LOCUS       NZ_ARPF01000002        15248 bp    DNA     linear   CON 18-FEB-2024
DEFINITION  Streptomyces sp. SolWspMP-sol2th B083DRAFT_scaffold_1.2_C, whole
            genome shotgun sequence.
ACCESSION   NZ_ARPF01000002
VERSION     NZ_ARPF01000002.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sp. SolWspMP-sol2th
  ORGANISM  Streptomyces sp. SolWspMP-sol2th
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 720914)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Improved High-Quality Draft of Streptomyces sp. SolWspMP-sol2th
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 720914)
  AUTHORS   Currie,C., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name :: Streptomyces sp. SolWspMP-sol2th
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: Missing
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            Funding Program                   :: DOE-GLBRC 2011
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 03:17:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,475
            CDSs (total)                      :: 6,400
            Genes (coding)                    :: 6,297
            CDSs (with protein)               :: 6,297
            Genes (RNA)                       :: 75
            rRNAs                             :: 6, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 31 of 103
            Pseudo Genes (incomplete)         :: 82 of 103
            Pseudo Genes (internal stop)      :: 12 of 103
            Pseudo Genes (multiple problems)  :: 20 of 103
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:24:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 507743
            Orig. end                         :: 522991
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            ARPF01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
FEATURES             Location/Qualifiers
     region          1..15248
                     /candidate_cluster_numbers="1"
                     /candidate_cluster_numbers="2"
                     /candidate_cluster_numbers="3"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /product="ectoine"
                     /region_number="2"
                     /rules="AfsA"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..15248
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /detection_rules="ectoine_synt"
                     /kind="neighbouring"
                     /product="butyrolactone"
                     /product="ectoine"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     cand_cluster    1..10882
                     /candidate_cluster_number="2"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10882
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5882](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5882
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(556..569)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.74"
                     /tool="antismash"
     misc_feature    623..636
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.22"
                     /tool="antismash"
     gene            674..1153
                     /locus_tag="B083_RS0105845"
     CDS             674..1153
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1240: riboflavin biosynthesis protein RibD"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763177.1"
                     /locus_tag="B083_RS0105845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleoside deaminase"
                     /protein_id="WP_018489557.1"
                     /transl_table=11
                     /translation="MGTHDSELMGFAHEAIRLSREHVAQGGIPFSGVVVGGGRILGTGF
                     NRVREDNDPTAHAEVVALREATTRHGIRAVAGATLIASGEPCALCYMAARFAGIGHIVH
                     TADRRTAARYGFDYTSTYSLFAEDPERWPIKVTALQLPEAEAPFQDFLTMSRTRH"
     gene            1270..1590
                     /locus_tag="B083_RS0105850"
     CDS             1270..1590
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967532.1"
                     /locus_tag="B083_RS0105850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="multidrug efflux SMR transporter"
                     /protein_id="WP_018489556.1"
                     /transl_table=11
                     /translation="MHWLYLAIAVAFEIAVAIAAGKAEGFKNRKWTGITLASGAAATFF
                     LSKALLTFDVGVGYALWTSVAGVGITILGALFFGQRLNWNKAIGIVLVIGGVVGLQLSG
                     SA"
     gene            1672..2088
                     /locus_tag="B083_RS0105855"
     CDS             1672..2088
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763175.1"
                     /locus_tag="B083_RS0105855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="multidrug efflux SMR transporter"
                     /protein_id="WP_019763175.1"
                     /transl_table=11
                     /translation="MSNAVKNPTNAWLSLLLAGVFEIGYALAVGGSEGFTVLTWSLVAV
                     VFFLLTLYALSLALRTIEVSIGYAVWAGIGAVGAAILGPVFFDETLTLAKGIWLTVIIV
                     GVIWLKLADRTKPEAVAPAPAAQQVTDRPGALQV"
     gene            complement(2241..3077)
                     /locus_tag="B083_RS0105860"
     CDS             complement(2241..3077)
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (33-203). E-value: 2.8e-14.
                     Score: 45.3. Matches aSDomain:
                     nrpspksdomains_B083_RS0105860_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489554.1"
                     /locus_tag="B083_RS0105860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_232790292.1"
                     /sec_met_domain="adh_short (E-value: 1.5e-38, bitscore:
                     127.8, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 3.4e-32, bitscore:
                     107.6, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPGGGPQPGRAAAGGEPPSGRAAAGGGLLSGKVALVTGASSGIG
                     AAAARHFADEGAAVVLVARRAALLEEGAERIRKDGGRAVAVPGDVTRPADMERAVATAV
                     ERFGRLDCAFNNAGHASAGTLLHELPDDAFDRTLDVNLRGVWNCLRAQLPALMAAGGGA
                     VVNTSSVAGVRATAASAAYVAAKHAVIGLTRAAAAEYGEHGIRVNALVVGGTDTEMMDA
                     LRPPGPAVGAGFGSQGIQRRLADPVEIARAAAWLCSDASSFTTGTALAVDGGRTAK"
     aSDomain        complement(2469..2978)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B083_RS0105860_PKS_KR.1"
                     /evalue="2.80E-14"
                     /label="B083_RS0105860_PKS_KR.1"
                     /locus_tag="B083_RS0105860"
                     /protein_end="203"
                     /protein_start="33"
                     /score="45.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VALVTGASSGIGAAAARHFADEGAAVVLVARRAALLEEGAERIRK
                     DGGRAVAVPGDVTRPADMERAVATAVERFGRLDCAFNNAGHASAGTLLHELPDDAFDRT
                     LDVNLRGVWNCLRAQLPALMAAGGGAVVNTSSVAGVRATAASAAYVAAKHAVIGLTRAA
                     AAEYGEH"
     CDS_motif       complement(2883..2972)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B083_RS0105860_0001"
                     /evalue="9.10E-07"
                     /label="PKSI-KR_m1"
                     /locus_tag="B083_RS0105860"
                     /protein_end="65"
                     /protein_start="35"
                     /score="21.5"
                     /tool="antismash"
                     /translation="LVTGASSGIGAAAARHFADEGAAVVLVARR"
     gene            complement(3074..3820)
                     /locus_tag="B083_RS0105865"
     CDS             complement(3074..3820)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763173.1"
                     /locus_tag="B083_RS0105865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_028416019.1"
                     /transl_table=11
                     /translation="MLSLRLAALNIDGVLLNDTFSPVIHQFVTSRGGTYSAALERSVFS
                     QPRHIAGRRLAAAVGGGMTGEAALAAYFEERAAHVDRHPVMITPGTRQLLHRLRSAGLR
                     TVCYGGLGKEHFDHHLGRYAHLFDGPGYVCTNDFRPGLREITEDVFGLRPAQALFVDDV
                     ARVADAARELGTAFVGRPSAFAHSHQRALMREAGVRHLIDRLDTLDGGLLRRIDQEMNA
                     DTFWSAPLTAAGPGGYASASGRGGYA"
     misc_feature    complement(3809..3811)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    3902..3916
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     gene            complement(4031..4663)
                     /locus_tag="B083_RS0105870"
     CDS             complement(4031..4663)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452959.1"
                     /locus_tag="B083_RS0105870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_018489552.1"
                     /transl_table=11
                     /translation="MAQPKQERAEQTRRALLHAAAEVFDEYGYAGASITRILKRAGVTA
                     GALYFHFGSKEDLAKAVMNSQPETLVPLVAAGGLQRLIDLTLVWSWQLQRDPLLRAGVR
                     LTNEQTSTEGPANADPYEKFRAIMTGYLEEAQEDGELQPGVDPAVVAEFVVAACTGMQM
                     YSNVVSGRRDLPERTQQMWNLILPGIAVPSVITRAEATPARGAAMMP"
     misc_feature    complement(4679..4694)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.84"
                     /tool="antismash"
     misc_feature    4745..4763
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.07"
                     /tool="antismash"
     misc_feature    complement(4804..4821)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.91"
                     /tool="antismash"
     cand_cluster    4874..15248
                     /candidate_cluster_number="3"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    4874..15248
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[9873:10248](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      complement(9874..10248)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="2"
     gene            5001..5882
                     /locus_tag="B083_RS0105875"
     CDS             5001..5882
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489551.1"
                     /locus_tag="B083_RS0105875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_269801819.1"
                     /sec_met_domain="AfsA (E-value: 2e-24, bitscore: 80.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHQWQVHKLRGEEVLLSTWAEPCPGRFVITARWPKGHAFYRPSQG
                     AYDPLLLAETVRQAVPLLSHAVHGVPREHRQAWEYVSFAVEPLASLVTTAEDEVRLVVT
                     CSNVVRRGTRFAGMTMDIDVHLGKRLMGIAHTRFNNQPEAIYRRLRGSYADIHTALEHC
                     LPTGPPVEPQRVGRCSPADVVLSPALGGERPGRWLLRVDPAHPVLFDHPVDHVPGMLLL
                     EAARQAAHAVDGTAGAAVIGMRTDFVRYVELDAPAVVVAGSPVPAGTGRRRVPVSVEQH
                     GVRVFTSEVTLA"
     gene            complement(5973..6719)
                     /locus_tag="B083_RS0105880"
     CDS             complement(5973..6719)
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: ACPS (132-191). E-value: 6.1e-09. Score:
                     27.7. Matches aSDomain:
                     nrpspksdomains_B083_RS0105880_ACPS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1012: 4'-phosphopantetheinyl transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763171.1"
                     /locus_tag="B083_RS0105880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="WP_028416021.1"
                     /transl_table=11
                     /translation="MTHDLKWSDAPPGEIRDQVAVWIVRAPAGQRLPPDGSPPDWLDEK
                     ERSRLAAIPQHSGKEAYAFAHWALRSVLGSSLGCPPRSVGFLRQPCPGCGGVHGRPAIR
                     DPDRGGEPPEFSMSHSGDHVAIAVAEATIGIDIESHPTPESVAGYLPFLHPREQQWLGG
                     RPDDELVRDFARLWTRKEAMAKATGQGMAAVMNRLDLTGQPLGWRVRQLIAPPGYEASF
                     AVPASVHLEVVMHDELPYPMPELEQV"
     aSDomain        complement(6147..6323)
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B083_RS0105880_ACPS.1"
                     /evalue="6.10E-09"
                     /label="B083_RS0105880_ACPS.1"
                     /locus_tag="B083_RS0105880"
                     /protein_end="191"
                     /protein_start="132"
                     /score="27.7"
                     /tool="antismash"
                     /translation="IGIDIESHPTPESVAGYLPFLHPREQQWLGGRPDDELVRDFARLW
                     TRKEAMAKATGQGM"
     gene            complement(6775..7017)
                     /locus_tag="B083_RS0105885"
     CDS             complement(6775..7017)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489549.1"
                     /locus_tag="B083_RS0105885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6253 family protein"
                     /protein_id="WP_018489549.1"
                     /transl_table=11
                     /translation="MSLLPAYGYQAAVQETCDTPEYHMPLVCWHDDANGVRGLVLYRGA
                     LRPAEQVPGFRRYLAAEGLEQYATTGPWPLLAATA"
     gene            complement(7014..7829)
                     /locus_tag="B083_RS0105890"
     CDS             complement(7014..7829)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763169.1"
                     /locus_tag="B083_RS0105890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="maleate cis-trans isomerase"
                     /protein_id="WP_018489548.1"
                     /transl_table=11
                     /translation="MTQFQAREDGWGAVARVGVVVPHADVGPESELQAMAPRDVYIHGS
                     RVHFGAMRPGGEMDPKIPHDPVRAFIDPPFIDDAVELLAASPLDSIALGFTSSAYVLGA
                     DGEQKLFERLAERTRGIPLTGTTHAAAAGFRALGAERIALVNPPWFDDELSALGASYFG
                     ERGFDVVHHAPCGLPSNQKLITPEALVQWIESTVAPQRPQAVLVAGNGIRAVGTIAGLE
                     ERIGLPVLTANQVLFWHALHLAGGTGAAARISDYGALFGVRPEPGSVTG"
     gene            complement(7916..8635)
                     /locus_tag="B083_RS0105895"
     CDS             complement(7916..8635)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489547.1"
                     /locus_tag="B083_RS0105895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transglutaminase family protein"
                     /protein_id="WP_028416022.1"
                     /transl_table=11
                     /translation="MTDTLTDAVPAPPAAEDLDRLTAATDFLDHDHETVRAFVDKALQG
                     VDRDTATPVELAVALYYAVRDGIHYEVYGADLSPQGLKASSIITGGKGFCLHKSVLYAA
                     ACRSVGIPARLHYSDVRNHLASDRLRSYIGGDVFFHGLATVYLEGRWLQVTPVFNKLLC
                     RLYGMTPLEFDGRSDSLYHPFDAQGRQSMEFLTDHGDFDDVPYEFVMGNMRRKHPKFLD
                     AEGTGTVSGGSLADEAS"
     gene            complement(8651..9781)
                     /locus_tag="B083_RS0105900"
     CDS             complement(8651..9781)
                     /GO_function="GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489546.1"
                     /locus_tag="B083_RS0105900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_028416023.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 2.3e-18, bitscore:
                     62.2, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 5.8e-27, bitscore:
                     89.1, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 7.8e-34, bitscore:
                     112.3, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAEKDTDTYGITAPYGDELNDDVARWDRAGEFPQEKWKPLQRSG
                     LFTLPFAPEYGGAGRTLTETMAALEGLGHTSRDAGLNFSASTQIVSVGIPLQAFGSEEL
                     RSRHLPRVTSGEAITAHAITEPGHGSDVMNIRTTAVREGDEYVIDGGKMFITNAPVADL
                     FLLYVRTGKPGAFGLSTFLAERNTPGLRIGEPLETMGLRTSPISEVTLDNVRLPVANRL
                     GSEGAGFLIMDYVMKREVLFSFAVNLGEMTHRLKRVVEHATTRQQFGTAIGKNQAVSHK
                     IADMRIAVETARKWLYDTGRKVEQGTDASLDVAATKIVVSEANQRTAQHAIQIFGARGY
                     LTDTGIERELRNATSGSIYSGTNEIQRNCIAALMGL"
     gene            complement(9874..10248)
                     /locus_tag="B083_RS0105905"
     CDS             complement(9874..10248)
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452967.1"
                     /locus_tag="B083_RS0105905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_018489545.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.5e-25, bitscore:
                     85.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLSDVPAVEWGSGTSHRLLTEADGMGFTVCETLVRRGTTSG
                     LQYLGHLEACYCIGGSGEVVTTDGTRHKIKTGTIYALDQHDAHHLIASDDEDLLLVSVF
                     SPALRGDEKHSLSEDGFSRY"
     gene            complement(10445..10705)
                     /locus_tag="B083_RS0105910"
     CDS             complement(10445..10705)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763164.1"
                     /locus_tag="B083_RS0105910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl carrier protein"
                     /protein_id="WP_028416024.1"
                     /sec_met_domain="PP-binding (E-value: 5.6e-06, bitscore:
                     22.1, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTGAHDPIRAYIVNEFLAGEDAEDLTDDFDLIESTVVNSLGLVR
                     LISWISETYHIPVDDIPLEPAAYRTLADIRGFVAQAAPASV"
     gene            complement(10702..12150)
                     /locus_tag="B083_RS0105915"
     CDS             complement(10702..12150)
                     /NRPS_PKS="Domain: AMP-binding (1-382). E-value: 2e-58.
                     Score: 189.9. Matches aSDomain:
                     nrpspksdomains_B083_RS0105915_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489543.1"
                     /locus_tag="B083_RS0105915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AMP-binding protein"
                     /protein_id="WP_028416025.1"
                     /sec_met_domain="AMP-binding (E-value: 1.2e-59, bitscore:
                     197.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQEQPDSTALVASGERVTYAELERLTRLAAADLDGAGFGFVRALC
                     VPAHKSPETIALLLAAWRQGINTLVPSPSLGAAALATLTAQAHGPLTATAVPGGGVVLA
                     REETDPALADGFVVPDPAESLLTLTTSGSTGVPKLVPIRTEAFDRFADWATGKFGLDGS
                     TRALSFSPLNFDLALLDVWTVLEAGGTVVLADAGASGDAGYLRELALAHEVTLVQGVPL
                     LHRLLAADGARYPAVRTVVFTGEAVSEQGLRDAFAACPAARFHNVFGCTETNDSFIEDV
                     DPDAYVHPLPIGTPIDGTDAVLLIDGPEGTEVLQGPGTGELLVHTPFQTGGYLEQARNA
                     HAFMPDPRGGGAMFYRTGDLVHRDADGRYFLQGRTDFQVKVRGVRTNTLEVETVLGAHP
                     DVVEACVVALPCPEAGNRLHAEVVRRDGASLSSLGLRAYAAKHLPRHAVPSSVGLAGTP
                     LPKTPTGKPDRNSIKNTRLNGDAP"
     CDS_motif       complement(10975..11034)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B083_RS0105915_0002"
                     /evalue="5.80E-06"
                     /label="NRPS-A_a8"
                     /locus_tag="B083_RS0105915"
                     /protein_end="392"
                     /protein_start="372"
                     /score="18.9"
                     /tool="antismash"
                     /translation="GRTDFQVKVRGVRTNTLEVE"
     aSDomain        complement(11005..12147)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B083_RS0105915_AMP-binding.1"
                     /evalue="2.00E-58"
                     /label="B083_RS0105915_AMP-binding.1"
                     /locus_tag="B083_RS0105915"
                     /protein_end="382"
                     /protein_start="1"
                     /score="189.9"
                     /specificity="substrate consensus: Ala"
                     /tool="antismash"
                     /translation="QEQPDSTALVASGERVTYAELERLTRLAAADLDGAGFGFVRALCV
                     PAHKSPETIALLLAAWRQGINTLVPSPSLGAAALATLTAQAHGPLTATAVPGGGVVLAR
                     EETDPALADGFVVPDPAESLLTLTTSGSTGVPKLVPIRTEAFDRFADWATGKFGLDGST
                     RALSFSPLNFDLALLDVWTVLEAGGTVVLADAGASGDAGYLRELALAHEVTLVQGVPLL
                     HRLLAADGARYPAVRTVVFTGEAVSEQGLRDAFAACPAARFHNVFGCTETNDSFIEDVD
                     PDAYVHPLPIGTPIDGTDAVLLIDGPEGTEVLQGPGTGELLVHTPFQTGGYLEQARNAH
                     AFMPDPRGGGAMFYRTGDLVHRDADGRYFLQGRTDFQVKVR"
     CDS_motif       complement(11725..11769)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B083_RS0105915_0001"
                     /evalue="4.90E-04"
                     /label="NRPS-A_a3"
                     /locus_tag="B083_RS0105915"
                     /protein_end="142"
                     /protein_start="127"
                     /score="13.1"
                     /tool="antismash"
                     /translation="LTTSGSTGVPKLVPI"
     misc_feature    12353..12366
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.22"
                     /tool="antismash"
     misc_feature    12384..12399
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            complement(12492..12935)
                     /locus_tag="B083_RS0105920"
     CDS             complement(12492..12935)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489542.1"
                     /locus_tag="B083_RS0105920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_018489542.1"
                     /transl_table=11
                     /translation="MTINQQPRAASDQTMWEAVLGLHAFVERQLAHTLQRRYGVGLSEY
                     RALETLHQAENGECRMQELADHIGLGQSSVTRLVGRLDSAGYAYKDSCTDDKRGVFAVI
                     TEEGRKRYQEARATYADVLSSALNTASADAQLARAVQALRSAA"
     misc_feature    complement(12973..12986)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.74"
                     /tool="antismash"
     misc_feature    13029..13042
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.24"
                     /tool="antismash"
     misc_feature    complement(13029..13044)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.67"
                     /tool="antismash"
     gene            complement(13066..14169)
                     /locus_tag="B083_RS0105925"
     CDS             complement(13066..14169)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452971.1"
                     /locus_tag="B083_RS0105925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_232790294.1"
                     /transl_table=11
                     /translation="MHETPEMATGRALDGLSRLLFGHLHRSDQQRWGHVYLQGLLRTHG
                     KKTVRRMAATVTGSEAASSSLHQFVNASPWEWTPVRGRLARWTARTLPVRSWTLAPTVL
                     PKRGDHSPGVHQRFLPESGRTVNCQLGLGLFLDAGQAHIPVDWQLFLPPRFTRDPAVRD
                     RAKIPATAQARPLWDQALHLVQEMTSWTDDGRTAPVVADLTALCDIAPLAQRLQGSGHA
                     FVLAIPGQTALGGPELPPGERPSARALLLRRGVRHPAGAGRQIISYETPVRGVRPSGEH
                     TQGAGRLMAEWDAGTGRPGRIWLTNITDRHPAELLELARSPRGAQSAVRRMGEDFGLLD
                     FEGRSFPGWHRHMTLVSAAYACATLEA"
     misc_feature    complement(14059..14061)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            14291..15226
                     /locus_tag="B083_RS1000000136665"
     CDS             14291..15226
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="B083_RS1000000136665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_232790295.1"
                     /transl_table=11
                     /translation="MQERAARTRQTVLEAAADEFAERGYEGASLQRVAWRAATSIGALT
                     FHFRNKTALADEVRTTGRDRFGRCLAELAPAADPLRELRTLIATLARLAHEDRFVRAAR
                     RLEADPPEGAPPLAETWLPVLRDLLDRAHGAHRLRPGVAPEDAVAMLAHLAEGAMAAHG
                     TPRDAAWDALWDSVWDVVLHGLAADRGEGGGGDERDGRGDRTDGDDPGEPGEHGGRGDR
                     TDGDDQGVRGVRGDRGDRRDAADRGNRGGRVARGDGEDRGAGTGRDDRGDRDGQGGRGE
                     RGERGGRRDRVAASGDASPDMTAAARRGGG"
     misc_feature    14528..14530
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 gctatctgct cgaccagttc ctcaccccgt acaccaatct gcgcaccgac gggtacggcg
       61 gggacgccga ggggcgctca cggctcgtgc gggaggtgac ggccgaggtc agaggcgcgg
      121 ccgggcccgg cttcacggtc ggcgtccggt tctcccaggg gaagatcaac gacttcgggt
      181 ggcggtggcc gggcggcgac gccgaggcgc gcgtcctctt cacggcggcc gccggggccg
      241 gtgccgacta tctgcacctc gcgggcagcg ggcgggactg ggcgccggac gggccgacgc
      301 tccccgggct cgcccggcag gtcaccgggc tccccgtcat cgccaacggc gctctgcacg
      361 aggacgcggc ggcgcggcgc gtcctggacg agggacacgc cgacgtgctg gccgtcggga
      421 cgggcgcact ggccaacccc gacctgccgc acaaggtggc cgcgggggtt cctccggtgc
      481 cgttcgatcc gcgggtgctg gaaccgctgg cgaccttgga caacgcccgt acgcacgcaa
      541 ccgcttgaca ccagttacat gcacatgcaa gattaagtcc atcgggtcgc gtcaacggcg
      601 gagacttgtg gaaccatccg attgcatgtg caggcaaatg aacgttacag ggcaactcgg
      661 gagacgaact gccatgggta cacacgacag cgaactgatg ggcttcgccc acgaagcgat
      721 ccggctcagc cgcgagcacg tcgcccaggg cggcatcccc ttctccggtg tcgtcgtggg
      781 cggcggacgg atcctgggga ccggcttcaa ccgggtccgc gaggacaacg acccgaccgc
      841 gcacgccgag gtcgtcgccc tccgcgaggc caccacccgg cacggcatcc gcgccgtggc
      901 gggggccacc ctgatcgcct cgggcgagcc ctgcgccctg tgctacatgg cggcgcggtt
      961 cgccggtatc ggccacatcg tgcacaccgc cgaccgccgg accgccgccc ggtacggctt
     1021 cgactacacc agcacctact ccctcttcgc cgaggacccc gagcgctggc ccatcaaggt
     1081 caccgcgctc caactccccg aggccgaggc cccgttccag gacttcctca ccatgagccg
     1141 gacccggcac tgaccacggc cccgacccgg gccccaggcc ccgggcccgc gacatcgccg
     1201 tacaccgccg cacgcaccgc ctcccccacg cccactcccc tcctcaccca ccgcacggaa
     1261 ggacctccca tgcactggct ctacctcgcc atcgccgtcg ccttcgagat cgctgtcgcc
     1321 atcgccgccg gaaaggcgga gggcttcaag aaccgcaagt ggaccggcat caccctggcc
     1381 agcggcgccg ccgcgacctt cttcctcagc aaggcgctgc tgaccttcga cgtcggcgtc
     1441 ggctacgccc tgtggacctc cgtcgcgggc gtcggtatca ccatcctcgg cgcgctcttc
     1501 ttcggtcagc gcctgaactg gaacaaggcg atcggcatcg tcctcgtcat cggtggcgtc
     1561 gtcggcctcc agctgagcgg ttccgcctga gcccgggtgc accacccccg gccgcgcacg
     1621 cggcgccccc cgcacaccat ctgacgccat ttcagagagg atccacctcc catgtccaac
     1681 gcggtcaaga accccaccaa cgcctggctc tcgctcctgc tggccggagt cttcgagatc
     1741 ggatacgcgc tcgccgtcgg cggcagcgaa ggcttcaccg tcctgacctg gtccctggtc
     1801 gccgtggtgt tcttcctgct gacgctctac gcactgagcc tggccctgcg cacgatcgag
     1861 gtcagcatcg gctacgcggt ctgggccggc atcggcgcgg tcggcgccgc catcctgggc
     1921 ccggtcttct tcgacgagac cctcaccctc gccaagggca tctggctgac ggtcatcatc
     1981 gtcggtgtca tctggctgaa gctggccgac cgcacgaagc ccgaggcggt cgccccggcc
     2041 ccggcggccc agcaggtcac ggaccggccc ggcgcgctcc aggtctgacg cgccacgacc
     2101 gcagccacga aggccgcagc cacgaaggcc gtagccaccg ccaccgtacg cgccacgacc
     2161 gtacgcccca ggcggctgcc tcccatcagg aatgggcggc agccgccggg ggcgttccgt
     2221 catgtcgcgt accgcagcgg tcacttggcg gtgcgcccgc cgtccaccgc gagggcggtc
     2281 ccggtggtga aggaggaggc gtccgagcag agccaggcgg cggcgcgggc gatctcgacc
     2341 gggtcggcca gccgccgctg gatgccctgg gagccgaagc ccgcgcccac ggcggggccg
     2401 ggcgggcgga gggcgtccat catctcggtg tcggtgccgc ccaccacgag ggcgttgacc
     2461 cggatgccgt gctcgccgta ctcggccgcc gccgcccggg tgaggccgat gaccgcgtgc
     2521 ttggcggcca cataggcggc cgacgcggcg gtggcccgga cgcccgcgac gctggaggtg
     2581 ttgacgaccg cgcccccgcc cgccgccatc agggcgggca gctgggcgcg caggcagttc
     2641 cacaccccgc gcagattgac gtccagggtg cggtcgaagg cgtcgtcggg gagttcgtgc
     2701 aggagcgttc cggcgctggc gtgaccggcg ttgttgaacg cgcagtcgag gcggccgaac
     2761 cgctcgaccg ccgtggccac ggcccgttcc atgtccgcgg ggcgggtgac gtcgcccggt
     2821 acggccacgg cccggccgcc gtccttgcgg atgcgctcgg cgccctcctc cagcagggcg
     2881 gcccggcggg ccacgagcac gacggcggcg ccctcgtcgg cgaagtgccg ggccgcggcg
     2941 gcgccgatcc cgctggaggc gccggtgacc agcgccacct tgccggacag cagcccgccg
     3001 cccgccgccg ctctgccgga cggcggctct ccgcccgccg ccgctctgcc gggctgcggc
     3061 cctccgcccg gcgtcacgcg tacccgcccc ggccggacgc ggacgcgtac ccgcccgggc
     3121 ccgccgcggt gaggggggcg ctccagaagg tgtccgcgtt catctcctgg tcgatccggc
     3181 gcagcaggcc gccgtcgagc gtgtccaggc ggtcgatcag gtgccgtacg cccgcctccc
     3241 gcatgagtgc gcgctggtgg ctgtgggcga aggcggaggg gcggccgacg aaggccgtgc
     3301 ccagctcgcg ggccgcgtcc gcgacccggg cgacgtcgtc gacgaagagg gcctgggcgg
     3361 ggcggaggcc gaagacgtcc tcggtgatct cccggaggcc ggggcggaag tcgttggtgc
     3421 agacatagcc ggggccgtcg aacaggtggg cgtagcggcc cagatggtgg tcgaagtgct
     3481 ccttgcccag gccgccgtag cagactgtgc gcaggcccgc gctccgcagc cggtggagca
     3541 gctggcgggt gcccggcgtg atcatgaccg ggtggcggtc gacgtgggcg gcgcgctcct
     3601 cgaagtaggc ggcgagcgcc gcctcgccgg tcatgccccc gcccacggcg gccgcgagcc
     3661 ggcggccggc tatgtgcctc ggctgggaga agacggatcg ctccagggcg gcggagtacg
     3721 taccgcccct gctggtgacg aactggtgga tgaccggact gaaggtgtcg ttgagcagga
     3781 caccgtcgat gttcagcgcg gccaggcgta aggaaagcat gcggctccgt agggatcgtc
     3841 cggggcgggg gaacggtcgg cgggcagagc ggtcgtgggg cggtcgaggc gtgtgcggtc
     3901 gggacgccct cggccacggg cgcccgtgat caggacgccc gtggccggag tgcggttcgt
     3961 cgggtgcggt cgggggagtc ggggcgatcg gtcggtgcgg gcggccggcc cggtccgtgg
     4021 accggtgcgg tcagggcatc atcgcggcgc cccgggcggg ggtcgcctcg gcgcgggtga
     4081 tcacggacgg caccgcgata cccgggagga tcagattcca catctgctgg gtgcgctcgg
     4141 ggaggtcccg gcgtccgctg acgacgttgg agtacatctg catcccggta caggcggcga
     4201 ccacgaactc cgccaccacg gccggatcga cacccggctg cagctcgccg tcctcctggg
     4261 cctcctccag atagccggtc atgatcgcgc ggaacttctc gtacgggtcg gcgttcgcgg
     4321 ggccctccgt ggaggtctgc tcgttggtca gccgtacgcc ggcccggagc agcggatcgc
     4381 gctggagctg ccacgaccag acgagcgtga ggtcgatgag ccgctgcagc ccgcctgcgg
     4441 cgaccagcgg gaccagggtc tccggctggc tgttcatcac cgccttggcc aggtcctcct
     4501 tggagccgaa gtggaagtag agcgccccgg ccgtcacacc ggcgcgtttg aggatgcggg
     4561 tgatgctggc tcccgcgtag ccgtactcgt cgaagacttc tgccgctgca tggaggagag
     4621 cgcgacgggt ctgttccgct cgctcctgtt tcggttgcgc catgcccttc accttcccga
     4681 aaagaatgct ccttatgtta cctggcccga tcgccgccaa gttgagtgcc ttttcgccaa
     4741 gttgagctgg atatgcaccc tgcttgcctt ggaggaaacc tggccgccca accccttgac
     4801 ccagtaaaac aaaagaaaca ttatttttgg gtcggtgcat ggagtgccct gagcgcgcct
     4861 ctgtgtagtc gtccacgagc aatcaacgat ctggggggtc cacgttgtct gtttccgtgc
     4921 tgagacgcga cgcgcagccg gtccggcagg cgcctgtccg gcaggcgccg gtcgagcggc
     4981 cggcggtccg gcggtcgccg gtgcaccagt ggcaggtgca caagcttcgg ggtgaggaag
     5041 tactgctcag cacgtgggcc gagccgtgcc cgggacgctt cgtgatcacg gcgcgctggc
     5101 cgaaggggca cgcgttctac cggccgtcgc agggcgccta cgacccgctg ctgctcgcgg
     5161 agaccgtacg ccaggcggtt ccgctgctct cccacgccgt gcacggcgtc ccccgggagc
     5221 acaggcaggc ctgggagtac gtgagcttcg cggtcgagcc gctggcctcg ctggtcacca
     5281 cggcagagga cgaggtccgc ctggtcgtca cctgctcgaa cgtggtccgg cggggcaccc
     5341 ggttcgccgg gatgaccatg gacatcgatg tccacctggg gaaacggctg atgggcatcg
     5401 cgcacacccg cttcaacaac cagcccgagg ccatctaccg cagactgcgc gggagttacg
     5461 cggacatcca caccgccctg gagcactgcc tgccgacggg cccgccggtg gagccgcagc
     5521 gggtgggccg gtgctccccg gccgacgtgg tcctctctcc cgcgctgggg ggcgagagac
     5581 ccggccggtg gctgctgcgc gtcgaccccg cccacccggt cctcttcgac cacccggtcg
     5641 accatgtgcc cgggatgctc ctgctcgaag ccgcccggca ggcggcgcac gccgtcgacg
     5701 gcaccgccgg cgcggccgtc atcgggatgc gtacggactt cgtccggtac gtcgaactcg
     5761 acgcccccgc cgtcgtcgtg gccggatccc cggtccccgc cggtacgggc cgccgccgcg
     5821 tccccgtctc cgtcgagcag cacggcgtcc gcgtcttcac ctccgaggtg accctcgcct
     5881 gaccggctcc accggcccca ccggctccat gacgtgacgc ggcgggcggc ccgtacctcc
     5941 cctccacaga ggtacggacc gcccgccact ggtcagacct gctccaactc cggcatcgga
     6001 taagggagtt cgtcatgcat gacgacttcc aggtgcaccg aggccgggac cgcgaagctg
     6061 gcctcgtacc cgggtggggc gatcagctgc cgcacccgcc agccgagcgg ctggccggtg
     6121 aggtccagcc ggttcatgac ggccgccatg ccctggcccg tcgccttggc catggcctcc
     6181 ttgcgggtcc acagccgggc gaagtcccgg accagttcgt cgtcggggcg gccgcccagc
     6241 cactgctgct ccctcgggtg gaggaagggc agatagccgg cgaccgactc cggggtcgga
     6301 tgggactcga tgtcgatgcc gatggtggcc tcggcgaccg cgatggcgac gtggtccccg
     6361 ctgtgcgaca tcgagaactc cggtggctcg ccgccccggt ccggatcgcg gatagccggt
     6421 ctgccgtgca ccccgccgca gccggggcac ggctgccgca ggaatccgac gctccggggc
     6481 gggcagccga gcgaggaccc gaggacgctc cgcagcgccc agtgggcgaa ggcgtaggcc
     6541 tccttgccgg agtgctgcgg gatcgcggcc agccggctcc gctccttctc gtcgagccag
     6601 tcgggcgggg acccgtcggg cggcaggcgc tgcccggccg gggcccggac gatccagacg
     6661 gccacctggt cgcggatctc gcccggaggc gcgtcggacc acttgaggtc gtgtgtcatg
     6721 tcgatacggc tcctgatgtg gcggggaccc ggccggtccc cgccacgggt cggatcaggc
     6781 ggttgctgcg agcagcggcc acgggccggt cgtggcgtac tgctccagac cctcggcggc
     6841 gagatagcgc cggaaccccg gcacctgctc cgccgggcgc agcgccccgc ggtacagcac
     6901 caggccgcgc acgccgttgg cgtcgtcgtg ccagcacacc aggggcatgt ggtactccgg
     6961 cgtgtcacag gtctcctgca cggcggcctg gtagccgtac gcgggcagca ggctcatccg
     7021 gtcacgctcc cgggttccgg gcggacgccg aacagggcgc cgtagtcgct gatccgcgcg
     7081 gccgccccgg tgccgccggc caggtgcagc gcgtgccaga agagcacctg gttggcggtg
     7141 aggacgggga ggccgatccg ctcctccagg cccgcgatgg tgcccacggc gcggatgccg
     7201 ttgccggcga ccaggaccgc ctgcggccgc tgcggggcga cggtggactc gatccactgc
     7261 accagggcct ccggggtgat gagcttctgg ttgctgggga gcccgcaggg ggcgtggtgg
     7321 acgacgtcga acccccgctc accgaagtac gaggcgccca gggccgacag ctcgtcgtcg
     7381 aaccacggcg ggttgaccag cgcgatccgc tcggcgccca gcgcgcggaa cccggccgcg
     7441 gccgcgtggg tggtgccggt gagcgggata ccgcgggtcc gctccgcgag ccgctcgaag
     7501 agcttctgct cgccgtcggc gcccaggacg taggcggagc tggtgaaccc cagggcgatg
     7561 gagtccagcg gggaggccgc gagcagttcg acggcgtcgt cgatgaaggg cgggtcgatg
     7621 aaggcgcgca ccgggtcgtg cgggatcttg gggtccatct cgccgccggg ccgcatcgcc
     7681 ccgaagtgga cgcgggagcc gtggatgtag acgtcgcgcg gggccatcgc ctggagctcg
     7741 gactccggcc cgacgtccgc gtgcggaacg acgacaccga cgcgggcgac ggcgccccag
     7801 ccgtcctctc gggcctggaa ctgcgtcatg gggtgcttct cctcttcttc tgccgtgctt
     7861 ctgctctgac gtgacctgac gtgacgtgac gtgcctgcgg gggaggggga ggggctcagc
     7921 tcgcctcgtc ggccagcgag ccgccgctga ccgttccggt gccctccgcg tcgaggaact
     7981 tcgggtgctt gcggcgcatg ttgcccatga cgaactcgta ggggacgtcg tcgaagtcgc
     8041 cgtggtcggt gaggaactcc atgctctgcc gcccctgcgc gtcgaagggg tggtagaggc
     8101 tgtcggagcg gccgtcgaac tccagcgggg tcatgccgta cagccggcac agcagcttgt
     8161 tgaagaccgg ggtcacctgc agccaccggc cttccaggta cacggtggcc aggccgtgga
     8221 agaagacgtc gccgccgatg taggagcgca gccggtcgga ggcgaggtgg ttgcggacgt
     8281 cgctgtagtg cagccgggcc gggatgccga cggagcggca ggcggcggcg tagaggacgg
     8341 acttgtgcag gcagaagccc ttgccgccgg tgatgatgct ggacgccttc agcccctgcg
     8401 gcgacaggtc ggcgccgtac acctcgtagt ggattccgtc gcgcaccgcg tagtagaggg
     8461 cgacggcgag ttcgacgggg gtggcggtgt cgcggtcgac cccctggagg gccttgtcca
     8521 cgaaggcgcg tacggtctcg tggtcgtggt cgaggaagtc ggtggccgcg gtgaggcggt
     8581 cgaggtcctc ggcggccggg ggcgcgggga cggcgtcggt cagggtgtcg gtcatggcgg
     8641 atgcctctcg tcacaggccc atcagggcgg cgatgcagtt gcgctggatc tcgttggtgc
     8701 cggagtagat ggagccggag gtggcgttgc gcagctcgcg ctcgatgccg gtgtcggtca
     8761 gatagccgcg ggcgccgaag atctggatgg cgtgctgggc ggtgcgctgg ttggcctcgc
     8821 tgaccacgat cttggtggcg gccacgtcca gcgaggcgtc cgtgccctgc tcgaccttgc
     8881 gcccggtgtc gtacagccac ttgcgggccg tctccaccgc gatcctcatg tccgcgatct
     8941 tgtgggagac cgcctggttc ttgccgatgg ccgtgccgaa ctgctgccgg gtggtggcgt
     9001 gttcgacgac gcgcttgagg cggtgggtca tctcgccgag gttgacggcg aaggagaaga
     9061 ggacctcccg cttcatcacg tagtccatga tcaggaagcc ggcgccctcg ctgccgagcc
     9121 ggttggcgac gggcagccgg acgttgtcga gcgtgacctc gctgatcggg gaggtgcgca
     9181 gccccatcgt ctccagcggc tcgccgatcc tcaggcccgg cgtgttgcgc tcggcgagga
     9241 aggtgctgag cccgaaggcg cccggcttgc cggtgcggac gtagagcagg aagaggtcgg
     9301 cgacgggggc gttggtgatg aacatcttgc cgccgtcgat cacgtactcg tcgccctcgc
     9361 ggacggccgt ggtccggatg ttcatgacgt ccgagccgtg gcccggctcg gtgatcgcgt
     9421 gcgcggtgat ggcctcgccc gaggtcaccc gcggcagatg gcgcgagcgc agctcctcgg
     9481 agccgaacgc ctggagcggg atgccgacgc tgacgatctg cgtggacgcc gagaagttga
     9541 ggcccgcgtc ccggctggtg tggccgaggc cctccagcgc ggccatggtc tcggtgagcg
     9601 tccggccggc gccgccgtac tccggggcga aggggagggt gaagaggccg gagcgctgga
     9661 gcggcttcca cttctcctgg gggaactcgc cggcccggtc ccagcgggcg acgtcgtcgt
     9721 tcagctcgtc gccgtacggc gctgtgatgc cgtaggtgtc tgtgtccttc tctgctgcca
     9781 tgagggctct gccttgcctt tcgccggttc gccggtcgcg ggatgggccg ggtgcggggc
     9841 cgcgccgtcg cggccccgca cccggcggcg ggatcagtag cgggagaagc cgtcctcgga
     9901 gagcgagtgc ttctcgtcgc cccgcagcgc cgggctgaag acggagacca ggaggaggtc
     9961 ctcgtcgtcg gaggcgatca ggtggtgggc gtcgtgctgg tccagggcgt agatggtccc
    10021 ggtcttgatc ttgtggcggg tgccgtcggt ggtgacgacc tcgcccgagc cgcctatgca
    10081 gtagcaggcc tccaggtggc cgaggtactg cagcccgctc gtggtgcccc ggcggaccag
    10141 cgtctcgcag acggtgaagc ccataccgtc cgcctcggtg agcaggcggt ggctggtgcc
    10201 gctgccccac tcgaccgcgg ggacgtcgga gagagaacgg acgatcatgg gagtactcct
    10261 gtcatcgctg ttgtctggac gtgttctggg cctgtgctgg tgcgcctggt gcgcctggtg
    10321 cgtgtgtgcc ggtgcgttcg tgcctctgtg cgtgcgcgtt ccgtgcgtgc gcgtcctgcg
    10381 cgtgcgcgtt ctgtgcgcgt ggggggaggg gtggtacggg gatggggcgg tggggctcgt
    10441 ggggtcagac ggaggccggg gcggcctgcg cgacgaagcc gcggatgtcc gcgagcgtgc
    10501 ggtaggcggc cggctccagg gggatgtcgt cgacggggat gtggtacgtc tccgatatcc
    10561 aggagatgag gcggaccagg ccgaggctgt tgacgacggt gctctcgatg aggtcgaagt
    10621 cgtcggtcag gtcctcggcg tcctcgccgg cgaggaactc gttgacgatg taggcgcgga
    10681 tcgggtcgtg ggcaccggtg ctcatggggc gtctccgttc agtcgggtgt tcttgatcga
    10741 gttgcggtcg ggcttgccgg tcggggtctt cggcagcggg gtgccggcga gcccgaccga
    10801 gctgggcacg gcgtggcgcg gcaggtgctt cgccgcgtac gcccgcagtc ccagcgagct
    10861 gagggaggcc ccgtcccggc gcaccacttc ggcgtgcagc cggttgccgg cctcggggca
    10921 gggcagggcg acgacacagg cctcgacgac gtccgggtgg gcgccgagga cggtctcgac
    10981 ctccagcgtg ttggtgcgca cgccgcgcac cttgacctgg aagtcggtgc gcccctggag
    11041 gaagtagcgg ccgtcggcgt cgcggtggac gaggtcgccg gtgcggtaga acatggcgcc
    11101 gccgccgcgc gggtcgggca tgaacgcgtg ggcgttgcgg gcctgttcga ggtagccgcc
    11161 ggtctggaag ggggtgtgca ccagcagttc accggtgccg ggaccctgga ggacctcggt
    11221 gccctcgggc ccgtcgatca gcagcaccgc gtcggtgccg tcgatcgggg tgccgatggg
    11281 cagcggatgg acgtacgcgt ccgggtcgac gtcctcgatg aagctgtcgt tggtctcggt
    11341 gcagccgaag acgttgtgga agcgggcggc cgggcaggcg gcgaacgcgt cccgcagccc
    11401 ctgctcggag acggcctccc cggtgaagac gaccgtgcgg accgccgggt agcgggcccc
    11461 gtccgcggcc agcagccggt ggaggagggg cacgccctgc acgagggtca cctcgtgggc
    11521 gagggccagc tcccgcagat aaccggcgtc cccgctggcc ccggcgtcgg ccagcacgac
    11581 cgtgccgccg gcctccagga ccgtccagac gtccaggagc gccagatcga agttgagcgg
    11641 cgagaagctc agggcccggg tggagccgtc gagcccgaac ttcccggtgg cccagtcggc
    11701 gaaccggtcg aaggcctcgg tgcggatcgg gacgagcttg gggacgccgg tggacccgga
    11761 ggtggtgagc gtcagcagcg actcggcggg gtcggggacg acgaagccgt ccgccagggc
    11821 agggtctgtc tcctcccgcg ccaggaccac gccgccgccg gggaccgcgg tggccgtcag
    11881 cgggccgtgg gcctgggcgg tgagcgtggc cagggcggcg gcgcccagac tcggggaggg
    11941 gaccagggtg ttgatcccct ggcgccaggc cgccagcagc agggcgatgg tctccggcga
    12001 cttgtgggcc ggtacgcaca gggcgcggac gaatccgaag ccggcgccgt ccaggtccgc
    12061 ggcggcgagg cgggtcagcc gctccagctc cgcgtaggtg acccgttcgc cggaggcgac
    12121 gagcgcggtg ctgtcgggct gttcctgcac atgtctgctg aatgcctgta acaggcgagg
    12181 cgatggcaac ggtccccctc ggtggttggg cgatctggcg gcaggatccg gctgtgcggt
    12241 ccggctgcgc gatcgagctg tgcgagccgg gtacgcgatc gggcctgagc cggatgagcc
    12301 ggccctgagg ccgcgagatc tggctacgag agagaatcta cgggtctcta tttgcatggt
    12361 caagcaaata gatagagcgt tcgtgtgatc caggccacac cctcgccggt tgtggaagaa
    12421 gcccgaacat gcaaaagacg gcgggggtgc cgggtcaccc cggccgcctc ccgccgtctg
    12481 gcgtgatcgt gtcaggcggc gctgcgcagc gcctgcaccg cccgggcgag ctgggcgtcg
    12541 gcgctcgcgg tgttgagggc cgagctgagg acgtcggcgt acgtggcgcg ggcttcctgg
    12601 tagcgcttgc ggccttcttc ggtgatgacg gcgaagaccc cgcgcttgtc gtcggtgcag
    12661 ctgtccttgt aggcgtatcc cgcgctgtcg agccgtccca ccaggcgcgt caccgaactc
    12721 tggccgagcc cgatgtggtc cgccagctcc tgcatgcggc actcgccgtt ctcggcctgg
    12781 tggagggtct ccagggcgcg gtactcggac aggccgacgc cgtagcggcg ctggagggtg
    12841 tgcgcgagct ggcgttccac gaaggcgtgc aggccgagga cggcctccca catggtctga
    12901 tcggaggcgg cgcgtggctg ctggttgatc gtcatggaac agctcccctt ctcgactgcg
    12961 ataacaacaa gatacatgcc catgcaatca tctggggagt gccggagccc acggggtggg
    13021 ctctggccga tatttatttg tatgcgcaag taaagcgtca ggaggtcatg cctccagggt
    13081 ggcgcacgcg taggcggccg agacgagcgt catgtgccgg tgccagccgg ggaaggagcg
    13141 gccttcgaag tccaggagcc cgaagtcctc gcccatgcgc cggacggcgg actgcgcccc
    13201 ccggggcgag cgggccagct ccagcagttc ggccggatgc cggtcggtga tgttcgtcag
    13261 ccagatccgg cccggacgcc cggtgcccgc gtcccactcg gccatcagcc ggcccgcccc
    13321 ctgggtgtgc tcaccgctcg ggcgcacccc gcgcacgggg gtctcgtacg agatgatctg
    13381 ccggcccgcg cccgccgggt gccgcacccc gcggcgcagc agcagtgcgc gcgccgacgg
    13441 cctttcgccc ggtggcagtt cgggtccgcc gagggcggtc tgccccggga tggcgagcac
    13501 gaaggcgtgc ccgctgccct ggagccgctg tgccagcggt gcgatgtcgc acagggctgt
    13561 caggtcggcc accacgggtg cggtccgccc gtcgtccgtc caggacgtca tctcctggac
    13621 caggtgcagg gcttggtccc acagcggccg ggcctgggcc gtcgcgggga tcttcgcccg
    13681 gtcgcgtacg gcggggtccc gggtgaaccg gggaggcagg aagagctgcc agtcgaccgg
    13741 gatgtgcgcc tgccccgcgt ccaggaacag gcccagtccc agctggcagt tgacggtacg
    13801 cccggactcc ggcaggaaac gctgatgcac tccgggcgag tggtcgcccc gcttgggcag
    13861 cacggtcggc gccagggtcc agctccgcac cggcagtgtc cgggcggtcc agcgggccag
    13921 ccggccgcgg accggggtcc actcccaggg gctggcgttc acgaactggt gcagcgacga
    13981 cgacgccgcc tcggatccgg tcacggtggc cgccatgcgg cgcacggtct tcttgccgtg
    14041 cgtgcgcagc agcccctgta agtacacatg gccccaacgc tgctggtcgg agcggtgcaa
    14101 atggccgaag aggaggcggg acaggccgtc cagcgctcgg cccgtcgcca tctcgggcgt
    14161 ttcgtgcatc gtcgtcactg caggtccctc ccaggtgagc gtcgctgagc agactaacaa
    14221 acatagagaa tgttcgttat atttctgagg gagaagcgcg acgacttacc gaggttcaag
    14281 gagaaggtgc atgcaggaac gggcggccag gacgcgccag accgtgctgg aggcggcggc
    14341 cgacgagttc gccgagcgcg gttacgaggg cgcctccctc cagcgggtgg cctggcgggc
    14401 cgcgacctcg atcggtgctc tgaccttcca cttccgcaac aagaccgccc tggccgacga
    14461 ggtgcgcacc accgggcgcg accgcttcgg ccgctgcctg gcggaactcg cccccgccgc
    14521 cgatccgtta cgggagctgc gcaccctgat cgccacgctg gcccggctgg cgcacgagga
    14581 ccgcttcgtc cgcgccgcac gccgtctgga ggccgatccg ccggagggcg caccgccgtt
    14641 ggccgagacc tggctccccg tcctccgcga tctgctcgac cgggcccacg gcgcccaccg
    14701 gctccgcccc ggcgtggcgc ccgaggacgc ggtggcgatg ctggcccacc tggcggaggg
    14761 cgcgatggcc gcccacggca ccccccgcga cgcggcctgg gacgcgctct gggactccgt
    14821 ctgggacgtg gtcctgcacg gcctcgccgc ggaccggggg gagggaggcg gcggggacga
    14881 gcgggacggc cggggcgacc gcaccgatgg tgacgacccg ggcgagccgg gcgagcacgg
    14941 tggccggggc gatcggaccg atggtgacga ccagggggtc cggggagtgc ggggcgaccg
    15001 aggcgatcgg cgcgacgcgg ccgaccgggg gaaccggggc ggtcgcgtgg cgcggggcga
    15061 cggggaggac cggggcgccg ggaccggccg ggacgaccgg ggcgaccggg acgggcaggg
    15121 tggccggggt gagcggggtg agcggggcgg tcggcgtgac cgggtggcgg cctcggggga
    15181 cgcgtcgccg gacatgaccg cagccgcgag gcgcggcggg ggctgagccc gtcgccgtgc
    15241 ggggcggt
//
