LOCUS       NZ_KE384533            10873 bp    DNA     linear   CON 02-MAY-2024
DEFINITION  Nocardioides alkalitolerans DSM 16699 K329DRAFT_scaffold00001.1,
            whole genome shotgun sequence.
ACCESSION   NZ_KE384533
VERSION     NZ_KE384533.1
KEYWORDS    WGS; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Nocardioides alkalitolerans DSM 16699
  ORGANISM  Nocardioides alkalitolerans DSM 16699
            Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
            Nocardioidaceae; Nocardioides.
REFERENCE   1  (bases 1 to 1380937)
  AUTHORS   Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K.,
            Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A.,
            Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T.
  CONSRTM   DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            country                           :: South Korea
            environment                       :: Soil
            investigation_type                :: bacteria_archaea
            isol_growth_condt                 :: 15774667
            project_name :: Nocardioides alkalitolerans DSM 16699
            sequencing_meth                   :: WGS
            ref_biomaterial                   :: DSM 16699
            rel_to_oxygen                     :: Aerobe
            finishing_strategy                :: Level 1: Standard Draft
            GOLD Stamp ID                     :: Gi11710
            Type Strain                       :: Yes
            Funding Program                   :: DOE-CSP 2012
            Isolation Site                    :: Alkaline serpentinite soil
            Cell Shape                        :: Rod-shaped
            Sporulation                       :: Nonsporulating
            Temperature Range                 :: Psychrotolerant
            Temperature Optimum               :: 25-30 C
            pH                                :: 5.5-12.0
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Unknown program v. before 2013-04-19
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000426525.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 01:43:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,653
            CDSs (total)                      :: 4,598
            Genes (coding)                    :: 4,584
            CDSs (with protein)               :: 4,584
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1 (5S, 16S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:19:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 751759
            Orig. end                         :: 762632
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KE384533.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 1013810
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Nikos   Kyrpides (nckyrpides@lbl.gov)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI
            Annotation by JGI
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10873
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10873
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10873
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5873](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5873)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(60..78)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.42"
                     /tool="antismash"
     gene            complement(437..2806)
                     /locus_tag="K329_RS0120410"
     CDS             complement(437..2806)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035: MMPL domain-containing transport protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386955.1"
                     /locus_tag="K329_RS0120410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MMPL family transporter"
                     /protein_id="WP_028474489.1"
                     /transl_table=11
                     /translation="MATLLYRLGKTAYRRWPLFMAGWFIALVAVGAVAATMSKPMTDNF
                     SIPGIPSEQAADLQAELFPGAMDPFDQANATVVVGVPEDAPADATLADPEYAEAIDALV
                     AELQEAPQIGDNAAAIVSPVAAVDALREQLAESGLDQAAIDEQVSRANIVSDDGRVGTI
                     PFVFDVDTVADVEPASQDAVLDLLDDARGDLVAEVNGAGMQSFAPPGATSEAIGIGIAL
                     IILIITFGSFVAAGLPILTAILGVGLSITGITALTAFMDIGSSTPILSTMIGLAVGIDY
                     TLFILARYRGELRHTDDRQEATGIAVGTAGSAVVFAGLTVLIALSALAVVGIPFLTAMG
                     VAAGATVLISVLVALTLLPAVLGLLKSKAFGGQVRKYSPKHDTDGRGRPQVLNNGVRWA
                     RIVGKRPLAVVLLVTVALGALAIPLKDLHLAFPTDSTAAADTTQRKASDLLTEAFGPGQ
                     EATFLFVVDGRELAEGDRQAAYDEVVSWVEGQEGIEYAVLAPSGGGEGAEGGTEGGGDP
                     AAAPVADGATIIGVPTTGPQDSATEDLLTNVRDGQSEIEEATGTTLGITGLTPITVDVS
                     ERLADALPVYLAVVVGLAFILLMIVFRSIIVPLTATLGFLLSVLATLGLTVLIFQEGTF
                     GIMDPQPIVSFMPIFLIGVVFGLAMDYQVFLVTRIREAHVHGADPREAVIDGFRNSARV
                     VAAAAAIMISVFAAFIAQDDAIIKSMGFALAVAVFLDAFIVRMTFIPALMYLLGDKAWY
                     LPKWLDRILPDVDVEGEKLDRAHLRAGDDEPATAGV"
     gene            complement(2890..3489)
                     /locus_tag="K329_RS29950"
     CDS             complement(2890..3489)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="K329_RS29950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_169721622.1"
                     /transl_table=11
                     /translation="MSPSADSESTDGRAARRQETTRRIVVTAQKLVLDRGLEGFTMDEL
                     AQATGVSRRTLFNYFPSKLDACLGPSPDLPDELMEAFRAGGPTGNLASDLEVVTRHVLG
                     QEPPTPEELQLGRALLQIPRFHQAVHERFEDLARKLQEGMQDRESEPVDPASGRLLVRL
                     LLAVVESAVDASVAGDDTPLPDLFSRQLARARALFA"
     gene            3600..4232
                     /locus_tag="K329_RS0120420"
     CDS             3600..4232
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355805.1"
                     /locus_tag="K329_RS0120420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nitroreductase family protein"
                     /protein_id="WP_028474490.1"
                     /sec_met_domain="PF00881 (E-value: 1.1e-10, bitscore: 38.2,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTDAAATTLRAPRPGAELLAEPMRSRWSPAVFATDAAIDDATLE
                     SLLVAASWAPSTGNTQPWAFVATRRGTASYDVVVDSLSAGNSGWVPTAPVLIVAATLVG
                     EHDGRSGKGDYTFYDLGQSVAHLTLQAQAVGLHTHQFAGFDHEAAARGLGIPASFRVLA
                     GIAVGYVGEDAHVEAAAPEDADRHRKERVRRALAETAYDGTWGTPLA"
     gene            complement(4271..4543)
                     /locus_tag="K329_RS0120425"
     CDS             complement(4271..4543)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K329_RS0120425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_028474491.1"
                     /transl_table=11
                     /translation="MTVVVVLTVVGVLALTATVVSFNRTARRSSHGRRRAGWAAGGGGA
                     TGGAYGCGSSGDSGHHSGGGDSGGGGDSGGSSCGSSCGGGCGGSS"
     gene            complement(4540..5004)
                     /locus_tag="K329_RS29955"
     CDS             complement(4540..5004)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="K329_RS29955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_028474492.1"
                     /transl_table=11
                     /translation="MTTGTARERLAARQEEVLAALLRGDVPPGVDAAGAGLTTRVLHRK
                     RSDGALRVVPELDELPGWRTAFHAWAAAHPADGCAHDDVRRFVAALRDGSVDVPTADDW
                     LGLHDVHEGRRRVALVRLAGRRTLVVGLGSHLWRLTPRRRGGALLEGTGS"
     gene            complement(5001..5873)
                     /locus_tag="K329_RS0120435"
     CDS             complement(5001..5873)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360431.1"
                     /locus_tag="K329_RS0120435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_245596627.1"
                     /sec_met_domain="DUF692 (E-value: 1.7e-64, bitscore: 213.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGLRGTAVAWRRPIADLLASYAAAGELSFTEVVAENVHPDRLPP
                     ALLALRGAGATVVPHGVTLGLGGADLPDPARLAHLAALATALGAPLVSEHVAFVRAATT
                     PDPLHGDVLEAGHLLPPARTADSLAVLVENVRAAQDALPVPLAVENIAALFTWPEDTLD
                     EADFLTALVDATGCRLVLDVANLHASATARGSDPVADLLRFPLDAVAYVHVAGGEEHAD
                     GLYLDTHGHPMTPPVLALLRTLVTAYDDRRLPLPGILLERDDDVTEAAVRSEYDVLRAA
                     AAPLVREHA"
     gene            complement(5870..6388)
                     /locus_tag="K329_RS0120440"
     CDS             complement(5870..6388)
                     /NRPS_PKS="Domain: Polyketide_cyc2 (0-149). E-value:
                     1.8e-13. Score: 43.0. Matches aSDomain:
                     nrpspksdomains_K329_RS0120440_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863150.1"
                     /locus_tag="K329_RS0120440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SRPBCC family protein"
                     /protein_id="WP_028474494.1"
                     /transl_table=11
                     /translation="MPQVSVSRWVPVTPDQAFAVSQTTGATRLRWDPFIRHQELLGADR
                     PAKGVRTLTRARVGPRMISEYVSYRPPTSVGMTMVKGPWFFASFGGGWRFTPEAREVDG
                     VTVEGAVAAWKYTYAVRPAWLRRVAEPIGQWLLGREIRARIDAFAGACADPVVLAVVTA
                     GPSPSGDPA"
     aSDomain        complement(5942..6388)
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_K329_RS0120440_Polyketide_cyc2.1
                     "
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="1.80E-13"
                     /label="K329_RS0120440_Polyketide_cyc2.1"
                     /locus_tag="K329_RS0120440"
                     /protein_end="149"
                     /protein_start="0"
                     /score="43.0"
                     /tool="antismash"
                     /translation="MPQVSVSRWVPVTPDQAFAVSQTTGATRLRWDPFIRHQELLGADR
                     PAKGVRTLTRARVGPRMISEYVSYRPPTSVGMTMVKGPWFFASFGGGWRFTPEAREVDG
                     VTVEGAVAAWKYTYAVRPAWLRRVAEPIGQWLLGREIRARIDAFA"
     gene            complement(6467..7399)
                     /locus_tag="K329_RS0120445"
     CDS             complement(6467..7399)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084897.1"
                     /locus_tag="K329_RS0120445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aldo/keto reductase"
                     /protein_id="WP_028474495.1"
                     /transl_table=11
                     /translation="MTSPATTTPTTLDLGPLMLGGNVFGWTADRDQGFAVLDAWLAAGG
                     RSIDTADMYPQWVDGRAIGDSERIIGEWITARGVRDQVVVATKVGQGDGREGLAPENLR
                     TALDESLERLQTDHVDLYYAHVDDESVPQAEYVAAFGALVAEGKALHLGASNFSAERLR
                     GAVALAAEQGVTGFTVSQDRWNLLARDIETDLVPTLAELGIQETPYSALASGFLTGKYA
                     AGAQVDSPRAGTAASYAQKPGADRVLAAQLEIASAHGVSPTAVALAWLRAQPVVSAPIA
                     SARNPEQLTALVESMTLELTADEVAALAG"
     gene            complement(7471..7821)
                     /locus_tag="K329_RS0120450"
     CDS             complement(7471..7821)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936235.1"
                     /locus_tag="K329_RS0120450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_028474496.1"
                     /transl_table=11
                     /translation="MTTEQPSDAPGPTVVENTDEHRFEIHVGGDLAGFATYELKDGDAG
                     PGTVVAILHTEIGESFAGQGLAKELAVQVLDGLRADGRQLLPYCPFFTKFVQKNAEYAD
                     LVPAARHEQFGL"
     misc_feature    complement(7849..7862)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.3"
                     /tool="antismash"
     misc_feature    complement(7934..7949)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.68"
                     /tool="antismash"
     gene            7976..9385
                     /locus_tag="K329_RS0120455"
     CDS             7976..9385
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753609.1"
                     /locus_tag="K329_RS0120455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD-dependent malic enzyme"
                     /protein_id="WP_028474497.1"
                     /transl_table=11
                     /translation="MPPISPPTPATASSYSITMRLHTAPDHGIVGTVATTISTAGGIVT
                     AIDVADSRHDRLVLDVTCSAGNADHSEQLVDAVRALEGVTVHKVSDRTFLLHIGGKIEV
                     TSKVPLKTRDDLSLAYTPGVGRVSRALAENPEDVSRLTIKGNSVAVVTDGSAVLGLGNI
                     GPGAALPVMEGKAALFKRFANIDAWPICLATQDTDEIVKAVEMIAPGFGGINLEDIAAP
                     RCFEIEARLRESLDIPVFHDDQHGTAIVVLAALTNALRCVGKQMETVKVVVAGAGAAGT
                     AIVTLLLAAGVTDVVVWDREGCLSGDDTSLPPAKLELAGRTNPRKVTGTLQDGLRGADV
                     FIGVSGPGLLQAEWIKDMADDSIVFALANPDPEIDPAEANKYAAIVASGRSDYPNQINN
                     VLAFPGVFRGLLDARASEVTVEALLRAAEAIALVVTDEELNPSFIIPSVFDAAVPKAVA
                     AAIAGEEHAKS"
     gene            9388..9693
                     /locus_tag="K329_RS0120460"
     CDS             9388..9693
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /locus_tag="K329_RS0120460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="WP_211225918.1"
                     /transl_table=11
                     /translation="MEHPEHDIEALVVDCHPVDGGDLACFRVLILLRREIEHLAPGAVV
                     CLTTTDPVAPIDLPAWCRLTGHEYLGPVPGRPTPTYGVRVAAAPHATRPDRPWHRA"
ORIGIN
        1 gagcgcatct cgtcgtacga cgacctcgcc gccgccgtcg cgacccacca gccgggggac
       61 gaggtgagcg tcacctggac atccgccgac ggccaggagc gcaccgccga cgtcaccctc
      121 ggggagtcgc cggtcaactg accgtcgccg acccgccgcg ccccacgcgc ggtccagggc
      181 cacggacgag ccgccttccc cgggcggctc gcccgtggcc cttcggctgt ccctggggag
      241 cccggtggtc gaggaggttg cgcagcaacc gtctcgagac ctgaggggtt gggtggtttc
      301 gagacgctcg ctggcgctcg ctcctcaacc accggggacg cggggtcgct cgacccccgg
      361 gggcccggca gacgaaccgg cggccgcgac accgaggtgt cgcgaccgcc ggcgggcgga
      421 gcggaggggg cggaggtcag accccggcgg tcgccggctc gtcgtcgccc gcgcgcaggt
      481 gcgcccggtc gagcttctcg ccctcgacgt ccacgtcggg gaggatccgg tcgagccact
      541 tcggcaggta ccaggccttg tcgccgagca ggtacatcag cgcggggatg aaggtcatcc
      601 gcacgatgaa ggcgtcgagg aacactgcga cggcgagcgc gaagcccatc gacttgatga
      661 tcgcgtcgtc ctgggcgatg aacgccgcga acaccgagat catgatcgcc gcggcggcgg
      721 ccacgacgcg ggccgagttg cggaacccgt cgatgacggc ctcgcgcggg tcggcgccgt
      781 gcacgtgggc ctcgcggatg cgggtgacga ggaagacctg gtagtccatc gcgagcccga
      841 agaccacgcc gatcaggaag atcggcatga agctgacgat gggttgcggg tccatgatcc
      901 cgaacgtgcc ctcctggaag atcaggacgg tgaggccgag cgtcgcgagc accgagagca
      961 ggaagccgag cgtcgcggtc agcggcacga tgatcgagcg gaacacgatc atcagcagga
     1021 tgaacgcgag accgacgacg acggccaggt agaccggcag cgcgtcggcg agccgctccg
     1081 agacgtcgac cgtgatgggc gtgaggcccg tgatgccgag cgtggtgccc gtggcctcct
     1141 cgatctcgga ctggccgtcg cgcacgttgg tgagcaggtc ctcggtggcc gagtcctggg
     1201 ggccggtggt cgggacgccg atgatggtgg cgccgtccgc gacgggtgcg gccgcggggt
     1261 ccccgccgcc ctcggtgccg ccctcggcac cctcgccgcc gccgctgggc gccagcacgg
     1321 cgtactcgat gccctcctgg ccctcgaccc aggagacgac ctcgtcgtac gccgcctgcc
     1381 ggtcgccctc ggcgagctcg cgcccgtcga cgacgaagag gaacgtggcc tcctggccgg
     1441 ggccgaacgc ctcggtgagg aggtcggagg ccttgcgctg cgtggtgtcg gcagcggccg
     1501 tggagtcggt cgggaacgcg aggtggaggt ccttcagcgg gatggcgagc gcgccgagcg
     1561 cgaccgtcac cagcagcacg acggcgagcg ggcgcttgcc gacgatccgg gcccagcgca
     1621 cgccgttgtt gagcacctgc gggcggccgc ggccgtcggt gtcgtgcttc ggggagtact
     1681 tgcggacctg gccgccgaac gccttcgact tgagcaggcc gagcacggcc ggcagcagcg
     1741 tgagcgcgac gagcaccgag atgagcacgg tggcgccggc ggcgacaccc atggcggtga
     1801 ggaacgggat gcccacgacc gcgagggcac tgagcgcgat gagcacggtg aggccggcga
     1861 agaccacggc ggagccggcc gtgccgaccg cgatgccggt cgcctcctgc cggtcgtcgg
     1921 tgtgcctcag ctcgccgcgg tagcgggcca ggatgaagag cgtgtagtcg atgccgaccg
     1981 cgagcccgat catcgtcgag aggatcggcg tcgacgagcc gatgtccatg aaggcggtca
     2041 gcgccgtgat gccggtgatg gagagcccga cgccgaggat cgcggtgagg atgggcaggc
     2101 cggcggccac gaacgagccg aacgtgatga tgaggatgat cagcgcgatg ccgatgccga
     2161 tggcctcgga ggtcgcgccc ggcggagcga agctctgcat gccggcgccg ttgacctcgg
     2221 cgacgaggtc gccgcgggcg tcgtcgagga ggtcgagcac cgcgtcctgc gaggccggct
     2281 cgacgtcggc cacggtgtcg acgtcgaaga cgaacgggat cgtcccgacg cgaccgtcgt
     2341 ccgagacgat gttggcgcgc gagacctgct cgtcgatcgc ggcctggtcc aggccggact
     2401 cggcgagctg ctcccgcagg gcgtccacgg cggcgacggg cgacacgatc gcggcggcgt
     2461 tgtcaccgat ctgcggggcc tcctgcagct cggcgaccag tgcgtcgatc gcctcggcgt
     2521 actccgggtc cgccagggtg gcgtccgccg gagcgtcctc gggcacgccc acgacgaccg
     2581 tggcgttggc ctggtcgaag ggatccatgg cgccggggaa gagctcggcc tgcaggtcgg
     2641 ccgcctgctc ggacgggatg ccggggatcg agaagttgtc ggtcatcggc ttgctcatcg
     2701 tcgccgcgac ggcgccgacg gcgacgagcg cgatgaacca gccggccatg aacagcggcc
     2761 atcgtcggta ggcggtcttg ccgagtcggt agagcagggt ggccatcggg tctcctggtc
     2821 gaggtccggg gtggggtacc ggggtggtcg ggcggtgcgg tgcggggcgg tgcggtgcgg
     2881 tgcggacggt caggcgaaca gcgcgcgggc gcgggcgagc tggcgtgaga agaggtcggg
     2941 gagcggggtg tcgtcgccgg cgacgctggc gtcgacggcg gactcgacga cggcgagcag
     3001 caggcggacg aggaggcggc ccgaggccgg gtcgacgggc tcgctctcgc ggtcctgcat
     3061 gccctcctgg agcttgcggg cgaggtcctc gaaccgctcg tgcaccgcct ggtggaagcg
     3121 ggggatctgc aggagggccc ggcccagctg cagctcctcg ggcgtcggcg gctcctggcc
     3181 cagcacgtgg cgggtgacga cctcgaggtc ggacgccagg ttgccggtcg gtccgccggc
     3241 gcggaacgcc tccatcagct cgtcgggcag gtcgggcgac ggaccgaggc aggcgtcgag
     3301 cttcgagggg aagtagttga agagggtgcg gcgggagacg ccggtggcct gggccagctc
     3361 gtccatcgtg aagccctcga gcccacggtc cagcaccagc ttctgggcgg tcaccacgat
     3421 gcgtcgggtc gtctcctgac gacgggcggc gcgaccgtcg gtcgactcgg aatctgcact
     3481 cgggctcacg gagtgcaagt ttgcactaag gggctctgag tgcaaagtgg ctcggtctgt
     3541 gacgcccgcc tcacccggtg acgtgaggcc gagcgggccg cctcgccaca atggacggca
     3601 tgtcgaccga tgccgccgcc accacgctcc gcgccccccg tcccggagcc gagctcctcg
     3661 ccgagccgat gcgctcgcgc tggagccccg ccgtcttcgc gaccgacgcc gcgatcgacg
     3721 acgccaccct cgagagcctc ctcgtcgccg cgtcgtgggc gccgtcgacc ggcaacaccc
     3781 agccgtgggc gttcgtcgcc acgcggcgcg gcaccgcgtc gtacgacgtc gtggtcgact
     3841 cgctgagcgc gggcaactcc ggctgggtgc cgaccgcgcc cgtgctgatc gtggcggcga
     3901 cgctggtcgg ggagcacgac ggccggtcgg gcaagggcga ctacaccttc tacgacctcg
     3961 gccagtcggt ggcgcacctg acgctccagg cccaggccgt cggtctgcac acccaccagt
     4021 tcgccggctt cgaccacgag gccgccgcgc gtggtctcgg gatccccgcg tcgttccggg
     4081 tgctcgccgg catcgcggtc gggtacgtcg gggaggacgc gcacgtcgag gcggccgcgc
     4141 ccgaggacgc cgaccgccac cgcaaggagc gcgtgcggcg tgcgctggcc gagacggcgt
     4201 acgacggcac ctggggcacc ccgctcgcct gagcggtccc cgggacgacc cgtccgagct
     4261 gcccctcggg tcagctgctc ccgccgcagc cgccaccgca ggacgatccg cacgagctgc
     4321 cgcccgagtc gcccccaccg ccggagtcgc ccccgcccga gtggtgcccg gagtcgccgc
     4381 tgctgccgca gccgtaggcg ccaccggtgg cgccgccgcc cccggccgcc catccggcgc
     4441 gacgccggcc gtgcgagctg cggcgtgcgg tgcggttgaa gctgaccacg gtggccgtga
     4501 gggcgagcac gcccacgacg gtgaggacga cgacgacggt catgagcccg ttccttccag
     4561 gagggcgccc ccgcgacgtc gcggggtgag gcgccagagg tgggagccga ggccgaccac
     4621 gagggtgcgg cgaccggcga gacggacgag ggcgacacgg cgccgtccct cgtgcacgtc
     4681 gtggaggccg agccagtcgt cggccgtcgg cacgtcgacc gagccgtcgc gcagggcggc
     4741 gacgaagcgt cgtacgtcgt cgtgggcgca gccgtcggcg gggtgtgccg cggcccaggc
     4801 gtggaacgcg gtgcgccatc ccggcagctc gtcgagctcc ggtacgacgc ggagcgcgcc
     4861 gtcgctgcgc ttgcggtgca gcacccgcgt ggtgaggccg gctccggccg cgtcgacgcc
     4921 gggcggcacg tccccgcgca ggagcgcggc gagcacctcc tcctggcggg cggccaaccg
     4981 ctcccgggcg gtgccggtgg tcacgcgtgc tcccgcacga gcggcgccgc ggcggcgcgc
     5041 agcacgtcgt actcgctgcg gaccgcggcc tcggtgacgt cgtcgtcgcg ctcgagcagg
     5101 atgccgggca gcgggaggcg gcggtcgtcg taggcggtga cgagcgtgcg cagcagggcc
     5161 agcaccggcg gggtcatcgg gtggccgtgc gtgtcgaggt agaggccgtc ggcgtgctcc
     5221 tcgccgccgg cgacgtgcac gtaggcgacg gcgtcgagcg ggaagcgcag caggtcggcg
     5281 accgggtcgg agccgcgggc ggtggcgctg gcgtgcaggt tggcgacgtc gagcacgagc
     5341 cggcagccgg tggcgtcgac cagggcggtg aggaagtcgg cctcgtcgag ggtgtcctcg
     5401 ggccacgtga agagggcggc gatgttctcg acggcgagcg gcacggggag cgcgtcctgc
     5461 gcggcgcgga cgttctccac gagcacggcg aggctgtcgg cggtgcgcgc gggcggcagc
     5521 aggtggccgg cctcgagcac gtcgccgtgc agcgggtcgg gcgtggtcgc ggcccgcacg
     5581 aacgccacgt gctcgctcac caggggggcg ccgagggcgg tggcgagcgc cgcgaggtgg
     5641 gcgaggcggg cggggtcggg caggtcggcg ccgccgaggc cgagggtgac gccgtggggc
     5701 acgacggtcg cgccggcccc gcggagcgcg aggagggcgg gcggcaggcg gtcggggtgc
     5761 acgttctccg cgacgacctc ggtgaacgac agctcgccgg cggcggcgta cgacgccagc
     5821 aggtcggcga tcggtcggcg ccaggcgacc gcggtgccgc gcaggccgct cacgccgggt
     5881 ccccggaggg cgacgggccc gcggtcacca ccgcgaggac gacggggtcg gcgcaggcac
     5941 cggcgaacgc gtcgatgcgc gcccggatct cgcgaccgag cagccactgg ccgatcggct
     6001 cggcgacgcg gcggagccag gcgggccgga ccgcgtacgt gtacttccac gcggcgaccg
     6061 cgccctcgac cgtgacgccg tcgacctcgc gggcctccgg cgtgaagcgc cagccgccgc
     6121 cgaacgacgc gaagaaccac gggcccttca ccatcgtcat gccgacggag gtgggcgggc
     6181 ggtaggagac gtactcgctg atcatgcgcg gcccgacccg ggcccgggtc agggtgcgca
     6241 cgcccttggc gggccggtcg gcgcccagta gctcctggtg gcggatgaac gggtcccagc
     6301 gcagccgcgt cgcgcccgtc gtctgcgaga cggcgaacgc ctggtcgggg gtgacgggca
     6361 cccaccgcga gacggagacc tgaggcatgc cggcgagtgt tccgtcgccg ggggcaccgc
     6421 aaacgcggcg cgtgggggcg tgcccccgcg cgccgcgacc gggtggtcag ccggcgagcg
     6481 cggcgacctc gtcggcggtg agctcgagcg tcatcgactc gacgagcgcg gtgagctgct
     6541 cggggttgcg ggcgctcgcg atcggggccg agacgaccgg ctgggcgcgc agccacgcga
     6601 gcgccacggc ggtggggctg actccgtgcg cggaggcgat ctcgagctgg gcggcgagca
     6661 cgcggtcggc gccgggcttc tgcgcgtacg acgccgcggt gcccgcgcgg ggcgagtcga
     6721 cctgggcgcc ggcggcgtac ttgccggtga ggaagccgct cgcgagggcc gagtacggcg
     6781 tctcctggat gccgagctcg gcgagggtcg gcacgaggtc ggtctcgatg tcgcgggcca
     6841 ggaggttcca gcggtcctgg ctgacggtga agccggtgac gccctgctcg gcggccagcg
     6901 cgacggcgcc gcgcaggcgc tcggcggaga agttcgaggc gcccaggtgg agcgccttgc
     6961 cctcggccac cagcgcgccg aacgcggcga cgtactcggc ctgcggcacc gactcgtcgt
     7021 cgacgtgggc gtagtagagg tcgacgtggt cggtctggag gcgctccagc gactcgtcga
     7081 gcgcggtgcg gaggttctcc ggcgcgaggc cctcgcggcc gtcgccctgg cccaccttcg
     7141 tggccacgac gacctggtcg cgcacgccgc gggcggtgat ccactcgccg atgatgcgct
     7201 cggagtcgcc gatcgcccgg ccgtccaccc actgcggata catgtcggcc gtgtcgatcg
     7261 agcggccgcc ggcggccagc cacgcgtcga gcacggcgaa cccctggtcg cggtcggcgg
     7321 tccagccgaa cacgttgccg ccgagcatca gcgggccgag gtcgagcgtg gtgggggtgg
     7381 tcgtcgcggg agaggtcacg ggcaggactc ctgggggatc gggggtgcgg tcacctctcc
     7441 caacgctcgc gcccgtcgat gattccccgc tcagagcccg aactgctcgt gccgggcggc
     7501 gggcacgagg tcggcgtact ccgcgttctt ctgcacgaac ttcgtgaaga aggggcagta
     7561 gggcagcagc tggcggccgt cggcgcggag cccgtcgagc acctgcacgg ccagctcctt
     7621 cgcgaggccc tgaccggcga acgactcgcc gatctccgtg tggaggatcg cgacgaccgt
     7681 gccggggccg gcgtcgccgt ccttcagctc gtacgtcgcg aagccggcga ggtcgccgcc
     7741 gacgtggatc tcgaagcggt gctcgtcggt gttctcgacg acggtggggc cgggggcgtc
     7801 ggacggctgc tcggtggtca tggcgcgaat ctaccgctct gacctggccg aacgctcggt
     7861 cggtgggcct cctggtgggc ccgggccgtg acgttcgcct cagatcgggc cgtttcggtc
     7921 gttgcggtcg gaggtgactt gtgcgtaacc cacgacacag cggaccctgg cgggcatgcc
     7981 cccgatcagc ccgcccacgc ccgccaccgc ctcgtcgtac tcgatcacca tgcgtctgca
     8041 cacggcgccc gaccacggga tcgtgggcac ggtggcgacg accatctcca ccgccggggg
     8101 catcgtcacg gcgatcgacg tcgcggactc gcgccacgac cgcctggtgc tcgacgtgac
     8161 gtgctcggcc ggcaacgcgg accactcgga gcagctcgtc gacgccgtcc gggcgctcga
     8221 gggcgtgacg gtgcacaagg tctcggaccg caccttcctg ctgcacatcg gcggcaagat
     8281 cgaggtcacc tcgaaggtgc ccctcaagac gcgtgacgac ctctcgctcg cctacacccc
     8341 gggtgtcggt cgcgtgagcc gcgcgctggc ggagaacccc gaggacgtct cgcgcctgac
     8401 gatcaagggc aactcggtcg ccgtggtcac cgacggctcg gccgtgctgg gcctcggcaa
     8461 catcggcccg ggtgccgcgc tgccggtgat ggagggcaag gccgcgctgt tcaagcgctt
     8521 cgccaacatc gacgcgtggc cgatctgcct ggccacccag gacaccgacg agatcgtcaa
     8581 ggccgtcgag atgatcgccc cgggcttcgg cggcatcaac ctcgaggaca tcgccgcgcc
     8641 gcgctgcttc gagatcgagg cgcgcctccg cgagagcctc gacatccccg tcttccacga
     8701 cgaccagcac ggcaccgcga tcgtcgtgct cgccgcgctc accaacgcgc tgcgctgcgt
     8761 gggcaagcag atggagaccg tgaaggtcgt cgtcgctggc gcgggcgccg cgggcaccgc
     8821 gatcgtcacg ctgctgctgg ccgcgggcgt caccgacgtc gtggtctggg accgcgaggg
     8881 ctgcctctcg ggcgacgaca cgtcgctgcc cccggcgaag ctcgagctcg ccggccgcac
     8941 caacccccgc aaggtcacgg gcacgctgca ggacggcctg cgcggcgccg acgtcttcat
     9001 cggcgtctcg ggcccgggcc tgctccaggc cgagtggatc aaggacatgg ccgacgactc
     9061 gatcgtcttc gcgctggcga acccggaccc cgagatcgac ccggccgagg cgaacaagta
     9121 cgccgcgatc gtggcctcgg gccgctcgga ctacccgaac cagatcaaca acgtgctcgc
     9181 cttcccgggc gtcttccgcg gcctgctcga cgcccgcgcc tccgaggtca ccgtcgaggc
     9241 gctgctgcgc gcggcagagg ccatcgcgct cgtcgtcacc gacgaggagc tcaaccccag
     9301 cttcatcatc ccgtcggtct tcgacgcggc cgtcccgaag gccgtcgccg cggccatcgc
     9361 gggcgaggag cacgccaaga gctgagggtg gagcacccgg agcacgacat cgaggcgctc
     9421 gtcgtcgact gccacccggt cgacggcggg gacctcgcgt gcttccgggt gctcatcctg
     9481 ctgcggcgcg agatcgagca cctggcgccc ggcgccgtcg tctgcctgac gacgacggac
     9541 cccgtggcgc cgatcgacct gccggcctgg tgccggctca ccgggcacga gtacctgggt
     9601 cccgtgcccg ggcggcccac cccgacgtac ggcgtgcgcg tggccgccgc gccgcacgcc
     9661 acccgccccg accggccctg gcaccgggcc tgatcccccc ggtcggggac ctcagccggc
     9721 ctggggcgcg acctcgcgcg gccaccagaa ccgctcgccg agcagcagcg cgatcgccgg
     9781 caccacgacc gtgcgcacga ggagcgtgtc gagcaggacg ccgatgcaga tcacgacgcc
     9841 gagctgcgcg agcaccacca gcggcagaac gccgagcacg gcgaagaccg cggcgagcag
     9901 gatgccggcg ctggtgatca cgccgccggt cgcgccgagc gcccgcagca cgccctcccg
     9961 ggtcccgtgg acggcggcct cctcgcgggt gcgggtgacg aggaagatgt tgtagtcgat
    10021 cccgagcgcc accaggaaga ggaacgcgaa gagcggcacg ttgcccgcga ggctctcgaa
    10081 gcccagcacg ccggtgaaca gccaccacga cgcgcccatg ctggcggcgt acgtcgccac
    10141 caccgtgccc accaggatca ccggcgccac cacggagcgc agcagcacga gcagcacgcc
    10201 cagcacgagc gccaggatga gcggcaggat gacgacccgg tcgcggctgt tgccgtcggc
    10261 ctcgtcgatc gagctggcct ccgcgccgcc gacgaacgtg tcgtcgtacg acgccagcgc
    10321 ctcccgcagc gcgagcaccg cggcgtcggt ctcggcgctg ccgggggtgc cctcgagcac
    10381 gacgtcgagc tgggtgacgc cctcgccctc ggcggtcggc tgcaccgacg ccaccccgtc
    10441 gaccgcctcg accgcggcgg cgacctcgcc cgcgtcggca cgggtcacga cctgggtggg
    10501 ctcggcggtg ccggcgggga aggactcggc gatgcgggcg ctggcgctga tggcctcggg
    10561 ctcgtcgagg aactggtcgg cggtgtcgag accggtcgcg atctgcgtgg tgccgatcgc
    10621 gagggcgccg agcagcacgg cggagccgac cacggccacg accggcttgc gggcgaccgt
    10681 gtcgccgacg cgccgccagc ccgagcggga ctcgaccagc acggtgtcgc cctggtgcgg
    10741 caccttgggc cagaacaccc agcggccgaa gcagacgagg aaggcgggca gcaccagcag
    10801 cacgaacgcg gcggcgacgg cgatgccgac ggcgcaggcg aggccgagcc cgcgggtggt
    10861 cggggtgagc gac
//
