LOCUS       NC_022873              12244 bp    DNA     circular CON 15-AUG-2024
DEFINITION  Bacillus thuringiensis YBT-1518, complete sequence.
ACCESSION   NC_022873
VERSION     NC_022873.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis YBT-1518
  ORGANISM  Bacillus thuringiensis YBT-1518
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 6002284)
  AUTHORS   Wang,P., Zhang,C., Guo,M., Guo,S., Zhu,Y., Zheng,J., Zhu,L.,
            Ruan,L., Peng,D. and Sun,M.
  TITLE     Complete genome sequence of Bacillus thuringiensis YBT-1518, a
            typical strain with high toxicity to nematode
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6002284)
  AUTHORS   Wang,P., Zhang,C., Guo,M., Guo,S., Zhu,Y., Zheng,J., Zhu,L.,
            Ruan,L., Peng,D. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2013) State Key Laboratory of Agricultural
            Microbiology, College of Life Science and Technology, Huazhong
            Agricultural University, 1 Shizishan Road, Wuhan, Hubei 430070, P.
            R. China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 1.04
            Reference-guided Assembly         :: NC_005957.1
            Genome Coverage                   :: 190X
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000497525.1-RS_2024_08_15
            Annotation Date                   :: 08/15/2024 02:37:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,968
            CDSs (total)                      :: 6,823
            Genes (coding)                    :: 6,362
            CDSs (with protein)               :: 6,362
            Genes (RNA)                       :: 145
            rRNAs                             :: 15, 15, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 15, 15, 15 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 461
            CDSs (without protein)            :: 461
            Pseudo Genes (ambiguous residues) :: 0 of 461
            Pseudo Genes (frameshifted)       :: 191 of 461
            Pseudo Genes (incomplete)         :: 266 of 461
            Pseudo Genes (internal stop)      :: 128 of 461
            Pseudo Genes (multiple problems)  :: 100 of 461
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:11:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 287471
            Orig. end                         :: 299715
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP005935.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12244
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12244
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12244
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7244](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7244
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(77..91)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 16.66"
                     /tool="antismash"
     misc_feature    complement(742..755)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 22.42"
                     /tool="antismash"
     gene            2018..3286
                     /locus_tag="YBT1518_RS01670"
                     /pseudo=""
     CDS             2018..3286
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243333.1"
                     /locus_tag="YBT1518_RS01670"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="BppU family phage baseplate upper protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRNEEIMIDLADPVFTKTIRSRQNDKNGLRLTVYVREKGEKVDLT
                     GYVVKYEATNHTGVFIRDDAQIVDAKNGVFSYTFTSQAVSTSDDWTAYFVMEKSTERMS
                     TPDIRIILRRDVKEGNIKIENYISEFEIIKKTLDELQKKLNALDIVKKSGDTMTGDLNL
                     HVGLALKRITSKDSTKDLISLAFGSNGSFWIEDRQNSNLNVLHYDNTNKKLNVNTDTNL
                     MKKSNDSFTGLTRFTQNAQLMMAQPDEATGASARGLHYMDKDTDVIVNRGGMGRFKGAK
                     NGEEYLYMGFGATP"
     gene            3347..3577
                     /locus_tag="YBT1518_RS01675"
                     /old_locus_tag="YBT1518_01415"
     CDS             3347..3577
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392440.1"
                     /locus_tag="YBT1518_RS01675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_01415"
                     /product="BhlA/UviB family holin-like peptide"
                     /protein_id="WP_023520881.1"
                     /transl_table=11
                     /translation="MEEQIFNSMIQQGVFAALFVWMLFTTQKKNEQREEQYQKVIEKNQ
                     AVIEEQAKAFGSLSKDVSDIKQKIMGNGDDK"
     gene            3574..4626
                     /locus_tag="YBT1518_RS01680"
                     /old_locus_tag="YBT1518_01420"
     CDS             3574..4626
                     /EC_number="3.5.1.28"
                     /GO_function="GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA]"
                     /GO_process="GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016125209.1"
                     /locus_tag="YBT1518_RS01680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_01420"
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="WP_023520882.1"
                     /transl_table=11
                     /translation="MKKSIKLLASISTAAIIAFTSTGSVFADREMIIPGLPKVEYRNGY
                     GAYEGVVAHSTATSEATAINIRNYEARTWRSAFVHYATDWDETIQIASTKYQAWGAGPA
                     ANKRFVHVELSETSDPIQFKKSYERYVKLLAKILRDRNIDPSIGLWTHKDITYKLGGTD
                     HEDPIDYLRSHGVSESKFRADVLKAYNGNSISVEAKPKQPNEVSDVMNEVGVAYIDGYN
                     VNLRSGPSTTNSVIRKLQKGEAYKVWDKVGNWLNLGGNQWIYNDSSYIRYKEESSSVEG
                     KRVVSKVNDLRFYSKPSWLDRDVVGTVDEGLGFTIEAKVGVDGSSQYKVHNSKGETYYI
                     TTNRIFVYTV"
     gene            5001..7244
                     /locus_tag="YBT1518_RS01685"
                     /old_locus_tag="YBT1518_01425"
     CDS             5001..7244
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258271.1"
                     /locus_tag="YBT1518_RS01685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_01425"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_023520883.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 4e-37, bitscore:
                     125.5, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 2.7e-177, bitscore:
                     589.8, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKYLVKQHDAADCAAACLATVCLYYKKDISITKLRDLLGTDIKG
                     TTLKGLESGAKRLGFDTKAVRVNRETFMSKFSLPAIAHIITKEGLAHFVVVHKITKNSV
                     VFLDPAEGRKKISIIEFFNYFDGILLLLAPNDAFVVERKKKSNTLQTFIRLLMPQKMLF
                     VYSIVASLLSTILGVTSAFFNKILMDEILPYNLKNELTIFGLGFLLVVVFQVALSTIRQ
                     HILLYLSQKIDIQLLLGFFRHTYKLPMKFFSTRKVGDILTRFSDAFTIKNILTSVTLSL
                     CIDLFLAIASAVVLYLMNSTLFGVILLLTSLNAALVFIFKRPYKKINFRKKESDSTLNS
                     HIVETLKGIETIKVHAAEEKTLEKLETEYVKNLRISFKEGVLSNIQNTFSSSISGIGQI
                     LLMCIGASMVMDKDITLGGLMAFVTLSGYFMDPIGRLIHIQLAVQEASVSLARISELYD
                     VETEQLNDNKPKVEEISGDIKLENIKYRYGSRGSALNSVSITIPEGKKVALVGESGSGK
                     TTIAKLLLRYYVPEEGSIRIAGSDIQDLDLYSYRKNISYIPQNIELFSGTIRENITLGI
                     RGGTDEQLRRACNDAGCKEFIEKLPGKYDTYLDEAGGGLSGGEKQKIALARALILNNNF
                     TILDEATSNLDFISEANVYRTLFSKKKHATMLIIAHRLSTIQDCDMIYVLDQGKVVEEG
                     THQSLLENKATYYSLYMSQVSSLPNERDSISQLEYNNKDNTNRRIGDDIVEYS"
     gene            7231..7707
                     /locus_tag="YBT1518_RS01690"
                     /old_locus_tag="YBT1518_01430"
     CDS             7231..7707
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268611.1"
                     /locus_tag="YBT1518_RS01690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_01430"
                     /product="hypothetical protein"
                     /protein_id="WP_023520884.1"
                     /transl_table=11
                     /translation="MSTVEKIDVRGKKTLKLTNALIAKISNASPMEVQKRSHMMQNYIK
                     SHGKSIVGPLVNHSYPEMNGDGQVQIHIELIMQLDSPLHTLDKTYEFSKQLKIENCLFA
                     RFYEKEENIRFAYFKLNVHAFENDMKLNGESYTVYVKQNSDTLMADIFMPLQDE"
     gene            7722..8843
                     /locus_tag="YBT1518_RS01695"
                     /old_locus_tag="YBT1518_01435"
     CDS             7722..8843
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /locus_tag="YBT1518_RS01695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_01435"
                     /product="HlyD family secretion protein"
                     /protein_id="WP_023520885.1"
                     /transl_table=11
                     /translation="MKIYNLNEITDSRILYDKKPPRFMLYIVLIVIVMLIAVITWSMYS
                     TKTHVVKGQGIVTTQNKQFIMSPVSGAVEEIHVEEGKNVKKGDLLLSLKPVQSNLQIEQ
                     LNSQLNHLNERIELLVRAQKNISRGTNDFDKKKSSEIEFYNKLNSLQVKMKEFEVKKVT
                     EDSLKEQNYNEEQIQDYKNKASNKIEQAKYEVILEFTNEKKQLEIEVNKLESQKNALAK
                     SLEEYKIPATEDGKFHLSSKITMGMVLQAGAVIGSMSQRDEDLIIELNLPSAERAKVKI
                     KDDVEMVVAGLNQSEYGIIEGRVISIAEDVTIDEKKESNNIYFKVQIKPKTQFLKNDNN
                     NKVNLTLGMLTEVRIKYGKTTYLKYALEQIGIK"
     gene            9059..9394
                     /locus_tag="YBT1518_RS01700"
                     /old_locus_tag="YBT1518_01440"
     CDS             9059..9394
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067994.1"
                     /locus_tag="YBT1518_RS01700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_01440"
                     /product="hypothetical protein"
                     /protein_id="WP_023520886.1"
                     /transl_table=11
                     /translation="MSNLVLPSNYVEIGNEEMEYIDGGYYISNSSLKGIVFSAVGTGAS
                     VAAIEAGIYAIAAGLASTVPALGWVTGAVLGANAANFAITATRAIASGKGMDIGVGFPT
                     GLTFAVA"
     gene            9485..10348
                     /locus_tag="YBT1518_RS01705"
                     /old_locus_tag="YBT1518_01445"
     CDS             9485..10348
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748173.1"
                     /locus_tag="YBT1518_RS01705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_01445"
                     /product="hypothetical protein"
                     /protein_id="WP_023520887.1"
                     /transl_table=11
                     /translation="MQKKIKFLIMITIIIYINNFVFAYINGYKTLIGVSALWAISPFLL
                     LTIASFILASDYKKDYLIVKKEARISFILKVLSCIVAFYNYKFEIGSLEYIMRFVIIAI
                     LCIINVNLEYKMYRIAKKYIPKLDEEEVKPVSEKEKWNIKNYGRAATLGVGSFILVVTG
                     GMNIVFIAQMSRYYGLICICIFIVFLKMNYDKNMLFYQDKVIGKRIFLKDAFYASLGFG
                     YNCAVAFNFISGNDFIENTALIVGICFLYPTIVTNRKIALRQREVSKVIRDNFEYYYND
                     ENNPYK"
     gene            complement(10807..11145)
                     /locus_tag="YBT1518_RS01710"
                     /old_locus_tag="YBT1518_01450"
     CDS             complement(10807..11145)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119483.1"
                     /locus_tag="YBT1518_RS01710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_01450"
                     /product="hypothetical protein"
                     /protein_id="WP_023520888.1"
                     /transl_table=11
                     /translation="MSYDTIASLQRMQQLEQAQAATGKRLVLKRIYESDKISALIIVLC
                     IIAIPATMCISLLVGLVIYYIREFKRTTYVVKNVATGEKFKVNKQDFRQYKKEVKAKEK
                     EVKKISEL"
     misc_feature    11231..11246
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.45"
                     /tool="antismash"
     misc_feature    complement(11232..11245)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 21.95"
                     /tool="antismash"
     misc_feature    11255..11268
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.69"
                     /tool="antismash"
     gene            11284..11502
                     /locus_tag="YBT1518_RS01715"
                     /old_locus_tag="YBT1518_01455"
     CDS             11284..11502
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016718549.1"
                     /locus_tag="YBT1518_RS01715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_01455"
                     /product="hypothetical protein"
                     /protein_id="WP_023520889.1"
                     /transl_table=11
                     /translation="MNNLLTCSFNQKIPIELIYLNGSGDFSQRTVIVRKICEDRILVYC
                     MEKQQVRTLKLTNILSIDKVRIKYQYA"
     gene            11526..11819
                     /locus_tag="YBT1518_RS01720"
                     /old_locus_tag="YBT1518_01460"
     CDS             11526..11819
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100793.1"
                     /locus_tag="YBT1518_RS01720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="YBT1518_01460"
                     /product="YolD-like family protein"
                     /protein_id="WP_023520890.1"
                     /transl_table=11
                     /translation="MTNGVPKPPKKKSGSKKEPRPYMDEFEQQAAAELIQLAMQDDMGL
                     VFTVYRKYKEPDIIQGKIVKLEQQMGRIVVSDGPNIIYKIQFIDILKIDTPS"
     misc_feature    12027..12043
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: medium, score: 27.47"
                     /tool="antismash"
ORIGIN
        1 gtatgtcagc aggatgccaa tacaagcatc cttttcagtt ttattcagat gtaccaaagg
       61 tagcaagtgc acgtattgtc cgtaaaaatc ctgtggaggc attattagat tctagtcaag
      121 ataattcatt tgttaatcgt tggggtggcg aattaaaacg agataatttt gacgtgaaga
      181 tgctacaaaa tcgtggtatg gatcgcggag tagttattcg tcataagaag gatttgttag
      241 ggtatgaagg taatgtagat tggaaaagtc ccataactag aatcatgcca caaggttttg
      301 atgggttatt tcttcctgaa aaatatgtag atagcccact tataaataag tatcctcatc
      361 ctaaaattaa agtggttgag tttaaacata ttaaagcagc tattggtgaa aatgctgacg
      421 atgaagatgc agttccgtta gaagaagcat ataggttatt acgccaggca gctaaggata
      481 tgtttgctat tcaaaaggtt gatcagccta aagcaaatta taacgttacg tttcaggagt
      541 tatcacaaac ggaagagtat aaggattata agcatttaca aagtgtttat atggcagata
      601 cggttacggt tgagcatcaa gaagatggta ttgatataaa ggcgaaggta attgcttata
      661 aatatgatcc aataaaaaaa gagtatctgg atataaccat tggtaacttc aaagaatcct
      721 ttacggacgt ttccggtaga gttgatctgg tacaagaaga gttatccaat atgccaggct
      781 ctattttgga tgcagcaaaa gcaaatgcta caagccttat taattcagga ttcggaggac
      841 atgtccgcat ttatccagat cgtattttaa ttatggatac gaaagatgaa aagagtgcga
      901 aaaaggtttg gcaatggaac ttgaatggat tagggtattc ttccacaggt gtgaatggac
      961 catatggaac ggccattaca agtgacggaa gaattgttgc tgattttatt actgcaggta
     1021 cgttgaatgg aaatcttgtg caaggtggag aaataacagg tgcaacatta cgaacttcag
     1081 atagcgtcaa ctatgtaaat atttcaaagc aattcatacg cttgtatgag tcatctaaga
     1141 caagggtgtt tgtagggtat tataaaaata gtagaaatga aatacaacct actcttattt
     1201 taggcggaga ttcagattcc acaggggcaa atggtgctat tatggtgtac caattctcag
     1261 atacgagcgt taagtctggt ggaatcggaa tcacaaaagg actcgatggc aatggatact
     1321 tgaatgcagc ttctttatac ttttcgcaaa cagggaatgc aatgctcgat gctgataaaa
     1381 tgattgttct agatgcccaa agtgaaatga gatttaaggt taaagatcag ttccgctttt
     1441 atcgtaatga caattggatt gcgagtatcg gggttgcatc tggaggggat acagatatta
     1501 tgctcccaaa tgcaataata cgaaattcga gttgggacaa tgggtatatc caagtgaaaa
     1561 ccgctcttgg gacatattat caaggagtaa ttgcttcaga cttcaaagtt tcttcaaaag
     1621 aaacctataa aactaatatc cgtcctgttg cattcagcgc acttgaaaag gtaatggaat
     1681 gggaaattaa acagtacaat ttgaaaaccg acattccaaa actgtatgag atgcgtatga
     1741 atcgtaagga aggagagcca acaattacta cagatgcaat tcctacacat tatggtttag
     1801 ttattccaaa agaatcagag gaaaatggtg taggcttata tggaatgctt tcacaattaa
     1861 caagtgcatt tcaggagtat gtagccaaaa cagatgctag aatcgaagaa ttagagccat
     1921 tacaacctaa aggaaatata aaacatagga acagagtaaa acgtcagaga agaccgtcta
     1981 gacatgtaaa aaggagtagc tagaaagagg tgtagtcatg cgaaatgagg aaattatgat
     2041 agatttagca gatcctgtgt ttacaaaaac tattcgttct cggcagaatg acaagaatgg
     2101 attgaggctt acggtgtacg taagagaaaa aggggagaag gtggatttaa caggatatgt
     2161 ggttaaatat gaagcgacaa atcatacggg agtattcatt cgagatgatg ctcaaatagt
     2221 tgatgcaaag aatggtgtgt tttcatatac gtttacatct caagctgttt ctacatcgga
     2281 tgattggaca gcttattttg tgatggaaaa aagtacagaa cgaatgagta caccagacat
     2341 tcgtattata ttaagacgtg atgtgaaaga aggaaatatt aaaatcgaaa attacatttc
     2401 tgagtttgag attattaaga aaacgttaga cgagttgcag aagaaattaa atgctttgga
     2461 tattgttaag aaaagcggag acaccatgac aggtgacctg aatcttcatg taggtttagc
     2521 tttgaagcgt attacgtcta aggatagcac aaaagattta atctcattag cgtttggatc
     2581 aaacggatcg ttttggattg aagaccgtca gaatagcaac ttgaatgttc ttcactacga
     2641 taacactaat aagaaattaa acgtaaatac agataccaat ctcatgaaga agagtaacga
     2701 ttcattcaca gggcttacac gattcaccca gaatgctcag cttatgatgg ctcagcctga
     2761 cgaggctaca ggtgcttctg ctcgtggatt acactacatg gataaagaca cagacgtaat
     2821 cgttaatcgt ggcggaatgg gacgtttcaa aggggctaaa aacggtgaag aatatctgta
     2881 catgggattc ggtgctactc cctgagattc tcaaagtggg ttaatagtgc gacctgatgg
     2941 atcagcaacc ttgaaaggta aaaagatagc aaccgctgaa aatgatacag gatggattaa
     3001 gcttgctaca actggggtag aaaatgttgc taacagagat atgaaataca agagaagtgg
     3061 tgaaaacatt agtgtaattg gttcagttcg aaatcctcaa aatgaggcag tattcactac
     3121 attaccagtt ggatttagac cagtacagca cattgctttt ccagcgctgg catatggata
     3181 tacacccgca gcgtgtgaag tgacaataaa acctgatggg gggattttcg tgaatggtgt
     3241 tccgagcggg agtacggttc atattgcaat gaacttttta atttaaatcg tacaagttaa
     3301 gcgtgcataa gcaggcttat ttattttgct caaggggatg agaacagtgg aggaacaaat
     3361 cttcaattca atgattcaac aaggagtatt cgcagcgtta tttgtgtgga tgctttttac
     3421 tacgcaaaaa aagaatgagc agcgtgaaga acagtatcaa aaagtaatcg aaaaaaacca
     3481 agcagtcatc gaagaacagg caaaagcatt tggttcactt tcaaaggatg tatcagatat
     3541 taaacaaaaa attatgggga atggtgacga caaatgaaaa aatctattaa attattagcc
     3601 tcaatttcta ctgcagctat cattgcattt acttcaacag gtagtgtctt tgctgatcga
     3661 gaaatgatta ttcctgggtt acctaaagtc gaatatcgca atggatatgg agcatatgaa
     3721 ggtgtagtag cacattctac agcaacttct gaagcgactg ctattaatat ccgaaactac
     3781 gaagcaagaa catggcgttc tgcatttgta cattatgcca cggattggga tgaaacaatt
     3841 caaattgctt ctactaagta tcaagcatgg ggagcgggcc cagcggccaa taaacgattt
     3901 gttcatgtag agctctctga aacgagcgac cctattcaat ttaaaaaatc ctatgagcga
     3961 tatgtgaagc tactagctaa gattttgcgt gatagaaaca ttgatccatc cattggcttg
     4021 tggacgcata aagacattac gtataagctt ggcggcacag atcacgaaga tccgattgat
     4081 tatcttcgca gtcatggtgt atcagaatca aagtttcgtg ctgatgtatt aaaagcatat
     4141 aatggaaatt ctatttcagt tgaagcgaaa ccaaagcaac caaatgaagt atctgatgtt
     4201 atgaatgaag tgggagtagc gtatattgat ggatacaatg taaaccttcg ttctggtcca
     4261 tccacaacaa atagtgttat tcgtaaatta caaaaaggtg aagcatataa agtctgggat
     4321 aaagtaggca actggttgaa tcttggagga aatcagtgga tttataatga ttcctcatac
     4381 attcgatata aagaagaatc ttcatctgtg gaaggcaaac gtgttgtttc taaagtgaat
     4441 gacttacgat tttattcaaa accttcctgg ttagatagag acgttgtagg aactgtagat
     4501 gaagggttag gatttacaat tgaggcaaaa gtaggtgtgg atggttcctc gcaatataaa
     4561 gtacataata gtaaagggga aacatactat ataactacaa atagaatttt tgtgtataca
     4621 gtataagaat cgaagtagtg gtttattaaa taagtatatt aaaattctca tttctaatgt
     4681 gacaaattaa ccggaaaagg tgttcttctt tcagaattga aaagaagagc atcttttttt
     4741 attttgagtt ccgtaaaaaa gcaagtctta ttggatcttt aaaatgcaaa taagtatatt
     4801 tcgtaaatat tatacaaaat ccacacaaaa tccacacatt taaatgataa tttaaaaaac
     4861 gttatataaa gtggttaagt tttggggttt acatttcttt ggatggtaac caaagactgt
     4921 ttaacaggag tacgtcatgg agagcatgtt taataaatta actaccagga taaaagtaaa
     4981 agaggtgaaa gagaaaagag atgactaaat atttagtaaa acaacatgat gcagcagatt
     5041 gtgcagccgc ctgtcttgca acagtttgtt tgtattataa aaaagatata tctattacaa
     5101 aattaagaga tttattagga acggatatta aagggactac actaaaggga cttgaatcgg
     5161 gagcgaaaag attaggtttt gatacaaaag cggtgagagt aaatcgtgaa acatttatga
     5221 gtaaattttc tcttccagca attgctcata ttattacgaa agaaggactt gctcattttg
     5281 ttgttgttca taaaattaca aagaacagtg ttgttttttt agatccggca gaaggaagaa
     5341 agaaaatatc aattattgaa ttctttaatt attttgatgg gatcttgtta ttacttgctc
     5401 caaatgatgc atttgtagta gagcgtaaaa agaaaagcaa tactttacaa acttttatac
     5461 ggttgcttat gccacaaaaa atgttatttg tttatagtat tgttgcctcg ctattatcaa
     5521 caatcttagg agtaacatct gcttttttta ataaaatttt aatggatgaa atacttccat
     5581 acaatttaaa aaatgagctt actatttttg gattaggatt tctattggta gttgtttttc
     5641 aagtagcttt atcaacaatt aggcagcata tattattgta cttatcgcaa aaaattgata
     5701 ttcaattatt attaggcttt tttaggcata cgtataagct tcctatgaaa ttcttttcta
     5761 ctcggaaagt cggagatatt ttaacaagat ttagtgatgc atttacgata aaaaatattt
     5821 taacctcagt aaccttgtct ttatgtattg atttattttt agcgattgca tctgcggtag
     5881 tgctgtatct tatgaatagt acactttttg gtgtcattct attattaacc agtttaaatg
     5941 ccgcattagt ttttattttt aaaagacctt ataaaaaaat taattttcga aagaaagaat
     6001 cagattccac cctgaacagt catattgtag aaactttaaa aggaatagaa actattaaag
     6061 tacatgcagc agaagaaaaa acgttagaga aattagagac ggagtacgta aaaaatttaa
     6121 gaattagttt taaagagggt gtattatcaa atattcaaaa cactttctct tccagtattt
     6181 ctggaatagg gcaaatttta cttatgtgta taggtgccag tatggttatg gataaagata
     6241 ttacattagg tggattaatg gcttttgtaa ctttatcggg ctactttatg gatcctattg
     6301 gaagactaat acatattcaa cttgctgttc aagaagcaag tgtgtctctt gcaagaatct
     6361 cagaattgta tgatgtagaa acagagcagc taaatgataa caaaccaaag gtagaggaaa
     6421 tatctggtga tattaaatta gaaaatataa agtatagata cggaagtaga ggttcagctt
     6481 taaatagcgt atccattaca atcccagaag gtaagaaagt ggcgttagta ggagaatcag
     6541 ggagtggtaa aactacaata gctaaattgc tattaaggta ttatgttcct gaagaaggca
     6601 gtattagaat tgctggttca gatattcaag atttggattt gtatagttat agaaaaaata
     6661 tttcatatat tcctcaaaat atagaattgt tttctggaac gattagagaa aatataactt
     6721 taggtataag agggggcact gatgaacagt tacgaagagc ttgtaatgat gctggatgta
     6781 aagagtttat agaaaaatta ccaggtaagt atgatacata tttagatgaa gcgggtggag
     6841 gactttctgg tggtgaaaag caaaaaattg ctttagcgcg tgctctaata ttaaataata
     6901 attttacaat attagatgag gccacaagta atttagactt tattagtgaa gctaatgtat
     6961 atcgtacttt attctctaaa aagaaacacg ctacaatgtt aatcattgca cataggcttt
     7021 caaccattca agattgtgac atgatttatg tgttagatca aggaaaagta gtggaagaag
     7081 gaacccatca atcactactt gagaataaag ctacatatta tagtttgtat atgagtcaag
     7141 tgagtagttt acccaatgaa agagatagta tatcccaatt agagtataac aataaagata
     7201 atactaatag gcgcatagga gatgatattg ttgagtacag ttgagaaaat cgatgtaaga
     7261 ggcaaaaaaa cattaaaact tacgaatgct ttgattgcaa aaattagtaa tgcctcccca
     7321 atggaagttc agaaacggtc acacatgatg caaaattata taaagtcaca tgggaaatcc
     7381 attgtgggtc cattagtgaa tcattcttat ccagagatga atggagatgg acaggtgcaa
     7441 attcatattg aactcattat gcaattagat agcccattgc atactttgga caaaacttat
     7501 gaatttagta aacaattaaa aatagaaaat tgtttgtttg cacgatttta tgaaaaagaa
     7561 gagaacataa gatttgcgta ttttaagcta aatgttcatg catttgaaaa tgatatgaag
     7621 ctaaatggtg agagctacac ggtttatgta aaacaaaata gtgatacatt aatggcagat
     7681 atttttatgc cattacaaga tgaatagggt aggagatcgg gttgaaaata tacaatctaa
     7741 atgagattac cgatagtaga atattatatg ataaaaaacc accgcggttt atgttataca
     7801 ttgttttaat agtaatagta atgttgatag ccgttatcac ttggtcaatg tattcgacta
     7861 agactcatgt tgtaaaaggt caaggcatag ttacaacgca aaataaacaa tttattatgt
     7921 ctcctgtttc aggtgcagtt gaagaaattc atgtagaaga agggaaaaac gttaagaagg
     7981 gggatttact tttatcgtta aaacctgtgc agtcaaattt acaaattgag caattaaata
     8041 gtcagttaaa tcatttaaat gaacgaatag aattgttagt acgagcccag aagaatatat
     8101 cccgaggcac taatgatttt gataagaaaa agagtagtga aattgagttt tataataagt
     8161 taaattcttt acaagtaaaa atgaaagaat ttgaagtcaa aaaggtaacg gaagattccc
     8221 taaaagaaca aaattacaat gaagaacaga ttcaagatta taagaataaa gctagtaata
     8281 agatagaaca agcaaaatat gaagtaattt tagaatttac taatgaaaaa aaacagcttg
     8341 aaatcgaagt aaataagtta gaatctcaaa aaaatgcttt agcaaaaagt cttgaagagt
     8401 ataaaattcc agccactgaa gatggtaaat ttcatttgag cagtaagata actatgggaa
     8461 tggttcttca ggcgggcgct gtaattggaa gtatgtcaca aagagatgag gatttaatta
     8521 tagagttgaa tctaccatca gctgaaagag ctaaggtaaa aattaaagat gatgttgaaa
     8581 tggtagtagc aggtttaaat caaagtgagt atggcattat tgaaggaagg gtaattagta
     8641 ttgcggaaga tgtaacaatt gatgaaaaaa aagaatctaa caatatatat tttaaagttc
     8701 aaatcaaacc taagacacaa tttttaaaga atgataacaa taataaggtg aatttaactt
     8761 taggaatgtt gactgaagta agaattaagt atggaaaaac tacatattta aaatatgcac
     8821 tagaacaaat tggaataaag taagttcttt ttagccgtaa actataactt ttatattttg
     8881 tagatatgga tttgtaggaa gtaattaaac tataactaca ttttaaaagc ataattaagc
     8941 tctctcaatt aataattact cactaagaac attcaggaaa taatatattg aattatttag
     9001 tttttcaagt tgcattataa atgtatttat aaaaaaattg aaagagagtg atatattaat
     9061 gagtaattta gttttacctt ctaactatgt agagattgga aatgaggaga tggaatacat
     9121 agatggtggt tactacatca gcaatagtag tttaaaaggg attgttttct cagccgtagg
     9181 tacaggtgct tcagttgctg cgattgaagc tggcatttat gctattgcag ctggattggc
     9241 ttctacagtt cctgctttag gttgggttac tggagcggta ttaggggcta atgcagcaaa
     9301 ctttgcaata actgctacta gggcgattgc tagcggcaaa ggtatggaca ttggtgtagg
     9361 tttccctaca ggcttaactt ttgcggtagc ttaattatta gataatccat actaatgaag
     9421 taattattag tttgagaggg ttttgttatg tttttaattt gtaggtttaa gatgaggtga
     9481 ttgaatgcag aaaaaaataa aatttttaat aatgattaca attattatat atataaataa
     9541 ttttgttttt gcttatatta atggatataa aactttaatt ggtgttagtg cattatgggc
     9601 tatttcacct ttcctgttgt taacaattgc atcttttata ctagcgagtg attacaaaaa
     9661 agactattta atagtaaaga aagaagcaag aataagtttt attttaaaag ttctttcatg
     9721 tattgttgca ttttacaatt ataaatttga aataggttct ctagaatata ttatgagatt
     9781 tgtaatcata gctattttat gtattataaa tgtaaatctg gaatataaaa tgtatagaat
     9841 agcaaaaaaa tatattccta aattagatga agaggaagtt aagcctgtat ctgagaaaga
     9901 gaaatggaat atcaaaaatt atggaagagc agcgacatta ggggtaggat cgttcatttt
     9961 agtcgtgaca ggtggcatga atattgtatt cattgctcaa atgagtaggt attatgggtt
    10021 aatttgtatt tgtatattca ttgtttttct aaagatgaat tatgataaaa atatgttatt
    10081 ttatcaagat aaggtcatag gaaagcgtat ttttttgaaa gatgcatttt atgcaagttt
    10141 aggatttgga tataattgtg cagtagcttt taattttatc tcggggaatg attttattga
    10201 gaatacggca ttaattgtcg ggatttgctt cttatatcct actattgtaa cgaacagaaa
    10261 gattgcttta agacaaagag aagtgtcgaa agtcattaga gataattttg agtactacta
    10321 taatgacgaa aataatcctt ataaataagg ttaatttgag aacaaatatt taagtaatac
    10381 atttaaaaat ccatctttta ggaagaggtg taaaagtagt ctacagcact tctaaaagat
    10441 ggatttattt ttgttgatat aagataagga cagtaaattc aacctacgtg tatgtgaagt
    10501 gaaaaagaat tgtcttttaa atgaggccat tgaaaaagtc attactttga aaaaacatgc
    10561 attttattgg tatctagaaa tgggaaagag tctgatatct ctatatagta agtggactct
    10621 ttcagtgacc acgttttaaa tagacgtttt tttatttcta cgtaattaaa atttacatag
    10681 aaataaatgc ttttaatata ggattgatgg ttttattcaa caaacgaaat aagttaaata
    10741 ttgaacgaat aactgttata aatttagagt tcgtcttcat ttaatatatc tttcctttac
    10801 ctccaattat agttcggata tttttttaac ttctttttcc tttgctttaa cttctttttt
    10861 gtattgtctg aaatcttgct tattaacttt gaatttctct ccagtagcaa cattttttac
    10921 tacatatgtt gtacgcttga attctctaat gtaatagatg actaatccaa ctaaaaggga
    10981 tatacacatt gttgctggga tagcgataat acaaagaacg ataatgagag ctgaaatttt
    11041 atctgattca taaattcttt tcagaactaa acgttttcca gttgcagctt gtgcttgctc
    11101 taattgttgc atacgttgta gtgatgcaat cgtgtcataa ctcatgaaaa tcccctcctt
    11161 taaatcatac ctaaatcata ccaatttcat gtaatacctg taaatgtgcg tttctaattt
    11221 attgacggat tagaacgaat gttctataat tatgcaaacg aacgttcttg cggaggtcat
    11281 catatgaata atttgcttac atgctctttt aatcaaaaaa taccaattga actaatatat
    11341 ttaaacggtt caggagattt ctctcaaaga actgttattg ttagaaaaat atgtgaggat
    11401 cgtatattgg tgtattgcat ggaaaaacaa caagttagaa cgttgaaatt aactaatata
    11461 ctgtctattg ataaagtaag aataaaatat caatacgctt aaaatgctca atgaggtgat
    11521 atgatatgac aaacggagta ccaaaaccac caaagaagaa aagtggttca aaaaaggaac
    11581 ctagacccta catggatgaa tttgaacaac aagcagctgc tgaattaatt caattagcaa
    11641 tgcaagatga tatgggatta gtgtttacag tgtatcgaaa atataaagaa ccagatatca
    11701 ttcaagggaa aatcgttaag cttgaacaac aaatgggtag aattgtagtg agtgatggcc
    11761 caaatataat atataaaatt caattcatag atattttaaa aatcgacacg cctagttaat
    11821 ttagctaggt gttttttata tggccataaa ttctttattg aagtagaact tgtccattaa
    11881 tttatttaca atcccgttga aataagcgca tttacccttg ttcatttttg tgcacgcttt
    11941 aattttcata ataaactctt taatagcttt taacccaata gtaagctcct ggtcctgatc
    12001 aaatgcttta tcacctgtag agaagtttgt aactttatta cactgcctta cgaccttcca
    12061 cagttcttga attgtcttag attcactata aaaagaacta accaaagaaa caaaacgttc
    12121 tggtacccag tgagcaacaa aatcagcttg ttcaatattt tcttctggag tattgctatt
    12181 ctcattacta ttacgtttgt ttatatcttt tatattttgt tttaaggaaa caggttttgt
    12241 ttta
//
