LOCUS       NZ_KE951497             9030 bp    DNA     linear   CON 11-DEC-2024
DEFINITION  Actinomyces graevenitzii F0530 Scaffold5, whole genome shotgun
            sequence.
ACCESSION   NZ_KE951497
VERSION     NZ_KE951497.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Actinomyces graevenitzii F0530
  ORGANISM  Actinomyces graevenitzii F0530
            Bacteria; Bacillati; Actinomycetota; Actinomycetes; Actinomycetales;
            Actinomycetaceae; Actinomyces.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Weinstock,G., Sodergren,E., Wylie,T., Fulton,L., Fulton,R.,
            Fronick,C., O'Laughlin,M., Godfrey,J., Miner,T., Herter,B.,
            Appelbaum,E., Cordes,M., Lek,S., Wollam,A., Pepin,K.H.,
            Palsikar,V.B., Mitreva,M. and Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2013) The Genome Institute, Washington University
            School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: High-Quality Draft
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: Velvet v. 1.1.06
            Genome Coverage                   :: 191x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000466185.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 10:46:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,747
            Genes (coding)                    :: 1,701
            CDSs (with protein)               :: 1,701
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 46
            CDSs (without protein)            :: 46
            Pseudo Genes (ambiguous residues) :: 0 of 46
            Pseudo Genes (frameshifted)       :: 5 of 46
            Pseudo Genes (incomplete)         :: 43 of 46
            Pseudo Genes (internal stop)      :: 4 of 46
            Pseudo Genes (multiple problems)  :: 5 of 46
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:20:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9030
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KE951497.1.
            Bacteria provided by Jacques Izard, and Floyd E. Dewhirst, (The
            Forsyth Institute, Department of Microbiology, 245 First St,
            Cambridge, Massachusetts, 02142).  Funded by NIDCR DE016937 'A
            Foundation for the Oral Microbiome and Metagenome'.
            Source DNA provided by Alice Kirega, Anne C. Tanner, Floyd E.
            Dewhirst, and Jacques Izard (The Forsyth Institute, Department of
            Microbiology, 245 First St, Cambridge, Massachusetts, 02142).
            Funded by NIDCR DE016937, 'A Foundation for the Oral Microbiome and
            Metagenome'.
            The bacteria was isolated from a human subject. The phylogenetic
            relative position is available at www.homd.org.
            Coding sequences were predicted using GeneMark and Glimmer3.
            Intergenic regions not spanned by GeneMark and Glimmer3 were
            blasted against NCBI's non-redundant (NR) database and predictions
            generated based on protein alignments. tRNA genes were determined
            using tRNAscan-SE and non-coding RNA genes by RNAmmer and Rfam. The
            final gene set is processed through several programs such as Kegg,
            psortB and Interproscan to determine possible function. Gene
            product names are determined by BER. Gene names are generated at
            the contig level and may not necessarily reflect any known order or
            orientation between contigs.
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            This work was funded by the National Human Genome Research
            Institute (NHGRI)/National Institutes of Health (NIH) grant
            5U54HG00496804 for characterization of this genome.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9030
                     /db_xref="HMP:1978"
                     /db_xref="taxon:1321817"
                     /host="Homo sapiens"
                     /isolation_source="oral cavity- mouth"
                     /mol_type="genomic DNA"
                     /organism="Actinomyces graevenitzii F0530"
                     /strain="F0530"
                     /submitter_seqid="Scaffold5"
     region          1..9030
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..9030
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9030
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2442:6434](-)"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(2443..6434)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     gene            complement(755..1564)
                     /locus_tag="HMPREF1978_RS05675"
                     /old_locus_tag="HMPREF1978_00001"
     CDS             complement(755..1564)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013245.5"
                     /locus_tag="HMPREF1978_RS05675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF1978_00001"
                     /product="ABC transporter permease"
                     /protein_id="WP_021604100.1"
                     /transl_table=11
                     /translation="MRSFFVGLTLYLRREFLELITYRLALILGIVSAAVGIIQFAFLAI
                     FLQQGNSFPSLENYGGNVMAFLVSGALFTGFMSSALSSFSQLITSEQRTGTLEAVLTTP
                     FSIGRVIAYAAVSSFIQLAVSTFLMIMLFGFVFSIPFNINLWQLILVMVIGYFPLVGLG
                     LAGGGVLLVTKRGDPVTWLLTTFTTLFSGVLYPVEILPQWLQNISWYVPTTRVLSALRG
                     ALTGYSSMFTLDTIYLLISSVVSMVLGLLVFRWGLNRAIWDGSTTSY"
     gene            complement(1567..2439)
                     /locus_tag="HMPREF1978_RS05680"
                     /old_locus_tag="HMPREF1978_00002"
     CDS             complement(1567..2439)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.5"
                     /locus_tag="HMPREF1978_RS05680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF1978_00002"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_081688368.1"
                     /transl_table=11
                     /translation="MIVKAMNVTLNYGKSTSPVLSGLHLEVSSGSVLPILGGNGTGKTT
                     LLKALSGLLRPRTGSIMIGESDLYKSRKIALMHLSSSLYSERSFYYRLSALENLRYFLS
                     LRGIYGIKASEAINLALERFDLQMLADTPFMHLSLGQRKKLGLARAFAAPAGLYILDEP
                     TANLDTKACDLVYRAIAEASANGCSILFSTHNVADLATSTGDVIYLKGGAAMRVPLETR
                     DSVTARKRLRITGSLDPELIVSIPEVSTVISPSEVEVDIPVGKSLGSVLLALERIGIEV
                     NEVIDGGDE"
     gene            complement(2443..3645)
                     /locus_tag="HMPREF1978_RS09400"
                     /old_locus_tag="HMPREF1978_00003"
     CDS             complement(2443..3645)
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140: Lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017000.5"
                     /locus_tag="HMPREF1978_RS09400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF1978_00003"
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="WP_021604102.1"
                     /sec_met_domain="LANC_like (E-value: 5.9e-27, bitscore:
                     83.2, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSRAAVQNLATNVAKHLDNIVPVEYEDAQRGNHFNINGGTPGAI
                     LYLSVLDALFPDEGYDLRAHTYATTMISEYRLNPNIGIGLFGGTTLLAWCLRAISRDGT
                     RYQGAISDLESRIEFQITQYLTAIAKMDSPIPSEYYDVISGLSGVLLYSSRFRSPKLLS
                     TSQQIGNTFCDILSSWGVKGFGSLSAVSRQVSPGHEDETDYGYAHGLLGVLMSCLVSDS
                     TKNKDVLLTTELDKLIHLSTESNSSLLPYSSGNLQAGLSVGRYAWCYGNVPFYFLSQLL
                     DPVDPTRAQVLADLAVAPLSLAHGADRNDFGMIGLIDNSICHGRAGVLLGLQPSPVSAD
                     FDSIQTRLVEDIDYTLDEVNDGGFLEGDLGTIAVRLALECSSSIGRLPAFFPFVSPMLW
                     G"
     gene            complement(3642..6434)
                     /locus_tag="HMPREF1978_RS05690"
                     /old_locus_tag="HMPREF1978_00004"
     CDS             complement(3642..6434)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155: Lantibiotic dehydratase domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016615.5"
                     /locus_tag="HMPREF1978_RS05690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF1978_00004"
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="WP_081688369.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1.5e-27, bitscore:
                     84.8, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 2.3e-12, bitscore:
                     35.9, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHSYTILGKPYVRIAALPTYKFPSDGKAVSAFAKTVLSNPSLRSV
                     LASSTPTFYSAITKAISDGAFDDQTVSPKINRSALKYLSRLTSRATPFSTYATVGVLGD
                     FAWSADNLDLVISQKQILERVPHESCKDDQLYTFNQSAHISGDRVYIPSPSKNQETISV
                     RFSAAVESVKQITEGQIVSRELIASQLEEKFGTSDGLDDFIDGLISAELLLSLPTSYKI
                     TRWNEENKSSVNITSSVQLFSLGKFSGTLPIGLINDTAKAIELMGRLFSFSSQNRNIES
                     FANEFISRYGNFRFPASQIIDNYDGIGALPPLDNGSLELSEAGQARFELTNKLLTEILV
                     ANQGESSVDLSKYAGRIVENWPPIDFASSYDVIVKIESSTSQAGDSKAQFVLDDIGTIP
                     FSGRAMARFAELSSIMKQNHQKIVDAASCLEGAERLPLLYRYNSAKPDFLSGNLSVSGS
                     ALSLGYFPHATQDVSPVNLSDISLFELGGKMYCFDEKNQQIANIHTPGLVNQDLLSGVA
                     RTLVQISQMNTATPYWSWLTLDRLTSHLPEIRLGDMILCREKWRLTNRQFASVEDLKLL
                     LVELGIPRYIYTGASDNKILLDTEIVAHLQLLKHVIEFSDEDIWIERGVSPNALGITRS
                     LSDPNARYATELVLSIASDEWNGRKAPTVSHLPPVGLEPDLSKRSVLDSATAFTFVVFC
                     NESNQDRLLSTAFSILDDSGLKGYFVRYFEKGHASLRIRVKGRFDDVFIRSFCDLVLAQ
                     RLASDIEFNLRLPEFSRYGGTECFSFLEQFWCLESAQIVKNLNSQSLDSPEQIFASRYH
                     YLHCLVRYLGFDIEYVSAVADSYENEFVDDRHSVRKAARDVRDFFTDPTECHSLTPEMT
                     DLLTRFLSCELEGAVSLHDVKQSIVHLSANRLGLTRRDEAVFWRALFNRLRALQFGGTK
                     "
     gene            complement(6427..7050)
                     /locus_tag="HMPREF1978_RS05695"
                     /old_locus_tag="HMPREF1978_00005"
     CDS             complement(6427..7050)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005984426.1"
                     /locus_tag="HMPREF1978_RS05695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF1978_00005"
                     /product="hypothetical protein"
                     /protein_id="WP_021604104.1"
                     /transl_table=11
                     /translation="MKFLCSIERIAIIRWVTIAYIGALVLPLLFLRYGNTIAVAQSALL
                     IMLPIATAGLALSHAKVPASYVSFSIHDKVLLRFIAKSLLLAQVVNLCLSLLYVSIIGE
                     LVNESLSSHTALVLVFSSVVLTLACFALRLWTTSLVSWIVIIFFVVAGLPISTGDFANS
                     PVLFAVVPTTWILKGATFGSYIFFTGILLAAISLGFLLRSVKRA"
     misc_feature    complement(7095..7110)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 23.8"
                     /tool="antismash"
     gene            complement(7777..8379)
                     /locus_tag="HMPREF1978_RS05705"
                     /old_locus_tag="HMPREF1978_00007"
     CDS             complement(7777..8379)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005984428.1"
                     /locus_tag="HMPREF1978_RS05705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF1978_00007"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_231385553.1"
                     /transl_table=11
                     /translation="MSGINLQLNTGRIICLLGPNGAGKTTLMKTLLGRIKPVSGHIRYD
                     QTDISEMSAAIDHPSYFPYLSGKQNVQVVAAFWGLDVDPESALDSVGIVRAAHGRKAKD
                     YSTGMKERLELCLALLSRPKFLFLDEPQNGLDPDGMISLSATLRRYRDEGNCVVISTHL
                     LHEIQGVPDECVVVRNGKALHIPDLNGVNLADLYHRR"
     gene            complement(8519..8683)
                     /locus_tag="HMPREF1978_RS10280"
                     /old_locus_tag="HMPREF1978_00008"
     CDS             complement(8519..8683)
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR03731.1"
                     /locus_tag="HMPREF1978_RS10280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="HMPREF1978_00008"
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="WP_021604107.1"
                     /sec_met_domain="TIGR03731 (E-value: 1.7e-14, bitscore:
                     42.6, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATFDLDTRVEEAGETVRPQITSKFLCTPGCPTGGIACFTTQCTN
                     GCSISVACK"
     CDS_motif       complement(8624..8683)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MATFDLDTRVEEAGETVRPQ"
                     /locus_tag="HMPREF1978_RS10280_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(8519..8623)
                     /prepeptide="core"
                     /locus_tag="HMPREF1978_RS10280_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="4.40"
                     /molecular_weight="3251.8"
                     /monoisotopic_mass="3249.5"
                     /core_sequence="ITSKFLCTPGCPTGGIACFTTQCTNGCSISVACK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MATFDLDTRVEEAGETVRPQ"
                     /leader_location="[8623:8683](-)"
                     /alternative_weights="3269.8"
                     /alternative_weights="3287.9"
                     /alternative_weights="3305.9"
ORIGIN
        1 accagtaata gcttgcattg ctagctagtc gactccaact tctatcttgt gaccggatct
       61 taaaggcaaa ccagatagca tcggcagcga tggtggcatg atcgattagt gcgttgaact
      121 gacgcgactc aaagtccaga gttacaccac tatcagggaa ctgatcataa acagtaagga
      181 tggccctgca cccttatctg atcgtcttca attatcctgg cgtatcctcc tccatcgcag
      241 tagtgcagcc atcggggtgg acctcggcta cgaaacggcg cttggatgct agccatgaag
      301 gtatgcggcc aggcttgatc aagactcggc cagagaccag cgatagccac tcgtgttcat
      361 tgtcgacacc aggtaaaccg aatcccagaa attgatggga accagttgaa tctagttcga
      421 gcatggaacg agcctgccgt tcatctctgt catcaacact gaagttcgca aggttccact
      481 gctagctgca gcaagattca tttggttcat aacgttcgga atttttaaga gcatgtatgg
      541 gccagacacc gctacacgaa aaattgctta caccctatga caccgcccat gtttgtggag
      601 cctcttaggt catgcgcttc tactttcact ggtaaaccag cacctgcatc tcggacgctc
      661 tggttattaa gccaaattgc ggcctcgatc actgctctcg cttccgacct gcactaaagc
      721 aaattaacgc attagactta gtttcactgc tgccctagta actggttgta gagccatccc
      781 atatggctcg attaagaccc catctaaata caagcaagcc taaaaccatg ctgacgacgc
      841 tcgaaattaa aagataaatc gtatccagag taaacatact ggaataacca gttaatgccc
      901 cacgcagtgc gctcaacact cgagtagtcg gcacatacca actaatgttt tgaagccact
      961 ggggaagaat ctccaccggg tagagtaccc cagagaatag ggtcgtaaag gtcgtaagca
     1021 gccatgtaac aggatcccct ctcttagtaa ctagcaaaac ccccccgcca gctagcccaa
     1081 gcccgacaag cggaaaatat ccaattacca taactaaaat cagctgccac agatttatgt
     1141 taaaggggat tgaaaagaca aatccaaaca acatgatcat gagaaatgta ctgaccgcaa
     1201 gctggataaa agagctaaca gcagcataag ctataacacg tccaattgag aagggagtgg
     1261 tcagtactgc ctccagtgta cctgtccgct gctcagaggt aattagctga gagaagctag
     1321 aaagcgccga gctcatgaat ccggtaaaga gtgcaccgga aactaagaaa gccatcacat
     1381 taccgccgta gttctccaac gacggaaagc tgttaccttg ctgcagaaaa atggccaaga
     1441 atgcgaattg aatgatgccg acagcggcgg aaacaatacc taggatcaaa gcgagtctat
     1501 aggtgataag ctcaagaaac tcacgtctta agtacaatgt gaggccgaca aaaaaagatc
     1561 gcatttctat tcatcaccgc catcaatcac ttcgttgacc tcaatcccga ttcgctctag
     1621 tgcaaggagc acgcttccaa gagactttcc aacgggtatg tctacctcaa cttcagacgg
     1681 agagatcaca gtcgatactt caggaattga tacgatcagc tcaggatcaa gcgaaccggt
     1741 tattcttaaa cgcttacgag ctgtaacaga atcacgagtc tcaagtggta ctcgcatggc
     1801 agcccctcct ttgaggtaga taacatcgcc cgtggatgta gctaaatccg ccacattatg
     1861 cgtggagaat agaatgctgc agccattagc actagcttcg gcaatagcac ggtatacaag
     1921 atcgcatgcc ttggtatcta agttagcagt tggttcatcc aatatatata gacctgctgg
     1981 cgcagcaaat gcgcgtgcaa ggcctagttt ttttcgttgt ccaagtgaca aatgcataaa
     2041 tggagtgtct gcaagcattt gcaagtcaaa ccgctctaga gctaagttga tagcctccga
     2101 tgcctttatc ccatagatac cgcgaagtga caggaagtat cgtaggtttt cgagtgctga
     2161 aagacgatag tagaaagatc tctctgagta caacgaactt gaaagatgca ttaatgcaat
     2221 ttttcttgac ttatagagat cagattcgcc aatcatgata ctccctgtac gtggacgcaa
     2281 tagccccgac aacgccttaa gcaatgtggt ctttcctgta ccatttcctc cgaggatagg
     2341 aagaacactg ccggagctca cctcgagatg caagccagat aatacggggc ttgttgactt
     2401 gccataattg agtgtgacat tcatagcttt tacgatcatg tctcaacccc aaagcatagg
     2461 agatacaaac ggaaagaagg cgggtaatcg accaatggaa ctactgcatt caagcgctaa
     2521 tcgaaccgca atagttccta agtctccctc aagaaaccct ccatcattaa cttcgtccaa
     2581 agtgtaatcg atgtcttcca ccaggcgagt ttgaatcgaa tcaaagtcgg cactgactgg
     2641 ggatggctga agccccagga gcacccctgc acgaccgtgg caaatcgagt tatcaatcaa
     2701 gccgatcatc ccgaagtcgt ttcggtcagc accgtgagca aggcttaatg gtgcaacagc
     2761 taagtctgct aaaacttgtg ctcttgttgg atcaaccggg tcgagcaatt gagagagaaa
     2821 atagaacggc acattgccgt agcaccatgc gtagcggcca acactcagtc ctgcttgtaa
     2881 gttccctgaa gagtaaggga gcaaagagga gttcgattca gttgaaagat gtatgagttt
     2941 gtctaattca gtggtgagta ggacatcctt attctttgtg gaatcagaaa ctaagcaact
     3001 catcaggact cccaagagtc catgtgcgta accataatcg gtttcatctt catgaccagg
     3061 ggatacttgt cttgacacag cagagaggga gccaaagcct ttaacacccc aactactaag
     3121 aatgtcacaa aaagtattac caatctgctg acttgtagat agtagcttag gtgaccggaa
     3181 ccgagagctg tatagaagta ctcccgaaag accagagata acgtcataat actctgatgg
     3241 gatggggcta tccatcttag cgatggcagt caagtactgt gtaatttgaa actcgattcg
     3301 agactcaaga tcgctgatgg caccctgata tcgggtgcca tcacgtgaaa tcgccctcaa
     3361 acaccaagcc aaaagtgtcg tacccccaaa taacccgatt ccaatgttag gatttagcct
     3421 atattccgaa atcattgtcg tggcataggt atgggctcga aggtcgtaac cctcgtcggg
     3481 aaataatgca tcaagtacac ttaagtagag gatcgcaccc ggcgttcctc cattaatatt
     3541 aaagtggtta ccacgctgcg catcctcgta ttctacagga acaatgttgt caaggtgttt
     3601 tgccacattt gttgctagat tctgaacggc ggcccgagac ttcattttgt acctccgaac
     3661 tgtaatgctc ttaatcggtt aaaaagagcc cgccagaata ccgcctcatc cctccgagtc
     3721 aatccaaggc gatttgcaga taaatggacg atagactgct ttacatcatg tagtgagacg
     3781 gctccctcga gttcacaaga caaaaaccgg gttagaagat ccgtcatttc aggcgtcagt
     3841 gaatgacact ccgtgggatc ggtaaagaag tcacgaacgt ctcgcgcggc ctttcttacg
     3901 gaatgtcggt catccacaaa ctcgttttcg taactatccg ctacagcaga tacatattcg
     3961 atatcgaatc cgagataccg aacaaggcag tgtagatagt gatacctaga tgcaaatatc
     4021 tgttcgggcg aatctaaact ctgcgaattt agatttttga cgatctgagc gctctcaagg
     4081 caccaaaatt gttccaggaa gctaaaacat tcagtgcctc catatctcga aaactctggc
     4141 aacctaagat tgaactcaat atctgatgcc aatcgctgcg caagtaccaa gtcacagaaa
     4201 gaccggataa agacgtcatc gaagcgcccc ttcactcgta tgcgcaagga cgcatggccc
     4261 ttttcgaaat aacgaacaaa gtaccctttg agaccggaat catccaaaat actaaaggca
     4321 gttgacagaa gcctatcttg attggactca ttacagaata cgacaaacgt aaaagcagtg
     4381 gcgctatcaa ggacggaacg tttggatagg tcgggttcga ggccaacggg cggaaggtga
     4441 ctaaccgttg gggctttcct tccgttccat tcatctgacg cgatcgataa gactagctct
     4501 gtggcgtatc tggcatttgg gtcagacaaa gacctagtaa ttccaagagc gttggggcta
     4561 actcctctct ctatccaaat atcttcgtca gaaaactcaa tcacatgctt gagaagctgt
     4621 aggtgagcta caatttctgt gtcgagtaga atcttattat cagaggctcc cgtataaata
     4681 taccttggga ttccaagttc aacgagaagc agcttcaaat cttcgaccga cgcaaattgt
     4741 ctattagtta acctccattt ttcacggcag agaatcatat cccccagtcg aatctcaggc
     4801 aagtggctag tcagcctatc aagagtgagc caactccaat aaggagtcgc tgtgttcatt
     4861 tgagaaatct gaaccaaagt ccttgcaacc ccagataaca gatcctggtt cactaaccct
     4921 ggggtatgaa tatttgcaat ttgctggttt ttctcgtcga aacaatacat cttgccaccc
     4981 agttcaaaca acgatatatc ggagagattg acgggtgaaa catcttgggt agcatgagga
     5041 aaatatccta gagatagggc agacccagaa actgacaagt ttccgctcaa gaagtctggt
     5101 ttggcggagt tgtacctata cagaagagga agcctctcag caccctccaa acaagatgcc
     5161 gcatctacaa ttttctgatg attctgcttc attatcgaag aaagctcagc aaaacgtgcc
     5221 atagcacgcc cagaaaaagg aatagttcct atatcgtcaa ggacgaactg tgccttagaa
     5281 tcgccagctt gtgaggtgct actttcgatc tttacgatca cgtcatacga tgaagcgaag
     5341 tcaattggtg gccagttctc tactatacgt ccggcatact tactgagatc gacactactt
     5401 tcaccctgat tcgcaactaa aatttcagtc aggagtttat tcgttagttc aaatctggct
     5461 tgccccgctt cggacagctc tagacttccg ttatcgagcg gagggagagc gccaatacca
     5521 tcgtagttat caataatctg gctagcagga aaacgaaagt ttccatagcg agaaataaac
     5581 tcgttggcaa aagattcaat gttgcgattt tgggatgaaa atgaaaataa cctacccatc
     5641 agttcgatag cttttgctgt atcattaatc aaaccgatag gaagagtccc gctgaatttt
     5701 ccaagagaaa acagttgtac actggatgta atatttaccg aggatttatt ttcctcgttc
     5761 cagcgcgtga ttttatatga tgtcgggagc gataaaagta gctccgcaga gataaggccg
     5821 tcgatgaaat cgtcaaggcc atcgctggtg ccgaattttt cttccagttg cgaagcgatt
     5881 aactcgcgac ttactatttg tccttcggtt atttgtttta cagattccac tgccgcactg
     5941 aaccggacgg aaatggtctc ctgatttttt gagggcgatg ggatatatac acggtcgccc
     6001 gagatgtggg cagattgatt gaaagtgtat agctgatcat ctttgcaaga ttcgtgtggc
     6061 actcgttcca ggatctgctt ctgtgagatc actaggtcga ggttatcggc actccatgca
     6121 aaatccccaa gtactccaac ggttgcgtaa gttgagaagg gagttgctct agatgtgagt
     6181 cgagaaaggt actttaatgc gcttcggtta atctttggtg atactgtttg atcgtcaaac
     6241 gctccatcgg aaatagcctt cgtgatagcg gaataaaacg taggtgtgga acttgcaagg
     6301 actgatctca aactgggatt cgatagaaca gttttagcaa acgctgacac cgctttgccg
     6361 tcacttggaa acttatacgt tggaagtgca gctatcctca catacggttt tccaagtata
     6421 gtataactat gcacgtttca ccgacctcag taaaaacccc aaacttatcg cagccaacag
     6481 aatcccggta aaaaatatat aagagccaaa ggtggcacct tttaaaatcc aagtcgtggg
     6541 aacgacagca aacaagaccg gtgagtttgc aaaatcacct gtgctaatcg gaagacctgc
     6601 caccacaaaa aagatgataa caatccatga aactaatgaa gtagtccaaa gccgaagcgc
     6661 aaaacaggcc agagttagca caaccgaaga gaagacaagt actaaagcag tatgactcga
     6721 cagtgactca ttaacaagct cgccaatgat agagacataa agcagactca aacaaaggtt
     6781 aactacttgt gcgagaagca gcgatttagc aatgaaccgg agcagaactt tatcgtgaat
     6841 actaaacgag acatacgagg cagggacctt agcatgcgac aaagctagac ctgccgtggc
     6901 aattgggagc atgatgagta acgcggattg cgctacggca atggtatttc cataacggag
     6961 aaacagcagc ggaagaacta gtgcaccgat ataagcgatc gtgacccaac gaatgattgc
     7021 gattcgctcg atagaacaca aaaacttcat tttcttactg ctcctttact tcgaaaccca
     7081 gccacaagaa catagataat aattaggatt gctaccggga taagcgcaga gaaaccgaaa
     7141 tttgattgct cctggatatc tacgaacgag attgcggtac cattgagatt cagccctacg
     7201 gagtggaggg cgtttgatat gtatggggta gcattgtcag ctagattacc gggtagcact
     7261 atccagaagt acagaagaac acctgaaatt accgcggcag ccggaggtct tgagagactt
     7321 tctaatgtcg agtagatcag agcaattagt atactttgga aggtaagaaa gcaaagcagg
     7381 gtcgctagcc cagagtgcat cttcgaacca gacaaaacca tcgttagatc gaataacagc
     7441 acactaatca ggttcacaag aattattaca agcgttcgac agatagtgcg caggacgcta
     7501 ggcaaccaag caatgtatac ctgccgaggc aaacccccgt agtggacctt ggaaacccta
     7561 gcatcaagta taaagagtaa tgcgagggaa atcagtggct gcgttccagt aaagaagaag
     7621 ctgtatcccc ctgttattgg agttccgctt gcctcacttg aaatccatgc gccgaggaat
     7681 agcgacacca ttgagcccaa gatagccgcg agaaaatacc agatattcca atagtaagta
     7741 agcaaagcac ggatagctga agtgattggc atggccctac ctccggtgat ataggtccgc
     7801 gagattcacg ccgttgaggt cggggatatg taaagctttg ccgttacgaa ctacgacaca
     7861 ctcatccggg acgccttgaa tctcatggag gaggtgcgtt gatattacaa cgcagttccc
     7921 ttcgtcccga taacgacgaa gcgtcgcaga caaggagatc atgccatctg gatccaaacc
     7981 attctgtggc tcatcaagga acaaaaattt tggtctagat aataaagcaa gacaaagctc
     8041 cagccgttcc ttcatgccgg tcgagtagtc cttggctttc cgcccgtggg cagcgcggac
     8101 gatgcccaca ctatccaagg ccgactcagg atcaacatcc agaccccaga atgccgcgac
     8161 gacctgtaca ttttgtttac cactcagata cggaaagtag cttggatggt cgattgcagc
     8221 tgacatttca ctaatgtctg tctgatcgta tctaatgtgg ccacttactg gttttatccg
     8281 accaaggagc gttttcatta aggtggtttt acctgctcca tttggtccaa ggaggcagat
     8341 tatcctgcca gtatttagct gcaagttaat gccgctcact actggggaat tcttgtaccc
     8401 aaccgacaaa tcagttacag atatggctgg catggcctac ctttcaaaga aaacggccct
     8461 ggaggtacgg cctaggatgc cttacctcca gggccgactg ttgcgacaaa gcaactaact
     8521 acttgcaggc aacagagatg ctgcagccgt tagtgcactg ggtagtgaag cacgcgatgc
     8581 cgccggtcgg gcacccaggg gtgcacagga acttcgaggt gatttgagga cgaacagttt
     8641 ccccagcctc ttcaacacgg gtgtcaaggt cgaaggtggc catgacttct ccaatctctc
     8701 agaatctgaa catattcggg taacagaaag ttacccgcac cactatgcta caataaggct
     8761 aagccgaatg gaagactagc agggggcaac tttcagaatg ttcagatctg actatgattt
     8821 agcctaattg cgctccttcg agattgaggt aggtctacct gtttggacaa cctggcaata
     8881 gtaacataca aaatatagct aatgcaaacc atgatccagc aagggtgaag tactacctca
     8941 gcgcacgctt ggtccaaatc caacatgacc cggttttcca aactcggtct ccgagccttc
     9001 tttctggttg atctgtgaga acacgaggcc
//
