LOCUS       NZ_AUBE01000005        13087 bp    DNA     linear   CON 22-APR-2024
DEFINITION  Streptomyces flavidovirens DSM 40150 G412DRAFT_scaffold00005.5_C,
            whole genome shotgun sequence.
ACCESSION   NZ_AUBE01000005
VERSION     NZ_AUBE01000005.1
KEYWORDS    WGS; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces flavidovirens DSM 40150
  ORGANISM  Streptomyces flavidovirens DSM 40150
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 420290)
  AUTHORS   Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K.,
            Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A.,
            Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            environment                       :: Soil
            investigation_type                :: bacteria_archaea
            project_name :: Streptomyces flavidovirens DSM 40150
            sequencing_meth                   :: WGS
            ref_biomaterial                   :: DSM 40150, ATCC 19900
            finishing_strategy                :: Level 1: Standard Draft
            GOLD Stamp ID                     :: Gi11969
            Type Strain                       :: Yes
            Funding Program                   :: DOE-CSP 2012
            Isolation Site                    :: Soil
            Sporulation                       :: Sporulating
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Unknown program v. before 2013-03-07
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000429085.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 01:37:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,525
            CDSs (total)                      :: 6,452
            Genes (coding)                    :: 6,216
            CDSs (with protein)               :: 6,216
            Genes (RNA)                       :: 73
            rRNAs                             :: 6, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 106 of 236
            Pseudo Genes (incomplete)         :: 173 of 236
            Pseudo Genes (internal stop)      :: 25 of 236
            Pseudo Genes (multiple problems)  :: 59 of 236
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:04:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 222554
            Orig. end                         :: 235641
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AUBE01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
FEATURES             Location/Qualifiers
     region          1..13087
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="2"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..13087
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13087
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:8087](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..8087
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            complement(<879..>1113)
                     /locus_tag="G412_RS41575"
                     /pseudo=""
     CDS             complement(<879..1113)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377608.1"
                     /locus_tag="G412_RS41575"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="LysR family transcriptional regulator"
                     /pseudo=""
                     /transl_table=11
                     /translation="MHTTEFQRRWHEQQPEKRPAPHPPQHSHRRPRRRTVRPRRHPCPD
                     RPETLVPRADRPQGPLRRSGVRRSLGPPPEYPP"
     misc_feature    complement(931..933)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1242..1556
                     /locus_tag="G412_RS41580"
     CDS             1242..1556
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="G412_RS41580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_028812017.1"
                     /transl_table=11
                     /translation="MAVRTRPTPSTDYLGYLGIGLAPLAATCWACYILLNRTVGARLPG
                     LEGSAAVAALSGVLYLPVGAWALWHHPPTPAALAIAVIVGANTAAVSTAHRSPTHRSTT
                     "
     gene            2010..2609
                     /locus_tag="G412_RS0110225"
     CDS             2010..2609
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007389714.1"
                     /locus_tag="G412_RS0110225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF998 domain-containing protein"
                     /protein_id="WP_028812018.1"
                     /transl_table=11
                     /translation="MQFVPRWALLSSGCAPVLLVGGWTIAALLEGPAYDPVTQTISVLA
                     AYGAAGFWVMTGAFLALGACHLLTAWALRAAALAGRVALGSGGVTAWVVALLPAPSSGG
                     SLRHGSVAAVGFALLAVWPLLAADRGGAAPWGLRPVPSIVATASMCVGAAWFMIEMHHH
                     GAAGVAERLVTSIQSLWPFVVVASCLRHSDHYRPSQ"
     gene            complement(2650..2805)
                     /locus_tag="G412_RS39590"
     CDS             complement(2650..2805)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="G412_RS39590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157839074.1"
                     /transl_table=11
                     /translation="MTWTNVTCVSRPSARVAIDIPEVLVHPRERTTQGGTATRNGAGVV
                     LKPAKA"
     gene            2867..3340
                     /locus_tag="G412_RS0110235"
     CDS             2867..3340
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1298: putative carboxymuconolactone decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010309000.1"
                     /locus_tag="G412_RS0110235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_028812019.1"
                     /transl_table=11
                     /translation="MDARLNYFSDPTADKFLKHFMAAGKALKSSPLPTATQELVALRVS
                     QINGCAVCIDMHTKEAAAAGETSARLHLVAAWREATVFTEAERAALELAEEGTRVADAA
                     GGVSDEVWANAAKHYDQEQLTALVILVSFMNTANRLNIMTQQPAGNYELGQFH"
     gene            3493..4413
                     /locus_tag="G412_RS33860"
                     /pseudo=""
     CDS             3493..4413
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059031.1"
                     /locus_tag="G412_RS33860"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="RNA polymerase sigma-70 factor"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSKVEEFEELRPLLFSIAYRILGSVGEAEDAVQETWLRFDGSATR
                     PTSTKAFLSATVTRISIDAAFRAGAAGGVRRAVASRAVAERSVSGSGAVGGAGRLGVDG
                     GAVAAGAAQPAGAGGVCAAGGVRLRVRRDGRGGGAFGGGVPAAGGAGAAAYGRRAAAVR
                     SGPSKRQELATRSFDALKNGDVGGLRNLLAADVLLIGDGGGKAPQLVKAVMGADNVARL
                     LGSVFPWLIEVDVTFELQEINGQPGAISRDRNGKVVHILALEVLDGQIQTIRGVINPDK
                     LGHLGPVADAWAIDREVKQARRRVN"
     misc_feature    complement(4467..4479)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.22"
                     /tool="antismash"
     misc_feature    4990..5007
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.31"
                     /tool="antismash"
     gene            5001..6155
                     /locus_tag="G412_RS0110245"
     CDS             5001..6155
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608091.1"
                     /locus_tag="G412_RS0110245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_028812020.1"
                     /sec_met_domain="DAO (E-value: 5.6e-68, bitscore: 224.5,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLTRDSSDVIVIGAGVVGAACAYYAARSGLTVTVLDRGPVAGGTT
                     GAGEGNLLVSDKEPGPELELALLSSQLWRELAELLPPVTEYEPKGGLVVASGEAGMAAL
                     RDFAAGQENAGVTAHEIPADRLHDLEPHLTPALAGGFHYPQDAQVQPALAAAHLLKAAG
                     ERVRLRLGEEVTGLLTGASGEVRGVRTASGEVHGPYVVNAAGTWGGELARLAGVELPVL
                     PRRGFVLVTEPLPRVVRHKVYAADYVADVASGSAALQTSAVVEGTPAGPVLIGASRERV
                     GYDRSLSVEVLRRLAAGATALFPALAGVRVMRTYAGFRPYLPDHLPAIGPDPRVPGLLH
                     ACGHEGAGIGLAPATGLVIARYLTGGEPLLDTAPFRPDRFPAAV"
     gene            6236..6550
                     /locus_tag="G412_RS0110250"
     CDS             6236..6550
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543028.1"
                     /locus_tag="G412_RS0110250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_028812021.1"
                     /sec_met_domain="Fer2_4 (E-value: 5.4e-26, bitscore: 85.0,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARTPAELVGAQPEPPFEITLDGRPVAALTGQSVAAALWAAGILA
                     WRTTRVGGRPRGAFCGIGQCYDCLATVNGEPNRRACLVPARPGDAITTQEGHGRADLSV
                     "
     gene            6531..8087
                     /locus_tag="G412_RS33865"
     CDS             6531..8087
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608089.1"
                     /locus_tag="G412_RS33865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_078606896.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 3.2e-26, bitscore:
                     86.7, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 5.2e-12, bitscore:
                     40.5, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTSASEPYDLAVIGAGPAGLSGAVTASESGLSVALLDTSGQVGG
                     QFYRHPAPALGAVRPEALHHDWSAFTELCRRQRASDIAHLAGHHVWAVTREGGGVWAVH
                     AVTGADGDGERPVCVRARAVLLATGAYERQLPFPAWTLPGVVGAGGAQAMLKSGLALPG
                     RRVVVAGSGPLLLAVASSLAAAGARVPAVVEASGYLRYARHPRVLAANPHKLAEAATHG
                     AALLRHGVRVRTRSAVIQVHGTERVEAVTVARLDPSWRPVQGSGRRIECDALAVGHGLV
                     PQIELATTVGCATRRTADGTTALALDDLQETSVSGLWAAGETGGVGGAQLAQIEGELAG
                     TAITARLLGRPRLARSGRVDELCHHRDRMRVFADVMAAAHAAGPGWAHWLTDDTDVCRC
                     EEVTAGRVREAIDDLGARDARTVKLLTRAGMGWCQGRMCGAAVACLAAGGGAVPPPDER
                     RPLAVPVPLGVLGALGSPQGAAVAAEGAEAVVVAEGLRAAEATERGAVPDAADPAGKAD
                     G"
     gene            8163..9071
                     /locus_tag="G412_RS0110260"
     CDS             8163..9071
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091456.1"
                     /locus_tag="G412_RS0110260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_028812022.1"
                     /transl_table=11
                     /translation="MTATTWNNSRPWRGIMVATALPLRADLSVDYDAYAEHVRRLIENG
                     CDGVVPNGSLGEYQTLTDEERARVVHTAVEAAGDGARVMPGVAAYGSAEARRWAEQAAE
                     AGAGSVLLLPPNAYRADEAAVRAHYADVARAGLPVVAYNNPIDTKVDLTPALLARLHGD
                     GHIVAVKEFTGDVRRAYEIAELAPDLDLLVGADDVLLELAVAGAVGWIAGYPNAFPATC
                     ATLYHAAMAGDLRTAVPLYKSLHPLLRWDSKTEFVQSIKLSMDIVGRQGGPVRPPRVPL
                     TGPVEAGVREATEKAVADGHH"
     gene            9144..10148
                     /locus_tag="G412_RS0110265"
     CDS             9144..10148
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="G412_RS0110265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="proline racemase family protein"
                     /protein_id="WP_028812023.1"
                     /transl_table=11
                     /translation="MRTRHVFHAVDSHTEGMPTRVITGGVGVIPGTTMAERRLHFMEHL
                     DHVRTLLMYEPRGHAAMSGAILQPPTRPDADYGVLYIEVSGVLPMCGHGTIGVATVLVE
                     TGMVPVTEPVTTVRLDTPAGLVTVDVQVTDGAATSVTLTNVPAFCAGLDRKVDVPGYGT
                     VTYDLAFGGNFYAFVRLDELGLPFDRTRKDDLLAAGLAIMEAINASPTRPVHPEQPDIA
                     GVKHVYLAAPGSDAVRSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGELPLGRDFVNE
                     SFIGTEFTGRLIGETEVGGVPAVVPTVTGRAWITGTTQYFLDPADPFPGGFLL"
     gene            10802..10984
                     /locus_tag="G412_RS41370"
     CDS             10802..10984
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /locus_tag="G412_RS41370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_279626764.1"
                     /sec_met_domain="Aldedh (E-value: 1.2e-07, bitscore: 25.1,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARTVPVQAEMGGLNAAVVLPDADIEQAAAHIAAAISGYGRPPPS
                     TDKARRSTSRCGRSR"
     gene            11198..11455
                     /locus_tag="G412_RS40775"
     CDS             11198..11455
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="G412_RS40775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_028812025.1"
                     /transl_table=11
                     /translation="MAALLPADDLAHAVRLTNSVPYDNSPSSGVDFHLPFGGVKAPKRQ
                     RAKASSHGHREQGRSALDFRTASRTYTPCHPLEQRDIVLW"
     gene            11480..12202
                     /locus_tag="G412_RS33875"
     CDS             11480..12202
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608085.1"
                     /locus_tag="G412_RS33875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_037648294.1"
                     /transl_table=11
                     /translation="MGHLTQRDLNATRERLRDQVAHALRAALISGELRPGQVYSAPALA
                     EDFGISATPVREAMLDLAREGLVEPVRNKGFRVTEVSESDLDQYTAIRALIEVPTVGQV
                     TRSATREELEALRPVAEEIVRAARDHDLIGYLEADRRFHLSLLALCGNERLVETVGDLR
                     KRSRLYGLTALDESGELISSADEHLELLDLMLAGDAKGAEKCMTRHLGHVRSLWARGES
                     AEGSGGRRSRLAKRAATG"
     misc_feature    12292..12302
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    12753..12767
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.35"
                     /tool="antismash"
ORIGIN
        1 gttcggaccg tgctcggcat cggtgctccc ggccgcgtcc gcactgtcgc ccgaatccgc
       61 gtgcgcgcac cggtcgggcc ggtagtactc ggaggccatg ctgcagatga agcccggcgt
      121 gcagtaaccg cactgggagc cgccgcggac cgccatctcc tcctgcaccg ggtgcagggc
      181 gggcggcgtg ccgggctcgc cgacggtggc gaggccttcg gaggtgatga tctcctgacc
      241 gtcgagcgcc gcggccggaa ccagacaggc gttgaccgcc acccagtcgg tgggcttgtg
      301 cacgccggga cgcgccacca ggaccgagca ggcgccgcat tcaccctcgg cacagccctc
      361 cttggttccg gtgaggccac gctcgcgcag gaaatccagc gctgtggtgt ggggcgcagc
      421 cggtgagatc ggtgcttctt tcccgttgac cgtgatccgc gcctctgtca tgacaggcct
      481 tcgctctggt gcgagatcgg tcgattcatt gttcgccaat ttcaggcagc tttctgtgtt
      541 cagacgcgcc acgtgagagg agcgggcgca agacggcacg taacagcgac gtgccggaag
      601 gaggtgacgg gaaacccgga atgtgccaca gacgggcaca tcagaacggc gtgctcagca
      661 gccgtgcgcg atccgacccg gaacccaggc tgtgcactgg gcgaggcgac caagatcaga
      721 gctgatgtac atctgctggt cgcctccttc cggtagttat gggtcgacga ttaccgcaaa
      781 ctagtggctt gggccacaga ggtcaagagg tttgccgcgg tctctcgagg accgatcgga
      841 ttcgaggctg gggcgccacc actgggcggt cgagcgccca ggcggatact ccggcggcgg
      901 gcccagggat cgtcggacgc cagagcgacg taacgggcct tgtggccgat cagcgcgtgg
      961 gaccaaggtt tcaggtcgat cggggcacgg atgacggcga ggtcgcacag tccttcggcg
     1021 aggccgccgg tgggagtgtt gtggcggatg aggcgcaggt ctcttctcgg gctgctgctc
     1081 gtgccagcgg cgctggaact cggtggtgtg ccggcggggc gatcgagcgg atcgggctcc
     1141 tactggccgt cacgctggag ttcctcggcc cgctgaccgt agccctggcc ggctcccgcc
     1201 gccgtatcga cctggtcgcc gcggtggcgg gtgcgggagc ggtggccgtc cgcacgcgcc
     1261 ccacaccctc caccgactat ctgggctatc tgggcatcgg cctggctccg ctggccgcga
     1321 catgctgggc ctgctacatc ctgctcaacc ggaccgtcgg ggcccgcctg cccggcctgg
     1381 aaggctcggc tgccgtagcg gccctctccg gcgtcctcta cctgcccgtc ggcgcgtggg
     1441 cgctgtggca ccacccgccc acgcccgccg cgctggccat cgccgtgatc gtcggagcta
     1501 acaccgccgc ggtctccacc gcacaccgtt cccccacgca ccgcagcacg acatgaaggt
     1561 catcatcgcc cgcgacctcg accccgagtc cttccagcgg cgcgacctcg ttgtgatctc
     1621 gcccggcttg gggttctcga tggtgtgcgg ttcgccgagc ggggcgaacg cctcggtgcc
     1681 gtccgcgtgt catccgcgtg tcggagcggc tgcggtgcct ggccagggct cgtggccgcg
     1741 caggccggtg gcggcgaggc tggtggtaca gcgaccgcat cccgctggcc aacgtgaaga
     1801 gatggtggcg aaccgcatcc gctcggccgc cgcttacggt cggcgccttc cggcatgcgc
     1861 ggggcggtcc gccgaccgac ggcgctccgg cccgccatca cttgccgttc gaccgtccag
     1921 gtcgcgttga gttgggcacc agcccattcg tattccactc gcccgcgtgt gcacgaggga
     1981 ggaatgcttg ggcgttttat ggttgtttga tgcaatttgt gcctaggtgg gccttgcttt
     2041 cgtcggggtg tgcgcctgtc ctgttggttg gaggttggac gatcgcggcg ttgctcgagg
     2101 ggcccgccta cgaccctgtc actcagacga tcagcgtctt ggcggcctac ggggctgcgg
     2161 gattctgggt gatgaccgga gcgttcctcg ccctgggcgc ctgccacctg cttaccgcct
     2221 gggcgctgcg cgcggccgcg ctcgccggac gtgtggcgtt gggcagcggg ggagtgacgg
     2281 cgtgggtggt ggctctgctc ccggcgccga gcagtggggg ttccttgcgg catggttccg
     2341 ttgccgcggt cggcttcgct ctcctggcgg tgtggccgct tctggccgcc gatcgcgggg
     2401 gagccgcacc atggggactc cggcccgtgc cctcaatcgt ggcgactgct tctatgtgcg
     2461 tcggcgcggc ctggttcatg atcgaaatgc atcatcacgg tgccgcaggt gttgctgaac
     2521 gtctcgtgac atccattcag tccctgtggc cctttgtggt cgtcgcttcc tgcctccgcc
     2581 actccgacca ttacagacct tcgcagtgac caggccgatg gacacttggt tgctgctgcg
     2641 ttcggccgcc tacgccttcg ccggtttgag gacgaccccg gcgccgtttc gagtggccgt
     2701 gccgccctgg gtcgtacgtt cgcgcggatg caccagcacc tcggggatgt cgatggcgac
     2761 gcgcgccgac ggccgggaga cgcaggtcac attcgtccat gtcacagggc gtcgggctac
     2821 cccgtctcag ggggtgtagc cactgacgac cacagaggag cacaccatgg acgcgcgact
     2881 gaactacttc tccgacccga ccgccgacaa gttcctcaag cacttcatgg cggcgggcaa
     2941 ggcgctcaag agctcgccgc tgccgaccgc cacacaggaa ctggtggcgc ttcgcgtaag
     3001 tcagatcaac ggctgcgccg tctgcatcga catgcacacc aaggaggccg ccgctgccgg
     3061 cgagacctcg gcgcggctgc acctggtcgc ggcgtggcgg gaggccacgg tcttcaccga
     3121 ggccgagcgt gccgctctgg agctggcgga ggagggaacg cgggtcgcgg atgcggccgg
     3181 tggggtcagc gacgaggtct gggcgaatgc cgccaagcac tacgaccagg agcagctcac
     3241 cgcccttgtg atcctggttt ccttcatgaa cacagcgaac cggctgaaca tcatgacgca
     3301 gcagcccgcc ggcaactacg agctcgggca gttccactga gcagcaccag caccagcaca
     3361 gcatcagcac cagcatcagt aactggctcg ggcgttgctc cgcgcctgcc ggtcgcccgg
     3421 atgatcacca gaggacgcct gcgccggcga gtacggccgg gtgggagcgt ctcaagggga
     3481 aggggattcg gcgtgagcaa ggtcgaggag ttcgaggagc tacggccgct gttgttttcg
     3541 atcgcctatc ggatcctggg cagtgtgggt gaggccgagg atgcggtcca ggagacatgg
     3601 ctgcgctttg acggctcggc gacccggccc acgtcgacca aggcatttct gtcggccacg
     3661 gtgacacgga tctcgatcga tgctgcgttc cgcgcgggtg cggcgggagg agtacgtcgg
     3721 gccgtggctt cccgagccgt tgctgagcga tccgtatcag gatccggcgc ggtcggtgga
     3781 gctggccgac tcggtgtcga tggcggcgct gttgctgctg gagcggctca gcccgctgga
     3841 gcgggcggtg tttgtgctgc gggaggtgtt cgccttcggg ttcgacgaga tggccgcggc
     3901 ggtggggcgt tcggaggcgg cgtgccggca gctggtggtg cgggcgcggc ggcatatggg
     3961 cgccgggcgg ccgcggttcg cagcggaccg tcaaagcgac aggagctggc gacgcggtcc
     4021 ttcgacgcgc tgaagaacgg cgatgtgggc gggctgcgga atctgctggc cgccgacgta
     4081 ctgctgatcg gggacggcgg cggcaaggcc ccgcagctgg tcaaggccgt catgggcgcg
     4141 gacaacgtgg ctcggctgct gggctcggtc ttcccctggc tgatcgaagt agacgtgacg
     4201 ttcgagctgc aagagatcaa tggccagcct ggtgcgatct cccgcgaccg gaacggcaag
     4261 gtggtgcaca tcctggccct tgaggtgctc gacggacaga ttcagacgat ccgaggggtg
     4321 atcaatcccg acaagctcgg ccacctcggg ccggtcgccg acgcctgggc catcgaccgc
     4381 gaggtgaagc aggcccgtcg gcgggtgaac tgacctcggg agtggcgtgg gctgtcgact
     4441 cctccaacag cagctcatgt cagcggccgc aaaatgcgac ccccggtgcc tcggacttgg
     4501 acaactgccc cttgaggcgc catggtcgct ggcgaaggcc tcggccttgc tggtcttgaa
     4561 cgtgccggaa cgaagcaagc gttaccaccg gcgccgcgac gcggccaccg caggctccgg
     4621 ttcggcgagg cggtcagcaa ctcgtagagg cggctgctca tgcggtttga cgttgggccg
     4681 gggcttattg cgccttgcca cgtatagccg gggtcgttcc tggctggtcg tcggtccgga
     4741 ccacacgagg cccctccctc gtgtggtctg ctcgatggtt cacagtcgcg agcttgccgg
     4801 ggccccccgc gcccatgacc cggttgcggg tcgggtcgaa ttctgcggag ttccgccgac
     4861 tgccgccccg acgttgcccg ttgccgggtt cgtcccattg gctctgccct ggccgccgcc
     4921 gggtgcttgg cccccagagc ggtcggttga ccggcgaggg ggtcttggca agcagtagtg
     4981 caatgtgaaa tgttacaggc gtgctcacga gagactcgtc agacgtcatc gtcatcggag
     5041 caggtgtggt cggcgccgcc tgcgcctact acgccgcccg gtccggcctc accgtcaccg
     5101 tgctcgaccg cggccccgtg gcaggtggca ccacgggtgc cggtgagggc aacctcctgg
     5161 tctccgacaa ggagcccgga cccgaactcg aactcgccct tctctcctcg cagttgtggc
     5221 gcgagctcgc cgaactgctg ccacccgtga ccgagtacga gcccaaaggc ggcctcgttg
     5281 tcgcctccgg agaggccggc atggccgcgt tgcgcgactt cgcagccggg caggagaacg
     5341 cgggggtcac ggcccacgag atcccggcgg accggctgca cgacctggag ccccacctca
     5401 cccccgctct ggcgggcggc ttccactatc cgcaggacgc ccaggtccag cccgcactgg
     5461 cggcggccca tctgctgaag gcggcgggcg agcgggtgcg gctgcggctc ggcgaggagg
     5521 tcaccggtct gctcaccggg gccagtggcg aggtccgggg ggtgcgcacg gcgtcggggg
     5581 aggtgcatgg cccgtacgtg gtcaacgccg ccggcacctg gggcggtgaa ctcgcccggc
     5641 tcgcgggggt ggagctcccc gtgctgcccc gcagagggtt cgtgctcgtc accgaaccac
     5701 tgccgcgcgt ggtgcggcac aaggtgtacg cggcggatta cgtcgccgac gtggccagtg
     5761 ggtccgccgc gctgcagacc tcggcggtgg tcgagggcac gcccgcgggg ccggtcctga
     5821 tcggcgccag ccgagaacgg gtgggctacg accggtcctt gtcggtcgag gtgctgcggc
     5881 gcctcgcggc cggggccacg gctctgttcc cagcgctggc cggggtgcgg gtgatgcgga
     5941 cgtacgcggg cttccgcccg tacctgccgg accacttgcc cgcgatcggt cccgatccac
     6001 gcgtccccgg gctgctgcac gcctgcggcc acgagggcgc gggaatcggc ctcgcaccgg
     6061 ccaccgggct ggtcatcgcc cggtacctga ccggcggtga accgcttctc gacacagccc
     6121 cgttcaggcc ggaccgcttc ccagccgccg tctgatccac ccgcgtccga gacccgcatc
     6181 cgagatccgt aaccgagacc cgcgtccgag acccgcacac gagaggaggc ccgcagtggc
     6241 ccgtaccccc gccgaactgg tcggggcgca gcccgagccg ccgttcgaga tcactctgga
     6301 cggacgtccc gtagccgccc tgaccggaca gtccgtcgcc gccgcgctgt gggcggcggg
     6361 catcctcgcc tggcgcacca cccgcgtcgg cggccggccg cgaggcgcct tctgcggcat
     6421 cggccagtgc tacgactgcc tcgccaccgt caacggagag cccaaccgga gggcctgcct
     6481 ggtcccggcc cgccccggtg acgcgatcac cacccaggag ggacacggcc gtgccgacct
     6541 cagcgtctga accgtacgac ctcgcggtca tcggcgcggg accagccgga ctgtccggcg
     6601 ccgtcaccgc ctcggaatcc ggcctgtcgg tcgcccttct cgatacgtcc ggccaggtcg
     6661 gcggccagtt ctaccggcat cccgcacccg cactcggagc ggtccgcccc gaggccctgc
     6721 accatgactg gtcggccttc accgagctgt gccgacgcca gcgcgcgagc gacatcgctc
     6781 acctggcggg gcatcacgtc tgggcggtga cgcgggaagg cgggggtgtg tgggcggtgc
     6841 acgccgtgac cggggccgac ggcgacggtg agcgtcccgt gtgtgtgcgg gcccgcgccg
     6901 tcctcctcgc taccggggcg tacgaacgtc aacttccctt ccctgcttgg accttgccgg
     6961 gtgtggtggg tgccggaggc gcccaggcca tgctcaagtc ggggctcgcg ctgcccggca
     7021 ggcgggtcgt cgtcgcgggc agcggcccgc tgctgctcgc cgtcgcctcc tcgctcgccg
     7081 ccgccggggc acgcgtcccc gccgtggtgg aggcgtccgg ctatctgcgg tacgcccgcc
     7141 acccccgcgt cctggccgcc aatccgcaca aactcgccga agcggcgacc catggcgccg
     7201 cgctgctccg ccacggcgtg cgggtgcgta cgcgcagtgc ggtcatccag gtgcacggca
     7261 ccgaacgggt ggaggccgtt acggtcgccc ggctcgaccc gagctggcgg cccgtacagg
     7321 ggtcgggccg tcgcatcgag tgcgacgcgc tcgccgtggg gcacggcctc gtgccccaga
     7381 tcgagctcgc caccacagtc ggctgcgcca cccgccgtac cgccgacgga accaccgccc
     7441 tcgccctgga cgacctccag gagacctccg tgtccggcct gtgggcggcg ggggagaccg
     7501 gcggggtcgg cggcgcccaa ctagcccaaa tagaagggga attggcgggc accgccatca
     7561 ccgcccggct gctcggacgg ccccggctcg cccggagcgg gcgcgtggac gaactgtgcc
     7621 atcaccgcga ccgcatgcgg gtcttcgccg atgtcatggc cgccgcccac gcggccggac
     7681 ccggatgggc gcactggttg accgacgaca cggacgtgtg ccgctgcgag gaggtcaccg
     7741 ccggacgcgt ccgcgaggcg atcgacgacc tcggcgcccg tgacgcgcgg accgtcaagc
     7801 tcctcacccg ggcgggcatg gggtggtgtc aggggcggat gtgcggtgcg gccgtggcct
     7861 gcctcgcggc cggcggtggc gcggtgccgc cgccggacga gcgccgcccg ctcgccgttc
     7921 ccgtcccgct cggggtgctc ggcgccctcg gctcgcccca gggggcggcc gtcgcggctg
     7981 agggggctga ggcggtcgtg gtggctgagg ggctgagggc ggctgaggcg accgagaggg
     8041 gcgccgtacc cgacgccgcg gatcccgccg ggaaggccga cggctgaacc tcccccgtgc
     8101 ccctccctcg cggcaccgcg ccatccaagt aaatgtcacc cgccatagaa gggctcaccg
     8161 gcatgaccgc caccacctgg aacaactccc gaccctggcg cggcatcatg gtcgccaccg
     8221 cgctccccct gcgcgcggac ctctccgtcg actacgacgc ctacgccgag cacgtccgcc
     8281 ggctcatcga gaacggctgc gacggcgtgg tccccaacgg ctcgctcggc gagtaccaga
     8341 ccctgaccga cgaggagcgg gcccgcgtgg tccacaccgc ggtagaggcc gcgggcgacg
     8401 gagcacgggt gatgcccggc gtcgccgcgt acggcagtgc cgaggcccgg cgctgggcgg
     8461 agcaggcggc cgaggcgggc gcgggctccg tactgctcct gccgccgaac gcctaccgcg
     8521 ccgacgaggc ggccgtacgc gcacattacg ccgacgtggc ccgggccggc ctccccgtgg
     8581 tggcgtacaa caaccccatc gacaccaagg tcgacctgac ccccgccctg ctcgcccggc
     8641 tccacggcga cgggcacatc gtggcggtga aggagttcac cggcgatgtc cgccgcgcgt
     8701 acgagatcgc cgaactcgcc ccggaccttg acctgttggt cggtgcggac gacgtcctgc
     8761 tggaactcgc cgtggccggt gcggtcggct ggatcgcggg ttaccccaac gccttcccgg
     8821 ccacctgcgc cacgctctac cacgcagcga tggccggaga cctgcggaca gcggttcccc
     8881 tctacaagtc gctgcacccg ctgctgcgct gggactcgaa gaccgagttc gtgcagtcga
     8941 tcaagctgtc catggacatc gtcgggcgtc agggcggccc ggtccgccct ccgcgcgtcc
     9001 cgctcacggg cccggtcgag gccggggtac gggaggcgac cgagaaggcc gtcgccgacg
     9061 gtcaccactg atcactggtc actgatcacc gatcacctga tcacccccga tcaccgatca
     9121 cctgatcgcc gaaaggatcc tccgtgcgca cccgccacgt cttccacgca gtcgactcgc
     9181 acaccgaggg catgcccacg cgcgtcatca ccggtggcgt cggggtgatc cccggcacca
     9241 ccatggccga gcgccgtctc catttcatgg aacacctcga ccacgtacgg acgctgctga
     9301 tgtacgagcc gcgtggccac gccgccatga gcggcgcgat cctgcagccg cccacccgcc
     9361 ccgacgccga ctacggcgtt ctctacatcg aggtctccgg tgtgctgccg atgtgcggac
     9421 acggcaccat cggggtggcc acggtcctcg tcgagacggg catggtgccg gtcaccgaac
     9481 ccgtcaccac cgtccgcctc gacaccccgg ccggtctggt caccgtcgac gtacaggtga
     9541 cggacggtgc ggccacctct gtcacgctca ccaatgtgcc cgcgttctgc gccgggctcg
     9601 accgcaaggt ggacgtcccc ggctacggga cggtgaccta cgacctcgcg ttcggcggca
     9661 acttctacgc cttcgtgcga ctggacgaac tcgggctgcc cttcgaccgt acccgcaagg
     9721 acgacctgct ggccgcgggc ctggccatca tggaggcgat caacgcgtcg ccgacccggc
     9781 cggtccatcc ggagcaaccg gacatcgccg gcgtcaagca cgtctacctg gcggcgcccg
     9841 gctccgacgc cgtccgctcc cgccacgcga tggccatcca tccgggatgg ttcgaccgct
     9901 cgccgtgcgg caccggcacc tccgcccgca tggctcagct ccacgcccga ggcgagcttc
     9961 ccctcggccg tgacttcgtc aacgagtcgt tcatcggtac ggagttcacc ggccggctga
    10021 tcggggagac ggaggtcggc ggtgttcccg cggtcgtccc caccgtcacc ggccgggcct
    10081 ggatcaccgg caccacgcag tacttcctcg acccggccga tccgtttccc ggaggcttcc
    10141 tcctgtgacc gcagacgtca cctctctcat ctcccccgcc atccggccga cccaaccgac
    10201 gtacccgtcc ggatcaccac ccctggtgcc ttcgccgcag tggataccgt ggggcgggcc
    10261 cgcgcggccc agcccggctg gctgaccggc ggggaggtcg cccggccgtc ggccctcggc
    10321 gcggtcggcg ccgccgtcga gggccgacgg ccgacgaact ggccgcgctc gccgcacgcg
    10381 aggcgggcaa gccgcttacc gaagccaggg ggccgaagtc gcgcggaccg ccgcgatctg
    10441 gcggtagtac gcccaggccc cttacgagcc caccggggcc agcccacgac cccgcagccg
    10501 gtgcggggct gctccttacc cgtcgccgcc cgcacggggt ggcgggcctg atcaccccgt
    10561 ggaaagttcc ccttcgccat cccgagctgg aggccgcacg cgccctcgcg gtaggcaacg
    10621 cggtcgtgct caagcccgct cccgagcaac ggcctgcgct cagcgcctcg tcgagccccc
    10681 caacagagcc tgcccgccgc cggtgtgttc acttgctgcc cggcggcgcc accgaaggca
    10741 acgcgctcgt ctccgccccg acgccgcctc cttcaccagc tcgaccacct tcggccgggc
    10801 gttggcgcgc accgtccccg ttcaggccga gatgggcggc ctgaacgcgg cggtggtcct
    10861 cccggacgcc gacatcgagc aggcggcggc ccacatcgcc gccgcgatct ccgggtacgg
    10921 tcggccgccg ccttcgaccg acaaggcgag gcgctcgacg agccgctgcg ggcggtcccg
    10981 gtaggggacc cggccgacgc ggcgacggta tgcgggccgc tcatcagcga acacgccctc
    11041 ggcaggtcag cgtctcctgg cacggtcttg cgcgctcgtc ggcggcaccg tctccccagg
    11101 ccccggctgg gacgcggcac ctggtacgcg gcacggaccc tggtcgagac ggtgccgcca
    11161 ggccaaccgg ctgctgcgag aagaggtctt cggtcccatg gcggccctgc tgcccgccga
    11221 tgacctcgcc cacgcggtac ggctcaccaa ctcggttccg tacgacaact ccccgtcctc
    11281 cggcgtcgac ttccacctgc cgttcggcgg cgtcaaggcg cccaagcgcc aacgcgccaa
    11341 ggcgtcaagc cacggccacc gcgaacaggg ccggtcggct ctggacttcc gcaccgcgag
    11401 tcgcacctac accccctgtc acccgctgga gcaacgtgac attgtactct ggtgatctcg
    11461 gcggtacgaa gggaacacca tggggcactt gacacagcgc gacctcaacg ccacccgcga
    11521 gcggctgcgc gaccaggttg cccacgccct gcgagctgcc ctgatctccg gcgaactgcg
    11581 gccgggccag gtgtactccg cacccgcact cgccgaggac ttcgggatct cagcgacgcc
    11641 cgtacgcgag gcgatgctcg acctggctcg ggagggcctg gtcgagcccg tccgcaacaa
    11701 gggattccgg gtcaccgagg tctccgagag cgacctggac cagtacacgg cgatccgcgc
    11761 cctgatcgag gtaccgacgg tggggcaggt cacccgcagc gccacccgcg aggagctgga
    11821 ggcgctgcgg ccggtcgccg aggagatcgt tcgggccgcc cgcgaccacg acctgatcgg
    11881 ctacctggag gccgaccgtc gctttcacct ctcgctgctc gcactctgcg gcaacgagag
    11941 gctggtcgaa accgtcggtg acctgcgcaa gcggtcccgc ctgtacgggc tcacagccct
    12001 ggacgagagc ggcgagctga tttcctcggc cgacgagcac ctggagctgc tcgacctgat
    12061 gctggcgggc gacgcgaagg gtgccgagaa gtgcatgacc cggcacctgg gccacgtccg
    12121 ctccctgtgg gccaggggtg agagcgcgga gggctccgga ggccggcgat cccggctggc
    12181 gaagcgggcc gcgaccggct gacaggggag gtctccataa cgcgcgttat gcgcacgtgg
    12241 gggcttcccg acaatccccg tccgcggtac agcccgaatc gtgcccagac acgcaatgtc
    12301 acaccgcaca tcgcgcggct actcgaccgg actcgaccgg gaaagggatc catgaacccc
    12361 gcgtacgcga ccgtcgatca cgtcttcacc acccccctgg accactcggc cccgcacggc
    12421 ccgaccatcc agctcttcgc ccgcgaggtg gccgatccgg gccgggtagg ggatcaactg
    12481 ccgtggctgc tctacctgca gggcggtccg ggaggcaagt caccccgccc gtacgccggt
    12541 tcacccggct ggctacccca tgcgctcaag acccaccgtg tgctccttct cgaccagcgc
    12601 ggcaccggcc gctccacacc cgtcacagcg cgggcggcct cccgcttctc ctccccggcc
    12661 cgactcgcca accacctgag ccactttcgc gcggacgcga tcgtcgccga cgccgaactg
    12721 atccgccggc ggctgtgcgg cgacgaaccg tgggagacgc tcggccagag ctacggcggc
    12781 ttcatcacgc tcacctatct gtcgcaggca ccagaagggc tgcgcgcctg ctacgtaacc
    12841 ggaggccttc ccggcctgtc cgccacagcc gacgacgtgt acgcgcgcac ctacccccga
    12901 gtacgggacc gcgtcctcga cttctacgcc cgctacccgg acgacgccgg ccggctgcgc
    12961 gagatcgccc gcatcctgac cgccaccgac gttcgcctgc ccgacggcga ccggctcacc
    13021 acccgccgcc tgctcaccct cggtctcgcc ctcggcatgg gggacggctt cgaacggatc
    13081 cactagc
//
