LOCUS       NC_022873              10330 bp    DNA     circular CON 15-AUG-2024
DEFINITION  Bacillus thuringiensis YBT-1518, complete sequence.
ACCESSION   NC_022873
VERSION     NC_022873.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis YBT-1518
  ORGANISM  Bacillus thuringiensis YBT-1518
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 6002284)
  AUTHORS   Wang,P., Zhang,C., Guo,M., Guo,S., Zhu,Y., Zheng,J., Zhu,L.,
            Ruan,L., Peng,D. and Sun,M.
  TITLE     Complete genome sequence of Bacillus thuringiensis YBT-1518, a
            typical strain with high toxicity to nematode
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6002284)
  AUTHORS   Wang,P., Zhang,C., Guo,M., Guo,S., Zhu,Y., Zheng,J., Zhu,L.,
            Ruan,L., Peng,D. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2013) State Key Laboratory of Agricultural
            Microbiology, College of Life Science and Technology, Huazhong
            Agricultural University, 1 Shizishan Road, Wuhan, Hubei 430070, P.
            R. China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 1.04
            Reference-guided Assembly         :: NC_005957.1
            Genome Coverage                   :: 190X
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000497525.1-RS_2024_08_15
            Annotation Date                   :: 08/15/2024 02:37:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,968
            CDSs (total)                      :: 6,823
            Genes (coding)                    :: 6,362
            CDSs (with protein)               :: 6,362
            Genes (RNA)                       :: 145
            rRNAs                             :: 15, 15, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 15, 15 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 461
            CDSs (without protein)            :: 461
            Pseudo Genes (ambiguous residues) :: 0 of 461
            Pseudo Genes (frameshifted)       :: 191 of 461
            Pseudo Genes (incomplete)         :: 266 of 461
            Pseudo Genes (internal stop)      :: 128 of 461
            Pseudo Genes (multiple problems)  :: 100 of 461
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:11:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2857844
            Orig. end                         :: 2868174
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP005935.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10330
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10330
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10330
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5330](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5330
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    286..300
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.35"
                     /tool="antismash"
     gene            376..1824
                     /locus_tag="YBT1518_RS14365"
                     /old_locus_tag="YBT1518_14390"
     CDS             376..1824
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-469). E-value:
                     1.7e-29. Score: 95.0. Matches aSDomain:
                     nrpspksdomains_YBT1518_RS14365_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="YBT1518_RS14365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14390"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_023522050.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.8e-27, bitscore:
                     94.9, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYLKKMIMKGHLLGDQKIPSTRELSEL
                     LSVSRNTVLAAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTALADE
                     LDLMKHGVRWEKGMIVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYRPL
                     MNYLLHYMEMKGVDISNKDILITNGFTEGLDIVLSSLSKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMHEDGIDTNEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYNVPIIEDGFNEELRYSGSHLAPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQY
                     IPFKRMTGDGGLHLFIELEEHMNARTLLQKCYEKGVTFSPGDVFYPDGEGANTFRLGFS
                     RLKEEEIVRGIKIIGDTLKNENWS"
     aSDomain        811..1782
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_YBT1518_RS14365_Aminotran_1_2.1"
                     /evalue="1.70E-29"
                     /label="YBT1518_RS14365_Aminotran_1_2.1"
                     /locus_tag="YBT1518_RS14365"
                     /protein_end="469"
                     /protein_start="145"
                     /score="95.0"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYRPLMNYLLHYMEMKGVDISNKDILITNGFT
                     EGLDIVLSSLSKKSGRVICENPTHHAALKLFRLHGLEVHGIDMHEDGIDTNEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYNVPIIEDGFNEELRYSGSHLAPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQYIPFKRMTGDGGLHLFIELEEHMNARTL
                     LQKCYEKGVTFSPGDVFYPDGEGANTFRLGFSRLKEEEIVRGI"
     gene            1829..2743
                     /locus_tag="YBT1518_RS14370"
                     /old_locus_tag="YBT1518_14395"
     CDS             1829..2743
                     /EC_number="6.3.2.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="YBT1518_RS14370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14395"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_003270605.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 9.5e-22,
                     bitscore: 76.0, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 1.4e-13, bitscore:
                     49.7, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 1.4e-08, bitscore: 33.0,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 5e-53, bitscore:
                     178.1, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 1.4e-05, bitscore:
                     23.2, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRY
                     EGVETPDWIELTKMEDLNLDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSILDGKQLPIVSIRHAAEFFDYNAKYDDTSTVEEVIELPA
                     EINERVNKASLACYKALKCSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAG
                     ISYSKLLDMIIETSLKVRNEEGF"
     misc_feature    complement(2766..2778)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     regulatory      2817..2922
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            3050..3739
                     /gene="tenA"
                     /locus_tag="YBT1518_RS14375"
                     /old_locus_tag="YBT1518_14400"
     CDS             3050..3739
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="YBT1518_RS14375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14400"
                     /product="thiaminase II"
                     /protein_id="WP_023522051.1"
                     /transl_table=11
                     /translation="MTFSQSLRKEVDSIWEASFNHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITKEEKDNFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGEHLKECQPEEPIYNEWISAY
                     GSDWFRTLVEEQITRLDTIAEKVTEADRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDK
                     DVKDVIG"
     misc_feature    3901..3914
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.67"
                     /tool="antismash"
     gene            4189..4452
                     /locus_tag="YBT1518_RS14380"
                     /old_locus_tag="YBT1518_14410"
     CDS             4189..4452
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101034.1"
                     /locus_tag="YBT1518_RS14380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14410"
                     /product="DUF3937 domain-containing protein"
                     /protein_id="WP_002082716.1"
                     /transl_table=11
                     /translation="MNFERCYMFTNKKLIRFGLSLFVFLGIINFTISYFQTYLETAADI
                     KWVIPEIWKTILLDVPQGILVLLGAIALYDFTKEASQKDASI"
     gene            5001..5330
                     /locus_tag="YBT1518_RS14385"
                     /old_locus_tag="YBT1518_14415"
     CDS             5001..5330
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071363.1"
                     /locus_tag="YBT1518_RS14385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14415"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001071355.1"
                     /sec_met_domain="TIGR03601 (E-value: 6.5e-39, bitscore:
                     130.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQALSLNDYQSGNIVYWDQQNQYPYYYIEDAARRCGGC
                     GRCGGCGGGRCGGFRCGGFRCIGCFGCFGCGGCGGCGGCGGCSNCFDGFSGTTDTTGTI
                     ITYEY"
     gene            <5473..5604
                     /locus_tag="YBT1518_RS37975"
                     /pseudo=""
     CDS             <5473..5604
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016077325.1"
                     /locus_tag="YBT1518_RS37975"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="site-specific integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MYLPTKNIAIINGYIQSFK"
     misc_feature    complement(5764..5782)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.87"
                     /tool="antismash"
     gene            5814..6299
                     /locus_tag="YBT1518_RS14390"
                     /old_locus_tag="YBT1518_14420"
     CDS             5814..6299
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124614.1"
                     /locus_tag="YBT1518_RS14390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14420"
                     /product="hypothetical protein"
                     /protein_id="WP_003270601.1"
                     /transl_table=11
                     /translation="MNMIELKSIIHSYKLKRKIAKDLYGKRDELTLLLNEFNKMKCTVT
                     SEKKKNNILSRLQLIYQNMKLDKQYPLSVAFDSKLLERLEKEPLHTIEDGVTCLHLMLD
                     ENYEKIKQYGSSTSRSFVPLSQSSICLADCICLTGVVVGLLGAISFGGLLLSICSIT"
     misc_feature    6606..6619
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.3"
                     /tool="antismash"
     gene            6611..8998
                     /locus_tag="YBT1518_RS14395"
                     /old_locus_tag="YBT1518_14425"
     CDS             6611..8998
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736192.1"
                     /locus_tag="YBT1518_RS14395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14425"
                     /product="RNaseH domain-containing protein"
                     /protein_id="WP_023522053.1"
                     /transl_table=11
                     /translation="MKKLRLLTFENIVEPLLNEKVSFIYFPIEWLDIVEIHYKTFLLTS
                     KLKRLNERLYDMFSDILFIQHNPYVLNENTPWIVSKEPIRQEQLDYIFQSWYEIIHDWK
                     PNRLVEPPKYEWQSDLISNLPVLHDNETYSKWVPALISHIFCERPIYLENTNEEEIYFS
                     PLRSQNICEAMSGPIKDEKTQDFFSYVYRFECITRGGENAPLLNISIGIRRFYQEYNHP
                     DIPLLLRRKRGMILISTSEFASNNKKIRFVKLKIQQATTGMKWIKIFRNLKDDFQIGGE
                     VDLDHILQYPKDYIVGENKRVLFPYNERIYKVQGTKIEPGITVKEREYLFKEFQRKFSY
                     FTILPECKKVATNNKNELFPLFAPKGLETLILEVWSENIALEIEQALLDSEIVLTKVGE
                     NSYVLNTDFPVLLKIVRYNIEKVLQNPYKMQYCQKYKTNLVDDVKKAVYATAGKRSDAT
                     LALIAMKNCDGREKIDPKQIIREGFARTNRISTFINLFIGQNVSRKTIVNSIFSLLEQK
                     GFLRRSWNKINLPCTYVSLSIERISKFDFLPIFSKIKGKEILYKLYGSTEWQTIDYVLL
                     NINKHNAFLPQPSKRNDMGALFKQYVFETLMKIVQYAKEQNEQVYFIVDANLRKYWIKE
                     LQNKEINIDILPDIVPEVLKVPNLNIIRINTGFDVPSYGVIESDDALDSIGLYADQKGM
                     YYSTGEYSLNCSGIFQRYILEILPLGVKPVERDYIAKMIHYMCCNSSMLSKKNVHMTYS
                     VHMEKVMKTYITDIDAREFKEFDDELDVDVVKVEKKDSITLL"
     gene            9205..9969
                     /locus_tag="YBT1518_RS14400"
                     /old_locus_tag="YBT1518_14430"
     CDS             9205..9969
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017560251.1"
                     /locus_tag="YBT1518_RS14400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_14430"
                     /product="DUF3959 family protein"
                     /protein_id="WP_023522054.1"
                     /transl_table=11
                     /translation="MKKLDVLLMLLSGLFPIAGLTKQIPLEQSIYIGGLLFFTSFGSYF
                     AKKMYSRICSWIAYAPFITLLLVIWHQDISTSSIIANAKIAACVALIPCLFRFRTYGLT
                     LGLFSLWAALLWDIKEVQSLVILERITSLMTSHYVYILLLIGGLVLGGLLAMFIHRKEK
                     DNKENINLLGPKKKRKRLSIKIRLPRLPKLKMKLFKFGGKESKSKMPEKNHEYKYEESV
                     ATYEMTEQIDPYKENAVQGQTRMERRRNRYNA"
ORIGIN
        1 gcacgttaca aaacagcaaa ttcgaaatgc taaggaacaa cataaaatta ttaaattaat
       61 agcatcagca tataaggatg agggtggcaa tgtcaattta aatgttgaac cgtgcgaaat
      121 agaaaaagat catccgttag caaaagtaaa tgggaccgaa aaaggaatta cgttctttac
      181 agatacgatg ggacaagtta ctacaattgg tggggcttct aatccacgag gcgctgcagc
      241 tgcagcttta aaagatgtaa ttaacttata tcgaaaagat ttgtaagacc gtaccgccct
      301 ttataaaaaa tacatatgtc gtggaccttt tgagaatgaa ataaatatgc aaaaatgatt
      361 gcagggggta aggaattgtt taaggatttt aaagttgtta aagatcgtcc cgtttatatt
      421 caattgaaag attatttgaa aaagatgatt atgaaagggc atttgctcgg ggatcaaaaa
      481 atcccatcaa caagggaact aagtgaatta ctaagtgtga gtagaaacac tgtactcgct
      541 gcttatgcag atttagagca agaaggactc atttatgcag ttaaaggaaa agggaacttt
      601 gttgcgaagg tggatatatc taacacttcg tctgttgaaa tagattggaa aaataaactt
      661 aatacagtga ccgcattagc ggacgaatta gatttaatga aacatggtgt tcgttgggaa
      721 aagggaatga ttgtttttaa tagtatagcc ccggatgaga agctatttga tgttgaaaat
      781 ttcaaaagag cttttcttac tcgtatgtcc attgaagggg acatcgtatt gaactacgga
      841 tatgcaaagg gatatagacc gttaatgaat tatttacttc attacatgga aatgaaaggt
      901 gtagacattt cgaataaaga tattctaatt acaaatggat ttacagaagg attggatatt
      961 gtactctctt ctttatcaaa aaaatccggt cgtgtcattt gtgagaatcc aactcaccat
     1021 gctgcactta agcttttccg tttacatgga cttgaagttc atgggattga tatgcatgag
     1081 gatggtattg atacgaatga agttgaaaaa agtttgcgtg aaaaagattt tgattttgct
     1141 tatttaattc cttcttatca taatccaacc ggtattgtta cgagttcaga gaaaagaacg
     1201 gaattgatga ggttattctc aaaatacaac gttcctatta tagaggacgg atttaatgaa
     1261 gaattacgtt attcaggttc acatttagct ccattattaa cttttgctgg tgctggcaat
     1321 aatgtgattt atattagtag cttttcaaag gtacttttcc ctggtttacg tgtagggtgg
     1381 attattgcag ataaagagct gattcattat ttagaaagcg taaaaagagc aagaacgatt
     1441 cacacatcta cgctagatca agctgttctg tttcaatatt tacatgaggg atattttgaa
     1501 aaatatttaa aaaaggcaag atctgtttat aagaaaaaat atgagttagc tgtcggggca
     1561 tgtaaccagt acattccttt taaaagaatg actggagatg gtggactgca tttatttata
     1621 gagctagaag agcatatgaa cgcccgcaca cttttacaaa agtgttatga gaaaggcgtt
     1681 acgttttcac ctggagatgt tttttaccct gatggagaag gagcgaacac tttccgatta
     1741 gggttttcgc gcttgaaaga agaagaaata gttcggggaa ttaaaataat tggtgataca
     1801 ttaaaaaatg aaaattggag ttgagaaaat gaaaatcggt gttattatgg gcggggtatc
     1861 gtctgaaaaa caagtttcaa ttatgaccgg gaatgaaatg attgctcatt tagataaaaa
     1921 taagtatgaa atagtcccaa ttacattaaa tgaaaaaatg gatttaattg aaaaagcgaa
     1981 agacattgac tttgcattgc tcgcattaca cggaaaatac ggagaagatg ggacagttca
     2041 aggaacactg gagagtttag ggattcctta tagcggaagt aatatgttat ctagtagtat
     2101 atgtatggat aaaaatattt cgaaaaagat tttacgttat gaaggtgtag aaacaccaga
     2161 ttggattgaa cttacaaaaa tggaggatct aaatcttgat gagttagata agttaggatt
     2221 tccattagtg gtaaaaccaa actctggcgg atcgagtgtc ggggtaaaaa tcgtttacaa
     2281 taaaaatgaa ttgatctcaa tgctagaaac agtattcgaa tgggattctg aagtagtaat
     2341 tgagaagtat ataaaaggtg atgaaattac atgttctatt ctggatggaa aacagttacc
     2401 tatagtttca attcgacatg cagccgagtt ctttgattac aatgcaaaat atgatgatac
     2461 tagtacagtt gaggaagtta tcgaacttcc agcagaaatt aatgaacgtg taaataaagc
     2521 ttcactggct tgttataaag cattaaaatg tagtgtttat gcaagagttg atatgatggt
     2581 gaaggacgga attccatatg taatggaaat taatacatta ccagggatga cacaatcaag
     2641 cctattgccc aaaagtgcag aagcagcggg aattagttat agcaaactat tagatatgat
     2701 tattgaaaca tcattaaaag ttagaaatga agaagggttt taaggagatt atataactaa
     2761 ttcttttgac agtttcacga agtatgctat gatgaataca ttataaaaac aaaatattta
     2821 tccactaggg gggcctttta taggctgaga tcaaatgtga ttttgagact cttagtacct
     2881 gatctggtta atgccagcgt agggaagtgg gaaagacgtt gctatttaat gagtaaataa
     2941 atactgtcaa tttcactttc tttacgcctg taaagaaagt ttttttattt tacagcttta
     3001 ataattgtgg ataaatactt atattttata cattattgga gggatttaaa tgactttttc
     3061 acaatcatta cgtaaagaag tagattcaat ttgggaagcg agttttaacc atccttttgt
     3121 aaaaaaactt ggtgaaggaa cattagattt agctagtttt cgctattatg tgcttcaaga
     3181 ttcatattat ttgagtcatt ttgctagagt acaaacttta ggagctgcaa aagctcttga
     3241 attagagacg acagctcgta tggcacatca tgctcaaaat acatatgagg ccgagttatc
     3301 tttacatgag aattttgcta aaaagctagg gataacaaaa gaagaaaaag ataattttat
     3361 tcctgcgcca actgcatatg cgtatacttc acatatgtat cgtgctgcgt atgaagggca
     3421 tttaggagat atcattgcag caattttacc ttgctattgg ttgtattatg aaattggcga
     3481 acacttaaaa gagtgccagc cggaagagcc aatttataac gaatggattt ctgcttatgg
     3541 atctgattgg ttccgtacgc tagttgaaga acaaattaca cgtttagata ctatcgctga
     3601 aaaagtaaca gaggcagacc gcaaacgtat gaaacagcat tttattatta gcagtcaata
     3661 tgaatattca ttttgggaaa tggcttatac attagaaaag tggccagtgg ataaagacgt
     3721 aaaagacgta attggataag gaatagaagt aatgttatag ataggtatgg ataatatcta
     3781 tctatatatt tttttgtcaa atttgtacaa agttgatagt actgcagtta tagcgaaatg
     3841 acatatcgat aagttagttt agttgtctac gcattaaatg gataaactac tgtcacattt
     3901 attttacaat aaaacggata tgttactaag ctaacttcag atacacaacg taatgagatg
     3961 aaattttacc ttgaacgtaa agcttggagg tatgaagtta gataagaaaa gaaacccgca
     4021 ctgttagctg aacggagttg ttttttcttt gcaggaattt ctaaattacc atgaagtgct
     4081 gttaacacaa tatgcatatt tgcatattgt gttttttttg agttcttttt gtgttaaaat
     4141 caaatatagg gatatggaaa tataaagaag taaaagcagg aatatccaat gaattttgag
     4201 aggtgttata tgtttacaaa taagaaatta attcgatttg gtttatcgtt gtttgtattt
     4261 ttagggataa ttaattttac aatcagctat ttccaaacat atcttgaaac agcagcagat
     4321 attaaatggg taattccgga aatttggaaa actattttac tagatgttcc tcaaggtata
     4381 cttgttcttt taggcgcaat tgctttatat gatttcacaa aagaggcatc acaaaaagac
     4441 gcatcaatct aagtgtgtct ttttctatta ggaatttcat agagagtttt gctggagaaa
     4501 tagaatgaaa agccctaata agggcttttt tctttgtagg aatttcttat ccgtcatata
     4561 aaccggtcac taggagatgt tatagcgaaa cgcatttaca ttacagaaaa atctaagcgg
     4621 cagacgcaaa tatataggat atggcaacat aattattgag aaattaattg aatttagtag
     4681 gtatgaagat atgacgctag aattcatatt ttttaatttt tatttatccc gcattaacgg
     4741 gctgttcgta aaagcctgat tggtgagggc taataatcag cgggggaaga actccccccc
     4801 actgattaaa gtttcacttt ataaaaatga gcccatagca aaaataggta tatgaacaag
     4861 gtgcatcatt tagtgataag tcatactttg ttggtgtacg tattgaactt agtttatagc
     4921 gctattttta cttagatgta acgaacagaa atatagggaa gcatcaatga ggttgtattt
     4981 tataaacgga ggttatgaaa atgaatcagt ttcaacaaga actacaagcg ttaagtctta
     5041 atgattacca atctggaaat attgtctatt gggaccagca aaatcaatat ccatattact
     5101 atattgaaga tgctgctcgt cgctgtggcg gttgtggtcg ttgtggtggt tgtggtggag
     5161 gtcgttgtgg cggatttcgt tgtggtggat tccgttgtat tggttgcttc ggttgttttg
     5221 gttgcggagg ttgtggcggc tgtggtggct gtggtggttg ctctaactgt tttgatggtt
     5281 ttagtggtac tactgatact actggtacta ttataacgta tgaatattga ttgcatttat
     5341 cctgctattt gccgggcagt aagaccctaa aaaaattcag cgagagcaaa gtggcggact
     5401 acccataaaa gcccgattgg tgaaggctaa ttaaagcttc actttatcaa ttaaaaaaca
     5461 ttagttttat atatgtactt gccgacaaag aatatagcca ttattaatgg atatattcaa
     5521 tcattcaagt gaaagagtaa caataagata tatagatgta atcaagaggt gatggataaa
     5581 gcaatgacta gatttaaaat ctaatcattg ctcttttctt atggttttat catcaaatat
     5641 ctgtctagtt tagggattga tttcttctag ctaatgaatt tattacaact ttgtaaggtt
     5701 aatgtaatgt taatcgatag taaatgaaga tgcttgagtg taatttaaat aagattaaaa
     5761 agtactgttt atctataaaa taatcgggta aataatatgt aattaggaag tgtatgaata
     5821 tgattgaact aaaatctatt atccattcgt ataaactaaa gagaaaaata gctaaagatt
     5881 tatacggaaa aagagatgaa ttgacactgt tattaaatga atttaataaa atgaaatgta
     5941 cagtaacatc tgaaaagaag aaaaacaata tattatctcg tttgcaacta atttatcaaa
     6001 atatgaaatt agataagcag tatcctctat cagttgcttt tgatagtaaa ttattggagc
     6061 gattagaaaa agaacctcta catactattg aggatggtgt aacatgttta catctaatgt
     6121 tagatgagaa ttatgagaaa ataaaacaat atggttcgag tacaagtagg tcatttgttc
     6181 cattatcgca gtcttctatt tgtcttgctg attgtatttg tttaacagga gttgtagtag
     6241 gtttactagg agcaatttca tttgggggat tactgttatc tatatgttca attacataaa
     6301 gattgatatt tgtaaaaagg gcaccgaatt aaggtgttct tttttggttt ttttgtaaag
     6361 ttatcgtaaa tgataagtac aatgcattag atgtaatgta taattcgaat gtattgaaat
     6421 acaaatataa tagaggtgtt atacatgata attactttga atttttaaaa gagtcggttt
     6481 gataataatt tatgtaaata aacagttttt acgtttcttt taaatacttg gtttaaatgg
     6541 attagattaa gtaactattg ttctagcttt ttgataatca agacaactga agaaaggaag
     6601 gaatcatttt atgaaaaaat taaggctgct aacatttgaa aatatagtag aacccctttt
     6661 aaatgaaaag gtatcattta tatactttcc tattgaatgg ctggacatcg tagagataca
     6721 ttataagaca tttttattaa cgagtaagtt gaaacgtttg aatgaaagat tgtatgatat
     6781 gttttcggat atattgttta ttcagcataa cccgtacgta ttaaatgaaa atacaccatg
     6841 gattgtatcg aaagaaccta ttagacaaga gcagctcgat tatatttttc aaagttggta
     6901 tgagattatt catgattgga aaccaaatag attagtagaa ccaccaaaat atgaatggca
     6961 atccgatttg atttctaatt tgccagtact acatgataat gaaacgtatt ctaagtgggt
     7021 gcccgcttta atctcacata ttttttgtga gcgtcctata tatttagaaa atacaaatga
     7081 agaagaaatc tatttttccc ctcttagatc acaaaatatt tgtgaggcga tgtctgggcc
     7141 gataaaagat gaaaaaacac aagatttttt ctcctatgta tatcgattcg aatgcataac
     7201 ccgcggtggt gagaacgctc cattattaaa tatttcaata ggaattcgga gattttatca
     7261 agaatataat catccagata ttcctttact tttaagacga aaacgaggca tgatattgat
     7321 ttctacctca gagtttgcgt caaataataa aaaaatacgg tttgtaaaac taaaaataca
     7381 acaagctaca accggcatga aatggattaa gatatttaga aatttaaaag atgattttca
     7441 aataggggga gaggtggatt tagaccatat tctccaatat ccgaaagatt atattgtagg
     7501 agaaaataaa agggtgttat ttccatacaa tgagagaatt tataaagttc aaggtactaa
     7561 aatagaaccg ggtataacag taaaggaaag agagtattta tttaaagaat ttcagcgtaa
     7621 attctcttat ttcacaatac ttccagaatg taaaaaggta gctacaaata acaaaaatga
     7681 attatttcct ttatttgcac caaaaggatt agagacatta atattagaag tttggtctga
     7741 aaatatagcg ctggaaatag aacaagcatt acttgatagc gaaattgttt tgactaaagt
     7801 tggtgaaaat tcttatgtat tgaatacaga ttttccagtg ttattaaaga ttgtgagata
     7861 caatattgaa aaggtgttac aaaatccata taaaatgcaa tattgccaaa aatataaaac
     7921 aaatcttgta gatgatgtta agaaagctgt ctatgctacc gcgggtaagc gcagtgatgc
     7981 gacattagca ttaatcgcga tgaagaattg tgatggacgc gaaaaaattg atccaaaaca
     8041 aatcattaga gagggatttg cacgaacaaa ccgtatttca acatttatta atttatttat
     8101 aggacaaaat gtatctcgta aaacaattgt aaatagtatt tttagtctat tagaacaaaa
     8161 aggatttttg aggcgtagtt ggaataagat aaatttacct tgtacatatg ttagtttatc
     8221 aattgaacga atatcgaaat tcgatttctt acctattttt tcaaaaataa aaggaaaaga
     8281 aattttatat aaattatatg ggagtacaga gtggcaaacg attgattacg tattattaaa
     8341 tataaataaa cataatgcat ttttaccaca accatcaaag agaaatgata tgggggctct
     8401 ttttaaacaa tatgttttcg aaacattaat gaaaattgta caatatgcaa aagaacaaaa
     8461 tgaacaagtt tacttcattg tagatgcaaa tttgagaaaa tattggataa aagagttaca
     8521 aaataaagaa attaatatag atattttacc tgatattgtt ccagaggtgt taaaggttcc
     8581 aaatttaaat attattagaa taaatacagg ttttgatgta ccaagttacg gtgtaataga
     8641 atctgatgat gctctagata gcattggttt atatgcagat caaaaaggga tgtattatag
     8701 cactggtgaa tattcactta attgtagtgg gatttttcag cgatatatac ttgaaatttt
     8761 accattgggt gtaaaacctg tagaaaggga ttatatcgct aaaatgatac attacatgtg
     8821 ctgtaattct agtatgcttt cgaaaaagaa tgtacacatg acatattccg tacatatgga
     8881 aaaagtaatg aagacttata ttactgacat agatgcaaga gagtttaaag aatttgatga
     8941 tgaattggat gtagatgtag taaaggtaga aaagaaagat tcaattactc tactttaaat
     9001 actagtaaac aaatcctgtt cgttatatcg atcaggattt gtcatttttt tatgtaaata
     9061 aggatatcct ttgtcaagta tcttttcgtt tacatattta ctttatgtgc agacaggatt
     9121 aatatgtttt ttagaaatac aaaaactata tcatatggaa aaagattttt attaattgaa
     9181 aaaatttgaa aggaatgggg aattgtgaag aaattagatg tgctcttaat gctattgagc
     9241 ggcctctttc caattgcagg acttacaaag caaatccctc tagaacaatc tatatatatt
     9301 ggagggcttt tattttttac tagtttcggt agttattttg ctaaaaagat gtattcacgt
     9361 atatgcagct ggatagcgta tgcgccattt ataacattac tgttagttat ttggcaccag
     9421 gatatttcaa caagttcaat catagcaaat gcgaaaattg ctgcatgtgt cgcgttaatt
     9481 ccatgtttat tccgttttcg tacatatgga cttactttgg gcttattctc attatgggcc
     9541 gctttactat gggatataaa agaagtacag tcgttagtca tacttgaacg cataacgagt
     9601 ttaatgacaa gccattatgt atatatatta ctattaattg gaggacttgt attaggagga
     9661 ttacttgcga tgttcattca ccgcaaagag aaagataata aagaaaatat aaatctactt
     9721 ggaccaaaaa agaaacgcaa aagattatca attaagattc gacttccaag attgccgaaa
     9781 ttaaagatga aactatttaa gtttggtggg aaagaatcta aaagtaaaat gccagaaaag
     9841 aaccatgagt ataagtacga agaatcagtt gcaacatatg aaatgacaga gcaaatagat
     9901 ccatacaaag aaaatgcagt tcaaggtcaa acaaggatgg aacgccgaag aaataggtat
     9961 aatgcataat gaagtttaat aatcccgtaa tgacttatct taatattgat aaatcattac
    10021 gggatttttt atgtaattaa atgaaggttg ctaatgtggg gggaacagca tattttatag
    10081 gtaggaacca aaatgaaata aggggaggat aaaatgctta tcattacata tatatctgct
    10141 tgtatattat tcttagttag ctttttacat gtatattggg ctttcggagg taattggggg
    10201 acaaatagtg ttattccaac taaatcaggg gaaaaggcgt ttacacctcg cgccggaatg
    10261 acattgttca ttgcattatt attaagtatg gcagcaataa ttttgttgca acaagcaaaa
    10321 attgttcatt
//
