LOCUS       NZ_AZXF01000018         8151 bp    DNA     linear   CON 21-OCT-2024
DEFINITION  Nocardiopsis sp. CNT312 B042DRAFT_scaffold_12.13_C, whole genome
            shotgun sequence.
ACCESSION   NZ_AZXF01000018
VERSION     NZ_AZXF01000018.1
KEYWORDS    WGS; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Nocardiopsis sp. CNT312
  ORGANISM  Nocardiopsis sp. CNT312
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsidaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 149806)
  AUTHORS   Jensen,P., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            assembly :: Velvet v. 1.1.04; ALLPATHS v. R41043
            investigation_type                :: bacteria_archaea
            project_name                      :: Nocardiopsis sp. CNT312
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi14643
            Funding Program                   :: DOE-CSP-ICBG 2011
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. R41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000515115.1-RS_2024_10_21
            Annotation Date                   :: 10/21/2024 21:26:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,296
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 4,144
            CDSs (with protein)               :: 4,144
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 23 of 90
            Pseudo Genes (incomplete)         :: 74 of 90
            Pseudo Genes (internal stop)      :: 5 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:21:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 141655
            Orig. end                         :: 149806
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AZXF01000018.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
FEATURES             Location/Qualifiers
     region          1..8151
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..8151
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..8151
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5309](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5309
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    44..58
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.38"
                     /tool="antismash"
     misc_feature    complement(660..675)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.63"
                     /tool="antismash"
     gene            1123..1926
                     /locus_tag="B042_RS0109280"
     CDS             1123..1926
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (7-164). E-value: 1.8e-13. Score:
                     42.8. Matches aSDomain:
                     nrpspksdomains_B042_RS0109280_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017599384.1"
                     /locus_tag="B042_RS0109280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_028648060.1"
                     /sec_met_domain="adh_short (E-value: 7.4e-31, bitscore:
                     100.6, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDTAARTALVTGASSGIGATVAAALAGRGYRVYGTSRSPGSVPD
                     PVPGVEYLALDLADQSSIEACAEAAGAVDVLVNNAGESQSGPFEELPPEALERLFRVNV
                     LGQVRLTQLLLPGMREHGYGRVVMVGSMLASFPLAYRSSYVASKAALKGFSDALRREVS
                     PYGVRVTTVEPGSINTGISERRTQYLREGSPFAEEYRTMPGRLNANEAAGISAETVAET
                     VLRAVGADRPRPLYAVGSSAPGVFALRRLLPYATVLRMVARRHGL"
     aSDomain        1144..1614
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B042_RS0109280_PKS_KR.1"
                     /evalue="1.80E-13"
                     /label="B042_RS0109280_PKS_KR.1"
                     /locus_tag="B042_RS0109280"
                     /protein_end="164"
                     /protein_start="7"
                     /score="42.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGASSGIGATVAAALAGRGYRVYGTSRSPGSVPDPVPGVEY
                     LALDLADQSSIEACAEAAGAVDVLVNNAGESQSGPFEELPPEALERLFRVNVLGQVRLT
                     QLLLPGMREHGYGRVVMVGSMLASFPLAYRSSYVASKAALKGFSDALRREVSP"
     CDS_motif       1150..1212
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B042_RS0109280_0001"
                     /evalue="9.70E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="B042_RS0109280"
                     /protein_end="30"
                     /protein_start="9"
                     /score="11.8"
                     /tool="antismash"
                     /translation="LVTGASSGIGATVAAALAGRG"
     gene            1992..3221
                     /locus_tag="B042_RS22540"
     CDS             1992..3221
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024700.5"
                     /locus_tag="B042_RS22540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AAA family ATPase"
                     /protein_id="WP_197024230.1"
                     /transl_table=11
                     /translation="MARRRLRSLTVEGLTSIRSATVELGDVNVLIGANGAGKSNFVSAL
                     AMLGRIADGDLGLFVGLNGGASGLLHQGPDRAAEIRLRAAIESDAQRNQCVSRDHGFTY
                     KATLTEARDDRLIFSGEEIIRSTGSEHPLRVKGGHGESVLSEAAASAVPSSDAEALTAI
                     ADILRGCRVFHFHDTGLNAPMKRIGYAADNLTLHPDAGNLAALLMRLRESEPYAYRRIL
                     RDIRKGAPFLRDFVLVEQNEQLQLRWKQVDSDTVFPAGALSDGTLRFICLATLLGMPNP
                     PGIIVLDEPELGLHPYAIVQLADMLRAAGTSSQIVIATQSTTLVDQFALDELVIVERER
                     GPPNSAVRPPVPCVCGWTTTPWANCGRRTCSAAAPVPSEGDRAQAPHPLRGPDRGAAHR
                     GHPQPVLHHP"
     misc_feature    2038..2052
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.92"
                     /tool="antismash"
     gene            3314..3802
                     /locus_tag="B042_RS22545"
     CDS             3314..3802
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025468.5"
                     /locus_tag="B042_RS22545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4276 family protein"
                     /protein_id="WP_051415564.1"
                     /transl_table=11
                     /translation="MRSGICSITVLSTLIDYYGVPSDTPGMATRPPGTPRSQVEHVEAA
                     MSEAVGEPRFLPHLVVHETEAWVLACPEVLSELVGAPKLAGRIRALTVSSGGPELVNDG
                     PDTAPSKRLRSLVPGYRKTVDGPDAIGLTGVDAVRRQCPHADAWFHAVDERLGTIRRS"
     gene            3905..4333
                     /locus_tag="B042_RS0109295"
     CDS             3905..4333
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154530.1"
                     /locus_tag="B042_RS0109295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="VOC family protein"
                     /protein_id="WP_028648061.1"
                     /transl_table=11
                     /translation="MQQQVHFITLATADLDAARRFYRDGLGWEPLVDVPGEIVFFQVAP
                     GTVLGLFDAEKFGRDLGGTGAPGVSGVTLSHNVGGPREVEETVALMAAAGGTVLKAPRQ
                     GEFGEVFHAHARVPNGVVWEVARHPGWLVAEDGRAVLG"
     gene            4406..4762
                     /locus_tag="B042_RS0109300"
     CDS             4406..4762
                     /EC_number="1.14.-.-"
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017537161.1"
                     /locus_tag="B042_RS0109300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="WP_028648062.1"
                     /transl_table=11
                     /translation="MSVVKFNVLTVPEGSGATLEERFAKRAGLVEDQPGFEEFQLLRPV
                     EGTEKYLVYTRWSSEEDFQNWVSGQDFKRGHAQAAKDAGQAGHGHGGEGGARPAATGSE
                     LWGFEVVQRVSARG"
     misc_feature    4957..4969
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.78"
                     /tool="antismash"
     gene            5001..5309
                     /locus_tag="B042_RS0109305"
     CDS             5001..5309
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021219.5"
                     /locus_tag="B042_RS0109305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_028648063.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 9.4e-15,
                     bitscore: 48.1, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGTNIKRAAATAAITAALATAAAAPAYAVIEYVGGGVWDHGFKGT
                     FGGTVYSSYYHGGCGHSATAVGDDLVRDYAPKGYTAYAEVSSAAWGNATYWNRCVTS"
     gene            5385..7481
                     /locus_tag="B042_RS0109310"
     CDS             5385..7481
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B042_RS0109310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_028648064.1"
                     /transl_table=11
                     /translation="MLHRSILVIHLALLFIAAFFSFLTVRSYEEMTTTVPAGVVWASSN
                     DQTANHSQITETVEGFARETGVSVALQVPDLRDPGSVRHLYLAPGREGALASDWLEDGY
                     PVFGHTMDTRVHPFGELDRDDPRGFYLVFGTEADTDAFEATLAGIGLHDTNSEGGYALR
                     WTDLFRLGGDLSFALIITVLCGITVTGSGVLLNARNYGVLRLQGYSYTRILARDLVQIG
                     RFYLLAGGCTAVGITAALLLYNGLAQFALFARIAGWLLALFLGTALLTHAFALALLQAT
                     SLLDALKGRIPARPATASAYAVRVPALVLVLVSVSLVVHSAQNLREHRKEFDTYSEAGQ
                     ASRITLSGSVDFAEVGESMDPLVGGWLRQADEEGQIILSVRQQSAILAPPGEPGPGADL
                     LVVNDTYLEHQEILSPDGERYGPSDTVRVLLPQSLAEWGETVAQSAGEQAASWNSFGRP
                     AGGDPQRFDTEVLPTADGQEVFTYGSDGPVGRPRVPFLDDPMIFVLPDGAVLSDQLYVT
                     FATQAGIVFPDPEEVEESIATPPFSTYINGLQMVVTSLADDYAEQVRHLRLQSFNLAAA
                     TGVLVMTAVAVCLVHVRTRAQTIFAKHISGWRFTAIHRRLLVAEGALALAFLGWAALRA
                     FRVLIRVDGPTAPGTLAPTPEQAAVAALEPLLAIAIAGGGLLLIAAILLHFHRRIVREG
                     AAQA"
     gene            7494..8129
                     /locus_tag="B042_RS0109315"
     CDS             7494..8129
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546332.1"
                     /locus_tag="B042_RS0109315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_028647700.1"
                     /transl_table=11
                     /translation="MITVRDLSKSFGARTLWRGLDLDLPPGRMLALTGPSGAGKTTLLN
                     CLGLLEAPTGGSISHGGRDLTRLRAGGRRRFRRDHLGYLFQNYALMENATVRENLDVAR
                     RDRPAQAEALERVGLAGRSRERVHHLSGGEQQRVALARLLVKQPSLVLADEPTGALDEG
                     NGQMVVDTLRAMAAEGCSVVIATHNSAVREACDTVLDVSTRQPATERA"
ORIGIN
        1 aggggcgtgg gctggccggt ctacgccatg gtcgcggtcg gcctggccat catcttcggc
       61 ctgcccaggc tgaccaccgt cgtgcccgcc ccgctggtgg cgatcgtggt actgaccgcc
      121 gcggccctcg ccctgggcat cccggccagc acggtcgggg acatgggcga gctgcccgac
      181 agcgtcccct tcccgttcct gccggacgtg cccctgaccg tggagaccct cgccctcatc
      241 gcgccgttct ccctcacgct ggcgctggtg gggctgatgg agtcgctgat gaccgcgaag
      301 gtcgtcgacg aacggaccga gacctcctcg gaccactcac gcgaggcacg cggacagggc
      361 atcgccaacg tgatcgtggg cttcttcggc ggcatggccg ggtgcgcgat gatcggccag
      421 acgatgatca acatctcctc gggtgcccgc acccgggtct cgaccttcct ggccggggtg
      481 ttcctgctga tcctgtgcgt ggtgctgggc gacctcgtgg gcatgatccc gatggccgca
      541 ctggtcgcgg tgatggtctt cgtgtcggtg gtgaccttcg actggcacag cgtcgccccc
      601 aagacggtgc ggcggctgcc ctggaccgag acgctggtca tggtcaccac ggtcgcggtg
      661 gtggtctaca cccacaacct ggccctgggc gtgatcgtcg gtgtgatcgt gtcgatggtg
      721 ctcttcgccc agaaggccgc cacccacgcc gacgtcacca gcgtcctgga ccccgagggc
      781 ggcacccgcg tgtactcggt gcacggcgag ctgttcttcg cctccaccaa ccagctggtg
      841 ggccggttcg actacgccga gaaggacctg tccaaggtgg tcgtggacat gtcggaggcc
      901 cacgtgtggg actcctctgc ggtcgcggcg ctcgaccacg tctccgaaca cttccgcaag
      961 cacggcgtcg aggtcgagat caccggcctc aacgagcgga gccggcagtt gcacaaggag
     1021 ctgaccggca ccctgaccgg cggccactga cggcacggag gtaggaaggg ggcacgggcc
     1081 gtcagcggcc cgcgccccct tcgccgtctc cgaggaatct caatgaccga caccgccgcc
     1141 aggaccgcac tcgtgacggg agcctcctcc gggatcgggg ccaccgtggc cgccgccctg
     1201 gccgggcgcg gataccgcgt ctacggcacc agccgctccc ccggttcggt ccccgatccg
     1261 gttcccgggg tggagtacct ggccctggac ctggccgacc agtcctcgat cgaggcgtgc
     1321 gcggaggccg ccggagcggt ggacgtgctg gtcaacaacg ccggggagag ccagagcggc
     1381 cccttcgagg agctgccgcc cgaggccctg gaacggctct tccgggtcaa cgtgctcgga
     1441 caggtccggc tgacccagct cctgctcccg gggatgcgcg aacacgggta cggccgggtg
     1501 gtgatggtgg gctccatgct ggcgagcttc ccgctggcct accggtcctc ctacgtggcg
     1561 agcaaggcgg cgctcaaggg gttctccgac gccctgcgcc gcgaggtgtc gccctacggg
     1621 gtccgggtga cgacggtgga gccggggtcg atcaacaccg ggatcagcga gcggcgcacc
     1681 cagtacctga gggagggctc gccgttcgcc gaggagtacc gcaccatgcc gggcaggctc
     1741 aacgccaacg aggcggcggg catctccgcg gagaccgtcg cggagaccgt tctgcgcgcg
     1801 gtcggcgccg accggccgcg ccccctctac gccgtgggca gcagcgcgcc gggggtgttc
     1861 gccctgcgcc gcctgctgcc gtacgcgacg gtgctgcgga tggtcgcccg caggcacggc
     1921 ctctgagggc accggggcgg cgggccgggc agcgtatcca ggttcggccg ccgcctggct
     1981 agggctgacc catggcacgc cgcagactcc gctccctcac cgtcgaagga ctcacctcga
     2041 tccgttcggc cacggtcgag ctcggggacg tgaacgtgct catcggcgcc aacggcgcgg
     2101 gcaagagcaa cttcgtctcg gcactggcca tgctggggcg tatcgccgac ggtgacctgg
     2161 gactcttcgt cggcctgaac ggtggggcgt cagggctgct gcaccagggg ccggaccgcg
     2221 ccgccgagat caggttgcgg gccgcgatcg aatccgacgc gcagcggaac cagtgcgtat
     2281 cccgtgatca cggcttcacc tacaaggcga ccctgaccga ggctcgcgac gaccgcttga
     2341 tcttcagcgg tgaagagatc atccggtcca ccggctcgga acaccctctg cgtgtgaagg
     2401 gagggcacgg ggagagcgtg ctctccgagg ctgccgcctc cgccgtcccc tcctctgacg
     2461 cggaggccct cacagccatc gccgatatcc ttcgcggttg ccgtgtcttc cacttccacg
     2521 acaccggttt gaacgccccg atgaagcgca tcggctacgc ggccgacaac ctcaccctgc
     2581 accctgacgc gggcaacctc gccgcactgc tcatgcgctt gcgcgagtcc gagccgtacg
     2641 cgtaccggcg catcctgcgc gacatccgca agggcgcacc gttcctccgg gacttcgtcc
     2701 tcgtcgagca gaacgaacag ctacagctgc gttggaagca ggtggacagc gacaccgtgt
     2761 tccccgccgg cgccctgtcc gacggaacac tgcgcttcat ctgcctggcc accctgctcg
     2821 gtatgccgaa ccctcccggc atcatcgtcc tggacgagcc cgaactcggc ctgcacccgt
     2881 acgccatcgt ccaactcgcc gacatgctgc gggccgctgg aacctccagc cagatcgtca
     2941 tcgccaccca gtcgacgacg ctcgtggacc agttcgcgct ggacgaactg gtcatcgtcg
     3001 aacgtgagcg ggggcctccg aattccgccg tccggccccc ggtcccctgc gtgtgtggct
     3061 ggacgactac tccctgggcg aactgtggca gaagaacctg ctcggcggcc gcccccgttc
     3121 cgagcgaagg tgaccgtgca caggctccac atcctctgcg aggaccagac cgaggagcag
     3181 ctcaccgagg acaccctcag cccgtacttc accacccctg acacctacgt gagctggtcg
     3241 gtcatcacca cgaagcgcac gacgagcggg ggtgacgtca agggaggggt cggtagatgg
     3301 ggcgccctgc accgtgagat ccggaatctg ctccatcacc gtcctcagca ccctcatcga
     3361 ctactacggt gtgccctcgg acactccggg tatggcgaca cgcccgccgg gcacaccccg
     3421 gtcacaggtg gagcatgtgg aagcagcgat gtcagaggcc gtcggcgaac cgcgcttcct
     3481 gccacacctg gtggtgcacg agaccgaggc atgggttctg gcgtgcccgg aggtcctgtc
     3541 cgaactcgtc ggcgcaccga aactcgccgg tcggatccga gccctcacgg tcagctcggg
     3601 aggccccgaa ctcgtcaacg acggacccga caccgctcct tccaaacggt tgcgttcact
     3661 ggtgcccggc taccgcaaga cggtggacgg tcctgatgcc ataggcctca ccggggtgga
     3721 cgcggtccgg cggcagtgtc cgcacgccga cgcctggttc cacgccgtgg acgaacggtt
     3781 ggggaccatc cggcgctcct gacatgcccg caccgggcgc gacacggggg gcgggttcct
     3841 ccggcatgtc ggcggccacg gtcaggatgg ggaccgcccc gatcccggac gaggagaccg
     3901 ccccgtgcaa cagcaggtcc acttcatcac cctggccacc gccgacctgg acgcggcccg
     3961 gcgcttctac cgcgacgggc tcggctggga gccgctggtg gacgtccccg gtgagatcgt
     4021 cttcttccag gtggcgcccg gcacggtcct ggggctgttc gacgcggaga agttcggccg
     4081 ggacctgggc ggtaccggcg cgcccggggt gtcgggggtg acgctctcgc acaacgtcgg
     4141 cggcccgcgg gaggtggagg agacggtggc cctgatggcc gccgccgggg ggacggtcct
     4201 caaggcgccg cggcagggcg agttcggcga ggtcttccac gcccacgcgc gggtccccaa
     4261 cggggtggtg tgggaggtgg cccgccaccc ggggtggctc gtcgcggagg acgggagggc
     4321 cgtgctcggc tgagtcggac ggtgcaccgg acggaggcgg gtctaggttg ggcggtgcag
     4381 tcccagtcca cgtgagaggt gcgcgatgag cgtcgtcaag ttcaacgtcc tgactgttcc
     4441 cgagggctcg ggggccaccc tggaggagcg cttcgccaag cgcgcgggcc tggtggagga
     4501 ccagcccggt ttcgaggagt tccagctcct gcgcccggtc gagggcaccg agaagtacct
     4561 ggtgtacacg cggtggagtt cggaggagga cttccagaac tgggtgagcg gccaggactt
     4621 caagcgcggc cacgcccagg cggcgaagga cgccgggcag gcgggccacg ggcacggcgg
     4681 tgagggcggt gccaggcccg ccgcgaccgg cagcgagctg tggggcttcg aggtcgtcca
     4741 gcgggtctcc gcccggggct gagcctttcc ggcgcggggt cggccggtgc gcaccggccg
     4801 accccgggat tgcccccatg gccggatgtg gccacggcgc acacaacaaa gacgaaggcc
     4861 cagatgagac gcaataccac gcacattgtg gcgtgttttg cgacctaatg tccgcttgtc
     4921 tgcttttttg ggggggtagt ttctgcgtcg gttcacacgc agtgtgcaac cagaaacaac
     4981 cccctcacag gagaatagtc atgggcacca acatcaagcg ggccgctgcc accgcggcca
     5041 tcaccgccgc gctggcgaca gcagcggcgg ccccggccta cgccgtgatc gagtacgtgg
     5101 gcggcggagt gtgggaccac ggtttcaagg gcaccttcgg tggcaccgtg tactcaagct
     5161 actaccacgg cggttgcggt cacagcgcga ccgcggtagg tgatgacctg gttcgcgact
     5221 acgctccgaa gggctacacc gcctacgccg aagtctcttc cgccgcgtgg ggcaacgcga
     5281 cctactggaa ccgctgcgtg acctcctgaa cccgcgagca tgtcccgagg ccgtcagcgt
     5341 ccccgcgttg acggcctccc gccgccgacc agcccggagc ccccgtgctt caccgaagca
     5401 tcctcgtcat ccacttggcg ctgctgttca tcgccgcctt cttctccttc ctcacggtcc
     5461 gctcctacga ggagatgacg accacggtcc ctgcgggagt cgtctgggcc agcagcaacg
     5521 accagaccgc gaaccactcc cagatcaccg agaccgtcga gggcttcgcc cgggagacgg
     5581 gggtctccgt ggcgctccag gtgcccgacc tgcgcgaccc cggttccgtg cgccacctct
     5641 acctggctcc gggccgggag ggcgcgctcg cctcggactg gctggaggac ggctatccgg
     5701 tgttcgggca caccatggac acccgggtcc accccttcgg ggaactggac cgggacgacc
     5761 cccggggctt ctacctcgtc ttcggcaccg aagcggacac cgacgccttc gaggccacac
     5821 tcgccggtat cggtctgcac gacaccaaca gtgagggcgg atacgccctc aggtggaccg
     5881 acctcttccg cctcgggggc gatctcagct tcgccctcat catcaccgtg ctgtgcggta
     5941 tcaccgtcac cggctcaggg gtgctgctga acgccaggaa ctacggggtg ctccggctcc
     6001 agggatactc ctacaccagg attctggcgc gggacctggt gcagatcggg cgtttctacc
     6061 tcctggcggg tggttgtacg gccgtgggga tcacggccgc gctcctgctc tacaacgggc
     6121 tcgcccagtt cgcactcttc gcccggatcg cgggatggct gctggccctg ttcctgggca
     6181 ccgcccttct tacccatgcc ttcgccctgg cactgttgca ggccacgagc ctcctcgacg
     6241 cgctcaaggg aaggatcccc gccaggccgg ccaccgcgag cgcctacgcc gtacgggtcc
     6301 cggccctggt cctggtcctg gtctcggtga gcttggtcgt ccactccgcc cagaacctgc
     6361 gggagcaccg gaaggagttc gacacctact ccgaggccgg gcaggcctca cgcatcacgc
     6421 tctccggcag tgtggacttc gccgaagtgg gtgaatccat ggacccgctc gtgggcggct
     6481 ggctgcgcca agccgacgag gagggacaga tcatcctgtc cgtacgtcag cagtccgcga
     6541 ttctggcccc accgggcgaa ccgggtccgg gagccgacct gctcgtggtc aacgacacct
     6601 acctggaaca ccaggagata ctctcccccg acggcgagcg ctacggcccc tcggacacgg
     6661 tccgcgtcct tctcccgcag tccctcgccg agtgggggga gaccgtggca cagtcagccg
     6721 gggagcaggc cgcctcctgg aacagcttcg gccgaccggc cgggggcgat ccccagcggt
     6781 tcgacaccga ggtgctgccc accgccgacg ggcaggaggt gttcacctac ggttccgacg
     6841 ggccggtggg acggccccgg gtccccttcc tcgacgaccc gatgatcttc gtcctccccg
     6901 acggcgcggt gctctccgac cagctctacg tcaccttcgc gacacaggcc gggatcgtct
     6961 tccccgaccc cgaggaggtg gaggagtcca tcgccactcc gcccttctcc acgtacatca
     7021 acggtctcca gatggtcgtc accagcctcg ccgacgacta cgccgaacag gtccgtcacc
     7081 tgcggctgca gtccttcaac ctggccgcgg ccacgggggt gctggtgatg accgcggtcg
     7141 cggtgtgcct ggtccacgtc cgcacccggg cccagacgat cttcgcgaaa cacatcagcg
     7201 gttggcggtt caccgccatc caccgccgac tgctggtcgc ggagggcgcc ctggcgctgg
     7261 cgttcctcgg ctgggcggcc ctgcgggcct tccgcgtcct gatccgtgtc gatggcccaa
     7321 ccgccccggg caccctcgca cccacccccg agcaggccgc cgtcgccgct ctggaaccgc
     7381 tcctggccat cgccatcgcg ggcggcgggc tcctgctcat cgccgcgatc ctcctgcact
     7441 tccaccgccg tatcgtccgt gagggcgccg cccaggcctg aaagggatct gccgtgatca
     7501 ccgtccgcga cctgtccaag tccttcggcg cccgcaccct gtggcggggc ctggacctcg
     7561 acctgccccc cgggcggatg ctcgccctga ccggcccctc cggggcgggc aagaccaccc
     7621 tgctcaactg cctgggcctg ctggaggccc ccaccggcgg cagcatcagc cacggcggac
     7681 gcgacctcac ccgcctgcgg gccggcgggc ggcgccgctt ccgccgcgac cacctgggct
     7741 acctcttcca gaactacgcg ctgatggaga acgccacggt gcgcgagaac ctggacgtgg
     7801 cccgccgcga ccggcccgcc caggccgagg ccctggagcg ggtggggctg gccggccgct
     7861 cccgtgagcg ggtgcaccac ctctcgggcg gcgaacagca gcgggtggcc ctggcccgcc
     7921 tgctggtcaa gcaaccgtcc ctggtgctgg ccgacgagcc caccggcgcc ctggacgagg
     7981 gcaacggcca gatggtggtc gacaccctgc gcgccatggc cgccgaaggg tgttcggtgg
     8041 tcatcgccac ccacaacagc gccgtgcgcg aggcctgcga caccgtcctc gacgtctcca
     8101 cccggcaacc ggccaccgaa cgggcctgac cccgggcggg accctgcccg g
//
