LOCUS       NC_021252              10390 bp    DNA     circular CON 28-MAR-2024
DEFINITION  Amycolatopsis keratiniphila, complete sequence.
ACCESSION   NC_021252
VERSION     NC_021252.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis keratiniphila
  ORGANISM  Amycolatopsis keratiniphila
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis; Amycolatopsis japonica group.
REFERENCE   1  (bases 1 to 8948591)
  AUTHORS   Tang,B., Wang,Q., Yang,M., Xie,F., Zhu,Y., Zhuo,Y., Wang,S., Gao,H.,
            Ding,X., Zhang,L., Zhao,G. and Zheng,H.
  TITLE     ContigScape: a Cytoscape plugin facilitating microbial genome gap
            closing
  JOURNAL   BMC Genomics 14, 289 (2013)
   PUBMED   23627759
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8948591)
  AUTHORS   Xu,L., Zhao,W., Ge,M. and Zhao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2012) Shanghai Laiyi Center for Biopharmaceuticals
            R&D, Shanghai 201203, China
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_000400635.2
            Current Name                      :: Amycolatopsis keratiniphila
            Previous Name :: Amycolatopsis orientalis HCCB10007
            Date Updated                      :: 2018-10-26
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_001620365.2
            A1 Name                           :: Amycolatopsis keratiniphila
            A1 ANI                            :: 96.94781%
            A1 Query Coverage                 :: 84.86%
            A1 Subject Coverage               :: 83.93%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_000478275.1
            A2 Name :: Amycolatopsis orientalis HCCB10007
            A2 ANI                            :: 90.22668%
            A2 Query Coverage                 :: 73.73%
            A2 Subject Coverage               :: 73.08%
            ##Taxonomic-Update-Statistics-END##
            ##Assembly-Data-START##
            Assembly Method                   :: Newbler v. v2.5
            Sequencing Technology :: Sanger dideoxy sequencing; 454; Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000400635.2-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 02:04:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,307
            CDSs (total)                      :: 8,242
            Genes (coding)                    :: 8,193
            CDSs (with protein)               :: 8,193
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 14 of 49
            Pseudo Genes (incomplete)         :: 33 of 49
            Pseudo Genes (internal stop)      :: 3 of 49
            Pseudo Genes (multiple problems)  :: 1 of 49
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:56:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8181788
            Orig. end                         :: 8192178
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003410.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from Shanghai Laiyi Center for
            Biopharmaceuticals R&D, Shanghai 201203, P.R. China,
            hccbred@gmail.com.
            The organism was changed from 'Amycolatopsis orientalis HCCB10007'
            to 'Amycolatopsis keratiniphila' in October 2018.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10390
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="29"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10390
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10390
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5390](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5390)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    92..110
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.56"
                     /tool="antismash"
     gene            complement(544..3333)
                     /locus_tag="AORI_RS77910"
                     /old_locus_tag="AORI_7379"
     CDS             complement(544..3333)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631926.1"
                     /locus_tag="AORI_RS77910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_7379"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_016337644.1"
                     /transl_table=11
                     /translation="MAESGSPARFQLLGPVRLLDGDRPVPVGGPGVRGLLALLALKVNK
                     VVALDEIIDALWGHDPPATARTIVHGNVSHLRRVLRDIQGDHPRGARILTAPPGYQLTV
                     EPDRIDVHRARSLLERASAEEPDKASELLAEALSLWQGPALAGVPGSVRAPELEDLRLA
                     VHGARVDADLELGRHAELIVELSPLVRAAPLAERTAGQLMRALYHAGRRGDALELYRTV
                     SRATLRTLGVEPGADLRWLHERVLNDDLPVKVVAEPKPETAPVQQLPPAVPNLAGREAD
                     LAWLDELAERAEAGETAVGVVTGTAGIGKSSLVVWWAHRAARRFPDGILFAALRGFDPQ
                     HPPLEPAELLTQFLLGLGVPAEGVPEQVHERVALYRSMIAGRRMLVILDNARSAEQVRP
                     LLPPGSRSMALVTSRSRLDGLAVSNAAKLRVLGTLAPGDAVRLIEELAGPAGYDLNHAL
                     ARLCGYLPLALRIAGARLAASAQWSAQDLVDELGNERTRLAALDVEGPDDGVRAAFDVS
                     FRGLPSEVADTFLRLGVVQCVSAGSHLTAAIGGITVAEARRHLRVLAAHNLLAETGRDA
                     FVPHDLVRLFLRELAENELDEADREAVLSRSVRFYQAVGDRARRKMLRIVDPLDFTGVL
                     TDAQTPPIGSFDDAQDWFTSEWENLLEVLEAARAAGRHDDVWRLARVAHTYRAVYPLLD
                     EWTRLVEIGLEAAERSGDVLGRCWMLISRCAIALTFELPQGCLADAERALELASAIGDS
                     RLMVSANIHLGSALTLLGRYDEAIRTLRQAVEETERTGDLELRGQALNNCAEAEKRAGR
                     FIEAIGHQVASLEIDRTLGDDSYVVVSLNNLAELSMRIGELAAAERYVWEAVDLTISRR
                     FVLQEGVLRLTLGRVLRAGSDVDGAREQFALALKILADANPKLAGLVRAELTDLGESP"
     misc_feature    3525..3534
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
     misc_feature    complement(4064..4078)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.29"
                     /tool="antismash"
     gene            complement(4077..4976)
                     /gene="thpD"
                     /locus_tag="AORI_RS77915"
                     /old_locus_tag="AORI_7380"
     CDS             complement(4077..4976)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="AORI_RS77915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_7380"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_016337645.1"
                     /sec_met_domain="PhyH (E-value: 1.1e-31, bitscore: 109.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTDTRVDDSYPTRITGKPEHLPRTHPTVWGTEADGPLDAATLA
                     NHEARGYTVVDQLLSPGEVQTYWQELVRMSSGDHRDDERVITEAKTGEVRSIFDVHETS
                     ELIAELVRDPRVLDRARQILGSEVYIHQSRVNYMPGFKGTGFYWHSDFETWHAEDGMPV
                     PRAVSCSIALTDNYPFNGGLMVMPGSQRTFVQCAGETPEDNYKSSLKEQRVGVPSEEDI
                     TALAAEYGIDQFTGQAGSALWFDSNVMHGSSNNITPYPRSNIFVVFNSVENALREPYAA
                     IEPRPAFIAGRDSTPLTR"
     gene            complement(5001..5390)
                     /locus_tag="AORI_RS77920"
                     /old_locus_tag="AORI_7381"
     CDS             complement(5001..5390)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165143.1"
                     /locus_tag="AORI_RS77920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_7381"
                     /product="ectoine synthase"
                     /protein_id="WP_007033454.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.1e-53, bitscore:
                     179.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVRTLDEVTDTDADIKTPNWRSKRIVLAKEGVGFSVHETTLYAG
                     TVNDFWYANHIEAVFITSGEGELENTATGEVFQLKPGTLYLLNDHDKHQVRPKTEIKCV
                     CVFNPPITGREVHDENGVYPLVTEP"
     gene            complement(5435..6685)
                     /gene="ectB"
                     /locus_tag="AORI_RS77925"
                     /old_locus_tag="AORI_7382"
     CDS             complement(5435..6685)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 3.4e-73.
                     Score: 238.5. Matches aSDomain:
                     nrpspksdomains_AORI_RS77925_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631928.1"
                     /locus_tag="AORI_RS77925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_7382"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_043848424.1"
                     /sec_met_domain="Aminotran_3 (E-value: 8.3e-75, bitscore:
                     250.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFEELESEVRSYSRGWPVVFDRAQGSYLYDEDGKAYLDFFAGA
                     GALNYGHNNPALKRALIDYIARDGVTHALDMFTVAKRDFLQTFQEKILQPRDLDYKVVF
                     PGPGGANAVEAALKLARKVTGKESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHA
                     TPMPYDNYFDGSIPDFLYFEKLLEDSGSGLNEPAAVIVEGVQGEGGINAARIEWLKALD
                     DLCKKHGILLILDDVQMGCGRTGPFFSFEDAGIKPDIVCLSKSIGGYGIPMALTLIRPD
                     LDVWEPGEHNGTFRGISPAFVTATEALRVYWSDDELEKSTKAKGERIAAAFQGIVEAYP
                     DANLFAKGRGLARGIEFANGDLAGKVCAAAFERGLLMETSGPDGEVMKVLPPLTLTDDE
                     LTKGLSIIDEAVAAVLN"
     aSDomain        complement(5612..6622)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AORI_RS77925_Aminotran_3.1"
                     /evalue="3.40E-73"
                     /label="AORI_RS77925_Aminotran_3.1"
                     /locus_tag="AORI_RS77925"
                     /protein_end="358"
                     /protein_start="21"
                     /score="238.5"
                     /tool="antismash"
                     /translation="FDRAQGSYLYDEDGKAYLDFFAGAGALNYGHNNPALKRALIDYIA
                     RDGVTHALDMFTVAKRDFLQTFQEKILQPRDLDYKVVFPGPGGANAVEAALKLARKVTG
                     KESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHATPMPYDNYFDGSIPDFLYFEK
                     LLEDSGSGLNEPAAVIVEGVQGEGGINAARIEWLKALDDLCKKHGILLILDDVQMGCGR
                     TGPFFSFEDAGIKPDIVCLSKSIGGYGIPMALTLIRPDLDVWEPGEHNGTFRGISPAFV
                     TATEALRVYWSDDELEKSTKAKGERIAAAFQGIVEAYPDANLFAKGRGLARGIEFA"
     misc_feature    6716..6731
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.5"
                     /tool="antismash"
     gene            complement(6724..7212)
                     /gene="ectA"
                     /locus_tag="AORI_RS77930"
                     /old_locus_tag="AORI_7383"
     CDS             complement(6724..7212)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664377.1"
                     /locus_tag="AORI_RS77930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_7383"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_016337647.1"
                     /transl_table=11
                     /translation="MSGKHLIETPTKADGAALWRIARDSKKLDLNTPYAYLLWCHDFAD
                     TSVVAKVDGVPVGFVIGYRKQDTGFVWQVAVDASQRGKGLAGALLDELFGRLTAQGVRY
                     LETTITPDNEASIRLFASFAKRWDATVERQDLFSAADFPAEEGTHEQEDLYRIGPLSQ"
     misc_feature    complement(8053..8071)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.86"
                     /tool="antismash"
     misc_feature    complement(10149..10163)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.02"
                     /tool="antismash"
     misc_feature    10150..10164
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.74"
                     /tool="antismash"
ORIGIN
        1 gccaccggcg agtcgggctt gcacatcccg gccgtcgccg gggacaagaa caccgtcgag
       61 ttcgtcgtca gcgcctcggg cggcgcgacc atccggttcg gctcgctcaa cgaccagcgg
      121 accgagacga ccccggccag taccgacgaa tggcgcggca cggcgtcctt caactcgggc
      181 gactacctgc tcaccgtcac cgcggacacc cggaactcgt cggtcagcaa ccagatcgcc
      241 tgcacgatcc tggtcaacgg ccagaaggtc gcggagaaca aggggcagac catcgccctg
      301 tgcaccgccg gcacccgctg accccacgcg tttcgtggtg ccgcctggcg gccggccggt
      361 atcgccccca cctccaccgg ccggaccgcc acgagccgct cctggcggct cttcgggcgg
      421 gcccgcgaag gcccgcccga gcgccgatcc ctatcgcatg cccctcccct agggctcgat
      481 cgacgctgaa acggacccta cgcgcgtgag cggcgaggcc actaagaatc cgctaaggat
      541 cactcacgga ctttcgccga ggtccgtgag ttccgcacgg accaatcccg cgagtttcgg
      601 attggcgtcc gcgaggatct tcagcgcgag cgcgaattgc tcgcgggcac cgtccacatc
      661 cgacccggcc cgcagcaccc ggcccaaggt cagccgcagg acgccctcct ggaggacgaa
      721 ccgcctgctg atcgtcaggt ccacggcctc ccagacgtat cgctcggccg cggcgagttc
      781 gccgatccgc atgctcaatt cggcgagatt gttcagcgag acgacgacgt agctgtcgtc
      841 gccgagggtc cggtcgattt ccagcgaagc gacctgatgc ccgatcgcct cgatgaaccg
      901 accggcccgt ttttcggcct cggcgcagtt gttgagcgcc tgcccgcgca gttcgaggtc
      961 gccggtgcgc tcggtctcct cgaccgcctg ccgcaacgtc ctgatcgcct cgtcgtaccg
     1021 gccgagcagg gtcagcgcgg agccgaggtg gatgttcgcc gagaccatca gcctgctgtc
     1081 gccgatcgcg ctcgcgagtt ccagcgcgcg ctcggcgtcg gcgaggcagc cctgaggcag
     1141 ctcgaaggtg agcgcgatgg cgcaccggga gatcagcatc cagcaccggc cgagcacgtc
     1201 gccggaccgt tcggcggcct ccagcccgat ctcgaccagc ctcgtccatt cgtccagcag
     1261 cgggtacacc gcgcgatagg tgtgcgcgac cctggccagc cgccagacgt cgtcgtgccg
     1321 tccggcggcg cgggcggctt cgaggacttc gagcaggttc tcccattccg aggtgaacca
     1381 gtcctgcgcg tcgtcgaagg aaccgatcgg cggcgtctgc gcgtcggtca gcacgccggt
     1441 gaaatcgagg gggtcgacga tccggagcat cttgcgccgg gcccggtcgc cgacggcctg
     1501 atagaaccgc accgaacgcg acagcacggc ctcccggtcg gcctcgtcga gttcgttctc
     1561 cgccagttca cggaggaaca gccggaccag atcgtgcggg acgaaggcat cgcggccggt
     1621 ttcggcgagc aggttgtgcg cggcgagcac ccgcagatgc cgccgtgcct cggcgacggt
     1681 gatcccgccg atcgcggccg tcagatgcga accggccgaa acgcactgga cgacgccgag
     1741 ccgcaggaag gtgtccgcga cctcggacgg caggccgcgg aacgagacgt cgaacgccgc
     1801 gcggaccccg tcgtccggcc cttcgacgtc gagcgcggcc agcctggtgc gctcgttgcc
     1861 gagctcgtcg accagatcct gcgcggacca ctgcgcgctg gcggcgagcc gggcccccgc
     1921 gatccgcagc gccagcggga ggtagccgca cagccgggcg agcgcgtggt tgaggtcgta
     1981 gcctgccggc ccggcgagct cctcgatcag ccggacggcg tcgccgggcg cgagcgtgcc
     2041 gagcacccgg agtttggcgg cgttggacac ggcgagcccg tcgaggcggg aacggctggt
     2101 caccaaggcc atcgaccgcg aaccgggcgg cagcaacggc cggacctgct cggcggagcg
     2161 ggcgttgtcg aggatcacca gcatccggcg gccggcgatc atcgagcggt agagggcgac
     2221 gcgttcgtgg acttgttccg ggacgccctc ggccgggaca ccgagcccga gcaggaactg
     2281 ggtgagcagt tccgcgggct ccaacggcgg atgctgcggg tcgaaaccgc gcagcgcggc
     2341 gaaaaggatc ccgtcgggga agcggcgggc cgcccgatgc gcccaccaca ccaccaggct
     2401 gctcttgccg atcccggcgg tgccggtgac cacgccgacc gcggtctcgc ccgcttcggc
     2461 ccgctcggcg agttcgtcga gccaggccag atccgcctcg cggccggcga ggttcggcac
     2521 cgcgggcggc agctgctgga cgggcgccgt ctcgggcttc ggttccgcga cgaccttgac
     2581 cggcagatcg tcgttgagga cgcgttcgtg cagccagcgc aggtcggcgc cgggttcgac
     2641 gccgagcgtg cgcagggtcg cccgggacac cgtgcggtag agctcgagcg cgtcgccgcg
     2701 gcgcccggcg tggtacagcg cgcgcatcag ctgaccggcg gtgcgctcgg cgagcggcgc
     2761 ggcccggacc agcgggctga gctcgacgat cagttccgcg tgcctgccga gttcgagatc
     2821 cgcgtcgacc ctggcgccgt ggacggcgag ccgcagatcc tccagttcgg gggcgcggac
     2881 cgaacccggc acaccggcca gcgcgggccc ctgccagagc gagagggcct ccgcgagcag
     2941 ttcggacgcc ttgtccggct cctccgccga agcccgctcc agcagggacc gggcacggtg
     3001 gacgtcgatc cggtcgggct cgaccgtgag ctggtagccg ggcggcgcgg tgaggatgcg
     3061 ggcgccgcgc gggtgatcgc cctggatgtc gcggagcacg cggcgcagat gcgaaacgtt
     3121 gccgtggacg atcgtgcggg cggtcgcggg cggatcgtga ccccagagcg cgtcgatgat
     3181 ctcgtcgagc gcgacgacct tgttgacctt cagcgccagc agcgcgagca ggccgcggac
     3241 gccgggtccg ccgacgggga ccggccggtc gccgtcgagg agacgcacgg gaccgaggag
     3301 ttggaagcgg gcaggggaac cgctttcggc catgacaagc ccccctccct ccgatctctc
     3361 cccttaccgg agtagccaac ctaccgcgcg aagggcccgc caaccagaac gcggcaggtc
     3421 gtgaaggcct ccttccctac ttcgagggta gggaaggagg ccttcacgaa tgctcgatac
     3481 ggacaaaagc cccacgggag tctctgtcca ctaaggacct ctgggaaggc ggcgctcatg
     3541 tcctgaaggc ctcctttagg acgctgaacg tctcaaagga ggccttcagg acatgagcga
     3601 tcagcgccag gggctcgcct gaagtgcgtg aaggccccct tcctgcacct aggctcaagg
     3661 aaggggacct tcatgtacct ggcaggcgct gtggctgctg gtgccgatgg ggcatggctg
     3721 gccgcaagtc cgtgaaggcc tccttgaggg actctgggtc cctcaaggag gccttcacgg
     3781 aacgagcctg ggcccacgga gacataaaaa ggctccgtgc catcagtccc gatctgatgg
     3841 cacggagccg ggggatgtcc gacttgggcc gcctgggggc gcgacccaca tcggacatcg
     3901 gcgtccacgt ccgtccgggt gggctcaccc cgagcgaaga acccgttcgg tcgtcgacaa
     3961 cacctacgtg agcgagccca ctggcccgct ctcgtggcga caacgtaaca gggcgcacac
     4021 cgatggctac aagcgccccg tcagggcctt cgcgacctgc ggtttcgcaa atcgggtcag
     4081 cgcgtcagcg gggtcgagtc gcggccggcg atgaaggcgg gacgcggttc gatcgcggcg
     4141 tacggctccc gcaacgcgtt ctcgacgctg ttgaacacga cgaagatgtt cgacctcggg
     4201 tacggcgtga tgttgttgga cgaaccgtgc atcacgttcg agtcgaacca gagcgcggaa
     4261 ccggcctgcc cggtgaactg gtcgatcccg tattcggcgg cgagcgcggt gatgtcctcc
     4321 tcgctcggga cgccgacccg ctgctctttc agcgaactct tgtagttgtc ttccggggtt
     4381 tccccggcgc actgcacgaa ggtccgctgc gaacccggca tcaccatcag cccgccgttg
     4441 aacgggtagt tgtcggtgag cgcgatggag cagctgaccg cgcggggcac gggcataccg
     4501 tcctcggcgt gccaggtctc gaagtccgag tgccagtaga acccggtgcc cttgaagccg
     4561 ggcatgtagt tcacgcggct ctggtggatg tagacctcgg agccgaggat ctgccgggcc
     4621 cggtcgagca cgcgcgggtc gcggacgagt tccgcgatca gctcgctggt ctcgtggacg
     4681 tcgaagatgg agcggacctc accggtcttg gcctcggtga tgacgcgctc gtcgtcgcgg
     4741 tggtcccccg acgacatcct gacgagttcc tgccagtagg tctggacctc gcccggcgac
     4801 agcaactggt cgacgacggt gtaaccacgc gcttcgtggt tggcgagcgt ggccgcgtcc
     4861 aagggaccgt cggcctcggt gccccagacg gtggggtgcg tcctgggcag gtgttcgggc
     4921 ttacccgtga tgcgggtcgg gtaactgtcg tcgactcggg tgtcggtcag cgtcaatgca
     4981 gtcgcctcct cgagcgattt ctagggttcg gtgacgagcg ggtacacgcc gttttcgtcg
     5041 tggacctcac ggccggtgat gggcgggttg aacacgcaga cgcacttgat ctcggtcttg
     5101 ggccggacct ggtgcttgtc gtggtcgttg agcaggtaca acgtgcccgg cttcagctgg
     5161 aagacctcac cggtcgcggt gttttcgagc tcgccttcac cggaggtgat gaacaccgct
     5221 tcgatgtggt tcgcgtacca gaagtcgttg accgtcccgg cgtacagcgt ggtctcgtgc
     5281 accgagaatc cgacgccttc cttggccagc acgatgcgct tgctgcgcca gttcggggtc
     5341 ttgatgtcgg cgtcggtgtc ggtgacctcg tcgagggttc tgacaagcaa cggaaaactc
     5401 cttctcgttc ctgattgagg gcggtcggtt cgggtcagtt gaggacggcg gcgacggcct
     5461 cgtcgatgat cgacaggccc ttcgtgagct cgtcgtccgt cagggtgagc ggcggcagga
     5521 ccttcatgac ctcgccgtcg gggccggagg tctccatcag cagcccgcgc tcgaacgcgg
     5581 cggcgcagac cttgcccgcg aggtcgccgt tggcgaactc gatgccccgg gccaggccgc
     5641 ggcccttcgc gaacaggttc gcgtccgggt acgcctccac gatgccctgg aaggcggcgg
     5701 cgatgcgctc gcccttcgcc ttcgtcgact tctccagctc gtcgtcgctc cagtagacgc
     5761 gcagcgcctc ggtcgcggtg acgaacgccg ggctgatgcc gcggaaggtg ccgttgtgct
     5821 cacccggctc ccagacgtcg aggtccgggc ggatcagcgt cagcgccatc gggatgccgt
     5881 agccgccgat ggacttcgag aggcagacga tgtcgggttt gatgcccgcg tcctcgaagc
     5941 tgaagaacgg gccggtgcgg ccgcagccca tctggacgtc gtcgaggatc agcaggatgc
     6001 cgtgcttctt gcacaggtcg tccagcgcct tgagccactc gatacgggcg gcgttgatgc
     6061 cgccttcgcc ctgcacgcct tcgacgatca cggcggcggg ctcgttcagg ccgctgccgg
     6121 agtcttcgag cagcttctcg aagtagagga agtccgggat ggagccgtcg aagtagttgt
     6181 cgtacggcat cggggtggcg tgcaccagcg ggacgcccgc gccgccgcgc ttcatcgagt
     6241 tgccggtcac cgagagggcg cccagcgtca tcccgtggaa ggcgttggtg aagttgatga
     6301 ccgactcctt gccggtcacc ttgcgggcga gcttcagcgc ggcctcgacg gcgttcgcgc
     6361 caccggggcc ggggaacacg accttgtagt cgagatcgcg cggctggagg atcttctcct
     6421 ggaaggtctg gagaaaatcc cgtttcgcca cggtgaacat gtcgagcgcg tgcgtcacgc
     6481 cgtcacgggc gatgtagtcg atcagcgccc gcttcagcgc cgggttgttg tgcccgtagt
     6541 tgagggcgcc ggcccccgcg aagaagtcga gataggcctt gccgtcttcg tcgtacaggt
     6601 agctcccctg ggcccggtcg aacaccacgg gccagccacg gctgtaactg cgtacttcgg
     6661 attcgagttc ctcgaaaatg ctcatcacgt tttctttctc cctgagatcc ccggatttac
     6721 gttctattga ctgagcggac cgattcggta caggtcctcc tgctcatgcg ttccttcctc
     6781 ggccgggaaa tccgccgcgg agaacagatc ctggcgttcg acggtggcgt cccaccgctt
     6841 ggcgaacgag gcgaacaacc ggatcgaagc ctcgttgtcc ggcgtgatcg tggtttcgag
     6901 gtaccgcacg ccctgcgcgg tcagacggcc gaacagctcg tcgagcaggg ctccggccag
     6961 gccttttccg cgctgggacg cgtcgaccgc gacctgccac acgaagccgg tgtcctgctt
     7021 gcggtatccg atcacgaagc cgaccgggac gccgtcgact ttcgcgacga ccgaggtgtc
     7081 tgcgaaatcg tggcaccaca gcaaataggc gtaaggcgtg ttgagatcga gcttcttcga
     7141 atcgcgggcg attctccaga gcgcggcacc gtctgccttg gtcggagttt cgatcaagtg
     7201 ctttccggac atgtccaaga aacgtaccgg aggattcggg cgcggtcaga tcagcgcggc
     7261 ggcggcaacg cggctacctg caacaacctc gaaacacacg tgagaaaggt aaccgtaacg
     7321 tttgaagcgc agcatgggtg ggtaaggccg cttcggtgcg gtctcactgc acgtgacgat
     7381 accaaccgtt accacttcgt cgcacggtca ggacgcgatt cgggtcccga ttccgctatt
     7441 cacgacgtcg cttgcgaccc acgagcgaac cgatcggcgt ccagatggcg ggcaggacga
     7501 gcagcaggca gacgaccagc caccacaccg gcgcctcgcc gcgccgccag cccgcgacga
     7561 cgccaccgag ccagccgaag agcgggtcac gcaccggtgg caggaacgcg gcgaacagca
     7621 gggccgccac caccagcacg ccgagcaccg tgagcaccat ccgccacgtc ttgcgcaacg
     7681 tcatcaggct gaccaccagg aacacgacgg cgcccgcggc gaccggcacg ccgacccact
     7741 gcaggaccgg accgccctgt ccaccgagga cgaagcccgc cagcacggtg atcagcgcgg
     7801 cgagcacgtt ccacggcgac ggcagggtgg ccgcaccccg cgtgatccac cacgcgctgc
     7861 gcccggcggc cttggcgaag gtcgcggcgg gctggaccga cttcggcacc cgcgtcgccc
     7921 ccgcggccgc gttgaggccg gcggcacccg ccttgaccag cgtcttcgtc aggagcatcg
     7981 tcccgcgttc gtgctcgaac cgttcgccgg agacctggca cgcctggaac gcgcgctggg
     8041 tctgctcgtc ggtttccggc tcgttctcga tcatccggcg gaaccgttcc gacggtttcc
     8101 cgttcccctt ggccgttttc gcgtcgtgtc cacagtggag gccgacacac ggcagttcct
     8161 cgcgcgcgat ctccagctgc cgaaccctgg cgagcaccat cgccgtcacc ggcatgctga
     8221 tcggcagggc ggcttcccct tcgacatcgg cgagttcgcc gtcgagaccg aggaaagcca
     8281 gctccccggt caattgcgca cgcagggcgt ccgcttcctc ttgggacaga ccgtcttccg
     8341 tgcccggctg tcgccagccg atccccttga aggtgtcccg cacctgtgcc cggaacgcct
     8401 cgcgtccgac gacgtcccgc agcagccgca ggcgtttcgg gtcgagcagg cactgcacca
     8461 gccagcccgc gccgtcgacg cggccccaca tccagtcgtt cgcgcgccag gacgccttgt
     8521 agaacgcgcc gaagtagtcg gcctgcatcc cggtgagctt gtcccaggaa cgacgccgtt
     8581 tcatgtccat cagcgtccgc gaatccgcgc tcacctggac gagatcgacc cgctggtcga
     8641 ccgacggcgg ctgcgcgagc agtccccggg tcgcgatgtg gagacgtacc agccgcgagg
     8701 tgacgacagg atctccggcg cttttgtcgt ccgatgtgag cccggtccag gccagcaatg
     8761 tgctgaccgt gtcggcggcc tcggtgtcgg gtttcgcttg tgccagcggc aaaaggatcg
     8821 gcgtcgcctg ccggagcgcg gcgacgacga gcggccacgc ctccttcagc gtgtccgacc
     8881 ggccgagccc cgcccattcc ttggcgaggc cttcgatcca cgcggcgagc gtgacatcgg
     8941 gcgacggcgg accctgttcg gcgacccagc ccgagagccg tttcccgcgc gcggccttgt
     9001 ggcgcgcttc gtgcagcagc acccggcaac ggttgagctg caccgcctgc gccggcgacg
     9061 gatcgagccg gaagcccgcg ttcaccagtt gcagcccggt ggcgaccgaa tcgtcgagcg
     9121 cggtggtgcg gaaggcgacc aggtccgcga ggtcgcaggt gtccttcggc ggctcggtgg
     9181 gcaggccgtc gcgcagcccg gcggccgcgg ccgcacgcag ctgcgccgcc gtgcccgacg
     9241 cccactggcg gccttgatcc tccacatcgg acagtgaaag cctgcgggtc gcctcgcgga
     9301 ccagcgcggt ggcgtcctcc tgcacccggc ggtcgcggta gtcgttcatc agccgctggt
     9361 cgaccagcga gtccggcccg ccgcgcaccc ccatcgcggc cagggagacc ctggtccccc
     9421 tggtgcggac gacggcgtcg ttgtgcctgg tcaggtcttc cagttcggcg ctgatcgtct
     9481 ggctcagcac ggtgccgacg accttgctca tcgcgctgcc cagcagcggc gggcactcgg
     9541 gatcgaccgc gacccgctcg gcctcgcgtt cggccgtcgg caccacgtac agcagcagcc
     9601 gccggacgtc cgaatgcgac tgccgttcga agatctcccg cagcgccggc cgcagcggct
     9661 tgttgagcag gacaccgccg tcggtgagcc aatgcgaccg ggtgacgtcg gtgtacttcg
     9721 tcatgtccgg atgcagctgg ccggtcttct cgccgatcgg catcctggag agctcgaacg
     9781 caccggggaa cgacgcggtg gaccgcgccg cgagcgcgag cgggccgacg acgtcatccg
     9841 tccacaaagg agcgtcgaag cggaacagca ggcggtgttc ggtgtcccgc accaggtttc
     9901 cgagcgcgtc gtcgaagcgg gtcgtctcgc cgtcgatcat cgtgccggtg agcagcacgg
     9961 tgatgtcggg acagtccggt ttcaccgtcg ccttgtcggt gatccggtgc aacgcgtcct
    10021 tgagatcacc gagcagcacc ttgtcgccgt cgagcagcga acgcggctcc ttctcgccgg
    10081 ggtcgcggat caggttgtcg agcgatccgg tgctgatcca cgtgtcgcgc agcacctgcg
    10141 ggctcgaccg gaacgcctcg gccagcccga ggcacgccgc gttgatgccg cccgcgctag
    10201 tgccggtgag cacgtcgagc gagacggtgg cgttgagcag gtcgagcaag gcccggtacg
    10261 ggctcttcga ttccggtgtt ctcgacgcct gcaacagatg cgaggtctcg gtggcgaccc
    10321 cgcccatcca gaccgcgaga ctcgccccgc ccaccatggt catcgccagc cggatctcct
    10381 gcggccacgg
//
