LOCUS       NZ_KI912410            10834 bp    DNA     linear   CON 02-MAY-2024
DEFINITION  Actinomadura oligospora ATCC 43269 P696DRAFT_scaffold00001.1, whole
            genome shotgun sequence.
ACCESSION   NZ_KI912410
VERSION     NZ_KI912410.1
KEYWORDS    WGS; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Actinomadura oligospora ATCC 43269
  ORGANISM  Actinomadura oligospora ATCC 43269
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 1148980)
  AUTHORS   Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K.,
            Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A.,
            Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2014) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            environment                       :: Soil
            investigation_type                :: bacteria_archaea
            project_name :: Actinomadura oligospora ATCC 43269
            sequencing_meth                   :: WGS
            ref_biomaterial                   :: ATCC 43269
            GOLD Stamp ID                     :: Gi22378
            Type Strain                       :: Yes
            Isolation Site                    :: soil, India
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Unknown program v. before 2013-09-10
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000518265.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 02:24:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,332
            CDSs (total)                      :: 8,253
            Genes (coding)                    :: 8,185
            CDSs (with protein)               :: 8,185
            Genes (RNA)                       :: 79
            rRNAs                             :: 7, 2 (5S, 16S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 7 of 68
            Pseudo Genes (incomplete)         :: 65 of 68
            Pseudo Genes (internal stop)      :: 1 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:28:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1057122
            Orig. end                         :: 1067956
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KI912410.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 1013217
            Source DNA and organism available from Tim Lilburn at the American
            Type Culture Collection (ATCC) (TLilburn@atcc.org)
            Contacts: Nikos   Kyrpides (nckyrpides@lbl.gov)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI
            Annotation by JGI
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10834
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10834
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10834
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5834](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5834)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(384..1877)
                     /locus_tag="P696_RS0136625"
     CDS             complement(384..1877)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="P696_RS0136625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_026416206.1"
                     /transl_table=11
                     /translation="MTERLAEHPPATADYPSDPADLFVRLYDELPVRLHRAWPLNLWYE
                     DARVRRTAGDLRERITDDGELDPAETAAIVAADEDRGRFTLLLALDGALALANPYGPFH
                     DASALTGITVRYLVEGRLDSGAAGGALLPRCALPGRPDGLRTLPDFFGVHRVPPHLWDG
                     IEHTQLAAVHDPHLSRDVPVEVGCAPLMESYDDVRIGFAERAERTVYRLDPADSAAVRS
                     RVRTIIRKLDGAGARIAVLPEATLSDGLLEVWKEAAFDTARPGSPLRLLMLGSGPLGGG
                     DPPPNRAVLIDRWTGTELLVQDKLSPFVLGEQQLRDWAPPGAPRAGTAEEDIAPGATVR
                     LLDCSLGRLAVLICEDLSRADRWDRELMAAGASHLLVPIFSKPILRFRWEEQNAQRQVN
                     TLGAWVVVANSLVVGNTMPHDPAFGERYTCLVAGPAGAARLDYASVLAFGAAGRGDEPG
                     LTADGALPVLHAGAAHEAWLPHWSDDLTEDDPLDGCTLE"
     gene            2057..2761
                     /locus_tag="P696_RS0136630"
     CDS             2057..2761
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="P696_RS0136630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_026416207.1"
                     /transl_table=11
                     /translation="MATTPATTSTTTSTTNPATPPGPGASRGRLAEAERLLETLEHAKS
                     IEEGQFSALGMLITDASLSSDEPALTALKDGLQWLHRTRYLDVPALDPDQREQRGRVLG
                     LIDVAHWALRRMPSGLQLGLRPGGHAARFLVAVAARPGRSNRELAAELGTDDTEISRVG
                     RRLLAAGVVWRRKEWRSNLWDITPRGRAYLSEIGLLPAPADGDAPSPSPAPSGPSRRGV
                     DPDPAPPPRRHP"
     gene            2915..4024
                     /locus_tag="P696_RS0136635"
     CDS             2915..4024
                     /EC_number="4.4.1.1"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1168: O-succinylhomoserine sulfhydrylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF005758.0"
                     /locus_tag="P696_RS0136635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cystathionine gamma-lyase"
                     /protein_id="WP_026416208.1"
                     /transl_table=11
                     /translation="MADGDSTRSVRAGRPAPEQYTPELPGPTFAAHFHLSGEAAGPYTY
                     GRDANPTWTLLEDAISELEGGGATTVFSSGMAAVSAAMLSLVASGDTVVMPDDCYQTTR
                     ALRDRLESYGVRVRMAPTAGDAQLALVEGARLVWLETPSNPGLDVCDIARVADAAHAAG
                     ALVAVDNTLATPFGQRVLDLGADLAVASDTKSLTGHGDLLLGHVTTRDPRLAASIRTWR
                     KTIGAIPGPMEAWLAHRSLATLALRLDRQTANALALATALRDHPLVGGLRYPGLKDDPA
                     HALASRQMRRFGCVVSFTLPSRDAAEGFLGALRLVGEATSFGSVHSTAERRARWGGDAV
                     PPGFIRFSCGIEDAGDLVADVLQALDAVR"
     gene            complement(4021..4455)
                     /locus_tag="P696_RS0136640"
     CDS             complement(4021..4455)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540434.1"
                     /locus_tag="P696_RS0136640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_026416209.1"
                     /transl_table=11
                     /translation="MTETFPDARADRPIGYWLRHIDGALDANLGRLLADDGLTRRGWQV
                     LNTVSYEPITMADLDRTLDAFVSPDEPTMRPQVERLVDLGWARLDGDTAELTEDGVRAH
                     RRVRDTIGAQRARMIECFTPEEFRSLMEMLRRFAAHLETV"
     misc_feature    complement(4471..4487)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.9"
                     /tool="antismash"
     misc_feature    4471..4487
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.86"
                     /tool="antismash"
     misc_feature    complement(4472..4485)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.39"
                     /tool="antismash"
     misc_feature    4475..4488
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.74"
                     /tool="antismash"
     misc_feature    4476..4489
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.44"
                     /tool="antismash"
     misc_feature    complement(4479..4492)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.1"
                     /tool="antismash"
     misc_feature    4480..4493
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.7"
                     /tool="antismash"
     misc_feature    complement(4500..4516)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.72"
                     /tool="antismash"
     gene            4504..5004
                     /locus_tag="P696_RS50545"
     CDS             4504..5004
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895538.1"
                     /locus_tag="P696_RS50545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_051468594.1"
                     /transl_table=11
                     /translation="MSSATGDEDGSAADASDAVAAVERAMVAIRRSQTRRTLGRLAGPG
                     GMDPTLFGVLDAIEGHGGPCAVTDVAAALGVDQPRASRLALRAEEEGLVTRGPDPSDRR
                     RTLLELTATGREGLDRAHRTRRRLFAQVMADWPDTDRDEFARLITAFVTSFGETVTEGR
                     PAG"
     gene            complement(5001..5834)
                     /locus_tag="P696_RS0136650"
     CDS             complement(5001..5834)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083253.1"
                     /locus_tag="P696_RS0136650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_026416210.1"
                     /sec_met_domain="Linocin_M18 (E-value: 2.8e-84, bitscore:
                     278.2, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPISAAAWADLEDEVRRTFTRGVAGRRLVDVPDAAGP
                     ELAAVTTGYLDAADSPADGVTAALRTSRPVVQLTVPFTVSREAVDAVERGAQDADWQPA
                     KDAATKLAFAEDRAIFHGYAPGRIAGIAAEAVTVSPARPLPVEASHYPDVVSEALATLR
                     LAGVGGPYALVLGSEAYTAATRSSDQGYPVYQHLAKLVDGGIVWAPAIDGGVVMSTRGG
                     DYRLSLGQDLSIGYTGHDASGVNLYLLESFTFLASTAEAAVALTGPSPQSHPTGF"
     gene            complement(5831..6895)
                     /locus_tag="P696_RS0136655"
     CDS             complement(5831..6895)
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017540968.1"
                     /locus_tag="P696_RS0136655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_026416211.1"
                     /transl_table=11
                     /translation="MPPQAQPQRPDPQAVLGPLTDAAVFLVVTIEAGAEPVVHELLGDL
                     SGLVRAVGFRVPEGNLTCVTGIGSDAWDRLFSGNRPAGLHPFTALDGPRHRAPATPGDL
                     LFHVRARTMDLCFELATQIMDRLRGAVTVQDEVHGFRYFEKRDLMGFVDGTENPAGSAA
                     ERAVLVRAEDDPEFADGSYVIVQKYLHDLPAWNALPVEAQELVIGRRKLNDIELDDATK
                     PDDSHVAVNTVLGPDGEQRQILRDNMPFGAPGKGEFGTYFIGYAHTPETTEQMLRNMFL
                     GTEDGHTDRILDFSTAVTGCLFHAPTLDFLDSPPDLPDPDSAAVAPPVPAAVPEPAPGD
                     LFGSLGIGGLKETP"
     gene            complement(7115..7582)
                     /locus_tag="P696_RS0136660"
     CDS             complement(7115..7582)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632629.1"
                     /locus_tag="P696_RS0136660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1801 domain-containing protein"
                     /protein_id="WP_026416212.1"
                     /transl_table=11
                     /translation="MAEKSTAAKNDADAETFSAGERAAMKERAKELKAAGRSASRVEKA
                     AQAERDVLAKIAEFPEPDRAMAERIHAIVREAAPNLEPKLWYGMPSYALDGKVVCFFQN
                     ADKFKARYATLGFNDTAKLDDGTMWPTAYALTELTEAAEARIADLLKRAAG"
     gene            complement(7626..8081)
                     /locus_tag="P696_RS0136665"
     CDS             complement(7626..8081)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891852.1"
                     /locus_tag="P696_RS0136665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_034488451.1"
                     /transl_table=11
                     /translation="MQARMQNPAQVLDGAMKPILDIFKAVHTGGVDEQTLELVHLRVSQ
                     INGCSACVDAGVKGGRKAGVSEEKLATTAAWREAPYFSDEERAALELAEAATRLADRPD
                     SVSDEIWDNAATYYDEKQLAAIVMMISVTNLFNRLNATTRQIAGAWG"
     gene            8281..10746
                     /locus_tag="P696_RS0136670"
     CDS             8281..10746
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048304.1"
                     /locus_tag="P696_RS0136670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_026416214.1"
                     /transl_table=11
                     /translation="MVAEQRTTDPQEELAERLRLIQELSGRGVRALARDTGLSSSSLSR
                     YLGGQTVPPWPAVVELCRLVKRDPRPLRPLWERAGSPLPAPPKTSRQVQPPSPPAGTPR
                     PPRNDLPRDVPDFTGREDGLAAVLAAVEADRLVAIDGMPGVGKTCLAVHAAHRLTTGYP
                     DAQLYLDLHGFTEGREPLDPDMALRTLLAALDVPSERIPQAGGIEPLAACWRAELASRR
                     AVVVLDNAAGADQVRHLLPGAGPSVALITSRNRLLGLDEVPPVSLDVLSPEESAELLAR
                     ASGESGRPEGRLAREPEQAAEVLRLCGHLPLALRLAAARLRHRPGWTVGILVERMAEGP
                     SEFDTAFAMSVRQLDRSQRRFFRLLGLLPGSSFDEYVAAAVADVPLRAARTMLEDLLDA
                     HLVQQPSGGRYRLHDLVREHARRATAEQDTQDDRDRALGRVLDYYVHAAAAAESAMPYL
                     SRSRAPTAGEAPAELPAFGHKHQALFWFGTEYTNLMTVFDTAVATGADVHACELPRFMR
                     AFFARRCGTTHLNVLFERALDAARRLDDPLRLAEAHSDLGFARYNAGRMAEAHAAYDTA
                     APLIAGTGDTWAEAELAMRRAFLLWDEGNVQEPLTLFCRAAALYDSIGSLKGRAHATAN
                     EAWALLQLGYREDAAARARQALDLTGDEPDWPSTLTARITLGVALAPENPDEANDHLTR
                     ALELAREDSHKHNEAWCLNCLGVALRQMGRYDEALASHRRAFALLDELFEEHWKIHFLN
                     GYGETCRLAGLPDEALRLHRQALELAPEVGYRHEEALAHEGIAAVLAETDPNTAAEHRA
                     AGLALLQTS"
     misc_feature    complement(8321..8335)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.35"
                     /tool="antismash"
     misc_feature    complement(10757..10771)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.22"
                     /tool="antismash"
ORIGIN
        1 accggacgcg cgggatgccg ctccgcgagg cctggtgcct gccccggatc gtcgtcgcgt
       61 acctgaaact gggccagccc gacaaggccg cggacgtcgg cgcgcacgcc gccgggctgt
      121 tcgcggacat cggcgacgag cacctcggcg gacgcgtcct gtccctgctc gccgtcgccc
      181 tggacgaact ggaccgtccc gaggaggccg cccgcgcccg cgaggccgcc gagacgccct
      241 ggcgccgctc gggcctgtcc ctggacgtcc ccgaccagcg cctcgtcgag aaccggcccc
      301 cggccgaccc cggcctgccg acccgggccg accctgcccg gccgactcag gcggcacccc
      361 gtcggccggg tccggggtga gcgtcactcc agggtgcagc cgtccagggg gtcgtcctcg
      421 gtgaggtcgt cggaccagtg cgggagccac gcctcgtgcg cggctcccgc gtgcaggacg
      481 ggcagcgcgc cgtccgcggt gaggccgggc tcgtcgccgc gtccggccgc gccgaaggcg
      541 aggaccgagg cgtagtcgag ccgggccgcg ccggccgggc ccgcgaccag gcaggtgtac
      601 cgctcgccga acgccgggtc gtgcggcatg gtgttcccga cgaccaggct gttggcgacc
      661 accacccacg cgccgagcgt gttgacctgg cgctgcgcgt tctgctcctc ccagcggaac
      721 cgcaggatcg gcttggagaa gatcggcacc agcaggtggg acgccccggc cgccatcagc
      781 tcgcggtccc agcggtccgc gcggctgagg tcctcgcaga tcaggacggc gaggcggccg
      841 agcgagcagt ccagcagccg gacggtcgcg ccgggcgcga tgtcctcctc ggcggtgccc
      901 gcgcgcggcg cgccgggcgg ggcccagtcg cgcagctgct gctcgccgag gacgaacggg
      961 ctcagcttgt cctggacgag cagctccgtg cccgtccagc ggtcgatcag cacggcccgg
     1021 ttcggcggcg ggtcgcctcc tccgagcggc cccgacccga gcatgagcag ccgcagcggg
     1081 gagcccggcc gggcggtgtc gaaggcggcc tccttccaca cctcgagcag cccgtccgac
     1141 agcgtcgcct cgggcaggac ggcgatccgc gcgcccgccc cgtccagctt ccggatgatc
     1201 gtccgcaccc gggagcggac cgccgccgag tcggccgggt cgagccggta caccgtgcgc
     1261 tcggcccgct cggcgaaccc gatccgcacg tcgtcgtagg actccatcag cggcgcgcag
     1321 ccgacctcga ccggcacgtc ccgggagagg tgcgggtcgt ggacggcggc cagctgcgtg
     1381 tgctcgatcc cgtcccacag atgcggcggg acgcggtgga cgccgaagaa gtccggcagc
     1441 gtgcgcagcc cgtccggacg tcccggcagg gcacagcggg ggagcagcgc gccacccgcc
     1501 gccccgctgt cgagacggcc ctcgaccagg taccggacgg tgatcccggt cagcgcggac
     1561 gcgtcgtgga acggtccgta gggattggcg agggcgagcg cgccgtccag ggcgagcagc
     1621 agcgtgaacc ggccgcggtc ctcgtccgcc gcgacgatcg cggcggtctc ggccggatcg
     1681 agttcgccgt cgtcggtgat ccgctcgcgc aggtcgcccg cggtgcgccg gacgcgggcg
     1741 tcctcgtacc agaggttcag gggccacgcc cggtgcagcc ggacgggcag ttcgtcgtag
     1801 aggcggacga agaggtccgc cgggtcggac gggtagtccg cggtcgcggg tgggtgctcc
     1861 gcgagccgtt ccgtcatgtg cgccctccgg ggggtgacag cgcgcggcag ggggtgactc
     1921 gatgctatcg ccgtcctggt aagggtttcg tgccgtctgg cgcggatccg atgagtcact
     1981 tgacattcac ttggcatcga cggacagtgg aaggacaccg gtcgaagaga cgccgacgag
     2041 aacgaggacc accgacatgg cgacgacccc cgcgacgact tccacgacga cctccaccac
     2101 gaaccccgcg acgcccccgg gccccggggc gagccgcggg cggctcgccg aggccgagcg
     2161 cctgctggag accctggagc acgcgaagtc gatcgaggag gggcagttca gcgcgctcgg
     2221 catgctgatc accgacgcgt cgctcagttc cgacgagccc gcgctgaccg ccctcaagga
     2281 cggtctccag tggctccacc ggacgcgcta cctcgacgtc ccggcgctcg atcccgacca
     2341 gcgcgagcag cgcgggcggg tgctcgggct catcgacgtc gcgcactggg cgctgcggcg
     2401 gatgccgtcc ggcctccagc tcggcctgcg gcccggcggc cacgcggccc gcttcctggt
     2461 cgccgtggcg gcccgtccgg gccggtcgaa ccgcgagctg gcggccgagc tcggcaccga
     2521 cgacaccgag atcagccggg tcggccgccg cctgctcgcc gcgggcgtcg tgtggcggcg
     2581 caaggagtgg cgcagcaacc tgtgggacat cacgccccga gggcgcgcct acctgtccga
     2641 gatcggcctg ctgcccgccc cggcggacgg cgacgccccg tccccgtcgc ccgcgccgtc
     2701 cggcccgtcc cgccgcggcg tcgaccccga cccggccccc cccccccgcc gccacccctg
     2761 acggccccca ccccgaccgt ccgtttccgg gccctgccac ctgcccggcc gccgcctgcg
     2821 gccgtgccct gcccggggtc cttcgtcacc ccgggcgctg gtgctctgac ctgtgcggtt
     2881 acccttcggg cgaaagatcc cgaaggagga cggcatggcc gacggcgaca gcacgcgcag
     2941 cgtccgggcg gggcggcccg cccccgagca gtacacgccc gagctgcccg gcccgacgtt
     3001 cgccgcgcac ttccacctgt ccggcgaggc cgccgggccc tacacctacg gccgcgacgc
     3061 gaaccccacg tggacgctgc tggaggacgc gatcagcgag ctggagggcg gcggcgcgac
     3121 gaccgtcttc tcctccggga tggccgccgt gtccgccgcg atgctgtcgc tggtcgcgtc
     3181 cggcgacacc gtcgtcatgc cggacgactg ctaccagacg acccgcgccc tgcgggaccg
     3241 cctggagtcc tacggggtgc gcgtccgcat ggccccgacc gcgggcgacg cgcagctcgc
     3301 gctcgtcgag ggcgcgcgcc tggtctggct ggagacgccg tccaaccccg gcctggacgt
     3361 ctgcgacatc gcgcgcgtcg cggacgccgc gcacgccgcc ggagcgctcg tcgccgtgga
     3421 caacaccctc gcgacgccgt tcgggcagcg cgtcctggac ctcggcgccg acctggccgt
     3481 cgccagcgac accaagtccc tgaccggcca cggcgacctg ctgctcggcc acgtcaccac
     3541 ccgtgacccg cgcctcgccg cctcgatccg gacgtggcgg aagacgatcg gcgcgatccc
     3601 cggcccgatg gaggcgtggc tcgcgcaccg ctccctggcg acgctcgcgc tccggctcga
     3661 ccgccagacc gcgaacgccc tcgccctcgc gaccgccctc cgcgaccacc cgctggtcgg
     3721 cggcctgcgt taccccggcc tgaaggacga cccggcgcac gcgctcgcgt cccggcagat
     3781 gcggcggttc ggctgcgtcg tgtcgttcac gctgccctcg cgggacgccg ccgagggctt
     3841 cctcggcgcg ttgcgcctgg tcggcgaggc gacgagcttc ggctccgtgc acagcaccgc
     3901 cgagcgccgc gcccgctggg gcggcgacgc cgtcccgccc gggttcatcc ggttctcctg
     3961 cggcatcgag gacgccggcg acctggtcgc ggacgtcctc caggccctgg acgccgtccg
     4021 ctagaccgtt tcgaggtggg cggcgaaccg ccgcagcatc tccatcagcg accggaactc
     4081 ctccggggtg aagcactcga tcatccgggc gcgctgggcg ccgatcgtgt cccggacgcg
     4141 ccggtgggcg cggacgccgt cctcggtcag ctcggccgtg tcgccgtcga ggcgcgccca
     4201 gccgaggtcg accagccgct cgacctgcgg acgcatcgtc ggctcgtccg gcgagacgaa
     4261 cgcgtccagc gtccggtcca ggtcggccat ggtgatcggc tcgtaggaca cggtgttgag
     4321 cacctgccag ccgcgccggg tgagcccgtc gtccgcgagc aggcggccga ggttcgcgtc
     4381 gagcgcgccg tcgatgtgcc gcagccagta cccgatcgga cggtccgcgc gggcgtcggg
     4441 aaaggtctcg gtcatgctct cctccaatgc atgtactttt acaatgaatg taggatgaca
     4501 tgcatgtcga gcgcaacagg tgacgaggac gggtccgccg cggacgcgag cgacgcggtg
     4561 gcggcggtcg agcgggcgat ggtggcgatc cggcgcagcc agacccgccg cacgctggga
     4621 agactcgcgg ggcccggcgg catggacccg acgctgttcg gcgtcctgga cgcgatcgag
     4681 gggcacggcg gcccgtgcgc cgtcaccgac gtcgccgccg ccctcggcgt ggaccagccg
     4741 cgcgccagcc gcctcgccct gcgcgccgag gaggagggcc tggtgacccg ggggcccgac
     4801 ccgtccgacc ggcggcgcac gctgctcgaa ctcaccgcga ccggccgcga gggcctcgac
     4861 cgcgcgcacc ggacgcggcg gaggcttttc gcgcaggtca tggccgactg gccggacacc
     4921 gaccgggacg agttcgcccg cctgatcacg gcgttcgtga cgtccttcgg cgaaacggtc
     4981 accgaaggac gcccagccgg ttagaagccg gtggggtgtg actgcgggga cgggccggtc
     5041 agcgcgacgg cggcctcggc cgtggaggcg aggaaggtga agctctcgag caggtacagg
     5101 ttcacgcccg acgcgtcgtg gccggtgtag ccgatggaga ggtcctggcc gaggctcagc
     5161 cggtagtcgc cgccccgggt gctcatcacg acgccgccgt cgatcgccgg ggcccagacg
     5221 atgccgccgt ccacgagctt ggcgaggtgc tggtagacgg gatagccctg gtcggacgag
     5281 cgggtcgcgg ccgtgtacgc ctccgacccc aggacgagcg cgtacggccc gcccacgccc
     5341 gcgagccgca gcgtggcgag ggcctcggag acgacgtccg ggtagtgcga cgcctcgacg
     5401 ggcagcggac gggccgggga cacggtgacg gcctcggcgg cgatcccggc gatgcgtccg
     5461 ggggcgtagc cgtggaagat cgcgcggtcc tcggcgaagg cgagcttggt ggccgcgtcc
     5521 ttggcgggct gccagtcggc gtcctgcgcg ccgcgctcca cggcgtccac ggcctcgcgg
     5581 gacacggtga acgggacggt cagctgcacg accggacggc tcgtgcgcag cgcggcggtg
     5641 acgccgtcgg cgggggagtc cgccgcgtcg aggtagccgg tggtgacggc cgcgagctcg
     5701 ggtccggcgg cgtccgggac gtccacgagg cggcgtccgg cgacgccgcg ggtgaaggtg
     5761 cggcggacct cgtcctccag gtcggcccag gccgccgcgg agatcggcgc gagttcgcgg
     5821 tgcaggttgt tcacggggtc tccttcagtc cgccgatgcc gagtgacccg aacaggtcgc
     5881 ccggcgcggg ctcgggtacc gcggcgggca cgggcggggc gaccgccgcg gagtcgggat
     5941 ccggcaggtc gggaggcgag tcgaggaagt cgagcgtcgg ggcgtggaac aggcacccgg
     6001 tgacggcggt ggagaagtcg aggatgcggt cggtgtgccc gtcctcggtg ccgaggaaca
     6061 tgttccgcag catctgctcg gtcgtttcgg gcgtgtgggc gtagccgatg aagtacgtcc
     6121 cgaactcgcc cttcccgggc gcgccgaacg gcatgttgtc ccgcaggatc tgccgctgct
     6181 cgccgtccgg gccgaggacg gtgttcaccg cgacgtgcga gtcgtccggc ttggtcgcgt
     6241 cgtccagctc gatgtcgttc agcttgcggc ggccgatcac cagctcctgc gcctcgaccg
     6301 gcagcgcgtt ccacgccggg aggtcgtgca ggtacttctg gacgatcacg tagctgccgt
     6361 ccgcgaactc cgggtcgtcc tcggcgcgca ccagcaccgc gcgctcggcg gcggaaccgg
     6421 cggggttctc ggtgccgtcg acgaacccca tcaggtcacg tttctcgaag taccggaacc
     6481 cgtgcacctc gtcctggacg gtcaccgcgc cgcgcagccg gtccatgatc tgcgtggcga
     6541 gctcgaagca gaggtccatc gtgcgggcgc ggacgtggaa cagcaggtcg ccgggtgtgg
     6601 ccggggcgcg gtgccggggc ccgtcgagcg cggtgaacgg atgcagcccg gccgggcggt
     6661 tcccggagaa caaccggtcc caggcgtccg agccgatgcc ggtcacgcat gtcaggttcc
     6721 cctcggggac ccggaacccg accgccctga ccagcccgga caggtcgccg agcagctcgt
     6781 gcaccaccgg ctcggccccg gcctcgatgg tgaccacgag gaacacggcg gcgtcggtga
     6841 gcgggccgag caccgcctgc gggtcgggtc gctggggttg cgcctgcggc ggcatgacga
     6901 atctcctcgg tgacgtcgct ggtgatcacg tgggccctaa gccactctct accgccccgt
     6961 gccgctcccc aaccccaagc cccacccaag acacaccgtt gtgtcgcgaa tctcctctat
     7021 cacccagtct tgccggaccg cccggacggc aggccaacgc aggtgatgcg gtgcgtcccg
     7081 ggggacgcac cgcatcacct gcgaggggct gggctcagcc cgccgcgcgc ttcagcaggt
     7141 cggcgatccg ggcctcggcg gcctcggtca gctcggtcag ggcgtaggcg gtcggccaca
     7201 tcgtgccgtc gtcgagcttc gcggtgtcgt tgaaaccgag cgtcgcgtag cgggccttga
     7261 acttgtccgc gttctggaag aagcagacga ccttgccgtc cagcgcgtag gacggcatcc
     7321 cgtaccagag cttcggctcg aggttcgggg cggcctccct gacgatggcg tggatccgct
     7381 cggccatggc gcggtcgggt tccgggaact cggcgatctt cgccaggacg tcccgctcgg
     7441 cctgcgcggc cttctccacg cgggaggcgc tgcgcccggc ggccttcagc tccttcgcgc
     7501 gctccttcat cgccgcgcgc tccccggccg agaacgtctc ggcgtcggcg tcgttcttcg
     7561 ctgcggtgga cttctcggcc atggtggttc tcctcgtctc cgtcgtcttc caggggggtg
     7621 ggggatcagc cccaggcgcc ggcgatctgc cgggtggtgg cgttgagccg gttgaacagg
     7681 ttcgtgacgc tgatcatcat gacgatcgcg gcgagctgct tctcgtcgta gtaggtggcg
     7741 gcgttgtccc agatctcgtc gctcaccgag tccggacggt cggccaggcg cgtcgcggcc
     7801 tcggccagct ccagcgccgc gcgctcctcg tcggagaagt agggggcctc gcgccacgcg
     7861 gcggtggtgg ccagcttctc ctcgctcacg cccgccttcc ggccgccctt caccccggcg
     7921 tcgacgcagg cgctgcagcc gttgatctgg ctgacgcgca ggtgcaccag ctccagggtc
     7981 tgctcgtcca cgccgccggt gtggacggcc ttgaagatgt ccaggatcgg cttcatcgcg
     8041 ccgtcgagga cctgggcggg gttctgcatc cgagcctgca tgtcgttctc cttcgtccgg
     8101 gacgttccgc ccggtttcgt cggccgctcc gtggtcggtg cggcatggct taatcacagc
     8161 cggaacgggc cgccgggacg atccccgtgg gacaccgtgg gacagctggt acggtcacga
     8221 ccagccagga ctcgaaccag gaaaagactg gcgacgcggg acagatcgac aggggtcgcg
     8281 atggtggcgg aacagcggac gacggatccg caggaggagc tggccgaacg gctccggctg
     8341 atccaggaac tgtccggacg cggcgtccgg gcgctcgcgc gggacaccgg cctcagctcg
     8401 tcctcgctgt cgcggtacct cggcgggcag accgtcccgc cgtggcccgc ggtcgtcgag
     8461 ctgtgccgcc tggtgaagcg cgacccgcgt ccgctgcgtc cgctgtggga gcgggccggg
     8521 agcccgctgc ccgcgccgcc gaagaccagc cgccaggtcc agccgccgtc gccgcccgcc
     8581 gggacgccgc gtccgccgcg caacgacctg ccccgcgacg tgcccgactt caccggacgc
     8641 gaggacggcc tcgccgccgt gctcgccgcc gtcgaggccg accggctggt cgcgatcgac
     8701 ggcatgccgg gcgtcggcaa gacctgcctc gccgtccacg cggcgcaccg gctcacgacc
     8761 ggctacccgg acgcgcagct ctacctcgac ctgcacgggt tcaccgaggg acgcgagccg
     8821 ctcgaccccg acatggcgct gcggacgctg ctcgccgcgc tggacgtccc gtccgagcgg
     8881 atcccgcagg cgggcggcat cgagcccctc gccgcgtgct ggcgcgcgga gctggcgtcc
     8941 cggcgcgcgg tcgtcgtcct ggacaacgcc gcgggggcgg accaggtccg ccacctgctg
     9001 cccggcgcgg gcccgtcggt cgcgctgatc accagccgca accgcctgct cggcctggac
     9061 gaggtcccgc cggtgtcgtt ggacgtcctg tcccccgagg agagcgccga gctgctggcc
     9121 cgcgcgagcg gcgagtccgg ccgtccggag ggacgcctgg cccgcgagcc cgagcaggcc
     9181 gccgaggtgc tgcgcctgtg cggccacctg ccgctcgcgc tgcgcctggc cgccgcccgc
     9241 ctccggcacc gtccgggctg gaccgtcggc atcctcgtcg agcggatggc ggagggcccg
     9301 agtgagttcg acacggcgtt cgcgatgtcg gtccggcagc tggaccggtc gcagcgccgc
     9361 ttcttccgcc tcctcgggct gctgcccggc tcgtccttcg acgagtacgt ggcggcggcc
     9421 gtcgcggacg tccccctgcg cgccgcgagg acgatgctgg aggacctcct cgacgcgcac
     9481 ctcgtccagc agccgtccgg gggtcgctac cggctgcacg acctcgtccg ggagcacgcc
     9541 cgccgcgcca ccgccgagca ggacacccag gacgaccgcg accgggccct cggacgcgtc
     9601 ctcgactact acgtccacgc ggcggcagcc gccgagtccg cgatgccgta cctgtcccgc
     9661 agccgggcgc cgaccgcggg cgaggccccg gccgagctcc cggccttcgg ccacaagcac
     9721 caggccctct tctggttcgg caccgagtac acgaacctga tgaccgtctt cgacacggcc
     9781 gtcgcgaccg gagccgacgt ccacgcctgc gaactgcccc gcttcatgcg ggcgttcttc
     9841 gcccgccgct gcgggaccac gcacctcaac gtcctgttcg aacgcgccct ggacgccgcc
     9901 cgccgcctgg acgacccgct ccggctggcc gaagcccaca gcgacctcgg cttcgcccgc
     9961 tacaacgcgg gccgcatggc cgaggcccac gccgcctacg acaccgccgc gcccctgatc
    10021 gccgggaccg gcgacacctg ggccgaggcc gagctggcca tgcgccgcgc cttcctcctc
    10081 tgggacgagg gcaacgtgca ggagcccctg accctcttct gccgggccgc cgcgctctac
    10141 gactccatcg gctcgctcaa gggcagggcc cacgcgaccg ccaacgaggc ctgggcgctc
    10201 ctccagctcg gctaccgcga ggacgccgcc gcccgggccc gccaggccct cgacctgacc
    10261 ggcgacgagc ccgactggcc gtccacgctg acggcccgca tcaccctcgg cgtcgccctc
    10321 gcccccgaaa atcccgacga ggcgaacgac cacctcaccc gggccctgga actggcccgc
    10381 gaggacagcc acaagcacaa cgaggcctgg tgcctcaact gcctgggcgt cgccctgcgc
    10441 cagatgggcc gctacgacga ggccctcgcc agccaccgcc gggccttcgc cctcctggac
    10501 gagctcttcg aggagcactg gaagatccac ttcctcaacg gctacggcga gacctgccgc
    10561 ctggcgggtc tgcccgacga ggctctccgc ctccaccgcc aggccctgga gctggccccc
    10621 gaggtgggct accgccacga ggaggccctc gcccacgagg gcatcgccgc cgtcctggcc
    10681 gaaaccgacc ccaacaccgc cgccgagcac cgcgccgccg ggctcgccct cctccaaacc
    10741 tcctgagacg gcgagaaatg atcagaagca ggaccgtcgt cgtcgtgggg gccctggcgg
    10801 cggccgggtt cctcgtctcc ccggtcgtcc ggtc
//
