LOCUS       NC_022873              10249 bp    DNA     circular CON 15-AUG-2024
DEFINITION  Bacillus thuringiensis YBT-1518, complete sequence.
ACCESSION   NC_022873
VERSION     NC_022873.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis YBT-1518
  ORGANISM  Bacillus thuringiensis YBT-1518
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 6002284)
  AUTHORS   Wang,P., Zhang,C., Guo,M., Guo,S., Zhu,Y., Zheng,J., Zhu,L.,
            Ruan,L., Peng,D. and Sun,M.
  TITLE     Complete genome sequence of Bacillus thuringiensis YBT-1518, a
            typical strain with high toxicity to nematode
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6002284)
  AUTHORS   Wang,P., Zhang,C., Guo,M., Guo,S., Zhu,Y., Zheng,J., Zhu,L.,
            Ruan,L., Peng,D. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2013) State Key Laboratory of Agricultural
            Microbiology, College of Life Science and Technology, Huazhong
            Agricultural University, 1 Shizishan Road, Wuhan, Hubei 430070, P.
            R. China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 1.04
            Reference-guided Assembly         :: NC_005957.1
            Genome Coverage                   :: 190X
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000497525.1-RS_2024_08_15
            Annotation Date                   :: 08/15/2024 02:37:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,968
            CDSs (total)                      :: 6,823
            Genes (coding)                    :: 6,362
            CDSs (with protein)               :: 6,362
            Genes (RNA)                       :: 145
            rRNAs                             :: 15, 15, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 15, 15 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 461
            CDSs (without protein)            :: 461
            Pseudo Genes (ambiguous residues) :: 0 of 461
            Pseudo Genes (frameshifted)       :: 191 of 461
            Pseudo Genes (incomplete)         :: 266 of 461
            Pseudo Genes (internal stop)      :: 128 of 461
            Pseudo Genes (multiple problems)  :: 100 of 461
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:11:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2936960
            Orig. end                         :: 2947209
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP005935.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10249
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10249
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10249
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5249](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5249
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            979..2339
                     /locus_tag="YBT1518_RS14740"
                     /pseudo=""
     CDS             979..2339
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017763222.1"
                     /locus_tag="YBT1518_RS14740"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="glycosyl hydrolase family 8"
                     /pseudo=""
                     /transl_table=11
                     /translation="MNGKRKIFTCISIVGIGLASFSNSSFAASVTENSIQNSIPVVNQQ
                     VAAAKEMKPFPQQVNYAGVIKPNHVTQESLNASVRSYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMIITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADSKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGTVNYLKEAQDMITKGIKAS
                     NVTNNNRLNLGDWDSKSSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFSN
                     KYSPNTGLISDFVVKNPPQPAPKDFLDESEYTNAYYYNASRVPLRIVWTMRCTARSEVK
                     SFLIKSLHGFKIKRMEILLKLWMVIN"
     gene            2502..3425
                     /locus_tag="YBT1518_RS14745"
                     /old_locus_tag="YBT1518_14800"
     CDS             2502..3425
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="YBT1518_RS14745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14800"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_023522096.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATKPLTEEITTYYPEIHGAEGLGPIHVPENILSIPIYNF
                     GSIAAILIKYGKDLTIVDVGRSTSLAIAFNLWNDLMLNVKGIYFMGGVFLEVGNVTPLA
                     EANVYGDPIASKIVFQQAKNLFIFPLNVTNKAVLTPNVFNYIQANTKNPFHTIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVVAISGLVNPSFFEFVHRTVNVDTYGDTKGQTFADFRPN
                     SISEGARIALDIDAEKFIQDFIKIML"
     gene            3502..3960
                     /locus_tag="YBT1518_RS14750"
                     /old_locus_tag="YBT1518_14805"
     CDS             3502..3960
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="YBT1518_RS14750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14805"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_001102684.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEEALKLQVKGNQLKFVPSVAVSLAKVYIKPDG
                     DNVEYIPFAIYDGDLMVGFVMHAVVIETSDMYWINGFIIDQSQQGNGYGKAALQESIDI
                     IKNTFKSCKEIRLTVHKDNISAKKLYERYGFISLGQEYDGEQVYRLFV"
     gene            3991..4329
                     /gene="cutA"
                     /locus_tag="YBT1518_RS14755"
                     /old_locus_tag="YBT1518_14810"
     CDS             3991..4329
                     /GO_process="GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="cutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160602.1"
                     /locus_tag="YBT1518_RS14755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14810"
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="WP_023522097.1"
                     /transl_table=11
                     /translation="MQFTEVKIEVFIPEEYIETLRDELNKIGACKTGEYDHCLSYSSVK
                     GYWRPLEEASPFNGEIGRICEGQECKVEIKCKRELVKSALEVINAIHPYETPMIYIIPI
                     LNDYFEQV"
     gene            4355..4801
                     /locus_tag="YBT1518_RS14760"
                     /old_locus_tag="YBT1518_14815"
     CDS             4355..4801
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="YBT1518_RS14760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14815"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_023522098.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAVVIKDEKVLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELKEELGVNCSIKRFLGVIENQWQDKEVLHHEINHIFEVESQGLHTDLTPHSSESH
                     LAFHWIDYSKESLTTHKIMPTPSVKELLERKLSDELLNCWISNF"
     gene            5001..5249
                     /locus_tag="YBT1518_RS14765"
                     /old_locus_tag="YBT1518_14820"
     CDS             5001..5249
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071345.1"
                     /locus_tag="YBT1518_RS14765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14820"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001071345.1"
                     /sec_met_domain="TIGR03601 (E-value: 5.8e-43, bitscore:
                     143.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQHELQTLNLNDYQTGNVVYWDQQQSQYPYYYIQGDARRCGG
                     CGGCGGRCGGCGGGRCGGCVGCAGCFGCFNCWNWWII"
     gene            5351..6367
                     /locus_tag="YBT1518_RS14770"
                     /old_locus_tag="YBT1518_14825"
     CDS             5351..6367
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288913.1"
                     /locus_tag="YBT1518_RS14770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14825"
                     /product="serine hydrolase"
                     /protein_id="WP_023522099.1"
                     /transl_table=11
                     /translation="MYTYEKLISWVENIKEKNHSSATALCIIKDNKIVLEHYNGYHSNT
                     FNSKEVTASSQFNVASARKSYLGLMIAYALYEGKINSIDDEAIKYFKDFDPVLLGKTTL
                     RHLVTHSHGLGETNDGTIFREFEPGQAWAYRDINVRMMTRLIYQLYSKSFPELLKERVF
                     KPANFQETGWRIQQDENLVKVINNPNEDAISEIGTVDDGTEKNLFVSAREFAQWGNLHL
                     NQGKIHDKQIVPQEVIKIATSLQSPTYINKDLPQNGLFWFVQNKPSRLSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRFNYGGDNYLYYLREFSNLVADTFSSSNRE"
     gene            6399..6542
                     /locus_tag="YBT1518_RS37565"
                     /old_locus_tag="YBT1518_14830"
     CDS             6399..6542
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001997600.1"
                     /locus_tag="YBT1518_RS37565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14830"
                     /product="hypothetical protein"
                     /protein_id="WP_023522100.1"
                     /transl_table=11
                     /translation="MKENGKKNKKQNIKDVNTEQNAIFSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            6827..8035
                     /locus_tag="YBT1518_RS14780"
                     /old_locus_tag="YBT1518_14835"
     CDS             6827..8035
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="YBT1518_RS14780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14835"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_023522101.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQKKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDNLGVS
                     QAFLYWGIIVLVLVLIGSFFLREAIVSNAVTETLHNDYTPREMIRTKQVYLLFFMLFTS
                     CMGGLYLISMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIILGTLSDKIGRMKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGAILGGFQPTFIIIGVLSVISFIISILIRPPNVEKKKELKHLHR
                     KVA"
     gene            8166..8591
                     /locus_tag="YBT1518_RS14785"
                     /old_locus_tag="YBT1518_14840"
     CDS             8166..8591
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706722.1"
                     /locus_tag="YBT1518_RS14785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14840"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_000706912.1"
                     /transl_table=11
                     /translation="MKIVQQWVQEDSDYIREKVIEYNQKYLAGEEKTPSEKVSFIVRNE
                     KEEIVGGVTAITFWHHIHVDFLWVSEEYRHEGYGSKLIKLIEEFAIEKECRLINLDTFS
                     FQAPDFYKKHGYKVIGVSEDHPKGHNHYYLEKRLKSI"
     misc_feature    complement(8772..8790)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.4"
                     /tool="antismash"
     gene            8871..9314
                     /locus_tag="YBT1518_RS14790"
                     /old_locus_tag="YBT1518_14845"
     CDS             8871..9314
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="YBT1518_RS14790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14845"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_000757628.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDKTNEKILMVKNKGENGSYYTLPGGAVKLGETL
                     EEAVIREVKEETGLHINVNGICSISEAFFEERDHHAIFFNFLGEIIGGETYISRPKEIE
                     EITWMELHIAAPYLRIPEHLLDLLQKKETVPYFFNGTIVHQSS"
     misc_feature    10101..10119
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.64"
                     /tool="antismash"
ORIGIN
        1 ccagcaattc gtatggggct tgaatcttat acgcctgaac accttacttt actacgctta
       61 ttaatcgctt catttatact tttgttattt tcatttatat acaaactacg attaccggat
      121 ttaaaggata tcccagcaat ttttatattt ggtgctttag ggtttactat ttatcacatc
      181 gcattaaact acggcgaaaa aacagtaaat gctggatctg caagtttaat aatttcagtc
      241 actccaatag tgacggcaat tcttgcttct gtttttttga atgacaaaat gaaattaaat
      301 ggttggcttg gtggtgtaat tagttttgca ggaattgcct taatttcatt tagccagggc
      361 gatgctattc aattgaatag cgggggatta tttatattat tagcggcgat ttcagaaagt
      421 ctatttttcg ttttccaaac atcttactta aaaaaatacg gcttcttacc atttaccata
      481 tatacaattt tgtctagtac tgtatgtatg cttattttct tacccggagt ttatcaagaa
      541 gtactagcag ctcctcttga agttaacttg agcgttatat atttaggtgt ctttccaaca
      601 gtactcccgt atattgcatt ggcctatatt atatctcgtg ctggtgcttc tgaagcaaca
      661 agttctctat atttaacgcc aataactgcg tgtttcattg cttggatatg gttaggagaa
      721 gtgccaactt tagtttcgat aattggcgga ggaattacta tacttggaat tgtgattgct
      781 catataccag tattaagaaa agaaaaacat aggaatttag caaataatca atccgtataa
      841 tgtaattcaa taataatttt atgatacttt tttatatagt ttctttctta tgttgataaa
      901 tataatattt tgtatttcag ttaaatattg tatgttacat tttatgtagt aagcatttta
      961 aaaggagctg acaacataat gaatggaaaa agaaaaattt tcacatgtat ttctattgta
     1021 ggaatcggac tagctagttt ttctaattct agtttcgcag caagtgtaac ggaaaattca
     1081 atacaaaatt ctattcccgt agttaatcaa caagtagctg ctgcaaagga gatgaaacca
     1141 tttccccagc aagttaatta tgcaggtgtt ataaaaccga atcatgttac acaggaaagt
     1201 ttaaatgctt ctgtaagaag ttactatgat aattggaaaa agaaatattt gaaaaatgat
     1261 ttatcttctt tacctggtgg ttattatgta aaaggagaga ttacaggtga tgctgatggg
     1321 tttaagccac ttggaacttc agaaggtcaa gggtatggga tgataattac agtattaatg
     1381 gctggttatg attcgaatgc tcaaaaaatc tatgacggtt tatttaaaac agcaagaact
     1441 tttaaaagct ctcaaaatcc taatttaatg ggatgggttg tcgcagatag taaaaaagca
     1501 caaggtcatt ttgattctgc tactgatggg gatttagata ttgcgtattc tcttcttctt
     1561 gctcataagc agtggggatc taatggaaca gttaattatt tgaaagaagc acaagacatg
     1621 attacaaaag gtattaaagc tagtaatgtt acaaataata accgactaaa tttaggagat
     1681 tgggattcta aaagttcact tgatacgaga ccatctgatt ggatgatgtc acaccttaga
     1741 gcattttatg aatttacagg ggataaaact tggcttactg ttattaataa tttgtacgat
     1801 gtttatacgc aatttagtaa taagtactct ccaaatacag gacttatttc agatttcgtt
     1861 gtaaaaaacc caccacaacc cgcacctaaa gacttcttag atgagtcaga atatacaaat
     1921 gcatattatt ataatgctag tcgagtacct ttaagaattg tatggactat gcgatgtacg
     1981 gcgagaagcg aagtaaaatc atttctgata aagtctcttc atggattcaa aataaaacga
     2041 atggaaatcc ttctaaaatt gtggatggtt atcaattaaa tggatccaat attggtagtt
     2101 atccaactgc tgtatttgtt tcaccgttta ttgctgcaag tataacgagt agcaataatc
     2161 aaaagtgggt aaatagcggt tgggattgga tgaagaataa gaaagaaagt tattttagtg
     2221 atagttataa tttattaact atgttattta ttacaggaaa ttggtggaaa cctgtacctg
     2281 atgataaaaa aatacaaaat caaataaatg atgcaattta tgaaggatac gataattaat
     2341 taaatttgag aatggttact gaagaaagga attagcattc gctagttcct ttttaatatt
     2401 ttgtaaaaca ttatccattc aaaaattcgc atagatatac ttgctttttt catatacata
     2461 gttattgatg actctatatt tttgtgaggg gagagtattt cttggaaaaa gtattatttt
     2521 tcggagaccc aggtattgat gactcatttg ccattatgta tggcttactg catcccgaaa
     2581 ttgaaattgt cggtattgta actggatacg gaaatgttga gcatatacat gctgcacata
     2641 atgcagcgta tattttgcag ttagcaaata gacaagacat tcctgtcatt agtggtgcaa
     2701 caaaaccact tacagaagaa attactacgt actaccctga aattcacgga gcagaaggat
     2761 taggacccat ccatgttcca gaaaatatat tatctattcc aatatataat ttcggcagta
     2821 ttgctgcgat tcttataaag tacggaaaag atttaacgat tgttgatgta ggaagatcta
     2881 cctcgttagc aattgctttt aatttatgga atgatttaat gctaaatgta aaaggaatct
     2941 atttcatggg tggtgttttt ttagaagtag gaaacgttac gccattagca gaggcaaacg
     3001 tgtatggaga tccgatagca agtaaaattg tttttcagca agcaaagaat ttattcatat
     3061 tccctttaaa tgtaacaaat aaagctgtgt taacaccgaa tgtatttaac tatattcaag
     3121 ctaatacaaa gaatccattt catacgataa tgaaaccgat gtatgattac tatttatctg
     3181 cataccaaaa acttaatcca tctatcgaag ggcctttgct acatgatgtg gttgcaataa
     3241 gtggattagt aaatccaagt ttttttgaat ttgtgcatcg tacagtaaat gtagatacat
     3301 acggagatac gaaaggacaa acatttgctg attttcgccc taactctata tctgaaggag
     3361 cccgtatagc tttagatata gatgcagaaa aattcattca agattttata aaaattatgt
     3421 tataagttgc tgtcgaaaaa gggaattatt tcaatacgtt gaatataaaa atacatcctc
     3481 tatataaagg agcttacaat attgaatgtc cagttaaagg ttgttacgag agaaaattgg
     3541 gaagaagcat taaagctaca agtaaaagga aatcagttga aatttgttcc atccgtagca
     3601 gtttcgcttg ctaaagtata tataaaacca gatggtgaca atgtagagta cataccattc
     3661 gctatatacg atggtgacct tatggttggt tttgttatgc atgctgttgt aatagagaca
     3721 tcggatatgt attggattaa cggatttatt attgatcaat cgcagcaagg taatggttat
     3781 ggaaaagcag cattacaaga aagtattgat ataataaaaa atacttttaa atcgtgtaaa
     3841 gaaataagat taaccgtaca taaagataat atctctgcaa agaaattata tgaacgatat
     3901 ggtttcatat cattaggaca ggagtatgat ggtgagcaag tatatcgttt atttgtttaa
     3961 ctattaggaa gtaaaggtgt gaaatgaaat atgcaattta ctgaagtgaa aattgaagtg
     4021 ttcattcctg aagaatatat tgaaacatta agggatgaat taaataaaat tggagcatgt
     4081 aaaacgggcg aatatgacca ttgtctttcc tatagttcag tgaaaggata ttggagacct
     4141 ttagaagagg catctccttt taatggagaa attgggcgaa tatgcgaagg gcaagaatgt
     4201 aaagttgaaa taaagtgtaa acgagaactt gtaaaaagtg ctcttgaagt tattaatgca
     4261 attcatccat atgaaacacc aatgatttat ataatcccga tattaaatga ttattttgaa
     4321 caagtataga ttgaatgact aggaggaata tcaattgaaa agtaaattcc atcatattgt
     4381 acgagcagtc gtgataaaag atgaaaaagt attagtcgct gaatatattg gccatcatta
     4441 tttcttgcca gggggtcatg ttgaaattgg tgaatcagca gagaatgcat taatacgaga
     4501 actaaaagaa gaacttggag taaactgcag tataaaacga tttttaggag ttatagaaaa
     4561 tcaatggcaa gataaagaag tgcttcatca tgaaattaat cacatttttg aagtagagtc
     4621 acaaggttta catacagatt taacaccaca ctctagcgaa tctcatttag catttcattg
     4681 gatagattat agtaaagaat ctttaactac tcataaaatc atgccaacgc cttcagttaa
     4741 agaattacta gaaagaaaat taagtgatga actactaaat tgttggataa gtaattttta
     4801 aatcactcta tttatagtaa atacgtccat cgaaaggata taaagaaaat aggctaataa
     4861 acaaggtaca tcacctagtg taagttcata ttttgttgat gtacgcattt agtctatagt
     4921 cttgtttcta ttttgtttac aaaaatatat aaactttaac gtttgggcgg aatttatatt
     4981 tataaatgga ggggttcgta atgaatcagt ttcaacatga attacaaaca ttaaacctta
     5041 atgattatca aactggtaat gttgtgtatt gggatcaaca acaaagtcaa tatccatatt
     5101 attacatcca aggcgatgca cgtcgttgtg gcggatgcgg aggttgtggt ggacgctgtg
     5161 gcggttgcgg tggcggcaga tgtggtggtt gcgtaggttg tgccggatgt tttggttgct
     5221 ttaattgctg gaactggtgg attatttaga tatagtacat cattaatatt ttatatgaaa
     5281 gaaaggtgta aatactgttt atacttttct ttcatgctgt aattttgtat taggattaag
     5341 gaggatttat ttgtatacat acgaaaagtt gatttcttgg gtagagaata taaaagaaaa
     5401 aaatcatagt tctgctacag cactatgtat tataaaggat aacaaaattg tactagaaca
     5461 ttataatgga tatcactcaa atacatttaa tagtaaagag gtaacagcat cttcgcagtt
     5521 taatgttgct tctgcgagaa aaagttattt aggattaatg atagcgtatg ccctttacga
     5581 ggggaaaata aactctattg atgatgaagc gataaagtat tttaaagact tcgatcctgt
     5641 attacttggt aaaacaacgc taagacattt agtaacccat tcgcatggtt taggtgaaac
     5701 gaatgacgga acaatttttc gtgaatttga acctggacaa gcatgggcgt atagagatat
     5761 taatgtaaga atgatgacac gtcttattta tcagctatat agtaaaagtt ttcctgaatt
     5821 attaaaagag cgtgtgttta aacctgctaa ttttcaagaa acaggttgga gaattcagca
     5881 agatgaaaat ttggttaaag ttattaacaa tccaaatgaa gacgcgatta gtgaaattgg
     5941 tacagtagat gacggtactg aaaaaaatct gtttgtctca gctagagaat ttgcacagtg
     6001 gggcaatctg catttaaacc aaggcaagat acatgataaa caaattgttc cacaagaagt
     6061 tataaaaatt gctacaagtt tgcagagtcc aacatatata aacaaagatc taccgcaaaa
     6121 tgggttgttt tggttcgttc aaaataaacc ttcaagatta agtgaacttg gtgaacgagt
     6181 tccgaaaggg tcgtatcaaa tattagggat tactggaccg accattttag taatacctga
     6241 atataatgta gttgttgcaa aaatgtataa taaaagattt aactacggcg gtgataatta
     6301 tttatattat ttacgtgaat ttagcaattt agtcgctgat acatttagta gcagtaatag
     6361 ggaataatta ctcctataac aaaaaggagt gatctaaagt gaaagaaaac gggaaaaaaa
     6421 ataaaaaaca aaatataaaa gatgtaaaca cagaacaaaa tgcgattttt agcgatccta
     6481 aagatgcagc taatatgcaa acggtaccgc aaccgaagga ttttgatgaa attgaatact
     6541 aatttttcgt ttatccgact atatgatgaa tgaaaaatac atcttaagtc ccatcacatg
     6601 acagaaatct tttggtatcc tatatacata agcatttgaa aatgtcttat cttaacaaag
     6661 ctttctttag aaatatctga tataacctgc aaaaaggcac ttctttatga agtgtctttt
     6721 tgtgtatatg aacttaatta ccattttccg taccgtatct tgtatttttc aatggatata
     6781 ctaactgtga aaattcaagt caagataaaa gaaggggaca gttagtatga aacaaacttc
     6841 tataaatcct ctattaattg tgctaggtac aattattgtt caaattggcc ttggaacaat
     6901 ttatacatgg agtttattta atcaacccct tgtaagtaag tttggatgga accttaattc
     6961 agtagcgata actttctcca taacaagttt ttctttatca ttctcaacct tatttgcagc
     7021 aaagttgcag aaaaaattag gacttcggaa actcatcgct actgcaggaa ttgtattagg
     7081 actcggttta atacttagtt cacaagtttc ttccttacca ctactatatt tattagccgg
     7141 tgtcgttgtt ggttatgcgg atggaacagc ttatattaca tcactatcta atttaattaa
     7201 atggtttcca aatcggaaag gacttatttc aggtatatct gtatcagcat atggaatggg
     7261 cagcttaatc tttagatata taaacggaaa tcttatcgat aaccttggtg tatcacaagc
     7321 attcttatat tggggtatta tcgtgttagt tttagtatta attggatcat tcttcttgcg
     7381 tgaggcaatt gtaagtaatg ctgtaactga aacattacac aatgactata ctccacgtga
     7441 aatgatacga acgaaacaag tatatctgct attttttatg ttattcacct cgtgtatggg
     7501 tggtttgtat ttaatcagta tggtaaaaga tatcggcgta caactcgttg gacttagcgc
     7561 agcaaccgct gcaaacgccg ttgctatgat tgcaatcttt aatacagtag gtagaatcat
     7621 tcttgggacg ttatcagata aaatcgggcg aatgaaaatt gtctctgcaa cgtttattat
     7681 tataggtttg tcagtcttta ctttaagttt tattccgcta aattacggga tctattttgc
     7741 ttgtgtagca agtgtcgcct tttgcttcgg cggtaatata actatattcc cagctattgt
     7801 cggagatttc tttggattaa aaaaccatag tacaaattac gggattgtct accaaggttt
     7861 cggatttggt gcgcttgcag gatcatttat tggagcgata ctcgggggat ttcaaccaac
     7921 tttcattata atcggtgttt taagtgttat ttccttcatt atttcaatat taatccgtcc
     7981 accaaatgta gaaaagaaaa aggaactaaa acatttacat cggaaagtag cttaatttta
     8041 aaaagagtcc tcatactagg gctctttttt attccattaa ctttgcttct ttactaactc
     8101 attaaagaca ggacaatatc attttaaaat tgaatagata agtaaaaagg agcgtgacac
     8161 ttaaaatgaa aatagtacag cagtgggtac aagaagatag tgattacata agagagaaag
     8221 taattgaata caatcaaaaa tatcttgcag gtgaagaaaa aacaccttca gaaaaggtta
     8281 gtttcatagt aagaaatgaa aaagaagaaa tagtaggtgg ggtaacagca ataacttttt
     8341 ggcaccatat acacgttgac tttctatggg tttctgaaga atataggcat gaaggttacg
     8401 gaagcaagtt aataaagctt attgaagagt ttgcaattga aaaagagtgc aggttaatca
     8461 atttagatac ttttagtttt caggcacctg atttttataa gaagcatgga tataaagtaa
     8521 tcggagtaag tgaagatcat ccgaaaggac ataatcatta ttatttagaa aaacggctaa
     8581 aaagtatata gaatttgtta acattagtat ttggaataca catgcaataa gggcgaggtt
     8641 attattaatt tcagtattaa ttgaatcaat ttttattcta aaactgcaag aacgtacttt
     8701 ggataatctt cctaaggtac gttctttttt tatatcagta gcctattctt attaattcca
     8761 ccaatactgg taaaggcaag acttaactaa cttatatata ttaaaaggag ttaatggtaa
     8821 aatgaataga agacagaaaa ttccttttat taaaaataga ggaggtacat atgaaaaaag
     8881 taaatgttac atatgcactt ttatacgata aaacaaacga aaagatctta atggtaaaaa
     8941 acaaagggga aaatggttct tattatacat taccaggtgg cgcagttaaa ttaggagaaa
     9001 cgttagaaga agcagtaatt cgagaagtaa aagaagaaac agggttacat ataaatgtaa
     9061 acgggatttg ttctatcagc gaagcctttt ttgaggaaag agatcatcat gcaattttct
     9121 ttaattttct aggtgaaata attggcggag agacatatat atcaaggcca aaagaaattg
     9181 aagagattac ttggatggaa ttacatatag cagcaccgta tttacgcata ccagaacatt
     9241 tactagattt attacaaaag aaagaaacag taccttattt ttttaacgga acaatcgttc
     9301 atcaatcttc ataaaaatat tatctcatgt aaggcgggga aaacatggag tttatcgtta
     9361 atcacaaaca attcactcaa gcactttcag aagtaagtaa agcaatatca acaaaatctt
     9421 taattcctat attatctggt ataaaaataa ctgcagatca atctggaatt actttaatcg
     9481 caagtaactc gaatattttt atcgaaaaat tcattcctgt ttcaattgaa gacgaaaaga
     9541 ttgcaactat tttaaaggca ggaactattg ttgtacctgc aaagtatttc attgaaataa
     9601 taaagaaaat gccaagtgat atagcaataa aaagtaagaa tgagcaatta attacgatac
     9661 aatcagaaga aatcacatta agtttaaatg gatttcctgc agatgagttt ccgaatgtac
     9721 cgcttataga caatcatgca gaaatcaaag tagagacaga gcaattgatt gaagtattta
     9781 aacaaacagt ctttgcggta gcgaaaaatg aatctagacc tgttcttact ggggtgcata
     9841 tagagttatc taataataag ttaatttgtg ctgcaactga ctcacataga ctagcgatac
     9901 gtgaaacatt gctttcctca gatgtaaaag caaattgtat tgtaccaagc gcaaccatta
     9961 gtgaacttct aaaattaatg aacagcaatt cagaattcgt atatatctat ctttcagaga
    10021 gtcatattat atttacgctc ggaacaacta cattatattc aagactaatc gaaggtaaat
    10081 atcctaatat ttctaatctt attccgaacg actttaaaac gattattaac atagatagaa
    10141 aaaagatatt acaaggtgta gaccgatcaa gtttattagc aagtgaatgg gccaataaca
    10201 atgttaactt agaaatcata aacgaatcta caattaaaat ttcttcaaa
//
