LOCUS       NZ_CP011665            10972 bp    DNA     linear   CON 03-MAR-2024
DEFINITION  Streptomyces sp. Mg1 plasmid pSMg1-1, complete sequence.
ACCESSION   NZ_CP011665
VERSION     NZ_CP011665.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. Mg1
  ORGANISM  Streptomyces sp. Mg1
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 529571)
  AUTHORS   Hoefler,B.C., Konganti,K. and Straight,P.D.
  TITLE     De Novo Assembly of the Streptomyces sp. Strain Mg1 Genome Using
            PacBio Single-Molecule Sequencing
  JOURNAL   Genome Announc 1 (4), e00535-13 (2013)
   PUBMED   23908282
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529571)
  AUTHORS   Hoefler,B.C. and Straight,P.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2015) Biochemistry and Biophysics, Texas A&M
            University, 300 Olsen Blvd., College Station, TX 77845, USA
REFERENCE   3  (bases 1 to 529571)
  AUTHORS   Hoefler,B.C., Konganti,K. and Straight,P.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUN-2013) Biochemistry and Biophysics, Texas A&M
            University, 2128 TAMU, College Station, TX 77843, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 8.2
            Genome Coverage                   :: 30X PacBio; 80X Illumina
            Sequencing Technology             :: PacBio + Illumina MiSeq/HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/03/2024 08:10:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,822
            CDSs (total)                      :: 7,726
            Genes (coding)                    :: 7,467
            CDSs (with protein)               :: 7,467
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 109 of 260
            Pseudo Genes (incomplete)         :: 200 of 260
            Pseudo Genes (internal stop)      :: 18 of 260
            Pseudo Genes (multiple problems)  :: 60 of 260
            CRISPR Arrays                     :: 11
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:43:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 383369
            Orig. end                         :: 394341
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011665.1.
            Bacteria and source DNA available from Paul Straight
            (paul_straight@tamu.edu).
            Annotation modified by the submitter.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10972
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10972
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10972
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5972](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5972)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    216..230
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     misc_feature    complement(1877..1892)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.5"
                     /tool="antismash"
     gene            complement(2455..3513)
                     /locus_tag="M444_RS36355"
                     /old_locus_tag="M444_36340"
     CDS             complement(2455..3513)
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (109-349). E-value:
                     1.6e-19. Score: 62.1. Matches aSDomain:
                     nrpspksdomains_M444_RS36355_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) t2clf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019635009.1"
                     /locus_tag="M444_RS36355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M444_36340"
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="WP_324615578.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 2.2e-12, bitscore:
                     42.1, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="t2clf (E-value: 4.3e-111, bitscore: 366.5,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGLGVAAPNGLGADGFWAATRTGTNGIGRITGFDPSPYPVTLAG
                     QVPGLAAEGRPPSRVLPSRLLAQSGRMPRITLVAAGWALADAGVDPEELGALDLGGLDA
                     GGAAVCAASGLDAVAQARRRIREGAGLVVSGAVDASLSPWGWGVQLVSGRLSGSAEPTR
                     AYLPFDAEAAGHVPGEGGAILILESAAAARARGARVYGEISGYGAVSDPRPGSGREPGL
                     RRAIELALADAGAVPGDVDVVFADAAAIPELDRIEAEAITAVFGPCGVAVTAPKTMTGR
                     LCSGAAPLDLAAALLAIEEGLIPPTVNVRLSDAYQLDLIGPRPRTAEVRTALVLARGHG
                     GFNSAVVVRAVD"
     aSDomain        complement(2467..3186)
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_M444_RS36355_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="1.60E-19"
                     /label="M444_RS36355_PKS_KS.1"
                     /locus_tag="M444_RS36355"
                     /protein_end="349"
                     /protein_start="109"
                     /score="62.1"
                     /tool="antismash"
                     /translation="CAASGLDAVAQARRRIREGAGLVVSGAVDASLSPWGWGVQLVSGR
                     LSGSAEPTRAYLPFDAEAAGHVPGEGGAILILESAAAARARGARVYGEISGYGAVSDPR
                     PGSGREPGLRRAIELALADAGAVPGDVDVVFADAAAIPELDRIEAEAITAVFGPCGVAV
                     TAPKTMTGRLCSGAAPLDLAAALLAIEEGLIPPTVNVRLSDAYQLDLIGPRPRTAEVRT
                     ALVLARGHGGFNSAVVVR"
     CDS_motif       complement(2974..3012)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_M444_RS36355_0001"
                     /evalue="9.00E-03"
                     /label="PKSI-KS_m4"
                     /locus_tag="M444_RS36355"
                     /protein_end="180"
                     /protein_start="167"
                     /score="9.5"
                     /tool="antismash"
                     /translation="FDAEAAGHVPGEG"
     misc_feature    3706..3721
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     gene            complement(3941..4969)
                     /locus_tag="M444_RS36360"
                     /old_locus_tag="M444_36345"
     CDS             complement(3941..4969)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PKS_KR (2-304). E-value: 1.3e-06. Score:
                     20.4. Matches aSDomain:
                     nrpspksdomains_M444_RS36360_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546906.1"
                     /locus_tag="M444_RS36360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M444_36345"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_037792370.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 3.7e-08, bitscore:
                     27.8, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTIMITGAAGFIGGHVAREAERRGGLSLRLMSHRRPPAGAAGAG
                     PATAGPDPATAGPGSGWGHGHGPGAVVPGHRTVRADLSDPGSLRGVCEGVDVLLHCASQ
                     IGGTPEANEAVNARGTAALMAEARRAGVSRVVYLSTASVYGRGAFRAARPEDLVRNPGS
                     PTSGSRARAEDAVLAAGGVVLRPHLVLGDGDRWVVPGLARILRALPGTVEGWAARTSVI
                     TAQDLARLLLGAGLAPAGELTASVYHAAHPVPVTVRTLLGAVAAVAGPAADPAAEDLTA
                     VRARELLAARGIAAQGLDMLTSDHWFDSDPVWRDLRCTPGPDFEAAFKELAEGYRRALL
                     DI"
     aSDomain        complement(4058..4963)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_M444_RS36360_PKS_KR.1"
                     /evalue="1.30E-06"
                     /label="M444_RS36360_PKS_KR.1"
                     /locus_tag="M444_RS36360"
                     /protein_end="304"
                     /protein_start="2"
                     /score="20.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="TIMITGAAGFIGGHVAREAERRGGLSLRLMSHRRPPAGAAGAGPA
                     TAGPDPATAGPGSGWGHGHGPGAVVPGHRTVRADLSDPGSLRGVCEGVDVLLHCASQIG
                     GTPEANEAVNARGTAALMAEARRAGVSRVVYLSTASVYGRGAFRAARPEDLVRNPGSPT
                     SGSRARAEDAVLAAGGVVLRPHLVLGDGDRWVVPGLARILRALPGTVEGWAARTSVITA
                     QDLARLLLGAGLAPAGELTASVYHAAHPVPVTVRTLLGAVAAVAGPAADPAAEDLTAVR
                     ARELLAARGIAAQGLDMLTSD"
     gene            complement(5001..5972)
                     /locus_tag="M444_RS36365"
                     /old_locus_tag="M444_36350"
     CDS             complement(5001..5972)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014627801.1"
                     /locus_tag="M444_RS36365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M444_36350"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_008740559.1"
                     /sec_met_domain="AfsA (E-value: 8.2e-21, bitscore: 69.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSLTALRIPAHHSAPAANCLLTTTVPREYVHRAAVSEVLLTGWG
                     PDPLAGRDQSDSYVVRAQWPRGHSLFAPAGGYQDPLLLIESVRQTGALLAHSEYGVPFG
                     HQFLMWDMSFIATPEALVASPVPTEVELRTVCRDVVRRGRTIASMRYEVSVWRDGVPLA
                     TASAAFSCTSPAVHRRLRGERPTTSDRTPPPAVDPAVVGRSSAPGNVVLAPAPAGPGDR
                     WELRVDTEHPIFFDHPVDHVPGMVLLESARQAAHAVTGLPDALVLGMASTFERYAELDA
                     PCWIEARAERPDAAGDIAVHVVGTQQDRPVFTADLTLRPSAG"
     misc_feature    complement(5694..5696)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(6079..6092)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.76"
                     /tool="antismash"
     misc_feature    6079..6093
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.44"
                     /tool="antismash"
     misc_feature    6296..6311
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.92"
                     /tool="antismash"
     misc_feature    complement(6303..6317)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.58"
                     /tool="antismash"
     gene            6467..7258
                     /locus_tag="M444_RS36370"
                     /old_locus_tag="M444_36355"
     CDS             6467..7258
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018557968.1"
                     /locus_tag="M444_RS36370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M444_36355"
                     /product="response regulator transcription factor"
                     /protein_id="WP_008740558.1"
                     /transl_table=11
                     /translation="MNRQAADVLEPRSEQIRPQRTLLSQPGHFPPPRVERPAAAAEPAL
                     RVLVVENEARAADTLVVGLRRHGYLAESVATGALALQAHGDADLILLDLDLPDLDGLEV
                     CRAIRAVGDTPLIAVTARGSELDRVLGLQAGADDYLVKPYGFRELLARMEAVMRRARPR
                     PQASRVITHGPLRIDAGTREVLLAGRPVELTRKEFDLLHLLASQPEAVISRRQLMTQVW
                     DDTWSHRGRTIDTHVSSLRGKLGASGWIVTVRGVGFRLGRP"
     misc_feature    complement(7358..7368)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            7363..8004
                     /locus_tag="M444_RS36375"
                     /old_locus_tag="M444_36360"
     CDS             7363..8004
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137541.1"
                     /locus_tag="M444_RS36375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M444_36360"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_047961251.1"
                     /transl_table=11
                     /translation="MAQQERGNRSRRSILEAAARVFDERGYDAASTNEILASTGLTRGA
                     LYHHFPSKEAIASALVAAQSEALVVPDQPVKLQAVIDLTLEFARRLQHDPVLRASVRLA
                     VEQTSFSRPAQTPYEQSNATILDLLRQAREQGEVLPGVDLQEATSTIVGAFTGLQVMSQ
                     VYSNRQDLPDRVCALWRFLLPGLATPGMLARLRTAPLPQTAPQAAPQTAP"
     gene            complement(8020..8607)
                     /locus_tag="M444_RS36380"
                     /old_locus_tag="M444_36365"
     CDS             complement(8020..8607)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="M444_RS36380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M444_36365"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_008740556.1"
                     /transl_table=11
                     /translation="MQKRSEQTRQALVSAAARIIADGRPADAGLVNICRTAGVSRGALY
                     HHFSSTTELTAAVYSQAFERATTLAEEAFEGPAESAAARFCSSLCTALRDEELVRAGTQ
                     LAADGSAGPPRLRDDALDLVRRHLVSAGGELAELADLAVVVTAGLESLGRTDGTWWEPR
                     TSERIWDLLRPLFGPRGPARGGSPLGAAGRQD"
     misc_feature    8699..8708
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     misc_feature    complement(8699..8712)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.59"
                     /tool="antismash"
     misc_feature    complement(8778..8791)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.48"
                     /tool="antismash"
     misc_feature    complement(8785..8801)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.19"
                     /tool="antismash"
     gene            complement(8884..10545)
                     /locus_tag="M444_RS36385"
                     /old_locus_tag="M444_36370"
     CDS             complement(8884..10545)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1138: FAD linked oxidase domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030784.1"
                     /locus_tag="M444_RS36385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="M444_36370"
                     /product="FAD-binding protein"
                     /protein_id="WP_008740555.1"
                     /transl_table=11
                     /translation="MANIARRALFRGAAATVSAVAGSAALGAVTAAPAVADEVVGEGQD
                     AMGARTTAPTGATITPNDPRYAQLTTGNNQRYVAAPSYVKMIKSAPDAAAAITAAYRAG
                     KRVSVRSGGHCFSNFVCNPDVQVILDCSEMTYVGYDPAMRAFAVEPGARLLNVYEALYK
                     GWGVTLPGGVCYGVGAGGHIAGGGYGLLSRRNGLVVDHLYAVEVVTVDASGTARIVRAT
                     REPNDPNRDLWWAHTGGGGGNFGVVTRYWLRSPNAPGGSAATPENQLVSPPSHVLMHIQ
                     GIPWSQLDEAKFSRLVKNFGAWHEANSAPNSPLRNLSSILNVYSKASGMLNLVTQVDAG
                     VPDAAAMLDTYLNQILDGVTTARAASRKLPWLQAASLIGTINTTLNDPTLRGAHKSAYM
                     RTNFTDAQLASLYASMTAADYTNPRAMLILFSFGGQVNAVAPEATAIPQRSSAFKMLYQ
                     TFWNSAAEDSTHVAWLRTLYANMHAATGGVPGLDGQTDGCYINYPDTDMADPAQNTSGV
                     PWQRLYYKGNYARLQQVKARWDPSDYFRHSMSVKQV"
ORIGIN
        1 cggtcgactt cacgagcagc cccgacatgc cgatgacgtc ggccttgtgc tgttcggcgg
       61 cttcgaggat cgcggagacg ggctgtttga tgccgatgtt ggcgacgttg tagccgttgt
      121 tggacaggat gatgtcgacg aggttcttgc cgatgtcgtg gacgtcgccg cggacggtgg
      181 cgagcacgat cgtgcccttg ccgtccgcgt cggtcttctc catgtgcggt tcgaggtggg
      241 cgacggcggt cttcatgacc tccgccgact ggaggacgaa ggggagctgc atctggccgg
      301 agccgaagag ctcgccgacg accttcatgc cctccaggag ggtgtcgttg acgatgtcga
      361 gggccgggcg ggtctggagc gcctcgtcga ggtccgcttc caggccgttc ttctccccgt
      421 cgatgatccg gcggcgcagg cgttcctcca ggggcagcgc catcagttcc tcggcgcggc
      481 cggccttcag ggacttggtg ttgacgccct cgaagagggc catcagcttc tggagcgggt
      541 cgtagccctc cgcccgccgg tcgtagatca ggtcgagggc ggtgcccacc tgctcctcgt
      601 cgaagcgggc gatggggagg atcttgctgg cgtggacgat cgcggagtcc aggccggcct
      661 tgacgcactc gtcgaggaag acggagttga ggaggatacg ggcggccggg ttgaggccga
      721 aggagatgtt cgagaggccg agggtggtct ggacgtccgg gtggcggcgc ttgagctcgc
      781 ggatcgcccc gatcgtggcg atgccgtcgc cccgcgactc ctcctggccg gtgcagatgg
      841 tgaaggtgag cgtgtcgatg aggatgtcgg attcgaggat gccccagttc tcggtgaggt
      901 cctggatcag ccgctccgcg atctccacct tgtgctcgac ggtacgggcc tggccctcct
      961 cgtcgatggt cagcgcgatc agcgcggcgc cgtgctcccg cgccagtcgg gtgaccttgg
     1021 cgaagcgcga gtccgggccg tcgccgtcct cgtagttgac ggagttgatg acggcgcggc
     1081 cgccgagctt ctccaggccc gcctggatga cgggaacctc ggtggagtcc aggacgatcg
     1141 gcagcgtcga ggcggtcgcg aaccggccgg ccagttccgc catgtcggcg acgccgtccc
     1201 gcccgacgta gtccacgcac aggtccagca tgtgcgcgcc ctcgctgatc tggtcccgga
     1261 ccatctccac gcagtcgtcc cagcgggcct ccagcatggc ctcgcggaac tgcttcgacc
     1321 cgttggcgtt cgtccgctcg ccgatcgcca tgtacgaggt gtcctgccgg aacggcaccg
     1381 tctggtacag cgaggccgca ccgggctccg ggctcggttc gcgcaccgcc ggggccagcc
     1441 cgcggacccg ctcgacgacc tgccgcaggt gctccggggt cgtgccgcag cagccgccga
     1501 ccagggagag accgtactcg cggacgaagg actcctgggc gtcggcgagc tcgctcgccg
     1561 acagcgggta gtgcgcgccg tccttcgtca gcaccggcag gcccgcgttc ggcatgcagg
     1621 acagcgggat ccgggagttg cgggcgaggt agcgcaggtg ctcgctcatc tccgcggggc
     1681 cggtggcgca gttcagcccg atcatgtcga tgccgagggg ttcgagggcg gtgagggccg
     1741 cgccgatctc ggagcccagc agcatggtgc cggtggtctc gacggtgacc gagcagatca
     1801 gcgggaccga gaggccgagg gcctccatcg cccgccgggc gccgatgacg gaggacttgg
     1861 tctggaggag gtcctgggtg gtctccacca gcagcgcgtc cgcgccgccc gtcaggagcc
     1921 cctccgcgtt cgcctggtag gcgtcgcgga tcgtcgcgta gtcgatgtgg ccgagggtgg
     1981 gcagcttggt gccggggccc atcgagccga ggacccagcg ctgctgcccg gtggcggcgg
     2041 tgaactcgtc cgcggcctca cgggcgatcc gcgcgcccgc ctccgacagc tcgaagttgc
     2101 gctccgcgat gtcgtactcg gcgagggcgg cgaagttcgc gccgaaggta ttggtctcca
     2161 cacagtccac gccgacggag aaatacgcct cgtggaccgt ccggacgata tcggggcggg
     2221 tgacgttcag aatctcgttg cagccttcga ggttctggaa atcatcgagg gtggggtcct
     2281 gcgcctggag catggtgccc atggcgccgt cggcgaccac cacgcgggtc gcgagcgcct
     2341 cacggagggc attggagcgg ttccggctgt caccggaaag ggttggcagc gaggccatgg
     2401 aaaagctcct gttcaacgag agggccgctc aatgtgaaag aacgtcgaat accgtcagtc
     2461 cacggcgcgg accaccacgg ccgagttgaa gccgccgtga ccgcgcgcca ggaccagcgc
     2521 ggtgcgcacc tcggcggtgc gcggccgggg tccgatcagg tccagctggt acgcgtcgga
     2581 cagcctcacg ttgaccgtgg gcgggatcag gccctcctcg atggccagca gcgcggccgc
     2641 caggtcgagc ggggcggcgc ccgaacacag ccggcccgtc atcgtcttgg gcgcggtgac
     2701 cgcgacgccg caggggccga acaccgcggt gatggcctcg gcctcgatcc ggtccagctc
     2761 ggggatggcg gccgcgtcgg cgaagaccac gtccacgtcg ccgggtacgg ctcccgcgtc
     2821 ggccagggcc agctcgatcg cccggcgcag gcccggctcg cgcccgctgc cgggccgggg
     2881 gtcggacacc gctccgtagc cggagatctc cccgtagacg cgggcgccgc gcgcccgggc
     2941 cgccgcggcg gactccagga tcaagatcgc gccgccctca ccggggacgt gtccggcggc
     3001 ctcggcgtcg aaggggaggt aggcgcgggt gggctccgcg ctgccgctca gccggccgct
     3061 gacgagctgg acgccccagc cccaggggct gagcgaggcg tccacggcgc cggagacgac
     3121 caggccggcg ccctcgcgga tgcggcggcg ggcctgcgcg acggcgtcga gcccgctcgc
     3181 cgcgcagact gcggcgccgc cggcgtccag cccgccgagg tcgagcgcgc cgagctcctc
     3241 ggggtccacg cccgcgtcgg cgagcgccca gcctgcggcg acgagcgtga tccggggcat
     3301 ccggccgctc tgcgccagca gccggctcgg cagtacccgg ctcggcggcc gcccctcggc
     3361 ggcgaggccg gggacctggc cggcgagggt caccgggtac ggggaggggt cgaagccggt
     3421 gatccggccg atgccgttcg taccggtgcg ggtcgcggcc cagaagccgt ccgcgccgag
     3481 tccgttgggg gcggcgacgc ccaggccggt cacgaccacc gaggtggccg tgccgcgttc
     3541 ctcccgggac gcggcggccg ggtggcacag ggggatccgg cccggccgtc gccggcatcc
     3601 ggggccgtgg tggaaggccg ggcgcgaacg tccggcgggc cgtaagcggg atcgcgttcc
     3661 gggccgggag tgggcggatt cgcggtcggc gggacgccgc gatccggtga agggtttcac
     3721 ggcaccgatt gccggtgggg ggtccgtccg gaccactgtc agcgtgccgt gcataccttc
     3781 cctctccttt gacccaccgc gaaaagaatg ccggagcacg atgtacagcg tgttccgtcc
     3841 cgggccgcgg ttcaatacgg ggccgtgcag aactcggtca ggtttccgta aggccattgc
     3901 atacgtgtcg ggttcagttc aggagaagtc caggagaatg tcagatgtcg agcagggcgc
     3961 ggcggtagcc ctcggcgagt tccttgaacg cggcctcgaa atccgggccg ggcgtacacc
     4021 gcaggtcccg ccagaccggg tcgctgtcga accagtgatc gctcgtcagc atgtcgagcc
     4081 cctgggccgc gatgccccgg gcggcgagca gctcgcgggc gcgcaccgcc gtgaggtcct
     4141 cggcggccgg gtccgccgcc gggcccgcga cggcggcgac cgcgccgagc agggtgcgga
     4201 cggtgacggg cacggggtgg gcggcgtggt agacggacgc cgtcagctcc ccggccgggg
     4261 cgaggccggc gccgagcagg agccgggcca ggtcctgcgc ggtgatgacg gacgtacggg
     4321 cggcccagcc ctcgacggtg cccggcaggg cccgcaggat ccgggccagg cccgggacca
     4381 cccagcggtc gccgtcgccg aggacgaggt gcgggcgcag gacgaccccg cccgccgcga
     4441 ggacggcgtc ctcggcgcgg gcgcgcgagc ccgaggtggg cgagcccggg ttgcggacga
     4501 ggtcctcggg ccgggcggcg cggaaggcgc cgcggccgta cacggaggcg gtgctgaggt
     4561 agacgacgcg ggagaccccg gcgcgccggg cctcggccat cagggcggcc gtaccgcggg
     4621 cgttgacggc ctcgttggcc tcgggggtgc cgccgatctg cgaggcgcag tgcagcagta
     4681 cgtcgacccc ctcgcagacg ccccgcagcg agccggggtc cgagaggtcg gcgcgcacgg
     4741 tccggtggcc ggggaccacg gcgccggggc cgtggccgtg gccccagcca gagccggggc
     4801 cggccgtggc ggggtcaggg ccggccgtgg cggggccggc ccccgccgcg ccggccggcg
     4861 gtcttcggtg ggacatcagc cgcagggaca ggccgccccg gcgctcggcc tcgcgggcga
     4921 cgtgtccgcc gatgaatccg gccgctcccg tgatcatgat ggtgggcacg cgcgctctcc
     4981 tggtggtggt gatggcgggc tcagcccgcg ctggggcgca gggtgaggtc ggcggtgaag
     5041 accggccggt cctgctgggt gccgacgacg tggacggcga tgtcccccgc cgcgtcgggg
     5101 cgttcggcgc gggcctcgat ccagcagggg gcgtcgagtt cggcgtaccg ctcgaaggtg
     5161 ctcgccatgc cgaggacgag ggcgtccggc agtccggtga cggcgtgggc ggcctggcgg
     5221 gccgactcca gcagcaccat gccggggacg tggtcgacgg ggtggtcgaa gaagatcggg
     5281 tgctcggtgt cgacccggag ctcccaccgg tcgcccggac ccgccggggc gggggcgagg
     5341 acgacgttgc cgggggcgga ggacctgccg accacggccg ggtcgacggc cggcggcggg
     5401 gtgcggtccg aggtggtggg gcgctcgccg cgcagccgcc ggtgcacggc cgggctggtg
     5461 cagctgaagg cggcggaggc ggtggcgagc ggcactccgt cgcgccacac cgagacctcg
     5521 tagcgcatgc tcgcgatggt gcggccgcgg cggacgacgt cgcggcacac ggtgcgcagc
     5581 tcgacctcgg tgggcacggg gctcgccacg agcgcctcgg gggtggctat gaaggacatg
     5641 tcccacatca ggaactggtg cccgaagggg accccgtact cggagtgcgc gagtaaggcc
     5701 ccggtctggc ggaccgactc gatcagcagc agcgggtcct ggtagccgcc ggccggggcg
     5761 aagagggagt ggccgcgcgg ccactgtgcc cgcacgacgt aggagtcgct ctggtcgcgg
     5821 ccggcgagcg ggtcgggtcc ccagccggtc agcagtactt cgctgacggc cgcgcggtgc
     5881 acgtactccc ggggcaccgt ggtggtgagc aggcagttcg ccgccggagc gctgtggtgg
     5941 gcggggatac ggagagcggt gagggacgac atgcgctgta ctcctgttct gcttgaagag
     6001 caggggtgag ccacgcacac gtcggcagcc ccccgctgtg atcgcttgca gacctctaag
     6061 atacagacag gtcggttctt ttcttaggaa atcttcgagt tttccgtaac aaccgctgac
     6121 ccggaccgac ggaatcccgc caagaggtcg gttcctcccg cgcgagcgcc gccggaaccc
     6181 ttgactccgg ccccaactcc actggaacag acagcactgc cgggtttacg cgcccgcaac
     6241 gcgggtcgcc gcacctctcc tccgatcatg gaaaacgacc aaactggcag cccaaaccac
     6301 cctattttca cgatacttgc tgttcagaga ggcctcccgg cgacccgacc aacaccaacg
     6361 cgcggagccc gcactcccgt tgacaatcag acaagtctgt ttggtatctt cattcagcac
     6421 gagcgttcag cagtccgatg gcgcgactgc gcctcaggag gagcagatga atcggcaggc
     6481 cgcggatgtc ctcgaacccc gctcggagca gatccgcccc cagcgcaccc tgctttccca
     6541 gccgggccac ttcccgccgc cgcgggtgga gcggcccgcc gccgcggccg aaccggccct
     6601 gcgggtcctc gtcgtcgaga acgaggcgcg cgcggccgac accctggtgg tggggctgcg
     6661 ccggcacggc tacctggccg agagcgtggc caccggcgcc ctcgccctcc aggcgcacgg
     6721 cgacgccgac ctgatcctcc tcgacctcga cctgcccgac ctcgacggcc tggaggtctg
     6781 ccgcgccatc cgcgccgtcg gcgacacccc gctgatcgcg gtcaccgcgc gcggcagcga
     6841 gctcgaccgg gtgctcgggc tccaggccgg ggcggacgac tacctcgtca agccgtacgg
     6901 tttccgcgaa ctgctcgcgc ggatggaggc ggtgatgcgc cgggcccgcc cccggccgca
     6961 ggccagccgg gtcatcacgc acgggccgct gcgcatcgac gccggcaccc gcgaggtcct
     7021 gctggccggc cggccggtgg agctgacgcg caaggagttc gacctgctcc acctgctcgc
     7081 ctcgcagccg gaggcggtca tctcccgccg ccagctgatg acgcaggtgt gggacgacac
     7141 ctggtcgcac cgcggccgca ccatcgacac ccacgtcagc agcctgcgcg gcaagctcgg
     7201 ggcgagcggc tggatcgtca ccgtgcgcgg cgtcgggttc cgcctcggcc ggccgtagcc
     7261 cctcccgggc gcccgggagg ggccgcccgg gcccctcgtt ccgcagaccg atacgattcc
     7321 gacaggtcgg tttggttcca gtcagtaacg caaggacgtg gcatggcaca gcaagaacgc
     7381 ggcaaccggt cgaggcggtc gatcctggag gccgccgcgc gcgtcttcga cgagcgcggg
     7441 tacgacgccg cgagcacgaa cgagatcctc gcgagcaccg gtctgaccag gggggcgctc
     7501 taccaccact tcccctcgaa ggaggcgatc gcctccgcgc tggtggccgc gcagagcgag
     7561 gcgctggtgg tgcccgacca gcccgtcaag ctccaggccg tcatcgacct cacgctggag
     7621 ttcgcgcgcc ggctccagca cgacccggtg ctgcgggcga gcgtacggct ggcggtggag
     7681 cagacctcct tctcgcgccc ggcgcagacc ccgtacgagc agtcgaacgc gaccatcctc
     7741 gacctgctgc gccaggcgcg ggaacagggt gaggtgctgc ccggcgtgga cctccaggag
     7801 gccacgtcca cgatcgtggg ggccttcacc ggcctccagg tgatgtcgca ggtgtacagc
     7861 aaccggcagg acctgccgga ccgggtctgc gcgctgtggc ggttcctgct gcccggactg
     7921 gccacgcccg gcatgctcgc ccggctgcgc acggcgccgc tgccgcagac cgcaccgcaa
     7981 gccgcgccgc agaccgcgcc gtagcccccg cgcgccccgt cagtcctgac ggcccgccgc
     8041 ccccaggggg cttcctcccc gcgccggccc ccgggggccg aacagggggc gcagcaggtc
     8101 ccagatccgc tccgaggtgc gcggttccca ccacgtgccg tcggtgcgcc ccagcgactc
     8161 cagcccggcg gtgaccacca cggcgaggtc ggccagttcg gccagttccc cgccggccga
     8221 gaccaggtga cggcggacca ggtccagggc gtcgtcccgc agccgcggcg gcccggcgct
     8281 gccgtccgcc gccagctgcg tgcccgcccg tacgagttcc tcgtcgcgca gcgcggtgca
     8341 cagcgaggaa cagaaccggg cggcggccga ctcggcgggg ccttcgaagg cctcctccgc
     8401 cagggtcgtg gcccgctcga aggcctggct gtacacggcg gcggtcagct ccgtggtgga
     8461 ggagaagtgg tggtagagcg ctccgcggct cactccggcg gtgcggcaga tgttcaccag
     8521 cccggcgtcg gcgggccggc cgtccgcgat gatcctcgcc gcggcggaga ccagggcctg
     8581 gcgggtctgt tcggaacgct tctgcatggc gcggcacctg catctctctc ttcacggcac
     8641 acagaagcgc gggggaccgc cgggccgtcg gcactacacc gaatcataca accctgcctg
     8701 ttttttgaaa cccctcgccc aacgagagcc cctttacagc ccctttgacc tccccggatg
     8761 cttggtattc gctgatttcg cgcagagtga ttcagtaaca accagacaag tctgttcgtt
     8821 atgacggcgt cggccggcgc cgtgaccttc cctcaccggc cgacgcggga gcactccggc
     8881 cggtcagacc tgcttcacgg acatcgagtg ccggaagtag tcggaggggt cccacctggc
     8941 cttgacctgc tgcagccgcg cgtagttgcc cttgtagtac agccgctgcc acggcacgcc
     9001 ggaggtgttc tgcgccggat cggccatgtc ggtgtccggg tagttgatgt agcagccgtc
     9061 ggtctggccg tcgaggcccg ggaccccgcc cgtcgccgcg tgcatgttcg cgtacagggt
     9121 gcgcagccag gcgacgtgcg tgctgtcctc ggccgcgctg ttccagaacg tctggtagag
     9181 catcttgaag gccgagctgc gctgcgggat cgccgtggcc tcgggcgcga ccgcgttcac
     9241 ctggccgccg aaggagaaca ggatcagcat ggcgcgcggg ttcgtgtagt ccgccgccgt
     9301 catcgacgcg tacagcgagg cgagctgcgc gtccgtgaag ttggtgcgca tgtacgccga
     9361 cttgtgggcc ccgcgcagcg tggggtcgtt gagcgtggtg ttgatggtgc cgatgaggct
     9421 ggcggcctgg agccacggca gtttgcggct cgcggcgcgc gcggtggtga ccccgtcgag
     9481 gatctggttg aggtaggtgt cgagcatcgc ggccgcgtcg gggacgccgg cgtccacctg
     9541 ggtgaccagg ttcagcatcc cgctggcctt ggagtagacg ttcaggatgc tgctcaggtt
     9601 gcgcagcggc gagttggggg cgctgttcgc ctcgtgccag gcgccgaagt tcttcacgag
     9661 ccggctgaac ttggcctcgt ccagctggct ccagggaatg ccctggatgt gcatcaggac
     9721 gtgggagggc ggggagacga gctggttctc cggggtggcc gcggagccgc cgggggcgtt
     9781 cggggagcgc agccagtagc gcgtgaccac gccgaagttc ccgccgccgc cgcccgtgtg
     9841 cgcccaccac aggtcccggt tggggtcgtt cggctcgcgg gtggcccgca cgatgcgggc
     9901 ggtgccggag gcgtcgacgg tgacgacctc gacggcgtag aggtggtcga cgaccaggcc
     9961 gttgcggcgg gagaggagac cgtagccgcc gccggcgatg tggccgcccg cgccgacgcc
    10021 gtagcagacg ccgccgggga gggtgacacc ccagcccttg tacagggcct cgtagacgtt
    10081 gaggagacgg gcgccgggct cgacggcgaa ggcccgcatg gccgggtcgt agcccacgta
    10141 cgtcatctcg gagcagtcga ggatgacctg gacgtccggg ttgcagacga agttgctgaa
    10201 gcagtggccg ccgctgcgca ccgagacccg cttgccggcc cggtacgcgg cggtgatggc
    10261 ggcggccgcg tcgggcgccg acttgatcat cttcacgtac gagggcgcgg cgacgtaccg
    10321 ctggttgttg ccggtggtga gctgcgcgta ccgcgggtcg ttcggggtga tcgtggcccc
    10381 ggtcggcgcc gtcgtacgcg ctcccatggc gtcctggccc tcgcccacga cctcgtcggc
    10441 gacggccggg gcggcggtga cggcgccgag cgccgcgctg ccggcgacgg cggagacggt
    10501 ggcggcggcg cctctgaaga gcgcccgtct ggcgatgttg gccatgctgg tttccccctt
    10561 ggatgtgacg gtgtgtggga ggggcccccg gcggctgccg ggggcccgtc cggggtggga
    10621 gtcggatgcg ggacgtcggg aacgggaagg gaccgccggg gcccccggtg gccgggggcc
    10681 ggggcggcta gcccttgggg gccgggccgt tgcccagcga cgaggcgccg ctggcgtcga
    10741 tgacctggcc ggagacccag ccggcctcgc cggacgcgag gaacgcggcg atgcccgcca
    10801 cgtccgccgg ggtgccggtg cggccgaacg ccgaccagct ctcggccgcg gcccgcatct
    10861 gcgggtacgc cagccagggg ttgaagtcgg tgtcgatgat gccgggggcg atcgcgttga
    10921 cggtgatccc gcggggcgcg agcgcctcgg cgagggtcag ggtcagggtg tt
//
