LOCUS       NZ_CP106650            10399 bp    DNA     linear   CON 16-MAY-2024
DEFINITION  Streptomyces sp. GBA 94-10 4N24 strain GBA 94-10 chromosome,
            complete genome.
ACCESSION   NZ_CP106650
VERSION     NZ_CP106650.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. GBA 94-10 4N24
  ORGANISM  Streptomyces sp. GBA 94-10 4N24
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7025021)
  AUTHORS   Ian,E., Malko,D.B., Sekurova,O.N., Bredholt,H., Ruckert,C.,
            Borisova,M.E., Albersmeier,A., Kalinowski,J., Gelfand,M.S. and
            Zotchev,S.B.
  TITLE     Genomics of sponge-associated Streptomyces spp. closely related to
            Streptomyces albus J1074: insights into marine adaptation and
            secondary metabolite biosynthesis potential
  JOURNAL   PLoS One 9 (5), e96719 (2014)
   PUBMED   24819608
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025021)
  AUTHORS   Ruckert,C., Busche,T. and Kalinowski,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2022) CeBiTec, Uni Bielefeld, Sequenz 1, Bielefeld
            33615, Germany
REFERENCE   3  (bases 1 to 7025021)
  AUTHORS   Ian,E., Sekurova,O.N., Malko,D.B., Ruckert,C., Borisova,M.E.,
            Albersmeier,A., Kalinowski,J., Gelfand,M.S. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2013) CeBiTec, Bielefeld University,
            Universitaetsstr. 27, Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-NOV-2021
            Assembly Method                   :: flye v. 2.9-b1768
            Assembly Name                     :: GB94_v2
            Genome Coverage                   :: 229.4x
            Sequencing Technology :: Illumina MiSeq;Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000495635.2-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 06:31:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,298
            CDSs (total)                      :: 6,204
            Genes (coding)                    :: 5,988
            CDSs (with protein)               :: 5,988
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 100 of 216
            Pseudo Genes (incomplete)         :: 164 of 216
            Pseudo Genes (internal stop)      :: 24 of 216
            Pseudo Genes (multiple problems)  :: 67 of 216
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:13:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5645529
            Orig. end                         :: 5655928
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP106650.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Wittmann Lab, Saarland
            University, Germany.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="21"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            266..991
                     /locus_tag="B591_RS24985"
                     /old_locus_tag="B591_24773"
     CDS             266..991
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412186.1"
                     /locus_tag="B591_RS24985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_24773"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_023422978.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFDKALAAFPHRDRIEVVHRSFELDP
                     ARPKGDPVLVLPMLAKKYGMSEAQAAEAERNLGQNAAAEGLPYLTEGRDHGSTFDMHRL
                     LHLAKARGRQDELLAVLYRANFAETETVFGDDERLVALAVEAGLAEAEAREVLADEQRY
                     AAEVRADERAATELGASGVPFFVLDRRYGVSGAQPADVFTQALTQAWESHVPASGLATP
                     AGAEGGEACGPDGCEVPKG"
     gene            1121..2170
                     /locus_tag="B591_RS24990"
                     /old_locus_tag="B591_24778"
     CDS             1121..2170
                     /NRPS_PKS="Domain: Aminotran_5 (55-343). E-value: 1.7e-23.
                     Score: 75.0. Matches aSDomain:
                     nrpspksdomains_B591_RS24990_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018895195.1"
                     /locus_tag="B591_RS24990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_24778"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_023422979.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7e-18, bitscore:
                     63.2, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLARSLAEDEFTSSLTHLATASTGLLPLSAARAVHEAVDAAAAG
                     TPPDMFGARDAARASFARLVGVPVTRVATGSSVATFTGLIAAALPDGAEVLTADGDFSS
                     LVNPFHVRPGLTVRTVPLERIAEHVTEATALVAVSAVQSADGRRADLTAVREAARAHGA
                     RTLVDASQAAGWDTFEADAYDYTVTVGFKWLLAPKGTAFLTVPEVPGALPPLLAGWVAG
                     DSPFDACYGPVTDLAPDARRYDESPAPLVWTGAQHALALLERIGTEAVGAHDVALADRF
                     RERLTRAGHAPVPAPGSAIVSVPGLGERAAALGERGITVSDRAGMLRAAFHLYNTERDV
                     DRLVAALGG"
     aSDomain        1286..2149
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B591_RS24990_Aminotran_5.1"
                     /evalue="1.70E-23"
                     /label="B591_RS24990_Aminotran_5.1"
                     /locus_tag="B591_RS24990"
                     /protein_end="343"
                     /protein_start="55"
                     /score="75.0"
                     /tool="antismash"
                     /translation="AARASFARLVGVPVTRVATGSSVATFTGLIAAALPDGAEVLTADG
                     DFSSLVNPFHVRPGLTVRTVPLERIAEHVTEATALVAVSAVQSADGRRADLTAVREAAR
                     AHGARTLVDASQAAGWDTFEADAYDYTVTVGFKWLLAPKGTAFLTVPEVPGALPPLLAG
                     WVAGDSPFDACYGPVTDLAPDARRYDESPAPLVWTGAQHALALLERIGTEAVGAHDVAL
                     ADRFRERLTRAGHAPVPAPGSAIVSVPGLGERAAALGERGITVSDRAGMLRAAFHLYNT
                     ERDVDRL"
     gene            complement(2181..2693)
                     /locus_tag="B591_RS24995"
                     /old_locus_tag="B591_24783"
     CDS             complement(2181..2693)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018470511.1"
                     /locus_tag="B591_RS24995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_24783"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_023420015.1"
                     /transl_table=11
                     /translation="MNEATGPEQAPCTWPAERGAALPGGPVGHLVTRVARLHRTAAGRA
                     LRESGLYPGQEIVLGHLWEAGGPVRQSDLIKAAELDPSTVTKTLQRLEQSGHVTRAPDP
                     ADRRAVLVEATELSCALSRQVESAWAGLEEHALAGLTPADRAELLRLLAAVEANLCDRA
                     GDCPEAG"
     gene            2865..3926
                     /locus_tag="B591_RS25000"
                     /old_locus_tag="B591_24788"
     CDS             2865..3926
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947424.1"
                     /locus_tag="B591_RS25000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_24788"
                     /product="alkene reductase"
                     /protein_id="WP_023420016.1"
                     /transl_table=11
                     /translation="MSTAFEPLDLSGTKLPHRIAMAPMTRSRSGAAGVPTALMAEYYAQ
                     RASAALIVSEGIQPSAVGQGYPDTPGLHSAEQVTGWRAVTSAVHAAGGRIFAQLMHAGR
                     IGHPSLLPEGEINVAPSAVAATGQVYTAEGPEDFVTPRELTDEEVRATLADYVAAARNA
                     VDAGFDGVELHGANGYLIHQFLAPGSNRRADGWGGSVEGRIRFAAEVTEAVAAEIGAAR
                     TALRLSPGNAFNSIEEPEPEPEYTALVARIAPLGLAYLHVAEDVPARRALTLRLRGLFE
                     GPLMLNPADSPTGPEALALVEEGTADLVSYGQLFLANPDLPARLRAGGPFNEARRATMY
                     GGGAEGYTDYPAL"
     gene            complement(4101..4997)
                     /gene="thpD"
                     /locus_tag="B591_RS25005"
                     /old_locus_tag="B591_24793"
     CDS             complement(4101..4997)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010637026.1"
                     /locus_tag="B591_RS25005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_24793"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_023420017.1"
                     /sec_met_domain="PhyH (E-value: 5.7e-38, bitscore: 129.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVASERTDLYPSRTATEVTIPRKDPVVWSPQDAPGPITRSDLQS
                     YDENGFLAVEELITPEEVGVYRAELDRLVADPLIRADERAIVEPKSEKVRSVFEIHKIS
                     EVFAGLVRDERLLGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPR
                     MRAVSVSIALTENYDTNGGLMIMPGSHRTYVGCNGVTPKDNYKRSLQMQDAGIPSDEVL
                     TEFADRHGIKLFTGKAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENAAEEPFAA
                     PVRRPEFIGARDFTPVK"
     gene            complement(5001..5399)
                     /locus_tag="B591_RS25010"
                     /old_locus_tag="B591_24798"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947422.1"
                     /locus_tag="B591_RS25010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_24798"
                     /product="ectoine synthase"
                     /protein_id="WP_003947422.1"
                     /sec_met_domain="ectoine_synt (E-value: 9.9e-55, bitscore:
                     182.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIENTDRHVKSASGTWESKRIVLAKEKVGFSLHETILY
                     AGTETDMWYANHIEAVLCVEGEAELTNRETGETHWIEPGTMYLLNGHEKHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEED"
     gene            complement(5434..6705)
                     /gene="ectB"
                     /locus_tag="B591_RS25015"
                     /old_locus_tag="B591_24803"
     CDS             complement(5434..6705)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 8e-75.
                     Score: 243.9. Matches aSDomain:
                     nrpspksdomains_B591_RS25015_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="B591_RS25015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_24803"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_023420018.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.6e-79, bitscore:
                     264.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRSWPAVFDRAQGSYLHDEDGHTY
                     LDFFAGAGALNYGHNNPVLKRALIDYIERDGITHGLDMGTTAKRAFLETFQNVLLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTMQDFLWFERLLEDQGSGLNTPAAVIVETIQGEGGINVARP
                     EWLRALADLCKRRDMLLIVDDIQMGCGRTGPFFSFEEAGITPDIVTLSKSIGGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWSDGQMEKQTLARGEQVEQALT
                     AILDEHGGEAIVSVRGRGLVWGMEFADKSRAGAVSKRAFELGLLVETSGPESEVVKLLP
                     PLTVSPDELDEGLRILARAVRETA"
     aSDomain        complement(5608..6621)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B591_RS25015_Aminotran_3.1"
                     /evalue="8.00E-75"
                     /label="B591_RS25015_Aminotran_3.1"
                     /locus_tag="B591_RS25015"
                     /protein_end="366"
                     /protein_start="28"
                     /score="243.9"
                     /tool="antismash"
                     /translation="FDRAQGSYLHDEDGHTYLDFFAGAGALNYGHNNPVLKRALIDYIE
                     RDGITHGLDMGTTAKRAFLETFQNVLLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTMQDFLWFER
                     LLEDQGSGLNTPAAVIVETIQGEGGINVARPEWLRALADLCKRRDMLLIVDDIQMGCGR
                     TGPFFSFEEAGITPDIVTLSKSIGGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWSDGQMEKQTLARGEQVEQALTAILDEHGGEAIVSVRGRGLVWGMEFA"
     gene            complement(6822..7328)
                     /gene="ectA"
                     /locus_tag="B591_RS25020"
                     /old_locus_tag="B591_24808"
     CDS             complement(6822..7328)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412174.1"
                     /locus_tag="B591_RS25020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_24808"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_037787473.1"
                     /transl_table=11
                     /translation="MKEMPEDLRLDHPGIEDGAAIWRIARDSRTLDLNSSYSYLLWCRD
                     FAGTSVVARDAAGEPVGFVTGYFRPERPHTLVVWQVAVDASQRGRGLAAALLDGLTDRL
                     REKGRLDGIETTITPGNTPSERLFTSYAKRHGAELEREVLFGAGLFPDGGHDPEVLYRI
                     GPFTD"
     misc_feature    complement(7369..7381)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    7387..7401
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.91"
                     /tool="antismash"
     misc_feature    7389..7403
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.12"
                     /tool="antismash"
     misc_feature    complement(7395..7407)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     misc_feature    complement(7484..7502)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 18.88"
                     /tool="antismash"
     misc_feature    complement(7526..7541)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7536..7551
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7547..7562)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 18.42"
                     /tool="antismash"
     misc_feature    complement(7669..7682)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.78"
                     /tool="antismash"
     misc_feature    7669..7685
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            complement(7824..8939)
                     /locus_tag="B591_RS25025"
                     /old_locus_tag="B591_24813"
     CDS             complement(7824..8939)
                     /NRPS_PKS="Domain: Aminotran_5 (73-284). E-value: 3.8e-30.
                     Score: 96.9. Matches aSDomain:
                     nrpspksdomains_B591_RS25025_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947419.1"
                     /locus_tag="B591_RS25025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_24813"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_023420020.1"
                     /sec_met_domain="Aminotran_5 (E-value: 5.7e-23, bitscore:
                     79.9, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPLLDLTPLTAAHFANIERRVAALLSTAQDVVITQGEALLPLE
                     GCIRGVAGPDTTALNVITGPYGQTFGDWLRDCGARVVDLAVPYDSAVSADQVAEALAEH
                     PEIGFVSLVHAEAATGNTNPVAGIGRVVREHGALFMVDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWQRMADNPQAPRRSYLSLLDWKERWIDAGRTALLHAP
                     AQLEMVALQACVERIEDEGLETVIGRHTRAAAATRAGVKALGGGFAPYVREARHAAPVA
                     TTLRAPEGVDASELVARALAADPVLPLVAGGGALAKEMIRVNHYGTAAAGSTVHSCLSA
                     LGAALTDLTGRSVDPEAARRAAIAAWERPAG"
     aSDomain        complement(8088..8720)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B591_RS25025_Aminotran_5.1"
                     /evalue="3.80E-30"
                     /label="B591_RS25025_Aminotran_5.1"
                     /locus_tag="B591_RS25025"
                     /protein_end="284"
                     /protein_start="73"
                     /score="96.9"
                     /tool="antismash"
                     /translation="LRDCGARVVDLAVPYDSAVSADQVAEALAEHPEIGFVSLVHAEAA
                     TGNTNPVAGIGRVVREHGALFMVDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVS
                     AVSVSERAWQRMADNPQAPRRSYLSLLDWKERWIDAGRTALLHAPAQLEMVALQACVER
                     IEDEGLETVIGRHTRAAAATRAGVKALGGGFAPYVREARHAAPVATTL"
     gene            9014..10105
                     /locus_tag="B591_RS25030"
                     /old_locus_tag="B591_24818"
     CDS             9014..10105
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947418.1"
                     /locus_tag="B591_RS25030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_24818"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_037787474.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 7.2e-16, bitscore:
                     56.8, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEDMVLHVKGRILVGPEEARDELWVVGGRITFSRPAGAREVRTV
                     EGWALPGLVDAHCHVGLDRHGPVAADVAERQALTDRDAGALLLRDAGSPSDTRWMDERD
                     DLPRVIRAGRHIARTRRYIRNYAHEIEPDQLVAYVGQEARRGDGWVKLVGDWIDRGAGD
                     LTPCWPREAVEAAIAEAHRLGARVTAHCFAEDSLRDLVEAGIDCVEHATGLTEDTIPLF
                     AERGVAIVPTLVNIATFPDLAAGGEAKFPDWSAHMRRLYERRYDTVRAAWDAGIPVFVG
                     TDAGGSLPHGLVAAEVAELVTAGIPPLQALSATAWGARDWLGRPGLTEGAPGDLVVYAS
                     DPRADVRVLAAPRRVVVGGKVVA"
     misc_feature    10266..10281
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.95"
                     /tool="antismash"
ORIGIN
        1 ccagaccacc cccgaggcgc tgctgccgcc ggagctgcgg gacggcaacc cgctggggct
       61 gccccggcac gtggacgggc gggccgaacg cggggcctga gggcggccgg gggctgaccc
      121 gggccggtcc ggaatgcacc ggtccggccc cgcgttgcag cagagcggag gcaccctccg
      181 taccctggtg tccgcacgcc ccgggagacc gctcccggca cccccgacct gcccggtccc
      241 cgggccgagg agttgaaccg cagccatgcg cgtcgagatc tggtccgaca tcgcctgtcc
      301 ctggtgctac gtgggaaagg cgcggttcga caaggcgctc gccgccttcc cgcaccgcga
      361 ccggatcgag gtcgtgcacc gctccttcga gctggacccg gcccgcccca agggcgaccc
      421 cgtcctggtc ctgccgatgc tcgccaagaa gtacggcatg agcgaggccc aggccgccga
      481 ggccgagcgg aacctcgggc agaacgccgc cgccgagggc ctgccctacc tcaccgaggg
      541 ccgcgaccac ggcagcacct tcgacatgca ccggctgctc cacctcgcca aggcgcgcgg
      601 ccgccaggac gagctgctcg ccgtgctgta ccgcgccaac ttcgccgaga ccgagaccgt
      661 cttcggcgac gacgagcggc tcgtcgccct cgccgtcgag gccgggctgg cggaggccga
      721 ggcccgcgag gtcctcgccg acgagcagcg gtacgccgcc gaggtccgcg ccgacgagcg
      781 cgccgccacc gaactgggcg cgagcggcgt cccgttcttc gtcctcgacc ggcggtacgg
      841 cgtctcgggc gcccagcccg ccgacgtctt cacgcaggcc ctcacccagg catgggagag
      901 ccacgtcccc gcctccgggc tggccacccc ggccggcgcc gagggcggcg aggcctgcgg
      961 ccccgacggc tgcgaggtcc cgaagggctg accgcgcacc ggcaccggcc cggcccgcca
     1021 cccactccgg cgcggcggcg ggccggaccc gtaccaccag ggaccgttcg gcggccgccg
     1081 caccatggac ggctgccgcg cgagggtgca cagtggccgc atggatctcg cccgcagcct
     1141 ggccgaagac gagttcacgt cctccctgac gcacctggcg accgccagca ccggcctcct
     1201 gccgctgagc gcggcccggg ccgtgcacga ggcggtcgac gccgccgcgg ccggtacccc
     1261 gcccgacatg ttcggcgccc gggacgccgc ccgggcctcc ttcgcccggc tggtgggcgt
     1321 ccccgtcacc cgcgtcgcca ccggatcgtc ggtggcgacc ttcaccggcc tgatcgccgc
     1381 cgcgctgccc gacggcgccg aagtgctcac cgccgacggc gacttcagct cgctggtcaa
     1441 ccccttccac gtccggccgg gactgacggt gcgcaccgtg ccgctggagc ggatcgccga
     1501 gcacgtcacc gaggcgaccg ccctggtcgc ggtgagcgcc gtgcagtccg ccgacggccg
     1561 ccgcgccgac ctcaccgccg tccgcgaggc ggcccgcgcc cacggcgccc gcaccctggt
     1621 cgacgcctcc caggccgccg gctgggacac cttcgaggcc gacgcgtacg actacacggt
     1681 caccgtcggg ttcaagtggc tgctcgcccc gaagggcacc gccttcctca ccgtcccgga
     1741 ggtgcccggc gccctgcccc cgctcctcgc gggctgggtc gcgggcgact cccccttcga
     1801 cgcctgctac ggacccgtca ccgacctggc ccccgacgcg cgccgctacg acgagagtcc
     1861 cgccccgctc gtctggaccg gcgcacagca cgccctcgcc ctgctggagc ggatcgggac
     1921 cgaggcggtc ggcgcccacg acgtggcgct cgccgaccgg ttccgggagc ggctgacccg
     1981 cgccggacac gcccccgtgc cggcccccgg ctcggccatc gtctcggtgc cggggctggg
     2041 ggagcgggcg gcggccctcg gggagcgggg gatcaccgtc tccgaccggg ccgggatgct
     2101 ccgggccgcc ttccacctgt acaacaccga gagggacgtc gaccggctgg tggcggcgct
     2161 cggcggctga gccgcgcggg ctatccggcc tcggggcagt cgcccgcccg gtcgcagagg
     2221 ttggcctcca ccgccgccag caggcgcagc agttcggcgc ggtctgcggg ggtgaggccg
     2281 gccagggcgt gctcctccag ccccgcccag gcggactcga cctggcggct cagggcgcag
     2341 ctcagttcgg tcgcctcgac caggacggcc cgccggtcgg cggggtcggg ggcgcgggtg
     2401 acgtgcccgg actgctccag ccgctggagg gtcttggtca ccgtggaggg gtcgagttcg
     2461 gccgccttga tcaggtcgga ctggcggacc gggccgcccg cctcccagag gtggccgagg
     2521 acgatctcct ggcccgggta gaggcccgac tcgcgcagcg cccgcccggc cgcggtccgg
     2581 tgcagccgtg ccacccgggt gacgagatgg ccgacgggcc cgccgggcag ggccgcaccc
     2641 cgctcggcgg gccaggtgca gggggcctgc tcggggccgg tcgcctcgtt catccacgtc
     2701 tcccgctggg gtcgtcctcg tcccggtctc cggccgacta tacctttggt cggccaatca
     2761 atgggttaca gtgattcccg gcccaattga ttggccgacc atgcagtgtg tcggccggag
     2821 cccgctctgc ccggaaccac ccgttccccc actggagacc gcccgtgagc accgccttcg
     2881 agcccctcga cctgtccggc accaagctcc cgcaccgcat cgccatggct cccatgaccc
     2941 gcagccgctc cggcgcggcg ggcgtgccga cggcgctcat ggccgagtac tacgcgcagc
     3001 gcgcctcggc cgccctgatc gtcagcgagg gcatccagcc ctccgccgtc ggccaggggt
     3061 acccggacac ccccggcctg cattcggccg aacaggtgac gggatggcgc gcggtcacct
     3121 ccgccgtgca cgccgccgga ggccggatct tcgcccagct catgcacgcc ggccggatcg
     3181 gccacccctc cctgctcccg gagggcgaga tcaacgtcgc cccctccgcg gtcgccgcca
     3241 ccggccaggt gtacaccgcc gagggccccg aggacttcgt caccccgcgt gagctgaccg
     3301 acgaggaggt ccgcgccacc ctcgccgact acgtcgcggc cgcccgcaac gcggtcgacg
     3361 ccggtttcga cggggtcgag ctgcacggcg ccaacggtta tctgatccac cagttcctcg
     3421 cccccggctc caaccgccgc gccgacggct ggggcggctc ggtcgagggc cgcatccggt
     3481 tcgcggccga ggtcaccgag gcggtcgccg ccgagatcgg tgcggccagg accgccctgc
     3541 gcctgtcgcc cggcaacgcc ttcaacagca tcgaggagcc cgagccggag ccggagtaca
     3601 ccgcgctggt cgcccggatc gccccgctcg gcctcgccta tctgcacgtc gccgaggacg
     3661 tcccggcccg ccgggcgctc accctgcgcc tgcgcgggct cttcgagggc ccgctgatgc
     3721 tcaacccggc cgacagcccg accggccccg aggcgctggc cctggtcgag gagggcaccg
     3781 ccgacctggt cagctacggc cagctcttcc tggccaaccc cgacctgccc gcgcggctgc
     3841 gcgccggggg accgttcaac gaagcccggc gcgccacgat gtacggcggc ggggccgagg
     3901 ggtacaccga ctacccggcg ctctgaccgg cgcgcggggc gggaaaggcc cgaaaaacgc
     3961 aaccggaccg ggggcggccc gcctgtggcc gcccccggtc ccgtggcccg ggcgccgtgt
     4021 gcgccggtgc gtgacgagcc cgctgtcagc ggctcgtccg gtgtgcctgc tccacctggg
     4081 tgaagcggga cgtcaccggt tcacttcacc ggagtgaagt cacgcgcccc gatgaactcc
     4141 ggacgccgca ccggcgccgc gaagggctcc tcagcagcgt tctcgacgct gttgaagacg
     4201 atgaacacgt tgctgcgcgg gtacggggtg atgttgtccc cggagccgtg catggcgttg
     4261 cagtcgaacc aggtggccga cccggccttg cccgtgaaga gcttgatgcc gtgccggtcg
     4321 gcgaactcgg tcagcacctc gtcggagggg atgcccgcgt cctgcatctg gagggaccgc
     4381 ttgtagttgt ccttcggcgt cacgccgttg cacccgacgt aggtgcggtg cgaccccggc
     4441 atgatcatga ggccgccgtt ggtgtcgtag ttctcggtca gcgcgatcga gacggacacc
     4501 gcgcgcatcc tcggcagacc gtcctcggcg tgccaggtct cgaagtccga gtgccagtag
     4561 aagccggagg cgccgaagcc aggcttcacg ttgatccggg actggtggac gtagacgtcc
     4621 gagccgagga tctgccgggc gcggccgagc agccgctcgt cacggaccag gccggcgaag
     4681 acctcgctga tcttgtggat ctcgaagacc gagcggacct tctccgactt gggctcgacg
     4741 atcgcccgct cgtccgcccg gatgagcggg tcggcgacca gccggtccag ttcggcccgg
     4801 tagacgccga cctcctcggg ggtgatcagc tcctcgacgg cgaggaagcc gttctcgtcg
     4861 tacgactgca ggtcggaccg cgtaatgggt ccaggggcgt cctgcgggga ccacaccacg
     4921 gggtccttgc gcgggatcgt cacctcggtt gcagtccggc tcgggtagag atcggtgcgt
     4981 tccgacgcga cagtcatgac tcagtcctcc tcggtcagca gcgggtagac gccgttctcg
     5041 tcgtggtcct cccgtccggt cacgggaggg ttgaagacgc agacgcagcg gaaatccgtc
     5101 ttcggcctca gggtgtgctt ttcgtggccg ttcagcaagt acatcgtgcc gggctcgatc
     5161 cagtgggtct cgcccgtctc gcggttggtc agctcggcct caccctcgac gcagagcacg
     5221 gcctcgatgt ggttggcgta ccacatgtcg gtctcggtgc ccgcgtagag gatggtctcg
     5281 tgcagcgaga aaccgacctt ctccttggcg agcacgatgc gcttgctctc ccaggtgccg
     5341 gacgccgact tgacgtgccg gtcggtgttc tcgatgtcct tgaacgatcg gacgatcaca
     5401 gtgtggtcgt gccctttctt ccgtcgttgt gtgtcaggcc gtctctcgga cggcgcgggc
     5461 gaggatgcgc agaccctcgt ccagttcgtc cgggctcacg gtgagcggcg gcagcagctt
     5521 gaccacctcg ctctccggcc cggaagtctc gaccagcaga cccagctcga aggcgcgctt
     5581 ggagacggcg cccgcgcggg acttgtcggc gaactccatg ccccagacca ggccccggcc
     5641 gcggacggag acgatggcct cgccgccgtg ctcgtcgagg atggcggtga gcgcctgctc
     5701 gacctgctca ccccgggcga gggtctgctt ctccatctgg ccgtcgctcc agtacgcgtc
     5761 cagcgcggcg gcggcggtga cgaaggccgg gttgttgccc cggaaggtgc cgttgtgctc
     5821 gcccggctcc cagatgtcca gctccggctt gaacaggcag agcgacatgg gcaggccgta
     5881 gccgccgatg gacttcgaca gggtgacgat gtccggggtg atgcccgcct cctcgaagga
     5941 gaagaacggg ccggtgcggc cgcagcccat ctggatgtcg tcgacgatca gcagcatgtc
     6001 gcggcgcttg cacaggtcgg cgagggcgcg cagccactcg gggcgggcca cgttgatgcc
     6061 gccctcgccc tggatggtct cgacgatgac cgcggccggg gtgttgaggc ccgagccctg
     6121 gtcctccagc agccgctcga accagaggaa gtcctgcatg gtgccgtcga agtagttgtc
     6181 gaacggcatc ggcgtgccgt gcaccagcgg gatgccggcc ccggcgcgct tgaaggcgtt
     6241 gccggtcacg gcgagcgagc ccagcgacat gccgtggaag gcgttggtga aggagacgat
     6301 ggactcgcgg cccttgacct tgcgggccag cttcagcgcc gactcgacgg cgttggtgcc
     6361 cgtcgggccg gggaacatga ccttgtacgg caggtcacgc gggcgcagca gcacgttctg
     6421 gaaggtctcc aggaacgcgc gcttggcggt ggtgcccatg tccaggccgt gggtgatgcc
     6481 gtcgcgctcg atgtagtcga tcagcgcgcg tttcagcacc gggttgttgt ggccgtagtt
     6541 gagggcgccg gcgccggcga agaagtcgag gtaggtgtgg ccgtcctcgt cgtgcaggta
     6601 gctgccctgg gcccggtcga agacggcggg ccagctgcgg cagtagctcc gtacctccga
     6661 ctcaagggtc tcgaagacgc tcagggcggg cggggtgatg gtcacgactg ctctcctggg
     6721 agatgggggt gaagcgggcg gaagtcggtg atacgcgcgg aagggcggcc gggggcttgc
     6781 ttcccggccg gaccttgagt tgctgtgcgg gcgttccggt gtcagtcggt gaacggtccg
     6841 atgcggtaca gcacctcggg gtcgtgcccc ccgtcgggga agagcccggc accgaagagg
     6901 acctcgcgct ccagctcggc gccgtggcgc ttggcgtaag aggtgaagag gcgctcggag
     6961 ggcgtgttgc ccggggtgat ggtggtctcg attccgtcca gccgcccctt ctcacggagc
     7021 cggtcggtga gcccgtccag cagggcggcg gcgaggccgc gtccgcgctg cgaggcgtcc
     7081 acggcgacct gccagacgac gagggtgtgc gggcgctcgg gccggaagta cccggtcacg
     7141 aacccgaccg gctcgccggc ggcgtcgcgt gccaccacgg aggtgccggc gaagtcgcga
     7201 caccacagga ggtagctgta ggaggagttg aggtcgaggg tgcgcgagtc acgggcgata
     7261 cgccagatgg ctgccccgtc ctcgatgccc gggtggtcca ggcgcaaatc ctctggcatt
     7321 tccttcatgg tggatcggcc acgttcaagg tcggcttgtg cggcggtcat gcaaattgaa
     7381 tttaccgatt cactctgaaa aatgcatcgc gggtcggggt tacatgacag cactttctgt
     7441 gttatcgggc gcgggggtgc gcgtgcgcga acgaccgccc gaagatggtg atttgtcctg
     7501 catataagcc acgaaacggt caccgtgtgg ggtcagtcac aagtgcgtaa cgcacgcgtg
     7561 acgcgtccga attacgcggt ttcggtgtcc ggaaaatcga tgcgtttagc ttcggggaga
     7621 gcgggcagac gaatagggga aggtgagtgg gaaagaattc ggaattccct gtaataggtc
     7681 taccgataag aatatggttc gctgtccgcg ccgaaggcgg tgcggatttc ggtgtgcccg
     7741 ggaacggaaa agcgcgacgg gccgccccgc gctcggcgtg agccgcgcac cggacggccc
     7801 gccggggccg gtggggacgg agatcagccc gcggggcgct cccaggccgc gatggccgcg
     7861 cgccgcgccg cttccgggtc gaccgaccgc cccgtgaggt cggtcagcgc cgcccccagc
     7921 gcggagaggc aggagtggac ggtggagccg gccgcggccg tgccgtagtg gttgacccgg
     7981 atcatctcct tggccagcgc tccgccgccg gccaccagcg gcagcaccgg gtccgccgcc
     8041 agcgcccgcg ccaccagttc cgaggcgtcc acgccctccg gcgcccgcag cgtggtcgcc
     8101 accggcgccg cgtggcgcgc ctcgcggacg tacggcgcga aaccgccgcc gagcgccttc
     8161 acgccggccc gggtggccgc cgccgcccgg gtgtgccggc cgatcaccgt ctccagcccc
     8221 tcgtcctcga tccgctccac gcacgcctgg agcgccacca tctccagctg ggcgggggcg
     8281 tgcagcagcg cggtgcgccc ggcgtcgatc caccgctcct tccagtccag cagcgagagg
     8341 tacgagcggc gcggcgcctg cgggttgtcc gccatccgct gccaggcccg ctcgctcacc
     8401 gagaccgccg agaccccggc cgggccgccc atcgccttct gtgcgccgat cacacagagg
     8461 tccacgcccc aggcgtccgg gagcagcggc tcggcgccga ccgaggccac cgcgtccacc
     8521 atgaagagcg cgccgtgctc acgcaccacc cggccgatcc cggccaccgg gttggtgttg
     8581 ccggtcgccg cctcggcgtg caccagcgag acgaagccga tctccgggtg ctccgccagc
     8641 gcctcggcga cctggtcggc gctcaccgcc gagtcgtagg gcaccgccag gtcgaccacg
     8701 cgcgccccgc agtcgcgcag ccagtcaccg aaggtctgcc cgtacgggcc ggtgatcacg
     8761 ttgagggcgg tggtgtccgg gcccgccacg ccgcggatgc agccctccag cggcagcagc
     8821 gcctcaccct gagtgatgac gacgtcctgc gcggtggaga gcagcgccgc gacgcggcgc
     8881 tcgatgttcg cgaaatgcgc ggcggtcagc ggggtcaggt ccaggagggg gtgcgtcacg
     8941 gtggtgctct cttcgccggt cgatcgtcgg gctgccggtc cgagcgtacc caccgggtcc
     9001 tacgctgccg tgcatgagcg aagacatggt gctgcacgtg aagggccgga tactggtcgg
     9061 tcccgaggag gccagggacg aactctgggt ggtgggcggg cggatcacct tcagccggcc
     9121 ggcgggcgcc cgcgaggtgc ggaccgtcga ggggtgggca ctgccgggcc tggtcgacgc
     9181 ccactgccat gtcggcctgg accggcacgg cccggtcgcc gccgacgtcg ccgagaggca
     9241 ggcgctcacc gaccgcgacg ccggggcgct gctgctgcgc gacgccgggt cgcccagcga
     9301 cacccgctgg atggacgaac gcgacgacct gccccgcgtc atccgggccg gccgccacat
     9361 cgcgcgcacc cgccgctaca tccgcaacta cgcccacgag atcgagccgg accaactcgt
     9421 cgcctacgtc ggccaggagg cgcggcgcgg ggacggctgg gtcaagctgg tcggcgactg
     9481 gatcgaccgg ggcgcgggcg acctgacgcc gtgctggccc cgcgaggcgg tcgaggccgc
     9541 catcgccgag gcgcaccggc tcggcgcccg ggtcaccgcc cactgcttcg ccgaggactc
     9601 gctgcgcgac ctggtcgagg cgggcatcga ctgcgtcgag cacgccaccg ggctcaccga
     9661 ggacaccatc ccgctcttcg ccgaacgtgg cgtcgccatc gtccccaccc tggtcaacat
     9721 cgccaccttc cccgacctcg cggcgggcgg cgaggcgaag ttccccgact ggtcggccca
     9781 catgcggcgg ctgtacgagc gccggtacga cacggtccgt gccgcctggg acgcgggcat
     9841 cccggtcttc gtcggcaccg acgcgggcgg cagcctcccg cacggcctgg tcgccgccga
     9901 ggtcgccgaa ctggtcaccg ccggcatccc gccgctgcag gcgctctccg ccacggcgtg
     9961 gggcgcccgc gactggctcg gccgccccgg actgaccgag ggggcgcccg gcgacctggt
    10021 ggtctacgcg agcgacccgc gcgccgacgt ccgggtgctg gcggcgcccc gccgggtggt
    10081 ggtcggcggc aaggtcgtcg cctgaccgcg agaagggtgt tgacgggccg ttacgggggc
    10141 cggagcagcg tcgttgtgct ccggccccgc cgcgtgtccg cgcccgcgtg agccgtgcgc
    10201 acgcccctcg ggggcccgga aacggcggtc aggggtgacg tgggggaacg gccgtgccga
    10261 tcaggtcgaa cgtctgcacc aaaaccaccc tttggagtga agtcgctccg ggttgtcacc
    10321 ccttcacccg cagtgcgtaa agattccggc aacgacgtcg tcggcggccg cctgtcccca
    10381 ggtgcggccc cggcggctc
//
