LOCUS       NZ_CP106650            10216 bp    DNA     linear   CON 16-MAY-2024
DEFINITION  Streptomyces sp. GBA 94-10 4N24 strain GBA 94-10 chromosome,
            complete genome.
ACCESSION   NZ_CP106650
VERSION     NZ_CP106650.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. GBA 94-10 4N24
  ORGANISM  Streptomyces sp. GBA 94-10 4N24
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7025021)
  AUTHORS   Ian,E., Malko,D.B., Sekurova,O.N., Bredholt,H., Ruckert,C.,
            Borisova,M.E., Albersmeier,A., Kalinowski,J., Gelfand,M.S. and
            Zotchev,S.B.
  TITLE     Genomics of sponge-associated Streptomyces spp. closely related to
            Streptomyces albus J1074: insights into marine adaptation and
            secondary metabolite biosynthesis potential
  JOURNAL   PLoS One 9 (5), e96719 (2014)
   PUBMED   24819608
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025021)
  AUTHORS   Ruckert,C., Busche,T. and Kalinowski,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2022) CeBiTec, Uni Bielefeld, Sequenz 1, Bielefeld
            33615, Germany
REFERENCE   3  (bases 1 to 7025021)
  AUTHORS   Ian,E., Sekurova,O.N., Malko,D.B., Ruckert,C., Borisova,M.E.,
            Albersmeier,A., Kalinowski,J., Gelfand,M.S. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2013) CeBiTec, Bielefeld University,
            Universitaetsstr. 27, Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-NOV-2021
            Assembly Method                   :: flye v. 2.9-b1768
            Assembly Name                     :: GB94_v2
            Genome Coverage                   :: 229.4x
            Sequencing Technology :: Illumina MiSeq;Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000495635.2-RS_2024_05_16
            Annotation Date                   :: 05/16/2024 06:31:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,298
            CDSs (total)                      :: 6,204
            Genes (coding)                    :: 5,988
            CDSs (with protein)               :: 5,988
            Genes (RNA)                       :: 94
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 100 of 216
            Pseudo Genes (incomplete)         :: 164 of 216
            Pseudo Genes (internal stop)      :: 24 of 216
            Pseudo Genes (multiple problems)  :: 67 of 216
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:13:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 701709
            Orig. end                         :: 711925
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP106650.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Wittmann Lab, Saarland
            University, Germany.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5216
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(562..571)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            685..1590
                     /locus_tag="B591_RS02780"
                     /old_locus_tag="B591_02784"
     CDS             685..1590
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412452.1"
                     /locus_tag="B591_RS02780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_02784"
                     /product="hypothetical protein"
                     /protein_id="WP_023416052.1"
                     /transl_table=11
                     /translation="MRSALRSSRSVPSRRRRGRGRVAVGATLAVAALFTAAPPALADTS
                     PSPSASQDEEAEAGPTEAGTAFRTAARLAQGQRGTASASTGDYLYWVFPADTGQRPTVR
                     ATVSLPAEQARQGASTWRIDVYDGLRRRQPCTYGLQERRAAEDAAEVRLTCTLRTVRAF
                     SEPWANDPLPGAYYVRLTVSRLAADDLGRPFQAEVAVDSQETGGSTAVGGRLSEPLTPG
                     ISAVSGAEQTEEETAEEDGAEQAPRPAALAEPEDGWSSGWWTDRWLWTAGGAVLAALAG
                     IFGYTLTRGGGRPGRVPPGV"
     gene            1781..3598
                     /locus_tag="B591_RS02785"
                     /old_locus_tag="B591_02789"
     CDS             1781..3598
                     /NRPS_PKS="Domain: AMP-binding (22-474). E-value: 1.3e-86.
                     Score: 282.7. Matches aSDomain:
                     nrpspksdomains_B591_RS02785_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412454.1"
                     /locus_tag="B591_RS02785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_02789"
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="WP_037786916.1"
                     /sec_met_domain="AMP-binding (E-value: 7.3e-81, bitscore:
                     270.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREVSVGRLVAPPVSGGLADSVFHTAATHPERPQLALEPTTPGGP
                     WRTVTAARLRDEVLALARGLLAEGVRPGDRVVLMSRTRPEWTLLAYALWTLGAEVVPVY
                     PTSSIEQVRRVLADAGVRAAVVEHGGQAMTIAAACADPGALLGLWQLDLDCTAELAERG
                     GEVPEDEVLRLRAAVRPEATAVICYTSGTTGAARGCVISHANLAAECDTLIAGWGHLLA
                     PRGEQPSVLAFLPLAHCYGLMVAVTCVRGGYLLAHQPEMAPAVLLPALASFRPTSLFAV
                     PYIFERIFDEACREAEAAGHAGLFEKARVAAVAHAEAQEKRTAGTGHGPGPVTRFRHSV
                     YERAVYAKVRAVFGGRVRSAVSGGSPLRRELGLFFQGAGVTVYDGYGLTETTAAVTAQP
                     PGRVRFGTVGRPLPGSTVRIAGDGEVWVRGDTVFRGYLHDPPGLADGWLATGDTGRFDP
                     DGYLVITGRKKDIIITSGGKSVSPQLLEEQLRRHPLIAQCLVVGDNRPYVAALLTVDQE
                     ALAHWQRLRGASGRAASAPADGAELRAQLQRAVNQANTLVSRAESIRAFEILPVDFTRQ
                     EGLMTPSFKLRRSAVADTYAEVIEALYAR"
     aSDomain        1847..3202
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B591_RS02785_AMP-binding.1"
                     /evalue="1.30E-86"
                     /label="B591_RS02785_AMP-binding.1"
                     /locus_tag="B591_RS02785"
                     /protein_end="474"
                     /protein_start="22"
                     /score="282.7"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FHTAATHPERPQLALEPTTPGGPWRTVTAARLRDEVLALARGLLA
                     EGVRPGDRVVLMSRTRPEWTLLAYALWTLGAEVVPVYPTSSIEQVRRVLADAGVRAAVV
                     EHGGQAMTIAAACADPGALLGLWQLDLDCTAELAERGGEVPEDEVLRLRAAVRPEATAV
                     ICYTSGTTGAARGCVISHANLAAECDTLIAGWGHLLAPRGEQPSVLAFLPLAHCYGLMV
                     AVTCVRGGYLLAHQPEMAPAVLLPALASFRPTSLFAVPYIFERIFDEACREAEAAGHAG
                     LFEKARVAAVAHAEAQEKRTAGTGHGPGPVTRFRHSVYERAVYAKVRAVFGGRVRSAVS
                     GGSPLRRELGLFFQGAGVTVYDGYGLTETTAAVTAQPPGRVRFGTVGRPLPGSTVRIAG
                     DGEVWVRGDTVFRGYLHDPPGLADGWLATGDTGRFDPDGYLVITGRKKDIIIT"
     CDS_motif       2330..2389
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B591_RS02785_0001"
                     /evalue="7.00E-06"
                     /label="NRPS-A_a3"
                     /locus_tag="B591_RS02785"
                     /protein_end="203"
                     /protein_start="183"
                     /score="18.7"
                     /tool="antismash"
                     /translation="AVICYTSGTTGAARGCVISH"
     CDS_motif       3047..3124
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B591_RS02785_0002"
                     /evalue="8.10E-06"
                     /label="NRPS-A_a6"
                     /locus_tag="B591_RS02785"
                     /protein_end="448"
                     /protein_start="422"
                     /score="18.3"
                     /tool="antismash"
                     /translation="GEVWVRGDTVFRGYLHDPPGLADGWL"
     gene            3821..4327
                     /locus_tag="B591_RS02790"
                     /old_locus_tag="B591_02794"
     CDS             3821..4327
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640757.1"
                     /locus_tag="B591_RS02790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_02794"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_023416054.1"
                     /transl_table=11
                     /translation="MRIRAVHQTELTALQDIERAAGVWFREIGMAEIAEDEPPSVAELS
                     RYARSGLAWVAAGGEGRPVAYLIADRVDGNLHVEQVTVHPDSARRGVGRALLDHTAGRA
                     RDEGAAALTLTTFTEVPWNAPYYERCGFRVLAERELTPGLREIRRAEAAHGLDRWPRCC
                     MRREV"
     misc_feature    complement(4515..4532)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.07"
                     /tool="antismash"
     misc_feature    4950..4970
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.98"
                     /tool="antismash"
     gene            5001..5216
                     /locus_tag="B591_RS02795"
                     /old_locus_tag="B591_02799"
     CDS             5001..5216
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="B591_RS02795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_02799"
                     /product="hypothetical protein"
                     /protein_id="WP_008412458.1"
                     /sec_met_domain="strepbact (E-value: 4.2e-37, bitscore:
                     125.0, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYTPQVETAEIADSELDNISGGLVNGLVGSVTSSLDSVAPVSDT
                     LGTVTGVVEQTTGLNTAPVTGLVAGL"
     misc_feature    5015..5030
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.97"
                     /tool="antismash"
     gene            5414..6220
                     /locus_tag="B591_RS02800"
                     /old_locus_tag="B591_02804"
     CDS             5414..6220
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640761.1"
                     /locus_tag="B591_RS02800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_02804"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_023416055.1"
                     /transl_table=11
                     /translation="MRFRQQALTKLQSPEQLDLPVRFARPQGLLVLAVTLVVMAAGGVW
                     AVTGSVDTTLTAPGVLTHAQGSYVVQSTVSGQVTAVRTAEGRRVARGTPLLTVRTARGD
                     VTIRALAAGRVTALAAATGAVVTTGTDLAAVERQKNPDDPLLAVLYAPADSGASLRPGA
                     KADLTVQSVPAQSYGVLRGTVKSVGRTARTRQQISADLGSAQLGEEFSRNGPPIAVEVR
                     LHPAKSTRSGYTWSTSDGPPHPLTTLTLVDGAVHLDEQRPADWLLP"
     gene            6217..8514
                     /locus_tag="B591_RS02805"
                     /old_locus_tag="B591_02809"
     CDS             6217..8514
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640763.1"
                     /locus_tag="B591_RS02805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B591_02809"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_023416056.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.1e-36, bitscore:
                     121.9, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPADAPAAGAGSTAPPTPGGRRGRRAAPSSGRRRAAPPRRTPR
                     RRPVRTPTVLQMEAVECGAACLAMVLAHHGRHVPLEELRIACGVSRDGSRASNVLKAAR
                     SYGLTAKGMQMEPAALREVQAPAVLFWEFNHYVVHEGFLRRPGRRGAVRLNDPDKGRRV
                     VSAEEFDASFTGVALVLEPGPSFRKGGRKPGLLGALPARLRGTGATLLTALLASLLLVV
                     VGAALPALSRTYIDLFLIGERTSVLGPLFVAMGALVALTASLTALQQANLLRGRIISST
                     LSSARFLRHLLRLPVTFFAQRSPADLVQRLQSNDTVAETLARDLAAAAVDGVVVVLYAL
                     LLWTYDPQLTLVGVGIALLNIVAMRVVIQLRATRTQKLRADNARLTTTAYTGLQLIETM
                     KATGGEDGFFRRWAGQHATTLEEQQRLGVPTAVLSVVAPALATFNSALILWIGGLRAVE
                     GHLSVGLLVAFQALMVRFTAPLTRLNGVAGRIQDFAADVTRLRDVEAFPADGLYRREGG
                     AGGEADTRRLAGRVELEGLTFGYSPLDPPLLTDFSLTVGPGRQVALVGGSGSGKSTVSR
                     LLAGLYQPWEGTIRIDGQPLTEVPRSALAASVSFVDQDVFLFEGTVRENVTLWDPSLPD
                     EAVRTALRDAGLEEVIARRPDGIHARVEQDGRNFSGGQRQRLEIARALVRRPSVLVLDE
                     VTSALDAETEQRVMAALRRRGCACVIIAHRLSTVRDSDEIVVLDGGRVVERGRHAELVA
                     RGGAYAALVEEK"
     misc_feature    complement(8564..8576)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
ORIGIN
        1 catcgcggag gcgaccggcg gcacctacac cgccgtgcag cacaaggagg acctctccga
       61 caaggtgaag cagctggtgg accgcgcggc cgacccggtg gtcacgccgg tgccggtcga
      121 gggcgccgcg gcctgtaccg acgcgccgaa gatcgagccg ggcctgtaca ccgaccgcca
      181 gccgctcggc acccaccgct ggtaccgggt ggacgtgctg cccggccagg agctgcgcgc
      241 ctcggtgagt gtggcggccg accggccgct cgccgacgac tacggcgtgc tgctgcgcgc
      301 ggtgaccgcg cacggccgcg agctggtacg cggctcggag gcgggcagcg gccggaccga
      361 cgtgatctcg tccggcctgc gccacccgga gcgggagcgg gaggacgccg acaccgacaa
      421 gccggccgcg accccggtct gcctggaggt cagcaactcc ttcgcccccg cctccgccaa
      481 ggccgccgac gcctcggcac cgggcatgcc gctggagctg acggtcgacg tggtcgacgg
      541 accgtccgac gcgtcggaca cggccgcctt cgggctgggc cgcggctggt ggctgctggg
      601 cgtgctggtg ctcgccgggt tcctcggcgg tctgatcttc ggctggctgt cgcgctggcg
      661 cttcgccgtc tggaggaccc gctgatgcgt tccgctcttc gcagctcccg gtccgtgccg
      721 tcccggcgcc ggcgcgggcg cgggcgcgtc gccgtggggg ccaccctcgc cgtggcggcc
      781 ctgttcaccg ccgcgccgcc ggccctcgcc gacacctcgc cctcgccctc cgcctcgcag
      841 gacgaggagg ccgaggccgg tccgaccgag gcgggcaccg cgttccgcac ggcggcgcgg
      901 ctcgcgcagg ggcagcgcgg caccgcctcc gcgtcgaccg gtgactacct ctactgggtc
      961 ttcccggccg acaccgggca gcgcccgacg gtacgggcga ccgtctcgct ccccgccgaa
     1021 caggcccggc agggcgcctc cacctggcgg atcgacgtct acgacgggct ccgccgccgc
     1081 cagccgtgca cctacggcct ccaggagcgg cgggccgccg aggacgccgc cgaggtgcgg
     1141 ctgacctgca cgctgcgtac cgtgcgggcc ttctccgagc cgtgggccaa cgacccgctg
     1201 cccggggcgt actacgtccg gctgacggtc tcgcggctcg ccgccgacga cctgggccgg
     1261 ccgttccagg ccgaggtggc ggtcgactcc caggagaccg gcggctccac ggcggtcggc
     1321 gggaggctct ccgaaccgct gacgccgggc atctcggcgg tctccggcgc ggagcagacc
     1381 gaggaggaga cggccgagga ggacggtgcc gagcaggcgc cgcgtccggc ggcgctcgcc
     1441 gagccggagg acggctggtc ctccggctgg tggaccgacc gctggctgtg gacggccggg
     1501 ggtgccgtac tggccgccct cgcgggcatc ttcggctaca ccctgacccg gggcggcggc
     1561 cgccccggcc gggtgccgcc cggcgtctga cgggccgacg gctccacgcg tccccggccg
     1621 ggcggcgcgc ggagccgccc ccggcgcacg gtggtgaact cccccgtcac ccctggccag
     1681 ggcgtgcggg gcgctgtagg ttgccgagcc ggccatccgt caggtacggg ccccgcactg
     1741 gtgcggcacg cggccgggat gacgaacgga ggcgtacccg gtgcgggaag tgagtgtggg
     1801 gcgcctggtc gccccgccgg tctccggcgg cctcgccgac tcggtcttcc acacggccgc
     1861 gacgcacccc gaacgccccc aactggccct ggaacccacc acgccgggcg gcccctggcg
     1921 caccgtcacg gcggcccggc tccgggacga ggtgctggcg ctggcccggg gcctgctggc
     1981 cgagggcgtg cggcccggcg accgggtggt gctgatgtcc cgcacccgcc ccgagtggac
     2041 gctgctggcg tacgccctgt ggacgctggg cgccgaggtg gtaccggtct accccacctc
     2101 ctccatcgag caggtccgcc gggtcctcgc cgacgcgggt gtccgggcgg ccgtggtgga
     2161 acacggcggg caggcgatga cgatcgcggc ggcctgcgcc gaccccggcg ccctgctcgg
     2221 actctggcag ctggacctgg actgcacggc cgaactcgcc gagcgcggcg gggaggtgcc
     2281 ggaggacgag gtgctgcggc tgcgggcggc ggtccgcccg gaggcgaccg ccgtcatctg
     2341 ctacacctcg ggcaccaccg gcgcggccag gggctgtgtg atcagccacg ccaacctggc
     2401 cgccgagtgc gacaccctca tcgcgggctg gggccacctg ctggcccccc gcggcgaaca
     2461 gccctcggtc ctcgccttcc tgccgctggc ccactgctac gggctgatgg tggcggtcac
     2521 ctgcgtacgc ggcggctatc tcctcgccca ccagccggag atggcccccg cggtgctcct
     2581 cccggccctc gcctcgttcc ggcccacctc cctcttcgcc gtgccctaca tcttcgagcg
     2641 gatcttcgac gaggcgtgcc gggaggccga ggccgccggg cacgcgggac tcttcgagaa
     2701 ggcacgggtg gccgccgtgg cccacgccga ggcccaggag aagcgcacgg cgggcaccgg
     2761 acacggcccg gggccggtga ccaggttccg gcactccgtc tacgagcggg ccgtctacgc
     2821 caaggtccgg gcggtcttcg gcggacgggt ccgctccgcc gtctcgggcg gctcgcccct
     2881 ccgccgcgaa ctgggactgt tcttccaggg cgcgggggtg accgtctacg acggttacgg
     2941 gctgaccgag accacggcgg cggtgaccgc gcagccgccg ggccgggtcc gcttcggcac
     3001 cgtcgggcgc ccgctgccgg ggtcgaccgt ccggatcgcc ggcgacggcg aggtgtgggt
     3061 acgcggggac accgtcttcc ggggctatct gcacgacccg ccggggctcg ccgacggctg
     3121 gctggccacc ggggacaccg gccgcttcga cccggacggc tatctggtga tcaccggccg
     3181 caagaaggac atcatcatca ccagcggcgg aaagagcgtc tccccgcagt tgctggagga
     3241 gcagttgcgc cgccacccgc tgatcgccca gtgcctggtg gtaggcgaca accggcccta
     3301 cgtggcggcc ctgctcaccg tcgaccagga ggccctggcc cactggcagc gcctgcgcgg
     3361 cgcctccggc cgggccgcct cggcaccggc cgacggcgcg gagctacggg cccagctcca
     3421 gcgggcggtc aaccaggcca acaccctggt ctcgcgggcc gagtcgatcc gggccttcga
     3481 gatcctcccg gtcgacttca cccgccagga gggcctgatg accccctcgt tcaaactccg
     3541 ccgcagcgcc gtcgcggaca cgtacgcgga ggtcatcgag gcgctgtacg cacgctgagc
     3601 ggcggtgcgg ggcgggccgc gaggaccccc gcgcaaagaa atccccgccc caggtggcat
     3661 gaccgccccc actccgggca cacgcccctc agaccgacct gacggccctt ccccctggcc
     3721 tggcaggttg cctcggcctc accggacttc ccccccttcg tccggtgggg ccgccccttt
     3781 ttccgttgct ccccgccccc gcgcaccgga ccatgccccc atgcgtatcc gagcggtgca
     3841 ccaaaccgaa ctgacggctc tccaggacat cgagcgggcc gccggagtgt ggttccggga
     3901 gatcggcatg gccgagatcg cggaggacga gccaccttcg gtggcggagc tgtcgcggta
     3961 cgcgcggtcg gggctcgcct gggtggcggc cggcggggag ggccggcccg tggcgtacct
     4021 gatcgcggac cgggtcgacg ggaacctcca cgtcgagcag gtgacggtcc atccggacag
     4081 cgcgcgccgg ggtgtcggcc gggccctgct ggatcatacg gccgggcggg cccgggacga
     4141 gggggctgcg gcgctgacgc tcaccacctt caccgaggtg ccgtggaacg cgccgtacta
     4201 cgagcggtgc ggcttccgcg ttctggcgga gcgtgagctg acgccggggc tgcgggagat
     4261 ccggcgggcc gaggcggcgc acggactgga ccggtggcct cgctgctgca tgcgccggga
     4321 ggtgtgagca gagccgtcca gggagacagc tggtccgccg ccggggaatg cccgccgccg
     4381 accgcccttt cgcggccttg ccggtaattc cgctcccgcc tcgccgtccg cgtttcgcgg
     4441 tgcgcaagaa ttcgccgccc gacggcaatt cccccgcttc cgccccttct tcccctcgct
     4501 gcggactctg ttcctccaca tgttgggaac atggccttct cagggccgtc cgcacacgaa
     4561 tccgccgcgc cccggaacgg gttctcctgc ggtgaatctg cacagccgtg ccgcagctcc
     4621 gtcaccgcca cccaaacctt caccgcgtgt ggacggccga ggtcggcggc ccgcaccagg
     4681 gcttcacagc aaggggtgtg gtgtacaccc gagcgtgacc ccaccccgta cggcggcggg
     4741 aaggtgtgaa ggtgatgccg ttctcctgtg cccgatcccg cacacggtgg tgaggagaac
     4801 ggcaccgccc gccgtattcc gctctcgtcg attggccgcc ccgcgcaccg tcgtgctttg
     4861 atccatcgca ctgcggcgcc gcccccggaa ctgaagggtc atcgcctttc gcccgggggc
     4921 cgccagaaga tttccccttc ttctgcacga gtgcgtcgcg gtggctattt ccgtcagtga
     4981 tgcgaaggga actccccccc atgagctaca ccccccaggt cgagaccgca gagatcgccg
     5041 acagcgagct ggacaacatc tccggcggcc tcgtgaacgg cctcgtcggc tcggtcacct
     5101 ccagcctcga ctccgtcgcc ccggtctccg acaccctcgg caccgtcacc ggcgtcgtgg
     5161 agcagaccac cggtctgaac accgccccgg tcaccggcct cgtcgccggt ctctgagacc
     5221 ccacgcgtcg cctgacgacg cgtgcggccc gctgaggcgg accgctcggc cggtgccccg
     5281 gacccgctcg ggttccgggg caccggccgc ctccttgtcc tcccccggac gcccgcgcgg
     5341 cgtccccagc accacggcga cacccggccg cacggccgaa gaggccggtg cccggcagcc
     5401 gaggaagtct tccgtgcgct ttcgccaaca ggccctgacc aagctccagt cgcccgaaca
     5461 gctggacctg ccggtgcggt tcgcccgccc gcagggactc ctggtcctcg ccgtcaccct
     5521 cgtcgtgatg gcggccggcg gcgtgtgggc cgtcaccggt tcggtcgaca ccaccctcac
     5581 cgcgccgggc gtcctcaccc acgcccaggg cagctacgtc gtccagtcca ccgtctccgg
     5641 ccaggtcacc gccgtccgca ccgccgaggg ccgccgcgtc gcccgcggca ccccgctgct
     5701 gaccgtccgc accgcgcggg gcgacgtgac catccgcgcc ctggccgccg ggcgggtcac
     5761 cgcgctggcc gccgccaccg gcgccgtcgt caccaccggc accgacctgg ctgccgtcga
     5821 gcgccagaag aaccctgacg acccgctcct cgcggtgctc tacgcccccg ccgacagcgg
     5881 cgcgtccctg cggcccggcg ccaaggccga cctcaccgtg cagtcggtgc cggcccagag
     5941 ctacggcgtg ctgcgcggca ccgtgaagtc ggtcggccgc accgcccgga cccggcagca
     6001 gatctccgcc gacctcggct ccgcccagct cggcgaggag ttctcccgga acgggccgcc
     6061 gatcgccgtc gaggtccgcc tgcaccccgc gaagagcacc cgcagcggtt acacctggtc
     6121 gacctccgac ggcccgccgc acccgctcac caccctcacc ctcgtcgacg gcgccgtcca
     6181 cctggacgaa cagcgcccgg ccgattggct cctcccgtga ccgcacccgc cgacgccccc
     6241 gccgccgggg ccggctccac cgccccgccc accccgggcg ggcgccgggg ccgccgcgcc
     6301 gccccctcct ccggccgccg ccgcgccgcg ccgccgcgcc gtacgccccg gcggcggccc
     6361 gtccgcaccc ccaccgtcct gcagatggag gcggtggagt gcggcgccgc ctgcctcgcc
     6421 atggtcctcg cccaccacgg gcggcacgtc ccgctggagg agctgcggat cgcctgcggc
     6481 gtctcccgcg acggctcgcg cgccagtaac gtcctcaaag ccgcccgcag ttacggcctg
     6541 accgccaagg gcatgcagat ggaaccggcc gccctgcgcg aggtgcaggc ccccgccgtg
     6601 ctgttctggg agttcaacca ctacgtcgtc cacgaggggt tcctgcggcg tcccgggcgg
     6661 cgcggcgcgg tccgcctcaa cgaccccgac aaggggcgcc gggtggtgag cgccgaggag
     6721 ttcgacgcct cgttcaccgg cgtcgccctg gtcctcgagc cgggcccctc gttccgcaag
     6781 ggcggccgca aacccgggct gctcggcgcg ctcccggccc ggctgcgcgg caccggcgcc
     6841 accctgctga ccgcgctcct ggccagcctg ctgctggtcg tggtgggcgc ggcgctgccc
     6901 gcgctcagcc ggacgtacat cgacctgttc ctgatcgggg agcggacctc ggtgctcggg
     6961 ccgctcttcg tggcgatggg tgcgctggtg gcgctgaccg cctcgctgac cgcgctccag
     7021 caggccaatc tgctgcgcgg gcggatcatc tcctcgacgc tgagcagcgc ccggttcctg
     7081 cgccatctgc tgcggctgcc ggtgaccttc ttcgcccagc gctcccccgc cgacctggtg
     7141 cagcggctcc agtcgaacga cacggtggcc gagaccctcg cccgcgacct ggcggccgcc
     7201 gccgtcgacg gggtggtggt ggtgctgtac gcgctgctgc tgtggacgta cgacccgcaa
     7261 ctgaccctgg tcggggtggg gatcgcgctg ctgaacatcg tggcgatgcg ggtggtgatc
     7321 cagctgcggg ccacccggac gcagaagctg cgggccgaca acgcgcggct caccaccacc
     7381 gcgtacaccg ggctccagct catcgagacg atgaaggcga ccggcggcga ggacggcttc
     7441 ttccggcggt gggcggggca gcacgcgacg acgctggagg agcagcagcg gctcggggtg
     7501 ccgacggcgg tgctgtcggt ggtggccccg gccctcgcca ccttcaacag cgcgctgatc
     7561 ctgtggatcg gcggactgcg ggcggtcgag gggcatctgt cggtcgggct gctggtggcg
     7621 ttccaggcgc tgatggtgcg gttcacggcg ccgctgaccc ggctcaacgg cgtcgccggc
     7681 cgcatccagg acttcgcggc cgacgtgacc cggctgcggg acgtcgaggc gttcccggcg
     7741 gacgggctgt accgccgcga gggcggggcg ggcggcgagg cggacacccg gcggctggcg
     7801 ggacgggtgg agctggaggg gctgaccttc ggttacagcc cgctggaccc gccgctgctg
     7861 acggatttct cgctgacggt cggtccgggg cggcaggtgg cgctggtcgg cgggtcgggc
     7921 agcggcaagt cgacggtatc ccggctgctg gcgggcctgt accagccgtg ggagggcacc
     7981 atccgcatcg acgggcagcc gctcaccgag gtcccgcgct ccgcgctggc cgcctcggtc
     8041 tccttcgtcg accaggacgt cttcctcttc gagggcacgg tgcgggagaa cgtcaccctc
     8101 tgggacccct cgctgcccga cgaggcggtc cgcaccgcgc tgcgcgacgc cggactggag
     8161 gaggtgatcg cccgccgccc ggacggcatc cacgcccggg tcgagcagga cgggcggaac
     8221 ttctccggcg gccagcggca gcggctggag atcgcccggg ccttggtgcg ccgccccagc
     8281 gtgctggtgc tggacgaggt gaccagcgcc ctcgacgcgg agaccgagca gcgggtgatg
     8341 gcggcgctca ggcggcgcgg ctgcgcctgc gtgatcatcg cgcaccgcct ctccaccgtc
     8401 cgcgacagcg acgagatcgt ggtgctggac ggcggccggg tcgtggaacg cggacgccac
     8461 gccgaactgg tcgcccgggg cggggcgtac gcggccttgg tcgaggagaa gtgagatgac
     8521 gggcacccag caggctcccc accccatccc ggccgccgac ccggtgacgg cggcactcgg
     8581 cggactcggc gcaccggtgg cgggggccga cgggtcggcc ctgccgctcg gcgggccctc
     8641 ggcgctctgg ctggtcaccg agggcagcct cgacctcttc gcggtggcgg ccgccgagga
     8701 cggccactgg cactacctcg gccgcctgga ggccggcacg ctcctgctcg gcgccgtcga
     8761 gggcccccgc cacaccctgg tgggccgcct cacctcccac tgcgccgtac gccgcctgcc
     8821 gctgcgcgag ctgtacgcgc cgcagggcgc cgaggggtac gaggggtacc ccgggtacga
     8881 gccggtcgcc gggccgctgg agcacgcggt ggggctcggc ctcggccggg ggctcgccgt
     8941 gctgttcgcg gcaccccagg cggacgggcc cgcggcgggc tccgggcccg ccgacgagga
     9001 cgtgctgtgg gtggacgtcc cgccgggcag tgtccggctg ggggccgagt tcgccgccga
     9061 ggcggcggcc gacctgctgg tcggcggagc gctgtggcag tccctggtgg accagcagta
     9121 ccgcctgctg accgccgtcg acggctgggt ggagcggctg gagcgggccc acgaggaccg
     9181 ggcggcggcc ggtatccggg ccggtgaggc ggtacgggag cgggccgacc aggcgctgct
     9241 cgactcgatc ggccgccgcg accgctcccc ccgggcacgc ggcgaggcgg gcgacgacgc
     9301 ggtgtacgcg gtcttccgcc aggttgcgcg ggcgagcggc atcgcgctgc cggaggcggc
     9361 ggcgcgttcg gtcgcgggcc gcggacggct cgacccggtg gagcggatgg ccgccgccgc
     9421 ccggctgcgc acccgcccgg tgcggctggc cggcgagtgg tggcgcaccg acgccggacc
     9481 gctggtgggg cggcgcgcca agtccggcgc gccggtggcc ctgctctggc ggcgcggccg
     9541 gtacgaggcg ctcagcccgg cgaccggcgc acgtatccgg atcggcgcca aggaggcggc
     9601 cgagttcgag gaacgcgcca cgatgctcta ccggccgctg cccgaggggc cgctgacgct
     9661 gcgccggctg atgctcttca gcctgcgcgg ctcccggggc gacctgcgca acctcgtcct
     9721 gagcggcctg gtcaccgtgg cgctgggcgc gctggtgccg gtggcgaccg ggcaggtgct
     9781 cggcgcgtac gtgccgagcg cgcagaccgg cctgatcgcg cagatgtgcg tggcggtgat
     9841 ggtggccggg gtggtctcgg cggcgttcat gctgctccag tcgctgacgc tgctgcgcct
     9901 ggaggggcgg atcgaggcgg cgttgcagcc ggccgtctgg gaccggctgc tcaggctgcc
     9961 gacggccttc ttcgcggacc gttccaccgg tgagctggcc agtgccgcga tggggatcag
    10021 cgcgatccgc cgggtgctgt cggggatcgg gccgaccgcc gtgcagtcgg tgacggtggc
    10081 cggggtcaac ctggtgctgc tgctggtgca cagcgtgccg atggcgctgg cggcgatcgg
    10141 ggtgctgctg gtggtctcgg tggtctttct ggtgctgggg ctctggcagg tgaagtggca
    10201 gcggaagctg gtggag
//
