LOCUS       NZ_JADG01000010        10993 bp    DNA     linear   CON 02-MAY-2024
DEFINITION  Actinomadura oligospora ATCC 43269 P696DRAFT_scaffold00008.8_C,
            whole genome shotgun sequence.
ACCESSION   NZ_JADG01000010
VERSION     NZ_JADG01000010.1
KEYWORDS    WGS; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Actinomadura oligospora ATCC 43269
  ORGANISM  Actinomadura oligospora ATCC 43269
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Thermomonosporaceae; Actinomadura.
REFERENCE   1  (bases 1 to 441953)
  AUTHORS   Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K.,
            Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A.,
            Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2014) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            environment                       :: Soil
            investigation_type                :: bacteria_archaea
            project_name :: Actinomadura oligospora ATCC 43269
            sequencing_meth                   :: WGS
            ref_biomaterial                   :: ATCC 43269
            GOLD Stamp ID                     :: Gi22378
            Type Strain                       :: Yes
            Isolation Site                    :: soil, India
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Unknown program v. before 2013-09-10
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000518265.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 02:24:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,332
            CDSs (total)                      :: 8,253
            Genes (coding)                    :: 8,185
            CDSs (with protein)               :: 8,185
            Genes (RNA)                       :: 79
            rRNAs                             :: 7, 2 (5S, 16S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 7 of 68
            Pseudo Genes (incomplete)         :: 65 of 68
            Pseudo Genes (internal stop)      :: 1 of 68
            Pseudo Genes (multiple problems)  :: 5 of 68
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:28:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 285456
            Orig. end                         :: 296449
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JADG01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
FEATURES             Location/Qualifiers
     region          1..10993
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10993
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10993
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5993](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5993
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(411..427)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.69"
                     /tool="antismash"
     misc_feature    complement(479..494)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.09"
                     /tool="antismash"
     misc_feature    complement(497..512)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     misc_feature    586..597
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.93"
                     /tool="antismash"
     misc_feature    686..704
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 19.13"
                     /tool="antismash"
     misc_feature    699..717
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.63"
                     /tool="antismash"
     gene            complement(848..2017)
                     /locus_tag="P696_RS58765"
     CDS             complement(848..2017)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /locus_tag="P696_RS58765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="M28 family peptidase"
                     /protein_id="WP_281174953.1"
                     /transl_table=11
                     /translation="MRRSPPPATTRPSSRSPTAVSTSRPSGPTCSPPPPRCAPSCWTSR
                     RRSLRAASPHGSSSARRPAARPRTTTAWTSATPSSSSAARPVATASSSTSPPARERAPC
                     CSTSPPRPRRTSGGCTGSGPRRRRSRWPPSRSSRADWLRAQAASGPLTLNLDWRGRTVS
                     GVTNNLIAETTSGDKDRVVIAGGHLDSVGEGPGINDNGSTAAALLQTALKLAPMQHTAT
                     NRVRFIWWGAEELVDVGSDYYVDQLTPAQKQSILLYLNAEMIGAPNFVSFVMNGPGATS
                     VPFDAYFTQRHLPYELAPTTAVGSDHEPFMKAGIPVGGMFGGSVGAKTADQAARFGGIA
                     GQAYDPCYHQPCDTTANINPLALDRHSRAFAYAVGWAATTPTITVPQTP"
     misc_feature    complement(2173..2187)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     gene            complement(2390..2932)
                     /locus_tag="P696_RS46275"
     CDS             complement(2390..2932)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /locus_tag="P696_RS46275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_034485358.1"
                     /sec_met_domain="p450 (E-value: 1.9e-17, bitscore: 57.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAILLVLAGHETTVNLVGMGVMTLLLHPGQLAVLRADAALLPSAV
                     EELIRHTGPIGTGLARFATTDLDLAGTRVRRGDLVVTSLAAANRAPARFTDPHRFDVRR
                     GEAPHVGFGHGIHFCLGAALARLEAEVAIGTTLRRLPDLALAKPAERLEWRTSSLRGLA
                     ELPVTFTPSPPLARPPH"
     gene            complement(2866..3642)
                     /locus_tag="P696_RS46280"
     CDS             complement(2866..3642)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="P696_RS46280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="MFS transporter"
                     /protein_id="WP_026413335.1"
                     /transl_table=11
                     /translation="MLRLGTRERRPDTGARPPLLRDSLSGLGEVMRFRPVGQILVIGWI
                     LPMLASAPEALVIPYAHELHVGKVQTGILMGALPAGFVLGEVLMTWLGSAALQVRLVPV
                     LTTVMFVPLLVYVTRPGLLVTLVVLVVSGIGGAQHLGLDRLLLASAPEPLRARALGLQT
                     AGLMFWQGTGFALSGAVAEFVPLHLVVFAASVTGVIAAFLTTRGLPRTRRTTPLSTRFV
                     SVHAFRTCPRVSSLAWRSCSSWPVTRPPSTSSAWAS"
     gene            complement(3672..4001)
                     /locus_tag="P696_RS52595"
     CDS             complement(3672..4001)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="P696_RS52595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157429753.1"
                     /transl_table=11
                     /translation="MTVASVQQSRYRDALAVGEFRAIFGSFVISMLGTVVAELALTVLA
                     FVLGLIAPVFASTRAATIADALPEAAYAPGRSLLRVFTQGAQIGGFAIGGARCSRSSPR
                     AARCC"
     misc_feature    4018..4031
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 20.06"
                     /tool="antismash"
     misc_feature    4027..4041
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.76"
                     /tool="antismash"
     misc_feature    4255..4269
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.57"
                     /tool="antismash"
     gene            4438..5004
                     /locus_tag="P696_RS58150"
     CDS             4438..5004
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="P696_RS58150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_051467798.1"
                     /transl_table=11
                     /translation="MAKDKGQDGHDGRLTAKDIEAFVKGGENGLQQRHRVQESGPERGA
                     AAQETEATDLHVCMGLNACVGHGAKGSGMMAGMGECATVTHVCHGANECRGQGGCGYSG
                     HDAEQAIPGAQHCRYSGSCASPINESRVHAAGPFRGTSVWKRARKVFEQRMYEAGVPFG
                     PSPGAGIPDDLVPPYERPRPHGTPQ"
     gene            5001..5993
                     /locus_tag="P696_RS46290"
     CDS             5001..5993
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619267.1"
                     /locus_tag="P696_RS46290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_064744051.1"
                     /sec_met_domain="DUF692 (E-value: 1.7e-99, bitscore: 327.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGPRPERDATGLPNLGLGLGLRSPHLAYVLAEWPQVDWFEIISE
                     NFMDSGGYPRHALDRIAERYPIVMHGVSLSIGGSDPLDMDYLARLRALADSLPAAWVSD
                     HVCWTGVAGLNTHELLPIPFTEESLRHVARRVEVVQDVLQRPLVLENPSTYASFAHSAM
                     TEWEFLGRLCEESGCLLLLDVNNVVVSAFNHGFDPDEYVRALPHDRIAQIHLAGHADHG
                     THRIDTHDRPVPDSVWDLYGRAIALAGPTPTLLEWDDRLPAFPDLLAELAKARPYVEAR
                     HPATDGAPAASGVAASGGAPAFGGVTAAGGVTVAGGVTVAGGVTAATHG"
     gene            5986..6930
                     /locus_tag="P696_RS55975"
     CDS             5986..6930
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /locus_tag="P696_RS55975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_026413337.1"
                     /transl_table=11
                     /translation="MGDAADAPTLTDVQHRLQYALLDPGAPAEQAEAMLAGTPELSAAR
                     RLEIYRNGCRNRQLETMAHLHPALRHLLGDELFDDFASGYLEARPSRAYTMARLDEGFA
                     GYLSAGRPDSDGSQAWVDILIDLARYERAFAEVYDGPGTEQDGPLTDLLTGQVPEWPAG
                     RPALAAALGERRIVMAPCVRSLRVGAAVHEYHAAVRGGTNPPPLTPQPTYLALWRRDYR
                     VRVTALAPQAHALLEALLGGAAFREARATAALEPAVAWGHVREWSACGWISAFTEPPPP
                     PQTPETSETPATPGARREPAASSTPPAPHGSTQ"
     gene            6927..8165
                     /locus_tag="P696_RS0117505"
     CDS             6927..8165
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /locus_tag="P696_RS0117505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ferritin-like protein"
                     /protein_id="WP_026413338.1"
                     /transl_table=11
                     /translation="MSTQTNIEQPAVTLSTILDEFPVDARKGIKTLEQLVNHLLQAAAV
                     EMQTIPMYLYAMFSLSGRGNSRWDPGIGAQRLIRSIVVEEMLHLCMVRNILVALGQGGK
                     AKFYNKQFIPDYPELMLHRHPDLVLHLGKCDTELVRTVFMEFERPKAKPGEGKPPKGQY
                     ATIGLFYKSIMKGLEYLDGKQNLWGNTQRELQYMGAYWNKDGGGEPFVVENLKTATQAL
                     KMIVDQGEGVDPKKPSVPIDPLNPKPGLDELPHYTKFQRIADGIEPIGPVWVVDTDPKS
                     LQYLEDPAASSINDLFNAAYCYVLHMIDVIYSTSAEDIAPGARSRRYGLERSFMAAMQG
                     VLSGIAEAMVARTLAPGGLDDPQIAPTFEYVDLPATGKKKHLIDLCNKAIPHFPQLGGD
                     NSTLWLLNKMPDI"
     gene            8280..9185
                     /locus_tag="P696_RS0117510"
     CDS             8280..9185
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013155909.1"
                     /locus_tag="P696_RS0117510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_026413339.1"
                     /transl_table=11
                     /translation="MAIIEVDGLRKSYDGRTVLDGVSFAVEEGEIFGLLGPNGAGKTTT
                     VECVEGLRRPDSGSVRVAGLDPAADRKGVTRILGVQLQHSDLQDRLTVGEALGLYAAFY
                     PHPADGRALAGRLGLTDLLGHRFGTLSGGQKQRLFIALALIGRPRVVVLDELTTGLDPN
                     ARRDTWELIDGLRNDGVTILLVTHFMEEAQRLCDRVAVLRSGVLAALDSPARLIEHARS
                     ATIVSFTPSRPPDDDALAALPALRTVRRPGDGRLVVSGTDATVEALMSLLARDGIVAHN
                     LRVETATLDDAYLDLTRGEH"
     gene            9187..9927
                     /locus_tag="P696_RS0117515"
     CDS             9187..9927
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1065: ABC-2 type
                     transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656636.1"
                     /locus_tag="P696_RS0117515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_026413340.1"
                     /transl_table=11
                     /translation="MSASVAVLRAEARLLSREPASVFWILAFPGLLLTLVGLVPSFRAD
                     APAPGGGRTIDLYVPVTVLLGLVMAGLQSLPPTLAGYREKGILRRMSTTPVRPAALLAA
                     QVIVHGAAALAGGIVVVAAGRLLYGVRLPANPAAYTATLALATLTALVAGALVAALSRT
                     AKGSTTTATILFFPVAFTAGVYVPVQSMSEDLRRVVELTPFGAASQALHQAASGGWPNP
                     SHLAIMLVWTVVLGGVSARWFRWE"
     misc_feature    complement(10537..10552)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.11"
                     /tool="antismash"
ORIGIN
        1 acttgacgtg gaccacaacc gagctggtct tgtaggtgtg gttccagtgg tggtggcggt
       61 ccctggactg gacgacgcca cggaaggtgt aggcgccctt cttcgttccg aagcggtacc
      121 cgagggtgta gacctgcgcc ttgtccttgc ggctccagtg gctttgggtc gtgcacttca
      181 cggtcgtgaa cttcgccttg ccggccgagc gctggaggca gaagcgcagg ccgctgaggt
      241 gctccccggc cgagtccgag gccgccgcca tctggaacgc gaccgcgccg cgggggtgga
      301 cggtcttgtg tcccgccgtc agggtgaagc tttccttcgc cgaggccgcc ccggccgtgc
      361 ccagcgtgac cgtcccggcg gtcgcgagga cgcccgcggt gaggatgagc ttgcgtcgca
      421 tcagcaactc ctgtctcgtt tcttggtgga cgagacgtta gatgccacgc ttacgcaaga
      481 ggttaaacat caatcatgtg acgtgtgtca catgtcagcc ggccattttc ggagggcgtg
      541 atcgaggccc gtgaccggtc catatcaacg tcacgtatgg acactgaaaa cgttacgcta
      601 acctgcgcct gaaggaacct cgcgccggtc ggcgaacatg cgggcgctgg agaattcgac
      661 atcccctcga agcttcctcc gcgcgacggt gtgtaccgaa tgcgcgtgcc tcgcagtggc
      721 cattcctata tcggtcaatc agtgaattcg aagcggacga tcggtgccgc gagtggtgga
      781 cggcgacggc ggcgcgtgca cggaggggtt ccgtgcgcgc gccgccgtcg ttgggtgggc
      841 gtggtggtca gggggtctgg gggacggtga tggtgggggt ggtggccgcc cagccgacgg
      901 cgtaggcgaa ggcgcggctg tggcggtcga gggcgagcgg gttgatgttg gcggtggtgt
      961 cgcagggctg gtggtagcag gggtcgtagg cctggccggc gatgccgccg aaccgggcgg
     1021 cctggtcagc ggtcttggct ccgaccgagc ctccgaacat gccgccgacc gggatcccgg
     1081 ccttcatgaa cggctcgtgg tccgagccga ccgcggtggt gggggccagc tcgtacggca
     1141 ggtggcgctg ggtgaagtag gcgtcgaacg ggaccgaggt cgctccgggg ccgttcatga
     1201 cgaagctgac gaagttgggc gcgccgatca tctcggcgtt gaggtacagc aggatggact
     1261 gcttctgcgc gggggtgagc tggtcgacgt agtagtccga ccccacgtcc accagttcct
     1321 cggcgcccca ccagatgaac cgcacgcggt tggtggcggt gtgctgcatc ggcgcgagct
     1381 tgagcgcggt ctgcaggagg gcggccgcgg tggagccgtt gtcgttgatg ccggggccct
     1441 cgcccacgct gtcgagatgg ccgccggcga tcaccacacg gtccttgtcg ccgctcgtcg
     1501 tctcggcgat caggttgttg gtgacgccgg agacggtccg tccgcgccag tcgaggttga
     1561 gggtgagcgg accggacgcg gcctgggccc gaagccagtc ggccctgctg ctgcgagatg
     1621 gaggccagcg ggatcggcgg cggcgcggcc ccgaaccagt gcagccgcca gacgtcctgc
     1681 ggggtcgtgg tggggatgta gagcagcacg gcgcgcgctc ccgcgccggc ggcgacgttg
     1741 agctgctggc cgtggccaca ggacgcgcgg ccgaggagga cgacggcgtc gcggacgtcc
     1801 aggccgtcgt agtccgcggc ctggcagccg gacgccgggc cgatgacgag ccgtgtggag
     1861 acgccgccct cagggagcgg cggcgactgg tccagcagga gggtgcgcag cgaggtggcg
     1921 ggggactgca cgtcggtccg ctcggcctgg acgtcgaaac ggctgtaggg gaccggctgc
     1981 ttggtcgggt ggtagccggc ggcggtgagc gccgcatcac gtagtcctcg gacgcggtga
     2041 ccccccgggt gccgaccgcg cggttgccgt tgttggcgtc ggcgatgcgc tggaaggcgt
     2101 ccaggtgccg ggtgacggcg tcgcccgtca ccgcggtggc cagccggtcg gcgaggccgc
     2161 cgcgtgaggg ggtggccgag gcgctcgggc cgacagggcg atcacgctcg cgagcgccac
     2221 gggggcgtcg agcgcggcgg atcggaggag gcggctcggc agtgggcggc gggtgcggct
     2281 cttcggggat gacatgggtg ctctccgaga ggtgtggtcg ggcgcggaca aggtgggtgc
     2341 gggcgtgcgg gtgtgctccg tccgatctgg cggacgggtg tgcgggcgct cagtgaggtg
     2401 gacgggcgag gggaggggac ggggtgaagg tcacggggag ctcggcgagg ccgcgcaggg
     2461 acgaggtgcg ccattccagt cgttcggcgg gcttggccag agccaggtcc gggaggcggc
     2521 ggagggtggt gccgatggcc acctcggcct cgaggcgggc gagcgcggcc ccgaggcaga
     2581 agtggatgcc gtgtccgaac ccgacgtgcg gcgcctcgcc gcgccggacg tcgaaccggt
     2641 gcgggtcggt gaaccgggcg ggggcccggt tggcggcggc cagcgaggtg acgacgaggt
     2701 ccccccggcg gacgcgcgtg cccgcgaggt cgaggtcggt ggtcgcgaag cgggccagtc
     2761 ccgtgccgat cggcccggtg tggcggatca gctcctcgac ggcggacggc agaagcgcgg
     2821 cgtcggcgcg caggacggcg agctgcccgg ggtggagcag caacgtcatg acgcccatgc
     2881 cgacgaggtt gacggtggtc tcgtgaccgg ccaggacgag caggatcgcc atgccagaga
     2941 cgaaacgcgt gggcaggtac gaaacgcgtg gacagagacg aaacgcgtgg acaggggcgt
     3001 cgtgcgccgg gtgcggggga ggccccgggt ggtgaggaaa gccgcgatca cgccggtcac
     3061 cgaggccgcg aacacgacca ggtgcagcgg gacgaactcg gcgacggccc ccgacaacgc
     3121 gaaccccgtc ccctgccaga acatcagccc ggcggtctgg agaccgagcg cgcgggcgcg
     3181 cagcggttcc ggggcgctgg ccaggagcaa ccggtccagc ccgaggtgct gggccccgcc
     3241 gatccccgac accaccagga cgaccagagt gacgagcagg ccagggcgcg tcacgtacac
     3301 cagcagcggc acgaacatca ccgtggtgag caccggcacc agcctcacct gcagagccgc
     3361 gctgccgagc cacgtcatca gcacctcgcc gagcacgaac cccgcgggca gcgcgcccat
     3421 gagaatcccg gtctggacct tccccacgtg gagctcgtgc gcgtacggga tcaccagcgc
     3481 ctcgggcgcg ctcgccagca tcggcagtat ccagccgatg accaggatct ggccgaccgg
     3541 ccggaacctc atgacctccc cgagccccga cagcgagtcc cgcagcaggg gcggacgggc
     3601 gccggtgtcg gggcggcgct ccctggtccc cagccgcagc acgaccgccg agcccgcgaa
     3661 gcacgccgcg ttcagcagca gcgcgccgct cggggagatg accgtgagca gcgcgcaccg
     3721 ccgatcgcga agcctccgat ctgcgcgccc tgggtgaaga cgcgcagcag gctccgcccc
     3781 ggcgcgtagg ccgcctcggg cagcgcgtcc gcgatcgtgg ccgcccgcgt gctcgcgaag
     3841 accggcgcga tgagtcccag gacgaacgcc agcaccgtca gggccagctc ggcgaccacc
     3901 gtgcccagca tcgagatcac gaacgacccg aagatcgcgc ggaactcccc gacggccaac
     3961 gcgtcccggt aacgactctg ctgaaccgac gccacagtca tgacatgaac gtcgcacgcg
     4021 accccgatca aggccaacga ttcgtcccac gacgaatcag cccgaggaca ccgcgacggg
     4081 gggtcttcca ttcggcagga tctgctgtgc cgccccttcg gaattcgcca cgagtgcccg
     4141 ttgacggatg accggcgtcc atgacattcc cggtggcctg atggccgacg gcggagccgt
     4201 caccggcttc gcccgcgacg gttggggtaa tttgccctga tatatggcga acagtggcaa
     4261 ttgtttgcag gcgtccggag tgaagtgtcc gcttctcgcg gaaaggtgag ggaaatgctc
     4321 ccttgtttcg tctggcgttt gtccgcgaga tgctccggga agcatggacg tggtcgatcc
     4381 gccggtcgtt ccgggcggtg ccggaaggct cgaattcaca ggacgaggtt gcttgccatg
     4441 gcgaaggaca aaggccagga cggtcacgac ggacggctga cggcgaagga catcgaggcg
     4501 ttcgtcaagg gcggtgagaa cggccttcag cagcggcacc gggtccagga gtccgggccc
     4561 gagcgcgggg ccgcggccca ggagacggag gcgacggacc tgcacgtgtg catggggctg
     4621 aacgcctgcg tcgggcacgg cgcgaagggc agcgggatga tggccgggat gggggagtgc
     4681 gccacggtca cccacgtgtg ccacggcgcc aacgaatgcc gcggccaggg agggtgcggc
     4741 tacagcgggc acgacgccga gcaggccatc cccggggcgc agcactgccg gtactcgggc
     4801 agctgcgcga gcccgatcaa cgagagccgg gtccacgcgg cggggccctt ccgcgggacg
     4861 agcgtgtgga agcgggcccg caaggtgttc gagcagcgca tgtacgaggc cggggtcccg
     4921 ttcgggccgt cccccggcgc ggggatcccc gacgacctgg ttccgcccta cgagcgtccg
     4981 cggccccacg gcacgcccca gtgaccggcc cgaggcccga acgggacgcg accggcctgc
     5041 cgaatctggg gctgggcctg gggctgaggt ccccccacct ggcgtacgtc ctcgccgagt
     5101 ggccccaggt cgactggttc gagatcatct ccgagaactt catggactcc ggcggctatc
     5161 cccggcacgc gctggaccgg atcgccgagc ggtatccgat cgtcatgcac ggggtgtccc
     5221 tgtcgatcgg cggcagcgac cccctcgaca tggactacct ggcgcggctg cgcgccctgg
     5281 ccgactccct tccggcggcc tgggtctccg accacgtgtg ctggacgggc gtcgccgggc
     5341 tgaacaccca cgagctgctg ccgatcccgt tcaccgagga gtcgctgcgt cacgtcgcgc
     5401 gccgtgtcga ggtcgtccag gacgtcctcc agcggccgct ggtcctggag aaccccagca
     5461 cctacgcctc gttcgcccat tcggcgatga cggagtggga gttcctgggc cgcctgtgcg
     5521 aggagtcggg ctgcctgctc ctgctcgacg tcaacaacgt cgtcgtgtcg gccttcaacc
     5581 acggcttcga cccggacgag tacgtgcggg cgctgccgca cgaccggatc gcgcagatcc
     5641 atctcgcggg ccacgccgac cacggcaccc atcggatcga cacccacgat cgcccggttc
     5701 ccgacagcgt gtgggacctg tacggccggg cgatcgccct ggcgggcccg acccccacgc
     5761 tcctggagtg ggacgacagg ctcccggcgt ttcccgacct gctcgcggaa ctggccaagg
     5821 cacgtcccta cgtcgaagcg cgccacccgg cgacggacgg cgccccagcg gcgagcggcg
     5881 tcgcggcatc aggtggcgcc ccagcgttcg gcggcgtcac ggcggcgggc ggcgtcacgg
     5941 tggcgggcgg cgtcacggtg gcgggcgggg tcacggcggc cacccatggg tgatgcggcg
     6001 gacgcgccga cgctcaccga cgtccagcac cgcctgcagt acgccctcct cgaccccggg
     6061 gcaccggctg agcaggccga agcgatgctg gcggggacgc cggagctgag cgcggcgcga
     6121 cgcctggaga tctaccggaa cggctgccgg aaccgtcagc tggagacgat ggcgcacctg
     6181 catcctgcgc tgcgccatct cctcggggac gaactgttcg acgacttcgc gtccgggtac
     6241 ctggaggccc gtccgtcgcg tgcctacacg atggcgcgcc tggacgaggg gttcgccggc
     6301 tacctgtcgg ccggacggcc ggactccgac ggctcgcagg catgggtcga catcctcatc
     6361 gacctcgccc gctacgaacg tgccttcgcc gaggtctacg acggcccggg aaccgagcag
     6421 gacggcccac tcaccgacct gctcaccggc caggtcccgg agtggcctgc cggacggccc
     6481 gccctcgcgg cggccctcgg cgagcggcgg atcgtcatgg cgccctgcgt gcgctcgctg
     6541 cgggtcggcg ccgccgtcca cgagtaccac gcggccgtgc gcggcgggac gaacccgcca
     6601 ccgctcaccc cacagccgac atatctggcc ctctggcgcc gcgattaccg cgtccgggtg
     6661 accgctctcg ctccgcaggc ccacgctctg ctcgaagccc tgctcggcgg ggccgccttc
     6721 cgcgaagccc gcgccacggc ggccctggaa ccggccgtgg cctgggggca tgtgcgcgaa
     6781 tggtccgcgt gcggctggat cagcgcgttc accgagcccc cgccaccacc gcagacaccg
     6841 gagacatcgg aaacgcctgc gacgccgggg gcgcggcgcg agcccgccgc gtcgtccaca
     6901 ccaccagcac cacacgggag cacgcagtga gcacgcagac gaacatcgag cagcccgccg
     6961 tcacgctgtc caccatcctg gacgagttcc cggtcgacgc ccgcaagggg atcaagaccc
     7021 tggagcagct cgtcaaccac ctgctccagg ccgcggccgt ggagatgcag accatcccga
     7081 tgtacctgta cgccatgttc tccctgtccg ggcgtggcaa ctcgcgatgg gaccccggga
     7141 tcggcgcgca gcggctcatc cgcagcatcg tcgtcgagga gatgctgcac ctgtgcatgg
     7201 tccgcaacat cctcgtagcc ctcgggcagg gcgggaaggc gaagttctac aacaagcagt
     7261 tcatccccga ctatcccgaa ctgatgctgc accgccaccc ggatctggtg ctgcatctcg
     7321 gcaagtgcga caccgaactc gtacggacgg tgttcatgga gttcgagcgt cccaaggcca
     7381 agccgggtga ggggaagccg ccgaaggggc agtacgccac gatcggcctc ttctacaagt
     7441 ccatcatgaa gggcctggaa tacctggacg gaaagcagaa tctctggggc aacacccagc
     7501 gcgagcttca gtacatgggg gcgtactgga acaaggacgg tggcggggag cccttcgtcg
     7561 tcgagaacct caagaccgcc acccaggcgc tgaagatgat cgtcgaccag ggcgaggggg
     7621 tcgaccccaa gaagccgtcg gtgcccatcg acccgctcaa ccccaagccg ggactcgacg
     7681 aactcccgca ctacaccaag ttccagcgga tcgcggacgg gatcgagccg atcggcccgg
     7741 tctgggtcgt ggacaccgac cccaagtccc tccagtacct cgaggaccct gcggcgtcca
     7801 gcatcaacga cctgttcaac gccgcctact gctacgtgct ccacatgatc gacgtcatct
     7861 acagcacctc ggccgaggac atcgccccag gcgccaggag caggcgctac ggcctcgaac
     7921 gctccttcat ggccgccatg cagggcgtcc tgagcggcat cgccgaggcg atggtcgccc
     7981 gcaccctcgc tcccggaggt ctggacgatc cgcagatcgc tccgaccttc gagtacgtcg
     8041 acctccccgc cacgggcaag aagaagcacc tcatcgacct gtgcaacaag gcgattcccc
     8101 acttccccca gctcggcggc gacaacagca ccctgtggct gctcaacaag atgcccgaca
     8161 tctgaccgag ggggcagcga agacaacggg gtcgtaggac ggcatcagcc gatcggctga
     8221 tgcggcttcc agcgtgacgg acgatgcccg gcgggggagc ggcgcgggag gctccccgca
     8281 tggcgatcat cgaggtcgac gggctgcgga agtcctacga cggccggacg gtgctggacg
     8341 gggtctcctt cgccgtcgag gagggcgaga tcttcggact gctcggcccc aacggcgcgg
     8401 gcaagaccac cacggtcgag tgcgtcgagg ggctgcgcag gcccgactcc ggcagtgtgc
     8461 gggtcgccgg cctggacccg gccgccgacc ggaagggcgt cacgcggatc ctgggcgtcc
     8521 agctccagca cagcgacctg caggacaggc tcaccgtcgg cgaggcgctg gggctctacg
     8581 ccgccttcta cccgcatccg gcggacgggc gggccctggc cggacggctc ggcctgaccg
     8641 acctgctcgg ccaccgcttc gggacgctgt ccggcgggca gaagcagcgc ctgttcatcg
     8701 cgctcgcgct gatcggccgt ccgagggtgg tggtcctgga cgagctgacc accggcctgg
     8761 acccgaacgc gcgccgcgac acctgggagc tgatcgacgg cctccggaac gacggcgtga
     8821 cgatcctgct cgtcacccac ttcatggaag aggcgcagcg gctgtgcgac cgggtcgcgg
     8881 tgctccgctc gggcgtcctg gccgcgctgg acagccccgc ccggctgatc gagcacgcca
     8941 ggagcgcgac gatcgtgtcg ttcaccccct cgcggccgcc cgacgacgac gcgctggccg
     9001 cgctgcccgc cctccggacc gtccggcgtc cgggcgacgg gcggctggtc gtcagcggga
     9061 cggacgcgac cgtcgaggcg ctgatgtccc tgctcgcgcg cgacgggatc gtcgcgcaca
     9121 acctccgggt ggagaccgcc accctcgacg acgcctatct cgacctcacc cgcggggagc
     9181 actgacatgt ccgcgtccgt cgccgtcctc agggccgagg cccggctgct gtcccgcgaa
     9241 ccggccagcg tgttctggat cctcgccttc cccggcctgc tgctgacgct ggtcggcctc
     9301 gtcccgtcct tccgggccga cgccccggct cccggcgggg gacggaccat cgacctgtac
     9361 gtcccggtca ccgtgctgct cggactggtc atggccggtc tccagtcgct gccgccgacc
     9421 ctggcgggct accgcgagaa ggggatcctg cgccggatgt ccaccacgcc ggtccgtccg
     9481 gccgcgctcc tggccgccca ggtgatcgtg cacggcgccg ccgccctggc cggcgggatc
     9541 gtcgtcgtcg cggccggccg cctgctgtac ggcgtccgcc tcccggccaa tcccgccgcc
     9601 tacacggcga cgctggccct cgccacgctc accgcgctgg tcgcgggggc gctcgtcgcc
     9661 gcgctgtcgc ggacggccaa gggctccacg accacggcca cgatcctctt cttccccgtc
     9721 gcgttcaccg ccggggtcta cgtcccggtc cagtccatgt cggaggacct gcggcgcgtc
     9781 gtggaactca ccccgttcgg cgccgcgtcc caggccctcc accaggccgc ctccgggggt
     9841 tggccgaacc cgtcccacct ggcgatcatg ctggtgtgga ccgtggtgct gggcggagtc
     9901 tcggcccgct ggttccgctg ggaatgatcc aggctcgggg ggacggcaca ttgaacgcga
     9961 cgccggatgc gacgctggac ggcacgctgg acgcgactgc ggacggagcc acgacggacg
    10021 cgaccgcggc ggacgggacc gcggcggacg gggccgcggc ggtcgcgact gcggacgggg
    10081 ctgtgacgga cggggccgcg tcgtggcagc ggcggcttct gaacggcggg ccgttcgtgc
    10141 tgctgggggt gggcggggtg ctcgcgaccg tcagcgcgga cgcgctgatg acgcccggca
    10201 gggagcgcgc cgccatcgtc ctcgcggtgg ccgcgctcgc ccttcaggcg tggtggagcc
    10261 tcgtgggcgg gcggcggcgt ccacgtggtc cggcgcatcg cgtctactac ctgctgcggt
    10321 gggcggccgg gttcgcgctc acctggtgca atcccctgtt cggggtctac gcgctgctcg
    10381 gctactacga cgcgggcagg ctgctgccgc gccacctggt gcgtcccgcg ctgttcgcca
    10441 cggcggtcac catggcgggt tccaacgcgg gcggtctgcc gcccacggag ggcaggcact
    10501 ggctggcgtt cgtcgcgctg ctggccctgc acgccgggct cgccctcggc ttcgaccaca
    10561 tcgcggtcaa ggacgccgag caggccgcgt cgagggcggc ggccctggcc gaggcgcggc
    10621 tggcgaacgt ccgcctcgcc cgggcgctcc aggagaacgc gggcctgcac gcccagcttc
    10681 tcgtccaggc cagggaggcg ggcgtcgccg acgaacgccg gcggctggcg gccgagatcc
    10741 acgacacgct cgcccagggg ctgaccggga tcgtcaccca gcttcaggcg gcggccgaca
    10801 gcgccgaccc ggccacgacg cgcgaccacg tccagcgcgc gtccgcgctc gcccggcaca
    10861 gcctgggtga ggcccgccgg tccgtccagg acctcggccc cgcgcagctg gagcaggatc
    10921 ccctgcccac ggcgctggag aaggtcgtca agggctggtc ggacgagacg ggcgtccagg
    10981 gcgagttcgt cgt
//
