LOCUS       NZ_AUBE01000021        10399 bp    DNA     linear   CON 22-APR-2024
DEFINITION  Streptomyces flavidovirens DSM 40150 G412DRAFT_scaffold00019.19_C,
            whole genome shotgun sequence.
ACCESSION   NZ_AUBE01000021
VERSION     NZ_AUBE01000021.1
KEYWORDS    WGS; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces flavidovirens DSM 40150
  ORGANISM  Streptomyces flavidovirens DSM 40150
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 124519)
  AUTHORS   Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K.,
            Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A.,
            Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            environment                       :: Soil
            investigation_type                :: bacteria_archaea
            project_name :: Streptomyces flavidovirens DSM 40150
            sequencing_meth                   :: WGS
            ref_biomaterial                   :: DSM 40150, ATCC 19900
            finishing_strategy                :: Level 1: Standard Draft
            GOLD Stamp ID                     :: Gi11969
            Type Strain                       :: Yes
            Funding Program                   :: DOE-CSP 2012
            Isolation Site                    :: Soil
            Sporulation                       :: Sporulating
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Unknown program v. before 2013-03-07
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000429085.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 01:37:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,525
            CDSs (total)                      :: 6,452
            Genes (coding)                    :: 6,216
            CDSs (with protein)               :: 6,216
            Genes (RNA)                       :: 73
            rRNAs                             :: 6, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 106 of 236
            Pseudo Genes (incomplete)         :: 173 of 236
            Pseudo Genes (internal stop)      :: 25 of 236
            Pseudo Genes (multiple problems)  :: 59 of 236
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:04:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 86842
            Orig. end                         :: 97241
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AUBE01000021.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(79..90)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     misc_feature    115..130
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.74"
                     /tool="antismash"
     misc_feature    complement(462..477)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.81"
                     /tool="antismash"
     gene            complement(511..1605)
                     /locus_tag="G412_RS0123935"
     CDS             complement(511..1605)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123619.1"
                     /locus_tag="G412_RS0123935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amidohydrolase family protein"
                     /protein_id="WP_028814305.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 9.4e-19, bitscore:
                     60.6, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDRMVLRVKGRVLAGPDDVRDELWAVDGRITYERPVGVRDVPVV
                     HGWALPGLVDAHCHVGLDRHGPVDEATSEKQALTDREAGTLLIRDAGSPSDTRWIDDRD
                     DLPKIIRAGRHIARTRRYIRNYAHEIEPGDLVAYVAREARRGDGWVKLVGDWLDRETGD
                     LGACWPRGEVEAAIAQAHRLGARVTAHCFAEESLRDLVEAGIDCIEHATGLTEDTIPLF
                     AERGVAIVPTLVNIATFPHLADGGEAKFPRWSAHMRALHARRYDTVRAAYDAGVPVFVG
                     TDAGGSLAHGLVADEVAELVKAGIPAIDALSATAWGARRWLGRPLLEEGAPADLVVYDE
                     DPRADVRVLAAPRWIILNGRIVSG"
     gene            1682..2788
                     /locus_tag="G412_RS0123940"
     CDS             1682..2788
                     /NRPS_PKS="Domain: Aminotran_5 (74-295). E-value: 5.2e-28.
                     Score: 89.9. Matches aSDomain:
                     nrpspksdomains_G412_RS0123940_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104676.1"
                     /locus_tag="G412_RS0123940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_028814306.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.9e-22, bitscore:
                     72.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPLLDLAPLTAAHFAAIERRVAALLSTEQDVVIMQGEALLPLE
                     GCIRGGARPGSAALNVVTGPYGQTFGNWLRDCGAAVFDLAVPFHTAVTAEQIGQALDEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWERFAANPAAPRRSYLSLLDWKERWIDGGRRALLHAP
                     AQLEMLALEACLERIEAEGLGAVEARHLAAASATRAGAIALGGGLEPYVQAARDAAPVA
                     TTLRTPAGVDASQVVAKALAADPSLPLIAGGGALSKEMIRVNHYGVDATRGVVQSSLAA
                     LGAALSESGLRVDSEAARKAVAETWPSA"
     aSDomain        1904..2566
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G412_RS0123940_Aminotran_5.1"
                     /evalue="5.20E-28"
                     /label="G412_RS0123940_Aminotran_5.1"
                     /locus_tag="G412_RS0123940"
                     /protein_end="295"
                     /protein_start="74"
                     /score="89.9"
                     /tool="antismash"
                     /translation="RDCGAAVFDLAVPFHTAVTAEQIGQALDEHPEIDFVSLVHAEAAT
                     GNTNPVAEIGEVVRAHGALFMLDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVSA
                     VSVSERAWERFAANPAAPRRSYLSLLDWKERWIDGGRRALLHAPAQLEMLALEACLERI
                     EAEGLGAVEARHLAAASATRAGAIALGGGLEPYVQAARDAAPVATTLRTPAGVDASQV"
     misc_feature    2920..2935
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    3056..3065
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.92"
                     /tool="antismash"
     misc_feature    3067..3081
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.34"
                     /tool="antismash"
     gene            3090..3623
                     /gene="ectA"
                     /locus_tag="G412_RS0123945"
     CDS             3090..3623
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123622.1"
                     /locus_tag="G412_RS0123945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_078607305.1"
                     /transl_table=11
                     /translation="MTAAPADLARARSEFLQIEAPRVEDGAAIWRIARDSKALDLNSSY
                     SYLLWCRDFAATSVVARDSPGGEPIAFITGYIRPERPDTLVVWQVAVDHDHRGQGLAGT
                     LLEALSERVAARQGVKAIETTVTPDNTASNRLFTSYAERRGASVEREVLFDGGLFPEGT
                     HLPEVLYRIGPIAS"
     gene            3677..4945
                     /gene="ectB"
                     /locus_tag="G412_RS0123950"
     CDS             3677..4945
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 4.1e-71.
                     Score: 231.7. Matches aSDomain:
                     nrpspksdomains_G412_RS0123950_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="G412_RS0123950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_028814308.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.2e-76, bitscore:
                     250.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGSHLHDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFENVILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVEDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQDLCHRQDMLLIVDDIQMGCGRTGGFFSFEEAGITPDIVTLSKSISGYGMPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTATATLNTYWADGQMEKQTLARGEQVERALL
                     AIADENAGAGVSFRGRGLVWGLEFADKERATAVCRRAFELGMLLETSGPQSEVVKLLPA
                     LTISPDELDEGLRILARSVRETA"
     aSDomain        3761..4771
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G412_RS0123950_Aminotran_3.1"
                     /evalue="4.10E-71"
                     /label="G412_RS0123950_Aminotran_3.1"
                     /locus_tag="G412_RS0123950"
                     /protein_end="365"
                     /protein_start="28"
                     /score="231.7"
                     /tool="antismash"
                     /translation="FDRAQGSHLHDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMATTAKRAFLETFENVILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVEDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQDLCHRQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGITPDIVTLSKSISGYGMPMSLCLFKPELDVWEPGEHNGTFRGNNPAFV
                     TATATLNTYWADGQMEKQTLARGEQVERALLAIADENAGAGVSFRGRGLVWGLEFA"
     misc_feature    4991..5004
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.85"
                     /tool="antismash"
     gene            5001..5399
                     /locus_tag="G412_RS0123955"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="G412_RS0123955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_028814309.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.2e-55, bitscore:
                     178.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGETHWISPGTMYLLDGHERHTMRPKTDFR
                     CICVFNPPVTGREDHDENGVYPLLTEEV"
     gene            5403..6317
                     /gene="thpD"
                     /locus_tag="G412_RS0123960"
     CDS             5403..6317
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989365.1"
                     /locus_tag="G412_RS0123960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_051338567.1"
                     /sec_met_domain="PhyH (E-value: 1.1e-39, bitscore: 129.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDVLTARTETTRADLYRTRGAAEVITPRQDPVVWSAPGAPGPIE
                     PADLMGFERDGFLAIDQLITPEEVSVYRAELDRLVRDPGVRADERSIIEPKSQSVRSVF
                     EVHRISEVFARLVRDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHA
                     EDGLPNMRTVSVSIALTENYATNGGLMIMPGSHKEFVGCAGETPKDNYKRSLQMQDAGI
                     PSDEVLTRMADRHGIRLFTGKAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENTA
                     VEPFAAPVRRPEFIGARDFTPVR"
     gene            complement(6407..7477)
                     /locus_tag="G412_RS0123965"
     CDS             complement(6407..7477)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006349979.1"
                     /locus_tag="G412_RS0123965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alkene reductase"
                     /protein_id="WP_028814311.1"
                     /transl_table=11
                     /translation="MTTAFDTFDLSGTPLASRVVMAPMTRSRAGEGGVPTDLMVEYYAQ
                     RASAGLIVTEGVQPSVVGQGYPFTPGLHSAEQTEGWREVTDAVHAAGGKIYAQIMHSGR
                     IGHPVLLPDGLVPVAPSAVPAAGQLYTAEGMKDFVTPRELTGAEVRETISEYVTAARNA
                     IDAGFDGVELHGANGYLIHQFLSTNTNLRTDEWGGSAENRVRFAAEVTKAVAEAIGAGR
                     TGLRISPANPYNDIAETDTDLVYPALVEAVAPLGIAYLHVVEAAPRLRELTLELRAKFP
                     GAFILNPATEGPTDHGSLALVDEGVADLVAFGALFLANPDLPARLRTEGPYNTPDPASF
                     FGGDHRGYTDYPALAA"
     misc_feature    7565..7579
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.07"
                     /tool="antismash"
     gene            7596..8117
                     /locus_tag="G412_RS35710"
     CDS             7596..8117
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643608.1"
                     /locus_tag="G412_RS35710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_037649693.1"
                     /transl_table=11
                     /translation="MKAEPACPEPPSAASGGPVSHAISRAARLHRIAAGRLLRDLGLYP
                     GQEFVMMHLWDAGPVRQSELIKAVGLDPSTVTKMLQRLEQAGHVRRCPDPADRRAALVE
                     ATGASCGLKTQVEQAWRQLEETTLAGLDEHEREELLRLLGRVEGNLCPQAREEHEEAAD
                     AADAACVAEV"
     gene            complement(8192..9250)
                     /locus_tag="G412_RS0123975"
     CDS             complement(8192..9250)
                     /NRPS_PKS="Domain: Aminotran_5 (46-346). E-value: 1.4e-26.
                     Score: 85.2. Matches aSDomain:
                     nrpspksdomains_G412_RS0123975_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018553303.1"
                     /locus_tag="G412_RS0123975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_028814312.1"
                     /sec_met_domain="Aminotran_5 (E-value: 8.5e-23, bitscore:
                     73.7, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPLTLTDAVAAEFAPETTYLNTSTVGLPPRRTVAAVKAAAEQSA
                     TGQDAGDAFAPVSAARASFARIAGVPEDRVAVGTAVSVHVGLIAASLPAGAEVLCAEGD
                     FASVINPFAVRGDLKMRYVPLEELADSVRPETALVACSAVQSADGRAADLSAVRAAAAA
                     HGARTLVDATQAAGWLPFDAGSFDYTVTGGYKYLMAPRGTSFLTVTEEAQESLTPIHAG
                     WLAGEDIWGSTYGPVRELARGARRFDEPPPFLSYVGAEQSLALLEEIGVENVRDHNLAL
                     AARYREGLASLGHTPVPADSVVVSTPGLAHRLDALTSADIVVSVRAGNLRASFHLYNTG
                     ADVDRVLDVLAG"
     aSDomain        complement(8213..9112)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G412_RS0123975_Aminotran_5.1"
                     /evalue="1.40E-26"
                     /label="G412_RS0123975_Aminotran_5.1"
                     /locus_tag="G412_RS0123975"
                     /protein_end="346"
                     /protein_start="46"
                     /score="85.2"
                     /tool="antismash"
                     /translation="GQDAGDAFAPVSAARASFARIAGVPEDRVAVGTAVSVHVGLIAAS
                     LPAGAEVLCAEGDFASVINPFAVRGDLKMRYVPLEELADSVRPETALVACSAVQSADGR
                     AADLSAVRAAAAAHGARTLVDATQAAGWLPFDAGSFDYTVTGGYKYLMAPRGTSFLTVT
                     EEAQESLTPIHAGWLAGEDIWGSTYGPVRELARGARRFDEPPPFLSYVGAEQSLALLEE
                     IGVENVRDHNLALAARYREGLASLGHTPVPADSVVVSTPGLAHRLDALTSADIVVSVRA
                     GNLRASFHLYNTGADVDRV"
     gene            complement(9320..10291)
                     /locus_tag="G412_RS0123980"
     CDS             complement(9320..10291)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1015: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007268393.1"
                     /locus_tag="G412_RS0123980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_028814313.1"
                     /transl_table=11
                     /translation="MKPVQRVAVIVAPPVVSMFNVAIPEMLFPKAEVDGVPGYEVTACT
                     PDPGPVTTTGGLDLYVPRGLDAVDDADIVIVAGTGSLEEPDPRILAAVRRAAADGKRIA
                     SICTGAFVLAEAGLLDGRAATTYWTYTRELGDRYPAVDLQDEVLYVQDGPFMTSSGYAA
                     GIDLCLHIIRTDYGAAVANAVARLALVAPVRPGGQTQFTDTPLPPERGASFACTRAWAM
                     RHLDEPLTLTDLARHAGVSVRTLSRRFHAETGISPLQWLLHQRVERARELLETTTLPMD
                     RVARSSGLGTADSLRQHLLRKVGLTPSAYRSAFSRLTTTAAS"
ORIGIN
        1 cgcaggacgc tgaccgggtt gccctgctcc acgccgtcca actggaccgt gagtgcggtc
       61 ttttcggcgc tccccggagc gttcacttcg ttgagcgtgg tcgtgagcgg cacattgacg
      121 gtcttgttga gcagcgagat gtcgagcccg gtgcgcagga cgacggcgct cgccctgccc
      181 tcgccgccac ccccggtggc gtgcgccgcc ggagcggcga cgagcagagc ggcggcgggg
      241 cccgcggtca gggcgaaggc agccatggcg gccgcgggcc ggcgtgcgga catgctgaag
      301 aaggtgctgt tcaaagcggt ggaaccccca caagtgacac ggcgccgccc ggcgcactcc
      361 acgggacacg tgggcgccgg gacggctgag acacaggaat acttacgcac ttggagtgaa
      421 cggtcagcca cctgaggtga cttcactcct tcgtggggtt tccgagcccg cattcgaatt
      481 tcgcggggcc ctcgcgcacg cccgttcccc tcagccggac acgatccggc cgttcaggat
      541 gatccaccgc ggtgccgcga gcacgcgcac gtccgcgcgc gggtcctcgt cgtacaccac
      601 caggtcggca ggcgcgccct cctccagcag cgggcgcccg agccaccgcc gcgcgcccca
      661 tgccgtggcc gagagggcgt cgatcgcggg aatgcccgcc ttcaccagct ccgcgacctc
      721 gtccgccacc agcccgtggg ccagcgaacc gcccgcatct gtaccgacga agaccgggac
      781 gcccgcgtcg tacgccgcgc ggaccgtgtc gtaacggcgc gcgtgcaggg cccgcatgtg
      841 cgccgaccag cgggggaact tcgcctcgcc gccgtccgcg aggtgcggga acgtcgcgat
      901 gttgaccagg gtggggacga tcgcgacacc tcgctcggcg aagagcggga tggtgtcctc
      961 ggtcaggccc gtggcgtgct cgatgcagtc gatgcccgct tcgacgaggt cgcgcagcga
     1021 ctcctccgcg aagcagtgcg cggtgacccg ggcgcccagc cggtgcgcct gcgcgatggc
     1081 cgcctccacc tcgccgcgcg gccagcacgc gcccaggtcc ccggtctccc ggtcgagcca
     1141 gtcgccgacg agcttgaccc agccgtcgcc gcgccgcgcc tcgcgggcga cgtacgcgac
     1201 caggtcgccc ggctcgatct cgtgggcgta gttacggatg tagcggcggg tacgggcgat
     1261 gtgccgcccc gccctgatga tcttgggcag gtcgtcccgg tcgtcgatcc accgggtgtc
     1321 ggacggcgac ccggcgtcgc ggatcagcag cgtgcccgcc tcccggtcgg tgagcgcctg
     1381 cttctcgctg gtggcctcgt cgacggggcc gtgccggtcg agtccgacat ggcagtgcgc
     1441 gtcgaccagt ccgggcagcg cccagccgtg gacgaccggc acatcgcgta cgcccacggg
     1501 ccgttcgtac gtgatcctgc cgtccaccgc ccacagctcg tcccgtacgt cgtcgggccc
     1561 ggcgagcacc cgccccttca cccgcagcac catgcgatcg ctcatgtaca gcactctacg
     1621 agggggcggt tacgctcggc cgggagagat cgaacatccg aagatcgaag agagctccgc
     1681 cgtgacacac ccgcttctgg acctggcccc gctgaccgcc gcgcacttcg cggccatcga
     1741 acgccgggtc gccgccctcc tctccaccga gcaggacgtg gtcatcatgc agggtgaggc
     1801 gctgcttccc ctcgaaggct gcatccgcgg cggcgcccgt ccgggatcgg ccgcgctgaa
     1861 cgtggtgacc gggccgtacg ggcagacctt cggcaactgg ctgcgcgact gcggcgcggc
     1921 cgtcttcgat ctcgccgtcc cgttccacac ggcggtgacc gccgagcaga tcgggcaggc
     1981 gctcgacgag cacccggaga tcgacttcgt ctcgctggtg cacgcggagg ccgcgaccgg
     2041 aaacaccaac ccggtggcgg agatcggcga ggtcgtacgg gcccacggcg ccctcttcat
     2101 gctcgacgcg gtcgcttcgg tgggcgccga gccgctgctg ccggacgcgt ggggcgtgga
     2161 cctgtgcgtg atcggggcgc agaaggcgat gggcgggccg gccggggtct ccgcggtgtc
     2221 ggtgagcgag cgggcctggg agcggttcgc cgcgaacccc gccgccccgc gccgctcgta
     2281 cctctccctc ctggactgga aggagcggtg gatcgacggc gggcgcaggg cgctgctgca
     2341 cgcgcccgcg cagctcgaga tgctggcgct ggaggcgtgt ctggagcgta tcgaggcgga
     2401 ggggctcggc gccgtggagg ccaggcacct ggccgccgcc tcggccaccc gcgcgggcgc
     2461 gatcgcgctg ggcgggggcc tggagccgta cgtccaggcg gcgcgggacg ccgcgccggt
     2521 cgccacgacg ctgcgcaccc ccgccggggt ggacgcgtcg caggtcgtcg cgaaggcgct
     2581 cgccgccgac ccgtcgctgc cgctgatcgc gggcggcggg gcgctgtcca aagagatgat
     2641 ccgggtcaat cactacggtg tcgatgccac acggggtgtg gtccagtcgt cgctggccgc
     2701 actgggcgcg gcactgagcg aatcgggtct gcgggtcgac tccgaggctg cccgaaaggc
     2761 tgtggcggaa acctggccca gcgcataagt tctttgaatt cagtgaatta acggataacg
     2821 ggaagctgct ttatcagtgc agcttcccgt tttcttctgc cccgtatctc cgaccgtaaa
     2881 cgccaaggtt tcaacaggaa ctcgacccgg tttcaaccat gtgaccgact ccacatcgaa
     2941 ggcgatttgc ccggttattt ccggccaaat cgcccggatt tgcagtctgc tcgcgtgaag
     3001 ggcggtctcg cgcgataaca cagaacgcgt cctcacccaa ccccttccga cgatgcactt
     3061 tttgaatttg ctcggtaaat tccatccgca tgaccgccgc accagcagac cttgcacgtg
     3121 cccgaagcga attcctgcag atcgaagctc cgcgagtgga ggacggagcc gcgatctggc
     3181 gcattgcccg cgactccaag gccctggacc tcaactcctc gtacagctat ctgctgtggt
     3241 gccgcgactt cgcggcgacg tccgtcgtgg ccagggacag tcccggcggt gagccgatcg
     3301 cgttcatcac gggctacata cgccccgagc gccccgacac cctcgtcgtc tggcaggtcg
     3361 ccgtcgacca cgaccaccgc ggccaggggc tggccggcac gctcctggag gcgctgagcg
     3421 agagggtcgc cgcccgtcag ggggtgaagg cgatcgagac gaccgtgacc ccggacaaca
     3481 ccgcctccaa ccggctgttc acctcgtacg ccgagcggcg cggagcgtcc gtcgagcgtg
     3541 aagtgctctt cgacggcggg ctgttccccg agggcaccca cctgcccgag gtgctgtacc
     3601 gcatcggccc gatcgcttcc tgagcagccc tccccccttc cccccatact tcccccctct
     3661 ctcccaggag accgccgtga ccatcacccc tcccgccctg agcgtcttcg agaccctgga
     3721 atccgaggta cgcagctact gccgcggctg gccggccgtg ttcgaccgcg cgcagggcag
     3781 ccacctccac gacgaggacg gccatacgta cctcgacttc ttcgccggcg ccgggtcact
     3841 caactacggc cacaacaacc cggtgctgaa acgggcgctc atcgactaca tcgagcgcga
     3901 cggcatcacc cacggcctgg acatggcgac gaccgccaag cgcgccttcc tggagacgtt
     3961 cgagaacgtg atcctgcggc cgcgcgatct gccgtacaag gtgatgttcc cgggcccgac
     4021 gggcaccaac gccgtcgagg ccgcgctgaa gctggcccgc aaggtcaagg gccgcgagtc
     4081 ggtcgtctcc ttcaccaacg ccttccacgg catgtcgctc ggctcgctcg ccgtcaccgg
     4141 caacgcgttc aagcgggccg gcgccggcat accgctggtg cacggcacgc cgatgccgtt
     4201 cgacaactac ttcgacggca cggtcgagga cttcctgtgg ttcgagcggc tgctcgagga
     4261 ccagggctcc ggcctcaaca agcccgccgc ggtgatcgtc gagacggtgc agggcgaggg
     4321 cggcatcaat gtggcccgcg ccgagtggct gcgcgcgctc caggacctgt gccaccgcca
     4381 ggacatgctg ctgatcgtcg acgacatcca gatgggctgc ggccgtaccg gcggcttctt
     4441 ctccttcgaa gaggccggca tcacgcccga catcgtcacc ctgtcgaagt ccatcagcgg
     4501 ctacggcatg ccgatgtcgc tctgcctctt caagcccgag ctcgacgtgt gggagccggg
     4561 cgagcacaac ggcaccttcc gcggcaacaa cccggccttc gtcaccgcca ccgccacgct
     4621 caacacctac tgggccgacg gccagatgga gaagcagacg ctggcccgcg gcgagcaggt
     4681 cgagcgggcg ctgctggcca tcgccgacga gaacgccggg gcgggcgtga gcttccgggg
     4741 ccgcggcctg gtgtggggcc tggagttcgc cgacaaggag cgcgccacgg cggtctgccg
     4801 ccgcgccttc gaactcggca tgctgctgga gacctcgggt ccgcagagcg aggtcgtgaa
     4861 gctgctgccg gcgctcacca tctcccccga cgaactggac gaggggctgc gcatcctggc
     4921 ccgctcggtc cgcgagaccg cctgaccgtt ttcatcgcgg caacgaagta acgaagtacc
     4981 acagaagggc gcaactcacc gtgatagtcc gatcgttcaa ggacattgaa ggcaccgacc
     5041 gccatgtgaa ggccgcctcc gggacctggg agagcaagcg catcgtgctc gccaaggaga
     5101 aggtcggctt ctcgctccac gagaccgtgc tgtacgcggg tacggagacg tcgatgtggt
     5161 acgcgaacca catcgaggcc gtcctgtgca cggagggcga ggccgagctc accaacgacg
     5221 agaccggcga gacccactgg atctcgccgg gcacgatgta cctgctggac ggccacgagc
     5281 gtcacaccat gcgcccgaag accgacttcc gctgcatatg cgtcttcaac cctcccgtca
     5341 ccggacggga ggaccacgac gagaacggtg tctacccgct gctgaccgag gaggtctgaa
     5401 ccatgaccga cgtactgacc gcacgcaccg agaccacacg cgccgacctc taccggacgc
     5461 gcggcgccgc cgaggtgatc accccccgcc aggacccggt cgtctggtcg gcgcccggcg
     5521 cgccgggtcc catcgagccc gccgacctca tgggtttcga gcgggacggt tttctcgcca
     5581 tcgaccagct catcaccccg gaagaggtgt cggtctaccg ggcggagctg gaccggctgg
     5641 tcagggatcc gggggtgcgg gcggacgaac gctcgatcat cgagccgaag tcccagtctg
     5701 tacggtcggt cttcgaggtg caccgcatca gtgaggtctt cgcgcggctc gtacgggatc
     5761 cgagggtcgt cggcagggcc cgccagatcc tcggctcgga cgtgtacgtc caccagtcgc
     5821 ggatcaacgt caagccgggc ttcggggctt cgggcttcta ctggcactcg gacttcgaga
     5881 cgtggcacgc cgaggacggt ctgccgaaca tgcggaccgt gtccgtctcg atcgcgctga
     5941 ccgagaacta cgccaccaac ggcggcctga tgatcatgcc ggggtcgcac aaggagtttg
     6001 tgggctgcgc gggcgagacg cccaaggaca actacaagcg ctcgctccag atgcaggacg
     6061 ccggcatccc gtcggacgag gtgctgacgc gcatggcgga ccggcacggc atcaggctct
     6121 tcacgggcaa ggccggttcg gcgacctggt tcgactgcaa cgcgatgcac ggttcgggcg
     6181 acaacatcac gccgtacgcg cgcagcaatg tcttcatcgt cttcaacagc gtggagaaca
     6241 cggcggtcga gccgttcgcg gcccccgtac ggcggccgga gttcattggc gcgagggatt
     6301 tcacgccggt gcggtgaggt gaggccgggg ccgtgggcgg tcacggcgag gcgggcggga
     6361 ccggttcggt ggaaccggtc ccgcccgcct ctcgcgttcc ccgcgctcag gcggcgagcg
     6421 ccgggtagtc ggtgtagccc ctgtggtcgc cgccgaagaa ggaggccggg tccggggtgt
     6481 tgtacgggcc ttcggtccgc agccgggccg gcaggtcggg gttggcgagg aagagcgctc
     6541 cgaaggcgac gaggtcggct acgccctcgt cgacgagagc aagcgaaccg tggtccgtgg
     6601 ggccctcggt cgccgggttg aggatgaagg cgccggggaa cttcgcgcgc agctccagag
     6661 tcagctcgcg cagccggggg gcggcctcga cgacgtgcag gtacgcgatg ccgagcgggg
     6721 ccacggcctc gacgagcgcc gggtagacga ggtcggtgtc ggtctcggcg atgtcgttgt
     6781 acgggttggc gggcgagatc cgcaggccgg tccggccggc gccgatggcc tcggcgaccg
     6841 ccttggtgac ctcggcggcg aagcggacac ggttctcggc ggagccgccc cactcgtcgg
     6901 tgcgcaggtt ggtgttggtg gagaggaact ggtggatcag gtagccgttg gctccgtgca
     6961 gctcgacccc gtcgaagccg gcgtcgatgg cgttgcgggc ggccgtcacg tactccgaga
     7021 tggtctcgcg gacctcggcg ccggtcagct cgcgcggggt gacgaagtcc ttcatgccct
     7081 cggccgtgta gagctgcccg gcggcgggaa cggcggacgg ggcgaccggg accagtccgt
     7141 cgggcaggag cacggggtgt ccgatgcggc cggagtgcat gatctgggcg tagatcttgc
     7201 caccggcggc gtgcacggcg tcggtgacct cgcgccagcc ctcggtctgc tccgcgctgt
     7261 gcaggccggg agtgaacggg tagccctggc cgacgacgga gggctggacg ccttcggtga
     7321 cgatcaggcc ggcggaggca cgctgggcgt agtactcgac catcagatcg gtgggtacgc
     7381 cgccctcgcc ggcgcggctg cgggtcatcg gagccatcac gacgcggctg gccagcgggg
     7441 tgcccgaaag gtcgaacgta tcgaatgcgg tggtcatggc agaagcctcc gaaagagata
     7501 tgtggtcggc caagtaatcc ggcgagagcc actgtagcgc attacttggc cgaccaaggt
     7561 aaagttgttt cggacagttc cgacaaggag ttcccatgaa agccgagccc gcctgtcccg
     7621 agcctccgag cgccgcgagc ggcggcccgg tgagccacgc catctcccgg gcggcccggc
     7681 tccaccgcat cgcggcgggc agactcctgc gcgacctcgg cctctatccc ggccaggaat
     7741 tcgtgatgat gcacctgtgg gacgcgggcc cggtgcgcca gtcggagctg atcaaggcgg
     7801 tggggctcga cccgtcgacg gtgacgaaga tgctccagcg cctggagcag gcgggccacg
     7861 tacgccgctg ccccgacccg gccgaccggc gggccgcgct cgtcgaggcg accggtgcca
     7921 gttgcggcct gaagacgcag gtggagcagg cgtggcggca actggaggaa accacgctgg
     7981 ccggtctcga cgagcacgag cgcgaggaac tgctgaggct gctgggccgc gtggagggca
     8041 acctctgccc gcaggcccgc gaggagcacg aggaggcggc ggacgcggcg gacgcggcat
     8101 gcgtcgccga ggtttaggca cccggccatt gcgcccgtca ccgccggtcg ggcttgtcct
     8161 gttggagccc gaccggcggc gacagcgtcg ctcagcccgc gagcacgtcc agcacccggt
     8221 ccacatcggc gccggtgttg tacaggtgga acgacgcccg caggttgccc gcgcggaccg
     8281 agacgacgat gtccgccgag gtcagcgcgt cgagccggtg cgcgaggccc ggcgtcgaga
     8341 cgacgacaga gtcggcgggc accggagtgt ggccgaggga tgcgagccct tcgcggtagc
     8401 gcgcggcgag cgcgaggttg tggtcccgca cgttctcgac gccgatctcc tccaggagcg
     8461 cgagggactg ctcggcgccg acgtacgaca ggaacggcgg cggctcgtcg aaccggcgcg
     8521 ccccgcgcgc gagttcgcgg acgggaccat acgtgctgcc ccagatgtcc tcacccgcga
     8581 gccaccccgc gtggatcggc gtgagggact cctgcgcctc ttccgtgacg gtgaggaacg
     8641 acgtgccgcg cggcgccatc aggtacttgt agcccccggt gacggtgtag tcgaagctcc
     8701 cggcgtcgaa cggcaaccag cccgccgcct gggtcgcgtc gacgagcgta cgggcgccgt
     8761 gggcggcggc agcggcccgt accgccgaca ggtcggccgc ccgcccgtcg gcggactgca
     8821 ccgccgagca ggccaccagc gcggtctcgg ggcgcacgga gtcggccagc tcctccagcg
     8881 gcacgtaccg catcttgagg tccccgcgga ccgcgaaggg gttgatcacc gaggcgaagt
     8941 ccccctcggc gcacaggact tcggctccgg caggcagcga ggccgcgatc aggccgacat
     9001 ggacggacac cgcggtgccc acggcgaccc ggtcctcggg gacgccggcg atccgtgcga
     9061 aggaggcgcg ggcggcggac acgggggcga aggcgtcgcc ggcgtcctgg cccgtggcgc
     9121 tctgctcggc cgcggccttg acggccgcga cggtgcgacg gggcggcaag ccgacggtgg
     9181 acgtgttgag gtacgtcgtc tccggcgcga actccgcggc cactgcgtca gtgagagtaa
     9241 ggggcttcat gcccctactc tgctatgccg ccaactcccc gtccattgtg ttcttttgcc
     9301 gaatgtctcc aaggcatgct tatgaagcgg cggtcgtggt gaggcggctg aaggccgacc
     9361 ggtaggcgct gggagtcagg ccgaccttgc gcagcaggtg ctggcgcagc gagtcggccg
     9421 taccgagtcc gctggaacgg gccacccggt ccatgggaag cgtggtggtt tcgagcagtt
     9481 cacgggcgcg ctcgacgcgc tggtgcagca gccactggag ggggctgatc ccggtctcgg
     9541 cgtggaagcg gcgcgagagg gtgcgtacgg agactcccgc gtggcgggcg aggtcggtga
     9601 gcgtgagcgg ctcgtcgagg tgccgcatcg cccacgcgcg cgtgcaggcg aaggaggcgc
     9661 cgcgttcggg cggcagcggg gtgtcggtga actgggtctg accaccgggg cgtacgggcg
     9721 cgacgagggc gaggcgggcc accgcattgg cgacggcggc gccgtagtcg gtgcggatga
     9781 tgtgcaggca caggtcgatg ccggcggcgt agccggacga ggtcatgaac gggccgtcct
     9841 ggacgtagag gacttcgtcc tgaaggtcga cggccgggta acggtcaccg agttcgcggg
     9901 tgtacgtcca gtacgtcgtc gcggccctgc cgtcgagcag ccccgcctcg gcgaggacga
     9961 acgcccccgt gcagatggag gcgatgcgct tgccgtcggc ggcggcgcgg cgcacggcgg
    10021 ccaggatccg cggatcgggc tcctccagcg atccggtgcc ggcgacgatg acgatgtcgg
    10081 cgtcgtcgac ggcgtcgagt ccgcgcggta cgtacaggtc gaggccgccg gtggtggtga
    10141 cggggccggg gtccggggtg caggcggtca cctcgtaacc gggaacgccg tcgacctccg
    10201 ccttcgggaa gagcatctcg gggatggcca cgttgaacat cgagacgacg ggcggggcga
    10261 cgatcacggc gacgcgttgc accggcttca tggccagatc ctccggaggt atggcattcg
    10321 ggccactact ctagcgccgc cccggccgac aggcttggcg catggatacg aacaaccgcg
    10381 cccggacacc gcgggtgga
//
