LOCUS       NZ_CM002285            10405 bp    DNA     linear   CON 29-APR-2024
DEFINITION  Streptomyces roseochromogenus subsp. oscitans DS 12.976 chromosome,
            whole genome shotgun sequence.
ACCESSION   NZ_CM002285
VERSION     NZ_CM002285.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseochromogenus subsp. oscitans DS 12.976
  ORGANISM  Streptomyces roseochromogenus subsp. oscitans DS 12.976
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9663240)
  AUTHORS   Ruckert,C., Kalinowski,J., Heide,L. and Apel,A.K.
  TITLE     Draft Genome Sequence of Streptomyces roseochromogenes subsp.
            oscitans DS 12.976, Producer of the Aminocoumarin Antibiotic
            Clorobiocin
  JOURNAL   Genome Announc 2 (1), e01147-13 (2014)
   PUBMED   24407645
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663240)
  AUTHORS   Ruckert,C., Szczepanowski,R., Heide,L., Kalinowski,J. and Apel,A.K.
  TITLE     Draft genome sequence of Streptomyces roseochromogenes subsp.
            oscitans DS 12.976
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 9663240)
  AUTHORS   Ruckert,C., Szczepanowski,R., Heide,L., Kalinowski,J. and Apel,A.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2013) CeBiTec, Bielefeld University,
            Universitaetsstr. 27, Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.5.3
            Assembly Name                     :: S.roseochromogenes_v1
            Genome Coverage                   :: 20.4x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000497445.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 03:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,098
            CDSs (total)                      :: 9,013
            Genes (coding)                    :: 8,393
            CDSs (with protein)               :: 8,393
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 6 (16S, 23S)
            complete rRNAs                    :: 6, 6 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 620
            CDSs (without protein)            :: 620
            Pseudo Genes (ambiguous residues) :: 0 of 620
            Pseudo Genes (frameshifted)       :: 227 of 620
            Pseudo Genes (incomplete)         :: 534 of 620
            Pseudo Genes (internal stop)      :: 54 of 620
            Pseudo Genes (multiple problems)  :: 178 of 620
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:03:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7485236
            Orig. end                         :: 7495641
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CM002285.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="37"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(744..760)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.9"
                     /tool="antismash"
     gene            768..1376
                     /locus_tag="M878_RS81985"
                     /old_locus_tag="M878_34945"
     CDS             768..1376
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="M878_RS81985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_34945"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_023551670.1"
                     /transl_table=11
                     /translation="MDVELPDIPFPLRTYGPDGHWSYEDGVLTGWAGPRQDRFVTPTGE
                     ALDPASDAPRLLGAPEGDFQLIARVTVGFGAAFDAGVLYVHVGDRAWAKLCLEYSPDVP
                     TVCTVVTRGHSDDCNSFTVEGSSVWLRISRTGRAFAFHASRDGERWTFVRLFTLGDEKE
                     TGAALVGFMTQSPLGEGCVVTYDRLRFRTAWPADLRDGS"
     gene            1543..2046
                     /locus_tag="M878_RS81990"
                     /old_locus_tag="M878_34950"
     CDS             1543..2046
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019329308.1"
                     /locus_tag="M878_RS81990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_34950"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_023551671.1"
                     /transl_table=11
                     /translation="MISERVTGALVIRTALPAEAETIADLHVRARSTYYPDGLPQADFD
                     WAEAWRGAIERPDGHVLCAVAQGRIAGIASFRTPQGAPADTVKLFQFHVDPGHWRAGVG
                     TALHAACVEEWRADGKRTAVLDVHTDNRRAQGFYARQGWIPDPENPPAEGDHHLFLCFS
                     VPGQ"
     misc_feature    2056..2067
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            2139..2861
                     /locus_tag="M878_RS81995"
                     /old_locus_tag="M878_34955"
     CDS             2139..2861
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381151.1"
                     /locus_tag="M878_RS81995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_34955"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_023551672.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDDVEVVHRSFELDP
                     GRAKGDVQPVITMLTRKYGMSEAQAEAGEDNLGAQAAAEGLDYRTRGRDHGNTFDMHRL
                     LHFAKERGRQDELIQLLYRANFAEERSVFTEGDERLVELAAEAGLDAEAARAVLADPSR
                     YADEVRADEREAAQLGATGVPFFVLDRKYGVSGAQPAEVFTRALAQAWGEHSQLRLVAQ
                     GEDAGTCGPDGCAVPQQG"
     gene            2957..4018
                     /locus_tag="M878_RS82000"
                     /old_locus_tag="M878_34960"
     CDS             2957..4018
                     /NRPS_PKS="Domain: Aminotran_5 (44-347). E-value: 2.2e-25.
                     Score: 81.3. Matches aSDomain:
                     nrpspksdomains_M878_RS82000_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671936.1"
                     /locus_tag="M878_RS82000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_34960"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_023551673.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.5e-19, bitscore:
                     68.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METTETFDSLVRAEFHPQTSYLNTASNGLLPARAVAALHEAALMR
                     AEGRPLLPLYEDVEICREQYARLAGVPATRVAAGASVAAHTGVLALSLPPGAEVLTAEE
                     DFTSVLNPFHVRGDLKVRSVPLERIAESVRPGTALVAVSAVQSADGRLADLPAVREAAR
                     AHGARTYVDLSQCVGWLPVDASQYDFTATVSFKWLLGPHGAAFLTVPEDFGGLTPLLAG
                     WVAAEAPWDSCYGPVTELAHSARRFDLTHALFTYAGLRGSLELLEEIGASAVHEHAVRL
                     ADRFRGGLTALGHEPVPAPGSAIVSVPGLGARQQELSRAGIEVSNRAGNLRAAFHLYNT
                     ETDVDRLLNALAG"
     aSDomain        3089..3997
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_M878_RS82000_Aminotran_5.1"
                     /evalue="2.20E-25"
                     /label="M878_RS82000_Aminotran_5.1"
                     /locus_tag="M878_RS82000"
                     /protein_end="347"
                     /protein_start="44"
                     /score="81.3"
                     /tool="antismash"
                     /translation="RAEGRPLLPLYEDVEICREQYARLAGVPATRVAAGASVAAHTGVL
                     ALSLPPGAEVLTAEEDFTSVLNPFHVRGDLKVRSVPLERIAESVRPGTALVAVSAVQSA
                     DGRLADLPAVREAARAHGARTYVDLSQCVGWLPVDASQYDFTATVSFKWLLGPHGAAFL
                     TVPEDFGGLTPLLAGWVAAEAPWDSCYGPVTELAHSARRFDLTHALFTYAGLRGSLELL
                     EEIGASAVHEHAVRLADRFRGGLTALGHEPVPAPGSAIVSVPGLGARQQELSRAGIEVS
                     NRAGNLRAAFHLYNTETDVDRL"
     gene            complement(4101..4994)
                     /gene="thpD"
                     /locus_tag="M878_RS82005"
                     /old_locus_tag="M878_34965"
     CDS             complement(4101..4994)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671935.1"
                     /locus_tag="M878_RS82005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_34965"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_023551674.1"
                     /sec_met_domain="PhyH (E-value: 7.5e-40, bitscore: 136.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTADVTDLYPTRGTTEVTVPRQDPVVWGSPDTPGPIPTADLQGY
                     DRDGFLAIEQLITPDEVEVYRRELERLVTDPAIRADERSIVEPKSKEIRSVFEVHRISE
                     VFANLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENHDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDEALT
                     SLASAHGIRLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENKAVDPFAAP
                     VRRPDFIGARDFTPVR"
     gene            complement(5001..5405)
                     /locus_tag="M878_RS82010"
                     /old_locus_tag="M878_34970"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="M878_RS82010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_34970"
                     /product="ectoine synthase"
                     /protein_id="WP_023551675.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.5e-53, bitscore:
                     179.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETILY
                     AGTETSMWYANHIEAVVCTQGEAELTDRETGETYIITPGTMYLLNGHERHTLRVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            complement(5448..6719)
                     /gene="ectB"
                     /locus_tag="M878_RS82015"
                     /old_locus_tag="M878_34975"
     CDS             complement(5448..6719)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 2e-72.
                     Score: 236.0. Matches aSDomain:
                     nrpspksdomains_M878_RS82015_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="M878_RS82015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_34975"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_023551676.1"
                     /sec_met_domain="Aminotran_3 (E-value: 6.9e-77, bitscore:
                     257.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSRMYDEDGHVY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLARDGVTHGLDMSTTAKRSFLQAFQDLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     DWLRALKDLCERQDMLLIVDDIQMGCGRTGAFFSFEEAGIAPDIVTVSKSISGYGLPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTATAALETYWADGSAMEKQTRARGEQVEQAL
                     ISITEENLADVKEYRGRGLVWGMEFHDKDRASRIARRAFELGLLIETSGPEGEVVKLLP
                     ALTITPDELDEGLRVLARAVRETA"
     aSDomain        complement(5622..6635)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_M878_RS82015_Aminotran_3.1"
                     /evalue="2.00E-72"
                     /label="M878_RS82015_Aminotran_3.1"
                     /locus_tag="M878_RS82015"
                     /protein_end="366"
                     /protein_start="28"
                     /score="236.0"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHVYLDFFAGAGSLNYGHNNPVLKRALIDYLA
                     RDGVTHGLDMSTTAKRSFLQAFQDLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPDWLRALKDLCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIAPDIVTVSKSISGYGLPMSLCLFKPELDVWEPGEHNGTFRGNNPAFV
                     TATAALETYWADGSAMEKQTRARGEQVEQALISITEENLADVKEYRGRGLVWGMEFH"
     gene            complement(6733..7251)
                     /gene="ectA"
                     /locus_tag="M878_RS82020"
                     /old_locus_tag="M878_34980"
     CDS             complement(6733..7251)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671932.1"
                     /locus_tag="M878_RS82020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_34980"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_023551677.1"
                     /transl_table=11
                     /translation="MTAAPADLLIDQPAVTDGAALWRLAKDSKTLDLNSSYSYLLWCRD
                     FAGTSAVARGADGEPVGFVTGYVRPERPRTLLVWQVAVDSGHRGLGIAAALLDALTARL
                     TAERGITDVETTITPGNTASERLFTSFADRHGARLERAVLFAAELFPDGPHDPEVLYRI
                     GPLTPFTSH"
     misc_feature    complement(7370..7385)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            complement(7518..8057)
                     /locus_tag="M878_RS82025"
                     /old_locus_tag="M878_34985"
     CDS             complement(7518..8057)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010355555.1"
                     /locus_tag="M878_RS82025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_34985"
                     /product="DinB family protein"
                     /protein_id="WP_023551678.1"
                     /transl_table=11
                     /translation="MTDDTTPQPPRTLPDGRPVPPMTGPERAMLEGWLDFHRATLELKC
                     RGLDDAQVRLTSAEPSSLTLLGLVQHLAEVERNWFQRVLAGLDVPRAFEERTGCGLDPE
                     RGLEDALAVWRAEITRGRELLAERDLADTGRIVDEPMTGVEVSVRWVLMHLIEEYARHN
                     GHADILRERIDGTTGT"
     gene            complement(8115..9158)
                     /locus_tag="M878_RS82030"
                     /old_locus_tag="M878_34990"
     CDS             complement(8115..9158)
                     /NRPS_PKS="Domain: Aminotran_5 (74-308). E-value: 2e-28.
                     Score: 91.3. Matches aSDomain:
                     nrpspksdomains_M878_RS82030_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037833.1"
                     /locus_tag="M878_RS82030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_34990"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_023551679.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.3e-21, bitscore:
                     75.2, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSHPFLDLPPLSAERFAAIEDGIARLLDTRQDVLITQGEALLPLE
                     GAIRAAAGPGTVALNVITGPYGQTFGNWLRDSGATVYDISVPFHTAVTAAQVREAFAEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEAVREHGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAISVSERAWTRMAANPHAPRRSYLSLLDWKERWIDAGRVVLPHAP
                     AQLEMLALEACLERIESVGLDAVMDRHRRAAQATRAGAVSVGRGLEPYVYEACDAAPVA
                     TTLRVPSGVVASELVARALASDPALPLAAGGGALAKEMIRVNHYGADASTETVRRCLAA
                     IGEALGV"
     aSDomain        complement(8235..8936)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_M878_RS82030_Aminotran_5.1"
                     /evalue="2.00E-28"
                     /label="M878_RS82030_Aminotran_5.1"
                     /locus_tag="M878_RS82030"
                     /protein_end="308"
                     /protein_start="74"
                     /score="91.3"
                     /tool="antismash"
                     /translation="RDSGATVYDISVPFHTAVTAAQVREAFAEHPEIDFVSLVHAEAAT
                     GNTNPVAEIGEAVREHGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSA
                     ISVSERAWTRMAANPHAPRRSYLSLLDWKERWIDAGRVVLPHAPAQLEMLALEACLERI
                     ESVGLDAVMDRHRRAAQATRAGAVSVGRGLEPYVYEACDAAPVATTLRVPSGVVASELV
                     ARALASDPALPL"
     gene            9312..10154
                     /locus_tag="M878_RS82035"
                     /old_locus_tag="M878_34995"
     CDS             9312..10154
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121392.1"
                     /locus_tag="M878_RS82035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_34995"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_023551680.1"
                     /transl_table=11
                     /translation="MKTLGRRTRLLAATTATAGLVLVAGCSSSGSGSGPTTVKGVHLAK
                     AGQLTTCTHLPYPPFQSEINGKVQGFDVSLIDLVAKDLGVKQQILDTPFENFKTGAFLN
                     SGQCDLAAAGMTITAERKKNVDFSDPYFEATQAVLVDRSSGVNSLADVKAKGKKLGAQA
                     QTTGEDYARSKGFDPVSFASSDAVLNGLRTGQVQAVIIDYPVVQGWLKDKANADKFKVV
                     DNLKTGEQYGFTVKKGNKALLDAVNKALKGAKADGTYKKIYEQWIGPYNASVAAPAAS"
ORIGIN
        1 ggtgtcgggc tcgccgcgtc catgacgctc ggcgtcggac agttctgcgt caagcccggg
       61 ctggtgctgg tgccgtccgg cgcgggcggc gaccggctgg tgaagtccct caccgacgcg
      121 gtcagtgaca ccgaggcggg tgttctgctc gaccaccgga tgcgggacaa cttcgtcgcc
      181 ggcgtccggg agcgggcccg tctggaggag gtcgactcgc ccgtcacacc cggcgcgggc
      241 ggcgagcaca cggtcagtgc gggggtcctc tcggtgccgg cggagaaact ggccgaggag
      301 ggggcgtacg acctgctgct ggaggagtgc ttcgggccgg tcaccgtagt ggcccgctat
      361 tcctccgagg gggaggcggc ggcggtgctg tcgcgtctgc cgggcaacct gacggcaacc
      421 gttcagctgt cggccgagga ggccgccggg gaggggcggg gacccgggct tctcgccgag
      481 ctgactccgc tggcggggcg cgtggtcgtg aacgcgtggc ccacgggggt cgcggtggcg
      541 cccgcgcagc atcacggggg gccgtacccg gcgacgacct ccacgtcgac ttccgtgggc
      601 ggtacggcca ttgagcggtg gctgcggccg gtggcgtacc agaacgcgcc cgccgcgctg
      661 ttgccgaagg aacttcgcga cgagaatccg ctggggcttc cccggaggtt caacggggtc
      721 ttggagcggt agcccgggct cacatcttct cttgcgacaa tcggaccatg gacgtggaac
      781 tccccgacat tcccttccct ctccgtacct acgggcccga tggccactgg tcgtacgagg
      841 acggtgtgct caccgggtgg gccgggcccc ggcaggaccg tttcgtcacg cctaccgggg
      901 aagccctgga tcccgcctcg gacgcgccgc ggttgctggg ggcgccggag ggggacttcc
      961 agctgatcgc gcgggtcacc gtggggttcg gcgccgcctt cgacgcgggc gtgctgtacg
     1021 tccatgtcgg tgaccgggcc tgggccaagt tgtgtctgga gtactccccg gacgtgccca
     1081 ccgtctgcac ggtggtcacc cgggggcact ccgacgactg caactccttc acggtggaag
     1141 ggagttcggt gtggctgcgg atcagccgga ccgggcgggc cttcgccttc cacgcctcgc
     1201 gggacggcga gcggtggacc ttcgtccggc tgttcaccct cggcgacgag aaggagaccg
     1261 gcgccgcgct ggtcggcttc atgacccagt cgccgctggg ggaggggtgc gtggtcacct
     1321 acgatcgcct ccggttcagg accgcttggc cggccgacct ccgagacgga agctgagcgc
     1381 gaacgaacac tccggccgcc agggccgggg gcgtaccggc gctgacgtac gccggcacga
     1441 gcagaccggc gacggccagt ggctgtctga cacgactgtg gcgccccgga ggcgtaaaga
     1501 tcgacacgga accacaggcg gtgccgtcac gtcctccgtt gcatgatcag tgaacgtgtg
     1561 accggcgccc tggtgatccg caccgccctg cccgccgaag ccgagaccat cgccgatctg
     1621 catgtccggg cccgctcgac gtactacccg gacgggctcc cgcaggccga cttcgactgg
     1681 gccgaggcct ggcgcggcgc catcgagcgc ccggacggcc atgtgctgtg cgccgtcgcc
     1741 cagggccgga tcgccggcat cgcctccttc cgcactccgc agggcgcacc cgccgacacg
     1801 gtcaagctgt tccagttcca cgtcgacccc ggccactggc gcgccggtgt cggaacggcc
     1861 ctgcacgcgg cctgcgtgga ggagtggcgg gccgacggca agcgcacggc cgtgctcgac
     1921 gtgcatacgg acaaccggcg tgcccagggc ttctacgccc gccagggctg gatcccggat
     1981 ccggagaacc cgcccgccga gggcgaccac cacctcttcc tgtgcttctc cgtgcccggc
     2041 cagtgagccg tgcccgggaa tgaacgcggc tgtttgaacg ttcacgtacc ggcaggagag
     2101 agcctccgcg cccgtacgac ctggagagag ccgaagacat gcgcgtcgag atctggagcg
     2161 acatcgcctg cccgtggtgc tacgtgggca aggcccgctt cgagaaggcg ctggctgcct
     2221 tcccgcaccg cgacgacgtc gaggtggtgc accgctcctt cgagctggac ccgggccggg
     2281 ccaagggtga cgtccagccg gtgatcacca tgctgaccag gaagtacggc atgagcgagg
     2341 cgcaggccga ggccggcgag gacaacctgg gcgcccaggc cgccgccgag ggcctggact
     2401 accgcacccg gggccgcgac cacggcaata ccttcgacat gcaccgcctg ctgcacttcg
     2461 ccaaggagcg gggccggcag gacgagctga tccagctcct gtaccgggcg aacttcgccg
     2521 aggagcggtc cgtcttcacc gagggcgacg agcgcctggt ggagctggcc gccgaggccg
     2581 gactggacgc ggaggccgcc cgcgcggtgc tcgccgaccc gtcccgctac gccgacgagg
     2641 tccgcgccga cgagcgcgag gccgcccagc tcggcgcgac cggcgtgccg ttcttcgtcc
     2701 tcgaccggaa gtacggcgtc tccggcgccc agcccgccga ggtcttcacc cgggcgctgg
     2761 cccaggcctg gggcgagcat tcccagctgc gactggttgc gcagggggag gacgccggga
     2821 cctgcggtcc cgacgggtgc gcggtaccgc agcagggctg aaaccgcagg ccggagcggt
     2881 cctataagcg gtcctaaggg gaaccacgta aaaatcagca atggacgggt agttccctgg
     2941 gacgcagagt ggtcccatgg agaccacgga gacgttcgac agcctcgtcc gcgccgagtt
     3001 ccacccgcag accagctacc tgaacaccgc gagcaacggc ctgctgcccg cccgcgccgt
     3061 cgcagccctg cacgaggcgg cgctgatgcg ggcggagggc cgcccgctgc tcccgctcta
     3121 cgaggacgtg gagatctgcc gggagcagta cgcccggctg gccggtgtcc cggccactcg
     3181 ggtcgcggcg ggcgcctcgg tcgccgcgca caccggcgtg ctcgccctct cgctgccccc
     3241 gggtgccgaa gtcctcaccg cggaggagga cttcacctcc gtactgaacc cgttccacgt
     3301 ccgcggcgac ctcaaggtgc gctcggtccc gctggagcgg atcgccgagt ccgtccgccc
     3361 gggcaccgcg ctcgtcgcgg tcagcgccgt ccagtccgcc gacgggcggc tcgccgacct
     3421 gcccgcggtg cgcgaggccg cccgcgcgca cggcgcccgc acctacgtcg acctctccca
     3481 gtgcgtcggc tggctcccgg tggacgcgtc ccagtacgac ttcaccgcca ccgtctcctt
     3541 caagtggctg ctcggcccgc acggcgcggc cttcctcacc gtccccgagg acttcggcgg
     3601 gctcactccg ctgctcgcgg gctgggtcgc ggccgaggca ccgtgggaca gctgctacgg
     3661 cccggtgacc gaactcgccc actccgcacg gcggttcgac ctcacccacg ccctgttcac
     3721 ctacgccgga ctgcgcggct ccctcgaact gctggaggag atcggggcgt ccgcggttca
     3781 cgagcacgcc gtgcgcctcg ccgaccgctt ccgcggcgga ctcaccgccc tcggccatga
     3841 gccggtgccc gccccgggct cggcgatcgt ctccgtcccc ggactcggcg cccgccagca
     3901 ggagttgagc cgcgccggca tcgaggtctc caaccgcgcc ggcaacctcc gtgccgcctt
     3961 ccacctctac aacaccgaga cggacgtgga ccgtctgctg aacgccctgg ccggctgagc
     4021 ccgctcatgt gtgacacacg ccaggggcct ccaccacggt ggtggaggcc cctggcgtgt
     4081 gggttcagtg acccatgggt tcagcggacg ggagtgaagt ccctcgcccc gatgaagtcc
     4141 ggccggcgca ccggcgccgc gaacggatcg accgccttgt tctccacgct gttgaacacg
     4201 atgaagacgt tgctgcgcgg gaacggcgtg atgttgtcgc cggagccgtg catgcagttg
     4261 cagtcgaacc aggtcgccga gccggccttg ccggtgaaca gccggatgcc gtgcgcgctc
     4321 gccagcgagg tcagcgcctc gtcggacggc gtgcccgcgt cctgcatctg cagcgacttc
     4381 ttgtagttgt ccttgggcgt ctcaccggca cagcccagga acgtcttgtg cgagcccggc
     4441 atgatcatca ggccgccgtt ggtgtcgtgg ttctccgtca gggcgatgga gacggacacc
     4501 gtgcgcatgt tcggcagacc gtcctcggcg tgccaggtct cgaagtcgga gtgccagtag
     4561 aagccgctcg cgccgaagcc cggcttgacg ttgatccggg actggtggac gtacacgtcc
     4621 gagccgagga tctgccgggc ccggccgacc acccgctcat cgcgcaccag gttcgcgaac
     4681 acctcgctga tccggtgcac ctcgaagacc gagcggatct ccttcgactt cggctcgacg
     4741 atggagcgct cgtcggcgcg gatcgccggg tcggtgacca gccgctccag ctcgcgccgg
     4801 tagacctcga cctcgtccgg ggtgatcagc tgctcgatgg cgaggaagcc gtcacggtcg
     4861 tacccctgca gatcggccgt ggggatcggg cccggcgtgt ccggggaacc ccagacgacc
     4921 gggtcctgac gcgggacggt cacctcggtg gtgccgcggg tcgggtagag atcggtgacg
     4981 tcggcagtgg tcatgggttc tcacacctcc tcgggctcgg tgagcagggg gtagacgccg
     5041 ttctcgtcgt ggtcctcccg gccggtcacg ggcgggttga acacgcagat gcagtggaag
     5101 tcctccttga cgcgcagcgt gtgccgctcg tgcccgttga ggaggtacat ggtgccgggc
     5161 gtgatgatgt acgtctcccc ggtctcgcgg tcggtgagtt cggcctcacc ctgggtgcag
     5221 acgacggcct cgatgtgatt ggcgtaccac atcgacgtct cggtcccggc atagaggatg
     5281 gtctcgtgca gggagaagcc gacccgctcc ttggccagga cgatccgctt gctctcccag
     5341 gtgccggact tcgccttgac gtgccggtcg gtgccttcga tgtccttgaa cgatcggacg
     5401 atcacggtgg tgctcttcct cctggtcggt cgtgccgtca gtacggttca ggcggtttcc
     5461 cggacggcac gggcgaggac gcgcaggccc tcgtccagct cgtcgggggt gatggtgagg
     5521 gcgggcagca gcttgacgac ctcgccctcc ggtccggacg tctcgatgag cagcccgagt
     5581 tcgaaggccc gccgggcgat ccgcgaggcc cggtccttgt cgtggaactc catgccccac
     5641 accagaccgc ggccgcggta ctccttcaca tcggcgaggt tctcctcggt gatggagatc
     5701 agcgcctgct cgacctgctc accgcgggcg cgggtctgct tctccatggc cgagccgtcg
     5761 gcccagtacg tctccagcgc cgcggtggcg gtgacgaacg cggggttgtt gccccggaag
     5821 gtgccgttgt gctcgcccgg ctcccacacg tccagctcgg gcttgaacag gcacagcgac
     5881 atcggcagcc cgtacccgct gatcgacttg gacaccgtga cgatgtccgg cgcgatcccc
     5941 gcctcctcga aggagaagaa ggcgccggta cggccgcagc ccatctggat gtcgtcgacg
     6001 atcagcagca tgtcctgccg ctcgcacaga tccttcagcg cgcgcagcca gtcgggccgg
     6061 gcgacgttga tgccgccctc gccctgcacg gtctccacga tcaccgcggc gggcttgttc
     6121 agaccggagc cctggtcctc caggaggcgc tcgaaccaca ggaagtcagg gaccgtgccg
     6181 tcgaagtagt tgtcgaacgg catcggcgtg ccgtgcacca gcgggatccc ggcgccggcc
     6241 cgcttgaagg cgttgccggt cacggcgagc gagcccagcg acatcccgtg gaaggcattg
     6301 gtgaacgaca cgatggcctc gcggcccttc accttccggg ccagcttcag cgccgactcc
     6361 acggcgttgg tgccggtcgg ccccgggaac atgaccttgt acggcagatc gcgcgggcgc
     6421 agcaccagat cctggaaggc ctgcaggaag gaccgcttgg ccgtggtcga catgtccagg
     6481 ccgtgggtca ccccgtcccg cgccagatag tcgatcaacg cccgtttgag tacggggttg
     6541 ttgtggccgt agttgagtga gccggcaccg gcgaagaagt cgaggtacac atgaccgtcc
     6601 tcgtcgtaca tccggctgcc ctgcgcgcgg tcgaagaccg tgggccagcc gcggcagtag
     6661 ctgcgcacct cggactcgag ggtctcgaag acgctgaggt cgggctgggt gatggtcacg
     6721 gtgaatcgct cctcaatgcg aggtgaaggg ggtcaacggg ccgatgcggt aaaggacttc
     6781 ggggtcgtgc ggaccgtcgg ggaacagctc ggcggcgaac agcaccgcac gctccaggcg
     6841 tgccccgtgc cggtcggcga acgaggtgaa cagccgctcg gaggcggtgt tgccgggggt
     6901 gatggtcgtc tccacgtcgg tgatgccgcg ctcggcggtg agccgtgcgg tcagcgcgtc
     6961 cagcagcgcg gcggcgatgc cgaggccccg gtggccggag tcgacggcca cctgccagac
     7021 gagcagggtg cgggggcgct ccggccgcac atagccggtg acgaatccga ccggctcccc
     7081 gtccgcaccg cgcgccacgg ccgaggtgcc ggcgaagtcc cggcaccaca gcagatagct
     7141 gtacgaggag ttcaagtcga gggttttcga gtccttggcg agacgccaga gcgcggctcc
     7201 gtcggtcacc gccggctggt cgatcagaag gtctgcgggt gcggcagtca tgcggcacga
     7261 atttagcgag gtgaattgag aaatgcacgg gcgccctcgc tgtgccgcac agaggacacc
     7321 gtgaaatgtc cgaattaccc catggcaaac ctggacaaat caactctgat gtggagtgcg
     7381 tcacagtaag tggatcgtca aggcctcgcc agaaacccgc tggaaaactc ccccgtttag
     7441 cctcgcgaaa agcgggcaga agaaaacggg aagctatcgt ccagggaatt agcgggaatt
     7501 gtgaagctaa attgaattca ggtaccggtg gttccgtcga ttcgttcacg cagaatgtcg
     7561 gcgtggccgt tgtgccgggc gtactcctcg atgaggtgca tcagcaccca gcgcacactg
     7621 acctcgacac cggtcatcgg ctcgtcgacg atccgtccgg tgtccgccag atcccgctcg
     7681 gccagcagct cccggccgcg cgtgatctcc gcccgccata cggccagcgc gtcctccagc
     7741 ccacgctccg gatcgagccc gcaccccgtc ctctcctcga acgcccgcgg cacgtccagc
     7801 ccggcgagca cccgctggaa ccagttccgc tcgacctcgg cgagatgctg gacgagcccc
     7861 agcagggtca gcgaagacgg ctccgccgag gtgagccgga cctgcgcgtc gtccagtccc
     7921 cggcacttca gttccagggt ggcccggtgg aagtccagcc agccttccag catggcgcgc
     7981 tcgggcccgg tcatcggggg gacggggcgg ccgtcgggga gggtgcgcgg gggctggggt
     8041 gtggtgtcgt cggtcatgca gggaggatgg cacgggccac tgacaacaac gccgccgacc
     8101 atcgcgacca ggtgtcaaac gccgagtgcc tccccgatcg ccgccagaca cctccgcacc
     8161 gtctccgtcg aagcgtccgc cccgtagtgg ttgacccgga tcatctcctt ggccaacgca
     8221 cccccacccg cggccagcgg cagcgcggga tcactcgcca gtgcccgagc caccagctcc
     8281 gacgccacca cccccgacgg cacccgcagt gtcgtagcca ccggcgcggc atcgcaagct
     8341 tcgtacacat acggctcaag cccccggccc acggacaccg caccggcccg cgtggcctgt
     8401 gcggcgcgcc gatgccggtc catcaccgcg tccagcccca ccgactcgat ccgctccaga
     8461 cacgcctcca gcgccagcat ctccaactgg gccggagcgt gcggcagcac cacgcggccg
     8521 gcgtcgatcc agcgctcctt ccagtccagc agggacagat aggagcggcg cggcgcgtgc
     8581 gggttcgccg ccatccgggt ccaggcccgc tcgctcaccg agatcgccga cacacccgcc
     8641 ggtccgccca tcgccttctg cgccccgatc acgcacaggt ccacgcccca cgcgtccggc
     8701 agtaccggct ccgcgcccac cgaggcgacc gcgtccaggt agaagagcgc accgtgctcc
     8761 cgtaccgcct cgccgatctc cgcgaccggg ttggtgttgc cggtcgccgc ctccgcgtgc
     8821 accagcgaca cgaagtcgat ctccgggtgc tcggcgaagg cctcccggac ctgcgcggcg
     8881 gtcaccgccg tatggaaggg gacggagatg tcgtacacgg tcgcaccgga atcgcgcagc
     8941 cagttgccga aggtctggcc gtacggtccc gtgatcacgt tcagggccac cgtgcccggg
     9001 ccggccgccg cgcggattgc gccctccagc ggcagcagcg cctcaccctg ggtgatcagc
     9061 acgtcctggc gggtgtccag cagtcgggcg atcccgtcct cgatggcggc gaagcgctcg
     9121 gcactcagcg ggggcagatc gaggaacggg tggctcacgg cggtgctctc tcttcgttca
     9181 cggcggtcgg gactcccttg atcttaggtc caatggcccc gaaaccatcg gtttggtttg
     9241 agagactcaa actcttcctt ataatcggaa cccatagttc ccgcacaagg tcccccagac
     9301 ggagatcccc catgaaaacg ctcggacgcc ggacccgcct tctggcagcc accaccgcga
     9361 cggccgggct cgtgctcgtg gccggttgct cctccagcgg ctcggggagc ggcccgacga
     9421 ccgtcaaggg ggtccacctc gccaaggccg gtcagctgac cacctgcacc cacctgccgt
     9481 acccgccgtt ccagtcggag atcaacggca aggtgcaggg cttcgacgtt tccctcatcg
     9541 acctcgtcgc caaggacctc ggcgtcaagc agcagatcct cgacacgccg ttcgagaact
     9601 tcaagaccgg tgccttcctg aactccggcc agtgcgacct ggccgccgcc ggcatgacga
     9661 tcaccgccga gcgcaagaag aacgtcgact tctccgaccc gtacttcgag gccacccagg
     9721 ccgtcctggt cgacaggagc agcggcgtca actcgctcgc ggacgtcaag gccaagggca
     9781 agaagctcgg cgcccaggcg cagaccaccg gcgaggacta cgccaggagc aagggcttcg
     9841 acccggtctc cttcgcctcc tccgacgcgg tcctcaacgg cctgcgcacc ggacaggtcc
     9901 aggccgtcat catcgactac ccggtcgtcc agggctggct gaaggacaag gccaacgccg
     9961 acaagttcaa ggtcgtcgac aacctcaaga ccggcgagca gtacggcttc acggtcaaga
    10021 agggcaacaa ggccctgctc gacgccgtca acaaggccct caagggcgcc aaggccgacg
    10081 gcacgtacaa gaagatctac gagcagtgga tcggccccta caacgcctcc gtcgccgccc
    10141 cggccgcctc ctgaccccat gaccgacacg gacacacata tccagccgaa gaagaccggg
    10201 ctgacccgtc ggcagaagcg ccggctctcg cgtgcggcgc agtacgccgt cttcgtcgcc
    10261 gcggtgatcg ccttcgcggt caccgcggac tggggccggc tgcagaacca gttcgcgcag
    10321 gcccacatcg cgaggcagct gttcccggac ctcatcacgg tggccctcaa gaacaccgtg
    10381 ctgtacacgc tgtccggctt tgtcg
//
