LOCUS       NZ_CM002273            11314 bp    DNA     linear   CON 11-AUG-2024
DEFINITION  Streptomyces sp. PVA_94-07 strain PVA 94-07 chromosome, whole genome
            shotgun sequence.
ACCESSION   NZ_CM002273
VERSION     NZ_CM002273.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. PVA_94-07
  ORGANISM  Streptomyces sp. PVA_94-07
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7007517)
  AUTHORS   Ian,E., Sekurova,O.N., Malko,D.B., Ruckert,C., Borisova,M.E.,
            Albersmeier,A., Kalinowski,J., Gelfand,M.S. and Zotchev,S.B.
  TITLE     Draft sequence of Streptomyces sp. PVA 94-07, a marine actinomycete
            isolated from the sponge Phakellia ventilabrum collected in the
            Trondheim fjord (Norway)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7007517)
  AUTHORS   Ian,E., Sekurova,O.N., Malko,D.B., Ruckert,C., Borisova,M.E.,
            Albersmeier,A., Kalinowski,J., Gelfand,M.S. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2013) CeBiTec, Bielefeld University,
            Universitaetsstr. 27, Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Assembly Name                     :: PVA94-07.23
            Genome Coverage                   :: 158.81x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000495755.1-RS_2024_08_11
            Annotation Date                   :: 08/11/2024 02:29:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,173
            CDSs (total)                      :: 6,079
            Genes (coding)                    :: 5,859
            CDSs (with protein)               :: 5,859
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 220
            CDSs (without protein)            :: 220
            Pseudo Genes (ambiguous residues) :: 0 of 220
            Pseudo Genes (frameshifted)       :: 97 of 220
            Pseudo Genes (incomplete)         :: 166 of 220
            Pseudo Genes (internal stop)      :: 15 of 220
            Pseudo Genes (multiple problems)  :: 55 of 220
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:06:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1167100
            Orig. end                         :: 1178414
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CM002273.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Department of Biotechnology,
            Norwegian University of Science and Technology, 7491 Trondheim,
            Norway.
FEATURES             Location/Qualifiers
     region          1..11314
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11314
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11314
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6314](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6314
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(166..1037)
                     /gene="sigJ"
                     /locus_tag="B590_RS04695"
                     /pseudo=""
     CDS             complement(166..1037)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="sigJ"
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951597.1"
                     /locus_tag="B590_RS04695"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="RNA polymerase sigma factor SigJ"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAYRLLGSLAEAEDAVQEAYARWYALPDPRRDEIASPGAWLTTVT
                     GRVCLDVLGSARARRERYVGAWLPDPLPGHPGRPGRAATPPTSWCWTSPSPWPSSSSWR
                     R"
     gene            1207..1584
                     /locus_tag="B590_RS04700"
                     /old_locus_tag="B590_04775"
     CDS             1207..1584
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641248.1"
                     /locus_tag="B590_RS04700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B590_04775"
                     /product="hypothetical protein"
                     /protein_id="WP_023416412.1"
                     /transl_table=11
                     /translation="MTTTQATPRTTVRTRFSLLRTALALQALTIFVQAVSAGLLLSTDY
                     GELLHGVGARVMYGASMLYLLAAVLAWKPGGGSPKAVWHASGFLVLASVQVVSGIAHLS
                     SLHVPLGVLMFGASLAALARR"
     gene            1742..3106
                     /locus_tag="B590_RS04705"
                     /old_locus_tag="B590_04780"
     CDS             1742..3106
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951595.1"
                     /locus_tag="B590_RS04705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B590_04780"
                     /product="PhoX family protein"
                     /protein_id="WP_023416413.1"
                     /transl_table=11
                     /translation="MSATRRQVLARTGAVAGIAFTGSLSELFAGTAAAKGMGKAGYGPL
                     VPDPKGLLDLPKGFRYRVLSREGDRMLSGEGQVPSNHDGMAAFGGRRGQVHLVRNHENR
                     PTAPIRVPPVRGLTYDPEGMGGCTALTLDPAHRVLGERVAIAGTSTNCAGGPTPWGTWL
                     TCEETEDKAGTNGYTKDHGFIFEVDPVDPHRSGAVPLKAMGRFQHEAIAIDPRHGIVYE
                     TEDAFERPFGLFYRFLPEKPRGGLGSLRAGGALQAMRVPGVPDLSAIQETGASFDRVEW
                     VDVPDPLAEETPIRFQDFGPKGITHAQKLEGCYWGGRSVYFVSSFARSSEGSAADHYGQ
                     IWRYDPDRRRLTLVIVFGPDTDVQLPGEEPDNICLAPSGGLMVAEDGEGAQHVYGVTTR
                     GEVYPMARGRQNTGTPEEPAWGEFAGVCFSPDYDTMFVNCYTPGTTFAVTGPWHR"
     gene            3276..4145
                     /locus_tag="B590_RS04710"
                     /old_locus_tag="B590_04785"
     CDS             3276..4145
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951592.1"
                     /locus_tag="B590_RS04710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B590_04785"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_037970204.1"
                     /transl_table=11
                     /translation="MVRKAKLRTGTALGALMAVGAVAGCGHSSPDPHDTAPGPRASHSA
                     SPAAPPAPPTLAPGPGGLAPVFANGPRNDGKTVALTFDADMTADQGERAAGGERFDHPE
                     LIASLRRLKVPATVFMTGRWAEEYPDQARAIGADPLFEIANHSYSHHAFTGDCYGLPRV
                     AESEMRADVERAYTAFRTAGVKKAMPYFRFPGGCYDDTALREVSAAGVTAVQWDVVSGD
                     AFATDAGAVAQQVLDGVRPGSVVVMHCTRSAAPATERALRRIVPALREQGYRFVKVSEL
                     IGAASRKG"
     gene            4176..4889
                     /locus_tag="B590_RS04715"
                     /old_locus_tag="B590_04790"
     CDS             4176..4889
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018894649.1"
                     /locus_tag="B590_RS04715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B590_04790"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_023416415.1"
                     /transl_table=11
                     /translation="MTSEPLPDSPFRDERTARDEAPQFVLPLVVRLERAEPPALTDALQ
                     TAARAVLTLLSDPRSEGDGVWAEAVRDWQDARIRKVVRRARGAEWRKAAALPGITVEDG
                     GAEVRVYPPVPLDGWPRELAKLQVSGTELEDPVEPGEPAAGVPVLWLNPDVPMSAGKAM
                     AQAGHGAQLAWWQLSGAERNAWREQGFPLAVRTAGAERWKELVESGLPVVRDAGFTEIA
                     PGSCTVVAEHPALVG"
     gene            5001..6314
                     /locus_tag="B590_RS04720"
                     /old_locus_tag="B590_04795"
     CDS             5001..6314
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641256.1"
                     /locus_tag="B590_RS04720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B590_04795"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_023416416.1"
                     /sec_met_domain="DUF692 (E-value: 3.3e-94, bitscore: 313.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPAIAEAVEAMAGIEWVEVVAENVCPGHLPDSLRR
                     LRERGVAVVPHGVGLGLGGAERPDPVRLAALAERAEALDSPLVTEHIAFVRAGGERTAS
                     RGLEAGHLLPVPRTREGLAVLCENVRIAQDQLPVPLAVENIAALFSWPGEELTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNQGEDPAAALDALPLEALAYVHVAGGFERDGVWHDSH
                     AHPVPDPVLAVLAELASRVRPPGVLLERDERFPSAAGLEAEVAKIRRTVAAAGRDRVSV
                     GAGARPGSVPASGSPVADEAARTRLALGQTALLSALVAGTPAPEGFDRGRLRVQNRMLT
                     AKRGSVVASLAPELERILGDTYRPRFLRYAAGRPLTGGYRADALAFALHTLREEPPAER
                     AVRRALRDWWIERSGRASLVGRPWARWARVARFVLLGR"
     misc_feature    6437..6455
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.23"
                     /tool="antismash"
     gene            6438..8096
                     /locus_tag="B590_RS04725"
                     /old_locus_tag="B590_04800"
     CDS             6438..8096
                     /NRPS_PKS="Domain: Abhydrolase_1 (105-479). E-value:
                     1.1e-21. Score: 69.5. Matches aSDomain:
                     nrpspksdomains_B590_RS04725_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404759.1"
                     /locus_tag="B590_RS04725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B590_04800"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_023416417.1"
                     /sec_met_domain="PF00561 (E-value: 1.6e-24, bitscore: 85.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAPLILTAAGALLLTPLAAAPQAPAAGAPAPSGGPEVRGVALAA
                     ERAARTPIAFGPCPDEEGLPRSLECGTVRVPLDYARPDGEQIDLTVSRAKAAGPKGERQ
                     GALVFNPGGPGSSGMLFPLVGIFPEWKRLASVYDLVGYAPRGVGRSAPLSCRDPREKPD
                     GSWQAPEHPTEKQKAGRRAEAEAYARGCAERGGKRLRHIHSLNNARDLDVVRAGLGERK
                     LTFMGASYGSYFGALYAQLFPSHVRRMVFDSAVDPSPSRVWYRNNMAQSAAFEKRWADF
                     RRWIASHDAAYGMGTDAAQVLAGYRRAREKLAAEPAGGTVGPAQLQAVMLRAQYYEDYW
                     PSHAEALAAYLKGDPQPLVNLATPLEEGRVEAENARAVYTAVECNDAPWPTSWETWDRD
                     HTRLARTAPFETWENAWMNLPCAYWKEPRQTPMDVRVAPGELPPTLVLAGERDAATPYA
                     GALELRRRLPDAALVTERGAGTHGLAGGPNACVNRHVEAYLIDGKVPARSTDCAARPAP
                     EPEAREVEPQSGGESGSRGARPARPRVAGWPPAAR"
     aSDomain        6753..7874
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B590_RS04725_Abhydrolase_1.1"
                     /evalue="1.10E-21"
                     /label="B590_RS04725_Abhydrolase_1.1"
                     /locus_tag="B590_RS04725"
                     /protein_end="479"
                     /protein_start="105"
                     /score="69.5"
                     /tool="antismash"
                     /translation="ALVFNPGGPGSSGMLFPLVGIFPEWKRLASVYDLVGYAPRGVGRS
                     APLSCRDPREKPDGSWQAPEHPTEKQKAGRRAEAEAYARGCAERGGKRLRHIHSLNNAR
                     DLDVVRAGLGERKLTFMGASYGSYFGALYAQLFPSHVRRMVFDSAVDPSPSRVWYRNNM
                     AQSAAFEKRWADFRRWIASHDAAYGMGTDAAQVLAGYRRAREKLAAEPAGGTVGPAQLQ
                     AVMLRAQYYEDYWPSHAEALAAYLKGDPQPLVNLATPLEEGRVEAENARAVYTAVECND
                     APWPTSWETWDRDHTRLARTAPFETWENAWMNLPCAYWKEPRQTPMDVRVAPGELPPTL
                     VLAGERDAATPYAGALELRRRLPDAALVTERGAG"
     gene            complement(8110..8826)
                     /gene="hemQ"
                     /locus_tag="B590_RS04730"
                     /old_locus_tag="B590_04805"
     CDS             complement(8110..8826)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951588.1"
                     /locus_tag="B590_RS04730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B590_04805"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_023416418.1"
                     /transl_table=11
                     /translation="MSAAPEKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPEDRSGYAD
                     EVQDLFDKLAAKDVTVRGTYDVSGLRADADVMIWWHAETADALQEAYNLFRRTRLGRAL
                     EPVWSNMALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYEWYLLPDEERRAMLADH
                     GKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARRHVREEVPF
                     YTGRRKSVADLVAELA"
     gene            complement(8831..10312)
                     /gene="hemG"
                     /locus_tag="B590_RS04735"
                     /old_locus_tag="B590_04810"
     CDS             complement(8831..10312)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404755.1"
                     /locus_tag="B590_RS04735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B590_04810"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_037969309.1"
                     /sec_met_domain="DAO (E-value: 3.4e-09, bitscore: 35.2,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAETEGNPAGAGGASPRVVVVGGGVAGLTAALRLLDAGAEVTLL
                     EASERLGGKLRAGEIGGVRVDLGAESMLARRPEAVGLAREAGLAGALQPPATASASLWT
                     RDALRPMPKGHVMGVPADPASLAGVLSEEGVARIAEDRTLPRTEVGEDIAVGEYVATRV
                     GREVVDRLVEPLLGGVYAGNAYRISLRAAVPQLFAAAREHTSLTEGVRAIQGRTAADPR
                     TDPVFMGIDGGVGRLPLAVADTVRARGGRIRTGTPVTGLARTPGGWRLTTGTPGTPGAP
                     EALEADAVVLAVPAPQAARLLAAELPAASADLAEIEYASMALMTFAFRAGEAELPQGSG
                     FLVPPVEGRTVKAATFAGRKWRWIAEQEPGLHVVRTSAGRHREEEILGRDDADLLALAR
                     RDLKAATGVDAEPVDAHVTRWDQGLPQYPVGHLARVARVRDQVAKAPGLALCGAAYDGV
                     GIPACVASATEAARQVLAPRREGDPASGSSGHAGE"
ORIGIN
        1 agggggtggc tgccgacggt cagcgcgggg aatccggcgt cggccgccgt ccgcgcggac
       61 cagacgtccc agacggtcgg cagcacgagc ggctgatggt cggcgtgcag ctgctggagg
      121 cgctgagcgc gctcgacggt ggttcgcaag tccatgccgg ggaagctacc ccccggatcg
      181 ccgcgatcca cgtgccgacg gggcgccgtc ctccgcccag ccccgcagct tctccggatt
      241 gcggaccacc cagatgcggg cgacccggcc gtcggcggcg aggtcgaagg cggccacggt
      301 caggacggtc ccggcgcggc gggcgaccag acccggggtg ccgttgaccg gacgttccag
      361 gagttcgagg ccgggggccc tgtccgcgat accggccatg taccgggcga tgtgagcgcc
      421 gcccgccacg gggcgcaggg cggcgccggc caggccgccg ccgtcggcgg tcatcacggc
      481 ggccgggtcg aggaggtcga cgggggccgc gatgtccctg gcctcccacg cctccttgac
      541 gcgccgcacg gtctcggcct gtccgccgcc cggtgcaggg gagccgtccc gtacccgtcg
      601 ccgggcggag gcggcgagct gcttgcaggc ggcgggggtc cggtcgagaa caccggcgat
      661 ctcggcgaag gggtagcgga agacgtcgtg gaggacgaac gcgacgcgtt cggccggggt
      721 catcgcctcc aggacgacga ggaaggccat ggtgacggac tcgtccagca ccagctggtc
      781 ggcggggtcg cagctcggcc cggccggccg gggtggccgg ggagcgggtc gggcagccag
      841 gcgccgacgt agcgctcgcg gcgggcgcgc gccgagccga gcacgtccag gcagacgcgg
      901 ccggtcaccg tggtgagcca ggcgccgggg ctggcgatct cgtcccggcg cgggtccggc
      961 agcgcgtacc agcgggcgta cgcctcctgg acggcgtcct cggcctcggc gagtgaaccg
     1021 agcaaccggt aggccacgtt cagcagttgg cgccgttcac cggtgacctc gccggggtcc
     1081 gccccagccg tgcgaccgct cacccgtccc ccctcgcctg acctttccgg ggcccgtgtc
     1141 gtcgggctgg tgagaccttg atcgacatcg gccgccgccc tcgggcgcgg aagaggggga
     1201 cccggcatga cgacgaccca ggcgacgccc cgcacgaccg tccggacccg cttctcgttg
     1261 ctgcggaccg cactcgccct ccaggccctg acgatcttcg tgcaggcggt ctcggcgggg
     1321 ctgctgctca gcaccgacta cggcgaactc ctgcacggtg tcggggcgcg ggtgatgtac
     1381 ggggcgtcga tgctgtacct gctcgccgcg gtcctcgcct ggaagccggg cggcggctcg
     1441 ccgaaggccg tctggcacgc gtccggcttc ctcgtgctgg cctccgtcca ggtggtgtcg
     1501 gggatcgcgc acctgtcctc gctccatgtg ccgctggggg tgctgatgtt cggggcgagt
     1561 ctggcggcgc tggcccggcg gtgaggaccg ggccagcggc cgtaccccgg accgttcgtg
     1621 gcaggccagg tgggctgtga cggccgtgtc cagggccacg cctggttccg gtggggctgg
     1681 gtgcgtggta cacacgtgcg ggtcacattc ctgtccgcag agccgcagca gggagttgcc
     1741 catgtccgcg acacgccgtc aagtcctcgc ccgtaccggt gccgtcgccg gtatcgcctt
     1801 caccggcagt ctctccgaac tcttcgcggg cacggccgcc gcgaagggga tggggaaggc
     1861 cgggtacggt ccgctggtgc ccgacccgaa gggtctgctg gacctgccga aaggtttccg
     1921 ctaccgggtg ctgtcccggg agggcgaccg gatgctctcc ggcgagggcc aggtgccgag
     1981 caaccacgac gggatggccg ccttcggcgg gcgccggggc caggtccacc tcgtccgcaa
     2041 ccacgagaac cggcccaccg ccccgatccg cgtcccgccc gtccgcgggc tgacgtacga
     2101 cccggagggg atgggcggct gcacggcgct caccctcgac cccgcgcacc gcgtcctcgg
     2161 cgagcgggtc gccatcgccg gtacctcgac caactgcgcc ggcgggccga ccccctgggg
     2221 tacctggctc acctgcgagg agaccgagga caaggccggg accaacggct acaccaagga
     2281 tcacggcttc atcttcgagg tcgacccggt cgacccgcac aggagcggcg cggtgccgct
     2341 gaaggcgatg ggccgcttcc agcacgaggc gatcgcgatc gacccgcgcc acgggatcgt
     2401 ctacgagacc gaggacgcct tcgagcggcc gttcgggctc ttctaccgct tcctgcccga
     2461 gaagccgcgc ggcggcctcg gctcgctgcg ggccgggggc gcgctccagg cgatgcgggt
     2521 gcccggggtg ccggacctct cggcgatcca ggagacgggc gcctcgttcg accgggtgga
     2581 gtgggtggac gtgccggacc cgctcgccga ggagacgccg atccgcttcc aggacttcgg
     2641 cccgaagggc atcacccatg cccagaagct ggagggctgc tactggggcg gccgctcggt
     2701 ctacttcgtc tccagcttcg cccggagcag cgagggctcg gccgccgacc actacgggca
     2761 gatctggcgc tacgacccgg accggcggcg cctcaccctg gtcatcgtct tcggccccga
     2821 caccgacgtg cagctcccgg gcgaggagcc ggacaacatc tgcctcgccc cgagcggcgg
     2881 cctgatggtc gccgaggacg gcgagggcgc ccagcacgtc tacggcgtga ccacgcgggg
     2941 cgaggtctat ccgatggcgc ggggccgcca gaacacgggc acgcccgagg agccggcgtg
     3001 gggcgagttc gcgggcgtct gcttctcccc ggactacgac acgatgttcg tcaactgcta
     3061 cacaccgggg acgaccttcg cggtcaccgg gccctggcac cggtgacgtg agcgggggcg
     3121 gggccgggcc gtcggcctcg ccccccatct cgcgggcccg gccaggcacc cggacggcgg
     3181 aagacggcgg ggcgagacgg gtgaggccac accccccggc cggcattttg cccgatattc
     3241 ggcttttcgc atcctagggt cacggcgtga tcagcatggt gcgcaaggcg aagctccgga
     3301 ccggcacggc gctgggcgcc ctcatggcgg tgggcgcggt ggccgggtgc ggccattcct
     3361 ccccggaccc ccacgacacg gcaccggggc cccgggcctc gcactccgcc tccccggccg
     3421 ccccgccggc gccgcccacg ctggcgcccg gccccggcgg cctcgccccg gtcttcgcca
     3481 acgggccccg gaacgacggc aagaccgtcg cgctcacctt cgacgccgac atgacggccg
     3541 accagggcga gcgcgcggcc ggcggcgagc gcttcgacca cccggagctg atcgcctcgc
     3601 tgcgccgcct gaaggtgccc gccaccgtct tcatgaccgg acggtgggcc gaggagtacc
     3661 cggaccaggc gcgggccatc ggcgccgacc cgctcttcga gatcgccaac cactcctaca
     3721 gccaccacgc cttcaccggt gactgctacg gcctgccgcg ggtcgccgag agcgagatgc
     3781 gggccgacgt ggaacgggcg tacaccgcgt tccgcacggc cggggtgaag aaggcgatgc
     3841 cgtacttccg cttccccggc gggtgttatg acgacacggc gctgcgggag gtctcggcgg
     3901 ccggggtgac ggcggtgcag tgggacgtgg tgagcgggga cgccttcgcc accgacgccg
     3961 gcgcggtggc gcagcaggtg ctggacgggg tgcgccccgg ctcggtcgtg gtcatgcact
     4021 gcacccgcag cgccgccccc gccaccgagc gcgcgctgcg ccggatcgtg cccgccctgc
     4081 gcgagcaggg gtaccgcttc gtgaaggtgt ccgagctgat cggggcggct tcgcggaagg
     4141 gatgaggggg tctcgccgtt tggcagactg gctccgtgac cagcgaaccc ctccccgaca
     4201 gtcccttccg tgacgagcgc accgcccgcg acgaggcacc gcagttcgta ctgccgctcg
     4261 tcgtccggct ggagcgcgcc gagccgcccg ccctcaccga cgcgctccag acggccgccc
     4321 gggccgtcct gacgctgctc agcgatcccc gctcggaggg ggacggcgtc tgggcggagg
     4381 cggtgcggga ctggcaggac gcgcgtatcc gcaaggtggt gcggcgggcc cggggcgcgg
     4441 agtggcgcaa ggccgcggcc ctgccgggga tcacggtcga ggacggcggc gccgaggtgc
     4501 gggtctaccc gccggtgccg ctggacggct ggcccaggga gctggccaag ctccaggtct
     4561 ccggcaccga gctggaggac cccgtggagc cgggcgagcc cgcggccggc gtcccggtgc
     4621 tctggctcaa cccggacgtg cccatgtcgg cggggaaggc gatggcgcag gcgggccacg
     4681 gcgcgcaact ggcctggtgg cagctgtcgg gggcggaacg caacgcctgg cgcgagcagg
     4741 ggttcccgct ggcggtgcgg accgccggcg cggagcgctg gaaggagctg gtggagagcg
     4801 ggctgccggt ggtgcgggac gccgggttca cggagatcgc gcccgggtcg tgcacggtgg
     4861 tggcggagca tcccgcattg gtggggtgac ggcggcgaag ctcagatcct gctctgaaga
     4921 gggcggatga cggttccggg acggacggcg gcgggcaggg tggccgcagg gaccgggggg
     4981 tccctggagg gggtggcggt atggaacggc tcggaacggg gatcggctgg cggcccgcga
     5041 tcgcggaggc cgtcgaggcg atggcgggga tcgagtgggt ggaggtggtg gcggagaacg
     5101 tctgccccgg tcaccttccc gactccctgc ggcggctgcg ggagcgtggg gtggccgtgg
     5161 tgccgcacgg ggtggggctg ggactgggtg gtgcggagcg gcccgatccg gtgcggctgg
     5221 cggcgctggc cgagcgggcc gaggcgctgg actcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtcagggc gggcggggag cggaccgcgt cgcgcgggct ggaggccggg cacctgctgc
     5341 cggtcccccg gacccgggaa gggctcgcgg tgctctgcga gaacgtgcgg atcgcccagg
     5401 accagctgcc cgtgccgctc gccgtggaga acatcgcggc gctcttctcc tggccgggcg
     5461 aggagctgac ggaggggcag ttcctctacg agctggccga ccggaccggg gtgcggctgc
     5521 tgatcgacgt cgccaacctg cacaccaacc acgtcaacca gggcgaggac cccgccgccg
     5581 ccctcgacgc gctgccgctg gaggcgctgg cgtacgtcca tgtggcgggc gggttcgagc
     5641 gggacggggt gtggcacgac agccacgccc acccggtgcc ggatccggtg ctggcggtcc
     5701 tggccgagct ggcctcgcga gtgcggccgc cgggggtgct gctggagcgg gacgagcggt
     5761 tcccgtcggc ggccgggctg gaggcggagg tcgcgaagat ccggcggacg gtggcggcgg
     5821 ccggccggga ccgggtttcg gtgggagcgg gggcgcggcc cgggtccgta ccggcgtcgg
     5881 gcagcccggt ggcggacgag gcggctcgga cgcggctggc cctcgggcag acggcgctgc
     5941 tttcggcgct ggtcgcgggg actccggcac cggaggggtt cgaccggggc cggctgcggg
     6001 tgcagaaccg gatgctgacg gccaagcggg gttccgtcgt ggcctccctc gccccggagc
     6061 tggagcggat cctgggcgac acgtaccggc cgcgcttcct gcggtacgcg gcgggccggc
     6121 cgctgaccgg cgggtaccgc gcggacgcgc tcgccttcgc gctccacacc ttgcgggagg
     6181 aaccgccggc cgagcgggcg gtccggcgcg cgctgcgcga ctggtggatc gagcgctcgg
     6241 gccgcgcgtc cctggtcggc aggccctggg cccggtgggc cagggtggcg cgcttcgtgc
     6301 tgctggggcg ctgagaccgg cggccgtgtt gggccgatcc gagcaggtct gtccaacacg
     6361 cgccccggtg cttgcccttg ccgatcggcc tgcgaatcat ccctagggcc tgtcatcggc
     6421 cggacgaaag ggacgcgatg cgagcgccgc tcatcctcac cgccgccgga gccctgctcc
     6481 tcaccccgct cgcggctgcc ccgcaggctc ccgcggccgg tgctccggcc ccgtccggcg
     6541 gccccgaggt gcgcggggtg gcgctcgcgg ccgagcgggc ggcgcggacc ccgatcgcct
     6601 tcgggccgtg cccggacgag gaggggctgc cccggtcgct ggagtgcggc acggtacggg
     6661 tgccgctgga ctacgcgcgg ccggacggcg agcagatcga cctgaccgtg agccgggcca
     6721 aggcggcggg cccgaagggc gagcggcagg gcgcgctggt cttcaacccg ggtgggcccg
     6781 gctcctcggg gatgctcttc ccgctggtcg ggatcttccc cgagtggaag cggctggcct
     6841 cggtctacga cctggtcggg tacgcgccgc gcggggtcgg ccggtcggcg ccgctctcct
     6901 gccgggaccc gcgcgagaag ccggacggga gctggcaggc ccccgagcac ccgaccgaga
     6961 agcagaaggc cgggcgcagg gccgaggccg aggcgtacgc gcgggggtgc gccgagcgcg
     7021 gcgggaagcg gctgcggcac atccactcgc tgaacaacgc ccgcgacctg gacgtggtgc
     7081 gggccggcct cggtgagcgc aagctgacgt tcatgggcgc gtcgtacggc tcgtacttcg
     7141 gggcgctgta cgcgcagctc ttcccctcgc acgtgcgcag gatggtgttc gactcggcgg
     7201 tcgacccctc gccctcgcgc gtctggtacc ggaacaacat ggcgcagtcg gcggcgttcg
     7261 agaagcggtg ggcggacttc cggcggtgga tcgcctcgca cgacgcggcg tacgggatgg
     7321 gtaccgacgc ggcccaggtg ctggcggggt accggcgggc gcgcgagaag ctggcggccg
     7381 agccggccgg gggcacggtc ggcccggcgc agctccaggc ggtgatgttg cgggcccagt
     7441 actacgagga ctactggccc tcgcacgcgg aggcgctcgc ggcgtacctg aagggtgatc
     7501 cgcagccgct ggtcaatctg gcgaccccgc tggaggaggg ccgggtggag gcggagaacg
     7561 cgcgggccgt ctacacggcg gtggagtgca acgacgcgcc gtggcccacc agttgggaga
     7621 cctgggaccg cgaccacacc cgtctcgccc gcacggcgcc gttcgagacc tgggagaacg
     7681 cctggatgaa cctgccgtgc gcgtactgga aggagccccg gcagacgccg atggacgtgc
     7741 gggtggcccc cggtgagctg ccgccgacgc tggtcctcgc cggggaacgg gacgcggcga
     7801 ccccgtacgc gggcgcgctg gagctgcggc ggcgcctgcc ggacgccgcg ctggtgaccg
     7861 agcggggcgc gggcacgcac gggctggcgg gcgggccgaa cgcctgcgtc aaccggcacg
     7921 tggaggcgta cctgatcgac gggaaggtcc ccgcgcgctc caccgactgc gcggcccggc
     7981 cggcccccga gccggaggcg cgggaggtcg agccgcagtc gggaggggag tccggcagcc
     8041 ggggtgcgcg gccggcgcgc ccccgggtcg ccgggtggcc cccggcggcc cggtgaggcg
     8101 gagcggggct caggcgagct cggcgaccag gtcggcgacg gacttgcggc gcccggtgta
     8161 gaagggcacc tcctcgcgga cgtggcgacg ggcctcggag gcgcgcaggt ggcgcatgag
     8221 gtcgacgatg cggtacagct cgtcggcctc gaaggcgaga atccactcgt agtcgccgag
     8281 ggagaacgag gcgaccgtgt tggcgcggac gtccgggtag ccgcgcgcca tcttgccgtg
     8341 gtcggcgagc atcgcccggc gctcctcgtc gggaaggagg taccactcgt aggagcggac
     8401 gaacgggtag acgctgatgt agtcgcgggg ggtctcgtcg gcgaggaacg ccgggatgtg
     8461 cgacttgttg aactcggcgg gccggtgcag cgccatgttc gaccagaccg gctccagagc
     8521 gcgcccgagg cgggtgcggc ggaagaggtt gtacgcctcc tggagggcgt cggcggtctc
     8581 ggcgtgccac cagatcatca cgtcggcgtc ggcgcgcagc ccggagacgt cgtaggtgcc
     8641 gcggacggtg acgtccttgg cggcgagctt gtcgaacagg tcctggacct cgtcggcgta
     8701 gccggaccgg tcctcgggga gcacgtcgcg gagcttgaag acggaccaca gggtgtagcg
     8761 gatgacctcg ttgaggtcct tggccttctt gcccgcgttg gggatcttct cgggagcagc
     8821 actcatgggg tcattctccc gcgtgcccgg acgagcccga cgccgggtcg ccttcccggc
     8881 ggggcgcgag gacctggcgc gccgcttcgg tggcggaggc gacgcaggcg gggatgccga
     8941 cgccgtcgta ggcggcgccg cacagggcga ggccgggggc cttggcgacc tggtcgcgga
     9001 cgcgggcgac gcgggcgagg tggccgaccg gatactgggg caggccctgg tcccagcggg
     9061 tgacgtgggc gtcgaccggc tcggcgtcga ccccggtggc ggccttgagg tcgcgacggg
     9121 cgagggcgag gaggtcggcg tcgtcacggc cgaggatctc ctcctcgcgg tggcggccgg
     9181 ccgaggtgcg gacgacgtgc agccccggct cctgctcggc gatccaccgc cacttgcggc
     9241 ccgcgaaggt ggcggccttg acggtacgtc cctcgaccgg cggcacgagg aagccgctgc
     9301 cctgcggcag ctccgcctcg cccgcgcgga aggcgaaggt catcagggcc atcgaggcgt
     9361 actcgatctc ggcgaggtcg gcggaggcgg ccgggagttc ggcggcgagc agccgggccg
     9421 cctggggtgc gggtacggcg aggaccacgg cgtcggcctc cagggcctcg ggcgcgccgg
     9481 gggtgccggg ggtgccggtg gtcagccgcc agccgccggg ggtgcgggcg aggccggtga
     9541 cgggcgtgcc ggtgcggatg cggccgccgc gggcgcggac ggtgtcggcg acggcgagcg
     9601 ggaggcggcc gaccccgccg tcgatgccca tgaagaccgg gtcggtgcgc gggtcggcgg
     9661 cggtgcggcc ctggatcgcg cggacgccct cggtcaggga ggtgtgctcg cgcgcggcgg
     9721 cgaagagctg ggggacggcg gcgcgcagcg agatgcggta cgcgttgccg gcgtagacgc
     9781 cgccgaggag gggttcgacg agccggtcga cgacctcacg gccgacccgg gtggcgacgt
     9841 actcgccgac ggcgatgtcc tcgccgacct cggtgcgggg cagggtgcgg tcctcggcga
     9901 tccgcgcgac gccctcctcc gagaggaccc ctgcgaggct ggccgggtcg gcggggacgc
     9961 ccatgacgtg gcccttgggc atggggcgca gggcgtcgcg ggtccacagg gaggcgctgg
    10021 cggtggcggg gggctggagg gcgccggcca gtcccgcctc acgggcgagg ccgaccgcct
    10081 cggggcggcg ggccagcatc gactcggccc ccaggtcgac gcggacgccg ccgatctcgc
    10141 cggcgcggag cttgccgccg agccgctcgg aggcttccag gagggtcacc tcggccccgg
    10201 cgtcgagcag gcgcagcgcg gccgtgagcc ccgcgacgcc gcctccgacg accaccacac
    10261 gggggctcgc gccacccgct cccgccgggt ttccctctgt ttccgcgcgc atggagccac
    10321 tgtctcaaac cggacgcggc gggcggctgc cggggcggcg cggttccgtt attcgttcgc
    10381 gacccgggag gcgaaaccca ccggtgcgcc gggccgtcgt agcggcacag gcgccccggc
    10441 cggggcgccc gaaggacacg agggggcggc ggatgagccg gatgcgggga acgagcggcg
    10501 gccggatcgt ggggcggcag gcagcggtgg tgctggcggc ggcggtactg ggtctcgcgg
    10561 ggtgttcggc cgggtcggac tccggggcgt cgccggcggg ggaccgtgcg gccgcgccgt
    10621 cggcggccga cggcgcgggc ggcggtcagg ccgacgacgc gaagcgggag tcggcggacg
    10681 aggccgcgga cggaagggcg cgccccgagc tgaagggcgt ccacctgatc cgtacggccg
    10741 agctgcatct gcgggtgaag gacgtgccct cggcgctggc ctcggcccgg tcggtggtcg
    10801 agggcgcggg cggctacgtc ggcgacgagt cgacccggcg cgactcccag ggccacgagc
    10861 ggtcgaccgt ggtggtccgc gtcccgcagg agcggtacga ggcggtgctg gactcgctcg
    10921 gcgaggacgg cgaggtggtc tcccggcgga ccagcgcgga ggacgtcacc gaggaggtgg
    10981 tggacgtcga gagccggctg aagtcgcagc gggccagcgt ggcgcgggtc cgggagctga
    11041 tggaccgggc ggagaagctg agcgacatcg tctcgctgga gggcgagctg agcagccgcc
    11101 agatggagct ggaggcgttg ctggcccagc agtcctcgct gaaggaccgc acctcgatgg
    11161 cgacggtcac cctccagctg accgagcgga cggcgaagac ggtgtcgaag gaggacgacg
    11221 gcccgacctt caccgacgcg ctctccggcg gctggtcggc gttcgtgggg acgctgcgct
    11281 ggctgaccgt ggtggtcggg gtggcgctgc cttt
//
