LOCUS       NZ_ARPF01000008        11440 bp    DNA     linear   CON 18-FEB-2024
DEFINITION  Streptomyces sp. SolWspMP-sol2th B083DRAFT_scaffold_7.8_C, whole
            genome shotgun sequence.
ACCESSION   NZ_ARPF01000008
VERSION     NZ_ARPF01000008.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. SolWspMP-sol2th
  ORGANISM  Streptomyces sp. SolWspMP-sol2th
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 437274)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Improved High-Quality Draft of Streptomyces sp. SolWspMP-sol2th
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 437274)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name :: Streptomyces sp. SolWspMP-sol2th
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 03:17:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,475
            CDSs (total)                      :: 6,400
            Genes (coding)                    :: 6,297
            CDSs (with protein)               :: 6,297
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 31 of 103
            Pseudo Genes (incomplete)         :: 82 of 103
            Pseudo Genes (internal stop)      :: 12 of 103
            Pseudo Genes (multiple problems)  :: 20 of 103
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:24:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 138444
            Orig. end                         :: 149884
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            ARPF01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
FEATURES             Location/Qualifiers
     region          1..11440
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11440
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11440
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6440](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6440)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1277..2005
                     /gene="hemQ"
                     /locus_tag="B083_RS0119005"
     CDS             1277..2005
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="B083_RS0119005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_028418094.1"
                     /transl_table=11
                     /translation="MSAPETVTPSKSPNAGKKAKDLNEVIRYTLWSVFKLRDVLPLDRT
                     GYADEVQELFDQLAAKDVTVRGTYDLSGLRADADLMIWWHAETSDQLQDAYNLFRRTRL
                     GRALEPVWSNMALHRPAEFNKSHIPAFLADETPRDYVSVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARLHVRE
                     EIPFYTGRRKDIADLVAGLA"
     gene            complement(2167..3735)
                     /locus_tag="B083_RS0119010"
     CDS             complement(2167..3735)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (105-466). E-value:
                     9.9e-17. Score: 53.3. Matches aSDomain:
                     nrpspksdomains_B083_RS0119010_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956907.1"
                     /locus_tag="B083_RS0119010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_028418095.1"
                     /sec_met_domain="PF00561 (E-value: 9.7e-22, bitscore: 72.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAVPLYATIGSLVLSALAGTAPAAASERNGGGSAEERGTAVAAA
                     RAAAAGISFGACPEEEMFPDSLECGTVRVPLDYARPDGPAVPLTVSRTRATGEPAQRQG
                     SFVYNPGGPGASGITFPLLGEMSEWKEVAEAYDLVGYAPRGVGTSAPLSCQDPAVFAQS
                     PTDSPAHPTTAYKEKRIAQARAYARGCEESAGEALRHYTSLNNARDLEVLRAALGDERL
                     TFMGASYGTYIGSLYATLFPARVRRMVFDSAVDPDPEKIWYRSNLDQSLAFESRWEDFR
                     RWVARHDDVYGLGASPEAVQRHYEDTRTALAAEPAGGTVGPGQLHAGFLQAAYYDDYWA
                     MRATALSEYVRGNPEPLVTQLSPRAVTAKDDENERAVYTAVECNDAPWPEEWEVWDRDH
                     TDLARIAPFQTWHNAFTNLPCAFWPAPRQRPLDVSTGWGELPPVLILAAERDAAAPYQG
                     ALELQRRLRGAVLVTERGAGTHGVGGFGNACVDDHMRRYLLTGGTPAGDAECAPRAEPN
                     PVSLD"
     aSDomain        complement(2338..3420)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B083_RS0119010_Abhydrolase_1.1"
                     /evalue="9.90E-17"
                     /label="B083_RS0119010_Abhydrolase_1.1"
                     /locus_tag="B083_RS0119010"
                     /protein_end="466"
                     /protein_start="105"
                     /score="53.3"
                     /tool="antismash"
                     /translation="FVYNPGGPGASGITFPLLGEMSEWKEVAEAYDLVGYAPRGVGTSA
                     PLSCQDPAVFAQSPTDSPAHPTTAYKEKRIAQARAYARGCEESAGEALRHYTSLNNARD
                     LEVLRAALGDERLTFMGASYGTYIGSLYATLFPARVRRMVFDSAVDPDPEKIWYRSNLD
                     QSLAFESRWEDFRRWVARHDDVYGLGASPEAVQRHYEDTRTALAAEPAGGTVGPGQLHA
                     GFLQAAYYDDYWAMRATALSEYVRGNPEPLVTQLSPRAVTAKDDENERAVYTAVECNDA
                     PWPEEWEVWDRDHTDLARIAPFQTWHNAFTNLPCAFWPAPRQRPLDVSTGWGELPPVLI
                     LAAERDAAAPYQGALELQRRL"
     gene            complement(3961..4992)
                     /locus_tag="B083_RS0119015"
     CDS             complement(3961..4992)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514514.1"
                     /locus_tag="B083_RS0119015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_028418096.1"
                     /transl_table=11
                     /translation="MNTFALLLTLAVVLSTALLIAKAVASRSGRRTPVGSPYPVRGLYE
                     AAFLNGGPARVVDTALTTLYGDGRLLIGGPGIVSVRREQAHDPVEHAVFQVLATAPSGA
                     LHAVREAVMRHPAVQGIGDGLAERGLLVPPAASRPVRRWGLIQGVSCIVGFPLAIAATV
                     IQYVNHDSPFDMPFPFVLKVLPAILLGGISGLVVAAAAAGRLTRAGRRAAHEFRTAHAY
                     QVTPPYLVATQGLRALPDPVLQAQLIAAAGANGPSGRSRHRYRTAGVVGGTAAAGGFAA
                     TTVWCADAGPGHTSCGGSSGDGSGSGCGGGSGCGGSGSSCGGGSSCGGGSSCGGGGGCG
                     GGS"
     gene            complement(5001..6440)
                     /locus_tag="B083_RS0119020"
     CDS             complement(5001..6440)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491673.1"
                     /locus_tag="B083_RS0119020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_028418097.1"
                     /sec_met_domain="DUF692 (E-value: 3.6e-93, bitscore: 306.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLGIGIGWRPEIAAEVEALTGIDWVEAVAENLCADHLPDSLVRL
                     RERGVTVVPHGVSLGLGGADRPDPGRLAGLAARAELLGTPLVTEHIAFVRAGGPLTASP
                     RLEAGHLLPVPRTWDALDVLCENVRIAQDALPVPLALENIAALVSWPGEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNRGEDPAKALDELPVEAIAYVHVAGGVEKDGVWHDTHA
                     HPVTAPVLDVLAELRSRVDPPGVLLERDDAFPPGAELAAELATIRGTLRAAAAVAPAPR
                     TGAAVAAAAVDPPASTSTAPPASTAPPALTAPPASAAPSADARAGAPGGALARTALPPG
                     IPRPGARDRTAVAQTALLSALVAGTPAPEGFDHRRLRVQSRALAAKRADVVAKVAPELP
                     EILGDGYRAAFLPYAMSRPMNGGYRRDAMEFAERILVSGGLPDPVAQRRLTHWWEDRSG
                     PRPPGRATRLVRAARAALAGK"
     gene            complement(6566..7312)
                     /locus_tag="B083_RS0119025"
     CDS             complement(6566..7312)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072530.1"
                     /locus_tag="B083_RS0119025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_028418098.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVATVGALAFPVWSYADRSGTGEANLNASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPSEATKAAGDHLVVGHTDLDRRAR
                     DVAAKLGVELPNQPTEQQQAWLRELTAASGDEYERKFANILRAAHGKVFGLIGQVRHTT
                     RNTLIRQLASDANQTVLDHITMLEATGFVDFDALAREAAGGSTASPSGPPAPREGQVPL
                     APVPVTPSGDQSFTSRPVPPTMPPMP"
     misc_feature    complement(7326..7341)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     misc_feature    complement(7337..7352)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.94"
                     /tool="antismash"
     gene            complement(7655..8491)
                     /locus_tag="B083_RS0119030"
     CDS             complement(7655..8491)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491671.1"
                     /locus_tag="B083_RS0119030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_032755858.1"
                     /transl_table=11
                     /translation="MSSDDTSPSHSGDISPGPLAEGPAGSAGPTGSAGPTASAGPTASA
                     RPADRDAAPQYVLPLVLHLEKTDPPARTDALRTAARAVLTILADERSTGEGEWARAMRD
                     WEDARIRKVVRRARGAEWRKAAALPGITVTGGSAEVRVYPPIPLDGWPKELAKLQVSGT
                     DLDDPEPPAAPGPEGPVLWLNPELEMSAGKTMAQVGHGAQLAWWELSETDRKAWREAGF
                     PLSVATAAPDRWRELVTSGLPVVRDAGFTEIAPGPTVVAEGGERFCPLPGGRPGRR"
     gene            8563..9237
                     /locus_tag="B083_RS0119035"
     CDS             8563..9237
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378552.1"
                     /locus_tag="B083_RS0119035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AIM24 family protein"
                     /protein_id="WP_028418100.1"
                     /transl_table=11
                     /translation="MKSDLFSSEHLAQPATAPGMTLQNSKSIKYAVNGEMHARQGSMIA
                     FRGNLQFERKGQGIGGLLKRAVTGEGLPLMAVRGQGEAWFAHEAANCFIVEMDQGDQLT
                     INGRNVLCFDATLSYEIRTVKGAGMVGGGLFNSVFSGYGKIGLMCEGTPIVIPVTGAQP
                     VYVDTDAVVGWSQHLTTTLHRSQSVGSMVRGGSGEAVQLMLQGEGFVIVRPSEARPDRT
                     AN"
     gene            9280..9477
                     /locus_tag="B083_RS0119040"
     CDS             9280..9477
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049036.1"
                     /locus_tag="B083_RS0119040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_028418101.1"
                     /transl_table=11
                     /translation="MDDNASYCETPASVPASAAPDGDAGPPFAECVLCREPTEYPESAK
                     GATLCPVCAWQEAGRTACSG"
     gene            9769..10761
                     /locus_tag="B083_RS0119045"
     CDS             9769..10761
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947698.1"
                     /locus_tag="B083_RS0119045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="WP_028418102.1"
                     /transl_table=11
                     /translation="MSAYFETATGDLASEALHSFADVHADLVSADSRFGFLTARHPAAT
                     RTVGLLSGGGSGHEPLHGGYVGRGMLDAAVPGRVFASPHNRQVYEASLAVARPGGVLHL
                     VKNYTGDRINFGIAAERLAHKGIPCARVLIDDDLASDAEGIAAGRRGTGGTVLVEKILG
                     GAADRGMGLAELQDLGERLVAGCRSLAVATAAHTSPATGGPAFTLDEGLLEYGVGIHGE
                     RAGSTVSRRPLAELVERMTTELLDALPADVPVISLVNGLGATTLLELYAAHGQLARVLR
                     GAGVPLARSLVGNYITSLDMHGLSITLMAADPTALGLYDAPVHTPALRW"
ORIGIN
        1 gccgtcggcc tctccggcag cctccagccg cccgccaccg ccaccgcctc cctctggacc
       61 cgcgacgcgc tgcgcccgat gcccaagggc catgtcatgg gcgtccccgg cgacccggcc
      121 gccctggagg gtgtgctctc ccccgagggg ctggcccgga tcgcccagga gcgcgacctg
      181 accccgacgc ccgtcgggga cgacgtggcc gtcggcgcgt acgtggcgga ccggctcggc
      241 cgcgaggtcg tcgaccggct ggtggaaccc ctcctcggcg gggtctacgc gggcgacgcc
      301 taccggatct cgctgcgcgc cgccgtaccg cagctcttcg aggccgtgca ggagggcggc
      361 ccgctgctgg acgccgtccg ccgcatccag gagcgcgccg cggcccggca gcagaccggc
      421 cccgtcttcc agggcctccg cggcggcatc ggcaccctgc ccggggccgt gggcgatgcc
      481 gtacgggccg ggggcggcga gatcctgacc gagaccccgg tcctcggcct gacccgtacg
      541 gccacggggt ggacggtccg caccgacacc ggcacgatca ccgccgacgg tgtcgtcctc
      601 gccacccccg cctggtccgc ctccgccctg ctggccgccg agtccccggc cgccgccacc
      661 gagctgaccg gtgtcgagta cgcctcgatg gccctggtca ccatggcctt ccggcgcgcc
      721 gacatcgagg ggttggaggc cctgcgcggc cgctccggct tcctcgtacc accggtcgac
      781 ggccgcacca tcaaggcctc caccttctcc agcaacaagt ggcagtgggt cgcggacgcc
      841 gcccccggcc tcttcgtgct ccgcacctcc gtcggccgct acggcgagga ggagcacctg
      901 caccgcgagg acggcgaact cgtcgccgtc tccctgcggg acctgcgcgg ggccaccggg
      961 ctcgccgccc gccccgtcga caccgaggtc acccgctgga tcgggggcct cccgcagtat
     1021 ccggtcggcc accacacccg cgtcgcccgc atccgcgacg aggtcgcgaa actgcccgcg
     1081 ctccgcgtct gcggcgcggc ctacgacggg gtcggcatcc ccgcctgcgt cgcgagcgcg
     1141 cggcgggcgg ccgacgagat cgcccgggac ctgacgggga ggaccacgag cgatccggcg
     1201 ggggagatca cgagcgagat catcgccacg ccgaccctgg ttcggggcac cgggagcgag
     1261 gcgggacaat agccgtatga gtgctcctga gactgtgaca cccagcaaga gtccgaacgc
     1321 cggaaagaag gccaaggacc tcaacgaggt catccgctac accctctggt cggtcttcaa
     1381 actccgcgac gtgctgccgc tggaccggac gggatacgcg gacgaggtcc aggagctgtt
     1441 cgaccagctc gcggccaagg acgtcaccgt gcgcggcacc tacgacctct ccggtctccg
     1501 cgccgacgcc gacctgatga tctggtggca cgccgagacc tcggaccagc tccaggacgc
     1561 ctacaacctc ttccgccgca ccaggctcgg gcgcgctctc gaaccggtct ggtcgaacat
     1621 ggccctgcac cggcccgccg agttcaacaa gtcgcacatc ccggcgttcc tcgccgacga
     1681 gaccccgcgc gactacgtca gcgtctaccc cttcgtacgg tcctacgact ggtacctgct
     1741 gccggacgag gaccgccgcc gcatgctcgc ggaccacgga aagatggccc ggggctaccc
     1801 ggacgtccgc gccaacaccg tcgcctcctt ctccctgggc gactacgagt ggatcctggc
     1861 cttcgaggcg gacgagctgt accgcatcgt cgacctgatg cgccacctgc gcgcctccga
     1921 ggcacggctc cacgtgcgcg aggagatccc gttctacacg ggccgccgga aggacatcgc
     1981 agacctggtg gccgggctcg catagccagc gagttcccgc accaccccaa cccggaagat
     2041 cagaagacgg gcatcgggca ccgaccgtgc cgcccgccgt tccagcgaca ccgggttcgg
     2101 cttcgggccc tgtgggcgca ccgagagtgc gcccacaggg cccgtttcca cacgtcggac
     2161 aggcccctag tccagcgata cggggttcgg ctccgcgcgc ggggcgcact ccgcgtcccc
     2221 ggcgggcgtc ccgcccgtca gcagataccg ccgcatgtgg tcgtccacgc aggcgttccc
     2281 gaacccgccg accccgtgcg tccccgctcc ccgctccgtc acgagcaccg cgccgcgcag
     2341 ccgccgctga agttccaggg cgccctggta cggggccgcc gcgtcgcgct ccgccgccag
     2401 gatcagcacc ggcggcagct cgccccaccc cgtggagacg tccaggggcc gctgccgggg
     2461 cgcgggccag aaggcgcacg gcaggttggt gaaggcgttg tgccaggtct ggaagggcgc
     2521 gatgcgggcg aggtccgtgt ggtcgcggtc ccagacctcc cactcctcgg gccacggcgc
     2581 gtcgttgcac tcgacggcgg tgtagacggc ccgctcgttc tcgtcgtcct tcgccgtgac
     2641 cgcccggggc gacagctgtg tgaccagcgg ctccgggttc ccccggacgt actcggagag
     2701 ggccgtcgcc cgcatcgccc agtagtcgtc gtagtacgcg gcctggagga accccgcgtg
     2761 gagctgcccc ggccccaccg tcccgcccgc cggttcggcc gcgagcgcgg tgcgcgtgtc
     2821 ctcgtagtga cgctgtacgg cctcggggga ggcgccgagc ccgtagacgt cgtcgtgccg
     2881 ggcgacccag cgccggaagt cctcccaccg ggactcgaag gcgagcgact ggtcgaggtt
     2941 ggagcggtac cagatcttct ccgggtccgg gtcgaccgcg gagtcgaaga ccatccggcg
     3001 gacgcgcgcg gggaagaggg tcgcgtacag ggagccgatg tacgtgccgt acgaggcgcc
     3061 catgaaggtc agccgctcgt ccccgagggc ggcccgcagc acctccaggt cacgggcgtt
     3121 gttgagcgag gtgtagtggc gcagtgcctc gcccgcgctc tcctcgcacc cccgcgcgta
     3181 cgcccgcgcc tgggcgatcc gcttctcctt gtacgcggtg gtggggtggg cgggggagtc
     3241 ggtggggctc tgcgcgaaga cggcggggtc ctggcaggag agcggggcgg aggtgccgac
     3301 gccgcgcggg gcgtagccga ccaggtcgta ggcctcggcc acctccttcc actcggacat
     3361 ctcgcccagc agcgggaagg tgatgccgga ggcgccgggc ccgcccgggt tgtagacgaa
     3421 cgagccctgc cgctgggcgg gttcaccggt ggcgcgggta cggctcacgg tgagcgggac
     3481 ggccgggccg tccgggcggg cgtagtccag ggggacccgt acggtgccgc actccagcga
     3541 gtccgggaac atctcctcct ccgggcacgc cccgaaggag atccccgccg ccgcggcgcg
     3601 ggccgccgcc accgccgtac cgcgctcctc ggcggagccg ccgccgttcc tctccgaagc
     3661 ggcggccggt gcggtgccgg ccagcgccga caggaccagc gagccgatcg tcgcgtacag
     3721 ggggactgct ctcacggcgg ggcctctctg cggatgcgga gagggatggt gggggtgggg
     3781 gcgggtgaag tccactaccg ggcgggcgtg ttgcgcggat caacccgttc gcgccacctg
     3841 aagggtgagg accccgaacc gctcgcgggc tgacggctcg gtaccggcct cccggggtga
     3901 cggctcagga tccgcctccg agcactgacg gctcaggatc cgcccccgag cgccgacggc
     3961 tcaggacccg cccccgcagc cgcccccgcc tccgcagctg gagcccccgc cgcagctgga
     4021 cccgccgcca cagctcgacc cggagccgcc gcacccggac cctccgccgc acccggaacc
     4081 cgacccgtcg ccgctggacc cgccgcaact cgtgtgcccg gggcccgcgt cggcgcacca
     4141 caccgtggtg gccgcgaagc ccccggccgc tgccgtaccg ccgacgaccc ccgccgtccg
     4201 gtaccggtga cgcgaccgcc cggacggtcc gttcgccccg gccgccgcga tcagctgggc
     4261 ctggagcacc gggtcgggga gggcgcgcag cccctgggtg gcgacgaggt acggcggggt
     4321 gacctggtag gcgtgggcgg tccggaactc gtgggcggcg cggcgccccg cccgggtgag
     4381 ccggcccgcc gcggccgcgg ccacgacgag cccgctgatc ccgccgagca ggatcgcggg
     4441 cagcaccttc aggacgaacg ggaacggcat gtcgaacggc gagtcgtggt tcacgtactg
     4501 gatgaccgtg gcggcgatgg cgagcgggaa gccgacgatg cagctcaccc cctggatcag
     4561 tccccagcgc cggacgggac ggctcgcggc gggcggcacc agcagcccgc gctccgccag
     4621 accgtccccg atcccctgca ccgccggatg gcgcatgacc gcttcccgta ccgcgtgcag
     4681 ggcgccgctg ggcgcggtcg ccagcacctg gaacacggcg tgctccaccg ggtcgtgcgc
     4741 ctgttcacgc cgtacggaga cgatcccggg gccgccgatg agcaggcgcc cgtccccgta
     4801 cagggtggtg agcgcggtgt ccacgaccct ggccgggccg ccgttgagga acgccgcctc
     4861 gtagaggccg cggacggggt agggggagcc gacgggggtg cggcggcccg accgggaggc
     4921 gacggccttc gcgatgagga gcgcggtgga gaggacgacc gcgagggtca gcagcagggc
     4981 gaacgtgttc atggcggtgg tcacttccct gcgagggcgg cacgggccgc ccggacgaga
     5041 cgggtggccc ggcccggcgg ccggggccct gagcggtcct cccaccagtg cgtcagccga
     5101 cgctgtgcga cggggtcggg gagcccgccg gagaccagga tccgctcggc gaactccatg
     5161 gcgtcgcgcc ggtaaccgcc gttcatcggg cgggacatgg cgtaggggag gaacgcggcg
     5221 cggtagccgt caccgaggat ctccggcagc tcgggggcca ccttggcgac gacgtcggcc
     5281 cgcttggcgg ccagcgccct gctctgcacc cgcagccgcc ggtggtcgaa cccctcgggg
     5341 gccggggtcc cggcgacgag cgcggagagc agtgcggtct gagcgacggc ggtacggtcg
     5401 cgggctccgg ggcgcgggat gccgggcggg agggcggtgc gcgcgagggc tccccccggc
     5461 gcgcccgcac gggcatcggc cgacggggcg gcggaggccg gcggtgcggt gagggctggc
     5521 ggtgcggtgg aggccggcgg tgcggtggag gtggaggccg gcgggtccac cgcagccgcc
     5581 gcgaccgccg ctccggtccg gggcgccggg gccaccgctg ccgccgcccg cagcgtcccc
     5641 cggatcgtcg ccagctcggc cgccagctcc gcgcccggcg ggaaggcatc gtcgcgctcc
     5701 agcaggacgc ccggcgggtc gacgcgggag cgcagctcgg ccaggacgtc caggaccggg
     5761 gcggtcaccg ggtgggcgtg cgtgtcgtgc cagacgccgt ccttctcgac cccgcccgcc
     5821 acatggacgt acgcgatggc ctccaccggc agctcgtcca gggccttcgc cgggtcctcg
     5881 ccccggttga cgtggttggt gtgcaggttg gccacgtcga tcagcagccg gaccccggtc
     5941 cgctcgacca gctccgccag gaactgcccc tcggtcagct cctcgccggg ccaggagacc
     6001 agggccgcga tgttctccag ggccagcggc acgggcagcg cgtcctgggc gatgcgtacg
     6061 ttctcgcaga gcacgtccag cgcgtcccag gtgcgcggca ccggcagcag gtggcccgcc
     6121 tccagccgcg gggaggcggt cagcggcccc ccggcccgta cgaaggcgat gtgctcggtc
     6181 accaggggcg taccgagcag ttcggcacgg gccgccagcc ccgcgagccg cccggggtcc
     6241 gggcggtcgg cgccgcccag gccgagcgag acgccgtgcg gtacgacggt gacgccccgc
     6301 tcccgcaggc ggaccaggga gtcggggagg tggtcggcgc agaggttctc cgccaccgcc
     6361 tcgacccagt cgatccccgt gagcgcctcc acctcggcgg cgatctccgg ccgccagccg
     6421 atcccgatgc ccagcctcat ccgtctcccc ctcctccgct ctgtccaggg gtgatgaccc
     6481 cgtggaacgg cggtgaaccc ggagaagggg acgttcagag gaggatttga ggttccggcc
     6541 cgcaggtcac gtcacggcat cgacgtcacg gcatcggcgg catggtcggc ggcaccggac
     6601 gggacgtgaa cgactggtcg ccggacggcg tcaccggcac cggggcgagc ggcacctgcc
     6661 cctcccgggg cgcgggcggg cccgaggggc tggcggtgga cccgcccgcg gcctcgcggg
     6721 ccagggcgtc gaagtcgacg aaaccggtgg cctccagcat ggtgatgtgg tccagcacgg
     6781 tctggttggc gtcgctggcg agctgccgga tcagggtgtt gcgggtggtg tgccggacct
     6841 ggccgatcag cccgaagacc ttgccgtggg cggcccgcag gatgttggcg aacttccgct
     6901 cgtactcgtc cccgctcgcc gccgtcagct cccgcagcca cgcctgctgc tgctcggtcg
     6961 gctggttggg cagctccacc ccgagcttgg cggccacgtc ccgggcccgc cggtccaggt
     7021 ccgtgtgccc gacgaccaga tggtccccgg cggccttcgt cgcctcgctg ggcgcccgct
     7081 cgatcgcctg ctgcccggcg ggcagctccc acagtccggc gagccgcacc ttgaccagga
     7141 agtcccggtc ggtcgcggag agcgggcccc actgggtggc gacgctggag gcgttgagat
     7201 tcgcctcgcc ggtgcccgag cggtccgcgt aggaccagac ggggaaggcg agcgcgccga
     7261 ccgtggcgac cagggccgcg atgatgaggg ccgttccgtt gatgcgtcgc aacaaggtgt
     7321 ctcccgggac gaggacgtga cagcgccgtc acaccggtcg gccaccggcc accggccgat
     7381 caccgaccgg ccgccggcca ccaaccggcg tgcggtgcag gctacttggc ggtagcgccg
     7441 ctggggcggt atcgggagat acgcggtcgg gcacggaaat actcagccgc gaccggacta
     7501 ttttcgtgcc gggtgccggg tgccgggtgc cgggtgccgg gtgccgggtg ccgggtgccg
     7561 ggtgccgggt gccgggtgcc gggtggccgg gcgggtagcg gctcgggcgc cgagagttca
     7621 ccgccgggcg ggaagcaggt cggcggccgc gcggtcagcg gcggccgggc cgtcccccgg
     7681 gcagcgggca gaaacgctcc ccgccctccg ccacgaccgt cggccccggc gcgatctcgg
     7741 tgaaccccgc gtcgcggacc accggcaggc cgctcgtgac cagctcgcgc cagcggtccg
     7801 gggcggcggt ggccacggag agcgggaagc ccgcctcgcg ccaggccttg cggtcggtct
     7861 cggacagctc ccaccaggcg agctgggcgc cgtggccgac ctgggccatg gtcttgcccg
     7921 cggacatctc cagctccggg ttgagccaga gcaccgggcc ctccgggccg ggggcggcgg
     7981 gcggttccgg gtcgtccagg tcggtgcccg agacctggag cttggccagc tccttgggcc
     8041 agccgtccag cgggatcggc gggtacaccc gtacctccgc gctcccgccc gtgaccgtga
     8101 tcccgggcag cgcggccgcc ttgcgccact ccgcgccgcg cgcccggcgc accaccttcc
     8161 ggatccgggc gtcctcccag tcccgcatgg cccgcgccca ctcgccctcg ccggtggacc
     8221 gctcgtcggc caggatcgtg agcaccgcgc gggcggcggt gcgcagggcg tccgtccggg
     8281 cgggcgggtc cgtcttctcc aggtgcagca cgagcgggag cacgtactgc ggggccgcgt
     8341 cccggtcggc ggggcgggcg gaggcggtgg ggccggcgga cgcggtggga cccgcagaac
     8401 cggtgggccc cgcagacccc gcagggcctt cggcgagggg gcccggggag atgtcgccgg
     8461 agtggctcgg ggaggtgtcg tcgctgctca ccgtcccagt ctgccaggcg gcccccgtca
     8521 cctccttggc ttaatcggcc ggtccgggtg aggatgccgg gcatgaagag cgatctgttt
     8581 tccagcgaac acctggccca gccggccacc gcccccggca tgaccctcca gaactccaag
     8641 tcgatcaagt acgccgtcaa cggggagatg catgcccgtc agggttcgat gatcgccttc
     8701 cgcggcaacc tccagttcga gcgcaagggc caggggatcg gcggcctgct caagcgcgcg
     8761 gtcaccggcg agggccttcc gctcatggcc gtacgcggtc agggcgaggc gtggttcgcg
     8821 cacgaggccg ccaactgttt catcgtggag atggaccagg gcgaccagct caccatcaac
     8881 ggccgcaacg tcctctgttt cgacgccacg ctctcgtacg agatcaggac cgtgaagggc
     8941 gccgggatgg tcggcggcgg cctcttcaac agcgtcttct ccggctacgg caagatcggc
     9001 ctgatgtgcg agggcacgcc gatcgtgatc ccggtgacgg gcgcccagcc ggtgtacgtc
     9061 gacacggacg cggtcgtcgg ctggagccag cacctcacca ccaccctgca ccgctcccag
     9121 agcgtcggct cgatggtccg cggcggctcg ggcgaggccg tccagctgat gctccagggc
     9181 gagggcttcg tcatcgtccg ccccagcgag gcccgccccg accgtacggc caactgaccc
     9241 cacggcaacc cggcggccgg cgtccgtacg ctcgtggaca tggacgacaa cgcctcgtac
     9301 tgcgagaccc ccgcctccgt ccccgcctcc gccgccccgg acggggacgc gggcccgccg
     9361 ttcgcggagt gcgtgctctg ccgtgagccg accgagtacc ccgagtcggc caagggcgcc
     9421 accctctgcc cggtctgcgc ctggcaggag gcgggacgca cggcctgctc cggctaggtc
     9481 ctgccctccc ggaccggccc tcctcccgga ccggccctcc tcccggaccg gccctcctcc
     9541 cggaccggcc ctccgtccgg accggccctc cgtccggacc ggccctccgt ctggaccgtc
     9601 cggtggcgga ccggcggacg gccgtggtcc gcaccgaggg cgtactcgaa cgtgagagcg
     9661 ccctctcctc ggacttgcgg ggcaccggcc ggcatcgcag cctgatggga ccgtgtccgc
     9721 cccgcggtcc ttcggcccgc tggtgcgcct ggatatccga ggagtgagat gtccgcgtac
     9781 ttcgagaccg caaccggtga cctcgcctcc gaagcgctgc actccttcgc cgacgtccac
     9841 gcggacctgg tgtcggccga ctcccggttc ggcttcctca cggcacgcca tccggccgca
     9901 acgcgcacgg tcggcctgct ctccggcggt ggatcggggc acgagccgct gcacggcggg
     9961 tacgtcggac ggggcatgct cgacgccgcc gtccccgggc gggtgttcgc ctccccgcac
    10021 aaccggcagg tgtacgaggc gtctctcgcg gtcgcccgcc cgggaggggt tctccacctg
    10081 gtgaagaact acaccggcga ccgcatcaac ttcgggatcg ccgccgagcg cctggcccac
    10141 aagggcatac cctgcgcccg cgtcctcatc gacgacgacc tcgcctccga cgcggagggg
    10201 atcgccgccg gacggcgcgg gacggggggc accgtcctgg tggagaagat cctcgggggt
    10261 gcggcggacc gcggcatggg gctggccgaa ctccaggacc tgggcgagcg gctcgtggcg
    10321 ggctgccgtt cactcgccgt cgccaccgcc gcccacacct cccccgccac cggcgggccc
    10381 gccttcacgc tggacgaggg gctgctggag tacggggtgg gcattcacgg ggagcgggcc
    10441 ggctcgaccg tctcccgccg cccgctggcc gagctggtcg aacgcatgac gacggaactg
    10501 ctggacgcgc ttcctgccga cgtcccggtg atatcgctgg tcaacggcct cggtgccacc
    10561 accctgctgg agctgtacgc ggcgcacggg cagctcgccc gcgtgctgcg gggcgccgga
    10621 gtgccgctcg cccgcagcct ggtcggcaac tacatcacct ccctggacat gcacggcctc
    10681 tctatcaccc tgatggccgc tgacccgacg gccctcggcc tgtacgacgc tcctgtccac
    10741 actccggccc tgcgctggtg aggaaacggt cccctgccac cgcagccgac agcccgggag
    10801 gatctctcat gaacgcaggt gccggcagtg ccgacttcac ctccggctgg atgcgccggt
    10861 tcgcggaggc ggcccacgac ggtcagcccc tgctcaccgc tctcgaccag caggtcggcg
    10921 acggggactt cggggtcaac ctgaccgccg gagtggatgc cgcccggccg cgcctggacc
    10981 ggctcccgga ccggggcgcc gatccctcgg cgccgctcct ggccacggcc gaggcgtttc
    11041 tcgacgaggt cggcggtacg agcggccccc tcttcggcct gctgctgcat tccctggccg
    11101 cggcgatcgc cgaggacggg ctcaccaccg cggccctggc cgacggcttc gcctccggcc
    11161 tcgccgcgat ccagcgtgtc ggcggtgccg ggcccgggga caagaccctc gtcgacgccc
    11221 tggcgccggc caccgacgtc ctccgctcga ccgacccgtc ggctcccgcg gctgacgcgc
    11281 tcgcggccgc ggccgcgagc gcgtgggacg gcgttgccgc caccgcgagg ctccgggccc
    11341 gcatgggacg ttccagctat ctgggcgagc gcaccgaggg gattcccgac ccgggtgccg
    11401 tcggtgtcgc cctcctgctc tcctgtgccc aggccgggag
//
