LOCUS       NZ_AZXF01000050         2851 bp    DNA     linear   CON 21-OCT-2024
DEFINITION  Nocardiopsis sp. CNT312 B042DRAFT_scaffold_44.45_C, whole genome
            shotgun sequence.
ACCESSION   NZ_AZXF01000050
VERSION     NZ_AZXF01000050.1
KEYWORDS    WGS; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Nocardiopsis sp. CNT312
  ORGANISM  Nocardiopsis sp. CNT312
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsidaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Jensen,P., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            assembly :: Velvet v. 1.1.04; ALLPATHS v. R41043
            investigation_type                :: bacteria_archaea
            project_name                      :: Nocardiopsis sp. CNT312
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi14643
            Funding Program                   :: DOE-CSP-ICBG 2011
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. R41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000515115.1-RS_2024_10_21
            Annotation Date                   :: 10/21/2024 21:26:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,296
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 4,144
            CDSs (with protein)               :: 4,144
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 23 of 90
            Pseudo Genes (incomplete)         :: 74 of 90
            Pseudo Genes (internal stop)      :: 5 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:21:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2851
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AZXF01000050.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
FEATURES             Location/Qualifiers
     CDS             complement(<1..2847)
                     /NRPS_PKS="Domain: Condensation_LCL (2-157). E-value:
                     4.7e-57. Score: 185.2. Matches aSDomain:
                     nrpspksdomains_B042_RS0116820_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (326-721). E-value: 1.8e-97.
                     Score: 318.4. Matches aSDomain:
                     nrpspksdomains_B042_RS0116820_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (828-896). E-value: 1.6e-29. Score:
                     93.9. Matches aSDomain:
                     nrpspksdomains_B042_RS0116820_PCP.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /locus_tag="B042_RS0116820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_028649299.1"
                     /sec_met_domain="Condensation (E-value: 1.4e-32, bitscore:
                     99.0, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.8e-112, bitscore:
                     361.3, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.5e-20, bitscore:
                     59.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGRAYRDRSRGAVPDWEPLEIQYADYALWQEDVLGEDDDPESLA
                     AEQIAYWRKALEGVPEALDLPADRPRPAVASYRGGVAPIALSARTHRALLRIAAEHEAT
                     LFMVVQAALAALLTRTGAGSDIPLGTPVAGRDDEGLEDLVGFFVNTLVLRTDTSGDPAF
                     DELLARVKEADLAAFAHQDLPFDRLVEVVNPARSTAHHPLFQVMLVLQNNARGDIGLPG
                     VEVSEEPFRTGRAKFDLTFIVSEDHSPDGSPAGVTGGLEYATDLFDHETAVALAARLSR
                     VLDAVAADPGTRIGTIGLLSPEERRTLEAWNATDRQIPFDTVPAAFEDHVRRTPGAVAV
                     VFGNEDLTYAELDTRANRLAHHLVASGAERGDVLAVLIERGIDLAVTVLAAAKAGTPYL
                     LLDPDFPDDRLRSLAEGAGARRLVTRAEWADRLTTGTAPIRLDAEADAIARRPGHAPGV
                     ELGPEDGVCVMFTSGSTGRPKGVLTPHRAVVGTFLGQDFVDLGPDQVWLQCALLSWDVF
                     VLEFWGALLNGAHCVLQPGQTPEPELIESLAAEHGVTVLWLSAGLFNLMLDEYPGVFSG
                     VRQVMTGGEAPSPEHLRRFRERFPHVRLVHGYGPVEAVIFTNAATVTDVPGGGVPVGRP
                     LANKRTYVLDERLRPVPVGVVGEVYAAGIGLANGYLGQPGATAERFVADPFGGPGERMY
                     RTGDLGRWTTGGELVVVGRADGQVKIRGFRIEPGEIEAHLAAVPGVSQCAVVVREDRPG
                     DKRLVGYAVPEAGAGLAPVDLRSSLARSLPQYMVPAAVVVLEALPLTGNGKLDRRALPE
                     PDFGELSQGRAPRDEREEELCALFAEVLGLESVTIDDSFFDLGGHSLLATRLLSRIRGA
                     LGVELTVRELFHAPTVAGVAERIGSGNAVPARPALTVQDRPERIPLSYAQRRLWFLDRV
                     EGPSATYNIPVVLRLRGAL"
     source          1..2851
                     /db_xref="taxon:1137268"
                     /isolation_source="not provided; submitted under MIGS 2.1"
                     /mol_type="genomic DNA"
                     /organism="Nocardiopsis sp. CNT312"
                     /strain="CNT312"
                     /submitter_seqid="B042DRAFT_scaffold_44.45_C"
     gene            complement(<1..>2851)
                     /locus_tag="B042_RS0116820"
     region          1..2851
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2851
                     /SMILES="NC(C(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2851
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:2847](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2847)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(62..94)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B042_RS0116820_0007"
                     /evalue="3.40E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="B042_RS0116820"
                     /protein_end="928"
                     /protein_start="917"
                     /score="10.0"
                     /tool="antismash"
                     /translation="SYAQRRLWFLD"
     aSDomain        complement(158..361)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B042_RS0116820_PCP.1"
                     /evalue="1.60E-29"
                     /label="B042_RS0116820_PCP.1"
                     /locus_tag="B042_RS0116820"
                     /protein_end="896"
                     /protein_start="828"
                     /score="93.9"
                     /tool="antismash"
                     /translation="EEELCALFAEVLGLESVTIDDSFFDLGGHSLLATRLLSRIRGALG
                     VELTVRELFHAPTVAGVAERIGS"
     aSModule        complement(158..2839)
                     /complete
                     /domains="nrpspksdomains_B042_RS0116820_Condensation_LCL.1"
                     /domains="nrpspksdomains_B042_RS0116820_AMP-binding.1"
                     /domains="nrpspksdomains_B042_RS0116820_PCP.1"
                     /locus_tags="B042_RS0116820"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(653..715)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B042_RS0116820_0006"
                     /evalue="8.50E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="B042_RS0116820"
                     /protein_end="731"
                     /protein_start="710"
                     /score="37.0"
                     /tool="antismash"
                     /translation="VGRADGQVKIRGFRIEPGEIE"
     aSDomain        complement(683..1867)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B042_RS0116820_AMP-binding.1"
                     /evalue="1.80E-97"
                     /label="B042_RS0116820_AMP-binding.1"
                     /locus_tag="B042_RS0116820"
                     /protein_end="721"
                     /protein_start="326"
                     /score="318.4"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEDHVRRTPGAVAVVFGNEDLTYAELDTRANRLAHHLVASGAERG
                     DVLAVLIERGIDLAVTVLAAAKAGTPYLLLDPDFPDDRLRSLAEGAGARRLVTRAEWAD
                     RLTTGTAPIRLDAEADAIARRPGHAPGVELGPEDGVCVMFTSGSTGRPKGVLTPHRAVV
                     GTFLGQDFVDLGPDQVWLQCALLSWDVFVLEFWGALLNGAHCVLQPGQTPEPELIESLA
                     AEHGVTVLWLSAGLFNLMLDEYPGVFSGVRQVMTGGEAPSPEHLRRFRERFPHVRLVHG
                     YGPVEAVIFTNAATVTDVPGGGVPVGRPLANKRTYVLDERLRPVPVGVVGEVYAAGIGL
                     ANGYLGQPGATAERFVADPFGGPGERMYRTGDLGRWTTGGELVVVGRADGQVKIR"
     CDS_motif       complement(803..892)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B042_RS0116820_0005"
                     /evalue="7.60E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="B042_RS0116820"
                     /protein_end="681"
                     /protein_start="651"
                     /score="43.8"
                     /tool="antismash"
                     /translation="PVGVVGEVYAAGIGLANGYLGQPGATAERF"
     CDS_motif       complement(1391..1447)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B042_RS0116820_0004"
                     /evalue="1.90E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="B042_RS0116820"
                     /protein_end="485"
                     /protein_start="466"
                     /score="26.5"
                     /tool="antismash"
                     /translation="CVMFTSGSTGRPKGVLTPH"
     CDS_motif       complement(2216..2332)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B042_RS0116820_0003"
                     /evalue="1.50E-21"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="B042_RS0116820"
                     /protein_end="210"
                     /protein_start="171"
                     /score="68.9"
                     /tool="antismash"
                     /translation="EADLAAFAHQDLPFDRLVEVVNPARSTAHHPLFQVMLVL"
     aSDomain        complement(2375..2839)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B042_RS0116820_Condensation_LCL.
                     1"
                     /domain_subtypes="Condensation_LCL"
                     /evalue="4.70E-57"
                     /label="B042_RS0116820_Condensation_LCL.1"
                     /locus_tag="B042_RS0116820"
                     /protein_end="157"
                     /protein_start="2"
                     /score="185.2"
                     /specificity="C activity: inactive"
                     /specificity="Active site motif: -------"
                     /tool="antismash"
                     /translation="GRAYRDRSRGAVPDWEPLEIQYADYALWQEDVLGEDDDPESLAAE
                     QIAYWRKALEGVPEALDLPADRPRPAVASYRGGVAPIALSARTHRALLRIAAEHEATLF
                     MVVQAALAALLTRTGAGSDIPLGTPVAGRDDEGLEDLVGFFVNTLVLRTDT"
     CDS_motif       complement(2384..2473)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B042_RS0116820_0002"
                     /evalue="5.00E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="B042_RS0116820"
                     /protein_end="154"
                     /protein_start="124"
                     /score="51.0"
                     /tool="antismash"
                     /translation="DIPLGTPVAGRDDEGLEDLVGFFVNTLVLR"
     CDS_motif       complement(2744..2779)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B042_RS0116820_0001"
                     /evalue="3.40E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="B042_RS0116820"
                     /protein_end="34"
                     /protein_start="22"
                     /score="16.4"
                     /tool="antismash"
                     /translation="QYADYALWQEDV"
ORIGIN
        1 tccagggcac cgcgcaggcg cagcaccacc gggatgttgt aggtggcgct cggcccctcc
       61 acacggtcca ggaaccagag tctgcgttga gcgtaggaga gggggatccg ttcggggcgg
      121 tcctggacgg tgagcgcggg ccgggccggg acggcgttcc cggagccgat gcgttcggcg
      181 acaccggcga cggtcggggc atggaacagc tcccggacgg tcagctcgac cccgagcgcg
      241 ccccggatgc ggctgagcag gcgggtggcg agcagggagt gcccgcccag gtcgaagaag
      301 gagtcgtcga tcgtcaccga ctccaaaccc agcacctccg cgaacagagc gcacagctct
      361 tcctcccgtt catcgcgagg agcccggccc tgggacagtt cgccgaagtc gggctcgggc
      421 agcgcacggc ggtccagctt gccgttgccg gtgaggggga gggcctccag caccaccacc
      481 gcggcgggca ccatgtactg gggcagggag cgtgcgagcg acgagcgcag gtccacgggg
      541 gcgagaccgg ccccggcttc gggcaccgcg tagcccacca ggcgcttgtc accgggccgg
      601 tcctcgcgga ccaccaccgc gcactgggac accccgggca ccgccgccag gtgcgcctcg
      661 atctctccgg gttcgatgcg gaaaccgcgg atcttgacct ggccgtcggc ccggccgacc
      721 accaccagct cacctccggt ggtccaccgg cccaggtcgc cggtccggta catccgctcc
      781 cccgggccgc cgaaggggtc ggccacgaag cgctcggcgg tggcaccggg ctggcccagg
      841 tagccgttgg ccaggccgat ccccgccgcg tacacctcgc ccaccacgcc gacgggcacc
      901 gggcgcagcc gttcgtccaa gacataggtg cgcttgttgg ccagggggcg gccgaccggc
      961 accccgcccc cgggcacgtc ggtgaccgtg gcggcgttgg tgaagatcac cgcctcgacg
     1021 gggccgtagc cgtgcaccag ccgcacgtgc gggaaccgct cccggaaccg ccgcaggtgc
     1081 tcgggggagg gcgcctcacc gccggtcatc acctgccgca cccccgagaa cacccccggg
     1141 tactcgtcca gcatgaggtt gaacagcccc gccgacaacc acaggaccgt gaccccgtgt
     1201 tccgcggcca gggactcgat gagttcgggc tcgggggtct gccccggctg gaggacgcag
     1261 tgggcaccgt tgagcagcgc gccccagaac tccagcacga acacgtccca ggagagcagt
     1321 gcgcactgga gccacacctg gtccggcccc aggtccacga agtcctggcc caggaacgtg
     1381 cccaccaccg cgcggtgcgg tgtgagcacg cccttggggc ggccggtgga ccccgacgtg
     1441 aacatcacgc acactccgtc ttcggggccg agttccacac cgggggcgtg gccggggcgg
     1501 cgggcgatcg cgtcggcctc ggcgtcgaga cggatcggcg cggtcccggt ggtgaggcgg
     1561 tcggcccact cggcccgggt gaccaggcga cgtgcgcccg ccccctcggc caggctccgc
     1621 aggcggtcgt cggggaagtc ggggtcgagc aggaggtagg gggttccggc cttggccgcc
     1681 gccaggaccg tgaccgcaag gtcgatcccg cgttcgatga gaacggcgag cacgtcgccg
     1741 cgttcggctc cgctggcgac caggtggtgg gcgagccggt tggcgcgcgt gtccagttcg
     1801 gcgtaggtga ggtcctcgtt gccgaacacg accgccaccg cgccgggggt gcgccgcacg
     1861 tggtcctcga acgccgcggg gacggtgtcg aacgggatct ggcggtcggt ggcgttccat
     1921 gcttccaggg tccgccgctc ctccggcgag aggaggccga tggtgccgat acgcgtaccg
     1981 gggtcggcgg cgaccgcgtc caggacccgg gacaggcgtg ccgccagggc aacggcggtc
     2041 tcgtggtcga acaggtcggt ggcgtactcc aggcctcccg tgacaccggc cggggagccg
     2101 tccggcgagt ggtcctcgct cacgatgaac gtgaggtcga acttggccct accggtgcgg
     2161 aagggctcct ccgacacctc gacgcccgga agcccgatgt cgccgcgggc gttgttctgg
     2221 agcaccagca tgacctggaa gagcgggtgg tgcgcggtgg agcgggcggg gttgaccacc
     2281 tccacgaggc ggtcgaaggg caggtcctgg tgggcgaagg cggccaggtc ggcctccttc
     2341 acccgggcca gcagctcgtc gaacgccggg tcgccggagg tgtcggtgcg caggaccagg
     2401 gtgttgacga agaaccccac caggtcctcc agcccttcgt cgtcccggcc cgccacggga
     2461 gtgccgagcg gaatgtcgga accagcaccc gtccgggtca gcagtgccgc cagcgccgcc
     2521 tgtacgacca tgaacagggt cgcctcgtgc tctgccgcga tccgcaggag cgcacggtgg
     2581 gtccgcgcgg agagggcgat gggcgcgaca ccgccccggt aggaggcgac ggcgggccgg
     2641 ggccggtcgg cgggcaggtc cagcgcctcg gggacgccct ccagggcctt gcgccagtac
     2701 gcgatctgct cggcggccag gctctcgggg tcgtcgtcct caccgaggac gtcctcctgc
     2761 cagagcgcgt agtcggcgta ctggatctcc agcggctccc agtccggaac cgcgccccgg
     2821 gaccggtccc ggtaggcccg ccccagatcc c
//
