GCF_000466185.1 - 4 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_KE951478.1 (Actinomyces graevenitzii F0530)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	terpene-precursor	36,702	57,808

NZ_KE951483.1 (Actinomyces graevenitzii F0530)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 5.1	terpene-precursor	82,170	103,276

NZ_KE951497.1 (Actinomyces graevenitzii F0530)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 18.1	lanthipeptide-class-i	1	9,030

NZ_KE951506.1 (Actinomyces graevenitzii F0530)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 27.1	RRE-containing	48,653	68,940

Region	Type	From	To
Region 1.1	terpene-precursor	36,702	57,808
Region 5.1	terpene-precursor	82,170	103,276
Region 18.1	lanthipeptide-class-i	1	9,030
Region 27.1	RRE-containing	48,653	68,940

No secondary metabolite regions were found in these records:: NZ_KE951479.1 (Actinomyces graevenitzii F0530); NZ_KE951481.1 (Actinomyces graevenitzii F0530); NZ_KE951482.1 (Actinomyces graevenitzii F0530); NZ_KE951484.1 (Actinomyces graevenitzii F0530); NZ_KE951485.1 (Actinomyces graevenitzii F0530); NZ_KE951486.1 (Actinomyces graevenitzii F0530); NZ_KE951487.1 (Actinomyces graevenitzii F0530); NZ_KE951488.1 (Actinomyces graevenitzii F0530); NZ_KE951489.1 (Actinomyces graevenitzii F0530); NZ_KE951490.1 (Actinomyces graevenitzii F0530); NZ_KE951492.1 (Actinomyces graevenitzii F0530); NZ_KE951493.1 (Actinomyces graevenitzii F0530); NZ_KE951494.1 (Actinomyces graevenitzii F0530); NZ_KE951495.1 (Actinomyces graevenitzii F0530); NZ_KE951496.1 (Actinomyces graevenitzii F0530); NZ_KE951498.1 (Actinomyces graevenitzii F0530); NZ_KE951499.1 (Actinomyces graevenitzii F0530); NZ_KE951500.1 (Actinomyces graevenitzii F0530); NZ_KE951501.1 (Actinomyces graevenitzii F0530); NZ_KE951502.1 (Actinomyces graevenitzii F0530); NZ_KE951503.1 (Actinomyces graevenitzii F0530); NZ_KE951504.1 (Actinomyces graevenitzii F0530); NZ_KE951505.1 (Actinomyces graevenitzii F0530)

Compact view

NZ_KE951478.1 - Region 1 - terpene-precursor

Download region GenBank file

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Location: 36,702 - 57,808 nt. (total: 21,107 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase

HMPREF1978_RS00240: acyltransferase

smcogs: SMCOG1141: acyltransferase

Ligases: synthetase

HMPREF1978_RS00220: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002309.2	0.37	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001910.4	0.33	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000936.5	0.32	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0000664.5	0.32	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001227.3	0.31	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000678.5	0.31	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000649.3	0.30	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002294.3	0.30	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0000286.3	0.30	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0000637.3	0.29	terpene	carotenoid	Corynebacterium glutamicum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002309.2	0.37	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0001910.4	0.33	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000936.5	0.32	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0000664.5	0.32	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001227.3	0.31	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000678.5	0.31	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000649.3	0.30	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002294.3	0.30	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0000286.3	0.30	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0000637.3	0.29	terpene	carotenoid	Corynebacterium glutamicum

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

HMPREF1978_RS00220: PT_FPPS_like

Domain 1 (48 - 340): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 20.58 out of 23.05

Confidence: strong

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.79 out of 21.84

Confidence: medium

Additional weak hits (1):

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 22.00 out of 35.63

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
MexT	Global virulence regulator

NZ_KE951483.1 - Region 1 - terpene-precursor

Download region GenBank file

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Location: 82,170 - 103,276 nt. (total: 21,107 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthetase

HMPREF1978_RS01600: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000638.3	0.33	terpene	carotenoid	Pantoea ananatis
BGC0000678.5	0.32	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000643.3	0.32	terpene	carotenoid	Brevundimonas vesicularis
BGC0000634.3	0.32	terpene	carotenoid	Brevundimonas sp. SD212
BGC0001227.3	0.31	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000665.5	0.29	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000666.5	0.26	terpene	xiamycin A	Streptomyces sp. SCSIO 02999
BGC0001782.3	0.26	other (other)	spiroindimicin A, spiroindimicin B, spiroindimicin C, spiroindimicin D, indimicin A, indimicin B, indimicin C, indimicin D, indimicin E, lynamicin A, lynamicin D, lynamicin F, lynamicin G	Streptomyces sp. SCSIO 03032
BGC0000905.5	0.25	other (other)	heme D1	Heliobacillus mobilis
BGC0000906.3	0.25	other (other)	heme D1	Heliophilum fasciatum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000638.3	0.33	terpene	carotenoid	Pantoea ananatis
BGC0000678.5	0.32	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000643.3	0.32	terpene	carotenoid	Brevundimonas vesicularis
BGC0000634.3	0.32	terpene	carotenoid	Brevundimonas sp. SD212
BGC0001227.3	0.31	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000665.5	0.29	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000666.5	0.26	terpene	xiamycin A	Streptomyces sp. SCSIO 02999
BGC0001782.3	0.26	other (other)	spiroindimicin A, spiroindimicin B, spiroindimicin C, spiroindimicin D, indimicin A, indimicin B, indimicin C, indimicin D, indimicin E, lynamicin A, lynamicin D, lynamicin F, lynamicin G	Streptomyces sp. SCSIO 03032
BGC0000905.5	0.25	other (other)	heme D1	Heliobacillus mobilis
BGC0000906.3	0.25	other (other)	heme D1	Heliophilum fasciatum

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

HMPREF1978_RS01600: PT_FPPS_like

Domain 1 (38 - 323): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.59 out of 23.05

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

Additional weak hits (4):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.65 out of 21.55

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.29 out of 25.75

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.02 out of 32.93

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.61 out of 32.93

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
VqsM	Regulator of quorum-sensing signalling systems

NZ_KE951497.1 - Region 1 - lanthipeptide-class-i

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Location: 1 - 9,030 nt. (total: 9,030 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase dehydratase

HMPREF1978_RS05690: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

HMPREF1978_RS09400: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000541.5	0.47	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000556.5	0.46	ribosomal	streptin	Streptococcus pyogenes
BGC0002323.2	0.45	ribosomal	nisin J	Staphylococcus capitis
BGC0000506.4	0.45	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.44	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000539.5	0.44	ribosomal	nukacin ISK-1	Staphylococcus warneri
BGC0001210.5	0.44	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000626.3	0.44	ribosomal	thuricin	Bacillus thuringiensis
BGC0000536.3	0.43	ribosomal	nisin Q	Lactococcus lactis
BGC0000535.4	0.43	ribosomal	nisin A	Lactococcus lactis subsp. lactis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000541.5	0.47	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000556.5	0.46	ribosomal	streptin	Streptococcus pyogenes
BGC0002323.2	0.45	ribosomal	nisin J	Staphylococcus capitis
BGC0000506.4	0.45	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.44	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000539.5	0.44	ribosomal	nukacin ISK-1	Staphylococcus warneri
BGC0001210.5	0.44	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000626.3	0.44	ribosomal	thuricin	Bacillus thuringiensis
BGC0000536.3	0.43	ribosomal	nisin Q	Lactococcus lactis
BGC0000535.4	0.43	ribosomal	nisin A	Lactococcus lactis subsp. lactis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for HMPREF1978_RS05690

HMPREF1978_RS10280

Leader:

MATFDLDTRVEEAGETVRPQ

Core:

ITSKFLCTPGCPTGGIACFTTQCTNGCSISVACK

Core with dehydrations:

IDhbDhaKFLCysDhbPGCysPDhbGGIACysFDhbDhbQCysDhbNGCysDhaIDhaVACysK

antiSMASH-DB 4.0 matches for HMPREF1978_RS10280

100.0%

NZ_KE951497.1:

Lanthipeptides: allorf_8519_8683_lanthipeptide

ITSKFLCTPGCPTGGIACFTTQCTNGCSISVACK

67.6%

NZ_JALGRO010000002.1:

Lanthipeptides: MT346_RS01980_lanthipeptide

ITSKFLCTPGCPTGGIACFTTQCTNGCSIS

ITS LCTPGCPTGG ACFT CT GC+IS

ITSFSLCTPGCPTGGFACFTAACTGGCTIS

67.6%

NZ_SNVW01000005.1:

Lanthipeptides: EDF64_RS09155_lanthipeptide

ITSKFLCTPGCPTGGIACFTTQCTNGCSIS

ITS LCTPGCPTGG ACFT CT GC+IS

ITSVSLCTPGCPTGGFACFTAACTGGCTIS

with 123 more sequence matches

Only the first 10 are displayed here.

64.7%

NZ_CP085954.1:

Lanthipeptides: LK411_RS13585_lanthipeptide

ITSKFLCTPGCPTGGIACFTTQC--TNGCSIS

ITSK LCTPGCPTG + CFT C TNGC+I+

ITSKSLCTPGCPTGALGCFTQNCDPTNGCTIT

61.8%

NZ_CP113914.1:

Lanthipeptides: allorf_1411802_1411978_lanthipeptide

ITSKFLCTPGCPTGGIACFTTQCTNGCSI

+TS LCTPGCP+GG ACF+TQCTNGC

VTSHSLCTPGCPSGGFACFSTQCTNGCHF

58.8%

NZ_AULX01000006.1and 1 other:

NZ_LT732548.1 Lanthipeptides: CZ379_RS19505_lanthipeptide

Lanthipeptides: H583_RS0100280_lanthipeptide

ITSKFLCTPGCPTGGIACFTTQCTNGCSISVAC

ITSK LCTPGC TG + CF T GC IS +C

ITSKSLCTPGCITGVLMCF----TKGCEISHSC

52.9%

NZ_CP034587.1:

Lanthipeptides: EKH77_RS16870_lanthipeptide

ITSKFLCTPGCPTGGIACFTTQCTNGCSIS

ITS LCTPGCP+ C T+ CT GC IS

ITSISLCTPGCPSAA-GCTTSVCTQGCHIS

52.9%

NZ_CP086322.1:

Lanthipeptides: K9S39_RS23570_lanthipeptide

ITSKFLCTPGCPTGGIACFTTQCTNGCSIS

ITS LCTPGC T C T +CT GC IS

ITSVSLCTPGCVTAA-GCTTAKCTQGCHIS

52.9%

NZ_GG667191.1and 1 other:

NZ_GG667191.1 Lanthipeptides: HMPREF0298_RS11685_lanthipeptide

Lanthipeptides: HMPREF0298_RS11685_lanthipeptide

ITSKFLCTPGCPTGGIACFTTQCTNGCSISVA

ITSK LCTPGC TG + C T T GCS ++

ITSKSLCTPGCITGWMMCNTV--TKGCSFTIG

52.9%

NZ_JADNFO010000025.1:

Lanthipeptides: I2C89_RS09745_lanthipeptide

ITSKFLCTPGCPTGGI-ACFTTQCTNGCSISVA

ITS LCTPGC TG + AC T CSI V+

ITSYSLCTPGCKTGVLMACHLKTATCNCSIIVS

50.0%

NC_013656.1and 20 others:

NC_020450.1 Lanthipeptides: LILO_RS03015_lanthipeptideNZ_CP009472.1 Lanthipeptides: LG36_RS03420_lanthipeptideNZ_CP010050.1 Lanthipeptides: QI18_RS03300_lanthipeptideNZ_CP015903.1 Lanthipeptides: LLUC08_RS03255_lanthipeptideNZ_CP024954.1 Lanthipeptides: CV702_RS02990_lanthipeptideNZ_CP066300.1 Lanthipeptides: LacL0098_RS03205_lanthipeptideNZ_CP098790.1 Lanthipeptides: M5C73_RS03405_lanthipeptideNZ_JAALEQ010000006.1 Lanthipeptides: HFD15_RS10120_lanthipeptideNZ_JAJONJ010000005.1 Lanthipeptides: LSG16_RS07195_lanthipeptideNZ_JAQDWM010000007.1 Lanthipeptides: PGU01_RS08180_lanthipeptideNZ_JAQDXV010000018.1 Lanthipeptides: PGT80_RS11850_lanthipeptideNZ_JAQDYA010000007.1 Lanthipeptides: PGT72_RS09560_lanthipeptideNZ_JAQDYA010000007.1 Lanthipeptides: PGT72_RS09560_lanthipeptideNZ_JAQEIZ010000019.1 Lanthipeptides: PGX18_RS12025_lanthipeptideNZ_JAQEOU010000022.1 Lanthipeptides: PGV42_RS12330_lanthipeptideNZ_JAQEOV010000015.1 Lanthipeptides: PGW09_RS10765_lanthipeptideNZ_MTJP01000006.1 Lanthipeptides: BW151_RS03795_lanthipeptideNZ_MTJR01000005.1 Lanthipeptides: BW153_RS02980_lanthipeptideNZ_MTJS01000002.1 Lanthipeptides: BW154_RS09850_lanthipeptideNZ_RAGL01000022.1 Lanthipeptides: D6108_RS11395_lanthipeptide

Lanthipeptides: LLKF_RS13210_lanthipeptide

ITSKFLCTPGCPTGGI-ACFTTQCTNGCSISVA

ITS LCTPGC TG + C T CSI V+

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVS

50.0%

NZ_CP015405.2and 7 others:

NZ_CP015405.2 Lanthipeptides: A4V09_RS08295_lanthipeptideNZ_CP015405.2 Lanthipeptides: A4V09_RS08290_lanthipeptideNZ_CP015405.2 Lanthipeptides: A4V09_RS08285_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06240_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06235_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06230_lanthipeptideNZ_CP053228.1 Lanthipeptides: HL650_RS06225_lanthipeptide

Lanthipeptides: A4V09_RS08300_lanthipeptide

ITSKFLCTPGCPTGGIA-CFTTQCTNGCSIS

ITSK LCTPGC TG + C T GC I+

ITSKSLCTPGCVTGILMTCPVQTATCGCQIT

50.0%

NZ_CP065984.1and 2 others:

NZ_CP095737.1 Lanthipeptides: MXM90_RS03205_lanthipeptideNZ_JAQDXJ010000021.1 Lanthipeptides: PGU19_RS11740_lanthipeptide

Lanthipeptides: I6I21_RS02785_lanthipeptide

ITSKFLCTPGCPTGGI-ACFTTQCTNGCSISVA

ITS LCTPGC TG + C T CSI V+

ITSISLCTPGCKTGALMGCNMKTATCHCSIHVS

MIBiG 4.0 matches for HMPREF1978_RS10280

50.0%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

ITSKFLCTPGCPTGGI-ACFTTQCTNGCSISVA

ITS LCTPGC TG + C T CSI V+

ITSISLCTPGCKTGALMGCNMKTATCHCSIHVS

23.5%

BGC0000582.4 (ADR72962.1):

Linaridin: cypemycin

TPGCPTGGIACFTTQCTNGCSI

TP PT +A F Q + C +

TPATPT--VAQFVIQGSTICLV

17.6%

BGC0002110.3 (QKV49894.1):

Lanthipeptide: birimositide

CFTTQCTNGC

C TT+CT+ C

CPTTKCTSKC

with 2 more sequence matches

16.2%

BGC0001229.4 (SBI_06989):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

CFTTQCTNGC

C TT+CT+ C

CPTTKCTSRC

8.8%

BGC0000517.4 (BAB04173.1):

Lanthipeptide: haloduracin β or haloduracin α

CSISVAC

C+++V C

CTLTVEC

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.80 out of 29.38

Confidence: medium

Lanthipeptides

TFBS Finder

Core peptide predictions

HMPREF1978_RS10280 - Class I

Cleavage pHMM score: 4.40

RODEO score: 31

Monoisotopic mass: 3249.5 Da

Molecular weight: 3251.8 Da

Number of bridges: 6

Alternative weights: (assuming N unmodified Ser/Thr residues); 3269.8 Da N = 1; 3287.9 Da N = 2; 3305.9 Da N = 3

TFBS definitions

Regulator	Description
FuR variant 1	Ferric Uptake Regulator

NZ_KE951506.1 - Region 1 - RRE-containing

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Location: 48,653 - 68,940 nt. (total: 20,288 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase glycosyltransferase

HMPREF1978_RS09080: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

HMPREF1978_RS10170: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001483.4	0.44	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0000623.3	0.43	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0002306.2	0.43	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0001645.3	0.43	ribosomal	lagmysin	Streptomyces sp. NRRL S-118
BGC0000765.3	0.43	saccharide	exopolysaccharide	Lactobacillus johnsonii
BGC0001549.3	0.41	ribosomal	citrulassin B	Streptomyces aurantiacus JA 4570
BGC0000504.5	0.41	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0001634.3	0.41	ribosomal	keywimysin	Streptomyces sp. NRRL F-5702
BGC0001864.3	0.41	ribosomal	listeriocytocin	Listeria monocytogenes SLCC2540
BGC0000575.5	0.41	ribosomal	lariatin	Rhodococcus jostii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001483.4	0.44	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0000623.3	0.43	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0002306.2	0.43	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0001645.3	0.43	ribosomal	lagmysin	Streptomyces sp. NRRL S-118
BGC0000765.3	0.43	saccharide	exopolysaccharide	Lactobacillus johnsonii
BGC0001549.3	0.41	ribosomal	citrulassin B	Streptomyces aurantiacus JA 4570
BGC0000504.5	0.41	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0001634.3	0.41	ribosomal	keywimysin	Streptomyces sp. NRRL F-5702
BGC0001864.3	0.41	ribosomal	listeriocytocin	Listeria monocytogenes SLCC2540
BGC0000575.5	0.41	ribosomal	lariatin	Rhodococcus jostii

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.01 out of 20.97

Confidence: medium

Additional weak hits (4):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 22.23 out of 31.73

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.74 out of 30.25

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.06 out of 27.55

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 21.71 out of 33.73

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: PqqD_RRE; Location: 11 - 86; Score: 29.3

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A