LOCUS       NZ_AZXF01000020        12827 bp    DNA     linear   CON 21-OCT-2024
DEFINITION  Nocardiopsis sp. CNT312 B042DRAFT_scaffold_14.15_C, whole genome
            shotgun sequence.
ACCESSION   NZ_AZXF01000020
VERSION     NZ_AZXF01000020.1
KEYWORDS    WGS; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Nocardiopsis sp. CNT312
  ORGANISM  Nocardiopsis sp. CNT312
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsidaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 128872)
  AUTHORS   Jensen,P., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            assembly :: Velvet v. 1.1.04; ALLPATHS v. R41043
            investigation_type                :: bacteria_archaea
            project_name                      :: Nocardiopsis sp. CNT312
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi14643
            Funding Program                   :: DOE-CSP-ICBG 2011
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. R41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000515115.1-RS_2024_10_21
            Annotation Date                   :: 10/21/2024 21:26:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,296
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 4,144
            CDSs (with protein)               :: 4,144
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 23 of 90
            Pseudo Genes (incomplete)         :: 74 of 90
            Pseudo Genes (internal stop)      :: 5 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:21:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 105228
            Orig. end                         :: 118055
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AZXF01000020.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
FEATURES             Location/Qualifiers
     region          1..12827
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="1"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12827
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12827
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7827](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7827
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    446..460
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.88"
                     /tool="antismash"
     misc_feature    1013..1023
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     misc_feature    complement(1102..1116)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     misc_feature    complement(1685..1702)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 20.11"
                     /tool="antismash"
     misc_feature    2596..2610
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.96"
                     /tool="antismash"
     gene            complement(2789..3784)
                     /locus_tag="B042_RS0110400"
     CDS             complement(2789..3784)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1078: LacI family
                     transcription regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012742.5"
                     /locus_tag="B042_RS0110400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_197024253.1"
                     /transl_table=11
                     /translation="MATAAGVSRSTASRALSGSELISEATRAVVESAALRLGYRPNRAA
                     SALRSRQSRLVGLVMNNLINASFHTIAEVVQKRAAAAGYQVILCITDSDPGKERTVLRM
                     LSEHSVDGLVVIGTGDNADLCDELMVSGTGVVNVIRAPRSGRAPSVLASDREGAHAATR
                     HLLELGHRHIAYVGGPDSANSGRERFAGYQQALREQGLEVDPELVERGPFTPEFGTNAT
                     TALLDRRPDATALFAANHEAVFGILPTLIGRGLRIPHDLSLVCHEDILWLRSWQPPVTV
                     VDNGAAELGRLCFDLLLHQMREGPQPDGTSGRVYRVGANLVERESCTAPP"
     misc_feature    3823..3839
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.56"
                     /tool="antismash"
     misc_feature    complement(3864..3879)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.13"
                     /tool="antismash"
     misc_feature    3910..3927
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.16"
                     /tool="antismash"
     gene            4027..4974
                     /locus_tag="B042_RS0110405"
     CDS             4027..4974
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012904.5"
                     /locus_tag="B042_RS0110405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_028648239.1"
                     /transl_table=11
                     /translation="MDRTDVDWRGYWPAAPTPFDADGAFDEGAWRDLLGLYVDQGVHGI
                     LVNGSTGEWFSQSDAERKRVASSAVREVAGRVPVIVGVSAYTPGGASELARHAAEAGAD
                     GVLATPPPYVHPTSEEVLAFYTEVAAATDLPFMVYNWPRGVAVDLAADQDLIRRLCDIA
                     AVVAVKDSTGDRMRMSATVESVADRARVFGSFLHRWGLAVLAGLGGDGNIDGGGLGAPY
                     AVPAYEAFFAGDTERAAAWIDRYTALSSALVNPDYSGRIASPISQLKAAMGLLGHPGGT
                     VRPPLLPVTAPDDLERLRSALTAAGLTPTRGEDR"
     gene            5001..5249
                     /locus_tag="B042_RS0110410"
     CDS             5001..5249
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF024900.5"
                     /locus_tag="B042_RS0110410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_231493915.1"
                     /sec_met_domain="Fer2_4 (E-value: 3.8e-23, bitscore: 74.1,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIDGRPARIRPGTSVAAALLEQGRWDFRTDPVSGTARGPYCGMG
                     VCLECEVTVDGRPAVRACIERVREGMSIDTSAGEVGP"
     gene            5246..6646
                     /locus_tag="B042_RS22735"
     CDS             5246..6646
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF019604.5"
                     /locus_tag="B042_RS22735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="WP_051415611.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 3.9e-25, bitscore:
                     81.3, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 5.2e-11, bitscore:
                     35.5, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQPTTIRTADAAVVGAGPAGLAAALELGLAGLRVLLLDEQSAPG
                     GQYYRSPGPEAVREVGDHRPAGARLIARVRACGVRVLSGHTVWGVEDDRRGLLAAGPDG
                     DPVIVRADRIVVATGAYERSVPFPGWQLPGVVTPGLAQHMCAEGVRLGRSAVVAGSGPF
                     LLPVACALLERGVRVLAVAEAGRPYTPRPAALGALRFPARLVELASYTARLARHGVRLH
                     QDTVVAEASGRERVREVTLVRRSDPRRVVGRHGADVLCVGYGFRPQSDLAQLLGCDMGR
                     DPATGDPVPLLGAAGRSSVPWVHVVGEAAGVAGAPTALLRGRAAAFDILAARGRTVGRR
                     SRRAVTARLASLARFNGLTSRLYPDPARLTAELAAGLPGDTLVCRCEGVTADTLRSAAC
                     LPGGSDVPALKGSTRAGMGPCQSRECGPALDALRGRGTGAGAVPARVPLRPVPLSTVLH
                     LRAFGEET"
     gene            6643..7827
                     /locus_tag="B042_RS22740"
     CDS             6643..7827
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF013434.5"
                     /locus_tag="B042_RS22740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_155989002.1"
                     /sec_met_domain="DAO (E-value: 4.6e-52, bitscore: 170.3,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGFDAVVIGAGVLGAATALDLAESGLSVLVLERGAPNREGSGTT
                     AGNLHIQAVHTRRPGQAVPLDSQALLPAQKASGQLWAEAEERLDADLEVRRNGGFMVAE
                     TPGQCAELRTKHLWEEQSGLRTEVLDGDAAREALPLLGPTVEAATWCSDDGYANPLKVA
                     PAYLAAARRNAAQVRAFTPVTGLQRRGARWRVETPAGHYDTPVVINVAGPWIGDVCAMA
                     GLRLRMSPVAIQMHATVRERPLLHHLVQHIGEGMSVKQVTSGNLLIGGGWPARRMEQEG
                     RSHASSRSLTGNVAQAARILPVLSGLRLLRMWAGPLAATPDEMPLIGAVPGAPGFYVAG
                     GTYAFTFAPLWGETLGRLIREEQPRVDVSPFSPDRLTETGRASLTPTRKGVTPC"
     gene            7821..9107
                     /locus_tag="B042_RS0110425"
     CDS             7821..9107
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014081.5"
                     /locus_tag="B042_RS0110425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amidohydrolase family protein"
                     /protein_id="WP_028648241.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 4.1e-42, bitscore:
                     137.2, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLNHNRPRTGAGGPGAERSLWLTGGHVVDVVTGAVARDHSVELRD
                     GAIARLTPEPAPPGAEVLDLGGRYLMPGLISVHTHLSVLYPFDAADYGENPATTVLRAA
                     ARARDAVTAGVTTVRTLGEQNRADLLLREAAAKGWAPAPRIVGGGQAISTTGGHGKGGL
                     SVFADGHDEFLKAARTELDAGADHIKIFITGGIAHLGETFNGSQTSREEMRAAVRAAGE
                     HDTYVVAHAGGSQAILEALECGVRGFEHGYDLDDAVVAEMARRRAFLTPTLTVTRCPDW
                     MREHDFTEWQIELAMRVGPGHLESIRRAAAARRTDPADPEAPGITIVTGTDYPPGEPMD
                     GTITQVREMEFLTLAGLSPLEALQAATVNAASLLNLDDRVGRVEEGYAADLIAVGGDPT
                     SDLSVLHDIPLVIQSGNVIRDGITEGVGK"
     gene            9104..11182
                     /locus_tag="B042_RS22745"
     CDS             9104..11182
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014071.5"
                     /locus_tag="B042_RS22745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="WP_036554584.1"
                     /transl_table=11
                     /translation="MTQGNERRVRLAVDIGGTFVDALAYDDSTGEIRINKAPTTPAAPA
                     EGVLRAVAGLADGLGHVSALVHGTTLGLNAILQRRGADVGIVTNEGFRDLFEIARAAIP
                     ADRMYDFSYAPPPILVPRRHRLGVPGRIDAEGRVVTELDEDAVREAGRILVEEHGLASI
                     AVCFLHSYAHPAHEQRAGEILREAFPGVSVSLSTDITREYREYERTSTAVMDAYIRPVL
                     DDYISVLETGLRERGLSDPLHIMRSGGGAMTADLARTAPLTTVLSGPAGGVVGASYLAR
                     ELGIGRLLSFDVGGTSIDSCVIVDGEPSEVHEADIDGFPLLIPIFDLRTIGAGGGSIAW
                     TDDGLLKVGPHSAGAVPGPVAYGQGGTEPTVTDAALALGYLDADEFLGGKMNVDAGAAA
                     TAVRDRLADTLEVDTAEAAASVFRVLMARSVGALREITVERGLDPREFTLLAFGGAGPM
                     LGPMLAREMGIGTTVIPRVPAAFSAFGMLMSDLEYEFSTTVLRPLEARGFAELEPLFAE
                     LEEKANAVLADQGIAESRGHLVRRVDVRYRGQEHTLGIDLAPGDTAASVLASFHTLHRS
                     RYGHAMDEPGEVMTLRVRAVGRLPKPALTAEEPATGPARPHGGRKAFDISTGAMAEFAV
                     HRRERLAPGHRVQGPAIVEEGTATTVLFGDQVLTVDPYGHLLVAASGHVDATGKETP"
     misc_feature    complement(11698..11711)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.32"
                     /tool="antismash"
     misc_feature    complement(11822..11837)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 23.25"
                     /tool="antismash"
ORIGIN
        1 ccgaagcgca gaaggccctg gacacggtcc gcgatctgcg tacacgcctg cttccccgcc
       61 tccgtgaggc cgttcgggcc gcggccgacg cgtgcgggat acgccacccc gccacaccgg
      121 ccgagtgcga gacgacccgc ggactcctcc gcagggtcca ggacaccgtc gaggacctgg
      181 gcccgcaggt gttcgaagcc gacctcgacc gcttcctcca cgccttcagc agggacctgc
      241 gcagaagcga gcccctcgac cccccgctga actggtcgga gcgccgccgg ctgcgccgcc
      301 gcgcacgcgg atggaccacg acgcgcaagt ccgccggaga gtgggcgcag gccctccagc
      361 gcgcccgtgc ggtccggcgg gaatggcacg cagagcgcac cgacggggga gaaccgcgcc
      421 tgatcgatac cctgccggac ctggaggagg ccctcggaca gtgcgtccac gcactcgccg
      481 acctgggacg ggtccttccg cgcggcgtct gcacacacga ggcctccttc accgagctgg
      541 aggcgctcct gacgaggctc tcccgcgaca ccccgggggc gcacggacgc ccccggacga
      601 tggagctgca ctccgccctg gtcagtcgcg gactggaacc cctgctgcgc gaactgcgct
      661 ccccggaggc cagcggtgcc gacggcccgg cggccgtgcg gaggctcgac cacgtctggt
      721 actcctccat tctggagcgg gtcgccgccg gcgattgcgg atacggggcg ttcaacggca
      781 ccagcctgaa caccacggtg gccgagttcg ccgagtccga cggcgagcac ctgcgccgca
      841 acgcccagcg ggtcctgcgc cggaccgccg agagcctgtt cacgatcctg gacgcccacc
      901 cgcaacagca gaccctggtc aagaagcagg cggggctgcg ccgcagccac atgccggtcc
      961 gcgaccttct gggcggggcc gaggagaccc tgctcgcact caaaccctgc tgggccatgt
     1021 caccgctggt ggtgagccag atcctgcccg cacggcggct cttcgacgtg gtggtcttcg
     1081 acgaggccag ccagatcccc gtggccgacg cggtcccggc catcgcccgg gccgacacgg
     1141 tcgtcgtcgc gggtgattcg cgccaactgc cgcccaccgc cttcttcgcc ggagcccggg
     1201 aggaggtcga gggggccgac gacctctcct tcacctccgg cttcgactcc gtcctggagg
     1261 cgctccaccc actgctgccc agccgggcac tgagctggca ctaccgcagc cgcaacgagc
     1321 ggctcatcgc cttctccaac gccaacgtct acgaccgctc actgaccacc ttccccggtg
     1381 cggaggagga gggcgcggtg cgtcacgtcc tggtggagga cacgggggac ccgggccagg
     1441 agaagtcggt caccgccgag gtgcgcaggg tcgtgcggga ggtcatcgca cacgccgagt
     1501 cccgtccgga ggagtccctg ggagtgatca ccatgggcat cgagcacgcg gagaggatcg
     1561 agacggcact cgaccggtcc ctggcccagc ggcgcgacct ggcggggttc ttcgcagagg
     1621 accgccgcga gcgcttcttc gtcaagaacc tggagcgtgt gcagggggac gagcgcgacg
     1681 ccgtcatcct ctccctcgga tacggcaggg gcgccgacgg ccggatgcgt caccacttcg
     1741 ggcccctcaa cctcaagggg ggcgagcgtc ggctcaacgt cgccgtgacc cgggccaaac
     1801 gccggatgac cctggtgagc tccttcggca gccgggacct ggatcccgga cgcctcaacg
     1861 ccgagggcgc caagctcctg cgccgctacc tggagttcgc cgagtccggt ggcagcgacc
     1921 tgggcgcggc cgtggaggag aaccccgaac tcaacccctt cgaggtggac gtacgcgacc
     1981 gtctgtccgc cgcgggcatt ccactgattt gccagtacgg tgtggccggg taccggatcg
     2041 acttcgcggc ggcccatccg cagcgtccgg gccggatggt gctggccgtc gaagccgacg
     2101 gcgcctccta ccactcctcg gtctcggcac gcgatcgaga ccggctgcgc cagcagcagt
     2161 tggagaacct ggggtggacc ttccaccgga tctggtccac cgactggttc cgtgacccgg
     2221 tcagcgaggt ggccagggca cgcctggcct atgaccgggc ggtcgacgcc gccgaccggt
     2281 ccggcgggca ggacaaggcg gagccggccg ctgccggccg tcggcgcgga gaccggggga
     2341 accggacggc cggggagggc gccgacgggt cgacggacgc accctcgacc gcgtctgccc
     2401 ctcaacggag tgaccctgaa ccctctctgc cgccgggcct gacgatcacc gagtaccggc
     2461 cacgggacct ggtcgccctg gcggcctgga tcgagagtga cggactgctg cgcacggagg
     2521 acgaggtcct gcgggaggcc atgcttttcc tgcggtttcg gcgcaagggc aagcgcatcg
     2581 aggacgcgct cacacgggcg atacgttccg cccgtgcccg ccgcggccgt tgaggggtgt
     2641 gcccgctccg ttgcgtgtgc aatgcctgtg aggacacgga tccccgcgat acgcggcacc
     2701 gcgcccgccc gcgtctcacc cctccccggg cgggccgcgg caccgccccg gccggctgcg
     2761 gggcggacgg cgaactcttg ggcggggttc aggggggcgc ggtgcaggac tcgcgttcca
     2821 ccaggttcgc gccgacgcgg tagaccctgc ccgaggtccc gtccggctgc gggccctcgc
     2881 gcatctggtg cagcagcagg tcgaagcaca ggcggccgag ttcggccgcg ccgttgtcca
     2941 ccacggtcac cggcggctgc cagctgcgca gccagaggat gtcctcgtgg cagaccaggg
     3001 acaggtcgtg cggtatccgc agtccccggc cgatcagggt cggcaggatc ccgaagacgg
     3061 cctcgtggtt ggcggcgaac agcgcggtcg cgtccggtcg gcggtcgagg agggcggttg
     3121 tggcgttcgt gccgaactcc ggggtgaagg gaccccgctc gaccagttcg gggtcgacct
     3181 ccagcccctg ctcccgcagc gcctgctggt agccggcgaa gcgctcccgg cccgagttgg
     3241 ccgagtcggg gcctccgacg taggcgatgt gccggtggcc cagctccagc aggtggcggg
     3301 tcgccgcgtg cgctccttcg cggtccgagg ccaggaccga gggggcccgc ccgctccgcg
     3361 gtgcccggat cacgttgacg acgcccgtcc ccgagaccat cagctcgtcg cacaggtcgg
     3421 cgttgtcccc cgtgccgatg acgacgaggc cgtcgacgct gtgctcggag agcatgcgca
     3481 ggaccgtgcg ctccttgccg gggtcggagt cggtgatgca gaggatcacc tggtagccgg
     3541 ccgcggcggc gcgtttttgc acgacctcgg cgatggtgtg gaacgaggcg ttgatcaggt
     3601 tgttcatcac caggccgacg aggcgcgact ggcgggaacg gagcgcactg gcggcccggt
     3661 tgggccggta gccgagcctg agcgcggcgc tctccaccac ggccctggtg gcttcggaga
     3721 tcagctccga cccgctcagg gcccgggacg cggtactccg cgacacccct gccgccgtag
     3781 ccacgtcttt gagagtgacc gccatatctt cccctcgccg aggtgcaact ttatgagatc
     3841 gattgcagga atcctagctc aaagtatccg agtgtgaacc ccggcgaggg gccgtctcct
     3901 cgtttcctca ggttaaaaca atgtttctcc cttgactttc cggtggctcg gtgtcgaaca
     3961 tagtgcaatc gatcccaaag acctgacctg ggatctttgg gacggagaat cggaagaggc
     4021 acgcgcatgg accgcaccga cgtggactgg cgggggtact ggcccgccgc ccccacaccc
     4081 ttcgacgccg acggcgcctt cgacgaaggc gcgtggaggg acctcctcgg tctctacgtg
     4141 gaccaggggg tccacgggat cctggtcaac ggcagcacgg gggagtggtt cagccagagc
     4201 gacgccgaac ggaagcgcgt ggcctcaagc gcggtccgcg aggtggctgg acgcgtcccc
     4261 gtcatcgtgg gggtgagcgc ctacaccccg ggaggggcgt cggaactggc ccgccacgcc
     4321 gccgaggcgg gggcggacgg cgtcctcgcc acccctccgc cctacgtcca ccccacctca
     4381 gaggaggtcc tcgccttcta cacagaggtc gcggcagcca cggacctgcc gttcatggtc
     4441 tacaactggc cccggggcgt ggcggtcgac ctggccgcgg accaggacct catccgtcgg
     4501 ctctgcgaca tcgcggcggt cgtcgcggtc aaggacagca ccggggaccg gatgcgcatg
     4561 agcgccacgg tggagtccgt cgccgaccgc gcgcgcgtct tcggatcgtt cctgcaccgg
     4621 tgggggctgg cggtcctcgc gggtctgggc ggcgacggca acatcgatgg aggcggactg
     4681 ggcgccccct acgccgtgcc cgcctacgag gcgttcttcg ccggggacac ggagcgggcg
     4741 gccgcctgga tcgatcgcta cacggccctg tcctccgcac tcgtcaaccc ggactacagc
     4801 ggtcggatcg cctcccccat ctcccagctc aaggcggcca tgggcctgct cggccacccc
     4861 ggcggcacgg tccgcccccc gctcctcccg gtgacggccc ccgacgacct ggaacggctg
     4921 cgctcggcgc tcaccgccgc cggactcacc ccgacccggg gggaggacag atgaggaagc
     4981 ggaccgcccc gcgtgtatcc gtgaccatcg atggccggcc ggcccgcatc cgcccgggca
     5041 ccagcgtcgc cgcggccctc ctggaacagg ggcgctggga cttccggacc gacccggtgt
     5101 ccggcacggc ccgcggaccc tactgcggca tgggggtgtg cctggagtgc gaggtcaccg
     5161 tcgacggcag accggccgtc cgagcctgca tcgagcgggt gcgcgagggc atgagcatcg
     5221 acacctccgc aggggaggtg ggaccgtgac gcagccgacc acgatccgca ccgccgatgc
     5281 cgccgtcgtc ggggccggcc cggcggggct cgcggccgcg ctcgaactcg gactggcggg
     5341 cctgagggtc ctgctcctgg acgagcagag cgcgcccggc ggacagtact accgcagccc
     5401 cgggcccgag gccgtccggg aggtcggcga ccaccgcccc gcaggggctc ggctgatcgc
     5461 ccgcgtacgt gcctgcggcg tgcgcgtact ctccgggcac accgtctggg gggtggagga
     5521 cgaccgccgc ggcctgctgg cggccgggcc cgacggcgat cccgtgatcg tccgcgccga
     5581 tcggatcgtc gtggccacgg gggcctacga acgctctgtg cccttccccg gctggcagct
     5641 gccgggcgtg gtcacacccg gcctggccca gcacatgtgc gccgagggcg tgaggctggg
     5701 gcgcagcgcg gtcgtcgccg gatccggtcc gttcctcctg ccggtggcct gtgctctcct
     5761 ggagcggggc gtgcgggtcc tggcggtggc cgaggcgggc cggccctaca ccccgcggcc
     5821 cgccgccctg ggcgcgctgc gctttcccgc gcggctggtc gaactggcct cgtacaccgc
     5881 gcgcctggca cggcacgggg tgcgcctgca ccaggacacc gtcgtcgctg aggcctcggg
     5941 ccgcgaacgg gtgcgggagg tcaccctggt acggcgctcc gacccccgcc gggtggtggg
     6001 tcggcacggg gccgacgtgc tctgcgtcgg ctacggcttc cgcccccagt ccgacctggc
     6061 ccagctcctc ggctgtgaca tggggcggga tccggccacg ggcgaccccg ttcccctcct
     6121 cggcgccgcc ggacgcagtt cggtcccttg ggtgcacgtc gtcggtgagg cggccggggt
     6181 cgccggggct cccacggccc tgctccgtgg ccgggcggcg gccttcgaca tcctggccgc
     6241 gcgggggcgg accgtcggcc gccgctcccg cagggcggtc accgcccggt tggcctccct
     6301 ggcccgcttc aacgggctga cctcccgcct gtacccggat ccggcgcgtc tgaccgcgga
     6361 actggccgcc gggctgcccg gagacaccct cgtctgccgc tgcgaaggcg tcacggccga
     6421 caccctgcgc tccgcggcct gcctgcccgg ggggtcggac gtcccggcgc tgaagggctc
     6481 gacccgggcc gggatgggcc cctgccagtc acgcgagtgc gggcccgcac tcgacgctct
     6541 gcgcggccgg gggacagggg ccggggccgt ccccgcacgg gtccccctgc gcccggtgcc
     6601 gctctcgacc gtcctgcacc tgagagcctt cggggaggaa acgtgaccgg cttcgacgcg
     6661 gtggtcatcg gggcgggggt cctgggcgcc gccaccgccc tcgacctggc cgaatcgggc
     6721 ctgtccgtgc tcgtcctgga acgcggagcc cccaacaggg agggatcggg gaccaccgcg
     6781 ggcaacctgc acatccaggc ggtccacacg cgccgcccag gccaggccgt ccccctggac
     6841 agccaagccc tgctccccgc ccagaaggcg tcgggccagc tgtgggccga ggcggaggaa
     6901 cgtctcgacg cggacctgga ggtccgccgc aacggcgggt tcatggtcgc cgaaacaccc
     6961 gggcagtgtg cggaactgcg caccaaacac ctctgggagg agcagagcgg actgcgcacc
     7021 gaggtcttgg acggggacgc cgcgcgggag gcgcttcccc tgctcggccc cacggtcgag
     7081 gccgccacct ggtgctccga cgacggctac gccaatccgc tcaaggtggc ccccgcctac
     7141 ctggcggcgg cccgccgcaa cgcggcgcag gtccgggcgt tcacgcccgt caccggactg
     7201 caacgacggg gggcgcgatg gagggtggag actcccgcgg gccactacga cacccccgtg
     7261 gtgatcaacg tcgcgggccc ctggatcggt gacgtctgcg cgatggccgg cctccggctt
     7321 cggatgtccc ccgtggcgat ccagatgcac gcgacggtgc gggagcggcc cctgctccac
     7381 cacctcgtcc agcacatcgg tgagggcatg tccgtcaagc aggtcacctc gggcaacctc
     7441 ctcatcgggg gagggtggcc cgccaggcgg atggagcagg aagggcgcag ccacgccagc
     7501 agccggagcc ttacgggcaa cgtcgcccag gcggcccgca tcctgcccgt cctctccgga
     7561 ctccgcctgc tgcgcatgtg ggcgggccct ctggccgcga cccccgacga gatgccgttg
     7621 atcggcgcgg tccctggagc accggggttc tacgtcgcgg gggggaccta cgcgttcacc
     7681 ttcgcaccac tgtgggggga gacgctgggc cgactgatcc gcgaagagca gccccgtgtc
     7741 gacgtctccc cgttctctcc cgaccggttg acggaaaccg gacgggcgtc tctcacaccg
     7801 actcggaagg gagtcacacc gtgctgaacc acaaccgacc ccgcacggga gccggagggc
     7861 ccggggcgga gcgatccctg tggctgaccg gcggccacgt ggtcgacgtc gtcaccggcg
     7921 ccgtcgcccg ggaccacagc gtcgaactca gggacggggc catcgcccgt ctcacaccgg
     7981 agccggctcc cccgggggcc gaggtcctcg acctgggggg ccggtacctg atgcccggcc
     8041 tgatctcggt ccacacgcac ctgtcggtgc tctacccctt cgacgccgcc gactacggcg
     8101 agaaccccgc cacgacggtc ctgcgcgccg cggcccgggc ccgcgacgcc gtcaccgccg
     8161 gcgtcaccac cgtgcgcacc ctgggtgagc agaaccgggc cgacttgctg ctgagggagg
     8221 ccgccgccaa gggctgggcg ccggccccgc gcatcgtggg gggcggccag gcgatctcga
     8281 ccaccggagg ccacggcaag ggcggactga gcgtgttcgc cgacggacac gacgagttcc
     8341 tcaaagccgc ccggaccgaa ctggacgccg gcgccgacca catcaagatc ttcatcaccg
     8401 gcggtatcgc ccacctcggg gagaccttca acggttccca gacctcccgg gaggagatgc
     8461 gcgccgcggt gcgggccgcc ggggaacacg acacctacgt cgtggcccat gccggaggtt
     8521 cgcaggccat cctcgaagcc ctcgaatgcg gagtccgggg gttcgaacac ggatacgacc
     8581 tcgacgacgc ggtggtggcc gagatggcac gccgccgcgc cttcctcacc cccacgctga
     8641 ccgtcacccg ctgccccgac tggatgcgcg agcacgactt caccgagtgg cagatcgaac
     8701 tggcgatgcg tgtgggcccc gggcacctgg agagcatccg ccgggccgcc gcggcccggc
     8761 ggaccgaccc cgccgacccg gaggcaccgg gcatcaccat cgtcaccggt accgactacc
     8821 cgcccggcga acccatggac ggcacgatca cccaggtccg tgagatggaa tttctgaccc
     8881 tggcggggct gtccccccta gaggccctcc aagcggcgac cgtcaacgcg gcgtccctgc
     8941 tcaacctcga cgaccgcgta ggacgggtcg aggagggcta cgcagccgac ctcatcgcgg
     9001 tcggcggtga cccgacctcc gatctgtcgg tactccacga catccccctg gtgatccagt
     9061 cgggaaacgt catccgcgac ggcatcaccg aaggagtcgg gaagtgacgc aaggcaacga
     9121 gcgccgcgtg cggctggcgg tggacatcgg gggcaccttc gtggacgccc tcgcctacga
     9181 cgactccacc ggcgagatca ggatcaacaa ggcccccacc accccggccg cgcccgccga
     9241 aggcgtcctg agggcggtcg ccggactggc cgatggactc ggacacgtgt ccgccctggt
     9301 gcacggcacc acgctcggcc tgaacgccat cctccaacgc cgcggggccg acgtcggcat
     9361 cgtcaccaac gaggggttcc gcgacctctt cgagatcgcg cgggccgcca tccccgccga
     9421 ccgcatgtac gacttctcct acgctccgcc gcccatcctg gtcccccggc gccaccgcct
     9481 gggagtgccc gggcgcatcg acgccgaggg gagggtggtc accgaactgg acgaggacgc
     9541 ggtccgcgag gccggccgga tcctcgtgga ggaacacgga ctggcctcca tcgcggtctg
     9601 cttcctccac tcctacgccc accccgcgca cgaacagcgc gcgggagaga tcctgaggga
     9661 ggcgttcccc ggggtgtcgg tgtccctgtc caccgacatc acccgcgagt accgggagta
     9721 cgagcgcacc agcaccgccg tcatggacgc ctacatccga ccggtcctcg acgactacat
     9781 cagcgtcctg gagaccggcc tccgcgagag ggggctgtcg gaccccctgc acatcatgcg
     9841 ctcgggcggc ggcgccatga ccgcggacct ggcacggacg gccccgctga ccacggtcct
     9901 gtccggcccc gccggcggtg tcgtcggagc cagctacctc gcacgcgagc tgggcatcgg
     9961 gcgcctgctg tccttcgacg tcggcggaac cagcatcgac tcctgcgtca tcgtggacgg
    10021 agagcccagc gaggtccacg aggccgacat cgacggcttt ccgctgctca tccccatctt
    10081 cgacctgcgc accatcggcg cgggcggagg ctccatcgcc tggaccgacg acggactcct
    10141 caaagtgggt ccgcacagcg cgggcgcggt ccccggcccc gtcgcctacg gccaaggcgg
    10201 cacggagccc acggtcaccg acgccgcgct ggccctcggc tacctcgacg cggacgagtt
    10261 cctcggcggg aagatgaacg tggacgcggg ggccgcggcc accgcggtcc gcgaccggct
    10321 ggccgacacg ctggaggtgg acacggccga ggccgccgcg agcgtcttcc gcgtcctgat
    10381 ggcccgatcc gtgggcgccc tgagagagat cacggtcgag agggggctcg acccgcgcga
    10441 gttcaccctg ctcgccttcg gcggcgccgg tcccatgctc ggaccgatgc tcgcccggga
    10501 gatgggcatc gggaccacgg tgatcccgcg cgtccccgcg gccttctccg cgttcggcat
    10561 gctcatgtcg gacctggagt acgagttctc caccacggta ctgcgccccc tggaggcgag
    10621 aggcttcgcc gaactggaac cgctcttcgc cgaactggag gagaaggcga acgcggtcct
    10681 ggcagaccag ggcatcgccg agtcccgcgg ccacctcgtg cgcagggtgg acgtgcgcta
    10741 ccggggccag gagcacactc tgggcatcga cctggccccg ggcgacaccg ccgcctcggt
    10801 gctcgcctcc ttccacaccc tccaccggtc gcgctacggc cacgccatgg acgagcccgg
    10861 cgaggtgatg accctgcgcg tgcgggccgt ggggcgactg cccaaacccg cactgacggc
    10921 agaggagccc gccaccggcc ccgcacgccc ccacggaggg cgcaaggcct tcgacatctc
    10981 caccggtgcc atggccgagt tcgccgtcca ccgcagagag cgcctggccc ccggccacag
    11041 ggtccagggc cccgccatcg tcgaggaggg aaccgccacc accgtgctct tcggcgacca
    11101 ggtcctcacc gtggacccct acggccacct gctcgtcgcg gcctccgggc acgtcgacgc
    11161 cactgggaag gagaccccgt gaacaccccg cgaccaccgc tggacggcgc caccgtcgag
    11221 gtcatccgca actacgtcaa ctccgtcgcc gagcagatgc gccgcaccct cgtcaggtcc
    11281 gccttcaacc ccgtcatcta cgacgtgctg gacttcggca tctccatcta cgactccaag
    11341 cgccagctca tggccgaggc cgcgggcatc acccacttcc tgggagccaa cgaccacgcc
    11401 ctggtcaagc tcgtggacta cgtgggagcc gaggcgatgg cccccggcga cgtctacctc
    11461 atgaactacc cctactggag cggtgcccac tcctacgacg ccatgctctg cgcacccgtc
    11521 ttccgggacg ggcacgaggg cccggccgcc tacctcgccg tccgcgccca ctggatggac
    11581 ctgggcgcca aggaggccgg ctacgtcctc gactccaccg acatgcacca ggaaggcatc
    11641 atcttccccg ggacccggat cgtggccggc ggggaggtgg tccgggacgt cgtcgagctg
    11701 atccggttca actcccggct tcccgaggcc accctcggcg atttccacgc ccagttggca
    11761 gccctgcgca cgggggagga gcgcctccgc caggtgtggg acaagttcgg cggcgacacc
    11821 gtcgaacagg ccatcgacct ggtcatcgag cacggagaac gcgtggcccg ccgcgcggtg
    11881 gccaacctcc ccgacgggac ctggcgcgcg gtcgactaca tcgatgacga ctacatcacc
    11941 gacgacctca tccgtatcga ggtcgaggtc agcatcgagg gggaggagat gaccgtcgac
    12001 ttcaacagct catccgcggc cgtggccggg ccggtcaacc ttcccatcgg ttccaccctc
    12061 gggttggcca aggccgcgtt caagggcctg accacagccg acgaggccac caacgcgggc
    12121 cacttccgcc cgctgcgggt cgtcgccgac cccggcaact tcttccacgc cgtctacccg
    12181 tcggccacct tcacacagtg gagtgcgatc gtcgccttcg aactggtcta caaggccctg
    12241 gcacgggtca tccagaccct gccggcctcc agcggaggcg acgaacccgg gttcatggcg
    12301 ctggggaacg acccgcggac ggggcgggac tacgtggtca gcaacaacga gggcatcgga
    12361 tggggcgcgg cccgcgacca cgacgggggc accgcccaac agcacccctc acagaccacg
    12421 gtccgcaaca caccgatcga ggtcttggag cacaaggccg ccctcttcca cgagaagctc
    12481 gaactcctcc ccgactcggg aggccccggc cgccaccgcg gcgggttcgg agtcgagcgc
    12541 gtcgtgcgct acaccgcgcc cggcgaggtc ctgtccatga agaagaagag caagacccgg
    12601 ccctgggcgc tgcacggcgg acacgaacca gaacccagcc acatggagct gtggccggac
    12661 accgaccgca gcaggagggt cggcatgtac cgggcacgga tggagcccgg cgagcgcttc
    12721 gccaaccgca ccgcgggggg aggcggctac ggccacccct tcgagcgcga ccccgcggcc
    12781 gtggtggagg acgtgctcaa cggactcgtc accgccgaag cggcccg
//
