LOCUS       NZ_AZXF01000030        10417 bp    DNA     linear   CON 21-OCT-2024
DEFINITION  Nocardiopsis sp. CNT312 B042DRAFT_scaffold_24.25_C, whole genome
            shotgun sequence.
ACCESSION   NZ_AZXF01000030
VERSION     NZ_AZXF01000030.1
KEYWORDS    WGS; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Nocardiopsis sp. CNT312
  ORGANISM  Nocardiopsis sp. CNT312
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsidaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 62921)
  AUTHORS   Jensen,P., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            assembly :: Velvet v. 1.1.04; ALLPATHS v. R41043
            investigation_type                :: bacteria_archaea
            project_name                      :: Nocardiopsis sp. CNT312
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi14643
            Funding Program                   :: DOE-CSP-ICBG 2011
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. R41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000515115.1-RS_2024_10_21
            Annotation Date                   :: 10/21/2024 21:26:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,296
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 4,144
            CDSs (with protein)               :: 4,144
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 23 of 90
            Pseudo Genes (incomplete)         :: 74 of 90
            Pseudo Genes (internal stop)      :: 5 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:21:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 34590
            Orig. end                         :: 45007
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AZXF01000030.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
FEATURES             Location/Qualifiers
     region          1..10417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5417](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5417
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(280..294)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.0"
                     /tool="antismash"
     gene            complement(508..1179)
                     /locus_tag="B042_RS26145"
     CDS             complement(508..1179)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598649.1"
                     /locus_tag="B042_RS26145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="copper resistance CopC family protein"
                     /protein_id="WP_051415702.1"
                     /transl_table=11
                     /translation="MSSETTRTPLRRPTATATAAAAVLALAMAPSPALAHDALIDSSPG
                     DGATLDAVPEEVVLTFNNEPLANGSASTVVVTGPDERTYTGEAPLDFDGTDVTAELLPL
                     DQAGDYTVAFRIVSSDGHPIQDTLEFTLTEEAVTTAAEEVPAETDGQETAVAEETSEEA
                     APETPAVEADEQAVSGAEEGTGGKTLLLVLGAVLVLFLVLSMIVLVRARLRGIEAAKGD
                     E"
     gene            complement(1208..1786)
                     /locus_tag="B042_RS26150"
     CDS             complement(1208..1786)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911702.1"
                     /locus_tag="B042_RS26150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_028648898.1"
                     /transl_table=11
                     /translation="MRPAHGTLRLTRTALLAGACTWLSWTGHNLSSATPAPAAGLVLAT
                     VLLVPVLWHFTKVMRGFTDIFAVLACAQLGLHAVLQGTGGPTPSFGAEHSGHGLLAHLV
                     ELGLAPGMLLAHLWAALLAAALLAHGESALWRLTALLSRALPPLLLVRTPPGPATTAPP
                     APPAAVPTASAAPSAHGPRAPPGSPRVPA"
     gene            complement(1924..2190)
                     /locus_tag="B042_RS0114450"
     CDS             complement(1924..2190)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017616169.1"
                     /locus_tag="B042_RS0114450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="WhiB family transcriptional regulator"
                     /protein_id="WP_028648899.1"
                     /transl_table=11
                     /translation="MNRLYHESEDWVVQGRCRSYDPELFFPVSSTGIGRADTEQALTVC
                     RSCPVRQECLRWALRVGEAHGVWGGTTPEERRYMRRAVLAPAS"
     gene            2995..3483
                     /gene="ectA"
                     /locus_tag="B042_RS0114455"
     CDS             2995..3483
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546867.1"
                     /locus_tag="B042_RS0114455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_255342348.1"
                     /transl_table=11
                     /translation="MEDGPRMWRLAGQAGMDVNSPYAYTLWCRDFAKTSVVARDPDGGL
                     AAYVTGHVRPDSPDTYFLWQVAVDSAYRGQRLARRMLDFIGDRIADQGIRYLEATVTPD
                     NTASRALFSSFARDRDTEIAWSPLFTEEHFPQDGGTPHEPEDLVRIGPFGPRTSQERA"
     gene            3573..4838
                     /gene="ectB"
                     /locus_tag="B042_RS0114460"
     CDS             3573..4838
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 4.5e-74.
                     Score: 241.4. Matches aSDomain:
                     nrpspksdomains_B042_RS0114460_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610153.1"
                     /locus_tag="B042_RS0114460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_028648901.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7.3e-77, bitscore:
                     250.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFQRLESEVRGYCRNWPVVFDKAVGSHVYDESGKPYLDFFAGA
                     GSLNYGHNNPELKASLIEYLTDDRIVHSLDAYSTAKREFLKTFEEVLLKPRGLDYKIQF
                     PGPAGNNAVEAALKLARKYTGRQTIINFTNGFHGMTLGALAVTGNSMKRGGAGVPLDHV
                     ATMPFDNYLDGKTPDFLWLRTLLEDSGSGLDKPAAVIVETVQGEGGINAASAEWLRGLS
                     ELCTEYGILMIVDDIQMGCGRTGDFFSFEEAGITPDIVTLSKSISGYGLPMALTLFKRE
                     LDVWSPGEHNGTFRGFNPAMVTGVEAMHRYWSDDSFARATKAKGEMVAARLQEIADEVP
                     ETGAHVRGRGLARGIAFEEAAVAKRVTAEAFDRGLLLETSGPEDEVAKLLPPLTTTEDE
                     FTQGLDIIAESVRAAVKVAQPA"
     aSDomain        3636..4646
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B042_RS0114460_Aminotran_3.1"
                     /evalue="4.50E-74"
                     /label="B042_RS0114460_Aminotran_3.1"
                     /locus_tag="B042_RS0114460"
                     /protein_end="358"
                     /protein_start="21"
                     /score="241.4"
                     /tool="antismash"
                     /translation="FDKAVGSHVYDESGKPYLDFFAGAGSLNYGHNNPELKASLIEYLT
                     DDRIVHSLDAYSTAKREFLKTFEEVLLKPRGLDYKIQFPGPAGNNAVEAALKLARKYTG
                     RQTIINFTNGFHGMTLGALAVTGNSMKRGGAGVPLDHVATMPFDNYLDGKTPDFLWLRT
                     LLEDSGSGLDKPAAVIVETVQGEGGINAASAEWLRGLSELCTEYGILMIVDDIQMGCGR
                     TGDFFSFEEAGITPDIVTLSKSISGYGLPMALTLFKRELDVWSPGEHNGTFRGFNPAMV
                     TGVEAMHRYWSDDSFARATKAKGEMVAARLQEIADEVPETGAHVRGRGLARGIAFE"
     misc_feature    complement(4901..4915)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     misc_feature    4929..4943
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     gene            5001..5417
                     /locus_tag="B042_RS0114465"
     CDS             5001..5417
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573110.1"
                     /locus_tag="B042_RS0114465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_028648902.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.6e-55, bitscore:
                     178.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLDEVNGTDRDVEHGNWRSRRVILAKEGVGFSFHETVLYAG
                     TETSMWYANHIELVHCIEGEAELTNDETGEKHVITPGTLYLLNGHEKHTVRPKTDFRVL
                     CVFNPPVTGREVHDENGVYPLIVEEPAEGDAPVA"
     gene            5882..6739
                     /gene="ehuB"
                     /locus_tag="B042_RS0114470"
     CDS             5882..6739
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567885.1"
                     /locus_tag="B042_RS0114470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_231494098.1"
                     /transl_table=11
                     /translation="MGLAVAGLAACSRVDGGGDGGGEGGGQSTLDRLREQGFVTVGLAS
                     EIPFGFIDDAGNPAGQSPAVAQAVFEELGVPEIQAESVAWDGLIPGLQASRFDVIAAGM
                     FINPERCQQVAFSEPTATSPEAFLVNEGNPDDITHFTSFVDNSDLKLAVLNGSVEQGYA
                     EHFGIPEDQLELIPDQTTGYELLDAGRVDAVSMLSVSHTYLLLDRGGPFEVTEAFFPEI
                     DGTQEKGWGGLAFRQEEASLVEEVNRVIAEFKENGRLLEIGEEWGFTEADLPDDVTTAE
                     LCEG"
     gene            6746..7474
                     /gene="ehuC"
                     /locus_tag="B042_RS0114475"
     CDS             6746..7474
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156203.1"
                     /locus_tag="B042_RS0114475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_028648904.1"
                     /transl_table=11
                     /translation="MQFLIERLPLYLDGAWVTIQLTIGGCALAFVLAMAFGILGSMKHG
                     LPRAVATVYVEVFRGIAALVLMFWMAYALPLATGFQLNDVFAAILAVGLNVGAYGAEVV
                     RAAINAVPRAQIEGTVALNMSWFQRTRLVVVPQAWAQMLPTFGNLVIELMKATAVAYLI
                     GVTDLTAVADQMREATGETIVAFSGALILYYVIAQVLIFGMRLLERRANRKLGRTPPGG
                     AGLFALLRPPKTGTKVGVAQ"
     gene            7504..8109
                     /gene="ehuD"
                     /locus_tag="B042_RS0114480"
     CDS             7504..8109
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573113.1"
                     /locus_tag="B042_RS0114480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_369797708.1"
                     /transl_table=11
                     /translation="MPTLLEGLLVTVQVTAMGYLIALSLGLLIAIARRVPVAGSVLHVV
                     MEFVRTTPLVVQLVFVFFLTPPNISGMTVGVVVIGVHYSAYTAEVYRSGIEGVAKGQWE
                     AARALSLPATRTWGAIVLPQAIRKVIPALGNYLISMFKDTPLLIAIGVAELLTRAQQIG
                     SASFRVVEAYTMVGFLFLLVSIPSAIIIRRLERRYAAV"
     gene            8096..8920
                     /gene="ehuA"
                     /locus_tag="B042_RS0114485"
     CDS             8096..8920
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017590341.1"
                     /locus_tag="B042_RS0114485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_028648906.1"
                     /transl_table=11
                     /translation="MPPSDAPDTEEPQAGEGQPQIVFDKVVKRFGDLTVLDHLDFSVEP
                     GERVTLIGPSGSGKTTILRLLMTLEKITEGHIQVAGEPFSHMRRGSKLVPASEGYLREK
                     RKSIGMVFQQFNLFPNMSVRKNVTEAPVHVLGLPKDEANARAEELLDLVGLGEKIDAHP
                     TQLSGGQQQRVAIARALAMRPDILLLDEVTSALDPELVAGVLDVLRQVAETTDITMLCV
                     THEMAFARDVSHRVLMFDKGRIAEEGTPEKIFEDPEEERTKAFLSSVLDNGH"
     gene            9078..10013
                     /locus_tag="B042_RS0114490"
     CDS             9078..10013
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017567889.1"
                     /locus_tag="B042_RS0114490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_028648907.1"
                     /sec_met_domain="Abi (E-value: 2.4e-10, bitscore: 32.7,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTETRSWGLTSAGVFFAVVVFAMLASASLRAEILGPDLPIFLTGW
                     GAEVVGAVMAFVLVRWFGDRDGLDLRIRLALEEHPVGRELGLLLGFLGGFIVLMVLLSG
                     IFSLLSGEIGADLRMSAAMVSRLLFMLALPLLFMDRAGYTVSGPGSAMALLVVIVREPW
                     RWLGLIPVVFFLVVLGVAAMPAYGLTGLSLAALGLVLAFALISVCEEIFYRGMVQTRLE
                     LVMGRWGGIFATAGLYSLSYALTQPYDIVGQFSRTDPVYDVGLALCIYGSVALFHGCLW
                     TCYRNIWLNVLVHLCGLLILLRPEVTSPLF"
ORIGIN
        1 ccggtatggg gaccacggtc gccggtgtcc tgctcaacag cgacggcaac ttctggttca
       61 acgtcggaga ctcgcggacc taccgcctgg agcggggccg cctgcgccag ctctccgagg
      121 acgactcccc ggcgctgccg ccctccgagg acggccgacc ggtcaccacg aacttcatca
      181 cccagtccct gggcggcagc gcgggcggcg tgatggtccc ccacgtggga cgcgacgacg
      241 actccgaccc cagcgcctgg ctcatgtgca gcgacggcct gtccgacctg gtcctgcccg
      301 aggagatgga gcggatgatc ggcgaggccg gcagcgacga ggcggccgtg cacgccctct
      361 ggcaggccgc gatggaggcc gggggcaagg acaacatcag cattctgctc gcacgtcggc
      421 tgtgagccct ccggggcccg tggacagagc acaggggtgg tgcgtcgacc gaggccgacg
      481 caccacccct gtcaccgttc gccgccgtta ctcgtcgccc ttcgcggctt cgatcccgcg
      541 caggcgtgca cgcacgagaa cgatcatgga gaggacgagg aacaggacga ggacggcgcc
      601 caggacgagg agcagcgttt tgccgcccgt cccctcttcc gccccgctca cggcctgctc
      661 gtcggcttcg accgcgggcg tctccggggc ggcctcctcg ctggtttcct cagcgacggc
      721 cgtctcctgc ccgtcggtct cggccggcac ctcctccgcg gcggtcgtga cggcctcctc
      781 ggtcagtgtg aactccagtg tgtcctggat ggggtggccg tccgaggaga cgatgcggaa
      841 ggcgacggtg tagtcgcccg cctggtccag gggaagcagt tccgccgtga cgtcggtgcc
      901 gtcgaagtcg aggggcgcct caccggtgta ggtccgctcg tccggaccgg tgacgacgac
      961 cgtgctcgcg ctgccgttcg ccagcggttc gttgttgaag gtgaggacga cctcctcggg
     1021 gaccgcgtcc agggtcgccc cgtccccggg cgaggagtcg atgagtgcgt cgtgggccag
     1081 ggcgggggag ggggccatgg ccagcgccag gacggccgct gcggccgtcg ccgtcgcggt
     1141 ggggcggcgc agtggtgtgc gggtggtctc ggatgacatg cgggcttctt tccgttccgg
     1201 ggtgttctca ggctgggacg cgcggagaac ccgggggcgc gcgcgggccg tgcgcggacg
     1261 gcgcggcgga cgcggtggga acggcggccg ggggcgccgg aggcgcggtc gtcgcagggc
     1321 cgggaggtgt gcgtacgagc agcagcggtg gcagggcccg ggacagcagg gccgtgagcc
     1381 gccacagggc ggactcgccg tgggcgagca gagcggctgc gagcagcgcc gcccacaggt
     1441 gggctaggag catgcccggg gccaggccca gctcgacgag gtgcgcgagg aggccgtgcc
     1501 cgctgtgttc cgccccgaag gagggcgtcg gcccgccggt gccctggagg acggcgtgga
     1561 ggccgagctg ggcgcaggcc aggaccgcga agatgtcggt gaagccgcgc atcaccttgg
     1621 tgaagtgcca cagcaccgga acgaggagca cggtggccag gacgagaccg gccgcgggtg
     1681 cgggggtggc ggacgacagg ttgtgccccg tccaggacag ccatgtgcac gcaccggcca
     1741 ggagtgcggt gcgggtgagt cggagggtgc cgtgggcggg gcgcacgtga ccgaacctag
     1801 gacctggcag gtggggccgc aaggaggggg ctcacacccg gctgacggtg agaagcgcgt
     1861 gagccgcccg gcgcgggccg tcgggtgcgg tccccctcgc gcccgacggc ccgtgccgac
     1921 gccttacgag gcgggggcca ggacggcgcg ccgcatgtag cggcgctcct ccggggtggt
     1981 ccccccccag acgccgtggg cctcgccgac tcgcagcgcc caccgcaggc actcttggcg
     2041 aacagggcaa ctgcggcaga cggtgagagc ctgctcggtg tcggcgcgtc cgattccggt
     2101 gctgctgacc gggaagaaga gctccgggtc gtaggagcgg cagcgtccct ggacgaccca
     2161 gtcctcgctc tcgtggtaca ggcggttcac gtgagcctcc gatccgtggc tgagatccgt
     2221 ggtcggtgtc ccccgccgtg ggtcccacgg cgggaggggc ctcggcctca ccacggtttc
     2281 cgggcgcctc gctcccgggt ctggggcgaa gcgatgcgac caagtgtagt acgacctctt
     2341 gtagtactac atggagtctg ctggaaaagc acagatccat caaggccccg aggagcgcgg
     2401 gcggggcgga gggcgtcttc ccggatgccg tcgccgtgcg gggtctgcgc cgagggggtg
     2461 cgggcttcgg ttcggaagtg cctgcccaga agcggttccg tgcgcttcgg tgcggtgtct
     2521 ttgcgatgtg tctcacgcgc gtgaagcgtt atgtcgcgag ctttccaccg aattgatgtg
     2581 acttaatgca ctgttaataa tgcccctgaa ggctctatgt ttgaccctga gcgcgatccg
     2641 ccgcacgcgc cgaaccgcgc ggcgacccac cgtggtcggc ccgcgcgcga aaggcgaaat
     2701 cccctgatgg acagggcact cacggtggga gccgcacacc gaaggccccc cgcgagggct
     2761 ctggccaggt cgttgttgcg ctgctaaata aacgggtcct acccgctgct gatctggtca
     2821 aacatgtggg cggaatctgc ctgaatcatt cttggtgttc ggttgctaag ttcattcgac
     2881 gtgccacatc tacagacgaa cctccaagca cgcgaccgga ccgacggcgg ggccaacggc
     2941 tctggggcca caggggccgc gggccgcggg gacgtgcgcc tggcgcagcc gtccctggag
     3001 gacgggccgc gcatgtggcg gctggcgggc caggcgggga tggacgtcaa ctctccgtac
     3061 gcctacacgc tctggtgcag ggacttcgcg aagacgagcg ttgtcgcccg cgatcccgac
     3121 ggcggtctcg ccgcctacgt caccggacac gtgcgccccg acagcccgga cacgtacttt
     3181 ctctggcagg tcgccgtcga ctccgcgtac cgcggacagc ggctggcccg ccggatgctc
     3241 gacttcatcg gtgaccggat cgccgaccag ggaattcgtt atctcgaagc gaccgttaca
     3301 cccgacaaca ccgcatcacg tgcgctgttc tcctcgttcg cccgggaccg cgacaccgag
     3361 atcgcatgga gcccgctctt caccgaggag cacttcccgc aggacggcgg gaccccccac
     3421 gaaccagagg acctcgtccg catcggcccc ttcgggccgc ggacgagcca agagcgtgcc
     3481 tgaccatccg caacggatcg accgatcctg acccaccccg tcgtcaaagc cacgagccca
     3541 cgaacttggg gaaaggaacc aaggaacaca cgatggagac cttccagcgc ctcgagtccg
     3601 aagtccgcgg atactgccgg aactggcctg tcgtcttcga caaggccgtc ggaagccacg
     3661 tctacgacga gagcggcaag ccctacctgg acttcttcgc cggggccgga tccctcaact
     3721 acgggcacaa caaccccgag ttgaaggcct cgctcatcga gtacctcacc gacgatcgga
     3781 tcgtccacag cctcgacgcc tacagcaccg ccaaacgcga gttcctgaaa acgttcgagg
     3841 aggtcctcct caaacccagg ggcttggact acaagatcca gttccccggc ccggcgggca
     3901 acaacgccgt cgaggccgcg ctgaagctgg cccgcaagta caccggacgc cagaccatca
     3961 tcaacttcac caacggcttc cacggcatga ccctgggcgc gctggccgtg accggcaact
     4021 ccatgaagcg cggcggtgcc ggtgtcccgc tggaccacgt cgccaccatg cccttcgaca
     4081 actacctcga cggcaagacc ccggacttcc tctggttgcg caccctgctg gaggacagcg
     4141 gcagtggcct ggacaagccg gccgcggtca tcgtcgagac ggtccagggc gagggcggca
     4201 tcaacgccgc cagcgccgag tggctgcgcg ggctctccga gctgtgcacc gagtacggca
     4261 tcctcatgat cgtcgacgac atccagatgg gctgcggccg caccggcgac ttcttcagct
     4321 tcgaggaggc cgggatcacc ccggacatcg tcacgctctc caagtccatc agcggctacg
     4381 gtctgccgat ggccctcacg ctgttcaagc gcgagctgga cgtgtggagc ccgggcgagc
     4441 acaacggcac gttccgcggc ttcaacccgg ccatggtgac cggcgtcgag gcgatgcacc
     4501 gctactggag cgacgactcc ttcgcccggg ccaccaaggc caagggcgag atggtggccg
     4561 cgcgtctcca ggagatcgcc gacgaggtcc ccgagaccgg tgcgcacgtg cggggtcggg
     4621 gcctggcccg gggtatcgcc ttcgaggagg ccgccgtcgc caagcgggtc accgccgagg
     4681 ccttcgaccg cggcctgctc ctggagacct ctggccccga ggacgaggtg gccaagctcc
     4741 tgccgcccct caccacgacc gaggacgagt tcacccaggg cctcgacatc atcgccgagt
     4801 ccgtccgtgc cgcggtgaag gtggcgcagc cggcctgaga caggccctcc cggggtcctg
     4861 tgggccccgg ggctcccccc gaaggaaaca cagggttcac ggggccggtc ggcaccctcc
     4921 cggaagggaa gccgaccggc cctgtccgtg ttgaatggac cgaagcgatt ggcgcacgag
     4981 cgcgttaaag gagaccatca gtgatcgttc gcagcctgga cgaggtcaac ggcaccgacc
     5041 gcgacgtcga gcacggcaac tggcgcagcc gccgggtcat cctggccaag gagggggtgg
     5101 gcttctcctt ccacgagacc gtgctgtacg cgggtaccga gaccagcatg tggtacgcca
     5161 accacatcga actggtgcac tgcatcgagg gtgaggccga actcaccaac gatgagaccg
     5221 gggagaagca cgtcatcacc ccgggcaccc tctacctgct caacggtcac gagaagcaca
     5281 cggtccgccc caagacggac ttccgtgtgc tgtgcgtgtt caacccgccg gtgaccgggc
     5341 gcgaggtgca cgacgagaac ggcgtgtacc cgctcatcgt cgaggagccg gccgagggcg
     5401 acgccccggt cgcctgacga ccgggcggca tgcgtgcggg cgggaccctg ggggtcccgc
     5461 ccgtatccgt ttcacgaggc ccaaccccca cagtgacccc ctgagccacc cacggtcacg
     5521 cggtacaggt ggcggcgcac ggcggtgttc tctcggcgtt cactggctgg tagaggcccc
     5581 ataaccagtg cataacggac taatcgtgcc tagttactga taagcggcct gggtagggag
     5641 aatcccgtac cagtgccgcc ggcccagtcc tgtcggcggc cggtctccgt gccgtgtgcc
     5701 accccctcgc ggagacctca gaggcaggag ttcctgtccc ccgaacgcgc ggcgtcgccg
     5761 tctgcttgac gaggcggcgc cgctcccccg agttctacga ggagcagtcg tgaagaagca
     5821 ggtaccagcg gtcaaccagg gtgcgacgtg gggccgccga gacgcgttcc gactgggcgg
     5881 tgtggggctc gccgtggccg gtctcgccgc ttgcagccgg gtggacggtg gcggtgacgg
     5941 tggcggtgag ggtggcggcc agtccactct ggaccgcctg cgcgagcagg gcttcgtcac
     6001 cgtgggcctc gccagcgaga tccccttcgg cttcatcgac gacgccggca acccggcggg
     6061 tcagtccccg gccgtggccc aggcggtctt cgaggagctc ggcgttcccg agatccaggc
     6121 cgaatccgtc gcctgggacg gtctgatccc gggcttgcag gccagccggt tcgacgtcat
     6181 cgccgccggc atgttcatca accccgaacg ctgccagcag gtcgccttca gcgagccgac
     6241 cgccacctcc ccggaggcct tcctcgtcaa cgagggcaac ccggacgaca ttacccactt
     6301 cacgtcgttc gtggacaact ccgacctcaa gctggcggtc ctcaacggct ccgtggaaca
     6361 gggctacgcc gagcacttcg gtatccccga ggaccagctc gaactgatcc cggaccagac
     6421 caccggctat gagctgctgg acgcgggccg tgtggacgcc gtctccatgc tgagcgtgtc
     6481 ccacacctat ctgctgctcg accgcggcgg gcccttcgag gtcaccgagg ccttcttccc
     6541 cgagatcgac ggcacgcagg agaagggctg gggcggcctg gccttccgcc aggaagaggc
     6601 ctcgctcgtg gaggaggtca accgggtcat cgccgagttc aaggagaacg gacgcctcct
     6661 ggagattggc gaggagtggg gcttcaccga ggctgacctg cccgacgacg tcacgaccgc
     6721 cgaactctgc gaaggctgat accgcgtgca gttcttgatc gagcggctgc cgctctatct
     6781 cgacggcgcg tgggtcacca tccagctcac catcggcggc tgtgccctgg ccttcgtcct
     6841 ggccatggcc ttcggcatcc tgggcagcat gaagcacggg ctgcccaggg ccgtggccac
     6901 ggtctacgtg gaggtcttcc ggggcatcgc cgccctggtg ctgatgttct ggatggccta
     6961 cgcgcttccg ctggccaccg ggttccagct caacgatgtg ttcgccgcga tcctggcggt
     7021 cgggctgaac gtcggcgcct acggtgccga ggtggtccgt gccgcgatca acgctgtgcc
     7081 cagggcccag atcgagggca cggtcgcgct caacatgtcc tggttccagc gcacccggct
     7141 ggtcgtggtt ccgcaggcct gggcacagat gctgccgacg ttcggcaacc tggtcatcga
     7201 gctcatgaag gccacggcgg tggcctacct catcggtgtc acggacctga cggcggtcgc
     7261 cgatcagatg cgtgaagcca ccggtgagac catcgtggcc ttctccggtg ccctgatcct
     7321 ctactacgtc atcgcgcagg tgctgatctt cggtatgcgc cttctcgaac ggcgggccaa
     7381 ccggaagctg ggacggaccc cgcccggtgg tgcgggactg ttcgctctcc ttcggcctcc
     7441 gaagaccggt acgaaggtgg gggtggcaca gtgacctggg atctccaatt caccctcgac
     7501 tccatgccca ctctgctgga ggggctcctg gtgacggtcc aggtgaccgc gatgggctac
     7561 ctcatcgcgc tctccctggg gctgctgatc gccatcgcac ggcgcgtgcc cgtggcgggg
     7621 tccgtcctgc acgtcgtgat ggagttcgtc cggaccacgc ccctggtggt gcagctggtc
     7681 ttcgtcttct tcctgacccc gcccaacatc tccgggatga ccgtgggtgt cgtcgtcatc
     7741 ggggtgcact actcggccta caccgccgag gtgtaccggt cgggcatcga aggcgtggcg
     7801 aaggggcagt gggaggccgc gcgggcgctc agcctgcctg ccacccggac gtggggggcg
     7861 atcgtgctgc cccaggccat ccggaaggtc atcccggccc tgggcaacta cctcatctcc
     7921 atgttcaagg acacaccgct gctcatcgcg atcggtgtgg ccgagctgct cacccgggcc
     7981 cagcagatcg gctccgcgag cttccgtgtg gtggaggcct acaccatggt cgggttcctc
     8041 ttcctcctgg tgagcatccc gtccgcgatc atcatccgac gactggagag acgctatgcc
     8101 gccgtctgac gccccggaca cagaagaacc ccaggcgggt gagggccagc cgcagatcgt
     8161 cttcgacaag gtggtcaagc gcttcggtga cctcaccgtc ctcgaccacc tcgacttctc
     8221 ggtcgagccc ggcgagcgcg tcaccctcat cggccccagc ggttcgggca agacgaccat
     8281 cctgcgcctg ctcatgaccc tggagaagat caccgagggc cacatccagg tggccggtga
     8341 gccgttcagc cacatgcgcc ggggctccaa gctggtaccc gccagcgagg gctacctgcg
     8401 tgagaagcgc aagagcatcg gcatggtgtt ccagcagttc aacctgttcc cgaacatgtc
     8461 ggtgcgcaag aacgtcaccg aggcgcccgt gcacgtcctc ggcctgccca aggacgaggc
     8521 caacgcgcgg gccgaggagc tgctggacct ggtcgggctg ggggagaaga tcgacgccca
     8581 ccccacccag ttgtccggcg ggcagcagca gcgcgtggcg atcgcccgtg cgctggccat
     8641 gcggccggac atcctgctcc tggacgaggt gacgtccgcg ctggacccgg agctggtggc
     8701 cggtgtcctg gacgtgctgc gccaggtggc cgagaccacc gacatcacga tgctgtgcgt
     8761 cacccacgag atggccttcg ccagggacgt ctcgcaccgc gtgctgatgt tcgacaaggg
     8821 ccggatcgcc gaggagggga ccccggagaa gatcttcgag gacccagagg aggagcgcac
     8881 caaggccttc ctcagctcgg tgctcgacaa cggccactga catagtcgac cgggcggcgg
     8941 ccggggcgcg gggaccgcgc cccggccgcc gcttttggtg ctcttcggct tgccctaacc
     9001 acgacatacc gtagtcatga ggatgctttt agtctctcct tgggcagaat gggtgtcctg
     9061 aggcaggggg gactcttgtg accgagacgc gaagttgggg gctcacctcg gcgggtgtgt
     9121 tcttcgcagt ggtcgtgttc gcgatgctcg ccagtgccag cctccgcgct gagatcctgg
     9181 gcccggacct tcccatcttc ctgacggggt ggggggccga ggtggtcggg gcggtcatgg
     9241 ccttcgtcct cgtccggtgg ttcggcgacc gggacgggct ggacctgagg atccgcctgg
     9301 cgctggagga gcaccccgtc ggccgtgagc tcggcctgct cctcggcttc ctgggcgggt
     9361 tcatcgtgct catggtcctg ctgtcgggga tcttctcgct gctgtcgggg gagatcggtg
     9421 cggacctgcg catgtcggcg gcgatggtct cccggctgct gttcatgctc gccctgccgc
     9481 tgctcttcat ggaccgtgcc gggtacaccg tcagtggtcc gggctccgcc atggccctgc
     9541 tcgtggtgat cgtgcgcgaa ccctggcgct ggctcggcct cattccggtg gtgttcttcc
     9601 tcgtcgtgct cggggtcgcc gccatgcccg cctatggcct gacgggtctc tcactcgctg
     9661 cgctgggtct cgtcctggcc ttcgcgctca tctcggtgtg cgaggagatc ttctaccggg
     9721 gcatggtgca gacccggctg gaactggtca tggggcggtg gggtgggatc ttcgcgaccg
     9781 ccggcctcta ctcgctgagc tacgccctca cccagccgta tgacatcgtg ggccagttca
     9841 gccgtacgga cccggtgtac gacgtcggtc tcgcgctgtg catctacggg tcggtcgccc
     9901 tcttccacgg ctgcctgtgg acgtgctacc gcaacatctg gctgaacgtg ctggtccacc
     9961 tctgcggcct gctcatcctc ctgcggccag aggtaacgtc gcccttgttc tgagagaagt
    10021 cgccttccat acaccctccc ctaacccatg ggaaacatgg tacgaaaatc cctactggat
    10081 gatcgggtgt acgggggctg atgggtacac actccgcata gcagttgtaa gggggtcatc
    10141 ttcccatcat ccgagcgtca cttgatggtc atcacgaggg gggagtctgc caactgtgag
    10201 cacggaatca gcgtccacgc ctgtggaacc caccgccgag gaactcccgc cggatcgctt
    10261 catggaccgc gaggagggct ggctgcggtt caaccaacgg gtcctcgaac tcgccgagga
    10321 cgaggaggtc ccgctcctgg agcgggcgcg tttcctggcc atcttctcca gcaacctcga
    10381 cgagttcttc atggtcaggg tcgccgggct caagcgc
//
