LOCUS       NZ_CM002285            11029 bp    DNA     linear   CON 29-APR-2024
DEFINITION  Streptomyces roseochromogenus subsp. oscitans DS 12.976 chromosome,
            whole genome shotgun sequence.
ACCESSION   NZ_CM002285
VERSION     NZ_CM002285.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseochromogenus subsp. oscitans DS 12.976
  ORGANISM  Streptomyces roseochromogenus subsp. oscitans DS 12.976
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9663240)
  AUTHORS   Ruckert,C., Kalinowski,J., Heide,L. and Apel,A.K.
  TITLE     Draft Genome Sequence of Streptomyces roseochromogenes subsp.
            oscitans DS 12.976, Producer of the Aminocoumarin Antibiotic
            Clorobiocin
  JOURNAL   Genome Announc 2 (1), e01147-13 (2014)
   PUBMED   24407645
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663240)
  AUTHORS   Ruckert,C., Szczepanowski,R., Heide,L., Kalinowski,J. and Apel,A.K.
  TITLE     Draft genome sequence of Streptomyces roseochromogenes subsp.
            oscitans DS 12.976
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 9663240)
  AUTHORS   Ruckert,C., Szczepanowski,R., Heide,L., Kalinowski,J. and Apel,A.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2013) CeBiTec, Bielefeld University,
            Universitaetsstr. 27, Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.5.3
            Assembly Name                     :: S.roseochromogenes_v1
            Genome Coverage                   :: 20.4x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000497445.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 03:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,098
            CDSs (total)                      :: 9,013
            Genes (coding)                    :: 8,393
            CDSs (with protein)               :: 8,393
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 6 (16S, 23S)
            complete rRNAs                    :: 6, 6 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 620
            CDSs (without protein)            :: 620
            Pseudo Genes (ambiguous residues) :: 0 of 620
            Pseudo Genes (frameshifted)       :: 227 of 620
            Pseudo Genes (incomplete)         :: 534 of 620
            Pseudo Genes (internal stop)      :: 54 of 620
            Pseudo Genes (multiple problems)  :: 178 of 620
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:03:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5656728
            Orig. end                         :: 5667757
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CM002285.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11029
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="30"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11029
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11029
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6029](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6029
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            509..1741
                     /locus_tag="M878_RS74155"
                     /old_locus_tag="M878_26310"
     CDS             509..1741
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /locus_tag="M878_RS74155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_26310"
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_023549821.1"
                     /sec_met_domain="ATP-grasp_3 (E-value: 2.1e-09, bitscore:
                     36.7, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTLIIGGAAGAATGYVHDICARALAQLRARGGRIVLTDLPENL
                     AAAPELTQLADDIVELDFTDAEACVAWATAHAERHRVDAVLAFREYAVVAAAEVAAALG
                     LPGNPPEAVRTVRLKDVCRERLRELGFRQPQLRLCRSAEEAADFHREVGGTVIIKPRSG
                     SSSEGVQQVEDPRRMAAAFAASCDAEGVALVETFVGGPEFSVEGLITGGRPRVLAVTAK
                     QVLPVSFVECGHTMPAPIGPEATEAITAEVVGALAALGLRYGPFHVECWLTGEGVVLGE
                     LHVRQGGDWIHAMTEWVNPGLELYGSWYDDLLGRPVTLPEAPTRGAAARFVTLPSGTVS
                     SVTGWSGIASHPDVRTADLAVAPGATLHPTTSNLERHGVFVVGTPDPSDAGPLADKLLA
                     GLEVRVVPTGG"
     CDS             complement(1898..>2168)
                     /codon_start=3
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="M878_RS97695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_158692772.1"
                     /transl_table=11
                     /translation="LAPERTEALIERFLDAHLMLDGGTGAGFRIPSLVRLACLGPTASA
                     PAASCPQSAAPPRPLPDRRLSPRSRRTVPAGSRAHCRTASYSVRD"
     gene            complement(1898..>2172)
                     /locus_tag="M878_RS97695"
     assembly_gap    2173..3070
                     /estimated_length=898
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     gene            complement(<3071..4771)
                     /locus_tag="M878_RS74160"
                     /old_locus_tag="M878_26320"
     CDS             complement(<3071..4771)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /locus_tag="M878_RS74160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_26320"
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_023549822.1"
                     /transl_table=11
                     /translation="MEFSVLGPVEVRDGIEDLTPAAAMPRRVLATLLLNAGRVVSHATL
                     IEELWDEEPPRLARKTIQTYLYQLRKALASGPTGSSGPSGSWGSSGSWGSSGSSGSSGS
                     SGSSGEGRQWLETRPNGYRLLLQPGELDLHTFQQRVALGRSALADGDHARAAQLLREAL
                     GLWRGDALDDVPHGPVLAAEVARLGETRLATLQQRIEADLRLGLHRELVGELQTLTRRH
                     PLREEFSAQLLLAAYRSGLREEALSCYARLRRAVVEHTGLEPSERLQRLHSAVLADAPG
                     LSAPERPAAPRPGSRPIRPAEIPAGIAEIVGRAAETEALERQLARSAALVPLVGAPGTG
                     KTALAVHVAHRVRERFPDGQLFLTLHDAEGRPLEPLAALRSMLRTIAPGEPPDSLEEAA
                     RMFRSWTADRRVLVFLDDAASVQQVLQLLPSGQKCATIVTSRRRLGGLPGGGVRLGRLT
                     AKDALALLGSVAGRERVERERGAATELVTWCEGLPLAVWTAGQRLAARPALGVGELAAR
                     MRAEQQRLGELKAGDVDVRQRIGAAVRRLGGADERALRALAALGDRGLDL"
     gene            5001..6029
                     /locus_tag="M878_RS000000100140"
                     /old_locus_tag="M878_26325"
     CDS             5001..6029
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="M878_RS000000100140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_26325"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_023549823.1"
                     /sec_met_domain="AfsA (E-value: 2e-21, bitscore: 74.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRHSSNTLVDPAAAASSGLFEQPVPRSLVHRASVSEVFLTSIHPD
                     GPDRFLISAQWPRQHSFYRSRHGFHDPLIFAETIRQAGLIIGHAGYQVPLANPFILNGM
                     EFDITPEGLARGGAPAHLMLEARCENIQRRGERLLGLDYRIDLYRDMVRVGTARCGFTC
                     VSPAVYRRLRGSAAGAGTGAGTGAGAGAGVDVGVDVGAGAGAGAAPLRDELPPPVAPEL
                     VGQESAGDVVLSAMDRPGSWLLRCDPDHPVLFDHPVDHVPGMVVLEAMRQAAFARSGSR
                     PLLVGCAMEFLRYAELGLPTEIHAAPEPARADGTVPVRVAAAQNGDIVAQGVLRVRPDS
                     GA"
     gene            6035..6169
                     /locus_tag="M878_RS000000101100"
                     /old_locus_tag="M878_26330"
     CDS             6035..6169
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="M878_RS000000101100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="M878_26330"
                     /product="hypothetical protein"
                     /protein_id="WP_023549824.1"
                     /transl_table=11
                     /translation="MRPILTALLRRSGLAVRAWLRGSRPVMLRVPFAYPVRSSAEDDV"
     gene            complement(6338..7108)
                     /locus_tag="M878_RS74170"
                     /old_locus_tag="M878_26335"
     CDS             complement(6338..7108)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891815.1"
                     /locus_tag="M878_RS74170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_26335"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_023549825.1"
                     /transl_table=11
                     /translation="MPGGRLTQQERQQIALGLADGLAYAEIARRLGRPTSTITREVMRN
                     GGPTAYRADLAHRATERRAHRRRQTAPRDPKALPRTHGRDAEAVREYEEAFTTLLMQQG
                     LPKMMSRVLTCLYTTDAGSLTASELVQRLQVSPASVSKAVTFLAGQGLIRRERDERRRE
                     RYVVDTDVFYQGMMAAARSNAQLAETARQGVGLLGPGTPAATRLENIARFVDFVAESIA
                     RAAEQARDILYTEPAYTKSETASDGTATPSSDRG"
     gene            7354..8022
                     /locus_tag="M878_RS74175"
                     /old_locus_tag="M878_26340"
     CDS             7354..8022
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029635.1"
                     /locus_tag="M878_RS74175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_26340"
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="WP_031225668.1"
                     /transl_table=11
                     /translation="MRKFDTPAPIAAVLDIPAGRVQLIAADRGDTTVEVLPADASKGRD
                     VKAAEQIEVVYGDGVLRVEAPAAKNRLLGHSGFIEVTIQLPAGSRVEAKAASAEFRGVG
                     RLGDVTFEGAQGSVKLDETEGARLTLQAGDVSVGRLGGPAEISTQKGDIHIAEAVRGTV
                     TLRTQAGDVSVGAAAGVSASLDAGTSSGRIHNALKNADGAPGLNIHVTTACGDIAARSL
                     "
     gene            complement(8160..8804)
                     /locus_tag="M878_RS74180"
                     /old_locus_tag="M878_26345"
     CDS             complement(8160..8804)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039544.1"
                     /locus_tag="M878_RS74180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_26345"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_023549827.1"
                     /transl_table=11
                     /translation="MTSKITIPGWTGSPTEDRLPDLRQERAVQTRAQVLRAAAEEFAEK
                     GFKSTSIQDVAKRVGMTKGAVYFHFPNKEILAIAVVEAHYSRWPELLEGILAEGHPPLD
                     TVVRVLDGAAKAFATDIVVQAGARLQLERSLIDAQLPQPYVGWTQLLAHLLTSAKEAGE
                     LRAGVDPEAAARALVSGFFGVQHVSDTLTGRADLLDRWPEFRDLVMTSLRA"
     gene            8919..>9735
                     /locus_tag="M878_RS97705"
                     /old_locus_tag="M878_26350"
                     /pseudo=""
     CDS             8919..>9735
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1191: acyl-CoA dehydrogenase type 2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="M878_RS97705"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="M878_26350"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRSLCEPCVLASGRAGGGERMGIGTLMDGAQLAEGGLRTARVAQL
                     ARLARASAARVDAERRLSPELAAEITAAGFPAHFVPRRWGGAAAGFRQLVDAVAALGEE
                     CVATAWCAALYAAHGRLAAYLPERGQRELWDTSPDVRIAAAVVPPAGRAVPEGEGWRIS
                     GEWHYASGVDHADWVLVAVRVEGADGGGSEVRCFAVPRRELVIKDSWHSLGLRGTGSNT
                     VVAGGAGVLVPRHRSFTLADLDTVRAGWPAATPCPSGSSPRSSSPRRSWG"
     assembly_gap    9736..9760
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     gene            <9761..10184
                     /locus_tag="M878_RS94750"
                     /pseudo=""
     CDS             <9763..10184
                     /codon_start=2
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /locus_tag="M878_RS94750"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="oxidoreductase"
                     /pseudo=""
                     /transl_table=11
                     /translation="PRGAVAPEVLARADGEIEAARLLLGAAADRADAAGLGPDAVARNQ
                     RDAALAAELCTTAVDRLFRAGGTRVHFDGDPLQRAWRDLTTGASHATLAFGTAAAAFMA
                     ERTAAFEAGEAAASGWGADGGTAIPAGTSFPTGDSR"
     gene            10181..10954
                     /locus_tag="M878_RS74190"
                     /old_locus_tag="M878_26360"
     CDS             10181..10954
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /locus_tag="M878_RS74190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_26360"
                     /product="HAD-IB family hydrolase"
                     /protein_id="WP_023549829.1"
                     /transl_table=11
                     /translation="MTNAHPHRAPHQHSDPGSTARPTCCPLLRPHPQSHPARIAFFDVD
                     HTLTTVTTPALFWEYWSTAARTAPLAAGRALPAAAGVAREERNRDYYRLFAGVPAMELG
                     RAGARWYGGFRVTAQPFLPAPLAALRAHRAAGDLVVLVSGAPRACLLPLARDLGAGLVL
                     GCEQLTDRWGRLTGEITRPMIGAAKADAAAEVMARHGADPADCHAYGDHSSDLPLLRLC
                     GHPTVVGDDPVLAGHTERDGWERLPGVESLVRIRK"
     misc_feature    complement(10965..10978)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.16"
                     /tool="antismash"
ORIGIN
        1 gtcgcgcagc gccccgacca cctccggggc caggccgggc aggccgaggt cccgggccag
       61 atgggcgcag gtgtccacgg cgtactcgta gcccggcacc acggcgacca ggtcggtgcg
      121 ggcggccagc tcccggctcg cccggagcac ggtcggcagg tcgcggatct cgacgcgggt
      181 gtagtcggcc cagccggagt gcacggccgc gcgcacctgt gcgggcagct cggccacggg
      241 cttgcggtcg aacacgtgcg gccggaggcc gaggcggcgg gcggcgtcga ccaggccgat
      301 cccggaggag ttcggctcga cgatcagggc tgtctggtgg gtgctgcggg cggacatggc
      361 gctcctcggg cagtcagggc cggcagggca gtcacgacga aagggaaggc aagacatctc
      421 gagtagctct gatgtcttgg cgatgtcatc ttaagcatct aagatgacag atgtgaaggg
      481 ccgtcgtaca ccgaggtgat gaggaaccat gaccacgaca ctgatcattg gcggggccgc
      541 cggagcggcg accgggtacg tgcacgacat ctgcgcgcga gccctggccc aactccgcgc
      601 ccggggcggc cggatcgtgc tgacggacct cccggagaac ctggccgccg cccccgagct
      661 gacccaactg gccgacgaca tcgtcgagtt ggacttcacc gacgccgagg cctgtgtggc
      721 ctgggcgacc gcccacgccg aacggcaccg cgtcgacgcg gtgctggcct tccgtgagta
      781 cgcggtcgtc gccgccgcgg aggtcgcggc cgcgctcggc ctcccgggca acccgcccga
      841 ggcggtacgg acggtgcgcc tgaaggatgt ctgccgcgaa cgcctgcgcg aactgggctt
      901 ccgtcagccg cagttgcggc tgtgccggag cgctgaggag gcggcggact tccaccgcga
      961 ggtgggcggc accgtgatca tcaagccgcg cagcggttcg agcagcgagg gcgtgcagca
     1021 ggtcgaggac ccgcggcgga tggcggcggc gttcgcggct tcctgcgacg cggagggcgt
     1081 cgcgctggtg gagaccttcg tcggcggccc cgagttcagc gtcgaggggc tgatcaccgg
     1141 cgggcggccg cgggtgctcg cggtgaccgc gaagcaggtg ctgccggtgt ccttcgtgga
     1201 gtgcggccat acgatgccgg cgcccatcgg ccccgaggcg acggaggcga tcaccgcgga
     1261 ggtcgtcggc gcgctggccg cgctgggtct gcggtacggc ccgttccacg tcgagtgctg
     1321 gctgaccgga gagggcgtgg tcctcggcga gctgcatgtg cgccagggcg gcgactggat
     1381 ccacgcgatg acggagtggg tcaatcccgg tctggagctc tacggctcct ggtacgacga
     1441 cctgctcggc cgccccgtca ccctgcccga ggcgcccacg cgcggtgcgg cggcacgctt
     1501 cgtcacgctc ccctccggca cggtctcatc cgtcaccggc tggtccggca tcgcctccca
     1561 ccccgacgtc cgcaccgccg acctcgccgt cgcccccggc gccacgctcc accccaccac
     1621 ctccaacctc gaacgccacg gcgtcttcgt cgtcggcacg cccgacccgt cggacgcggg
     1681 cccgctggcc gacaagctcc tggcggggct ggaggtacgg gtggtgccga cgggcggatg
     1741 agcagccggc cgtcccgtac ccggccggtc ccgtacctgg tcccgtaccg ggtcggtccc
     1801 gtacctggtc ccgtaccagg ttggtcccgc acctggtgcg acgccgtacc cctacccctc
     1861 ggtcccgcac ctggtacgac gccgtacggc aatgcgctca atcccgtacc gagtacgacg
     1921 ccgtacggca atgcgcacgg gaccccgcgg gaacggtccg gcgggagcga ggggagagtc
     1981 ggcggtcggg gagagggcgg ggcggggcgg cggactgggg gcaggacgcg gcgggcgcgg
     2041 acgcggtcgg gccgagacag gcgagcctga ccagggacgg gatgcgaaag cccgccccgg
     2101 tgccgccgtc cagcatcaga tgggcgtcca ggaaccgttc gatcagcgcc tccgtccgct
     2161 cgggcgcgag gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn gaggtccagc ccgcggtcgc cgagcgcggc cagcgcccgc agcgcccgct
     3121 cgtcggcccc gccgagccgt cgtaccgcgg ctccgatccg ctgccgtacg tccacgtcgc
     3181 ccgccttcag ctcgcccagc cgctgctgct ccgcccgcat ccgcgcggcc agttcgccca
     3241 ccccgagcgc cgggcgcgcg gccagccgct ggcccgcggt ccacaccgcc agcggcaacc
     3301 cctcgcacca tgtgaccagt tcggtcgccg caccccgctc ccgttccacc cgctcgcgcc
     3361 ccgcgaccga gccgagcaag gccagtgcgt ccttcgcggt gagccggccg agccggacgc
     3421 cgccgccggg cagcccgccg agccgccgcc ggctggtcac gatcgtggcg cacttctgcc
     3481 cgctgggcag cagttggagc acctgctgga cggaggcggc gtcgtcgagg aagaccagca
     3541 cccgtcggtc ggccgtccag ctgcggaaca tccgcgcggc ctcctccagc gagtccggcg
     3601 gctcgccggg cgcgatcgtg cgcagcatgc tgcgcagcgc cgccagcggc tcaagcggcc
     3661 ggccctcggc gtcgtgcagg gtgaggaaga gctgcccgtc ggggaaacgc tcgcgcaccc
     3721 ggtgcgccac gtgcaccgcg agcgccgtct tgccggtgcc gggggcgccg accagcggga
     3781 cgagcgcggc ggagcgggcc agttggcgtt ccagcgcctc cgtctcggct gcccgtccga
     3841 cgatctcggc gatgccggcg ggaatctccg ccggccggat cggccgggag cccggccggg
     3901 gcgcggcggg ccgctccggc gcgctcagac cgggggcgtc ggcgagtacg gcgctgtgca
     3961 gccgctgcaa ccgctcggag ggttcgagcc cggtgtgctc tacgacggcc cggcgcagcc
     4021 gcgcgtagca gctgagcgcc tcctcccgca gcccggagcg gtacgcggcg agcagcagct
     4081 gcgccgagaa ctcctcccgc agcgggtgcc ggcgcgtcaa cgtctgcagt tcgcccacga
     4141 gttcgcggtg cagaccgagg cgcaggtccg cctcgatccg ctgctgcagg gtggccagcc
     4201 gggtttcccc cagccgggcc acttcggcgg cgaggacggg gccgtgcggt acgtcgtcga
     4261 gtgcgtcgcc gcgccacagg ccgagtgcct cgcgcagcag ctgggcggcc ctcgcgtggt
     4321 cgccgtccgc gagtgcggag cggccgagcg cgacgcgctg ctggaaggtg tgcagatcga
     4381 gctcgccggg ctggagcagg agccggtagc cgttcggacg ggtctccagc cactgccgtc
     4441 cctcgcccga ggaacccgat gaccccgagg aacccgatga ccccgatgag ccccatgagc
     4501 ccgaggaacc ccatgagccc gaggggcccg atgagcccgt ggggccggag gccagggcct
     4561 tgcgcagttg gtagaggtag gtctggatgg tcttccgggc cagccgcggc ggctcctcgt
     4621 cccagagttc ctcgatgagc gtggcgtgcg agacgacccg gccggcgttg agcagcaggg
     4681 tggcgagcac acggcgcggc atggccgccg cgggggtcag gtcctcgatt ccgtcacgca
     4741 cctcgacggg tcccagcacg gagaactcca cgggccgtcc ctccagtctc cgtaagatac
     4801 cttcgagaag gttttataac tgctcaccgt tccgcggtca acaacagtcc acaaccggag
     4861 gtgccgatcc ccttcaggct gcggcttgtt gggcgatctt gggatgtgag acaccgcttg
     4921 aacggtgaat attctgtccc gtaggttttt ttcgcgagcc aagttgccca ctgaggtgcc
     4981 actaggaggc tgactctgtc atgcgccact caagcaacac gctggtcgac cctgccgccg
     5041 ctgcttcctc ggggctcttc gagcagcccg ttccgcgcag cctggtgcac cgggcctcgg
     5101 tctccgaggt cttcctcacg agcatccatc ccgacggccc cgaccgcttc ctgatcagcg
     5161 cgcagtggcc ccggcagcac agcttctacc gcagccggca cggcttccac gacccattga
     5221 tattcgccga aacgattcgg caggccggcc tgatcatcgg ccacgccggc taccaggtcc
     5281 cgctcgccaa ccccttcatc ctcaacggga tggagttcga catcacgccg gagggcctcg
     5341 cccgcggcgg cgcccccgcg cacctgatgc tggaggcccg ctgtgagaac atccagcgcc
     5401 gcggcgagcg gctgctgggc ctggactacc ggatcgacct ctaccgcgac atggtccggg
     5461 tcggcaccgc gcgctgcggc ttcacgtgtg tctcgccggc ggtctaccgg cggctgcgcg
     5521 ggtcggccgc gggagcgggc accggtgcgg gcaccggtgc gggtgccggt gcgggtgtcg
     5581 acgtgggcgt tgacgtgggt gccggtgcgg gcgcaggtgc ggcaccgctg cgcgacgaac
     5641 tcccgccgcc cgtcgcaccg gagctggtcg gccaggagag cgcgggcgac gtcgtactct
     5701 ccgccatgga ccggccgggc agctggctgc tgcgctgcga ccccgaccac cccgtgctct
     5761 tcgaccaccc cgtcgaccac gtgccgggca tggttgtcct ggaggcgatg cggcaggccg
     5821 cgttcgcccg gtccgggagc cggccgctgc tcgtcggttg cgcgatggag ttcctccggt
     5881 acgccgaact cggcctgccc accgagatcc acgcggcccc cgaaccggcc cgcgccgacg
     5941 gcacggtgcc cgtccgggtc gcagccgcgc agaacggcga catcgttgcc cagggcgtcc
     6001 tgcgggtacg gccggacagc ggggcctgac ggcgatgcgc cccatcctca ccgccttgct
     6061 gcgccggtcc ggcctggcgg tacgcgcctg gctcaggggg agtcggccgg tgatgctccg
     6121 ggtgccgttc gcctatccgg tgcggtcgtc ggcggaggac gatgtgtgag tcgctggtgg
     6181 ggtgggggcg tgcggcgcga ggcgagcgga cagggctctc cgggatggaa gagccctgac
     6241 tccttcggca tggaagggcc ctgacccccc gacggcgccg ggcaccggca gccgcttccg
     6301 agtggacccg gcgccctgtc cgcgatctgt ccgcgatcta tccgcgatct gaacttggcg
     6361 tggctgtgcc gtctgaggcc gtttcggatt tcgtgtatgc gggttccgta tagaggatgt
     6421 cgcgggcctg ttccgcggcg cgggcgatgc tctcggcgac gaagtcgacg aagcgggcga
     6481 tgttctccag acgggtggcg gccggggtgc cggggccgag gagcccgaca ccctgccgtg
     6541 cggtctcggc gagctgggcg ttggaccggg cggcggccat cattccctgg tagaagacgt
     6601 cggtgtcgac gacgtagcgc tcgcggcggc gttcgtcgcg ttcccggcgg atgaggccct
     6661 ggcctgcgag gaacgtgacc gctttggaga cggacgccgg gctgacctgg aggcgctgga
     6721 cgagttcgga cgcggtgagg ctgcccgcgt cggtggtgta gaggcaggtc aggacccggg
     6781 acatcatctt gggcaggccc tgctgcatga ggagggtggt gaacgcctcc tcgtactcgc
     6841 gcacggcctc ggcgtcacgc ccgtgggtcc gggggagcgc cttcgggtcc cggggcgcgg
     6901 tctgcctgcg gcgatgggcg cggcgttcgg tggcgcggtg ggccaggtcg gcacggtagg
     6961 cggtggggcc gccgttgcgc atcacctcac gcgtgatcgt cgaggtgggg cggccgaggc
     7021 gtctggcgat ctccgcgtag gcgagcccgt cggccagtcc cagcgcgatc tgctgacgtt
     7081 cctgctgggt gagcctgcct cccggcatcg cgatctccct tcacgcttcc ctgatgcctc
     7141 caacatagcg ttcactcccc atccaatgca acgagcgaga taattggtgt tgcattagat
     7201 tcaaaaacgt tgcaacgatt tcccatatct gacctgcgat tatggctatc gctcgcaacg
     7261 acttgattgc cagatatatg aatgcaacgt agcgtttctt tcatcagaaa caacggaccg
     7321 aaggagcgca cgatgcagaa gaaccagagc gcgatgcgga agttcgacac ccccgccccg
     7381 atcgccgccg tcctcgacat ccccgcggga cgcgtccagt tgatcgccgc cgaccggggc
     7441 gacaccacgg tcgaggtcct gcccgcggac gcttcgaagg gccgcgacgt gaaggcggcg
     7501 gagcagatcg aggtcgtata cggcgacggc gtcctccggg tcgaggcccc ggcggcgaag
     7561 aaccggctcc tcggtcactc cgggttcatc gaggtgacca tccagctgcc cgccggttcc
     7621 cgcgtcgagg cgaaggcggc cagcgccgag ttccggggcg tcggacggct cggcgacgtc
     7681 accttcgagg gcgcgcaggg ttcggtcaag ctcgacgaga ccgaaggtgc ccgcctcacc
     7741 ctccaggccg gtgacgtctc ggtcggccgc ctgggcggtc ccgcggagat cagcacccag
     7801 aagggcgaca tccacatcgc cgaggccgtg cgcggcacgg tcacactgcg gacccaggcc
     7861 ggtgacgtgt cggtcggtgc cgccgccgga gtctcggcct ccctggacgc cggcacctcc
     7921 tccggccgga tccacaacgc gctcaagaac gccgacggtg cccccggtct gaacatccac
     7981 gtgaccaccg cctgcggcga catcgccgcc cgtagcctct gaaaagccca ggaaggaagc
     8041 caggaaggaa gagggtgtgg tccggcggcg gtgctccttg gagatccgga gggcacagcc
     8101 gccacggtgg ccgtaccgcg gctcccccct ctggagaggc tcgtctccag ggatgcccgt
     8161 caggctcgaa gcgaggtcat gacgaggtcg cggaattccg gccagcggtc caggagatcg
     8221 gcgcggccgg tgagggtgtc cgagacatgc tggacgccga agaagccgga gaccagggcg
     8281 cgggcggcag cctcggggtc gacgccggcc cgcagctcgc cggcctcctt cgcgctggtc
     8341 agcaggtggg cgagaagctg ggtccagccg acgtacggct ggggcaactg ggcgtcgatc
     8401 agggagcgtt cgagctgcag ccgggcgccg gcctggacga cgatgtcggt ggcgaaggcc
     8461 ttcgcggccc cgtcgagcac ccgtaccacg gtgtcgagcg gcgggtgccc ctcggcgagg
     8521 atgccctcca gcagctcggg ccagcgcgag tagtgcgcct cgaccacggc gatggcgagg
     8581 atctccttgt tcgggaagtg gaagtagacg gctcccttgg tcatgccgac gcgcttggcc
     8641 acgtcctgga tggacgtgct tttgaagccc ttctcggcga actcctccgc cgcggcgcgc
     8701 agtacctggg cgcgggtctg cacggcccgt tcctgacgca ggtcgggcag gcggtcctcg
     8761 gtgggggagc ctgtccagcc cgggatcgtg atcttcgagg tcacggtgat cgcgctcctt
     8821 cggtacacgg ccgtgagttc ggatggaggg gtgcgccggt accactttta atataccttc
     8881 ctgtaggtat atttgccacg ggcgcaggtg atggagccat gcgctctctg tgtgagccgt
     8941 gtgtgctcgc ctccgggcgg gccgggggag gggaacgcat gggcatcggc accttgatgg
     9001 acggcgcaca gctcgccgag ggcgggttgc ggacggcgcg ggtggcacag ctggcccggt
     9061 tggcccgggc ctcggcggcc cgcgtggacg cggagcggcg gctgtccccg gaactcgccg
     9121 ccgagatcac cgccgccgga tttccggccc acttcgtgcc gcgccgctgg ggcggggcgg
     9181 ccgccggctt ccggcaactc gtggacgccg tcgcggcact gggcgaggag tgcgtcgcca
     9241 ccgcctggtg cgccgccctc tacgccgcgc acggacggct cgccgcctac ctgcccgaac
     9301 gcggtcagcg cgagctgtgg gacacctccc cggatgtgcg gatcgccgcc gccgtcgtac
     9361 cgcccgccgg acgggccgtc cccgaggggg agggctggcg gatctcgggc gagtggcact
     9421 acgccagcgg tgtcgaccac gccgactggg tgctggtcgc cgtacgggtc gaaggggcgg
     9481 acggcggcgg gtccgaggtc cgctgcttcg cggttccgcg gcgggagttg gtgatcaagg
     9541 acagctggca cagcctcggg ctgcgcggca cgggcagcaa caccgtcgtc gcgggcggcg
     9601 cgggcgtact ggtgccgcgc caccggagtt tcaccctcgc cgatctcgac accgtgcgcg
     9661 cggggtggcc cgctgccacg ccgtgccctt ccggctcgtc gccccgctcc agttcgccgc
     9721 gccgatcctg ggggcnnnnn nnnnnnnnnn nnnnnnnnnn gccgcgcggg gccgtcgcac
     9781 cggaggtgct cgcccgcgcc gacggcgaga tcgaggcggc ccggctgctc ctgggcgccg
     9841 ccgcggaccg ggcggacgcg gccggcctgg ggccggacgc ggtggcccgc aaccagcgcg
     9901 atgccgccct ggccgcggag ttgtgcacga ccgcggtgga ccgcctgttc cgcgccggcg
     9961 gcacccgggt ccacttcgac ggcgaccccc tccaacgcgc ctggcgcgac ctcaccaccg
    10021 gtgcctcaca cgccactctc gccttcggca cggctgcggc ggccttcatg gcggagcgga
    10081 cggcggcctt cgaggcgggg gaagcggccg cctcggggtg gggtgcggat ggtggtacgg
    10141 ccatcccggc cggcacctcc ttccccaccg gagactcccg atgaccaacg cccaccccca
    10201 ccgcgctccc catcagcaca gtgaccccgg ctccaccgcc cgccccacct gctgccccct
    10261 cctccgcccc cacccgcagt cccaccccgc ccgcatagcc ttcttcgacg tggaccacac
    10321 cctcaccacc gtgacgaccc ccgccctctt ctgggagtac tggtccaccg ccgcgcgcac
    10381 cgcgccgttg gccgccgggc gggcgttgcc ggcggcggcc ggggtggcgc gggaggagcg
    10441 caaccgggac tactaccggc tcttcgccgg tgtgcccgcc atggagttgg ggcgggccgg
    10501 ggcccgttgg tacggcggtt tccgggtgac cgcgcagccc ttccttcccg cgccgctcgc
    10561 cgcgctgcgc gcgcaccggg cggccggtga tctggtcgtg ctcgtgtccg gcgcgccgcg
    10621 ggcctgcctg ctgccgctcg cccgcgacct gggagccggg ctggtgctcg gctgtgagca
    10681 gctgacggac aggtggggcc ggctgaccgg cgagatcacg cggccgatga tcggcgccgc
    10741 caaggcggac gcggcggcgg aggtgatggc acggcacggt gccgatccgg cggactgcca
    10801 tgcgtacggg gaccacagct cggatctgcc gctgctccgg ctgtgcgggc accccacggt
    10861 ggtgggggac gatcccgtgc tggccgggca cacggaacgg gatggctggg agcggctccc
    10921 cggcgtcgag agtctcgttc gaatccgcaa ataacatacc gttgagaagg tatgttgttc
    10981 ctgccgggtg acacacagac ggggagggtt agggaggtca gggtgaaac
//
