LOCUS       NZ_CM002285            10414 bp    DNA     linear   CON 29-APR-2024
DEFINITION  Streptomyces roseochromogenus subsp. oscitans DS 12.976 chromosome,
            whole genome shotgun sequence.
ACCESSION   NZ_CM002285
VERSION     NZ_CM002285.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseochromogenus subsp. oscitans DS 12.976
  ORGANISM  Streptomyces roseochromogenus subsp. oscitans DS 12.976
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9663240)
  AUTHORS   Ruckert,C., Kalinowski,J., Heide,L. and Apel,A.K.
  TITLE     Draft Genome Sequence of Streptomyces roseochromogenes subsp.
            oscitans DS 12.976, Producer of the Aminocoumarin Antibiotic
            Clorobiocin
  JOURNAL   Genome Announc 2 (1), e01147-13 (2014)
   PUBMED   24407645
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663240)
  AUTHORS   Ruckert,C., Szczepanowski,R., Heide,L., Kalinowski,J. and Apel,A.K.
  TITLE     Draft genome sequence of Streptomyces roseochromogenes subsp.
            oscitans DS 12.976
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 9663240)
  AUTHORS   Ruckert,C., Szczepanowski,R., Heide,L., Kalinowski,J. and Apel,A.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2013) CeBiTec, Bielefeld University,
            Universitaetsstr. 27, Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.5.3
            Assembly Name                     :: S.roseochromogenes_v1
            Genome Coverage                   :: 20.4x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000497445.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 03:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,098
            CDSs (total)                      :: 9,013
            Genes (coding)                    :: 8,393
            CDSs (with protein)               :: 8,393
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 6 (16S, 23S)
            complete rRNAs                    :: 6, 6 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 620
            CDSs (without protein)            :: 620
            Pseudo Genes (ambiguous residues) :: 0 of 620
            Pseudo Genes (frameshifted)       :: 227 of 620
            Pseudo Genes (incomplete)         :: 534 of 620
            Pseudo Genes (internal stop)      :: 54 of 620
            Pseudo Genes (multiple problems)  :: 178 of 620
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:03:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6345758
            Orig. end                         :: 6356172
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CM002285.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10414
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="32"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10414
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10414
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5414](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5414
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            <84..1016
                     /locus_tag="M878_RS92450"
                     /old_locus_tag="M878_29560"
     CDS             <84..1016
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /locus_tag="M878_RS92450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_29560"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_023550639.1"
                     /transl_table=11
                     /translation="SEEADVCAADDTPDLWCPACAELGKPLRVVVEGRYRLVRCRVCRT
                     QYFRADPALAGCEGEAPFNEYWEQYKFGLYASDEVRRDYEERYEAILDEAERLTGPIDT
                     VLDVGCGIGNFVSFAQARCGRAFGIDVDADAVAAARSRGLRVALSEDLDALLPDAEADA
                     VTLWDVIEHLYDPLPAVSRALGKLRPGGALIFETPDASFPVRPVLRAVHTASRGRVDLT
                     RHLYDWEHKIYFTAAGMRAILSRLDCEVISVQRPASPRAEMRENFTRHADASWQSRALA
                     RAWPRLESAARRVGRGSKLVVIARDAGCG"
     gene            complement(1060..2235)
                     /locus_tag="M878_RS77080"
                     /old_locus_tag="M878_29565"
     CDS             complement(1060..2235)
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018529506.1"
                     /locus_tag="M878_RS77080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_29565"
                     /product="glycosyl hydrolase"
                     /protein_id="WP_023550640.1"
                     /transl_table=11
                     /translation="MASERLRSLRRVTFLAAGVFAAASVLPSPVIKSGPTANPAAQHCA
                     AGFAAPVPAGCAANGATGPSSGEPRPPAFGAYLSSGPLGVLRIRGFSDWLDHTEVRVGH
                     TYLPGDKWSNIEGTPGFLGNWAQWRRAKDDRILVVNVPLTERDEGHVPDTEVQQVLRRG
                     AAGEFDQHFRTLAEHLVGLGVPDTVLVLGWEMNGVTYTHRCGPDPDAWKTYWRRIVTVM
                     RSVPGQHFAFDFAPDRGADAVPWPQCYPGDDVVDVIGMDSYDQPAGISFDQQVSEPYGL
                     QDQVDFAKAHKKPISYPEWGLFRNGDNATYMLRMLAWIDEHKPLYNTITDYCPHGVWLC
                     PANPRASALYRAALSGRRGPDQDPNPPVPAAPEPANEPHPLPNPLPARASG"
     gene            complement(2406..3161)
                     /locus_tag="M878_RS77085"
                     /old_locus_tag="M878_29570"
     CDS             complement(2406..3161)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="M878_RS77085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="M878_29570"
                     /product="hypothetical protein"
                     /protein_id="WP_023550641.1"
                     /transl_table=11
                     /translation="MRHTLTKGLVVAAAATSVLTVYSPWAFADSGPFTSDDAYSSYSDD
                     VGDSDFPGAASAMRAMERAESATEMAQNLASEFSSSYSPGWSTGHVTKSHRESSSEDYG
                     DDESSEHSSHESYGDDEKPSHKPSHESYGDDEKPSHKPSHESYGDDKSNYGDDESNYGD
                     DESSYGNEEKPPHHHHEPKPVHHHHHHHMPPERPEKPHHRHHHHHHHHHRPMAHTGTEG
                     TIAAVAASGALLVAGTVLYRRGRKTTAQQ"
     misc_feature    complement(3310..3328)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 19.72"
                     /tool="antismash"
     gene            complement(<3732..>3871)
                     /locus_tag="M878_RS77090"
                     /old_locus_tag="M878_29580"
                     /pseudo=""
     CDS             complement(<3732..3871)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019356319.1"
                     /locus_tag="M878_RS77090"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="M878_29580"
                     /product="AMP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MVAVMSRTRYEWTVLDFALWPPVARASPSAPPLRPSRWSGSCATP
                     A"
     gene            4213..4761
                     /locus_tag="M878_RS77095"
                     /old_locus_tag="M878_29585"
     CDS             4213..4761
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007447554.1"
                     /locus_tag="M878_RS77095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_29585"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_031225974.1"
                     /transl_table=11
                     /translation="MLLVLTGAAGLLASWVITIDKLKLLQNPNFTPGCSLNPVVSCGSV
                     MKSPQASVFGFPNPMLGLVAYGIVICVGISLLAGAAFPRWYWLIFDAGCLFGVGFVSWL
                     QFESLYRINALCLWCALAWVATILMFWYVTSFAVRHRFLPAPGPLRAFFAEFTWVLPLL
                     HIAVVGMLILTRWWDFWTS"
     gene            5001..5414
                     /locus_tag="M878_RS77100"
                     /old_locus_tag="M878_29590"
     CDS             5001..5414
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672801.1"
                     /locus_tag="M878_RS77100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_29590"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_342452733.1"
                     /sec_met_domain="melC (E-value: 1.9e-41, bitscore: 140.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRRLFSSALVVALAPVTAACARRAEHAPDDGRRATAFDETYRGR
                     HIRGVEVHPAERAAVAGAQWKVTIDGHPLHLMRRADGSWLSMIDHYRSYPTALAAARGA
                     VDELGPKEQLHDLAPGPMGGGPMHMGGGNGVHA"
     gene            5398..6264
                     /locus_tag="M878_RS77105"
                     /old_locus_tag="M878_29595"
     CDS             5398..6264
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="M878_RS77105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_29595"
                     /product="tyrosinase family protein"
                     /protein_id="WP_023550646.1"
                     /transl_table=11
                     /translation="MAYTRKNITRLTATERRRFVGALLDIKRSGEYDEFVRMHIDYNTP
                     DGESGLRTAHMAPSFLPWHRKLLLDLERALRRVDDSVTVPYWDWTRDRSTTALPWTDDL
                     LGGNGRRSDRQVMTGPFAYRAGRWKLTENVTDREYLTRDLGRARDPIELPTAKDLEPAL
                     ADPVYDTAPWDSTVTRGFRNKLEGWGPGQGKVSWRNHNRVHRWIGGVMVGGAAVNDPVF
                     WLHHSFVDLQWTRWQQRHRNHRYLPADPPGERDAQYDRVVARHEQLPPWDVTPDELEDM
                     SGIYRYE"
     gene            6568..7980
                     /locus_tag="M878_RS77110"
                     /old_locus_tag="M878_29600"
     CDS             6568..7980
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (63-328). E-value:
                     7.7e-18. Score: 56.5. Matches aSDomain:
                     nrpspksdomains_M878_RS77110_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1209: polyketide synthase associated protein papA3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019980760.1"
                     /locus_tag="M878_RS77110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_29600"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_031225977.1"
                     /sec_met_domain="Condensation (E-value: 8.5e-14, bitscore:
                     50.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRISDIQRCEARPGHLVEWTFSPATVAAAAALPPDPRPPSYIQES
                     HIRTARSVREDGLFVPTWIGTAFDLPGAADLDALEKALRGWTLRHETLRSGFRWAGDDM
                     HRFTLDEDDVSLHREVVGDFADADTLVRYLQDRFDVAADALGWPNFIYAAVVRDDSTSV
                     YLAFDHTNVDAYSLQRIPDEIHELYRAGVTGRSLAGAPVGSYVDFCEQERANADGIDDT
                     HAIVARWREFIARGDGTLPAFPVDLGLRPGGPLPAQKLMREPLVDADAAAAFEAHCRPY
                     GGSMVGVLAATSLIVHQIGGEPAYRTVVPFHTRVKSAWSDSVGWYVGGAPIEVPAARDF
                     DGALTAVRAELQANRSLARIPLARVLRLLGADFRPTSPDLYSIVSYVDARVVPGSAHWP
                     EQKVYGLLRVSYGDQVCVWITRLHEGLWLACRYPDTDIAYKNLRRYIEALRDLVVSVPA
                     HSRLLATEPTFS"
     misc_feature    6611..6631
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 21.08"
                     /tool="antismash"
     CDS_motif       6757..6861
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_M878_RS77110_0001"
                     /evalue="6.70E-08"
                     /label="C2_LCL_024-062"
                     /locus_tag="M878_RS77110"
                     /protein_end="98"
                     /protein_start="63"
                     /score="25.0"
                     /tool="antismash"
                     /translation="IGTAFDLPGAADLDALEKALRGWTLRHETLRSGFR"
     aSDomain        6757..7551
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_M878_RS77110_Condensation_LCL.1"
                     /domain_subtypes="Condensation_LCL"
                     /evalue="7.70E-18"
                     /label="M878_RS77110_Condensation_LCL.1"
                     /locus_tag="M878_RS77110"
                     /protein_end="328"
                     /protein_start="63"
                     /score="56.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: DHTNVDA"
                     /tool="antismash"
                     /translation="IGTAFDLPGAADLDALEKALRGWTLRHETLRSGFRWAGDDMHRFT
                     LDEDDVSLHREVVGDFADADTLVRYLQDRFDVAADALGWPNFIYAAVVRDDSTSVYLAF
                     DHTNVDAYSLQRIPDEIHELYRAGVTGRSLAGAPVGSYVDFCEQERANADGIDDTHAIV
                     ARWREFIARGDGTLPAFPVDLGLRPGGPLPAQKLMREPLVDADAAAAFEAHCRPYGGSM
                     VGVLAATSLIVHQIGGEPAYRTVVPFHTRVKSAWSDSVGWYVG"
     misc_feature    8047..8060
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.82"
                     /tool="antismash"
     gene            8210..8452
                     /locus_tag="M878_RS77115"
                     /old_locus_tag="M878_29605"
     CDS             8210..8452
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004000202.1"
                     /locus_tag="M878_RS77115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_29605"
                     /product="hypothetical protein"
                     /protein_id="WP_023550648.1"
                     /transl_table=11
                     /translation="MRKLQQVVIAAVAAGGLAGVGAGAGTAYAHGRGVEVNDLYRPYQE
                     CSPQTVADGSLPVSVLGLSQTLDTTCGQFNNAFTG"
     gene            8625..8960
                     /locus_tag="M878_RS77120"
                     /old_locus_tag="M878_29610"
     CDS             8625..8960
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004000203.1"
                     /locus_tag="M878_RS77120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_29610"
                     /product="hypothetical protein"
                     /protein_id="WP_023550649.1"
                     /transl_table=11
                     /translation="MLSRKKLAAVSGLVGGLAVTCTGLAQGYAAADPGVCTRDLQGSVT
                     CIQRIQGEIPENGVIPHQDNCMPMQPITLPAALGNGTIKYGPEVTCSTPPRDKGDGKME
                     FPGGLLD"
     gene            9108..9356
                     /locus_tag="M878_RS77125"
                     /old_locus_tag="M878_29615"
     CDS             9108..9356
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="M878_RS77125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="M878_29615"
                     /product="hypothetical protein"
                     /protein_id="WP_023550650.1"
                     /transl_table=11
                     /translation="MRKFQRAAVVAAAVAGLSVLGVGVSFAGGGEDHEQPSQEYTAVAN
                     SSANAVATYGGYNTHGRGHDDPAPEGGYGAYQGQGGQ"
     gene            9697..10056
                     /locus_tag="M878_RS77130"
                     /old_locus_tag="M878_29620"
     CDS             9697..10056
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004000205.1"
                     /locus_tag="M878_RS77130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_29620"
                     /product="hypothetical protein"
                     /protein_id="WP_023550651.1"
                     /transl_table=11
                     /translation="MFTRQKIATVTGLVGSLAVICVGAAHAHIDGPGRDCRTTAMGDTI
                     CVHKSQTRIDQHGKHVIKQAEDCSVVDRSRLMLPDNDLLGGGSKKVGPVVNCSNEVKLP
                     KGFKKPHFAKPHIGF"
     misc_feature    9703..9717
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.14"
                     /tool="antismash"
ORIGIN
        1 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
       61 nnnnnnnnnn nnnnnnnnnn nnnagcgagg aggcagacgt ctgcgcggcg gacgacacgc
      121 cggatctgtg gtgtcccgcg tgtgcggaac tgggaaagcc gctgcgcgtg gtcgtcgagg
      181 gccggtatcg gctggtgcgc tgccgcgtgt gccgtacaca gtatttccgc gccgaccccg
      241 cgctcgccgg atgcgagggg gaggcaccgt tcaatgagta ctgggagcag tacaagttcg
      301 gcctgtacgc gagtgacgag gtccggcgtg actacgaaga acggtacgag gcgatcctcg
      361 atgaggcgga gcgcctgacc ggacccatcg acacggtgct cgacgtcgga tgcgggatcg
      421 ggaatttcgt cagcttcgcc caggcccgct gcggccgcgc cttcggtatc gacgtggacg
      481 ccgatgccgt ggccgcggcg cgctcccgtg ggctgcgggt cgcgctcagc gaggatctgg
      541 acgcgctgct gcccgacgcc gaggcggacg ccgtcacgct gtgggacgtg atcgagcatc
      601 tctacgatcc gcttcccgcg gtgagccgcg ccctcgggaa actccgtccg ggcggcgcgc
      661 tgatcttcga gacaccggat gcgtcgttcc cggtgcgtcc cgtcctcagg gccgtccata
      721 ccgcgagccg gggccgggtc gatctgacgc ggcacctgta tgactgggag cacaagatct
      781 atttcacggc agccgggatg cgcgccattc tgagccggct ggactgtgag gtgatctcgg
      841 tgcagcgccc ggcgtcaccc cgggcggaga tgcgggagaa cttcacgcgg catgcggacg
      901 cctcgtggca gagccgcgca ctggctcgcg cctggccacg gctggagagt gcggcccgcc
      961 gggtcggccg ggggagcaaa ctcgtcgtca tcgcccggga cgcgggctgc ggatgagagc
     1021 gccggcgtcg gtcctggtcg tggcacggcc gccgcgcggt cagccggacg cccgggcagg
     1081 cagcggattc ggcagcgggt gcggctcgtt cgcgggctcg ggtgcggcgg ggaccggcgg
     1141 attcggatcc tggtccgggc cgcgccgacc ggacagcgcc gcgcggtaga gggcggacgc
     1201 ccgggggttg gccgggcaca gccacacacc gtgcgggcag tagtcggtga tcgtgttgta
     1261 cagcggtttg tgctcgtcga tccacgcgag catccgcagc atgtacgtcg cgttgtcgcc
     1321 gttgcggaac aggccccact cgggatacga gatcggcttc ttgtgcgcct tcgcgaagtc
     1381 gacctggtcc tgcaggccgt agggctcgga cacctgctgg tcgaacgaga tccccgccgg
     1441 ctggtcgtag gagtccatgc cgatgacgtc gacgacgtcg tcgccgggat agcactgcgg
     1501 ccacggaacg gcgtccgcgc cccggtccgg ggcgaagtcg aacgcgaagt gctgcccggg
     1561 caccgagcgc atgacggtga cgatgcgccg ccagtacgtc ttccaggcgt ccggatcggg
     1621 gccgcagcgg tgggtgtagg tgacgccgtt catctcccag ccgagcacga ggaccgtgtc
     1681 cggcaccccg aggcccacga ggtgctcggc gagcgtccgg aagtgctggt cgaactcgcc
     1741 ggccgcgccg cgccgcagca cctgctggac ctccgtgtcc ggcacatggc cctcgtcgcg
     1801 ttccgtcaac gggacgttga ccacgaggat gcggtcgtcc ttggcccgcc gccactgggc
     1861 ccagttgccg aggaagcccg gcgtgccctc gatgttgctc cacttgtccc ccggcaggta
     1921 cgtgtgcccc acgcgcacct cggtgtggtc gagccagtcg ctgaatcccc ggatgcgcag
     1981 cacaccgagg ggcccggaac tgaggtacgc gccgaacgcg ggcgggcgcg gctcccccga
     2041 ggagggaccc gtcgccccgt tcgccgcgca ccctgcgggg acgggagcgg cgaacccggc
     2101 cgcgcagtgc tgcgcggccg ggttcgccgt ggggccggac ttgatgacag ggctgggaag
     2161 gacgctcgcc gccgcgaaca ccccggcggc gaggaatgtc actcgcctca gggatcgcag
     2221 acgctcggac gccattccct actccttttg gtgcgactac accccaatta ctgggacaca
     2281 gccatatata cgctcattgc accaatggag tcggatggct cgacgccgct agtccacaag
     2341 gacgaccgta cgagatggtt ttccgtcggc gggtgccggg cgcggggcgt ccggcacccg
     2401 ggcgactact gctgggctgt ggtcttgcgg cctcgccggt agaggacggt tccggcgacc
     2461 agcagcgcgc cgctggcggc gacggctgcg atggtgccct cggtgccggt gtgggccatc
     2521 ggcctgtggt ggtggtgatg gtggtggtgg tgccggtggt gcggcttctc gggcctctcc
     2581 ggcggcatgt ggtggtgatg gtggtggtgc accggcttgg gctcgtggtg gtgatgcggg
     2641 ggcttttctt cgttgccgta gctcgactcg tcgtcaccgt agttcgactc gtcgtcgccg
     2701 tagttcgact tgtcgtcgcc gtagctttcg tgggacggct tgtggctcgg cttctcgtcg
     2761 tcgccgtagc tttcgtggga gggcttgtgg ctcggcttct cgtcgtcgcc gtagctttcg
     2821 tgggaggagt gctcgctcga ctcgtcgtcg ccgtagtcct cggaggagct ctcgcgatgc
     2881 gacttcgtca catggccggt ggaccagccg ggggaatacg aggacgagaa ctccgacgcc
     2941 aagttctgcg ccatctccgt ggcgctctcc gcgcgctcca tcgcccgcat ggcgcttgcc
     3001 gcacccggga aatcggagtc gccgacgtcg tccgaatagg aggaatacgc gtcgtcggac
     3061 gtgaacggac cggagtccgc gaacgcccag gggctgtata cagtcaggac gctcgtcgcg
     3121 gcggcggcca cgaccaatcc cttggtcagg gtgtgtcgca ctctctttgt cttcctctca
     3181 gggggagtcg aaaggccggc cctggagcca gtgaaggggg actgctccgc agtgggccgc
     3241 ggggttccgt aggttcgtcg cagggccgtg gtgccgcacg gatgccgcac gttcacacgt
     3301 gccaccactt gcagactgtg aacgaaatat gtacttccgc ggatacctgg aagcatgaaa
     3361 tgccgggtaa ttcactcgat cgcccgtctt ggcttgacgg gctcacccgc cgtacagggc
     3421 ctcgatctgc tccgcttacg cggtcgtcac ggcctgtcgt ttcaccttca acgacgaggt
     3481 gagcggcctg ttctcctcac tgaactcgcc ctcgaccagt gcgaaggcgc ggatcgactc
     3541 ggcgtgtgag acagccgtcg ctgtccaggg agccgaggtc accggtggcg agccggccgc
     3601 cggtgagcac cgcgtcggcg gccgcctcgg cgtacgagtg tgggaagcgc ctcgccgtcg
     3661 ggccgccagg tgcgcggagg ttcggcgtgg ccggccgtgc cggcggcgac cgtctcggcg
     3721 atgacgaaac gggcgccgga gtcgcgcacg atccactcca cctgctcggc cgaagaggtg
     3781 gcgcagacgg ggacgctctg gccaccggcg gccagagcgc gaagtccagg accgtccact
     3841 cgtaacgggt gcgagacatc acggcgacgc gccgcccgat ttcatcggcg ggtggttgac
     3901 atccctgcgt acgcctatga cggcccctca gtcagcttgg tcgggagcca acttgctcac
     3961 gcgcaacaga agctcaacgg cgccgcaagc agcggcggcc acgttcgagg gacgtcgcgg
     4021 cgcgtcccct gatcgtgtgg agccccggcc gccctcctcc ggtcgggtga gcgcacaccg
     4081 ggccgccccg tcgccgatcg gtagggtcgc gcggtgacct caacccccgg tgacacgcct
     4141 ccgtcccgcc ccgcagaccc gggcaccccc gcccggcccg cgccggcggc gggcgccggc
     4201 cgcggcttcg cgctgctcct ggtgctcacg ggcgcggccg gactgctggc ctcctgggtc
     4261 atcaccatcg acaagctcaa gctgctccag aacccgaact tcacgccggg ctgcagcctc
     4321 aatccggtgg tgtcctgcgg cagcgtcatg aagagcccgc aggcctcggt cttcggcttt
     4381 cccaacccga tgctcggcct ggtcgcctac ggcatcgtga tctgcgtcgg catcagcctg
     4441 ctggccggcg ccgccttccc gcgctggtac tggctgatct tcgacgcggg ctgtctgttc
     4501 ggggtcgggt tcgtgtcctg gctgcagttc gagtcgctgt accggatcaa cgcgctgtgc
     4561 ctgtggtgcg ctctggcctg ggtcgccacg atcctgatgt tctggtacgt cacctcgttc
     4621 gccgtacggc accggttcct gcccgcgccg ggcccgctgc gggcgttctt cgccgagttc
     4681 acctgggtac tgccgctgct gcacatcgcg gtcgtcggca tgctgatcct gacccgctgg
     4741 tgggacttct ggaccagctg agtcgtccgg ctccggcggc cggtccgccg cggccccggc
     4801 gggcgcgtca ccggtccggc ggtcaccggt ccggccgcac ggcggtgacc gggagggcca
     4861 cccggcggtt accgcctcgg acaaccgaac cggacgagga gtgaacgatg gttccaggtg
     4921 tcggcggagc gccggtgggc gggcagagcg gcacgccggc cgcggacgga gcgcggcccc
     4981 ggggcagcac acggcgggac gtgacgcgga gactgttctc ctcggcgctc gtcgtggcgc
     5041 tcgccccggt gaccgcggcc tgcgcacggc gagccgagca tgccccggac gacggccgac
     5101 gggccaccgc cttcgacgag acctaccgag gccgccacat ccgcggcgtc gaggtgcacc
     5161 ccgccgagcg ggccgccgtc gccggcgccc agtggaaggt cacgatcgac ggccacccgc
     5221 tgcatctgat gcgccgggcc gacggcagct ggctgagcat gatcgaccac taccgctcgt
     5281 acccgaccgc gctcgccgcg gcccgcgggg cggtcgacga actgggcccc aaggagcagc
     5341 tgcacgatct ggcaccgggg ccgatgggcg gcgggccgat gcacatgggg ggcggcaatg
     5401 gcgtacacgc gtaagaacat cacccggctg accgccaccg agcggcgccg gttcgtgggc
     5461 gctctgctgg acatcaaacg cagcggtgag tacgacgagt tcgtgcggat gcacatcgac
     5521 tacaacaccc ccgacggtga aagcgggctg cgcaccgctc acatggcgcc ctccttcctg
     5581 ccctggcacc gcaagctcct gctggacctg gagcgggcgc tgcgccgagt ggacgattcg
     5641 gtgacggtgc cgtactggga ctggacccgc gaccgctcga ccaccgcgct gccgtggacc
     5701 gatgacctgc tcggcggcaa cgggcggcgc tcggaccggc aggtgatgac cggcccgttc
     5761 gcctaccggg ccggccgctg gaagctcacc gagaacgtga ccgaccggga gtacctcacc
     5821 cgcgacctcg gccgagcccg cgacccgatc gagctgccga ccgccaagga cctggagccg
     5881 gcgctcgccg atccggtcta cgacacggcg ccctgggact cgacggtcac caggggattc
     5941 cgcaacaagc tggagggatg ggggccgggc cagggcaagg tctcctggcg caaccacaac
     6001 cgggtgcacc gctggatcgg cggggtgatg gtcggcggcg ccgccgtcaa cgaccccgtc
     6061 ttctggctgc accactcctt cgtcgatctg cagtggaccc gctggcagca gcgccaccgc
     6121 aaccaccgct acctcccggc cgacccgccg ggcgagcgcg acgcccagta cgaccgggtc
     6181 gtcgcccggc acgagcaact gccgccgtgg gacgtgacac cggacgagct ggaggacatg
     6241 agcgggatct accggtacga gtgaggccgg tccgcgggag ccgccacgcg gcaagagccc
     6301 gaagagcccc cgcccgcacg acgccggggg cttttcggtc acgtacgacg attccgtcaa
     6361 ctcagccttt ttctgcgcct gttcagtcgg catgccggtc gacagcggtc gtaccgcacc
     6421 gcgttgccgg cctcgatcac ctcgaacgtt cgcttcgccg ttcgaggcat cacacgtccc
     6481 gctgaaatgc cggcgacacg gcaaccgccg cccggtgcgt ggctctccgc tgatcaagtc
     6541 tgggtcttct gcgaaggaga cgcccctgtg cgaatttctg acattcagcg ctgcgaagcg
     6601 cggcccggac atctcgtcga gtggacgttc agcccggcga ccgtcgcggc cgcggcagcg
     6661 ctgccgccgg atcctcggcc gccgtcgtac atccaggagt cgcacatcag aaccgcgcgt
     6721 tcggtgcgcg aggacggact cttcgtgccg acctggatcg gcacggcgtt cgacctgccg
     6781 ggcgcagccg atctcgatgc gctggaaaag gcgttgcgcg gctggaccct gcggcacgag
     6841 acgctgcgca gcggcttcag atgggccggc gacgacatgc accggttcac gctcgacgag
     6901 gacgacgtgt ccctgcaccg cgaggtggtc ggcgacttcg ccgacgcgga cacgctggtc
     6961 cgctatctgc aggaccgatt cgacgtcgcg gcggacgcgc tcggctggcc caacttcatc
     7021 tacgcggcgg tggtccgcga cgattccacc agcgtgtacc tggcgttcga ccacaccaac
     7081 gtcgacgcct actccctgca gcgcatcccg gacgagatcc acgagctgta ccgggcgggc
     7141 gtcacgggcc ggtccctggc cggggcgccc gtgggcagtt acgtcgactt ctgcgagcag
     7201 gagcgggcga acgccgacgg catcgacgac acccacgcga tcgtcgcccg ctggcgggag
     7261 ttcatcgccc ggggcgacgg cacgcttccg gccttccccg tcgacctcgg gctgcgccca
     7321 ggcggtccgc tgcccgccca gaagctgatg cgcgagccgc tcgtcgacgc ggacgccgcc
     7381 gccgcgttcg aggcgcactg ccggccctac ggcggcagca tggtgggcgt cctggccgcg
     7441 accagcctga tcgtgcacca gatcggcggg gagccggcct atcgcacggt cgtaccgttc
     7501 cacacccggg tgaagtccgc gtggtcggac tccgtgggct ggtacgtcgg cggcgcaccg
     7561 atcgaggtgc ccgcggcccg ggacttcgac ggcgcgctca ccgccgtccg cgcggagttg
     7621 caggccaacc ggtcgctggc gcgcatcccg ctggcccgtg tactgcgcct gctcggcgcg
     7681 gacttccgtc cgacctcgcc cgacctgtac tcgatcgtct cctacgtgga cgcccgcgtc
     7741 gtccccggct cggcccactg gccggagcag aaggtgtacg ggctgctgcg ggtgtcgtac
     7801 ggcgaccagg tgtgcgtctg gatcacccgg ctgcacgagg ggctgtggct ggcctgccgc
     7861 taccccgaca ccgacatcgc gtacaagaac ctgcggcgct acatcgaggc gctgcgggat
     7921 ctcgtggtgt ccgtgcccgc gcacagtcgt ctcctggcca ccgaaccgac attttcgtaa
     7981 taccgggttg gtcaacttcc cttttcacca tccgcgttga cgtgcctttc agtaacccgc
     8041 aatgcgggca cggcagttgc ctctgccggg cacggctttc gtacccatgt ccctttttgg
     8101 acacggatat ccggcaaagt ggaataccgt gtatcgctac tggtagtgtt cactcagtca
     8161 cggtaggcaa tcagcctcga tgtccaagca aggaagaacc gagggttcca tgcgcaagct
     8221 tcagcaagtc gtgatcgcag cagtggcggc cggcggtctg gccggcgtcg gtgccggcgc
     8281 cggtacggcc tacgcccacg gtcgcggggt tgaggtgaac gacctctacc gcccctacca
     8341 ggagtgcagc ccgcagacgg tggccgacgg cagcctcccg gtctcggtgc tcggcctctc
     8401 tcagaccttg gacaccacct gcggtcagtt caacaacgcc ttcactggct gaggtcgacg
     8461 gcgtcggcac gcgcgacgcc gtccgcggaa cccttcgggt gaccctccga agggttcccc
     8521 tcacgcccgg ccggtcgccc ggctcccagg acgaagtcag ggcagggcgc ggctggttcg
     8581 cggcgcacgg tgccctggcg cccctcacaa aggaaatgca acacatgctc agtagaaaga
     8641 agctcgcggc cgtctcgggg ctcgtcggcg gcctcgccgt gacctgcacc ggcctcgccc
     8701 aggggtacgc cgcggcggac ccgggggtct gtacgcgcga ccttcagggc agcgtcacct
     8761 gcatccagcg gatccagggc gagatccctg agaacggcgt catcccccac caggacaact
     8821 gcatgccaat gcagcccatc accctgccgg cggccctggg caacgggacc atcaagtacg
     8881 ggcccgaagt tacctgctcc acgccgcccc gcgacaaggg cgacggcaaa atggaattcc
     8941 ccggcggcct gctggactga gactccgggc cggaagctcc cgacgaaagc cttgcgcaga
     9001 cccgacacga cgtatggccg aagatcccaa attgttactg atcgtgtgta tatgctcacg
     9061 ataagtagtc aaccagggct gtgtgcccgg gcaagaaagg gtcaatcatg cgtaagtttc
     9121 agcgtgcagc ggtcgtagcg gcggcggttg cggggctgtc cgtcctcggc gtcggtgtca
     9181 gcttcgccgg cggcggtgag gaccacgagc agccgtcgca ggagtacacc gcggtggcca
     9241 actcgtcggc caacgccgtg gccacgtacg gcggctacaa cacccacggc cggggccacg
     9301 acgaccccgc acccgaaggg ggttacggcg cgtaccaggg tcagggcggc cagtaagacc
     9361 aacccggtcc acgatgcgca cgagtgagcg cgcggagcgg ccgtagcgca tcggcattcc
     9421 tttggaacgc gatatgcaca tcgtacgaac tgtatctccc agtcccggac caactcggat
     9481 tgttcgccgg tgtgctgagt aataccccat ggcgaaggca gcccggacgc gtgacagacc
     9541 cctcagggtg cgcgtccggg ctgcggcaat gccgccgccg gacgcattcg ttcttgcgtc
     9601 caggcgctgg gtcccccttc cgaccttctc caaggcccgg gtgtgcgccg gtggatggcg
     9661 cagacgccgg gccttcagcg caaaggaaca cagcacatgt tcactcggca gaaaattgcg
     9721 accgtcaccg ggctcgtcgg aagtctcgcc gtgatctgtg tcggcgccgc acacgcacac
     9781 atcgacggcc ccgggcgtga ctgcaggacc accgccatgg gcgacaccat ctgcgttcac
     9841 aagagccaga cccgaattga ccagcacggc aagcacgtca tcaagcaggc ggaggactgc
     9901 tcggtcgtcg accggtcgcg cctgatgctg cccgacaacg acctgctggg cggcggatcc
     9961 aagaaggtcg ggccggtggt gaactgctcc aacgaggtaa agctgcccaa gggcttcaag
    10021 aagccgcact tcgcgaagcc ccacatcggg ttctgacgcg cgatcgtagg cgtctgacgc
    10081 gcgatcggaa aatgccggac cgggatcgtc ccggtccggc atttcggctt tctggggcag
    10141 gatatgcccg ctacatggaa ttccccggtg cctcggcctc gcggccggga caccggggaa
    10201 agggttccgc ttacctgctt actcgcttac ctgctcactt gccgacgctg ttgttgcagc
    10261 ccatgttcga gccgagctgc gactcctgcg caccagcgtt gccctcgccg ttcagcaggt
    10321 tgccgcccag gccgttgagg atgccgacct cgccgaggac gtcggcgttc agatcgtggg
    10381 acctgcaggt cgtgctctgc aggacgctga agcg
//
