LOCUS       NZ_CM002285            10839 bp    DNA     linear   CON 29-APR-2024
DEFINITION  Streptomyces roseochromogenus subsp. oscitans DS 12.976 chromosome,
            whole genome shotgun sequence.
ACCESSION   NZ_CM002285
VERSION     NZ_CM002285.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseochromogenus subsp. oscitans DS 12.976
  ORGANISM  Streptomyces roseochromogenus subsp. oscitans DS 12.976
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9663240)
  AUTHORS   Ruckert,C., Kalinowski,J., Heide,L. and Apel,A.K.
  TITLE     Draft Genome Sequence of Streptomyces roseochromogenes subsp.
            oscitans DS 12.976, Producer of the Aminocoumarin Antibiotic
            Clorobiocin
  JOURNAL   Genome Announc 2 (1), e01147-13 (2014)
   PUBMED   24407645
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663240)
  AUTHORS   Ruckert,C., Szczepanowski,R., Heide,L., Kalinowski,J. and Apel,A.K.
  TITLE     Draft genome sequence of Streptomyces roseochromogenes subsp.
            oscitans DS 12.976
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 9663240)
  AUTHORS   Ruckert,C., Szczepanowski,R., Heide,L., Kalinowski,J. and Apel,A.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2013) CeBiTec, Bielefeld University,
            Universitaetsstr. 27, Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.5.3
            Assembly Name                     :: S.roseochromogenes_v1
            Genome Coverage                   :: 20.4x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000497445.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 03:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,098
            CDSs (total)                      :: 9,013
            Genes (coding)                    :: 8,393
            CDSs (with protein)               :: 8,393
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 6 (16S, 23S)
            complete rRNAs                    :: 6, 6 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 620
            CDSs (without protein)            :: 620
            Pseudo Genes (ambiguous residues) :: 0 of 620
            Pseudo Genes (frameshifted)       :: 227 of 620
            Pseudo Genes (incomplete)         :: 534 of 620
            Pseudo Genes (internal stop)      :: 54 of 620
            Pseudo Genes (multiple problems)  :: 178 of 620
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:03:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2105450
            Orig. end                         :: >2116289
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CM002285.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..>10839
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="15"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..>10839
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..>10839
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:>5839](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..>5839
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(70..82)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 22.13"
                     /tool="antismash"
     misc_feature    complement(662..676)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.51"
                     /tool="antismash"
     gene            737..1300
                     /locus_tag="M878_RS59125"
                     /old_locus_tag="M878_09770"
     CDS             737..1300
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146335.1"
                     /locus_tag="M878_RS59125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_09770"
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="WP_245238070.1"
                     /transl_table=11
                     /translation="MRRSWVMAVAAAAAMSVVTGCGDSGGKQGATPSPTVGRQLTVTST
                     AYGDGGTIPRRHTCDGADVSPPLALTAVPPHTASLALLLQDPDAPHGTFTHWLAWGIDP
                     HTRQLAAGEHPQGATEGRNDFKRPGYGGPCPPRGDRPHHYVLTVYATDRRLSLAAGASP
                     ADLLRALSGHTLAAGTLTGRYGRR"
     misc_feature    1339..1352
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.42"
                     /tool="antismash"
     misc_feature    complement(1340..1353)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.04"
                     /tool="antismash"
     misc_feature    complement(1346..1366)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 21.03"
                     /tool="antismash"
     misc_feature    complement(1406..1420)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.0"
                     /tool="antismash"
     gene            1416..1691
                     /locus_tag="M878_RS59130"
                     /old_locus_tag="M878_09775"
     CDS             1416..1691
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575045.1"
                     /locus_tag="M878_RS59130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_09775"
                     /product="hypothetical protein"
                     /protein_id="WP_023545933.1"
                     /transl_table=11
                     /translation="MHRLRRAAVLVAALSTVGLATAGTAYAGGEGGHEHGDMQSSQCKD
                     HDLNLDVLGEVGILNGLLGNALNGEGDPGAQGTHIGSTMGCNNSAD"
     misc_feature    1988..2008
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 24.75"
                     /tool="antismash"
     gene            2026..2883
                     /locus_tag="M878_RS59135"
                     /old_locus_tag="M878_09780"
     CDS             2026..2883
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019063345.1"
                     /locus_tag="M878_RS59135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_09780"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_023545934.1"
                     /transl_table=11
                     /translation="MVIPRLRHITTVCALGAALAACGTADPAGGARPAPAKSPVSASPS
                     ASHPPVLAPGPGGLTPVFEHGPRSLGKTVALTFDADMTSDEGPRAAAGEHFDNPDLIGA
                     LRALKVPATIFMTGRWADEYPTEARSLGHDPLFEVANHSYSHYAFTDDCYGLPTIGAGN
                     MRTDVERAYASLHQAGVPHALPYFRFPGGCYDKRALRTLSGLGLTAVQWDVVSGDAFAT
                     DADAVTQQVLDGVKPGSVVIMHCTLSAAPTTEQVVRAVVPELRRRGYRFVKVSDLIGAS
                     SGHR"
     gene            3080..3796
                     /locus_tag="M878_RS59140"
                     /old_locus_tag="M878_09785"
     CDS             3080..3796
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432056.1"
                     /locus_tag="M878_RS59140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_09785"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_031224600.1"
                     /transl_table=11
                     /translation="MSQVLPSPGDSPFQAERNTRDEAPQFVLPLVVRIERGAPPARTDA
                     LETAARAVLVLLADERAHGDGEWAEAVRSWEDARIRKVVRRARGTEWRRAEALPGITVT
                     GKSAQVRAFPPIPLDGWPKDLAKLQVSGTELEDPEPPVAAGPAQPVLWLNPELEMSAGK
                     AMAQAGHGAQLAWWALSDAERTAWRDAGFALNVRSADPADWNRLTGSGLPVVRDAGFTE
                     IAPGSCTVVADHPALR"
     gene            3806..4444
                     /locus_tag="M878_RS59145"
                     /old_locus_tag="M878_09790"
     CDS             3806..4444
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073958.1"
                     /locus_tag="M878_RS59145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_09790"
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_023545936.1"
                     /transl_table=11
                     /translation="MTRVDGRVERGNRTRRLVLRRTVDIASVEGLEALTVGRLAAELEL
                     SKSGVFALFGSKQELQLATVQEAARIFTERVLRPAAESPAGAGRVWRLCELWLEYSRGR
                     VFPGGCFFYGAMAEFDARSGPVHDAVVRAQRDWSAHVERTIREARAAGELRADTDVPQL
                     AFELVALLETANALSVLHDEETAYRRARVGIAARLRAAAVDGVPLLQVP"
     misc_feature    complement(4484..4493)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.0"
                     /tool="antismash"
     misc_feature    4511..4520
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.12"
                     /tool="antismash"
     gene            5001..>5839
                     /locus_tag="M878_RS99715"
                     /old_locus_tag="M878_09795"
     CDS             5001..>5839
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016327585.1"
                     /locus_tag="M878_RS99715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_09795"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_023545937.1"
                     /sec_met_domain="DUF692 (E-value: 1.6e-94, bitscore: 315.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRLGTGIGWRPEIADAVERMPGIDWVEVVAENVCPGHLPESLLR
                     LRERGVTVIPHGVSLGLGDADRPDAGRLTALAERAEALGSPLVTEHIAFVRAGGPLTAS
                     PRLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALFSWPGEELTEGQFLS
                     ELVERTGVRLLIDVANLHTNHVNRGEDPGQALAELPLEALAYVHVAGGFERDGVWHDSH
                     AHPVPRPVLDILTDLASRTAPPGVLLERDENFPEPAALERELTLIREAVRAGRERAEG"
     assembly_gap    5840..5864
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     gene            <5865..6301
                     /locus_tag="M878_RS99720"
                     /pseudo=""
     CDS             <5869..6301
                     /codon_start=3
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983658.1"
                     /locus_tag="M878_RS99720"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="endonuclease"
                     /pseudo=""
                     /transl_table=11
                     /translation="VAVLTRPAVGARERVGVAQAAVLSSLVAGTPVPEGFDRVRMRVQA
                     RALTAKRADVVAKVAPELPVILGERYRPAFLDYARERPMSGGYRQDALTFAAHLLGTGR
                     PEDPGALRALRQWWLDRAGPVPRSGGRVARATRKVLRHRG"
     gene            6518..7291
                     /locus_tag="M878_RS59155"
                     /old_locus_tag="M878_09805"
     CDS             6518..7291
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997027.1"
                     /locus_tag="M878_RS59155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_09805"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_023545938.1"
                     /transl_table=11
                     /translation="MRPRPPIKGRGIFSGTGLVIAALAATASALLYPLWSYADRQSPAA
                     GATVLSTQTVTTSYGPLSTQDQDFITKVRLADLWELPAGELAERKGTTAAVRTAGQHLV
                     DGHTSLDAHVRTVATQLGVPLPNEPNTQQNQWLDTLGAAQGEDFDRQFAGILRLAHARV
                     FTLVAQVRADTQNSLVRDLADDANATVLDHIKVMEATGYVDFAALAKELAASPTPVPTP
                     PAVAPSAAVPVTPEPGESYSMPPATTSPPPQTSSP"
     misc_feature    complement(7333..7347)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.5"
                     /tool="antismash"
     gene            7421..8200
                     /locus_tag="M878_RS59160"
                     /old_locus_tag="M878_09810"
     CDS             7421..8200
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675656.1"
                     /locus_tag="M878_RS59160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_09810"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_023545939.1"
                     /transl_table=11
                     /translation="MFWVLLLLLAWAFAGTACTRLCLTAVRAAALDTDATPAAPGHDLT
                     LYEAAFLSGGPARVADVTLVSMARQRRLLLAHTGWATVVDPRGRDEIEQSVIGAIGPEG
                     QSRIAPVRARAAGAEAVRRLADGLVRAGLAVPEGVGTTVGAAVRQVRAAALAVAVLGLT
                     ALLLPIQTGTPRLLVGLWFALPLTLSLSCLVIARFEVHPYSRWASPAGQRLLGALVRRP
                     AGDERSFLTSVAVRGIRAIGEPQLRAAFAHRDQLWRE"
     misc_feature    8308..8322
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.6"
                     /tool="antismash"
     assembly_gap    8426..8903
                     /estimated_length=478
                     /gap_type="within scaffold"
                     /linkage_evidence="unspecified"
     gene            <8904..10442
                     /locus_tag="M878_RS59165"
                     /old_locus_tag="M878_09820"
     CDS             <8904..10442
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (78-450). E-value:
                     1.1e-17. Score: 56.4. Matches aSDomain:
                     nrpspksdomains_M878_RS59165_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675655.1"
                     /locus_tag="M878_RS59165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M878_09820"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_023545940.1"
                     /sec_met_domain="PF00561 (E-value: 2.6e-21, bitscore: 75.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LRGTEVAVARARAAGVDFGACAAADVPKAARNVRCGTVTVPLDYA
                     HPNGTQITLTVSRTPATQKDPRNSKRKVPRQGALFYNPGGPGASSMLFPLVGAIPEWKR
                     IAAAYDLIGYAPRGVGRSAPLSCETPKRFVTGPTASPYHPSESFKRQRIAQAKAYARGC
                     ALHAGKTLPFYTSLNNARDLDVLRAALGEDRLTFYGASYGTYFGALYATMFPTHVRRMV
                     LDSAVNPDPQKIWYRNNLDQSAAFEERWADFRDWIARHDDVYRLGNTAARVQRSYDIAR
                     ERLAGKAAGGKVGPGQLQSALLKAGYSDSYWPSRAQALSAYLHGDSKPLVQMAAPSPKE
                     AADAENTNAVYTAVECNDAPWPTDWKVWDRDNTRLARVAPFETWDNAWMNLPCAYWPAP
                     RQQPLDVRTGQGELPPVMILAAERDAAAPYAGSLEMNRRLADSVLVTERDAGTHGIGAG
                     PNKCVNGYLDSYLLEGRFPVRRASCAPREEPVASGRSAGKKPQADSRRGRTGRQ"
     aSDomain        9141..10256
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_M878_RS59165_Abhydrolase_1.1"
                     /evalue="1.10E-17"
                     /label="M878_RS59165_Abhydrolase_1.1"
                     /locus_tag="M878_RS59165"
                     /protein_end="450"
                     /protein_start="78"
                     /score="56.4"
                     /tool="antismash"
                     /translation="LFYNPGGPGASSMLFPLVGAIPEWKRIAAAYDLIGYAPRGVGRSA
                     PLSCETPKRFVTGPTASPYHPSESFKRQRIAQAKAYARGCALHAGKTLPFYTSLNNARD
                     LDVLRAALGEDRLTFYGASYGTYFGALYATMFPTHVRRMVLDSAVNPDPQKIWYRNNLD
                     QSAAFEERWADFRDWIARHDDVYRLGNTAARVQRSYDIARERLAGKAAGGKVGPGQLQS
                     ALLKAGYSDSYWPSRAQALSAYLHGDSKPLVQMAAPSPKEAADAENTNAVYTAVECNDA
                     PWPTDWKVWDRDNTRLARVAPFETWDNAWMNLPCAYWPAPRQQPLDVRTGQGELPPVMI
                     LAAERDAAAPYAGSLEMNRRLADSVLVTERDA"
ORIGIN
        1 tggaccggct gcagaaggtc aacatgcaga tcgtgacgat gccgatcccg gcccaggagg
       61 gcatcacgcg cgacaacgtc accgtccgtg tggacgcggt cgtctacttc agggtgatcg
      121 acgcggagag cgccctggtg aaggtcgagg actacaagtt cgcggtgtcg cagatggccc
      181 agacgtccct caggtcgatc atcggcaaga gcgacctgga cgatctgctc tccaaccgcg
      241 agaagctcaa ccaggggctg gagctgatga tcgacagccc ggccgtcggc tggggcatcc
      301 aggtcgaccg ggtggagatc aaggacgtct ccctgccgga cacgatgaag cggtccatgg
      361 cccgccaggc agaggccgac cgggagcgcc gggcccggat catcaacgcc gacgccgagc
      421 tccaggcctc caagaagctg gccgaggccg cgcagcagat ggccgacacc cccgccgcgc
      481 tgcagctgcg gctgctccag acggtgatgg cggtctccgc cgagaagaac tccacgctgg
      541 tcctgcccat cccggtggag ctgctgcact tcctggagcg aggcaaccag cagcacgtgc
      601 cgaacggtga cggagaacgc ccgtcggact agggtccgtc cggcgggtca ggtcgcggga
      661 aatcgacggc gcggatgggc gtgccggccc ccgcgtctgc gacaggatcc gccggacatg
      721 ccctagcgtg ccgggcatgc gcaggagttg ggtgatggcc gtggccgcgg cggccgcgat
      781 gagcgtcgta acgggctgcg gggacagcgg cggcaaacag ggcgccacgc cctcccccac
      841 cgtcggccgg cagctgaccg tcaccagcac cgcgtacggc gacggcggca ccatcccgcg
      901 ccgccacacc tgcgacggcg cggacgtgtc gcccccgttg gccctcaccg ccgtaccccc
      961 gcacacggcc tcgctggccc tgcttctgca ggaccccgac gccccgcacg gcacgttcac
     1021 gcactggctg gcgtggggca tcgacccgca cacgaggcaa ctggccgccg gggaacatcc
     1081 gcagggagcg accgagggcc gcaacgactt caagaggccc ggctacggcg gcccctgccc
     1141 accgcgcgga gaccgcccgc accactacgt cctcacggtc tacgccaccg accgccgact
     1201 gtcgctcgcg gcaggggcct cgcctgccga cctgctgcgg gcgctgtccg gtcacaccct
     1261 cgccgcaggc acgctgaccg gccgatacgg gcgcagatag tcccacgaat tacggcgcgt
     1321 cgctgtccgt tttgggcgat tttaatacta atctccgtga ctgtaagagg tcgatctgtt
     1381 gcagaccgct tccgctctca caccatggag atgacatgca cagacttcgc agggccgccg
     1441 ttctggtcgc agctctcagc accgtcggac tcgcaaccgc cggcaccgcg tacgccggcg
     1501 gcgaaggcgg gcacgagcac ggcgacatgc agagctccca gtgtaaggac cacgacctga
     1561 atctcgacgt cctcggcgag gtcgggatcc tcaacggcct gctcggcaac gcgctcaacg
     1621 gcgagggtga ccccggtgca cagggcaccc acatcggctc gaccatgggc tgcaacaaca
     1681 gcgccgactg agcggcccac tcagatgccc ttgacggcgg aagacgatgg tgggcctggc
     1741 gtccggtggg ccgggcggcc tccgcaccgc cggcagggga atgcgtggcg acctcacgcg
     1801 cgcgcggacg agtccgtgag cgagttccgt aggtgcgcgt gaggtcgcca cgccgtcgac
     1861 ggcagcggag tgccgatacg cgctccgctg ccgcttccgt ttgagcaccg gcgaacaccc
     1921 ggccgccgga gcacaggaat ccgcgagaac gccgccgtgc gggtgaccgt gacgcggccc
     1981 gccgaacata tcgacatata gacgatcatt cagtcctatt ttcatatggt gatcccacga
     2041 ctacgccata tcaccaccgt ctgtgccctg ggcgcggctc tcgccgcctg cggtaccgcg
     2101 gaccccgcgg gcggtgcccg cccggccccg gccaagtccc ccgtctccgc ctccccgtcc
     2161 gcctcgcacc cgcccgtgct ggcccccggc cccggcggcc tgaccccggt cttcgagcac
     2221 ggcccgcgct ccctgggcaa gaccgtcgcg ctgaccttcg acgccgacat gacctccgac
     2281 gagggtcccc gtgccgccgc cggcgagcac ttcgacaacc cggatctcat cggcgccctg
     2341 cgggcgctga aggtgccggc gacgatcttc atgaccggcc gctgggccga cgagtacccg
     2401 accgaggccc gcagcctcgg ccacgacccg ctgttcgagg tcgccaacca ctcctacagc
     2461 cattacgcgt tcaccgacga ctgctacggc ctgccgacga tcggagccgg aaacatgcgg
     2521 acggatgtgg agcgggcgta cgcctccctg catcaggcgg gcgtgccgca cgcgctgccc
     2581 tacttccgct ttcccggcgg ctgttacgac aagcgggcgc tgcgcacgct gagcgggctc
     2641 gggttgaccg cggtgcagtg ggacgtggtg agcggcgacg cgttcgccac ggacgccgac
     2701 gccgtcaccc agcaggtgct ggacggggtg aagcccggct cggtggtcat catgcactgc
     2761 accctcagcg ccgccccgac caccgagcag gtcgtccgcg cggtcgtccc ggaactgcgc
     2821 cgacgcggct accggttcgt gaaggtctcc gacctgatcg gcgcgagcag cggacaccgc
     2881 tgagcggccc gggctggaga catgagcgac gaggacgaca ggacctactg aacgatcgtc
     2941 gacgccacac agccaccgaa ggccgccggc ccgtctccga accggtgacc aagccgttgg
     3001 cggcggtagc ctcatatcag ccgttggcgg cggtagccgc atatcagaca cagccctgcg
     3061 ccccttaggg ttggccggtg tgagccaagt tttgccgtcc cccggagaca gccccttcca
     3121 ggccgagcgg aatacgcgcg acgaagcacc ccagttcgtg ctgcccctcg tcgtacggat
     3181 cgagcggggt gcgccgcccg cgcgtaccga tgcgctggag acggctgccc gggcggtgct
     3241 cgtgctgctc gcggacgagc gggcgcacgg ggacggtgag tgggccgagg ccgtgcggag
     3301 ctgggaggac gcgcggatcc ggaaggtcgt gcggcgggcc cggggcaccg agtggcggcg
     3361 ggccgaggcg ctgcccggga tcacggtgac cggaaagtcc gcacaggtgc gggccttccc
     3421 gccgatcccg ctcgacggct ggcccaagga cctggccaag ctgcaggtgt cgggcaccga
     3481 gctggaggac ccggagccgc ccgtcgccgc cggcccggcg cagcccgtgc tgtggctgaa
     3541 cccggagctg gagatgtcgg ccgggaaggc gatggcccag gccgggcacg gcgcccagct
     3601 ggcctggtgg gcgctgtccg acgcggagcg caccgcctgg cgggacgcgg gtttcgcgct
     3661 gaacgtccgc agcgccgacc ccgccgactg gaaccggctg accggcagcg ggctgccggt
     3721 ggtacgggac gccggcttca cggagatcgc gccgggctcc tgcacggtcg tcgccgacca
     3781 ccccgcgctg cgctaggaga gacggatgac gcgggtcgac gggcgggtcg agcggggcaa
     3841 tcgcacccgt cggctggtgc tgcgccgcac ggtggacatc gcctcggtcg agggtctgga
     3901 ggcgctcacc gtcggccggc tcgccgccga gctggagctg agcaagagcg gggtgttcgc
     3961 gctcttcggt tccaagcagg aactgcagct ggccacggtg caggaggcgg cgcggatctt
     4021 caccgagcgg gtgctccgtc ctgccgccga gagcccggca ggcgccggcc gggtgtggcg
     4081 gctgtgcgag ctgtggctgg agtactcgcg cggccgggtc ttcccgggcg gctgcttctt
     4141 ctacggcgcc atggccgagt tcgacgcgcg gtccggcccc gtgcacgacg ccgtggtccg
     4201 cgcgcagcgc gactggtcgg cccatgtcga gcgcacgatc cgggaggcgc gtgcggcggg
     4261 tgagctgcgc gccgacacgg atgtcccgca actcgccttc gaactggtgg cattgctgga
     4321 gacggcgaac gccctgtcgg tcctgcacga cgaggagacc gcgtaccgca gggcccgggt
     4381 gggtatcgcg gcacgactgc gcgctgctgc cgtggacggg gtcccgctcc tccaagtccc
     4441 ctgacctggc tgccccctgg gagcacgact ggttgcacag ctgccaaaaa ataagcacga
     4501 tcgttcgctt tgttttgtgc tcgggccatg actgcgaccg gagcggactt cgtggaactc
     4561 gatgaccgcg cagcacctgg ggttcgccgc ggcggcgcgc ggagcggggc gggagccgga
     4621 tcactgacgg gaggacgagg agatgagcga gcccgccggg gcacaccggt cggccgccgt
     4681 cacagtcctc tcggccttcg cggaaccggg cgtgagggaa cgggagttcg cgccgccgaa
     4741 tctgggcggc gttcggggtg gccgaggggt cggcttccac ttcgtcgact atgtggtgca
     4801 tgcctgggac gtcgccgcca ccctgggcac cacggtgaca ttccccgagc acgtcctcgt
     4861 ctccgcgccg gccgtggccg gcacggggct gaagctcaaa tgttgctctg aacacgcccc
     4921 ggaggtgatt cgggtccggc cgtcggggcc atacgtccgt cattcgcggg agggactcgt
     4981 cgttcgaagg agggggagcc atgcgccgac tgggcacggg gatcggctgg cggccggaga
     5041 tcgcggacgc cgtggagcgc atgccgggca tcgactgggt ggaggtcgtg gcggagaacg
     5101 tctgccccgg ccaccttccg gagtcgctgc tacggctgcg cgagcgcggg gtgacggtga
     5161 tcccgcacgg cgtctccctg ggcctgggcg acgccgaccg ccccgatgcg ggccgcctga
     5221 cggcgctggc ggagcgcgcg gaggccctcg gctcccccct ggtcaccgag cacatcgcgt
     5281 tcgtccgcgc gggcggcccg ctcaccgcct ccccccggct ggaggccggg catctgctgc
     5341 cggtgccgcg caccagggac gccctggacg tgctgtgcga gaacgtgcgc atcgcacagg
     5401 acgcccttcc ggtgccgctg gccctggaga acatcgccgc gctgttctcc tggccgggcg
     5461 aggagctgac cgaggggcag ttcctgtccg agctggtcga gcgcaccggc gtacgactgc
     5521 tgatcgacgt ggccaacctg cacaccaatc atgtcaaccg gggcgaggac ccggggcagg
     5581 cgctcgccga gctgccgctg gaggcactcg cctacgtcca tgtcgcgggc ggcttcgagc
     5641 gtgacggcgt ctggcacgac agccatgcgc accccgtgcc gcgcccggtg ctcgacatcc
     5701 tgaccgacct cgcctcccgt accgccccgc ccggtgtcct gctggaacgg gacgagaact
     5761 tccccgaacc ggccgcgctg gagcgggagt tgacgctgat ccgggaggcg gtgcgggctg
     5821 gacgcgagcg cgccgagggn nnnnnnnnnn nnnnnnnnnn nnnnctgtcg ccgtgctcac
     5881 ccgtccggcg gtcggcgcgc gggagcgggt cggtgtggcc caggccgcgg tgctgtcctc
     5941 gctggtggcc gggacaccgg tgccggaggg gttcgaccgg gtgcggatga gggtacaggc
     6001 gcgggcgctc acggcgaagc gggcggacgt ggtggcgaag gtggcaccgg agctgccggt
     6061 gatcctcggc gagaggtacc ggccggcgtt cctcgactac gcccgggagc ggccgatgag
     6121 cgggggctac cggcaggacg ccctgacctt cgccgcgcac ctgctgggga ccggccggcc
     6181 ggaggacccg ggggccctgc gtgcgctgcg gcagtggtgg ctggaccgag cgggcccggt
     6241 cccgaggtcc ggcgggcggg tggctcgggc gacgcggaag gtgctgcggc atcggggctg
     6301 aggggtcgct caccggcgcc ggccgggtgc cgctgcgggc agcccctagg ggcgcgggga
     6361 actgcgcgac cagccacgac gcacccgcag ccgccgacgc accgtaacct cccgaaccct
     6421 ctgcgtaccg acccgagccc cgcagggaac ccgcagtaat atgccgtccg caccaccccc
     6481 caggcacact cccgtgcggt gcgctacagg aggcgccatg cgaccccgac ccccgatcaa
     6541 gggccgaggc atattcagcg gcacaggcct cgtcatcgcg gccctcgcgg cgaccgcgtc
     6601 ggcgctgctc tacccgctct ggtcgtacgc cgaccgccag agcccggcgg ccggcgccac
     6661 cgtgctgagc acacagaccg tgaccacttc ctacggcccc ctgtccacgc aggaccagga
     6721 cttcatcacc aaagtgcggc tggcggatct ctgggagctg cccgccggcg agctggcgga
     6781 gcgcaagggg accacggcgg ccgtacggac ggcgggacag cacctggtcg acgggcacac
     6841 ctcgctggac gcccatgtcc gcaccgtcgc cacccagctc ggcgtaccgc tgccgaacga
     6901 gccgaacacg cagcagaacc agtggctcga caccctgggc gcggcgcagg gcgaggactt
     6961 cgaccgccag ttcgccggca tcctgcggct cgcgcacgcc cgggtgttca cgctggtcgc
     7021 gcaggtccgg gccgacaccc agaactccct ggtccgtgat ctcgccgacg acgccaacgc
     7081 gaccgtcctg gaccacatca aggtcatgga ggcgacgggg tacgtcgact tcgccgcgct
     7141 cgcgaaggag ctggccgcct cccccacccc ggtgccgacc ccgccggcgg tcgccccgag
     7201 cgccgccgtg cccgtcacgc ccgagccggg cgagtcgtac tccatgccgc cggccaccac
     7261 cagcccgccg ccgcagacga gcagcccctg acgcaccgcc cctgcctggc ctgacccggc
     7321 ctgaccttgg ggtgaaacgg aatgtcacag acggctgaca ctgtgtccgg atgatgaaca
     7381 aggcatggcg ttcacccagg acctggcata gaaacttgtc atgttctggg tcctcctcct
     7441 gctcctggcc tgggctttcg ccggtacggc gtgcacccgg ctgtgcctga cggccgtccg
     7501 cgcggccgct ctggacacgg atgccacgcc ggcggccccg gggcacgacc tgacgctgta
     7561 cgaggcggcg ttcctctccg gcggcccagc ccgggtcgct gatgtgaccc tcgtctccat
     7621 ggcccgccag cggcgcctgc tgctggcgca caccggctgg gccacggtgg tggacccacg
     7681 ggggcgcgac gagatcgagc agtccgtcat aggggccata ggcccggaag ggcagtcacg
     7741 gatcgcgccc gtgcgggcgc gggcggccgg cgccgaggcg gtgcgacggc tcgccgacgg
     7801 gctggtgcgc gccggtctcg ccgtccccga gggcgtcggt acgaccgtgg gggccgccgt
     7861 gcgccaggtg cgcgccgccg cgctggccgt ggcggtcctc ggcctgaccg cgctgctgct
     7921 gcccatccag accgggacgc cgcggctgct ggtcggcctg tggttcgccc tgcccctcac
     7981 gctgtccctg agctgtctgg tcatcgcccg gttcgaggtg cacccgtact cgcgctgggc
     8041 ctctccggcc gggcagcggc tgctgggcgc gctcgtgcgc aggccggccg gcgacgagcg
     8101 gtcgttcctc acctcggtcg ccgtacgcgg aatccgcgcg atcggcgagc cgcagctgcg
     8161 cgccgccttc gcccaccgcg accagctctg gcgggagtga cgcgaccagt tccggcggga
     8221 gtgaggggcg gcttcggggg cgcatagagg cattgacgcc gacaccggcc ggacggtgct
     8281 tgccttcacc gacgggcgca acgaaatatc ccttctgttg atcgccgggc gtggcagcca
     8341 tgccatcgcc gccgcatcga agggacacgc gatgagagct gccgccctct actcggccgc
     8401 cgggaccctg ctcctgacca ccctgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnctgcgcg gcaccgaggt ggccgtggcc cgcgcccgcg
     8941 cggccggcgt cgacttcggc gcgtgtgccg ccgccgacgt accgaaggcc gcgcgcaacg
     9001 tgcggtgcgg cacggtgacc gtcccgctcg actacgccca ccccaacggc acgcagatca
     9061 cactgaccgt cagccggacc ccggcgaccc agaaggaccc gcgcaacagc aagcggaagg
     9121 tcccccggca gggcgccctc ttctacaacc cgggcggccc cggcgcgtcc agcatgctgt
     9181 tcccgctggt cggcgcgatc ccggagtgga agcggatcgc ggccgcctac gacctgatcg
     9241 gctacgcccc gcgcggcgtg ggccgttcgg ccccgctgtc ctgcgagacc ccgaagcgct
     9301 tcgtcacggg gcccaccgcg tcgccgtacc acccgtcgga gtcgttcaag cggcagcgca
     9361 tcgcccaggc caaggcgtac gcccgcggct gcgccctgca cgccgggaag acgcttccct
     9421 tctacacctc gctgaacaac gcccgggacc tggacgtcct gcgggccgcg ctgggcgagg
     9481 accggctgac gttctacggg gcgtcgtacg gcacctactt cggcgcgctg tacgcgacga
     9541 tgttccccac gcacgtacgg cgcatggtgc tggactcggc ggtgaacccg gacccgcaga
     9601 agatctggta ccgcaacaac ctcgaccagt cggcggcgtt cgaggagcgc tgggcggact
     9661 tccgggactg gatcgcccgg cacgacgacg tctaccggct cggcaacacc gccgcgaggg
     9721 tgcagcgcag ctacgacatc gcgcgggagc ggctggccgg caaggcggcc ggcgggaagg
     9781 tcggacccgg ccagctgcag agcgcgctcc tgaaggccgg gtattccgac agctactggc
     9841 cgagccgggc ccaggccctg tcggcgtatc tgcacggcga ctcgaagccg ctggtgcaga
     9901 tggctgcgcc gagcccgaag gaggccgcgg acgcggagaa caccaacgcg gtctacacgg
     9961 ccgtggagtg caacgacgcg ccctggccga cggactggaa ggtgtgggac cgggacaaca
    10021 cccggctcgc ccgggtggcg cccttcgaga cctgggacaa cgcgtggatg aacctgccgt
    10081 gcgcctactg gccggcacct cgccagcagc cgctggacgt gcggaccggg cagggtgagc
    10141 tgccgccggt gatgatcctg gcggctgagc gggacgcggc ggcgccgtac gcggggtcgc
    10201 tggagatgaa ccggcggctg gccgactcgg tgctggtgac cgagcgggac gcggggacgc
    10261 acggcatcgg ggccggaccc aacaagtgcg tcaacgggta cctcgacagc tatctgctgg
    10321 agggccggtt ccccgtgcgg cgcgcgtcgt gcgcgccgcg cgaggaaccg gttgcctccg
    10381 gccgttcagc cggaaagaag ccccaggcag actcgcggcg aggcaggacc ggccggcagt
    10441 aggcgctccc cgccccccgg agcgctgtca ggccaggcct gccaccaggt ccgcgatggt
    10501 cttgcggcgg cccgtgtaga acgggatttc ctcgcggacg tgcctgcggg cctcggaggc
    10561 gcgcaggtgg cgcatgaggt cgacgatgcg gtacagctcg tcggcctcga aggccaggat
    10621 ccactcgtag tcgccgaggg agaacgaggc gaccgtgttg gcgcgcacgt ccgggaagcc
    10681 gcgggccatc ttgccgtggt cggcgagcat gcggcggcgg tcctcgtcgg gcagcagata
    10741 ccagtcgtag gagcgcacga aggggtagac gctcacgtag ttgcggggcg tctcgtcggc
    10801 gaggaacgcc gggatgtgcg agcggttgaa ctcggcggg
//
