LOCUS       NC_021252              10366 bp    DNA     circular CON 28-MAR-2024
DEFINITION  Amycolatopsis keratiniphila, complete sequence.
ACCESSION   NC_021252
VERSION     NC_021252.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis keratiniphila
  ORGANISM  Amycolatopsis keratiniphila
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis; Amycolatopsis japonica group.
REFERENCE   1  (bases 1 to 8948591)
  AUTHORS   Tang,B., Wang,Q., Yang,M., Xie,F., Zhu,Y., Zhuo,Y., Wang,S., Gao,H.,
            Ding,X., Zhang,L., Zhao,G. and Zheng,H.
  TITLE     ContigScape: a Cytoscape plugin facilitating microbial genome gap
            closing
  JOURNAL   BMC Genomics 14, 289 (2013)
   PUBMED   23627759
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8948591)
  AUTHORS   Xu,L., Zhao,W., Ge,M. and Zhao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2012) Shanghai Laiyi Center for Biopharmaceuticals
            R&D, Shanghai 201203, China
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_000400635.2
            Current Name                      :: Amycolatopsis keratiniphila
            Previous Name :: Amycolatopsis orientalis HCCB10007
            Date Updated                      :: 2018-10-26
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_001620365.2
            A1 Name                           :: Amycolatopsis keratiniphila
            A1 ANI                            :: 96.94781%
            A1 Query Coverage                 :: 84.86%
            A1 Subject Coverage               :: 83.93%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_000478275.1
            A2 Name :: Amycolatopsis orientalis HCCB10007
            A2 ANI                            :: 90.22668%
            A2 Query Coverage                 :: 73.73%
            A2 Subject Coverage               :: 73.08%
            ##Taxonomic-Update-Statistics-END##
            ##Assembly-Data-START##
            Assembly Method                   :: Newbler v. v2.5
            Sequencing Technology :: Sanger dideoxy sequencing; 454; Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000400635.2-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 02:04:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,307
            CDSs (total)                      :: 8,242
            Genes (coding)                    :: 8,193
            CDSs (with protein)               :: 8,193
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 14 of 49
            Pseudo Genes (incomplete)         :: 33 of 49
            Pseudo Genes (internal stop)      :: 3 of 49
            Pseudo Genes (multiple problems)  :: 1 of 49
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:56:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3938984
            Orig. end                         :: 3949350
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003410.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from Shanghai Laiyi Center for
            Biopharmaceuticals R&D, Shanghai 201203, P.R. China,
            hccbred@gmail.com.
            The organism was changed from 'Amycolatopsis orientalis HCCB10007'
            to 'Amycolatopsis keratiniphila' in October 2018.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10366
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="14"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10366
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10366
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5366](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5366
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(450..462)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     gene            complement(909..1301)
                     /locus_tag="AORI_RS58925"
                     /old_locus_tag="AORI_3591"
     CDS             complement(909..1301)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636383.1"
                     /locus_tag="AORI_RS58925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_3591"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_016333942.1"
                     /transl_table=11
                     /translation="MSNLDIIAAHYAASDRGDLAGMLAPLGPDTTWTEAAGFPYAGTYT
                     GPEEVRDNVFGAIAKDWDGYTFTLGDLLDAGDTVIGLGTYQAKHRETGRSFTARVAHVW
                     RFDGGKVTSFEQIVDSVPVVEAMENR"
     gene            complement(1311..2078)
                     /locus_tag="AORI_RS58930"
                     /old_locus_tag="AORI_3592"
     CDS             complement(1311..2078)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333943.1"
                     /locus_tag="AORI_RS58930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_3592"
                     /product="MoaF C-terminal domain-containing protein"
                     /protein_id="WP_016333943.1"
                     /transl_table=11
                     /translation="MPEAPEEQWRTYDEFAAGIATYRLPNADLSGRELTVTLDDGKTLA
                     LRFEDAETVTCNGVKDPYDAVAVRDDVFFVNLPHTSVEGEALTVVFSTTTHRALAVRSV
                     IGAEDVEGVPRVSQTFWSGTTDAGTPSGEVPGPSRDLIGKRNVYRYSPEHLYEHVYVSS
                     QRYAWQCLEGVQRGHGDMDLSTVWKFQDGLYLFCFREFRIAVASVWLHDLGYALKTTGV
                     FLGLTGDGRSEHSRAGGHIYPLGAVAYPDAQPV"
     gene            complement(2119..3099)
                     /locus_tag="AORI_RS58935"
                     /old_locus_tag="AORI_3593"
     CDS             complement(2119..3099)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1015: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333944.1"
                     /locus_tag="AORI_RS58935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_3593"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_016333944.1"
                     /transl_table=11
                     /translation="MTTATADPTTTTVARDFTDFRTVVSQSFVPLHVTSEHRDHFRGRI
                     RSCGADDVQLTEVTASAHVVERTPELIARADRHYYKLSLILSGTGLLVQDNREAMLRPG
                     DVALYDTHRPYTLAFEEDFRTLVVMFPQRLIDLPRDLVGQLTAVPMSGKRGMGNVVVPF
                     LAQLGSNLDQLGGPAGVRLAHTAVDLLATLFASELDLARASAGPHHELMRRVLAYIDAN
                     LSSTDLGPAQIAAEHYISTRHLHGLFHEQGTTVSGWIRTRRLEHCRRELLDPVHTSRPV
                     AAIAAKWGFVDAPHFSRVFKTEFGCSPSELRRGLTVSDRLLPPSA"
     gene            complement(3181..3663)
                     /locus_tag="AORI_RS58940"
                     /old_locus_tag="AORI_3594"
     CDS             complement(3181..3663)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333945.1"
                     /locus_tag="AORI_RS58940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_3594"
                     /product="hypothetical protein"
                     /protein_id="WP_016333945.1"
                     /transl_table=11
                     /translation="MKTDREGTEAPELPAFTVIGCLLGFFMTVLVLIGPALLWISTRFD
                     PGDDDRLVVVAMGAVFTLGGAGTVGSHLWQNAKARELHRGLDRKGVRANAEVVSSKRIW
                     VGEVSTPADDVDLVVTGPGVAPFSARVLTEDVGRYETGTVVPVDVDPGTLLFRLAD"
     misc_feature    3671..3688
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.61"
                     /tool="antismash"
     gene            complement(3754..4665)
                     /locus_tag="AORI_RS58945"
                     /old_locus_tag="AORI_3595"
     CDS             complement(3754..4665)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039924.3"
                     /locus_tag="AORI_RS58945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_3595"
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="WP_158509131.1"
                     /transl_table=11
                     /translation="MSVYLLRKPRTEVVLGGWRSQAKRRLTQSTRPLLDLVPASGSSPD
                     FLTPPGGGTLCEGIDTLLSTPRSRLAADFAHLAEERPLPSWTVDLAEGRPPALRMVANA
                     LREYFHTCLAPHWDAVATTVHHDHTRRAQLMADQGIDAVLSTLSPAATWRFPVLSIDYL
                     FDHDVRLDGRGLLLQPVVFGRGPNPTTLIDPALPPVLVYSVPLAPDVITPVEPDRRRAG
                     DPIVALLGTTRTRILRALDSTGATTTELARRAATPLPTASRHATALRDANLITSRRHKN
                     MVIHAITPLGTALLHGRQPRLN"
     misc_feature    complement(3784..3786)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(4651..4653)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(4994..5009)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.01"
                     /tool="antismash"
     gene            5001..5366
                     /locus_tag="AORI_RS83380"
                     /old_locus_tag="AORI_3596"
     CDS             5001..5366
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /locus_tag="AORI_RS83380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_3596"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_228688373.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.5e-11,
                     bitscore: 43.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRILAGALLGSGLMVAGLFGAAPVASAAPTESVSVSVALSDSVPF
                     ACQNISGGTWCYGTESAGSGQKKCYSHYLHPTKRHSATAILGSRQNKKTANRGQWAKAE
                     VIGSSRDTCNAYYNSDA"
     misc_feature    5022..5024
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5421..6737)
                     /locus_tag="AORI_RS58955"
                     /old_locus_tag="AORI_3597"
     CDS             complement(5421..6737)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636379.1"
                     /locus_tag="AORI_RS58955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_3597"
                     /product="cytochrome P450"
                     /protein_id="WP_016333948.1"
                     /sec_met_domain="p450 (E-value: 8.4e-20, bitscore: 69.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFALTFRHKAEAIAKPVTRWSIGHAIPRVALRAAARRGDLQGRLS
                     VEASGGADLTGLFDEIRSHGAIATTRVGHVTTRHAACKEILTSDAFKTVRFVPDNPLLN
                     RLVAWSSSGLIHPIEPPSLLASEPPDHTRYRKLVTRVFTARAVEQLRERTQAITDELLD
                     GLDPTSPVDLIEQYCGLLPVTVISQILGVPPEERDKVLALGGAAAPSLDLGLPWRTFRT
                     VEATLAEFDSWLTVHLEKLRANPGDNLLSKLIAAREDGVGLTERELKATAGLVLAAGFE
                     TTVNLLGNGIALLTRHPDQLAKLRADPGLWSNAVDEVLRYDPPVLLTGRLAARDTEIGG
                     TPIARGAIVSTVLAGANRDPEIFDDPAAFDVGRANARDHVSFGAGRHYCLGASLARMEG
                     EIGLRSIFERFPDLRLLSGGRRRETRILRGFETLPAALI"
     gene            6867..8144
                     /locus_tag="AORI_RS58960"
                     /old_locus_tag="AORI_3598"
     CDS             6867..8144
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1273: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029099.1"
                     /locus_tag="AORI_RS58960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_3598"
                     /product="hemolysin family protein"
                     /protein_id="WP_016333949.1"
                     /transl_table=11
                     /translation="MGGYGFDIALVAVLVVLNAVFAGSEMALISLREGQLRALERDGRA
                     AARTLVRLARDPNRFLATIQIGITLAGFLASATAAVSLAQPLVPLLGFLGNAAGAVAVA
                     LVTMVLTFLTLVLGELAPKRLAMQNALRWALLVARPLNLLSAISRPVVWALSVSTNLVV
                     RALGGRPEADPDQMSPEELRELVSAQRGLNAEQRMIINGALEIHERRLREVLVPRRAVL
                     TLAAEQDLLSARRQLAESGHSRAPVARGGHLDDVVGVVRLRDLLDDHADLAEVVRPAVV
                     FPDSLRVSDALRSFKAEREQMALVVDEHGAVAGMVTLEDLLEEIVGEIYDETDRDVLAV
                     RDGKDGSLVLPGTFPVHDLVDVGVELRDAPPGEYATIAGLILVILGRIPEKPGDRVVVS
                     GWTAEVLGVEHHAITRVALHRGTNPE"
     gene            8211..9890
                     /gene="ilvD"
                     /locus_tag="AORI_RS58965"
                     /old_locus_tag="AORI_3599"
     CDS             8211..9890
                     /EC_number="4.2.1.9"
                     /GO_function="GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ilvD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005156871.1"
                     /locus_tag="AORI_RS58965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_3599"
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="WP_016333950.1"
                     /transl_table=11
                     /translation="MPQDQPDLKPRSRDVTDGLERAAARGMLRAVGMGDDDFAKPQIGV
                     ASSWNEITPCNLSLDRLAKAVKNGVHAAGGYPLEFGTISVSDGISMGHEGMHFSLVSRE
                     VIADSVETVMMAERLDGSVLLAGCDKSLPGMLMAAARLDLASVFLYAGSIMPGQVDGQD
                     VTIIDAFEAVGACLAGKISRAEVDKIERAICPGEGACGGMYTANTMASVAEALGMSLPG
                     SAAPPAVDRRRDGFAHRSGEAVVGMLRQGITARQIMTMEAFENAIAVVMALGGSTNAVL
                     HLLAIAREAEVDLRIDDFNRIGDKVPHLGDLKPFGKYVMNDVDKVGGIPVVMKALLDAG
                     LMHGDVLTVTGKTMAENLEGIAPAGVDGEIIRPLDRPIHKTGGLTILKGSLAPEGAVVK
                     SAGFDESTFTGTARVFDGERAALDALAEGRIVAGDVVVIRYEGPKGGPGMREMLAITGA
                     IKGAGLGKDVLLITDGRFSGGTTGLCVGHIAPEAVDAGPIAFVRDGDPITLDVANRTLE
                     VGIDDIEARKEGWTPKPPAYTRGVLGKYAKVVRSAAHGAVCE"
     misc_feature    9901..9912
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    complement(10218..10231)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.5"
                     /tool="antismash"
     misc_feature    complement(10284..10295)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
ORIGIN
        1 cagcgggatg ttgttcttgg tcatcagctc gcgccagtcc ttgttctgcg ggctggtcgc
       61 gtcggtgaac gggtagaact cggcgggcac gtacaccccg gcgccggcgt tggtgatggc
      121 cttggcgtag ttctcgctgt agacgctggt gagcagcagc cacgtgacgt cgttccagcc
      181 ctgcgccgcg gcggccttca gctgcccgat ggaatccggc tcgaccgggt tcaccgtgat
      241 cgccttgcag cccgcgttgc gcgccttgac gatgtaggag gtgtagtcgg aaccgccgta
      301 gggcacggtc gcgtcgaggt acttgagctt cttgccggtg atggccgacc actcgtcgat
      361 cgcggcctgg tacgacggca gcgtgttgcc cgcgatctcc agcagcgcgc agatgtcgtt
      421 cagcttcagc gtttccgagc cgtagagcag tgtcagcgtc atgtcgtggt acgggccgac
      481 gttgaccggg gcgatgttcg ggctggagaa acaggccggg tccacgccga tgccctgcat
      541 ggacaggatc ttctgctgct cgtagtactt gttgttgatc tcgcactcga tgaggctgga
      601 cgagccgacg agcgccaccg cctcgtcccg cccgacgacc tcgcgggccg cggcggccgc
      661 ggccgcgggg tcgcccttgt cgtcgagggc cttgtactgg atctgcctgc cgttcacgcc
      721 gccgctcgcg ttggccgcgt cgaagaccgc cttggccgcc tgtgaggcct cggggaaggt
      781 cgccgcgccg ctcagcgcgt tgaccgaacc gaccacgatc ggcccgctcg caccgctgtc
      841 ggatccggcg cacccggccg tcgcgagcag gacggccgag gcgagggcga cgaggcgccg
      901 cgcgggtttc accggttctc catggcttcg accacgggca ccgagtcgac gatctgctcg
      961 aacgaggtca ccttgccgcc gtcgaaccgc cacacatgcg cgacgcgggc ggtgaacgaa
     1021 cggccggtct cgcggtgctt cgcctggtag gtgccgagac cgatcaccgt gtcgccggcg
     1081 tcgagaaggt cgccgagggt gaaggtgtag ccgtcccagt ccttcgcgat ggcgccgaag
     1141 acgttgtccc gcacctcttc cggaccggtg tacgtgccgg cgtaagggaa accggccgcc
     1201 tccgtccagg tggtgtcggg accgagcggg gccagcatgc ccgccagatc gccccggtcg
     1261 ctcgcggcgt agtgcgcggc gatgatgtcg agattggaca ctgaggctcc ttagaccggt
     1321 tgggcgtcgg ggtaggcgac ggcaccgagt gggtagatgt gcccgcccgc gcgggagtgc
     1381 tccgaccggc cgtcgccggt caggccgagg aagacgccgg tggtcttcag cgcgtagccg
     1441 aggtcgtgca gccacacact ggccacggcg atgcggaatt cgcggaagca gaacaggtag
     1501 agcccgtcct ggaacttcca gaccgtcgac aggtccatgt ctccgtggcc gcgctgcacg
     1561 ccttcgaggc attgccaggc atagcgttgc gaagacacgt acacgtgctc gtagaggtgt
     1621 tccgggctgt agcggtagac gttgcgtttg ccgatcaggt cgcgcgacgg gccgggaacc
     1681 tcgcctgacg gcgttcccgc gtcggtcgtg ccggaccaga acgtctggga gacacgggga
     1741 acgccttcga catcctcggc gccgatgacc gagcgcacgg cgagcgcgcg atgcgtcgtc
     1801 gtggagaaga cgacggtgag cgcttcccct tcgacgctgg tgtgtgggag attgacgaag
     1861 aagacgtcat cccggacggc gaccgcgtcg tacggatcct tgacgccgtt gcaggtgacg
     1921 gtttcagcgt cttcgaaccg cagcgcgagc gtcttcccgt cgtcgagggt gacggtgagc
     1981 tcgcgtcccg acaggtccgc gttcggcagg cggtaggtgg ctattccggc ggcgaactcg
     2041 tcataggtac gccactgttc ctcgggtgct tcgggcatgc ggtcatcgtc gaggcgaccg
     2101 ccctggcagg ggaagccgtc acgcgctggg cggcaacaac cgatcgctca cggtcaagcc
     2161 gcgtcgcagt tcgctcggcg agcagccgaa ttccgtcttg aacacgcggc tgaaatgcgg
     2221 cgcgtcgacg aaaccccact tcgccgcgat ggccgcgacg ggccgcgaag tgtgcacggg
     2281 gtcgagcagt tcgcggcggc agtgttcgag gcgccgggtg cggatccagc ccgagacggt
     2341 ggtgccctgc tcgtggaaga ggccgtgcag atgccgggtg gagatgtagt gctcggcggc
     2401 gatctgtgcc ggtcccaggt cggtggagga gaggttggcg tcgatgtagg cgaggacgcg
     2461 gcgcatcagt tcgtgatgcg gcccggcgct cgcgcgggcg aggtcgagct cgctggcgaa
     2521 cagggtggcg agcagatcga ccgcggtatg cgcgagccgg acgcccgccg gcccgccgag
     2581 ctggtcgagg ttgctgccga gctgcgcgag gaagggcacc accacgttgc ccatgccccg
     2641 cttgcccgac atcggcaccg cggtcagctg gccgaccagg tcgcggggga gatcgatgag
     2701 ccgctgcggg aacatcacca cgagcgtgcg gaaatcctct tcgaacgcca gggtgtacgg
     2761 ccggtgcgtg tcgtacaacg ccacgtcgcc gggacgcagc atcgcctcac ggttgtcctg
     2821 caccagcaga ccggtgccgg acaggatcag gctcagcttg tagtagtgcc tgtccgccct
     2881 ggcgatgagt tcgggggtcc gctcgaccac atgcgccgac gcggtcacct cggtcagctg
     2941 cacgtcgtcg gctccgcacg agcggatgcg gccgcggaag tggtcgcggt gctcgctcgt
     3001 cacgtgcagt ggcacgaagg attgtgacac caccgtgcgg aaatcggtga aatctcgcgc
     3061 gaccgtggtg gtggtcgggt cggcggtggc ggtggtcacg ggggtcacct cgcagcatcg
     3121 gggggtgaat ttcatatacg ataagcgata cgatgctgcc gggcaatgat cgcggcgagg
     3181 tcagtcggcg aggcggaaca gaagggttcc cgggtccacg tcgacgggta ccaccgtgcc
     3241 cgtttcgtag cggccgacgt cttcggtgag cacgcgcgca ctgaacggag cgactcccgg
     3301 gccggtgacg acgagatcga cgtcgtcggc gggcgtgctc acctcgccga cccagatccg
     3361 cttgctggac accacttcgg cgttcgcgcg gacccctttc cggtcgagcc cgcgatggag
     3421 ctccctggcc ttggcgttct gccagaggtg cgagccgacg gtgccggcgc cgccgagggt
     3481 gaacacggcg cccatggcga cgacgaccag gcggtcgtcg tcgccgggat cgaagcgggt
     3541 gctgatccag agcagggccg ggccgatcag gaccagtacc gtcatgaaga agcccaagag
     3601 gcagccgatg acggtgaagg cgggcagttc cggggcttcg gtgccttcgc ggtccgtctt
     3661 caccggcgtg aggttactgt cctgttccgc gtgccggttc ggtaaaaatg cccgccatct
     3721 cgtgatgagg ggaaaggccg gatccggcgc tcgtcagttc aggcgcggtt gccgtccgtg
     3781 gagtaacgcg gtgccgagcg gggtgatcgc gtggatcacc atgttcttgt ggcggcgact
     3841 ggtgatcagg ttggcgtcgc ggagcgcggt ggcgtggcgg ctggcggtgg gcagcggtgt
     3901 ggcggcccgg cgggcgagtt cggtggtggt ggcgccggtg ctgtcgagag cgcgcaggat
     3961 acgggtgcgg gtggtaccga gcagggcgac gatcggatcg ccggcgcgtc ggcgatccgg
     4021 ttccaccggg gtgatgacgt cgggggcgag cggcaccgaa tagaccagga cgggcggaag
     4081 tgccgggtcg atgagcgtgg tggggttggg gccgcggccg aagaccaccg gctgcagcag
     4141 caggcctctt ccgtcgagtc gaacgtcgtg gtcgaagaga taatcgatgc tcagcacggg
     4201 aaagcgccat gtcgccgcgg ggctcagggt gctcaggacg gcgtcgatgc cttgatcggc
     4261 catgagttgg gcgcggcggg tgtgatcgtg gtgcacggtg gtggcgaccg cgtcccagtg
     4321 cggggcgagg caggtgtgga agtactcccg cagtgcgttg gcgaccatgc gcaacgcggg
     4381 tggccgccct tcggcgagat cgacggtcca ggaagggagt ggccgttcct ccgccaggtg
     4441 cgcgaagtcg gccgccagcc gggaccgtgg cgtgctcagc agggtgtcga tgccctcgca
     4501 caaggtgccc ccgccaggcg gggtcaggaa gtcgggggag gagcctgacg cgggaaccag
     4561 atccagcaac ggccgggtgg actgcgtcag ccgccgcttg gcctggcttc tccagccacc
     4621 cagcacgacc tcggtccggg gtttacgcag taaatacaca ctcatgagga cctcccacaa
     4681 cggatccggg cgtcgcgcta tccggatacg ggccaggtcc gcgagtgtga aatggattcg
     4741 caacattccc tcttcctccc cccgtctacg acgctactgc ggaaaacatg atcgttgcca
     4801 ccgtggccgc gtgaagcctg ccggtgatcg atcatgacgc gccggaatcg gccgctgatc
     4861 agcgtgtttg cggcaggatg aagatgcttt cccctcttcg gtgggccgtc atagtattgg
     4921 cgtcgaattc gcgagtgggg gtaaatcgcc ttactggctg tcttcttccc atgtattcaa
     4981 ttcgattgcg agggatgatc atgagaattc tggcgggagc attactggga tcgggtttga
     5041 tggtggcggg tctgttcggg gcggcgccgg tggcctcggc ggctccgacg gagagcgtga
     5101 gcgtgtccgt ggcgctgagc gactcggtgc cgttcgcttg ccagaacatc agcggcggta
     5161 cgtggtgtta cgggaccgaa tcggcgggca gcgggcagaa gaagtgttac tcgcactatc
     5221 tgcaccccac caagcgccac agcgcgaccg ccattctcgg ctccaggcag aacaagaaaa
     5281 ccgcgaatcg cgggcaatgg gccaaggccg aggtgatcgg ttcgagtaga gacacctgca
     5341 acgcgtacta caactccgac gcgtgatccg accttcgcgg ggtgccggac caccgggttc
     5401 gccaccccgc gaaggcgggt tcagatgagc gcggccggaa gcgtttcgaa gccgcgcagg
     5461 atccgggtct cccgccgtcg gccgccactc agcagccgca gatcgggaaa gcgctcgaag
     5521 atcgaccgca gcccgatctc gccttccatc ctcgccagcg aagcaccgag acagtagtgc
     5581 cgcccggcgc cgaacgacac gtggtcccgc gcgttcgccc ggccgacatc gaaggcggcc
     5641 gggtcgtcga agatctccgg gtcccggttg gcgcccgcga gcacggtgct gacgatcgcc
     5701 ccgcgggcga tgggggtccc gccgatctcg gtgtcccggg cggccagcct gccggtgagc
     5761 aaaacgggcg ggtcgtaacg gaggacctcg tcgacggcgt tgctccacag gcccggatcc
     5821 gcccgcagtt ttgcgagttg gtccggatgc cgcgtcagca gggcgattcc gttgccgaga
     5881 aggttgaccg tggtttcgaa gccggccgcc agcaccagcc ccgcggtggc cttgagctcc
     5941 cgctcggtca ggcccacgcc gtcctcgcgc gcggcgatca gcttgctgag caggttgtcg
     6001 ccggggttcg cccgcagctt ctccagatgc acggtgagcc acgagtcgaa ctcggccagc
     6061 gtcgcctcca ccgtccggaa cgttcgccac ggcaggccga ggtccaggct cggcgccgcg
     6121 gcaccgccca gtgcgagcac cttgtcgcgt tcctccggcg gcacgccgag gatctggctg
     6181 atcacggtga ccggcaggag cccgcagtac tgctcgatga gatcgaccgg gctcgtcggg
     6241 tcgagaccgt cgagcagttc gtcggtgatc gcctgcgtgc gttcccgcag ctgctccacc
     6301 gcgcgggcgg tgaagacgcg ggtgaccagc ttccggtagc gcgtgtgatc gggcggttcg
     6361 ctggcgagca gcgacggcgg ctcgatcgga tggatcagac cggacgacga ccaggcgacg
     6421 agccggttga gcagcgggtt gtccgggacg aaccgcacgg tcttgaacgc gtcactggtc
     6481 aggatctcct tgcacgccgc atgccgggtg gtgacgtgcc cgacccgggt ggtggcgatg
     6541 gcgccgtgcg agcggatctc gtcgaacagg ccggtcaggt ccgctccacc actcgcctcg
     6601 acggacaacc tgccctggag gtcgccccgg cgcgccgcag cccgcagggc gacccggggg
     6661 atggcatggc cgatgctcca gcgggtgacc ggtttggcga tcgcctccgc cttgtgccgg
     6721 aaggtcagtg cgaacatcag gactccgtcc gtgcggcttc cttcgaaggt agcggaacga
     6781 cgtgtcagta gcgagtggct ccgatatctg cgatcccgct tcgcgctcgg taatgttctg
     6841 cacttcgggc gggccgggga gtgcttgtgg gcggctacgg gttcgacatc gcgctggtcg
     6901 cggtgctggt ggtgctcaac gcggtgttcg cgggcagcga gatggcgctg atctcgttgc
     6961 gcgaagggca gctgcgggcg ttggaacggg acggccgcgc cgccgcccgc accctggtgc
     7021 ggctggcgcg cgacccgaat cggttcctgg cgacgatcca gatcggcatc acgctcgccg
     7081 ggttcctcgc ctcggcgacg gcggcggtgt ccctcgcgca acccctggtg ccgctgctgg
     7141 gtttcctcgg caacgcggcc ggggcggtgg ccgtcgcgct ggtgacgatg gtcctcacgt
     7201 tcctgaccct ggtgctcggc gaactggcgc cgaagcggct ggcgatgcag aacgcgctgc
     7261 ggtgggcgtt gctggtggcg cgcccgctga acctcttgtc ggcgatctcg cggcccgtgg
     7321 tgtgggcgct gagcgtgtcg acgaacctcg tcgtacgggc gctgggcggg cggccggaag
     7381 ccgatccgga ccagatgtca cccgaggaac tccgggaact ggtttcggcg cagcgcggcc
     7441 tgaacgccga gcagcgcatg atcatcaacg gtgccttgga gatccacgaa cgacggctgc
     7501 gggaggtgct cgtgccccgc cgcgcggtgc tgaccctggc cgccgaacag gatctgctct
     7561 cggcacgcag gcagctggcc gaatccggcc actcccgggc gccggtcgcg cgcggcggcc
     7621 atctggacga cgtcgtcggt gtcgtgcggt tgcgtgacct gctcgacgac cacgcagacc
     7681 tggccgaagt cgtccggccg gcggtggtgt tccccgattc gctgcgggtg tcggacgcgc
     7741 tccggagttt caaggccgaa cgggagcaga tggcgctggt ggtcgacgag cacggcgccg
     7801 tcgcgggcat ggtcacgctg gaggatctgc tggaggagat cgtcggcgag atctacgacg
     7861 agaccgaccg tgacgtgctg gccgtacgcg acggcaagga cggttcgctg gtcctgcccg
     7921 gcaccttccc ggtgcacgac ctcgtcgacg tcggggtcga actgcgcgac gcgccgccgg
     7981 gggagtacgc gaccatcgcg gggctgatcc tcgtgatcct cgggcggatc ccggagaagc
     8041 cgggcgaccg cgtggtggtc tccggctgga cggccgaggt gctcggcgtc gagcaccatg
     8101 cgatcacgcg ggtggcgctg caccgtggga cgaacccgga gtgatgagtt gcgccggacg
     8161 cgcggggttg gcagcgtgcc cggtgtcccg ccacgaagga aggtgccccg atgccgcaag
     8221 accagccgga tttgaaacct cgttcgcgcg acgtgaccga cgggctggaa cgcgcggcgg
     8281 cccgcgggat gctccgcgcg gtcgggatgg gcgacgacga cttcgccaaa ccgcagatcg
     8341 gtgtggcgtc ctcgtggaac gagatcaccc cgtgcaatct gtcgctcgac cggctggcca
     8401 aggcggtcaa gaacggcgtg cacgcggccg gcggctatcc gctggagttc ggcacgatct
     8461 cggtgtccga cggtatttcc atgggacacg agggaatgca cttctccctg gtgtcccgcg
     8521 aggtgatcgc cgactccgtc gagacggtga tgatggccga gcggctggac ggctccgtgc
     8581 tgctggccgg ctgcgacaag tccctgccgg gcatgctcat ggccgcggcc aggctcgatc
     8641 tcgcctccgt cttcctttac gccggctcga tcatgcccgg tcaggtcgac ggccaggacg
     8701 tcaccatcat cgacgcgttc gaagcggtgg gcgcctgtct ggccggcaag atcagccgcg
     8761 ccgaagtgga caagatcgaa cgcgcgatct gccccggcga aggggcctgc ggcggcatgt
     8821 acaccgccaa cacgatggcg tcggtcgccg aagcgctcgg catgtcgctg cccggatcgg
     8881 ccgccccgcc cgccgtcgac cggcgtcgtg acgggttcgc gcaccggtcg ggggaggccg
     8941 tggtcggcat gctccgccag ggcatcaccg cgcggcagat catgaccatg gaggcgttcg
     9001 agaacgccat cgccgtcgtg atggcgctgg gcggttcgac caacgccgtc ctccacctgc
     9061 tcgcaatcgc gcgcgaggcg gaggtcgatc tgcgcatcga cgacttcaac cggatcggcg
     9121 acaaggtccc gcacctcggc gatctcaagc cgttcgggaa gtacgtgatg aacgacgtcg
     9181 acaaggtcgg cggcatcccc gtggtgatga aggcgctgct cgacgccggg ctcatgcacg
     9241 gcgacgtgct caccgtgacc gggaagacga tggccgagaa cctcgaaggc atcgccccgg
     9301 ccggggtcga cggcgagatc atccgcccgc tcgaccggcc gatccacaag accggcgggc
     9361 tgaccatcct caagggctcg ctggcgcccg aaggcgcggt cgtgaagtcg gcgggcttcg
     9421 acgaatcgac cttcaccggt accgcacggg tcttcgacgg cgagcgggcc gctctcgacg
     9481 cgctcgccga gggccggatc gtcgccggcg acgtcgtcgt catccgttac gaaggcccca
     9541 agggcgggcc ggggatgcgg gagatgctcg ccatcaccgg cgcgatcaag ggcgccggac
     9601 tcggcaagga cgtcctgctc atcaccgacg gccggttcag cggcggcacg accggcctct
     9661 gcgtcggcca catcgccccg gaagccgtcg acgccgggcc gatcgccttc gtgcgcgacg
     9721 gtgacccgat caccctcgac gtcgcgaacc ggacgctgga agtcgggatc gacgacatcg
     9781 aggcacgcaa ggagggctgg acgcccaagc cgcccgcgta cacgcgcggg gtgctcggga
     9841 agtacgccaa ggtcgtgcga agcgccgcgc atggagccgt gtgcgagtag ggcggctgaa
     9901 gtacatgaag gcccccttcc tgtacccagg cgccagggtc ccggctaagc ggcctattcg
     9961 ctggtcaagt gggcaacgga ggcactcgtc acgctggcct gactccgtct cgactgtcca
    10021 caaggggacc cttccggggt gatcgtgccc gatttaggcg ttccgcaaag gggtcgtgag
    10081 tggcaaagag ggttagaacg acctttacca ctcacgaccc ctcggcataa tcacccattt
    10141 cgccatgaat aggacattcg gtcgccgaga agtgaccagt ggacggttgg agtctggtcg
    10201 actcccgtcg accagggcaa tcaagcgctt gacctgcgct tatgcgactt tgccgggacc
    10261 ctgccttcag agtagggaag ggggccttca tgtacttgga ggcccccacg aacgggtgat
    10321 gcgtaggccg gacagccccg agtacgtaat tcacccgatg tgcccc
//
