LOCUS       NZ_AZXF01000015         8135 bp    DNA     linear   CON 21-OCT-2024
DEFINITION  Nocardiopsis sp. CNT312 B042DRAFT_scaffold_9.10_C, whole genome
            shotgun sequence.
ACCESSION   NZ_AZXF01000015
VERSION     NZ_AZXF01000015.1
KEYWORDS    WGS; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Nocardiopsis sp. CNT312
  ORGANISM  Nocardiopsis sp. CNT312
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsidaceae; Nocardiopsis.
REFERENCE   1  (bases 1 to 165205)
  AUTHORS   Jensen,P., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            assembly :: Velvet v. 1.1.04; ALLPATHS v. R41043
            investigation_type                :: bacteria_archaea
            project_name                      :: Nocardiopsis sp. CNT312
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi14643
            Funding Program                   :: DOE-CSP-ICBG 2011
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. R41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Unknown
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000515115.1-RS_2024_10_21
            Annotation Date                   :: 10/21/2024 21:26:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,296
            CDSs (total)                      :: 4,234
            Genes (coding)                    :: 4,144
            CDSs (with protein)               :: 4,144
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 56
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 23 of 90
            Pseudo Genes (incomplete)         :: 74 of 90
            Pseudo Genes (internal stop)      :: 5 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:21:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 157070
            Orig. end                         :: 165205
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AZXF01000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
FEATURES             Location/Qualifiers
     region          1..8135
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..8135
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..8135
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5330](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5330
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(334..984)
                     /locus_tag="B042_RS0107070"
     CDS             complement(334..984)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017597585.1"
                     /locus_tag="B042_RS0107070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_028647695.1"
                     /transl_table=11
                     /translation="MDAHTVDGRTARSRATRARVATAAARLFTERGYAATSIQSIAAEA
                     GVAAQTVYYAFGTKSAVLKHALDHAVAGDAAPVATLDRPWVRRALEAPDARGQIRLHTV
                     GTASVMERLSPLSEVLRGAADSDDELRALWHASVDQRRTVQRTFTERLRERGALRPELS
                     ADEAADTCLALLSPEVFILYTRHCGWSVRRWRTWAEQGLTRHLTELSTAPEQG"
     misc_feature    1030..1046
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.41"
                     /tool="antismash"
     misc_feature    complement(1036..1049)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.86"
                     /tool="antismash"
     gene            complement(1214..2164)
                     /locus_tag="B042_RS0107075"
     CDS             complement(1214..2164)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013152601.1"
                     /locus_tag="B042_RS0107075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_028647696.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 4.3e-24, bitscore:
                     78.4, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTRHGVDVAVIGGGAAGLSAAVTLARSLRSVVVVDGGEPRNAPA
                     AGAHNVLGHEGIAPRDLLTAGRAEADRYGARFHDGRAVAARHCEGAFEVDLDDGGTLRA
                     RRILLATGLVDELPDVPGVRDLWGTDVLHCPYCHGWEVRGRRIGVLGTSELSLHQALMF
                     RQLSEHVTLFTHTMGSPEDQAWEQMAALDVQVVEGPVRMLSTEGGRLRAVVLAEGHAFP
                     VDAVAVAPRFTARTDLYEQLGGAAADHPMGSFIATGPMGRTDVEGVWAAGNAADLSAMV
                     SVSTGAGVAAAAAINADLVAQDARAAVRARRAAAS"
     gene            complement(2283..3419)
                     /locus_tag="B042_RS0107080"
     CDS             complement(2283..3419)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017611701.1"
                     /locus_tag="B042_RS0107080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_028647697.1"
                     /transl_table=11
                     /translation="MDLGFLRPLYESDAPIASVHLDTSRESRDADKKIELRWRHLRATL
                     DALGADQATLNALETAIVEHNSRSFGTHGQALFASQGELISAYTLTEPPDQDRATWLPV
                     PDALPVAIDRGRHLPYVVVALDRVNAHITAYPAYPAEGPASEQDLTGASLRGLDVTSRG
                     GPGNLGGYNGRFPGKHVAQELWRENTAKVAHHVREAVADVAADVVFVGGDDEAIAYLRD
                     NLGERRTELPIVVLPGGRGGTDAEERLHQAAAEALHQFVTSDQERVAADFRSKLAHEQA
                     VAGTEPTLPMLSEARVRTLLMGAERDAEPHLWGSRTEPVLVAAQRTDLDRPEAAFRAPA
                     SALMLRSAVMTDSDFTELLEDGEPTTDSGAILRFADPS"
     gene            complement(3586..4437)
                     /locus_tag="B042_RS0107085"
     CDS             complement(3586..4437)
                     /NRPS_PKS="Domain: Abhydrolase_1 (32-263). E-value:
                     1.6e-28. Score: 91.9. Matches aSDomain:
                     nrpspksdomains_B042_RS0107085_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017543331.1"
                     /locus_tag="B042_RS0107085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_028647698.1"
                     /sec_met_domain="PF00561 (E-value: 7.9e-23, bitscore: 74.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 6.2e-23, bitscore:
                     75.7, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPLTTNAAGSDRPNTAPVRGINVGYSDRGTGYPLLMIHGHPFDR
                     TMWTPQTEFMSAHGYRVIAPDLRGYGTSTVVPGTTTLEVFARDLAALLDHAGADTVGVV
                     GLSMGGQVALELYRLFPDRVDSLVLAATDPHPEAERRRLERRRLAALLRAEGMAGYADR
                     MLVGMMSADNVRRSPDTARHVHTMMRGTDPEGAAAALLGRALRPDYVPLLKRVSCPTLL
                     VAGQHDTLTSPGLTEAMHVLVPDSVVEIIEGAAHLPNLERPQAFNRAVHRFLRDRTCSS
                     ER"
     aSDomain        complement(3649..4341)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B042_RS0107085_Abhydrolase_1.1"
                     /evalue="1.60E-28"
                     /label="B042_RS0107085_Abhydrolase_1.1"
                     /locus_tag="B042_RS0107085"
                     /protein_end="263"
                     /protein_start="32"
                     /score="91.9"
                     /tool="antismash"
                     /translation="YPLLMIHGHPFDRTMWTPQTEFMSAHGYRVIAPDLRGYGTSTVVP
                     GTTTLEVFARDLAALLDHAGADTVGVVGLSMGGQVALELYRLFPDRVDSLVLAATDPHP
                     EAERRRLERRRLAALLRAEGMAGYADRMLVGMMSADNVRRSPDTARHVHTMMRGTDPEG
                     AAAALLGRALRPDYVPLLKRVSCPTLLVAGQHDTLTSPGLTEAMHVLVPDSVVEIIEGA
                     AHLPNLERP"
     misc_feature    complement(4759..4779)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 21.63"
                     /tool="antismash"
     misc_feature    complement(4794..4810)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 19.15"
                     /tool="antismash"
     misc_feature    complement(4795..4811)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.67"
                     /tool="antismash"
     misc_feature    4817..4832
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.11"
                     /tool="antismash"
     misc_feature    4819..4833
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.27"
                     /tool="antismash"
     misc_feature    complement(4881..4901)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.95"
                     /tool="antismash"
     gene            5001..5330
                     /locus_tag="B042_RS24285"
     CDS             5001..5330
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /locus_tag="B042_RS24285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_051415519.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 4.6e-11,
                     bitscore: 36.4, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSATFKRILSALLLTAIAIGATTTAAAALTTRYVGGGTWRYGTES
                     NYAHSRYYHPTECHSATVQRTVRQVGTVVAADRDVRGPKSWARAGLLNTGDYLEQAYWN
                     DEASC"
     gene            5414..7465
                     /locus_tag="B042_RS0107095"
     CDS             5414..7465
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B042_RS0107095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_028647699.1"
                     /transl_table=11
                     /translation="MHRIIVFAHATAITMALLLGFLVVFAMHQRFDTPPMGSHYVRIEH
                     DSNSVSWVEVSQALEETARENSLSIAAVEIDRDDINGRRTYYATSFDPSHPLHTWLLEG
                     YPGFSAQPLIEVRPWQEWQTEDLRTGFAGMGSPQEAEHVQQALRELGLETAAGRAPAPL
                     PWTDHLLRGETALTAAVTAVLVATSAGAGVLLNARSYAVARLAGHSYPRILGKDLAAIG
                     RWWALLLPCLTACAALALLLYNGLAQYQRLAAVSAWILAALALVALAAHALALGLLHRS
                     PLTTALKGHMPIRGVAAAVYTVRLSALVLAVAAATATVTALERVHAHTVGADDITRVQG
                     AARLYIGGTGDYGALVETDRDLLGPWLRRADAQGRTVLVLQEPLETLLPEGRHSARSAV
                     LVVNETYLEREGVLADVDGRGGVPRVFTPSPSTHTARELAEGATAWVGFRSPDPAPERV
                     PVIGYPPGRTFFTYGTYDPLTANLRPTVEDPVVLAVPNGLLEEEWYAISARSGGLLFLD
                     RRVAEHAVAERGLSFHVTAVESVSTSMATANRQATTELRINAVTLAVAAAVLVMTSTAA
                     CLVYARARSQHVFARHISGWSFAATHRLMLSVEAALASVLVGWAAADLAATLHAFADPA
                     GAVFGFDVLGAYRALAVSCSIAAAMAVIVTVVLAHFHRRIVREGAAQA"
     gene            7478..8113
                     /locus_tag="B042_RS0107100"
     CDS             7478..8113
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546332.1"
                     /locus_tag="B042_RS0107100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_028647700.1"
                     /transl_table=11
                     /translation="MITVRDLSKSFGARTLWRGLDLDLPPGRMLALTGPSGAGKTTLLN
                     CLGLLEAPTGGSISHGGRDLTRLRAGGRRRFRRDHLGYLFQNYALMENATVRENLDVAR
                     RDRPAQAEALERVGLAGRSRERVHHLSGGEQQRVALARLLVKQPSLVLADEPTGALDEG
                     NGQMVVDTLRAMAAEGCSVVIATHNSAVREACDTVLDVSTRQPATERA"
ORIGIN
        1 agagccacgc ccgcgccgtg ctgcccgatc cctgccccga ggccgacccc cgcggccccc
       61 tgttcggcag ggtcgtgtgc gtgtccgggg acctggccgc catggacaag ccacaggtgt
      121 ggcggcgcgt ggccgaagcg ggcgggcggc ccgccaagaa cgtcacgagg acgaccgacg
      181 tgctcgtggt gggagcccac agcggcccgg gaaagacggc caagcacctg cgggcgcagg
      241 cctaccgcga gcgcgggcag cgcatcgaca tcatcaccga ggccgaactg ctggtccgcc
      301 tgggcctggg ccgcggctga gcgccggagc cgttcacccc tgctccggcg cggtggacaa
      361 ctcggtcagg tgccgcgtca ggccctgctc ggcccaggtg cgccagcgcc gcacgctcca
      421 gccgcagtgg cgggtgtaga ggatgaacac ctccgggctg agcagggcca ggcaggtgtc
      481 ggcggcctcg tcggccgaca gctccggccg gagcgccccg cgttcgcgga gccgttcggt
      541 gaaggtgcgc tgcacggtcc ggcgctggtc gacgctggcg tgccacagcg cgcgcagctc
      601 gtcgtcggag tcggccgcgc cccgcaggac ctccgacagg ggagagaggc gctccatcac
      661 cgaggcggtg ccgacggtgt gcagccggat ctgaccgcgg gcgtcgggcg cctccagggc
      721 ccggcgcacc cagggccgat ccagcgtcgc cacgggtgcg gcgtcccccg ccacggcgtg
      781 gtccaaggcg tgcttgagga ccgccgactt ggtaccgaag gcgtagtaga cggtctgggc
      841 ggccaccccg gcctcggccg cgatgctctg gatgctggtg gcggcgtagc cgcgttcggt
      901 gaacagccgt gcggcggcgg tggccacccg ggcgcgggtg gcccgggacc gggcggtgcg
      961 tccgtcgacg gtgtgtgcgt ccatgcccgc atcctacggc ttgactggag tggcactaca
     1021 gataccttgg tggagttcta ctgcaaaaac tttcaagggt cgtcgtgcgc ggctccgcgc
     1081 ggggaggagc gggagggaag ccccgctccc ggacggttgc tacggggcgg ggggagcacc
     1141 ggtcgccgag cggctgtgcg cgttcggccg cacccgcccg gaaccgccag gcgctgggcg
     1201 ggtgcgggcg ggctcaggac gcggcggcgc gccgggcacg gacagcggcc cgggcgtcct
     1261 gggcgaccag gtcggcgttg atcgccgcgg cggcggccac cccggccccg gtcgagacgg
     1321 agaccatcgc cgacaggtcg gcggcgttgc ccgctgccca gaccccctcc acgtccgtgc
     1381 ggcccatcgg acccgtggcg atgaacgagc ccatcgggtg gtcggccgct gctccgccga
     1441 gctgttcgta caggtccgtg cgggcggtga agcggggggc gacggccacc gcgtccacgg
     1501 ggaacgcgtg cccctcggcc aagacgacgg ctcggaggcg cccgccctcg gtgctcagca
     1561 tccgcacggg gccctccacg acctggacgt ccagcgccgc catctgctcc cacgcctggt
     1621 cctcggggct gcccatggtg tgggtgaaga gcgtgacgtg ttcgctgagc tggcggaaca
     1681 tcagcgcctg gtggaggctg agctcactgg tgcccaagac accgatgcgc cggccgcgca
     1741 cctcccagcc gtggcagtag gggcagtgca gtacgtcggt gccccacagg tcccggaccc
     1801 cggggacgtc gggcagttcg tcgaccaggc ccgtggccag caggatccgc cgtgcccgca
     1861 gggttcctcc gtcgtccagg tccacctcga aggcgccctc gcagtgccgg gcggccacgg
     1921 cgcggccgtc gtggaagcgc gctccgtagc ggtccgcctc cgcgcggccg gccgtgagca
     1981 ggtcgcgcgg ggcgatcccc tcatggccca ggacgttgtg tgctcccgct gcgggtgcgt
     2041 tgcgcggctc gccgccgtcc acgacgacca ccgatcgcag ggaccgtgcc agtgtgacgg
     2101 ccgcgctcag cccggccgct ccgcctccga tgaccgcgac gtccaccccg tgacgtgtac
     2161 tcaccgtgca tcaccttcct cgtgggttcg gcccgcacgc tagccgcgga ccctcccccg
     2221 cgcacggatt cttgcccata cggcaacagt gttgccgggg cggcgcaccg gggcgatgtt
     2281 tctcaggagg ggtcggcgaa gcgcaggatg gcgccgctgt cggtggtggg ctcgccgtcc
     2341 tcgagcagtt cggtgaagtc ggagtcggtc atgaccgccg agcgcagcat gagcgcgctg
     2401 gccggggcgc ggaaggcggc ctcggggcgg tccaagtcgg tgcgctgggc agcgaccagg
     2461 acgggctcgg tacgtgagcc ccacaagtgg ggctcggcgt cgcgttcggc ccccatgagc
     2521 agggtgcgca cgcgagcctc cgagagcatg ggcagggtcg gctcggttcc ggccacggcc
     2581 tgctcgtggg cgagcttgct ccggaagtcg gcggccacgc gctcctggtc gctggtgacg
     2641 aactggtgca gggcctcagc ggcggcctgg tgcaggcgtt cctcggcgtc ggttccaccg
     2701 cgtccgccgg gcaggaccac gatcggcagt tcggtcctgc gctcgccgag gttgtcgcgc
     2761 aggtaggcga tggcctcgtc gtcgccgccg acgaacacga cgtcggcggc gacgtcggcg
     2821 acggcctcgc ggacgtggtg ggcgaccttg gcggtgttct cgcgccacag ttcctgggcg
     2881 acgtgcttgc cggggaagcg tccgttgtag ccgccgaggt tgccggggcc gccgcggctg
     2941 gtgacgtcca ggccccgcag ggaggcaccg gtcaggtcct gctcggaggc ggggccttcg
     3001 gcggggtagg cggggtaggc ggtgatgtgg gcgttgacgc ggtccagcgc caccacgacg
     3061 tagggcaggt ggcggccgcg gtcgatggcc acgggcaggg cgtcgggcac cggcagccag
     3121 gtagcgcggt cctggtccgg gggctcggtg agcgtgtagg cggagatgag ttcgccctgg
     3181 gaggcgaaca gggcctgtcc gtgcgtgccg aaggagcggg agttgtgctc cacgatggct
     3241 gtctccaggg cgttgagcgt ggcctggtcg gcaccgagtg cgtccagtgt ggcgcgcagg
     3301 tggcgccagc gcagttcgat cttcttgtcg gcgtccctgg actcgcggct ggtgtccagg
     3361 tgcaccgagg cgatcggggc gtcgctttcg tagagaggtc gcagaaagcc aaggtccatg
     3421 atccgcggag cggtgtgccc ccgctccgcc cacctccttc tcaagggtcg gcctctgggc
     3481 catggtggtt cccatggtgg ccccctgtca tggccgccgc gccgaacaag aggggcttct
     3541 tgctgcgata tgtccttgtt ttgcttggtg tgagtgttgt gggagtcagc gttccgagga
     3601 acaggtgcgg tcgcgcagga agcggtgcac cgcccggttg aaggcctggg gccgttccag
     3661 gttgggcagg tgggcggcgc cctcgatgat ctccacgacc gagtcgggca ccagcacgtg
     3721 catcgcctcg gtgagcccgg gggaagtgag ggtgtcgtgc tgtccggcca cgagcagggt
     3781 cgggcaggac acccgtttga gcaggggcac gtagtcggga cgcagggcgc ggcccagcag
     3841 ggccgcggcg gccccctcgg ggtcggtgcc gcgcatcatg gtgtgcacgt gccgggccgt
     3901 gtcggggctg cggcggacgt tgtcggcgct catcatgccc acgagcatcc ggtcggcgta
     3961 gcctgccatg ccctcggccc tgagcagggc ggccaggcgg cggcgctcca ggcggcggcg
     4021 ctcggcctcg gggtgggggt cggtggcggc caggaccagg gagtcgacgc ggtcggggaa
     4081 gagccggtac agctccaggg cgacctggcc gcccatggac aggcccacca cgcccacggt
     4141 gtccgcgccc gcgtggtcga gcagggcggc caggtcgcgg gcgaagacct ccagcgtggt
     4201 ggttccggga acgacggtgc tcgtgccgta gccgcgcagg tcgggggcga tgacccggta
     4261 tccgtgtgcg gacatgaact ccgtctgggg ggtccacatg gtgcggtcga aggggtggcc
     4321 gtggatcatc agcagggggt atccggtgcc gcggtcgctg tagccgacgt tgattccgcg
     4381 tacgggtgcg gtgttcgggc gatcgctgcc cgcggcgttc gtcgtcagag gtgccacggc
     4441 acacgatgct attgcctgtc gcacgaactg tctcggttcg gtcgataccg ggcggagtgt
     4501 gcggggccgc aggcggcccc tcgtcgcctg cggccccggt acgaggtaga gacgcacgga
     4561 agcgcgtccg ggttcggttc ccggcgggat tggcttccgg cccccggtcg gcgctgcgga
     4621 ctttcggccg cgtgccgtcg gccgccccgc ggcgggggac cgaggtcgtt cgcgtccggc
     4681 tccaggccgt ggttcggggc ggtggccggc cgcgccgaga ggagcggggt tcggggacga
     4741 tggttttccg tttttcggtg atccgcatgc cgacgaaaaa tccggattga ccgattttat
     4801 ctaatttcca agggaatatg aaataccttt tcttgtccac agaggcgacc tggccattgc
     4861 cgatcccgtg cgggcactgt tagttttctt tgtgccgaga aggaacgagg aaagtcgacc
     4921 gccgccgcgg attcccgcgg aaggagtgcc ggccgtcccc ctcaattccg gacagtcctt
     4981 tgaacgcgaa cggagcaaca gtgtccgcca ccttcaagcg catcctctcc gccctcctgc
     5041 tcaccgccat cgccatcggc gccacgacga ccgcggccgc cgcactgacc acccgctacg
     5101 tgggcggcgg cacctggcgc tacggaaccg agagcaacta cgcccactcc cgctactacc
     5161 accccaccga gtgccactcg gcgaccgtcc agcgcaccgt caggcaggtc ggaacggtgg
     5221 tggccgccga ccgggacgta cgcggcccca agtcgtgggc cagggccggc ctgctcaaca
     5281 ccggcgacta cctcgaacag gcctactgga acgacgaggc ctcgtgctga ggcccacgcc
     5341 tcccgcgagg tccgcctgaa cacagccagg agggccgtgc ccggcagggc cctccgccac
     5401 cttctccggg accatgcaca ggatcatcgt cttcgcccac gccaccgcca tcaccatggc
     5461 gctcctgctg gggttcctcg tggtgttcgc catgcaccag aggttcgaca ccccacccat
     5521 gggaagccat tacgtcagaa tcgaacacga cagcaattcc gtgagttggg tcgaggtatc
     5581 ccaagcgctg gaggaaaccg ccagggaaaa cagcctttcg atcgcggcgg tggagatcga
     5641 cagagacgac atcaacgggc gccgcaccta ttacgccacc tcgttcgatc cctcccaccc
     5701 actacacaca tggctgctcg aaggctaccc cggattcagc gcgcaacccc tcatcgaggt
     5761 ccgcccctgg caggaatggc agaccgagga cctgcgtacc ggtttcgccg gaatgggctc
     5821 gccccaggag gccgaacacg tacaacaggc actcagggag ctgggcctgg agaccgccgc
     5881 cggcagggca cccgccccgc tcccgtggac agaccacctg ttgcggggcg agaccgcact
     5941 gacggccgcg gtcaccgcgg ttctggtggc cacctcggcc ggcgccggag tcctgctcaa
     6001 cgcccgctcc tacgcggtcg cacgcctggc gggccactcc tacccgcgga tcctcggcaa
     6061 ggacctggcg gcgatcggcc ggtggtgggc cctgctgctg ccctgcctca ccgcctgcgc
     6121 ggctctcgcc ctgctgctct acaacgggct cgcccagtac cagcgcctgg ccgcggtgtc
     6181 agcgtggatc ctggcggcgc tggccctggt ggccctggcc gctcacgctc tggccctggg
     6241 actcctgcac cggagcccgc tgaccaccgc actcaaagga cacatgccga tccgcggcgt
     6301 ggccgccgcc gtgtacaccg tgcgcctgag cgcgctggtc ctggccgtgg ccgcggccac
     6361 ggccaccgtc accgccctgg agagggtgca cgcccatacc gtgggcgccg acgacatcac
     6421 ccgcgtccag ggagcggccc ggctctacat cggcggcacc ggcgactacg gagcactcgt
     6481 ggagaccgac cgcgacctcc tcggcccctg gctgcgccgg gccgacgcac agggccgcac
     6541 cgtcctagtg ctccaggagc ccctggagac cctcctcccc gaaggccggc acagcgcccg
     6601 ctcggcggtg ctggtcgtca acgagaccta cctggaacgc gaaggcgtcc tcgccgacgt
     6661 cgacggccgc ggcggggtac cgagggtgtt caccccctcc cccagcacac acaccgcacg
     6721 ggaactggcc gagggggcca ccgcctgggt cgggttccgt tccccggacc cggcgccgga
     6781 acgggttccc gtcatcggct acccgccggg ccgcaccttc ttcacctacg gcacctacga
     6841 ccccctgacc gcgaacctgc gcccgaccgt ggaggatccg gtcgtcctgg ccgtccccaa
     6901 cggcctgctg gaggaggagt ggtacgccat cagcgccagg agcgggggcc tgctgttcct
     6961 ggaccgccgc gtcgccgaac acgccgtcgc cgaacgcggc ctgtcgttcc acgtcaccgc
     7021 cgtcgaatcc gtctccacct cgatggccac cgccaaccgg caggccacca cggaactgcg
     7081 catcaacgcg gtcaccctgg ccgtggccgc agccgtcctc gtcatgacca gcaccgccgc
     7141 gtgcctggtc tacgcgcgtg cgcgctccca gcacgtcttc gcccgccaca tcagcggatg
     7201 gtcgttcgcg gccacgcacc ggctgatgct ctcggtggag gcggcgctgg cgagcgtgct
     7261 cgtcggctgg gcggccgcgg acctggccgc caccctgcac gcgttcgccg accccgccgg
     7321 agcggtgttc gggttcgacg tgctgggggc ctacagggcc ctggcggtct cctgtagcat
     7381 cgctgcggcc atggccgtca tcgtcaccgt cgtactcgca cacttccacc gccgtatcgt
     7441 ccgtgagggc gccgcccagg cctgaaaggg atctgccgtg atcaccgtcc gcgacctgtc
     7501 caagtccttc ggcgcccgca ccctgtggcg gggcctggac ctcgacctgc cccccgggcg
     7561 gatgctcgcc ctgaccggcc cctccggggc gggcaagacc accctgctca actgcctggg
     7621 cctgctggag gcccccaccg gcggcagcat cagccacggc ggacgcgacc tcacccgcct
     7681 gcgggccggc gggcggcgcc gcttccgccg cgaccacctg ggctacctct tccagaacta
     7741 cgcgctgatg gagaacgcca cggtgcgcga gaacctggac gtggcccgcc gcgaccggcc
     7801 cgcccaggcc gaggccctgg agcgggtggg gctggccggc cgctcccgtg agcgggtgca
     7861 ccacctctcg ggcggcgaac agcagcgggt ggccctggcc cgcctgctgg tcaagcaacc
     7921 gtccctggtg ctggccgacg agcccaccgg cgccctggac gagggcaacg gccagatggt
     7981 ggtcgacacc ctgcgcgcca tggccgccga agggtgttcg gtggtcatcg ccacccacaa
     8041 cagcgccgtg cgcgaggcct gcgacaccgt cctcgacgtc tccacccggc aaccggccac
     8101 cgaacgggcc tgaccccggg cgggaccctg cccgg
//
