LOCUS       NZ_ARPF01000005        10870 bp    DNA     linear   CON 18-FEB-2024
DEFINITION  Streptomyces sp. SolWspMP-sol2th B083DRAFT_scaffold_4.5_C, whole
            genome shotgun sequence.
ACCESSION   NZ_ARPF01000005
VERSION     NZ_ARPF01000005.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. SolWspMP-sol2th
  ORGANISM  Streptomyces sp. SolWspMP-sol2th
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 540210)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Improved High-Quality Draft of Streptomyces sp. SolWspMP-sol2th
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 540210)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name :: Streptomyces sp. SolWspMP-sol2th
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 03:17:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,475
            CDSs (total)                      :: 6,400
            Genes (coding)                    :: 6,297
            CDSs (with protein)               :: 6,297
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 31 of 103
            Pseudo Genes (incomplete)         :: 82 of 103
            Pseudo Genes (internal stop)      :: 12 of 103
            Pseudo Genes (multiple problems)  :: 20 of 103
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:24:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 63055
            Orig. end                         :: 73925
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            ARPF01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
FEATURES             Location/Qualifiers
     region          1..10870
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10870
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10870
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5870](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5870)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(523..539)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.54"
                     /tool="antismash"
     gene            583..936
                     /locus_tag="B083_RS0112375"
     CDS             583..936
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491070.1"
                     /locus_tag="B083_RS0112375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_028417002.1"
                     /transl_table=11
                     /translation="MPPTEPAAASGRPLAHPSVAEIGLEGVLRALADPVRLRIVRGLAD
                     GHPDMACVAFDLPVSKSTTTHHFKVLREAGVIHQRYEGTSRLSRLRDADLEERFPGLLA
                     AVLEAARRTAPGA"
     gene            complement(1036..2076)
                     /locus_tag="B083_RS0112380"
     CDS             complement(1036..2076)
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-269). E-value: 3.5e-13.
                     Score: 41.3. Matches aSDomain:
                     nrpspksdomains_B083_RS0112380_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761872.1"
                     /locus_tag="B083_RS0112380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_018491071.1"
                     /sec_met_domain="ADH_N (E-value: 4.2e-29, bitscore: 95.4,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 2e-22, bitscore:
                     74.4, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVAAYAAPRAKAPLERTTIERRPVGEFDVLIEIRFAGICHSDI
                     HQARDGWGEGIFPMVPGHEIAGIVTETGPGVTRFTAGDRVGVGCMVDSCGQCDACRMGR
                     EQHCAEGNTQTYNALDRSGEPTYGGYSTHIVVRESFVVSIPEGIALDEAAPLLCAGITT
                     YSPLRRWGAGPGRKVAVIGLGGLGHMAVKIAHTLGAEVTVLSQSLRKKDDGLKLGADHY
                     YATSDPKTFEELAGTFDIILSTVSAPLDFGAYLALLRTDGTLVNVGAPEEPVSLNLFSL
                     IGGNKSLAGSAIGGIAETQEMLDFCAVHGLGAEIEVIGADQVNEAYERVLASDVRYRFV
                     IDTATI"
     aSDomain        complement(1270..2019)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B083_RS0112380_PKS_ER.1"
                     /evalue="3.50E-13"
                     /label="B083_RS0112380_PKS_ER.1"
                     /locus_tag="B083_RS0112380"
                     /protein_end="269"
                     /protein_start="19"
                     /score="41.3"
                     /tool="antismash"
                     /translation="TIERRPVGEFDVLIEIRFAGICHSDIHQARDGWGEGIFPMVPGHE
                     IAGIVTETGPGVTRFTAGDRVGVGCMVDSCGQCDACRMGREQHCAEGNTQTYNALDRSG
                     EPTYGGYSTHIVVRESFVVSIPEGIALDEAAPLLCAGITTYSPLRRWGAGPGRKVAVIG
                     LGGLGHMAVKIAHTLGAEVTVLSQSLRKKDDGLKLGADHYYATSDPKTFEELAGTFDII
                     LSTVSAPLDFGAYLALLRTDGTLVNVGA"
     misc_feature    2144..2157
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.41"
                     /tool="antismash"
     gene            2234..3181
                     /locus_tag="B083_RS0112385"
     CDS             2234..3181
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761873.1"
                     /locus_tag="B083_RS0112385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_028417003.1"
                     /transl_table=11
                     /translation="MDDQPGTSAPEPLDRRAELSEFLRTRRARLRPQDVGLPEFGRARR
                     VPGLRREELAQLAGVSVAYYTRLEQGNGRNVSAEVLDAIARALRLSDAEHAHLTHLAKP
                     GQHKKKRRPARVQRVRTGLIHLLESLEGVPAYVSGARSDILAWNPMAAAVFGDWGALPP
                     EERNWARLVFLSPGYRDLFVEWDTKASDMVSYLRLYAGCHPDDPQLSALVGELSVKNDE
                     FRRLWATHNVKEKGHGVKLLRHPLVGDMALSYETLHLPDDPEQYLVTYHAEPGSESAQA
                     LRLLASWGADAGRTEAVGGAGRAGFGGGAGRTPA"
     gene            complement(3200..3886)
                     /locus_tag="B083_RS0112390"
     CDS             complement(3200..3886)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515159.1"
                     /locus_tag="B083_RS0112390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_028417004.1"
                     /transl_table=11
                     /translation="MTKQVRAARTRQALIRSAAIVFEQHGYAQARLALISSGAGVSTGA
                     LHFHFENKAAVAQAVVAEASHGLLAVSGSIRRRTDTALQTLVDTSHALVDLLARDPVTR
                     AGFRLSCDGVRGDEPGLPTQWQRCVGELLDEAAAAGTLAEDVSRTDATAATVAATTGFE
                     VLGRYDSEWLSAPTPTGFWRLLLPRLATPETLPLLDPSGTAAATEAVATGPDGHRASGV
                     GAAAPI"
     gene            complement(4064..4924)
                     /locus_tag="B083_RS0112395"
     CDS             complement(4064..4924)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491075.1"
                     /locus_tag="B083_RS0112395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_028417005.1"
                     /transl_table=11
                     /translation="MILVTGATGAVGGEVARLLAAEGPVRILARRPERVTVHGPGVEVV
                     AGEYADREALDRALRGIDAVFLVTNSPTEPDDSRVATAARAAGVRHLVKLSMMSVEEPG
                     ADDLITRLQRGNEQAVRESGVAWTFVRPRAFMSNTLSWAAGIASDGVVRALYGDSPVAC
                     VDPRDVAAVAVAALTGTGHEGRAYAVSGPEPVTAREQTAHLSEVLGRPLRFEQLTLDRA
                     REALLAKYPRTVAEAFLESAERQRAGAKAAVVPTVEELTGRPARRFRTWASDHAESFTA
                     ADLGV"
     misc_feature    4910..4925
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.49"
                     /tool="antismash"
     gene            complement(5001..5870)
                     /locus_tag="B083_RS0112400"
     CDS             complement(5001..5870)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761876.1"
                     /locus_tag="B083_RS0112400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_106961735.1"
                     /sec_met_domain="AfsA (E-value: 3.9e-26, bitscore: 85.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELVHRSRAEDAFPRNWVRLAPDRFLVEAVLPHDHPFFAPVGADR
                     HDPLLVTEAMRQAAMLAFHAGYGVPVGHHFLMADLDYATHPEHLIVGGLPTVIDVEVTC
                     SELKWRAGLPVQGRVDWVVRRAGQLVATGMGATRFTSPRVYRRMRGGSAEPGVAIPCTV
                     PVDPARAGRDRVEDVVLSGGAREGVWELRVDTRHPTLFQRPNDHVPGMLLLEAARQAAC
                     LAAGDGRFVPAEGRTHFHRYAEFGTPCTIAADVLAGAAPGEARIRVTGRQDGEPVFDSV
                     LTGPSGTI"
     misc_feature    complement(5856..5869)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.74"
                     /tool="antismash"
     misc_feature    5954..5970
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.62"
                     /tool="antismash"
     misc_feature    complement(6012..6032)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 19.32"
                     /tool="antismash"
     gene            6105..6743
                     /locus_tag="B083_RS0112405"
     CDS             6105..6743
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606642.1"
                     /locus_tag="B083_RS0112405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_028417007.1"
                     /transl_table=11
                     /translation="MAKQDRAIRTRQAILLAAAETFEKHGYRAATITEILRIAGVTKGA
                     LYFHFPSKEELALGVLEAQEPPPPTPDQPLKLQELVDMGMMFSHRLHTSLLTRAGVRLS
                     MDQQAQGLDRTGPFLRWQQTTHDLLAHAKQNGELLPHVNPMETAGLYVAAFTGIQAVSQ
                     TLTNYRDLEQRYIALQRHILPSIATPSILTALDLTPQRTTDLARLAPAH"
     gene            complement(6779..7390)
                     /locus_tag="B083_RS0112410"
     CDS             complement(6779..7390)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459226.1"
                     /locus_tag="B083_RS0112410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_028417008.1"
                     /transl_table=11
                     /translation="MTNALTTAAAGFGTGLSLIVAIGAQNALVLRQGVRREAVLAVVAI
                     CALSDAVLITLGVAGVGAVVVAWPSALTVVSLLGALFLIGYGLLAARRAFRPSVLLPQD
                     ASSGATRGAVLACLAMTWLNPHVYLDTVFLLGSVAADRGPLRWTFGLGAVLASVCWFTA
                     LGFGARLLSGVLARPSSWRVLDGLVAAMMIGLGVRLALTA"
     misc_feature    7427..7440
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.64"
                     /tool="antismash"
     misc_feature    complement(7427..7442)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.68"
                     /tool="antismash"
     misc_feature    7434..7447
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.86"
                     /tool="antismash"
     misc_feature    7437..7450
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.42"
                     /tool="antismash"
     misc_feature    complement(7439..7452)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.51"
                     /tool="antismash"
     gene            7457..8374
                     /locus_tag="B083_RS0112415"
     CDS             7457..8374
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491079.1"
                     /locus_tag="B083_RS0112415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="WP_028417009.1"
                     /transl_table=11
                     /translation="MMTELPLDQVRTLLAVVDEGTFDAAASALRVTPSAVSQRVKALER
                     RTGRVLLMRTKPVRPTESGEVVVRFARQLARLEQDAQAALGLTGPGEPTRLTIAVNSDS
                     LATWFLPALGRVSEELGICYELRREDQDHTAALLREGLVMAAVTSSPEAVSGCSVRSLG
                     RMRYLPVATAAFAARWLGRGTATPLRELIVDAPVVDFDRRDDIQASFTRALGCGRRAAP
                     RRHFVPTSEGFVDAVAAGMGWGMLPDVHARPLLDSGRLVVLAPDRHIDVPLYWQQWKLD
                     SPALATVAEAVAAEAARALGGGGG"
     gene            8548..9210
                     /locus_tag="B083_RS0112420"
     CDS             8548..9210
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017236454.1"
                     /locus_tag="B083_RS0112420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_028417010.1"
                     /transl_table=11
                     /translation="MRVLLVEDDDDLRAVIAAGLREAGFAVDGAEDWPEADVMLHVTAY
                     DCVVLDRMLPSGDSLARLEDRRRAGWAVPVLCLTALDALDERLHGLESGADDYLAKPFV
                     MRELVLRVRSLTRRAAERLPSFLSCADVVMDLARREVRRGGVLLSLSPKEYAVLQQLLV
                     HRGSVVTRTALLEHCWDEMADPVSNVVDAVIAGVRRKLGSPPLVRTVRGQGFLLGAP"
     gene            9207..10607
                     /locus_tag="B083_RS0112425"
     CDS             9207..10607
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491081.1"
                     /locus_tag="B083_RS0112425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_028417011.1"
                     /transl_table=11
                     /translation="MNRPRGSGSARRRSPARDRLRRLQLRLTAAYTLITLAGLSCLAWL
                     VIRTDDRSREAAEYDEMRRRASVAASLVYYEDDRIRLDGLADDEATAGTPQILVLEERP
                     RGGPAVVFRGGVQQFDVPATALARAARAATRGEETVRQEARDRTGGPVRLLAVPFWHDA
                     TDEVAGAAVAVGDPAYGSAEHRSLVLSLVVGCAALTALAALTGHVLSGRSMRPAWQALE
                     QQERLLADAAHELRTPVAVMRGSVELAERRPGGAEPYLPGIRRAADRMGHVVENLLTRG
                     RLEAAVEAVRAEPLRLDQLVEEVCAELSEGGHRWELRAVESVVEADAALVRVAVRNLLD
                     NAVRHGRTAGEGAGGPLGSPERDGPAAGEPGRAGLLVTVDGPEVSVADRGPGVEPGRLP
                     ELIERFRSPGGGTGIGLSLVHRVALAHRGALTVRARPGGGAVFVLRLAPARSRRRRGSG
                     RADGPAGG"
ORIGIN
        1 gccgtcgaag gcgacggggc tgggggcgac ggcgccccag ccgccctcgc tcccggggac
       61 ggggccgtcg ccgatccagg gccgttcggt ggaggccttg cgcccggcgt gggcgatctg
      121 gatgccgggg accgtcccgt gggaggcgat cgccgcggcg atccgggcga aggcctcctg
      181 ctggcggtcg ttccacaggc ccaggtccca gggggtgatc cggccgtccg ggcgtacgcc
      241 ggtggcctcg gccatcacga gcccggcgcc cccggcggcg cgggccgcca gatgggtgag
      301 gtggaagtcg gtgggcgccc cggccgcggg cccctcggcg gccgcggagt acatgcacat
      361 cggggacatc cacacgcggt tgggaatctc cagggaccgc agggtcaggg gcgtgaacag
      421 ggcactcacg ggatgtctcc tcggggtcgg gcgcagggcc gggcgcccgg ccggccggga
      481 ggggcggggg cgccgtaccc gtacgaaatc ctcgtagtac ggtagttgtc aaactacggt
      541 gattgtcgta ctttcaggcc cgtcggacct ccaggaggct ccatgccccc caccgaaccc
      601 gccgccgcgt ccgggcgccc gctcgcccat ccgtccgtgg ccgagatcgg cctggagggg
      661 gtcctgcggg cgctggccga tcccgtccgg ctgcggatcg tccgggggct cgcggacggg
      721 cacccggaca tggcgtgcgt cgcgttcgac ctcccggtga gcaagtcgac caccacccac
      781 cacttcaagg tgctgcgcga ggcgggcgtc atccaccagc gctacgaggg gacctcccgc
      841 ctgagccgac tgcgcgacgc cgacctggag gagcgtttcc ccgggctgct ggcggccgtg
      901 ctggaggcgg cgcggcggac cgcccccggc gcctgagcgt gcgggggtgc cccgggcccg
      961 gtgaggaccc ggggcacccc cgcgcacctg tgcgggaacc ctggacggcg cacccgcgta
     1021 cggctgccgg accggtcaga tggtcgccgt gtcgatcacg aagcggtagc ggacgtcgct
     1081 ggcgagaacg cgctcgtacg cctcgttgac ctggtccgcg ccgatcacct cgatctcggc
     1141 gccgaggccg tgcacggcgc agaagtcgag catctcctgc gtctccgcga tgccgccgat
     1201 cgccgagccc gccagcgact tgttgccgcc gatgagcgag aagaggttga gcgagaccgg
     1261 ctcctcgggc gcgcccacgt tgacgagggt gccgtccgtg cgcagcaggg cgaggtaggc
     1321 gccgaagtcc agcggggcgg agaccgtgga gaggatgatg tcgaaggttc cggccagctc
     1381 ctcgaaggtc ttcgggtcgc tggtcgcgta gtagtggtcg gcgcccagct tgagcccgtc
     1441 gtccttcttg cgcagggact gggagaggac cgtgacctcg gcgccgaggg tgtgcgcgat
     1501 cttgacggcc atgtggccga gacccccgag gccgatgacg gcgaccttcc tgccggggcc
     1561 ggcgccccag cggcggagcg gggagtaggt ggtgatgccc gcgcagagca gcggcgcggc
     1621 ctcgtccagg gcgatgccct cggggatgga gacgacgaag ctctcgcgga cgacgatgtg
     1681 cgtcgagtag cccccgtacg tcggctcgcc gctgcggtcg agggcgttgt acgtctgggt
     1741 gttcccctcg gcgcagtgct gttcgcggcc catgcggcag gcgtcgcact gtccgcagga
     1801 gtcgaccatg cagccgacgc cgacgcggtc gccggccgtg aagcgggtga cgccgggccc
     1861 ggtctcggtg acgatcccgg cgatctcgtg gccgggcacc atcgggaaga tgccctcgcc
     1921 ccagccgtcc cgggcctggt ggatgtcgga gtggcagatg cccgcgaacc tgatctcgat
     1981 gaggacgtcg aactcgccca cggggcggcg ctcgatcgtg gtgcgctcca gcggggcctt
     2041 ggcacgggga gcggcgtagg cggcaacggt ggtcatgccg gaggttctcc tctgggtgat
     2101 gcggtaggaa cgaacgtcat ccagcatcac gggacccgca gcgtttaccc agatcaccac
     2161 cttgcctacg tccgccggtc ctactcctgg cagggacagg ctcctgtgcg tacgaccagg
     2221 aataatggga gccatggacg atcagccagg cacctccgcc cccgagccgc tcgaccggcg
     2281 cgccgaactc agcgagttcc tgcgcacccg gcgggcccgg ctccggccgc aggacgtggg
     2341 gctgccggag ttcggacggg cccgccgggt gccggggctg cggcgggagg agctggcgca
     2401 gctggccggg gtttccgtgg cgtactacac ccggctggaa cagggcaacg ggcgcaacgt
     2461 ctcggccgag gtgctcgacg cgatcgcccg ggcgctgcgg ctcagcgacg ccgagcacgc
     2521 gcatctgacc catctggcca agcccgggca gcacaagaag aagcgccgtc cggcccgggt
     2581 gcagcgggtg cggaccgggc tgatccatct cctggagagc ctggagggcg ttcccgcgta
     2641 cgtcagcggg gcgcgctccg acatcctggc ctggaacccg atggcggcgg cggtcttcgg
     2701 cgactggggc gcgctgccgc ccgaggagcg caactgggcg cggctcgtgt tcctctcccc
     2761 cggctaccgg gacctgttcg tggagtggga caccaaggcc tcggacatgg tcagctatct
     2821 gcgcctgtac gcggggtgcc acccggacga tccgcagctc tccgcgctgg tgggtgaact
     2881 ctccgtcaag aacgacgagt tcaggcggct gtgggccacg cacaacgtca aggagaaggg
     2941 ccacggcgtc aagctcctgc gccatccgct cgtcggggac atggccctct cctacgagac
     3001 cctgcacctg ccggacgacc ccgagcagta tctggtcacc taccacgcgg agccgggctc
     3061 ggagtcggcc caggcgctgc gcctgctggc gagctggggc gcggacgcgg gacggaccga
     3121 ggcggtcggc ggggccggcc gggccgggtt cggcggcggg gcgggccgga ctccggcctg
     3181 aacctccgcc gccgcacggt cagatggggg cggcggcccc caccccggaa gcgcggtggc
     3241 cgtccgggcc cgtggcgacg gcctccgtcg ccgcggcggt gcccgagggg tccaggaggg
     3301 gcagggtctc cggggtggcg aggcggggca gcaggagccg ccagaagccg gtgggggtgg
     3361 gggcggagag ccactcggag tcgtaccgtc cgagcacctc gaacccggtg gtcgcggcca
     3421 ccgtggcggc tgtggcgtcc gtacgggaga cgtcctcggc cagggtgccg gcggcggccg
     3481 cctcgtccag caactcgcct acgcagcgct gccattgggt gggcaggccc ggttcgtcgc
     3541 cccgtacccc gtcacagctg agccggaagc cggcgcgggt gaccgggtcg cgggcgagga
     3601 ggtcgaccag ggcgtgggag gtgtcgacca gggtctgcag ggccgtgtcc gtccggcggc
     3661 ggatggagcc ggacaccgcc agcaggccgt gcgaggcctc cgccaccacg gcctgggcga
     3721 ccgcggcctt gttctcgaag tggaagtgca gcgcacccgt gctgaccccc gcgccggaac
     3781 tgatcagcgc caggcgcgcc tgcgcgtacc cgtgctgctc gaacacgatc gcggcggagc
     3841 ggatcagggc ttggcgggta cgcgcggcac gcacctgctt ggtcaccgga gcctccgccg
     3901 gggggagaag cggatgggaa gggggcctgg gccaggggcc gcgagggccg ggcactctcc
     3961 gccggagcgg gcaagcagcg tcatgagaat atcaaaccaa cttaccggtt tgatttccag
     4021 gggaacgtgt gcgcggtcgg ccaccgtgcg ggcgcgcgtt ccgctagacg cccaggtcgg
     4081 ctgcggtgaa ggactccgcg tgatccgacg cccaggtacg gaatctcctg gcgggacggc
     4141 ccgtcagctc ctcgacggtg ggcaccacgg ccgccttggc tccggcgcgc tggcgctcgg
     4201 cgctctccag gaacgcctcg gccacggtgc gcggatattt cgcgagcagg gcctcgcgcg
     4261 cccggtccag ggtcagttgc tcgaagcgca gcggccggcc cagcacctcc gagaggtggg
     4321 ccgtctgctc gcgagcggtg acgggctccg ggccggacac cgcgtacgcc cgcccctcgt
     4381 ggcccgtgcc ggtgagggcg gcgaccgcca ccgccgcgac atcccgcggg tcgacgcacg
     4441 cgaccgggga gtcgccgtac agggctcgca ccacgccgtc cgacgcgatg cccgcggccc
     4501 aggagagcgt gttggacatg aaggccctgg ggcggacgaa cgtccaggca acccccgatt
     4561 cccgtacggc ctgttcgttg ccccgctgga gacgggtgat caggtcgtcg gcgccgggct
     4621 cctccaccga catcatcgac agcttcacca gatggcgcac gcccgccgcg cgcgcggccg
     4681 tcgcgaccct cgagtcgtcc ggctccgtgg ggctgttggt caccaggaac accgcgtcga
     4741 tcccccgtag cgcccggtcg agcgcctcac ggtccgcgta ctcccccgcc acgacctcca
     4801 caccggggcc gtgcaccgtc acgcgttccg gccgcctcgc caggatccgt accgggccct
     4861 cggccgccag cagtcgcgcg acctcaccgc cgaccgcccc ggtcgcccct gtcaccagaa
     4921 tcacgtcacg tactcctcat gaaccgtcag cgggccgccg cagggccgcc cgtcgccacc
     4981 gcgccgtccg gacgcgtgcc tcagatggtg ccggacgggc ccgtcagcac cgaatcgaag
     5041 accggctcgc cgtcctgacg acccgtcacc cggatcctgg cctcgcccgg cgcggcgccc
     5101 gccaggacgt ccgcggcgat cgtgcacggg gtaccgaact ccgcgtaccg gtggaagtgc
     5161 gtacgcccct cggcggggac gaacctgccg tccccggcgg ccaggcacgc cgcctgacgg
     5221 gccgcctcca gcagcagcat gcccggcaca tggtcgttgg ggcgctggaa gagcgtcggg
     5281 tggcgagtgt ccacccgcag ctcccagacc ccttcgcgcg cgccccccga cagcaccacg
     5341 tcctcaaccc tgtcgcggcc cgcccgggcg gggtcgaccg gcaccgtgca cggtatcgcc
     5401 acgccaggct ccgccgatcc gccgcgcatc cgccggtaga cccgcgggct ggtgaacctg
     5461 gtcgcgccca tccccgtcgc gaccagctgc ccggcgcggc gcaccaccca gtcgacccgg
     5521 ccctgcaccg gcagcccggc acgccacttc agctcggagc aggtcacctc gacgtcgatc
     5581 acggtgggca gaccgcccac gatcaggtgc tcggggtggg tcgcgtagtc cagatccgcc
     5641 atcaggaagt ggtggccgac ggggactccg tacccggcgt ggaaggccag catcgcggcc
     5701 tgccgcatcg cctccgtcac cagcagcggg tcatggcggt ccgccccgac cggggcgaag
     5761 aaggggtggt cgtgcggcag caccgcctcc acgaggaacc ggtcgggggc cagccgcacc
     5821 cagttgcgcg ggaacgcgtc ctccgccctg ctccggtgga ccagttccat gggcaccgga
     5881 tgcgccactc ggggctccgg acccacggct gacgccgtta caggatcggt caaaactgct
     5941 gcttcgggca taactccccc caggggttat gtgatgggcg agccgttcct gcggtgcaca
     6001 cgcgctgata agatacagac taagcggttt tttttctatc cccaggaccc ccacaaaccg
     6061 cccgcccggc ccacgtccgg cacagcccca cgaaggagac agggatggcc aagcaggacc
     6121 gggcgatccg cacgcgccag gcgatcctgc tggccgcggc ggagactttc gagaaacacg
     6181 ggtaccgggc cgcgaccatc acggaaatac tgagaatcgc cggggtgacg aaaggcgcgc
     6241 tctatttcca tttcccgtcc aaggaggaac tcgcgctcgg ggtgctggag gcgcaggagc
     6301 ccccgccgcc cacccccgac cagccgctca aactccagga actggtggac atggggatga
     6361 tgttcagcca ccgcctccac accagcctcc tcacccgcgc cggggtccgg ctctccatgg
     6421 accagcaggc ccaggggctg gaccggaccg gtccgttcct ccgctggcag cagaccaccc
     6481 acgacctcct ggcccacgcc aaacagaacg gcgaactcct cccgcacgtc aaccccatgg
     6541 agacagccgg cctctacgtc gcagcgttca ccggcatcca ggcggtctcc cagaccctga
     6601 ccaactaccg cgatctggaa cagcgttaca tcgcactcca gcggcacatc ctccccagta
     6661 tcgccacacc ctccatcctc accgcactcg acctcacccc ccagcgcacc accgacctcg
     6721 cccggctcgc gcccgcgcac tgaccgcccg accgtccgga ggccgggcgg tcaggcggtc
     6781 aggcggtcag ggccaggcgg acgccgaggc cgatcatcat cgcggcgacc agcccgtcca
     6841 gcacccgcca cgacgacggg cgggccagca ccccgctcag cagccgggcg ccgaagccga
     6901 gggcggtgaa ccagcagacg ctggcgagga ccgcgcccag cccgaaggtc cagcgcagcg
     6961 gcccgcggtc ggcggcgacg gagcccagca ggaagacggt gtccaggtag acgtgcggat
     7021 tgagccaggt catcgcgagg caggcgagca cggcaccccg cgtcgccccg gaggacgcgt
     7081 cctgcggcag gaggacggag gggcggaagg cccggcgcgc ggccaggagg ccgtagccga
     7141 tcaggaacaa cgcgcccagc agggagacca ccgtcagggc ggagggccag gccacgacga
     7201 cggccccaac cccggcgaca cccagggtga tgaggaccgc gtcggacagc gcgcagatgg
     7261 cgaccaccgc gagaaccgcc tcccggcgga cgccctggcg caggaccagg gcgttctggg
     7321 cgccgatggc gacgatgagg gagaggccgg taccgaaccc ggcggccgcg gtggtcagag
     7381 cgttggtcat ggcccagacg ttagagagcc tcgacacccc tgtacagcta aagattctta
     7441 actatcatta gctgccgtga tgacggaact ccccctcgat caggtgcgga ccctcctggc
     7501 cgtcgtcgac gaaggcacct tcgacgccgc ggcgagcgcc ctgcgggtga cgccgtcggc
     7561 cgtcagccag cgggtgaagg ccctggagcg gcgcaccggc cgggtgctgc tgatgcggac
     7621 gaagccggtg cggcccaccg agtccggcga ggtcgtggtc cgcttcgccc ggcagctggc
     7681 ccggctggag caggacgcgc aggcggccct cgggttgacc ggccccggcg agccgacccg
     7741 gctgacgatc gcggtcaact ccgactcgct ggccacctgg ttcctgcccg ccctcggccg
     7801 ggtgtcggag gagctgggca tctgctacga gctgcgccgg gaggaccagg accacaccgc
     7861 cgcgctgctc cgcgaggggc tggtgatggc cgcggtgacc tcgtccccgg aggcggtgag
     7921 cgggtgctcg gtgcggagtc tggggaggat gcgctatctg ccggtggcga cggctgcctt
     7981 cgcggcgcgg tggctcgggc gggggacggc gaccccgctg cgcgagctga tcgtggacgc
     8041 gccggtggtg gacttcgacc ggcgcgacga catccaggcc tccttcaccc gggccctcgg
     8101 atgcggacgg agggcagccc cgcgccgtca cttcgtcccc acctcggagg ggttcgtgga
     8161 cgccgtggcg gcggggatgg ggtgggggat gctgcccgac gtccacgccc gcccgctgct
     8221 ggactccgga cggctggtcg tcctcgcccc ggaccggcac atcgacgtcc ctctgtactg
     8281 gcagcagtgg aagctcgact caccggccct ggcgaccgtg gccgaggccg tggcggcgga
     8341 ggccgcacga gcgctcggcg gtggcggcgg ctgaacgagg gcgggcggcg ggcgacgggg
     8401 aacgcgcgca agcacgggcc ggggccggcc gggaaggcag ccggggcggg ccacgcctta
     8461 ggatggcggg tcttccccca ggtcgggctg ccgttgccgg tacggcggcg gtgcggggcc
     8521 ggccccgagc gacccggagg tgtctccttg cgcgtgctgc tggtggagga cgacgacgac
     8581 ctgcgggcgg tgatcgccgc cgggctgcgc gaggccgggt tcgccgtgga cggggcggag
     8641 gactggccgg aggcggacgt gatgctccat gtcaccgcgt acgactgcgt ggtcctggac
     8701 cgcatgctgc cctccggcga ctccctcgcg cggctggagg accggcgccg ggcggggtgg
     8761 gcggtgcccg tgctctgcct caccgccctg gacgctctgg acgagcggct gcacgggctg
     8821 gagagcgggg ccgacgacta cctcgccaaa cccttcgtga tgcgggaact cgtcctgcgc
     8881 gtacggagtc tgacccggcg ggcggccgag cggctgccct ccttcctgag ctgcgccgac
     8941 gtggtgatgg acctggcccg gcgcgaggtg cggcggggcg gggtgctgct ctcgctgagc
     9001 ccgaaggagt acgccgtgct ccagcagctc ctggtccacc ggggcagcgt cgtcacccgt
     9061 acggctctgc tggagcactg ctgggacgag atggccgacc cggtctccaa cgtggtggac
     9121 gccgtcatcg ccggggtgcg gcgcaagctg ggctccccgc ccctcgtacg gaccgtgcgc
     9181 gggcaggggt tcctgctggg cgccccgtga accggccgcg gggctccggg tcggcacggc
     9241 gccgctcccc cgcccgggac cggctgcgcc ggctccagct gcgcctgacc gccgcgtaca
     9301 cgctgatcac cctcgccgga ctcagctgcc tggcctggct ggtgatccgt accgacgacc
     9361 ggtcgcggga ggccgccgag tacgacgaga tgcgccgccg ggcctccgtc gcggcctcgc
     9421 tcgtctacta cgaggacgac cgcatccggc tcgacggcct ggccgacgac gaggcgacgg
     9481 cgggcacgcc ccagatcctg gtgctggagg aacggccccg gggcggcccg gccgtggtgt
     9541 tccggggcgg cgtccagcag ttcgacgtac cggccacggc actcgcccgg gcggcgcgcg
     9601 cggcgacgcg cggggaggag accgtgcggc aggaggcccg cgaccgtacg ggcgggcccg
     9661 tccggctgct ggcggtgccc ttctggcacg acgcgaccga cgaggtggcg ggcgcggccg
     9721 tcgcggtcgg ggacccggcg tacgggtcgg ccgagcaccg cagcctggtc ctctccctgg
     9781 tcgtcgggtg cgcggccctc accgccctgg ccgcgctcac cggccatgtg ctctccgggc
     9841 ggagcatgcg gcccgcctgg caggcgctgg agcagcagga gcggctgctc gccgacgcgg
     9901 cgcacgagct gcggacgccg gtggcggtga tgcgcggttc ggtggagctg gccgagcgcc
     9961 gtcccggcgg ggcggaaccg tatctgccgg ggatccgccg ggcggccgac cggatgggcc
    10021 atgtggtgga gaacctgctg acccggggcc ggctggaggc cgccgtggag gcggtacggg
    10081 ccgagccgct gcgcctggac cagctcgtgg aggaggtctg cgccgagctg tcggagggcg
    10141 ggcaccggtg ggagctgcgg gcggtggagt cggtggtcga ggccgacgcg gcgctggtcc
    10201 gggtggcggt gcgcaacctg ctcgacaacg ccgtgcggca cggccggacg gccggggagg
    10261 gggcgggcgg gccgctcggc agccccgaac gcgacggtcc cgcggccggg gagcccggcc
    10321 gggccggtct cctggtgacc gtcgacgggc cggaggtgtc ggtggccgac cgggggccgg
    10381 gggtggagcc gggccggctg ccggagctga tcgagcgctt ccgttcgccg ggcggcggta
    10441 cgggcatcgg gctctcgctg gtgcaccggg tggccctcgc ccaccggggc gctctgaccg
    10501 tccgcgcccg gcccggtggc ggtgcggtgt tcgtgctgcg gctcgcgccc gcccgctcgc
    10561 ggcggaggag gggttcgggg cgggcggacg gaccggccgg agggtgacgc tcacgattcg
    10621 ctcatgatcg gccgtccatg atgcaggggc aggtacgcag accacgcccg ggagtcccca
    10681 tgtccaagca cgccgccacc cccttcctcc gccgcaggtc gaccctcgcg gccctggccg
    10741 gcggggtcgc cgtggcggcc ggcgcggggg tcctgctctc cagcgccttc gcggccaggc
    10801 ccgcgccgga ggtcgtcctc ccgcaggcga acgccacctt cgactaccag atcggcgggg
    10861 cctacacccc
//
