agcgccaata cttggccgac cacatacttt gtttatcggt caattaccca tctttccccg
     1861 ggagttcgca tgaccaccgc tttcgacccg atcgacctgg ccggcacccg cctggccaac
     1921 cgcatcgtga tggccccgat gacccgcagc cgggcgggcg agggcggtac ggccaccgag
     1981 ctgaccgtcg cctactacac ccagcgcgcc tccgccgggc tgatcgtcac cgagggcatc
     2041 cagccctccg cggtcggcca gggctacccc ttcacccccg ggctgcacag cgccgagcag
     2101 gtcgcctcct ggcgcacggt caccgacgcc gtgcacgccg agggcggccg gatcttcgcc
     2161 cagatcatgc acgcgggccg catcggccac ccggtcctgc tccccgacgg actcgtcccg
     2221 gtcgccccct ccccggtccg ggccgcgggc caggtctaca ccgccgaggg ccccaaggac
     2281 ttcgtcgagc cgcacgagct gaccgacgcc gagatccggc agaccatcgc cgacttcgcc
     2341 gccgccgccc gcaacgccgt cgaggccggg ttcgacggcg tggagctgca cggcgccaac
     2401 ggctacctga tccaccagtt cctcgcgccc aacaccaacc tgcgcaccga cgagtggggc
     2461 ggctcggaga gcgcccgcgt ccggttcgcg gtggaggtcg tcaaggcggt cgcggccgag
     2521 atcggcggag cccgtaccgc cctgcggatc tcgccgtcca acccgtacaa cgacatcgac
     2581 gagcccgccc cggacgccgt ctacaccgcg ctggcccagg agatcgagcc gctcggcctc
     2641 gcctatctgc acatcctgga ggacgcgccg atccgcgagc tgaccctcca gctgcgcaag
     2701 gtgttcaccg gctccgtcct catcaacgtg cactccgacg ggccgaccgg gccggaggac
     2761 cacaccgtga tcgacgacgg catcgccgac ctcatctcgt acggcgttct cttcctcgcc
     2821 aacccggacc tccccgcccg cctcaaggcc gggggcccct tcaacacccc cgacccgtcg
     2881 accttcttcg gcggcgacgc caagggctac accgactacc cggcgctcga cgccgtctga
     2941 tccgccgggg gccgcccgtc ccaccgccac gcggagcggc gcgggtggtc cggcagtccg
     3001 gcgcggccgg tggcccagag cagcacggtc caccggtcgc cgcccgccgg ggcgtcgggg
     3061 aagagccggg ccagcacccg gtcgcagacc gcggccggcg gcgcccagtc cagccccagc
     3121 cccgccgcga cgtcatgggt gtgcaccagg gtctccacca cccccatcgc ggcgaacccc
     3181 tccgggtcgg agacgccgaa cccgtggtac gagcgcacct ccggcggcgt cgtccgcgcc
     3241 atcgaggcca gcagcgcacc gctcgcctcc agagtggcca gcagcccggc gggcccggcc
     3301 gcccggtccg cgaagaccgc gttgtacggc ccgccctgcc gctcgggcgc ccaccggtag
     3361 ggcacctcac cgtcgagcgg gggcgtacgc gggcccaact ggacggcgta cgcgaagaga
     3421 tcgtcgctca ggtgctccac cgtctcccag caggtccact ccagcgtccc cgcgggcgcg
     3481 tcccacccct ccgccggggc cccggccagc gcgtccaccg cgagccgcac ggcggtggtc
     3541 acgtcgtcgg cggtcaccgg cagccgggcc ccggtcagcg cggcccgctc ggcggcggag
     3601 cactccacac agaactcgtt gccctccgga tccgtgaggg tcgcccaccc ccgcccgtcc
     3661 ggtctgcggt ggtcgcccac gagcgtggcg cccagcgcga gcagccgctc cacctcctcg
     3721 tcccgggtgc ggtcctgcgg ctggacgtcc aggtggaccc ggttcttgac ggccttcggg
     3781 tcgtcgttcc ggacgaagag gaccgccgcg ccaggggtct ccagcagcgc ttccgggtcg
     3841 ccggggaagt cgtccggggc cagcggcgcg ccgagcaccc gggaccagaa ggcgccgagc
     3901 gcgtaggggt cggcgcagtc gatcgtgatg tgccgtacgt gagaagtcat ggccgccacc
     3961 ctctcctctg ccccggggcc cggtccaccg ggttatgcgc gagggccggg aacgggtcac
     4021 gcgcgaggag ccggcccggg gtacacggcg ggggcccggg acggtgtgct gtcccgggcc
     4081 cctcgcgcgt ggcacgtccc gctacttcac cggggtgaag tcccgcgccc cgatgaactc
     4141 cgggcggcgg atcggtgccg cgaacggctc ctgcgccgtg ttctccacgc tgttgaagac
     4201 gatgaagacg ttggagcggg cgtacggggt gatgttgtcg cccgagccgt gcatcgcgtt
     4261 gcagtcgaac caggtcgccg aaccggcccg gcccgtgaag agcctgatgc cgtggcggtc
     4321 ggccatcttc gtcagcgcct cgtcggacgg ggtgcccgcg tcctgcatct ggagcgactt
     4381 cttgtagttg tccttcggcg tctcgcccgc acagcccagg aaggtcttgt gcgaaccggg
     4441 catgatcatc agcccgccgt tggtgtcgaa gttctccgtc agcgcgatcg acaccgacac
     4501 ggcccgcatg ttcggcagcc cgtcctcggc gtgccaggtc tcgaagtccg agtgccagta
     4561 gaagcccgag gcgccgaacc ccggcttcac gttgatccgg gactggtgca cgtacacgtc
     4621 cgagccgagg atctggcggg cccggcccac cacccgctcg tcgcggacca gcccggcgaa
     4681 gacctcgctg atcctgtgca cctcgaagac ggaccggacg ttctgcgact gcttctcgac
     4741 gatggagcgc tcgtcggcac ggatcagcgg gtcggcgacc agccggtcca gctccgcgcg
     4801 gtagacggcg acctcgtccg gggtgatgag ctggtcgacg gtgaggaagc cgtcgtgctc
     4861 gtagctctgg agatccttgg cggcgaccgg gcccggcgcg cccggcgccg accagatgac
     4921 cgggtcctgg cggggagtgg tcatctcggc ggcgccgcgc gaggggtaca gatcggcgcg
     4981 tacttcggtg gtcatacggt tcaggcctcc tcggtcagca gtgggtagac accgttctcg
     5041 tcgtggtcct cccgtccggt gacgggaggg ttgaagacgc acacgcagcg gaagtcggtc
     5101 ttgggccgca gggtgtgccg ctcatggccg tccagcaggt acatcgtgcc gggggagatc
     5161 cagtgggtct cgccggtctc gtcgttggtc agctcggcct cgccctcggt gcacaggacg
     5221 gcctcgatgt ggttcgcgta ccacatcgag gtctccgtgc ccgcgtagag cacggtctcg
     5281 tggagggaga agccgacctt ctccttggcg agcacgatgc gcttgctctc ccaggtgccg
     5341 gacgcggcct tcacatgccg gtcggtgttc tcgatgtcct tgaacgatcg gacgatcacg
     5401 gtgagcggtg cctttctctc gtgctccgcc ccgggccgtg aggcccgggg cgcctcttct
     5461 tcctgccgcc tgccccggcc gcgccgggtc aggccgtctc gcggacgcag cgggccagcg
     5521 tgcgcaggcc ctcgtccagt tcctcggtgg tgatggtcag cggcggcagc agcttgacga
     5581 cctcgctctg cgggcccgag gtctccagca ggagccccag ctcgaaggcg cgggcgcaga
     5641 ccgccgaggc gcgcgccggg tcctcgaact ccatgcccca gaccaggccc cggccgcgga
     5701 actgcgcggt cggctcctcg gcgcagatcg ccagcagcgc ctgctcgacc tggaggccgc
     5761 gctccttggt ctgctgctcc acccggccgt cggcccagta ggcgtccagc gcggcggcgg
     5821 ccgtgacgaa ggccgggttg ttgccgcgga aggtgccgtt gtgctcgccc ggctcccaga
     5881 tgtccagctc cggcttgaac aggcagagcg acatcggcag gccgtagccg ctgatggact
     5941 tcgacagcgt gacgatgtcc gggacgatcc cggcctcctc gaaggagaag aagccgcccg
     6001 tccggccgca gcccatctgg atgtcgtcga cgatcagcag catgtcctgg cgctcgcaca
     6061 gctcctggag ggcgcgcagc cactcggcgc gggccacgtt gatgccgccc tcgccctgta
     6121 cggtctccac gatcacggcg gcgggcttgt tgagcccgga gccctggtcc tccagcagcc
     6181 gctcgaacca gaggaagtcg gggaccttgc cgtcgaagta gttgtcgaac ggcatcggcg
     6241 tgccgtgcac cagcgggatg ccggcgcccg cccgcttgaa cgcgttgccg gtgacggcga
     6301 gcgagcccag cgacatgccg tggaaggcgt tggtgaacga caccacggac tcgcggccct
     6361 tgaccttgcg ggccagcttc agcgccgact cgacggcgtt ggtgcccgtc gggccgggga
     6421 acatcacctt gtacggcagg tcgcgcgggc gcaggatcac gttctggaac gtctccagga
     6481 aggcgcgctt ggccgtggtc gccatgtcca ggccgtgggt gatgccgtcg cgctcgatgt
     6541 agtcgatcag cgctcgtttc agcaccgggt tgttgtggcc gtagttgagc gagccggccc
     6601 cggcgaagaa gtccaggtag gagtggccgt cctcgtcggt cagccgggcg ccctgcgcgc
     6661 ggtcgaacac ggcgggccag ccgcggcagt agctacggac ctccgactcc agggtctcga
     6721 agacgctcag tgcgggcggg gtgatggtca cagcggatct cctgcgtgag ggggtgtgac
     6781 gggcggtgtg gtcggtgctc ccggccccgc gggggcggcc gggaccgcgc cgggtggtcg
     6841 gccggcgcga aaagtcgtgg gggaccgggc gtacgggtgc ggtacgccgg tcgtgcgggg
     6901 gtgtacgggc cgtgcggtcg tacgggaccg tccggacccg ggtattccgg gcgggccggg
     6961 cgggtcgtct ccggcggccg ggcggtggcc cggccggtgg ggccgggcgc ctcggacgcc
     7021 cggggccgcc gcggcggcgc cccgttgccc cggggctcgg tggcgccccg ggcacctcag
     7081 ccggtgagcg gtcctatccg gtagaggacc tcgggcaggt gcgtcccctc ggggaacagc
     7141 ccgccgtcga agagcacctc ccgctccagc gccacgtcgt gccgctgggc gtaggacgtg
     7201 aacagccggt cggacgcggt gttgtcgggg gtgatcgtcg tctcgaccca ggagagcccc
     7261 tggtcggcgg cgacccgggc ggtcagcgcg tccaacagtg tggcggccag ccccttgccg
     7321 cggtgcgccc cgtcgacggc cacctgccag accaccaggg tctcggggcg gtcgggccgg
     7381 atgtagccgg tgacgaaggc gatcggctcg ccgtcctcgc cacgggcgac gacggacgtc
     7441 gcggcgaagt cacggcacca cagcaggtag ctgtacgagg agttcaggtc caggacctcg
     7501 gagtcgcggg cgatgcgcca gatcgcggct ccgtcctcca ctcgtggcgt gtcgatactc
     7561 aggaattcgc ttcgggcacg ggcaaaatct gctggtgcgg cggtcatgtg aattgaattt
     7621 acccagcaaa tttcgaaatt gcatcgaggc aaggggttgg gtggagcgct cgtctgtgtt
     7681 atcaggcgtg cgtgagaccc ggggtgatcg aggggcgatt cagtgctttt tgtcctgaat
     7741 ttttcaggca taacgccgag ctagtggagt cggtcacaag gtggtaactc cttcggaacg
     7801 tgtccgaatc atgaagttcg ggccccggaa aaacttctgc gtttagggga cggaaaagcg
     7861 ggcagaagaa tgcggggagc tgatttcgat aaagcagctc cccgcatttt gaatatcaga
     7921 ggctcagagg tccggagagg tttccgagac cgcgcggcgg gcagcatcga agtcgacctg
     7981 ccggcccgtg gcgccgagcg ccgcacccag ggccgcgagc gaggacagca ccgcgctccg
     8041 cgtcgcgtcc gccccgtagt gattgacccg gatcatctcc ttggacagcg ccccgccgcc
     8101 cgcgacgagc ggcagcgagg gttccgccgt gagcgcccgc gcgaccagcg cggcggcgtc
     8161 ggtccccgcc ggggcgcgca gcgtggtcgc caccggagcg gcgtcccgcg cctcgtggac
     8221 gtacggctcc agcccgccgc cgagggccgc cgcgcccgcc cgggtcgccg ccgcggccgc
     8281 ggcgtgacgg gccatcacgt ggtccgggcc gtcctcctcg atccgctcca ggcacgcctc
     8341 caggcccagc atctccaact gcgccggggc gtgcgggagc gccttgcggc ctgcgtcgat
     8401 ccagcgctcc ttccagtcca ggagcgagag gtaggagcgg cggggggcgg ccgggttggc
     8461 ggcgaaccgc tcccaggccc gctcgctcac cgacaccgcc gagaccccgg ccggaccgcc
     8521 catcgccttc tgcgcgccga tcacgcacag gtccacgccc caggcgtccg gcagcagcgg
     8581 ctcggcgccc accgaggcga ccgcgtccag catgaacagc gccccgtgcg ccctgaccgc
     8641 ctcaccgatc tccgcgaccg ggttggtgtt gccggtggcc gcctccgcgt gcaccagcga
     8701 gacgaagtcg atctccgggt gcccggcgag cgcctcggcg acctggtcgg cggtgaccgc
     8761 cgtgtggaag ggcacggtga ggtcgacgac ccgggcgccg cagtcccgca gccagttgcc
     8821 gaaggtctgc ccgtacggcc ccgtgaccac gttcagcgcg gtcgagccgg gccgggcgcc
     8881 gctgcggatg cagccctcca gcggcagcag cgcctcgccc tgtgtgatga cgacgtcctg
     8941 cccggtggcg aggagcgcgg ccacccgccg ctcgatggcc gcgaagcggt cggcggtcag
     9001 cggcgggagg tcgaggaacg ggtgcgtcac ggtggggctc tcttcggatc ggggccgtgg
     9061 tcgggcggcg gtgctgcgga gcggccgtcg ccccctcggg ggagtgccgc gcccaggggt
     9121 gtccggctcg gtcccgacgc ccccctcgca gcctaccgat ggcctcgtac agtgccgcta
     9181 tgagtgatca ggtgcgcgat caggagcggc agccggtgct gcgcgtgaag gggcgggtcc
     9241 tcgtcggtcc ggacgaggtg cgcgacgaac tgtgggccgt cggcgggcgg atcacctacg
     9301 agcggccgcc gggcgccgag gacgcgcgga ccgtgacggg atgggtgctg cccggtctcg
     9361 tcgacgcgca ctgccatgtg gggctggacg cgcacggccc ggtcgacgcg gccacggcgg
     9421 agaagcaggc gctcaccgac cggggcgccg ggaccctgct cgtccgggac gccggatcgc
     9481 cctccgacac ccgctggatc gacgaccgcg aggacctgcc gaagatcatc cgggcgggcc
     9541 gccacatcgc caggacccgc cgctacatcc gtaactacgc ccacgagatc gagcccggcg
     9601 acctcgtcgc gtacgtggcg cgggaggccc ggcgcgggga cggctgggtc aagctggtgg
     9661 gggactggat cgaccggtcc gccggggacc tcaccgcctg ctggccgcgc ggcgaggtgg
     9721 aggcggccat cgcggaggcg caccggctgg gcgcccgcgt caccgcgcac tgcttcgcgg
     9781 aggagtcgct gcgcgatctg gtcgaggcgg gcatcgactg cgtcgagcac gccaccggcc
     9841 tcaccgagga gaccgtcccg ctcttcgccg aacgcggggt cgccatcgtc ccgaccctgg
     9901 tcaacatcgc cacgttcccc gacctcgcgg cgggcggcga ggccaagttc ccccgctggt
     9961 cggcccatat gcggcagctg tacgagcgcc ggtacgacac cgtgcgcgcc gcgtacgacg
    10021 ccggaatccc ggtctacgtc ggcaccgacg cgggcggctc gctggcgcac ggcctggtcg
    10081 cggacgaggt ggccgagctg gtgaaggcgg gcatcccgcc cctggaggcc ctctccgcca
    10141 ccacgtgggg ggcgaggcgg tggctggggc ggccggggct ggaggagggc gctccggcgg
    10201 acctggtcgt gtacgaggag gacccgcgcg ccgacgtccg ggtgctggcg gccccgcgcc
    10261 atgtggtgct caacgggcgg gtggtggggt gagccggggc cggggcgccc gggggtgacg
    10321 ggttgaccgg gggtgacgta acccgccccc ggctcgttga acgtctttac gcggcccccg
    10381 gtacggtcgc acatcgttc
//
