LOCUS       NZ_CP106651            20582 bp    DNA     linear   CON 16-MAY-2024
DEFINITION  Streptomyces sp. GBA 94-10 4N24 strain GBA 94-10 plasmid pGBA1,
            complete sequence.
ACCESSION   NZ_CP106651
VERSION     NZ_CP106651.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. GBA 94-10 4N24
  ORGANISM  Streptomyces sp. GBA 94-10 4N24
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 198437)
  AUTHORS   Ian,E., Malko,D.B., Sekurova,O.N., Bredholt,H., Ruckert,C.,
            Borisova,M.E., Albersmeier,A., Kalinowski,J., Gelfand,M.S. and
            Zotchev,S.B.
  TITLE     Genomics of sponge-associated Streptomyces spp. closely related to
            Streptomyces albus J1074: insights into marine adaptation and
            secondary metabolite biosynthesis potential
  JOURNAL   PLoS One 9 (5), e96719 (2014)
   PUBMED   24819608
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 198437)
  AUTHORS   Ruckert,C., Busche,T. and Kalinowski,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2022) CeBiTec, Uni Bielefeld, Sequenz 1, Bielefeld
            33615, Germany
REFERENCE   3  (bases 1 to 198437)
  AUTHORS   Ian,E., Sekurova,O.N., Malko,D.B., Ruckert,C., Borisova,M.E.,
            Albersmeier,A., Kalinowski,J., Gelfand,M.S. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2013) CeBiTec, Bielefeld University,
            Universitaetsstr. 27, Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-NOV-2021
            Assembly Method                   :: flye v. 2.9-b1768
            Assembly Name                     :: GB94_v2
            Genome Coverage                   :: 229.4x
            Sequencing Technology :: Illumina MiSeq;Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000495635.2-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 06:31:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,298
            CDSs (total)                      :: 6,204
            Genes (coding)                    :: 5,988
            CDSs (with protein)               :: 5,988
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 100 of 216
            Pseudo Genes (incomplete)         :: 164 of 216
            Pseudo Genes (internal stop)      :: 24 of 216
            Pseudo Genes (multiple problems)  :: 67 of 216
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:13:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 10608
            Orig. end                         :: 31190
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP106651.1.
            Bacteria and source DNA available from Wittmann Lab, Saarland
            University, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20582
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="linaridin"
                     /region_number="1"
                     /rules="(cypemycin or cypI)"
                     /tool="antismash"
     cand_cluster    1..20582
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cypemycin or cypI)"
                     /kind="single"
                     /product="linaridin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20582
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10582](+)"
                     /cutoff="20000"
                     /detection_rule="(cypemycin or cypI)"
                     /neighbourhood="10000"
                     /product="linaridin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10582
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cypemycin or cypI)"
                     /neighbourhood="10000"
                     /product="linaridin"
                     /protocluster_number="1"
     gene            773..1381
                     /locus_tag="B591_RS30580"
                     /old_locus_tag="B591_30343"
     CDS             773..1381
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018846231.1"
                     /locus_tag="B591_RS30580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_30343"
                     /product="response regulator transcription factor"
                     /protein_id="WP_023423353.1"
                     /transl_table=11
                     /translation="MSIRVLLADDEKLILGALSALLATEDDLEVVGETAGGVQAVEMAK
                     NLLPDVCVLDLSMPDRGGISVVRDLQVAQPSIPCVIVTSHTAPGYLASVMAAGARGFVP
                     KTTPARELAAIIRTVHRGARYIDPVVAADAIAAGDSPLTRREADILALSADGAPIDMVA
                     QRAALSSGTVRNYLSKIAGKLKSANRHEAAYVARRQGWI"
     gene            complement(1801..>2911)
                     /locus_tag="B591_RS30585"
                     /pseudo=""
     CDS             complement(1801..>2911)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006141892.1"
                     /locus_tag="B591_RS30585"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="IS110 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="RVFDKQLPNIAPALRAVFGKLTAKFGTPW"
     misc_feature    complement(2952..2966)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.07"
                     /tool="antismash"
     gene            3461..4903
                     /locus_tag="B591_RS30590"
                     /old_locus_tag="B591_30358"
     CDS             3461..4903
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452027.1"
                     /locus_tag="B591_RS30590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_30358"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_023423354.1"
                     /transl_table=11
                     /translation="MTDAAIVGTGPNGLAAAVTLLRAGLSVELYEAHDTIGGGLRTESL
                     FDSDVVHDICSAVHPMAAASRFFREFDLPARGVDLLQPEIPYAHPLLGEDTVLAHRDLA
                     ETSAHLGADGGRWRRLMAPLAAHSQGVVDLILSGQRSVPDDLSAPLLLAPRVLAHATRM
                     PFRTERARALLTGVAAHAVGRLPSLAASAVSLLLGHLAHTSGWPLPRGGSARIAQALAA
                     DIAAHGGRVHTGQLIKDLGELEGVPLVLLDTSPKGLLQLAGDRLPSPYRRALERFRYGP
                     GASKVDFLVSEPIPWTDPQTGRAGTVHLGGTRADMFRTESAAVRGIQDPEPFVLLVDPA
                     ATDPGRAVGGKRPVWAYAHVPNGDPRDPVDLVRRRIEQYAPGFGDTVIAARGLSAAQYE
                     TYNPNYVGGDIGSGAMTLRQSLLRPTAKWDPYKVSLPGLYLCSAATPPGPSVHGMSGHL
                     AALSALRREHGIRVPPPLGPHA"
     gene            complement(5224..5979)
                     /locus_tag="B591_RS30595"
                     /old_locus_tag="B591_30363"
     CDS             complement(5224..5979)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008749662.1"
                     /locus_tag="B591_RS30595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_30363"
                     /product="IS5/IS1182 family transposase"
                     /protein_id="WP_023423355.1"
                     /transl_table=11
                     /translation="MLVYPSSIDLSSRTLRFLTGRLVARRGEIGTRWRRLTAGRQALLA
                     LAHLRCGDTYTQLAAGFGIGIATAYRYIREAVDVLAALAPTLAEAMRIARTKAFVILDG
                     TLLPIDRIAADTPYYSGKHKRHGMNVHVLTDPFGRLLWASPALPGSTHDLTATRTHGIV
                     EALADADLKCWADKAYQGAGGSIRVPFRGRRLKRWQRRHNSTHAKIRCLGEQAMATLKT
                     WRLLRKLRCSTNRITAIVQAVLVLHHASA"
     misc_feature    6343..6359
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.61"
                     /tool="antismash"
     gene            <6377..6562
                     /locus_tag="B591_RS30600"
                     /pseudo=""
     CDS             <6377..6562
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014043973.1"
                     /locus_tag="B591_RS30600"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="transposase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="PPNGQLTPTERTVNRALATPRAPVERGMARLKSWRIFRRSRCSPN
                     PMTSIAKAVFTLKRQR"
     misc_feature    complement(7195..7210)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.38"
                     /tool="antismash"
     misc_feature    complement(7234..7247)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 19.15"
                     /tool="antismash"
     misc_feature    7234..7250
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 21.26"
                     /tool="antismash"
     misc_feature    7238..7252
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.63"
                     /tool="antismash"
     misc_feature    complement(7430..7444)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 18.39"
                     /tool="antismash"
     misc_feature    7502..7515
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.57"
                     /tool="antismash"
     gene            7531..7722
                     /locus_tag="B591_RS30605"
     CDS             7531..7722
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018824030.1"
                     /locus_tag="B591_RS30605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_037788156.1"
                     /transl_table=11
                     /translation="MGKIAAFMNQQELGSLEDGLTKTDGTPVMATPAVAVPVTVKITAG
                     IVAFTIPVGAYVAGRVVN"
     gene            7765..7965
                     /locus_tag="B591_RS30610"
                     /old_locus_tag="B591_30368"
     CDS             7765..7965
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B591_RS30610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B591_30368"
                     /product="hypothetical protein"
                     /protein_id="WP_023423356.1"
                     /transl_table=11
                     /translation="MTHIIESVRSITEPTLTEGQKDRLTRTMPVAFTPAVLVTTPYAGG
                     GLAAGAAVVGAYELGRAAGGK"
     gene            8199..10004
                     /locus_tag="B591_RS30615"
                     /old_locus_tag="B591_30373"
     CDS             8199..10004
                     /NRPS_PKS="Domain: Abhydrolase_1 (324-445). E-value:
                     3.5e-11. Score: 35.1. Matches aSDomain:
                     nrpspksdomains_B591_RS30615_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006132581.1"
                     /locus_tag="B591_RS30615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_30373"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_234025470.1"
                     /sec_met_domain="PF00561 (E-value: 6.1e-10, bitscore: 32.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 4.5e-17, bitscore:
                     56.9, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSERLSALTTLSSSLEYLHQHKQMDPGGLNDWEIMKEAPETKNLA
                     IRRLTEAVSGKKVTVAIHTARAAVSLGLLFSGSSRWRGAGSLFLTASTSLLQTRHRYGT
                     DGSDQVANLVQTATGLARLSKSPQTKDALLWYVALQASLSYAASGWVKLLGDKWRDGSA
                     LPGVMRTRTYGFESAYHLTQRYPKTAKYLQYGVLAMECLFPVVYLAGGKLARPMIAAAG
                     SFHVANGFVMGLGRFMTAFPAMHPMVAYTTAPKSHPSVAGRDDRAVKAAALLLAGGVVA
                     AAAVAAQRRAVVLEGWPTSRKCVTRHGNELMYETGGRDDETDAPMLVFCAGLTSTSEHF
                     AWITERFVNDSDSAVVSYARAGYAGSLRRTNAPFVLEESVDDLEDLIRATVPGHRKAVL
                     VGHSLGGELIRRVSARIPDRVAGLIYLDPSHPAELLRSERQSEGAKDLNDNITHAARYL
                     SMGTGILMSRPGWLDRLPANYRDKVFAQYTDARLWAAGKREWAAVEKDFRSFDGDLPQV
                     AAPALIIAAQQTVDQDPEQLLMYNELARAHQRGDVKVVEGADHDSLLTNALFAHKTVQH
                     MTAFMKDSTGGEHSESVIDEQGAPK"
     aSDomain        9171..9533
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B591_RS30615_Abhydrolase_1.1"
                     /evalue="3.50E-11"
                     /label="B591_RS30615_Abhydrolase_1.1"
                     /locus_tag="B591_RS30615"
                     /protein_end="445"
                     /protein_start="324"
                     /score="35.1"
                     /tool="antismash"
                     /translation="PMLVFCAGLTSTSEHFAWITERFVNDSDSAVVSYARAGYAGSLRR
                     TNAPFVLEESVDDLEDLIRATVPGHRKAVLVGHSLGGELIRRVSARIPDRVAGLIYLDP
                     SHPAELLRSERQSEGAK"
     gene            10001..10582
                     /locus_tag="B591_RS30620"
                     /old_locus_tag="B591_30378"
     CDS             10001..10582
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     linaridin: cypI"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452032.1"
                     /locus_tag="B591_RS30620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_30378"
                     /product="hypothetical protein"
                     /protein_id="WP_023423358.1"
                     /sec_met_domain="cypI (E-value: 5e-35, bitscore: 114.3,
                     seeds: 22, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNPAALVKDAFTGPGSLARVVGAGMLVATVAAQHPHPAFDRVRSR
                     DLFSVVPNWKFFAPNPATHDFHYVYRTLDHTGGTSEWSEIDMIEPRKMHQAFWFATRRP
                     EKSVFDLCTAILQQATKTGATEVQKTSSYRVLAEFIRKTIRDQQGDENVKGFQFAVVRA
                     AGHDTNEEPEPLFISPYTPMRRFGPHLAAA"
     gene            10634..11452
                     /locus_tag="B591_RS30625"
                     /old_locus_tag="B591_30383"
     CDS             10634..11452
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B591_RS30625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B591_30383"
                     /product="hypothetical protein"
                     /protein_id="WP_023423359.1"
                     /transl_table=11
                     /translation="MTTPPIPADDFVGYLRTYTADAFASEQSVEDVWVRYHVPNATHVV
                     NRKQRDRAGVLKNLSQWRADQTPYELWIHDVVVDGSRAAARYTISKPLVMQVRHNTETV
                     MFADLADDGRVASTVTTARSTYGWGSSDGTWDSGAGSVGVPKGAPTDPAAYLTTFGELA
                     KDPDASLEEAYDRFHTPDSVQFINGTSLQRPAALQALAAARDKGPDYTLDIHAALSQGN
                     RFAVRYSMVPSRHGRPASEMEVFTFGELADDGRVRLLRSAMQTRSGYWPT"
     gene            11508..12260
                     /locus_tag="B591_RS30630"
                     /old_locus_tag="B591_30388"
     CDS             11508..12260
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018824034.1"
                     /locus_tag="B591_RS30630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_30388"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_023423360.1"
                     /transl_table=11
                     /translation="MTSTVADIGYGTQFATWYDRIFPKGGDAQATADVLAGLHPDPGAG
                     TLEFGVGTGRIALPLSQKTGQVMGVDSSPEMLAQLAEAGSENVVPVHADIRTYTDTRQY
                     GLVYVVCGTLSMLLSAEDQQEAVCRAAELLTPGGRLVVETGNLPAVQALHDGQARCTLF
                     TPYPEPGTGLQTHSTLSSEIWQCSHIWYESDGSTRVGSELARLTTPDEVDRYAAVAGLA
                     PEGRYADWSLSSYTEQYPMFLTTYTKNA"
     gene            12303..12479
                     /locus_tag="B591_RS30635"
     CDS             12303..12479
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B591_RS30635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157875472.1"
                     /transl_table=11
                     /translation="MTADRAVWFAVAFVFAAPSMSAFFREDGRFTRRAVALGVVFATSV
                     AAIVAIAFGQAPS"
     gene            12476..14209
                     /locus_tag="B591_RS30640"
                     /old_locus_tag="B591_30393"
     CDS             12476..14209
                     /EC_number="7.-.-.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018824035.1"
                     /locus_tag="B591_RS30640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_30393"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_023423361.1"
                     /transl_table=11
                     /translation="MNFSAIVRRHRVLLIVGILLGLGGTAATLVQPLLIGDLIEAVALD
                     QSVTWPILSIAALFVADAVLGATHFYLIGRAGEDIVLDMRSTLTGRLLHSRIRAFHKLE
                     HGDVFTRTVSDTSLARVALSSSVAQMITAGFTTVGCIAVMAWIDWQLLLVTAGCLGVAS
                     ALALILARAVRKAAVQNREDTSDYGSGLLRVLGALTTVKASGAERREAERIETLAKAAR
                     GSGIRVTRLSAMLMPAMNVGTQVSLAVVIAWGMARTATGALSAADLTSFIMYLFYLVSP
                     LVMFFMSLGELQQGRAAIDRVNDLATVDQETAEISAGLVATPNLDESDDFPAVEFRNVT
                     FSYSDTASPTLDDVSFTLPATGITAIVGPSGAGKSTLFHLIERFYDPDKGGIRLFGEDI
                     SLLPLRELRSRIGLVEQGSPLMRGTVRENLVYTRPDATAEEIAQAVEAAHLTDVIATLP
                     DGLDTALGEGGTGLSGGQRQRLAIARALLTRPAVLLLDEATSNLDSDSETALRSALTTI
                     GATCQVLTIAHRVSTTVGADHILVIEEGRLRARGSHPELMAGDPTYRRLASQQLAPLGS
                     A"
     gene            14292..15143
                     /locus_tag="B591_RS30645"
                     /old_locus_tag="B591_30398"
     CDS             14292..15143
                     /EC_number="3.4.24.-"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057909.1"
                     /locus_tag="B591_RS30645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_30398"
                     /product="M23 family metallopeptidase"
                     /protein_id="WP_023423362.1"
                     /transl_table=11
                     /translation="MPVILWKRYGLVFAAGALCLVVNAQGIGPLWYTGVALLIIGQVMR
                     VTLIRAQRPPLDREPVLLRLPVTGRWLALNGPATKVPSHTHSHAQTYAIDLKYVPEGRG
                     ERSAPQAPPFRWLWPLARSPRLYPTFGMPLLAPADGVVVATAGRQRDHLSRMSFPAFLF
                     LYLEGFVRSLGWPCHLMGNHIILDLGDGAYAAFAHLRRGSLRVAPGDRVTAGQEMAECG
                     NSGNSSEPHLHFQLMDGPDVMTARGVPFAWSYRDDDGVEHTGVPEDTTHCVPVAPLPER
                     SA"
     misc_feature    complement(14326..14341)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.44"
                     /tool="antismash"
     gene            15279..15578
                     /locus_tag="B591_RS30650"
                     /old_locus_tag="B591_30403"
     CDS             15279..15578
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1164: transposase
                     IS3/IS911 family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /locus_tag="B591_RS30650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_30403"
                     /product="transposase"
                     /protein_id="WP_078539200.1"
                     /transl_table=11
                     /translation="MKNYPPQFKADAVALYESRPEATIRSVAADLGINPETLRDWVRAA
                     GVSRPRGRWTQEPALPSVPLEAENAALRKKVRELEEEREILRKAAKYFAGETRW"
     gene            15846..16616
                     /locus_tag="B591_RS30655"
                     /old_locus_tag="B591_30413"
     CDS             15846..16616
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007268373.1"
                     /locus_tag="B591_RS30655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_30413"
                     /product="IS5-like element IS112 family transposase"
                     /protein_id="WP_037788157.1"
                     /transl_table=11
                     /translation="MAGVITASEPSWIAPFSGLSTRQFGKLVTVLRREGADAVRKGRPW
                     SLPLEDRALLVAAYWRTNLTMRQLAPLFGVSKSAADRIIDHLGPMLALQPRKRFAKDTV
                     LIVDGTLVPTRDHTIAERSKNYRYSTNHQVVIDADTRLVVVVGRPLAGNRNDCKAWEES
                     GAKAAVGKTLTIADGGYPGTGLVIPHRRERGQAGLPDWKEEHNKSHKQVRARVEHVFAR
                     MKTWKILRDCRLKGDGVHHAMLGIARMHNLALTG"
     gene            complement(16903..17622)
                     /locus_tag="B591_RS30660"
     CDS             complement(16903..17622)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B591_RS30660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_158698166.1"
                     /transl_table=11
                     /translation="MTQSFHGIESLPNPERFIHFSGRDADTTCKFGDQTWVPSAPLERR
                     GIYGVELTLTVDDPPPLRTHLPATGLPSGSASQAHRPATLGLPSQSDGRRPSLHEERAA
                     QGTTSTNQSTHKIGTYAQKPQPLVSAQPSTAAVETTGAHTENTAVPDPPTLEHEPSGSG
                     LPAELLALQRSADLEREKLYALHSEGERTCQRRAWFEAAATAQAAITHYARTAQLNRFG
                     LEQEVRRAVRSDDDITM"
     gene            17621..18873
                     /locus_tag="B591_RS30665"
                     /old_locus_tag="B591_30423"
     CDS             join(17621..17900,17900..18873)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1164: transposase
                     IS3/IS911 family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611777.1"
                     /locus_tag="B591_RS30665"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="B591_30423"
                     /product="IS3 family transposase"
                     /protein_id="WP_234025469.1"
                     /ribosomal_slippage=""
                     /transl_table=11
                     /translation="MARPSSYPLELRKRAVRMVAEVRGDYPNESAALRAVAQKLGIGSA
                     ETLRNWVKRDEIDSGQRPGTTTEESAQIKAMKKEIAELKRANDILKAAGKFLRGRARPA
                     THTLVAFIDEHRARFGGVEPICRVLTEHDCKIAPSTYYAHHKRLQVPSARTIRDAELKP
                     LIQQIFDANYRVYGARKVWRELHRQGHFVARCTVERLMRELGVAGAVRGKKIITTIPDG
                     SVERAPDLLDRNFVAPAPNRCWVADFTHVSTWSGVVYVAFVVDTFSRRIVGWSAALSKE
                     TRLVLDALDMALWQRDRDEKPHQRGELIHHSDAGSQYTSFRLAEHLDAAGIAASIGSVG
                     DAYDNALMESTIGLFKTELIKPGQPWRTLSQVELATAEWIDWYCHRRLHGEIGHIPPVE
                     YETNYYLATTKPQVTTKI"
     gene            complement(18857..19900)
                     /locus_tag="B591_RS30670"
                     /old_locus_tag="B591_30428"
     CDS             complement(18857..19900)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="B591_RS30670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="B591_30428"
                     /product="hypothetical protein"
                     /protein_id="WP_037788158.1"
                     /transl_table=11
                     /translation="MANGVWHSGLQRTLELDKEDLGLGGAWPHLSELIESLLQPVAERD
                     RELLVCVESSQGQRCKAELSGVKSPHMYVRKHRGPDGVLRLRAAHLPTAHQMTSEESDR
                     HKAMKDFLARTGESAGLEVQVEATARNRTSRPDVTINGPDGVSLGCEAQYYNASAGTVL
                     RRSKAHADSGLIANWITHDDRFHLIDRSNWMLTREMSWKEISSAVDISLMGGFRVLVEW
                     RCTAGAERPCPDARVKTGCGKMHLHWDSPRRLDDEGTGWTSHKGNTRGVTVGQTLIGAA
                     TGSVTPLFAASRKDRRSGAYMWVPADDKTRWTEYRADEAAPPEEQQAPEEHILNRPGFG
                     RDLRFWL"
ORIGIN
        1 ccggagcaac gtgcgtcgtg gtcgccgccg ccgctagccc gcacgccccc ctgccttcgg
       61 cgctgagcgc cggaggcgtc cttctgacgg tcctcctcgc cgtccgcagc gcgatgcggc
      121 ccctctccgt catctcccgg ctgagtggtg ccgcggacgc agccgccctg ctaggggtga
      181 ccgaagaaag gctgcggatc tctcgcgact tgcacgacac gctgggacgg cacctggcca
      241 tcatcgcgat taagtccgaa ctcatcgtcc gcaccagaag gctggaggag atggaggagg
      301 tgcaggtgct ggcaagggcg tcgcagggcg atctgcgggc ggtggtgaac gccgcgcgca
      361 ccgcgtcgct cgacgaggaa cttgaaggtg cctgctccct gctccgagcc tacggcgtcc
      421 ggtgcgacgt gaccggcggt cacagcggcc ttcgcctgcc ttgcgaggtg cgagcggctc
      481 tcggctgggc cgttcgggaa gcggccacca acatcattcg gcacgcgtac gacgccactg
      541 catgcacgat cacgctcagc ctgtctggcg cgggcgaagc gagagagtgt gtcctgaggg
      601 ttgtgaacga cggcatgcgt gacaggccgg accggcgcct gaatctcggc gtgggcatga
      661 ccgggctgag gcagcgcgtc gcaccgctgg gagggcgcct ggagcacggc ctccgcgcca
      721 acgggaccta ccgcctcttg gtcgctgtcc ccttgcgcga gaggtaacga gcgtgagcat
      781 tcgagtcctg ctggccgatg acgagaagct aatcctgggg gcgctctccg cgctactggc
      841 gacagaggac gacctcgaag tggtcggtga gaccgccggc ggcgtgcaag cggtcgagat
      901 ggcgaagaat ctcctcccgg acgtctgcgt actcgatctg agcatgcccg acaggggcgg
      961 aatcagcgtc gtccgcgacc tgcaggttgc tcagccctcc ataccgtgcg tcatcgtcac
     1021 ctcccacacg gcgcccgggt atctcgcctc ggtcatggcg gctggagcta gggggtttgt
     1081 acccaagacg acgccggcga gagagctggc cgccatcatc agaacggtcc accgcggcgc
     1141 ccgctacatc gatccggtag tggccgcgga cgcaatcgcg gcgggagact cccctctcac
     1201 gcggcgggag gcggacatcc tcgcgctgtc cgccgatggg gcccctatag acatggtggc
     1261 gcagcgagcg gccctgtcct ccgggaccgt acgcaactat ctttcgaaga tcgctggcaa
     1321 gctgaaatcg gcgaaccggc acgaggccgc ctacgtcgcc cgccgacagg gctggatttg
     1381 accaacccag cggagggcgg cgggccccct cctccgactg gctccagcgc ctcttcggct
     1441 caggattccg gcaccgccgt ccgcagcccg cggcgcggcc cggcgccttc ggaccgtccg
     1501 ctggccgccg ccggaagggc gcgctcgtgc caggggctca ccggcagacc atggtcggcc
     1561 ggctggtcgg cccggttgac acttgcatct gcgtggcggg cggtcggcgc cacgtccggc
     1621 gccggcatgt ccctcctgcc gggcgcgccc gtcggcgggt ggtccgcgaa ggccgtggcg
     1681 cggctcgtca gctccgcgtc accacccttg cggacacgcg cgagagattg cccagaaacg
     1741 gcagcaccgc tgggacatgg ccttcgacgg cagtgcccgg ggaggcctct atgttcttcg
     1801 tcaagtgagg cggggtgttc ggggttcgta gaaggtgccg tcgcggagca tggcgaagag
     1861 cacgctgatg cggtgccggg cgaggcggag gagagcttga gtgtgggctt tgcctcttgc
     1921 ccggcacttg tcgtagtagc tgcgggaggt gggctcgtgc agggctgcga acgcggagag
     1981 aacatcgcgc gtttgagttc ccggttgccg cccagtggtg cgtgctcgcc ctggcacgag
     2041 gttccggacg acatgggcgt gggggcgagg ccggcgcatg agcccaggtg tccggcggta
     2101 gaaagctggt gccgtcgccg acggtgacca gcaaaacagc agcggtcctg accgccaccc
     2161 ccggcatcga gggcaggacc tgggaaagag ggtgctcttc cagcagagtg ttgatctggg
     2221 cttccagggc tcagcgctgt tcgtgacctg cggcgagctg ccggatcatg gacaggatga
     2281 cgatttcgag cgagccggtg ccgagaacca ccacggtctg ctcgtccacc gcgtcgaaga
     2341 cgtcgtcgat cagccttctc gccatcctgg gtgccttggg gccggagcgc ctcgacgcgc
     2401 ctggggcggc cgaccttacg cagcgtagca ggggagccgt agcgttccag cagccaggcc
     2461 atggcctggt gatcgaggcg cggtcccagt gcttgttcga agctggggtg gaactgggtg
     2521 agcaggccgc gtatccggtt ggaggtgcgg gtggcctcgg cggcgagatc ctggccgaag
     2581 ccgaccagca cggtcggctc tgcggtgatc tcgccggtca gcgccagtca gcggaggacg
     2641 tgggacatgg tgcgggccgc gtccgcgatg accgtggcgt ccttcgcgtc ggttgccgcc
     2701 tcaccggggt agagatcggc gatccgccgc atcgcgagtc cggtcaggca ggcgaccttg
     2761 cagcctgcgt tgcgagcgac ggtcagcggc agggccccga tcgaggcggg ctggtccacg
     2821 atcaccaggg ggtgccgaac ttcgcggtca gcttgccgaa gacggcccgc agcgccggcg
     2881 caatgttagg caactgcttg tcgaagactc tgccctccgc gtactgcccg gcattgcgcc
     2941 ggaccggacg gtggccgaac ctctcatcag tgtctccgac aacacctctc gggcccgatg
     3001 aacaccccct gcacccccag gtcatcgagg acagggggac acagccatgc cgggcccgga
     3061 ggagagcggc cctcttgtag gaccgcggaa accataacga ggggcggttt gctggcggac
     3121 gacttccggt gccgccgccg acccggtacg ggagggctgc ccttccgctc agcacagacg
     3181 cgcatgcgta tggcatcgct cacactaccg gggcgaagga ggttcacgct tccggccttg
     3241 gcggcggagc caaggggccg aggtggcaac cgcgtcacgg cccggggtgg cacctgtcac
     3301 ccggcaggac gcgcgcgtca agcggtcgga gtagtccgct ccagctgcga gtgtgacctg
     3361 cgccataaca aggatgctcg ggtgctggtt gtatcactgc atgtgcacga cggagggtcc
     3421 ggtgcgggcc ctccgtgaga ccagcgagag ggtgcgtcgt atgaccgatg cggcgattgt
     3481 cggcacgggc cccaacgggc tcgccgccgc tgtcacgctc ttgcgggcgg gcctcagcgt
     3541 cgaactctat gaggcgcacg acacgatcgg cggcggtctg cggaccgagt cgctcttcga
     3601 ctccgacgtg gtgcacgaca tctgttcggc ggtgcacccc atggcggcgg cgtctcgttt
     3661 cttccgggag ttcgacctgc ccgcccgcgg tgtcgacctg ctccagcccg agatccccta
     3721 cgcccacccg ctgctcggcg aggacaccgt cctcgcccac cgcgacctcg ccgaaaccag
     3781 tgcccacctc ggcgcggacg gcggacgatg gcggcgcctc atggcgccgc tcgccgcgca
     3841 cagccagggt gtcgtcgacc tgatcctgtc cggccagcgc agcgttcccg acgacctgtc
     3901 cgcgccgctc ctcttggcgc ccagagtcct ggcccacgcc acccgaatgc ccttccgcac
     3961 cgagcgggcc cgcgccttgc tcacgggtgt cgccgcgcac gccgtcggcc gactcccctc
     4021 gctcgctgcc agcgccgtct cgctcctttt gggacacctg gcgcacacct cgggatggcc
     4081 gctaccccgg ggcggcagcg cccgcatcgc ccaggccctc gcggccgaca tcgccgcgca
     4141 cggcggccgc gttcacaccg ggcagctgat caaggacctc ggcgagcttg agggcgtccc
     4201 cctggtgttg ctcgacacca gccccaaggg gcttctccag ctcgccggag accgactgcc
     4261 cagcccctac cgcagggccc tcgagcgctt tcggtacgga cctggggcgt ccaaggtcga
     4321 cttcctggtc tcagagccca tcccctggac cgacccccaa accggccggg cgggcaccgt
     4381 ccacctcggc ggtacccgcg cggacatgtt ccgtacggaa tcggcggccg tgcgcggcat
     4441 ccaggacccc gagccgttcg tgctcctcgt cgaccccgcg gcaaccgacc ccggccgggc
     4501 cgtcggcggc aaacgccccg tgtgggccta cgcccatgtc ccgaacggcg atccacggga
     4561 ccctgtcgac ctcgtccgcc gccgaatcga gcagtacgcg ccgggcttcg gcgacaccgt
     4621 catcgccgcc cgcggcctga gcgcggccca gtatgagacg tacaatccga actacgtcgg
     4681 cggcgacatc ggctccggag ccatgaccct ccgccagtca ctgctgcgcc ccaccgcgaa
     4741 gtgggacccc tacaaggtct cgctgcccgg cctgtacctc tgctcggctg ccaccccgcc
     4801 gggacctagc gtgcacggca tgtccggcca tctggcggcg ctctccgcgc tgcgccgcga
     4861 acacggcatc cgcgtcccgc cgccgctcgg cccgcacgcg tagcgctcgg tggaccggac
     4921 tacgccgtcc gcgctcccga caggccgcaa ccgcgcacgg cctgcacgac tccctcgccg
     4981 ccggctgagc caccgggccc tcaggacgcc ctcccagacg ttctgaaggc ccggcccacc
     5041 cgtgcgaacc gccgcccacc cacgaagcgc gcgaaagacc ggaccacgca catgccggtg
     5101 aagccagagc cggtgtaatt cgagtggtcc gcacctgcgc ccgccggctg catcatcacg
     5161 gaagtgcggg aagcccacct cggtgtgggc aaaatgggca cagtctctga gccttttcca
     5221 acctcacgct gaggcgtgat gaaggacgag gacggcctgg acgatcgctg tgatgcggtt
     5281 ggtgctgcag cggagtttcc gtaggagacg ccaggtcttc agggtggcca tggcctgctc
     5341 accgaggcag cggatcttgg catgtgtgct gttgtggcgg cgctgccacc gcttgaggcg
     5401 acggccgcgg aagggaaccc ggatggagcc gccggcgccc tgatatgcct tgtcggccca
     5461 gcacttgagg tcggcgtcgg cgagtgcttc gacgatgcca tgggtgcggg tggcggtcag
     5521 gtcgtgggtg gaaccgggca gcgcgggcga ggcccacagc aggcggccga acgggtcggt
     5581 gaggacgtgg acgttcatgc cgtggcgctt gtgcttgccc gagtagtacg gggtgtcggc
     5641 ggcgattcgg tcgatcggaa gcagggtgcc gtccaggatc acgaacgcct tggtccgcgc
     5701 gatgcgcata gcctcggcca gggtgggcgc gagggcggcc agtacgtcga cggcttcacg
     5761 tatgtagcgg tatgcggtcg cgatgccgat cccgaacccg gcggcgagct gggtgtaggt
     5821 gtcaccgcac cgcagatggg ccagggcgag gagagcctga cggccggcgg tcagacgtcg
     5881 ccaccgggtc ccgatctctc ctcgccgggc aacgagccgt ccggtcagga accgcagggt
     5941 gcgactggac aggtcgatag acgacgggta gacaagcata cgaagctcct ggcggacggt
     6001 ggatcttggt cgtgaacccg tctaccagga gcttcgtcgt tccgcagatc cgtccaacac
     6061 gcgaccagcg ccgtcaggtt ggaaacgact cactgccgca gtaaatgcgt acacctcgcc
     6121 tgcggcggcc ggagagtccg ccgcaggtcg cggtgatcac cgtagggctc cgccgcagta
     6181 aatccggtat ttactgcggc agacccctga cgagccatca ggcgacggaa gtccgataga
     6241 cagaccccga aaggaaccac cactaccgct atccgctgta ccgctggccc tcacgaagaa
     6301 cgcagccccg agcgttacac agcgctacat gtccgttcgc cgtagatata cgagaacagg
     6361 gcctacacgg gtgcctcccc ccaacggcca gctgaccccg acggaacgga cagtcaaccg
     6421 ggcactggcc acgccccgag ctcctgtcga acgcggtatg gcacggctca agtcctggcg
     6481 aatcttccga agatcgcggt gcagcccgaa ccccatgacg tcaatcgcca aagccgtctt
     6541 caccctcaaa agacaacgct gaaaacgctc attgagcctc ttcagagtgg gcaccggacg
     6601 ggtgacgggt ccgcgtgaag ggtgggctgc gctgagcgcg atcgcgcagg tcgatcggct
     6661 gtcaacggca gttggaccct cccccttgtg gggtcaagtt gaaaaggctc aatgagagcc
     6721 gcgcatgcca ccacgccggc ctctgagaaa tgagcgaccg aggacagccg gaaattacct
     6781 ccaggaggct cggccccaac cggtggtgct tgggcgccac ggtcggcagg accggcgggc
     6841 gaccatgtgc tcagacctcc cgccaggccg gggctgtgcc ctgcggcccg tcacgagccg
     6901 tggcgcgggc gcggctcgca ggactgaatg agcttcttca actcgagcct cggtcgagga
     6961 cagccaacag gggctggcct gcacggacgc tttcgacgcg ttccctgatc ggtgggcgag
     7021 ttgaagaagc tcgctgaggg ccgaccggtt cgggacatct ggacaaacca gaagtcgacg
     7081 ctctcccagc tttgttctag ctggtcagcg agaaaagcat gactaatgtc atcgtcaact
     7141 ttgcgccagt gtcgaatcag cgccgacggt caatatggac acgccctttc ttgtcgtgat
     7201 gtacgtcacg tcgacagggc ttgatttcaa cctttgtaat attcatatag tggtgcttgc
     7261 aagggggagg ttgaacgatc gttggcgagc ggctgtcgaa tcagggaact cagtcgatgc
     7321 tcggaatttc ggccatcaat ggggtggcct ttaaggtctg ttcatttcat gggcggtgtt
     7381 ccgctagagc gttctgtact gctccatgtt gggccgcact tgcctttcat tcgcgaaatg
     7441 agtgcatttc tgtcggcatc taatcgcgtt cggtcggtga cgctttcaat accccaacgc
     7501 cttgatcgaa atcaacagga ggaatggaac atgggcaaga tagccgcctt tatgaaccag
     7561 caggagctgg gctccctcga ggacggtctg acgaagaccg acggcacccc cgtcatggcc
     7621 accccggccg tggcggttcc ggtcaccgtc aagatcacgg ctggcatcgt cgccttcacc
     7681 atcccggtgg gcgcgtacgt cgccggccgt gtcgtcaact aaaggccgtc aggcattccg
     7741 agcatcgtcg aagaggagat cagaatgact cacatcatcg aaagcgtgcg gtcgatcacg
     7801 gagcccactc tgacggaggg ccagaaggac cggctcaccc gcaccatgcc ggtcgccttc
     7861 acccccgccg ttctcgttac caccccgtac gcgggcggcg gcctcgcggc cggcgctgcc
     7921 gtggtcggcg cctatgagct cggccgtgcc gcgggcggca agtagagagc accgtcagta
     7981 gccccttgtc atgagggtga acttctgaag ccgctcggag agtgaactct gcgcgcaaga
     8041 ggcgagctgt cgggccgcgc cggaatcgcg gcccgacagc tttcacatat tagctcaccc
     8101 catggttcag tcgtgaggaa tctcctgtga gacgaattaa gaagcttgcc tcgtcgcttt
     8161 tctctccggt gccgatatcc cgggaccagg ctctgggtat gtctgagcgg ctttccgcgc
     8221 tcactactct ctcctcctcc ctcgaatacc tgcaccagca caagcagatg gatcctggtg
     8281 gtctcaacga ctgggaaatc atgaaggagg cgccggagac caagaacctg gccatccgcc
     8341 gactcaccga ggccgtcagc ggcaaaaagg tgacggtggc gatacacacc gcccgggcgg
     8401 ctgtgagtct ggggctcctc ttttcgggca gcagcagatg gcgcggggcc ggaagtctct
     8461 tcctcacggc ttccacctct ctcttgcaga cccgccaccg ctacggcacg gacggttcgg
     8521 accaggtggc gaacctggtg cagaccgcca ctggccttgc ccgcctctcg aagagtccgc
     8581 agaccaagga cgcgctcctc tggtacgtgg cactgcaggc gagcctgtcc tacgcggcct
     8641 ccgggtgggt caaactcctc ggcgataagt ggcgcgacgg ctcggccctc cccggtgtga
     8701 tgcgcacccg tacctacggg ttcgagagcg cctaccacct cacccagcgc tatccgaaga
     8761 ctgcgaagta cctgcagtac ggcgtgctcg cgatggaatg cctcttcccg gttgtttacc
     8821 tcgccggagg caagctggcc cgtccgatga tcgccgcagc gggcagcttc cacgtggcca
     8881 acggcttcgt catgggactc ggccgcttca tgacggcctt ccccgccatg catcccatgg
     8941 tcgcctacac cacggctccc aagtcccacc cgtcggtagc gggacgcgac gaccgcgcgg
     9001 tgaaggccgc ggccctgctg cttgccggcg gtgtcgtcgc cgccgctgcg gtcgccgccc
     9061 agcgccgtgc cgtagtcctc gaaggctggc ccacgtcgcg caagtgcgtc acccggcatg
     9121 gcaacgaact catgtacgaa acgggtggcc gggacgacga gacggatgcg ccgatgctcg
     9181 tcttctgtgc cggactcacc tcgacgtccg agcacttcgc ctggatcacc gagcggttcg
     9241 tgaacgactc tgacagcgcg gtcgtctcct acgcgcgagc cgggtacgcg ggcagcctcc
     9301 ggcgtaccaa tgcgcctttc gtcctggagg aatccgtcga cgacctggag gacctgatcc
     9361 gcgccacggt gcccgggcac cgcaaggcgg tactggtcgg gcactccctg ggcggagagc
     9421 tgatccgccg ggtctcggcc cgcattccgg accgtgtcgc cggcctgatc tacctcgacc
     9481 cgtcccaccc tgccgagttg ctgcgctccg agcgccagag cgagggggcg aaggacctca
     9541 atgacaacat cacgcacgct gcccgctacc tctccatggg caccggcatc ctcatgagcc
     9601 gccctgggtg gctcgacagg ctccctgcga actaccggga caaggtgttc gcccagtaca
     9661 ccgacgcccg gctgtgggcg gcggggaagc gcgaatgggc ggcggtcgag aaggacttca
     9721 ggtccttcga tggtgacctg ccacaggtgg cggcacccgc gctgatcatc gcggcgcaac
     9781 agaccgtcga ccaagacccc gaacagctcc tgatgtacaa cgagctcgcc cgggcgcacc
     9841 agcgcggcga cgtcaaggtc gtcgaagggg ccgaccacga ctccctgctg accaacgcgc
     9901 tcttcgccca caagacggtg cagcacatga ccgccttcat gaaggacagc acgggcggcg
     9961 aacacagcga gtccgtcatc gacgagcaag gagcaccgaa gtgaacccag cagccctggt
    10021 caaggacgcg ttcaccggtc ccggttccct ggcgcgcgtc gtgggcgcgg ggatgctggt
    10081 ggcgaccgtc gccgcgcagc acccgcaccc ggcgttcgac cgggtccgct cccgagacct
    10141 gttctccgtc gttccgaact ggaagttctt cgcaccgaac ccggcgaccc acgacttcca
    10201 ctatgtctac cgcactctcg accacacggg cgggacgtcc gagtggagtg agatcgacat
    10261 gatcgagccc aggaagatgc accaggcgtt ctggttcgcc acccgccgcc cggagaaatc
    10321 cgtgttcgac ctgtgcacgg ccatccttca gcaggccacc aagaccggcg cgacggaggt
    10381 gcagaagacc tcctcctacc gggtcctcgc cgagttcatt cgcaagacga tccgcgacca
    10441 gcagggcgat gagaacgtca agggtttcca gttcgcggtc gtacgggccg cgggccacga
    10501 caccaatgag gaacccgagc ccctgttcat ctccccctac accccgatgc gccggttcgg
    10561 cccgcacctc gcggcggcct gaacaccacc gggaaccacc gccacaccga gcaccagtgt
    10621 tggagaacgc gccatgacca ccccccccat ccctgccgac gacttcgtcg gctatctccg
    10681 cacctacacc gccgacgcgt tcgcctccga acagagcgtg gaggacgtct gggtccgcta
    10741 ccacgtgccg aacgccactc atgtggtcaa ccggaagcag cgtgaccggg ccggcgtcct
    10801 gaagaacctc tcgcagtggc gggcggacca gacgccgtac gagctgtgga tccacgatgt
    10861 cgtggtggac ggctcgcgcg ccgccgcccg ttacaccatc agcaagcctt tggtgatgca
    10921 ggtgcggcac aacaccgaga cagtgatgtt cgccgacctc gctgacgacg gacgcgtagc
    10981 gtcgaccgtc accaccgcgc ggtcgaccta cggatggggc agctccgacg ggacctggga
    11041 cagcggtgcg ggctccgtcg gtgtgccgaa gggtgctccg acggacccgg cggcctacct
    11101 cacgaccttc ggggagctgg ccaaggaccc ggacgcgtcc ctcgaagagg catacgaccg
    11161 gttccacacc cccgactccg tccagttcat caacggcact tcgctgcagc gcccggcagc
    11221 cctgcaggcc ctcgccgccg cccgcgacaa aggccccgac tacaccctgg acatccacgc
    11281 ggcgctctcc caggggaacc gattcgcggt ccgttactcc atggtgccct cgcggcacgg
    11341 acgcccggcg agcgagatgg aggtcttcac cttcggcgag ctggccgacg acgggcgcgt
    11401 ccgcctcctc cgctcagcaa tgcagacccg gagcggctac tggcccacct gacccgcgcc
    11461 ggaccacgcc ggccccggac atcgcccaag ggaaagtgac actccgcatg accagcacag
    11521 ttgcagacat cggctacggc acgcagttcg ccacgtggta cgaccggatc tttcccaagg
    11581 gaggcgacgc gcaggccacg gcagacgtcc tggccgggct ccaccccgac ccgggcgccg
    11641 ggaccctcga gttcggggtc ggcacggggc gcatcgccct tccgctctcc cagaagaccg
    11701 gccaggtgat gggagtcgac tcgtccccgg aaatgctcgc ccagctggcc gaggccggga
    11761 gcgagaacgt cgtcccggtg catgcggaca tccgcaccta taccgacacc cgccagtacg
    11821 gcctggtgta cgtggtgtgc ggaacgctgt cgatgctgct cagtgccgag gaccagcagg
    11881 aggcggtgtg ccgcgccgcc gaactgttga cccccggtgg ccggctggtg gtggagaccg
    11941 gaaacctccc cgcggttcag gcgctccacg acggccaggc ccgctgcact ctcttcacgc
    12001 cctacccgga gccggggacg gggctgcaga cccactccac gctctcctcc gagatctggc
    12061 agtgctccca catctggtac gagtccgacg gcagcacccg agtgggcagt gaactggcgc
    12121 gcctcacgac ccccgacgag gtcgaccggt acgcggcggt ggccgggctc gcccccgaag
    12181 gccgctacgc cgactggagc ctctcctcgt acaccgagca gtacccgatg ttcctgacca
    12241 cgtacaccaa gaacgcctga gccacgccgg tgtacgccag cagtacaaga gaggatccac
    12301 tgatgacagc agaccgtgcg gtgtggttcg cggtcgcgtt cgtcttcgcc gcgccgagca
    12361 tgtccgcgtt tttccgtgag gacgggcggt tcaccagacg ggcggtggct ctgggcgtcg
    12421 tcttcgccac gagtgtcgcg gcgatcgtgg ccatcgcctt cgggcaggcc ccgtcgtgaa
    12481 cttctccgcg atcgtccgac ggcacagggt gctccttatc gtcggcatcc tcctcgggct
    12541 gggcggcacc gccgcgaccc tcgtccagcc gctcctcatc ggcgacctga tcgaggctgt
    12601 cgccctggac cagtcggtga cctggccgat cctgtccatc gccgccctgt ttgtcgccga
    12661 cgcggtcctc ggcgccaccc acttctacct gatcggccgg gcgggcgagg acatagtcct
    12721 cgacatgcgc tccaccctca cagggcgttt gctccactcg cgaatacggg cctttcacaa
    12781 gctcgagcac ggtgatgtgt tcacccgcac cgtctccgac acctcgctcg cccgagtcgc
    12841 cctgtcttcg tcggtcgccc aaatgatcac cgccggtttt accaccgtcg gctgcatcgc
    12901 cgtcatggcg tggatcgact ggcaactgct cctggtcacc gcgggctgcc tcggtgtcgc
    12961 ctccgcattg gcgctgatct tggccagagc ggtccgaaag gccgcggtcc agaaccggga
    13021 ggacaccagc gactacggct ctgggctcct tcgggtcctc ggcgcgctca ccaccgtcaa
    13081 ggcctccggc gccgagcgcc gcgaagccga gcggatcgag acgctcgcca aggccgcgcg
    13141 cggcagcggg atccgcgtga cccgtctgtc ggcgatgctg atgcccgcga tgaacgtggg
    13201 cacgcaggtc tccctcgcgg tggtgatcgc ctgggggatg gcgcgcacgg ccaccggagc
    13261 actctccgct gcggacctca cctccttcat catgtacctc ttctacctcg tctcgccgct
    13321 ggtgatgttc ttcatgtcgc tgggggagct gcagcagggc cgggccgcca tcgaccgtgt
    13381 caacgacctc gccaccgtcg accaggagac cgccgagatc tctgcgggcc tcgtggcgac
    13441 gccgaacctg gacgagtccg acgacttccc cgccgttgag ttccggaacg tcaccttcag
    13501 ctactccgac accgcttcgc cgaccctcga cgacgtctcg ttcacgctcc ccgccaccgg
    13561 catcaccgcg atcgtcgggc cgtcaggcgc cggtaaatcc acactgttcc atctgatcga
    13621 acgcttctac gacccagaca agggggggat ccggctcttc ggcgaagaca tctccctcct
    13681 gccgctccgg gaactccgct cccgcatcgg cctggtcgag cagggctccc cgctcatgcg
    13741 gggaacggtc cgcgagaacc tcgtctacac gcggcccgac gccacagcgg aggagatcgc
    13801 ccaggccgtg gaggcggccc atctcaccga cgtcatcgcc accctgccag acggcctcga
    13861 cactgccctc ggagagggcg gcacagggct ctccggcggc cagcggcagc gcctggccat
    13921 cgcccgggcc ctgctgaccc gccctgcggt cctgcttctc gacgaggcca cgtcaaacct
    13981 cgactcggac tccgagaccg ccctgcgctc cgcccttacc accatcggcg ccacctgcca
    14041 ggtcctcacg atcgcccatc gcgtctccac caccgtgggc gccgaccaca tcctcgtgat
    14101 cgaggaaggc cgactgaggg cccgcggcag ccacccagag ctgatggccg gcgaccccac
    14161 ctaccgacga ctggccagcc agcagttggc ccccctcgga tccgcatgac gaccgacccg
    14221 tcggaacctg ctgctccagc atggcaggat gcggacactg accgcactga cacacctgcg
    14281 aagaggtgca catgcccgtg atcctgtgga agcgctatgg cttggtgttc gccgccggcg
    14341 ccctgtgcct cgtggtgaac gcacagggga tcggcccgct ttggtacacc ggcgtggcgc
    14401 tgctgatcat cggccaggtc atgcgcgtga cactgatccg tgcccagcgc ccgcccctgg
    14461 accgcgagcc ggtccttctc cgactccccg tcaccggacg ctggctggcg ctcaacggcc
    14521 cggcgacgaa ggtccccagc cacacgcaca gccacgcgca gacgtacgcc atcgatctta
    14581 agtacgtacc cgagggcaga ggtgagcgga gcgcgccgca ggccccaccc ttccgctggc
    14641 tctggccact cgcccggagc cctcgcctct accccacctt cggcatgccg ctgctcgcgc
    14701 ccgccgacgg agtcgtcgtc gcgactgcgg gccgacaacg cgaccacctg tcgcgcatgt
    14761 cgttccccgc cttcctcttc ctctatctgg aaggcttcgt tcgcagtctc ggctggccgt
    14821 gccacctcat gggcaaccac atcatcctgg acctcggcga cggcgcgtac gcggctttcg
    14881 cacatctgcg acgcggctcg ctgcgggtgg ctccgggcga ccgggtgacg gctggccagg
    14941 agatggctga atgcggcaac tccggcaact cgtccgagcc gcacctgcac ttccagctca
    15001 tggacggccc cgacgtgatg acggcgcgag gtgtgccgtt cgcgtggtcc tatcgcgatg
    15061 acgacggcgt ggaacacacc ggtgtgcccg aggacaccac gcactgcgtc cccgtcgcac
    15121 cgctaccgga gcgctccgca tgacgtgcgg gctgtccacg ggacggcctg tggctcggca
    15181 ggggctctca gcctgccgag gtgatcgggc cttgacccct gatgttgaac acacgagaca
    15241 ctggatcctg aggatctgag aacggacatc tcgtggtcat gaagaactac ccgccgcagt
    15301 tcaaggcgga tgcggtcgcg ctgtacgagt cgcggccgga agcgacgatc aggtcggtcg
    15361 ccgccgatct ggggatcaac ccggagacgc tgcgggactg ggtccgggcg gccggggtga
    15421 gccgtccccg ggggcggtgg acgcaggaac cagccctgcc gtcggtgccg ctggaggccg
    15481 agaacgccgc cttgcggaag aaggtgcgtg agctggagga ggaacgggag atactgcgca
    15541 aagcggcgaa gtatttcgcc ggggagacgc gctggtgaac ccgctccagt gtgtcgccga
    15601 cctccagcgc cgttacggcg taaagcggct ctgcagcatc ctcggcgtga gccgctcgag
    15661 cttctagtac tggcgccgga cggctacgga ccgggccgcc cggcaggggg ccgacgcccg
    15721 tctggccgcc cggatacggg cggtgcaccg gaaatcggac ggcacctacg gagccccgag
    15781 gatcaccgcc gagctccgct aagggctgtc ccgtaactgc tggtcacaga tgagatgatc
    15841 ttggcgtggc tggtgtgatc acggcgtcgg agccgtcctg gattgccccg ttcagcgggc
    15901 tgagcacgag gcagttcggg aagctggtga cggtgctgcg tcgcgagggt gcggacgcgg
    15961 tccgcaaggg ccggccgtgg agcctgcccc tggaggaccg ggcactgctg gtcgcggcgt
    16021 actggcgcac aaacctgacc atgcggcagc tcgccccgct gttcggggtg tcgaagtcgg
    16081 cggcggaccg gatcatcgac cacctcggac cgatgctcgc gctccagccc cgcaagcgat
    16141 tcgccaagga caccgtgctc atcgtggacg gcacactggt ccccacccgg gatcacacca
    16201 tcgccgagcg gtcgaagaac taccggtact ccaccaacca ccaggtcgtc atcgacgccg
    16261 acacccgcct ggtcgtggtg gtcggacggc cgctcgccgg caaccgcaac gactgcaagg
    16321 cgtgggagga atccggcgcc aaggccgccg tcggcaagac cctcacgatc gccgacggcg
    16381 gctacccggg caccggactc gtcatcccgc accgccgaga gcgcggacag gccggactcc
    16441 cggactggaa agaggaacac aacaagtccc acaagcaggt ccgagcccgc gtcgagcacg
    16501 tcttcgcccg catgaagacc tggaagatcc tccgcgactg ccgcctgaag ggcgacggcg
    16561 tccatcacgc catgctcggc atcgcccgca tgcacaacct cgccctcacc ggataaaccg
    16621 gcggtccgca caacggcctc ccccataccc gaggcgaccc gaagatcatt tgcgggacaa
    16681 cccttaggcg tgaagtaacg gccgagttcg gtgagcctcc gatcgcggag ttcttcagag
    16741 ggctcggtgt acacgacccg tattgtgctc cgcctggctc ccgactgcgg gcagcgccac
    16801 gccccttccg ccggtgccga caggcagcgg tcggcttcgc ggaggaccac ttcggcagag
    16861 cttcggggcc gtgacagcgc ctggtccgtt cgggcggctc ttctacatgg tgatgtcgtc
    16921 gtcgctcctc acagcacgtc gtacctcctg ttcaaggccg aaacggttca actgtgcggt
    16981 tcgggcgtag tgggtgatgg cagcttgcgc tgttgcagct gcttcgaacc acgcccgcct
    17041 ctggcaggtg cgctcgcctt cgctatggag cgcgtacagc ttctcgcgtt caagatcggc
    17101 ggaccgctgc agggcaagca gttcggcggg gagaccgctt ccgctcggtt cgtgctcaag
    17161 ggttggcgga tcgggtactg ctgtgttttc agtgtgagct ccagtcgttt cgacggccgc
    17221 ggtcgacggc tgggcggaaa ccagaggttg cggcttctgc gcataagtac cgatcttgtg
    17281 cgtgctctgg tttgtgctcg tggtgccctg cgcggcgcgt tcctcatgga gggaggggcg
    17341 ccgaccgtcc gactgcgagg gaagaccaag ggttgctggt cgatgtgcct gggatgcgct
    17401 gccgctgggc agtccggtgg cgggcaggtg tgtgcggagg ggcggtggat cgtcgactgt
    17461 aagggtcagc tctacgccgt aaatgccgcg gcgctccagt ggtgcactgg ggacccaggt
    17521 ttggtcgccg aatttgcagg tggtgtcggc gtctctgccc gagaagtgga tgaaccgttc
    17581 cgggttcggt agagactcga ttccgtgaaa ggattgagtc atggcacgcc cctcctccta
    17641 tccccttgag ctgcgcaaac gagcggtccg tatggtcgcc gaggtccgcg gtgactaccc
    17701 gaacgagtcg gctgcgctgc gggcggtcgc ccagaagctc ggcatcggct cggccgagac
    17761 cctgcggaac tgggtgaagc gcgacgagat cgactccggt cagcgtccgg ggacgaccac
    17821 ggaggagtcc gcccagatca aggcgatgaa gaaggagatt gccgagctga agcgggcgaa
    17881 cgacatcctg aaggctgcgg caagtttctt cgcggccgag ctcgaccggc cacacacacg
    17941 ctcgtagcgt tcatcgacga gcaccgggcc cgcttcggcg gtgtcgagcc gatctgccgc
    18001 gtgctcaccg agcacgactg caagatcgcc ccttctacct attacgccca ccataaacgg
    18061 cttcaggtcc cgtcagcccg caccatccgc gacgccgagc tgaagccgct catccaacag
    18121 atcttcgacg ccaactatcg tgtctacggg gccaggaagg tctggcgtga gctgcaccga
    18181 cagggccact tcgtggcccg gtgcaccgtc gaacgcctca tgcgcgagct gggcgtcgca
    18241 ggtgccgtcc gcgggaagaa gatcatcacc acgatcccgg acggcagcgt cgagcgggca
    18301 ccggacctgc tggaccgcaa cttcgtcgcg ccggccccca accgctgctg ggtcgccgac
    18361 ttcactcacg tcagcacctg gtcgggagtc gtctacgtcg ccttcgtcgt ggacaccttc
    18421 tcccgccgga tcgtcggctg gtccgcagcc ctgtcgaagg agacacggct cgtcctggac
    18481 gcgctggaca tggccctgtg gcaacgcgac cgcgacgaaa agccgcatca gcggggcgag
    18541 ttgatacatc actccgatgc cggttcgcaa tacacgagct tccggctcgc cgagcacctg
    18601 gacgccgccg gcatcgcggc ctcgatcggc tccgtcggcg acgcctacga caacgccctc
    18661 atggagtcca cgatcggcct gttcaaaacc gagctgatca agcccggaca gccctggagg
    18721 acgctctccc aggttgagct ggccaccgcg gaatggatcg actggtactg ccaccgccga
    18781 ctccacggtg aaatagggca catcccaccc gtcgaatacg agaccaacta ctacctcgcc
    18841 accacgaaac cccaggtcac aaccaaaatc tgaggtctct accgaacccg gggcggttca
    18901 ggatgtgctc ttccggggct tgttgctctt cgggaggtgc ggcttcgtcg gcacggtatt
    18961 cggtccaccg ggtcttgtcg tccgcgggca cccacatgta cgcgccagac cgccggtcct
    19021 tacgggaagc ggcgaacagt ggggtgacgc taccggtggc agcgccgatc agggtctgcc
    19081 cgacggtgac ccctcgggta ttgcccttgt ggcttgtcca gcccgtgcct tcgtcgtcca
    19141 gacggcgagg ggagtcccag tgcaggtgca ttttcccgca acctgtcttc actcgggcat
    19201 ctggacacgg ccgctcagca cccgcggtgc accgccactc gaccagaact cggaagccgc
    19261 ccatgaggga aatgtctacc gcagagctga tctccttcca ggacatctct ctggtcagca
    19321 tccagttcga acggtcaatt agatggaacc tgtcgtcatg cgtgatccag ttggcgatca
    19381 gcccggagtc ggcatgggcc ttcgaccggc gcagtacagt acccgcgctg gcgttgtagt
    19441 actgggcttc gcatcccagg ctcactccgt caggtccgtt gatcgtgacg tcgggtctgg
    19501 acgtccggtt cctggccgtc gcctccactt ggacctcgag gccggccgac tccccggtgc
    19561 gggccaggaa gtccttcatg gccttgtgcc ggtcactttc ttccgaagtc atttggtgtg
    19621 ccgtgggcaa gtgcgcagcg cgcaggcgca ggacgccgtc aggaccgcga tgcttccgca
    19681 cgtacatgtg cgggctcttg accccgctca gttcagcctt gcaccgctga ccttggctgc
    19741 tctccacgca gacgagaagt tcgcggtccc tctcagccac aggctgcagc aggctctcga
    19801 tcaattcgga taggtgcggc cacgcgccgc ccaggccgag gtcttccttg tcgagttcga
    19861 gggtccgctg gagaccggaa tgccacacgc cgtttgccac ttacattcct cactcgatag
    19921 cagcgcacag gagactgttc cggcaaggta gggactgcca ctgacaagga gtccagatcg
    19981 gcattgcatt cagttctaac agcgaactga cgtacgccgc atagagtttt cggtcgaacc
    20041 tcgacccccg gattgactga gctcacacgt actcgcgcac gtggggtgaa aggatcctcg
    20101 tgggccaacg ggaggccgcg ggctactctg atccgatcct gtgcggggcc tgggtaacca
    20161 ggtgttgtcc gggggtagag cgtggcgcct gagcggacag gaccaatcaa tcggatggcg
    20221 tgacgttgct ggtggcagag tcgttgtccc gctcgtgaag atcaccttga tgcgtaccgc
    20281 cgccgccctc gccgtggccg cccttctccc catctcgtcc tccgccgccg ccgagacgag
    20341 cgaggctggc ccgacggtgt tgccgatcga cgctgccgtg gccgcgctcc agtccgccga
    20401 cgaatccagg accggatact cccggaccgc gttcaagcac tggaccgccg gcgacaaccc
    20461 cacggacggc tgcaacaccc gccaggaagt cctcatcgcc gaggccacca tcgcgcccga
    20521 ggtcgggccc cggtgcgcca tcaccggggg cacctggttc tcgtactacg acgaggtcac
    20581 cg
//
