LOCUS       NC_021252              10816 bp    DNA     circular CON 28-MAR-2024
DEFINITION  Amycolatopsis keratiniphila, complete sequence.
ACCESSION   NC_021252
VERSION     NC_021252.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis keratiniphila
  ORGANISM  Amycolatopsis keratiniphila
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis; Amycolatopsis japonica group.
REFERENCE   1  (bases 1 to 8948591)
  AUTHORS   Tang,B., Wang,Q., Yang,M., Xie,F., Zhu,Y., Zhuo,Y., Wang,S., Gao,H.,
            Ding,X., Zhang,L., Zhao,G. and Zheng,H.
  TITLE     ContigScape: a Cytoscape plugin facilitating microbial genome gap
            closing
  JOURNAL   BMC Genomics 14, 289 (2013)
   PUBMED   23627759
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8948591)
  AUTHORS   Xu,L., Zhao,W., Ge,M. and Zhao,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2012) Shanghai Laiyi Center for Biopharmaceuticals
            R&D, Shanghai 201203, China
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_000400635.2
            Current Name                      :: Amycolatopsis keratiniphila
            Previous Name :: Amycolatopsis orientalis HCCB10007
            Date Updated                      :: 2018-10-26
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_001620365.2
            A1 Name                           :: Amycolatopsis keratiniphila
            A1 ANI                            :: 96.94781%
            A1 Query Coverage                 :: 84.86%
            A1 Subject Coverage               :: 83.93%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_000478275.1
            A2 Name :: Amycolatopsis orientalis HCCB10007
            A2 ANI                            :: 90.22668%
            A2 Query Coverage                 :: 73.73%
            A2 Subject Coverage               :: 73.08%
            ##Taxonomic-Update-Statistics-END##
            ##Assembly-Data-START##
            Assembly Method                   :: Newbler v. v2.5
            Sequencing Technology :: Sanger dideoxy sequencing; 454; Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000400635.2-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 02:04:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,307
            CDSs (total)                      :: 8,242
            Genes (coding)                    :: 8,193
            CDSs (with protein)               :: 8,193
            Genes (RNA)                       :: 65
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 14 of 49
            Pseudo Genes (incomplete)         :: 33 of 49
            Pseudo Genes (internal stop)      :: 3 of 49
            Pseudo Genes (multiple problems)  :: 1 of 49
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:56:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2806358
            Orig. end                         :: 2817174
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003410.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from Shanghai Laiyi Center for
            Biopharmaceuticals R&D, Shanghai 201203, P.R. China,
            hccbred@gmail.com.
            The organism was changed from 'Amycolatopsis orientalis HCCB10007'
            to 'Amycolatopsis keratiniphila' in October 2018.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10816
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10816
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10816
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5816](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5816)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            118..1191
                     /gene="hemE"
                     /locus_tag="AORI_RS54140"
                     /old_locus_tag="AORI_2630"
     CDS             118..1191
                     /EC_number="4.1.1.37"
                     /GO_function="GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005156993.1"
                     /locus_tag="AORI_RS54140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_2630"
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="WP_043848015.1"
                     /transl_table=11
                     /translation="MSPSVASPAQTVPAARRALPGAPFLAAARGERPAHTPVWFMRQAG
                     RSLPEYRALREGTSMFEACFDPEMLAEITLQPIRRHGVDAAILFSDIVVPLKAAGLDID
                     IVAGTGPVVAEPIRDAAGVRALPVLEPEQVERVAEGVRLLVGSLGETPLIGFAGAPFTL
                     ASYLIEGGPSRNHEHTKALMHSEPGLWHELAGRLADMAITFLSAQLDAGADAIQLFDSW
                     AGALSERDYREFVLPHSAKVLSAVARYGVPRIHFGVGTGELLVAMRDAGADVVGVDWRI
                     PLDEAVRRLGGGVVQGNLDPALLHGSWPVIEAEVRRILAEGRAADGHIFNLGHGVLPGV
                     DPDVLTRVVGLVHELQP"
     gene            1188..2567
                     /gene="hemG"
                     /locus_tag="AORI_RS54145"
                     /old_locus_tag="AORI_2631"
     CDS             1188..2567
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332996.1"
                     /locus_tag="AORI_RS54145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_2631"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_016332996.1"
                     /transl_table=11
                     /translation="MKTVAVVGGGISGLTAAYRLRTLLGEDARIVVFEATGSLGGKLRT
                     VELAGERYDVGAEAFLARRPEALALVREVGLGESLVHPTKARAKIHAGGSVLGLPPGTV
                     MGVPASAGAVAGMLSEKGRALVEAEASLPPLELPAEDVPLGPLLRERFGDELVDRLVDP
                     LLGGVYAGGADGLGLRATMPGLASAIDRGATSLTEGAASRLPASPSTAPVFGTLLGGLG
                     TLIARLAELSRAEIRTGTTVTALDRTPTGWKVDDLDADAVLLAVPAPSARRLLDGVAGV
                     ASSVFAKVELASMAVVALALPPGTALPESSGVLIGAGERDAAGKPFAAKAFTFSSRKWA
                     QYGEGPVLVRGSVGRFGEPGALHADDDELVRVVRDDLARLTGVTAEPIDILVTRWGGGL
                     PQYGTGHLVRVERIERAVAAVPGLAVAGATLHGVGLPACIATADAAARRIAGITPPHEG
                     S"
     gene            2593..3285
                     /gene="hemQ"
                     /locus_tag="AORI_RS54150"
                     /old_locus_tag="AORI_2632"
     CDS             2593..3285
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /locus_tag="AORI_RS54150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_2632"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_016332997.1"
                     /transl_table=11
                     /translation="MARLNYNELNDTIRYTAWSVFRAEQGKLGEDRAKATAETTEYLDA
                     LEAKGVVVRGVYDLSGLRADADYMIWWHAEEIEQVQAAYSGFRHTPLGRASAPVWSQVA
                     LHRPAEFNKSHIPAFLAGEEARKFICVYPFVRSYEWYLLPDDERRKMLADHGKEARDYP
                     DVRANTVASFALGDYEWILAFEADELHRIVDLMRHLRGTEARLHVREEIPFYTGTKVAP
                     AELIALLP"
     misc_feature    2635..2650
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.76"
                     /tool="antismash"
     gene            3297..4643
                     /locus_tag="AORI_RS54155"
                     /old_locus_tag="AORI_2633"
     CDS             3297..4643
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1189: L-carnitine dehydratase/bile acid-inducible"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033530.1"
                     /locus_tag="AORI_RS54155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_2633"
                     /product="CoA transferase"
                     /protein_id="WP_043848713.1"
                     /transl_table=11
                     /translation="MDTRVWTTLTGDTLAEDAVEITGPESVLPGTFRVEEAATTSIAAA
                     TLAAGELLRLRGIEPGKVSVDTRHAAAAFHSEQYLRVDGEPVPFSAPLSGDYRANDGWV
                     RLHCNYPRHVSAVCWGLGVPATRAAFEKVVAAKSKYEVESAVVGAGGAAAVLRTRDEWL
                     AHKQGQASSALPVADIRPLGTSEPVRLFESGRPLGGVRILDLTHVIAGPVAGRVLAAHG
                     ATVMHIGSAQLPTVPPLAVDTGMGKLSAYVDLETEAGRSRLRKLIARADVVLQGFRPGS
                     LTAKGFSPEAIAELRPGIVVADLSAYGWEGPWARRRGFDSLVQMASGIAEEGGRTAGLD
                     GPGPLPVQALDHATGWLTAAAIMTAVRQAATEGGSRHVRTSLAGTGEWLNSLGRKEAGP
                     AGFDGGEWLEDVDGQLGRVTRVKMAGTLPGSNPQWTEATHVPGSDRPVW"
     gene            complement(4660..5004)
                     /locus_tag="AORI_RS54160"
     CDS             complement(4660..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033529.1"
                     /locus_tag="AORI_RS54160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_043848016.1"
                     /transl_table=11
                     /translation="MTSPRERLAGQQAELLKALLAGGDAPAGFDADRLRIEANVMRNKQ
                     CRLAAYLRPDLAEALGDRFAALFREYATAHPKTDTIRARAYADAFGTWLVERGEVPKPR
                     GRFASWLRRI"
     gene            complement(5001..5816)
                     /locus_tag="AORI_RS54165"
                     /old_locus_tag="AORI_2634"
     CDS             complement(5001..5816)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033528.1"
                     /locus_tag="AORI_RS54165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_2634"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_043848714.1"
                     /sec_met_domain="DUF692 (E-value: 1.6e-80, bitscore: 269.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAADGSGTLGIGIGWRHELDLSIARLPGVDWVEVVAENLHAAHLP
                     ETLLALRDKGMPVLPHAVSLSLGGAEPLDTARVEHLAEITRELGAPLASDHVCFVRAGG
                     LDSGHLMPLPRTREALDVLVDNVKLTQSIVEVPFALENIAAVLEWPDAEYTESEFLREL
                     TDRTGCLLIVDVANLYANARNLGTDPERFLDEIPWERLAYTHMAGGVERDGVYHDTHAH
                     PVVPEVLDLLRELRRRTEPPGVLLERDDDYPSDAELAAELAALRDAVSP"
     gene            complement(5806..6735)
                     /locus_tag="AORI_RS54170"
                     /old_locus_tag="AORI_2635"
     CDS             complement(5806..6735)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033527.1"
                     /locus_tag="AORI_RS54170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_2635"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_016332999.1"
                     /transl_table=11
                     /translation="MEDTWGIPGPTFLAIYGALIVFPAVAGFAWTIMAKLGGKAPRAEL
                     SGPQPTVYEFAYLAGGPDRVVDTAIATLVDRGTLRVSSTKQLQLTGPEPADPIEKAVAK
                     GARPGYNATTRGIRDRLRMSGPMQALAKDLEARGLVVVDQTPRIRRVVYFLYLAVLVLG
                     VVRLIAGIAGDRPVGFLIPMLIAAVFATLISRALKNKRPGPRPTSEGARVLHKARSAHG
                     RERKRGIPNGPGYVGGAVLGGAAAAVAFGGLASYPDEELSAALIPPPVSSGFGGGSSGG
                     GSSCSSGGSSCSSGSSGGGGCGGGGCGG"
     gene            complement(6816..7658)
                     /locus_tag="AORI_RS54175"
                     /old_locus_tag="AORI_2636"
     CDS             complement(6816..7658)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="AORI_RS54175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_2636"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_016333000.1"
                     /transl_table=11
                     /translation="MNEPWGISGPVFAWLYGCLAVLPFLVAGLWAQSLRRRAADEWTPP
                     GPYHLAALAGGTERVADVVVATMVAGEQVRLDSQGRLTVLRAASGDPLVRAATDLVPRV
                     GAGGHYRLKKLLSRSAAVQEVRNDLQLSGLQVDERRRRVGWLAALIVYLPVLALGLARF
                     ANAIPLGRPAGVLAALLLAVVAAIAGTALLLRPSRTVLPTVAGLRVVGAARRDPLLVPG
                     VAGAVALGGLAAYPDPVMADMLVRTGDATAKESAGSSGGGSGCAGGSSCGGGGGGCGG"
     gene            complement(7728..8150)
                     /gene="msrB"
                     /locus_tag="AORI_RS54180"
                     /old_locus_tag="AORI_2637"
     CDS             complement(7728..8150)
                     /EC_number="1.8.4.12"
                     /GO_function="GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333001.1"
                     /locus_tag="AORI_RS54180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_2637"
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="WP_016333001.1"
                     /transl_table=11
                     /translation="MKPVVGATPRVVKSEQEWREQLSPDEYAVLRQAGTERPFTGEYTD
                     EKTTGVYQCRACGAELFRSETKFDSHCGWPSFYDPADTDAVLLREDTTMGMRRIEVLCK
                     SCHSHLGHVFEGEGYATPTDQRYCINSISLKLVPES"
     gene            complement(8170..8475)
                     /locus_tag="AORI_RS54185"
                     /old_locus_tag="AORI_2638"
     CDS             complement(8170..8475)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033523.1"
                     /locus_tag="AORI_RS54185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_2638"
                     /product="hypothetical protein"
                     /protein_id="WP_016333002.1"
                     /transl_table=11
                     /translation="MADSGPFGPGQNPRFQEDLVGLDPYDPEARAFAEHLDRIERCGPN
                     FTVEACLDGVSDFADSSNRLGGLRWWVSALVVVLIVLGVMVTAWDIIGRAIAWLAG"
     misc_feature    8538..8551
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.74"
                     /tool="antismash"
     gene            complement(8549..10198)
                     /locus_tag="AORI_RS54190"
                     /old_locus_tag="AORI_2639"
     CDS             complement(8549..10198)
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157015.1"
                     /locus_tag="AORI_RS54190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AORI_2639"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_267283981.1"
                     /sec_met_domain="Pkinase (E-value: 2e-53, bitscore: 180.3,
                     seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIAGHYRLVEQIGSGAMGVVWRAIDVRLERSVAVKQILPQPGVSE
                     AERDNMRQRAMREAKNAARFQHPNAIVVFDIAEHGGDPCLVMEYLNGPSLSAIISEEGT
                     LPLGRVAKIGEQVASALVAAHRAGIVHRDVKPGNILIDEAGTAKITDFGISRAAGDMTL
                     TQTGLIGGTPAYLAPELARGSDPVPSSDVFALGATLYQAIEGQTPYGNSSNQLALLYAA
                     ANGQINPPQQAGAATALLMSLLRTEPTERPTMAEARERLAALAANESGAGTAASPRLFG
                     AGGGRQPAVPETPGHGLPGGDRPPWQRTDKPVPSAAAPPQPTKTHTPTAAFIPMRSPSA
                     PPNTPPRPVAAMSPPPSAAAPMRPSPAKPTDAKRKAVLFAGAGAAVVVIAVIVFLILNS
                     SNGANTQAGGNTGVNPPPGTSAPKSSAPSSSRPSPAGLGKTPSEKITSYSSVGSFVLDF
                     FDTGKIGTSASWNMLTDAAKEVYGSEQAFAEYWSTHKVTSADTARADKGGGNADGSIDM
                     NITLNKGTRPALRVVQVGGQYYIDADTRVLGN"
ORIGIN
        1 agatcgggta ctgacgtcat cgcggtcacc aaccgactgt aagacgcgat cgtccggttc
       61 ggggcgcagg cgcgccgaga gtaaggcctc cgagcgcaca tggcaccatg aaccgtgatg
      121 tctccttcag ttgcttctcc cgcgcagacc gtcccggcgg cccgtcgcgc cctgcccggt
      181 gcgccgttcc tggccgccgc gcgcggcgaa cgcccggctc acacccccgt gtggttcatg
      241 cgccaggcgg gccggtcact gcccgaatac cgggcgctgc gcgaaggcac ctcgatgttc
      301 gaagcgtgct tcgaccccga gatgctcgcc gagatcacgc tccagccgat ccgccgtcac
      361 ggcgtcgacg cggccatcct cttcagtgac atcgtcgtcc cgctcaaggc ggcgggcctc
      421 gacatcgaca tcgtcgcggg caccgggccg gtcgtcgccg agccgatccg cgacgccgcg
      481 ggcgtgcgcg cgctgccggt gctggagccc gagcaggtgg agcgggtcgc cgagggtgtc
      541 cggctgctgg tcgggtcgct gggcgagacg ccgctgatcg ggttcgccgg tgcgccgttc
      601 acgctggcct cgtacctcat cgagggcggg ccgagccgca accacgagca caccaaggcg
      661 ctcatgcact ccgagcccgg gctgtggcac gagctggcgg ggcgcctggc ggacatggcg
      721 atcaccttcc tctccgcgca gctcgacgcc ggggccgacg cgatccagct gttcgactcg
      781 tgggcgggcg cgctctcgga gcgggactac cgcgagttcg tccttccgca ttcggcgaag
      841 gtgctgtccg cggtcgcgcg ctacggcgtg ccccggatcc acttcggcgt cggcaccggc
      901 gagttgctgg tggccatgcg cgacgcgggt gccgacgtgg tcggcgtcga ctggcgcatc
      961 ccgctcgacg aagccgtgcg gcggctcggc ggcggcgtgg tccagggcaa cctcgacccg
     1021 gcgctcctgc acgggtcctg gccggtcatc gaggccgagg tgcggcgcat cctcgccgag
     1081 ggccgcgcgg ccgacggcca catcttcaac ctcggccacg gcgtgctgcc cggcgtcgac
     1141 ccggacgtac tgacgcgcgt ggtcgggctg gtgcacgagc ttcagccgtg aagacggtcg
     1201 ccgtcgtcgg tggcgggatc tcgggactga ccgcggcgta ccggctgcgg accctgctcg
     1261 gcgaggacgc caggatcgtc gtcttcgagg cgaccgggtc gctcggcggg aaactccgca
     1321 cggtcgaact cgccggtgag cgctacgacg tcggcgcgga ggccttcctc gcccgccgtc
     1381 ccgaagcgct cgcgctggtg cgtgaggtcg ggctgggcga aagcctcgtc catcccacca
     1441 aggcccgcgc gaagatccac gccggcggtt ccgtactcgg cctgccgccg ggcacggtca
     1501 tgggtgttcc ggcgtcggcc ggcgcggtcg cggggatgct gtcggagaag ggccgcgcgc
     1561 tggtcgaggc cgaggcgtcg ctgcctccgc tggaactgcc cgcggaagat gtgccgctcg
     1621 gcccgctgct gcgcgaacgg ttcggcgacg aactcgtcga ccggctcgtc gatccgctgc
     1681 tcggcggggt ctacgccggg ggagcggacg ggctcggcct gcgcgcgacc atgcccggcc
     1741 tcgcctccgc gatcgatcgc ggcgcgactt cgctcaccga aggcgccgcc tcccgcctgc
     1801 ccgcctcgcc gagtacggcg ccggtcttcg gcaccctgct gggcgggctc gggacgctga
     1861 tcgctcggct cgcggagctg tcccgcgccg agatccgcac cgggaccacg gtgacggcgc
     1921 tcgaccgcac ccccaccggc tggaaggtcg acgacctcga cgccgacgcc gtccttctcg
     1981 ccgtcccggc gccctcggcg cggcggctgc tcgacggcgt cgcgggagtg gcctcgtcgg
     2041 tgttcgcgaa ggtcgaactc gcgtcgatgg cggtggtcgc gctcgcgctc ccgccgggga
     2101 ccgcgctgcc ggagtcgtcc ggggtgctga tcggcgccgg ggaacgggac gccgccggga
     2161 agccgttcgc cgccaaggcg ttcaccttct cgtcccgcaa gtgggcgcag tacggcgaag
     2221 gtccggtgct ggtgcgcggc tcggtcggcc gcttcggcga accgggcgcg ctgcacgccg
     2281 acgacgacga actcgtgcgc gtggtccgcg acgatctcgc ccggctgacc ggggtcaccg
     2341 ccgagccgat cgacatcctc gtgacccgat ggggtggcgg actcccgcag tacggcaccg
     2401 ggcacctcgt ccgcgtcgag cggatcgaac gtgccgtcgc cgccgttccc gggctcgccg
     2461 tggctggcgc gacactgcac ggcgtcgggc ttccggcctg catcgccacc gccgacgccg
     2521 ccgcccgccg catcgccggg atcacaccgc cccacgaagg ctcctgagca ccggcagtgc
     2581 caagatggcg gtatggcgcg gctgaactac aacgagctca atgacaccat ccgctacacc
     2641 gcctggtcgg tcttccgggc cgagcagggc aagctcggcg aagaccgggc gaaggcgacc
     2701 gcggagacca ccgagtacct cgacgcgctg gaagccaagg gcgtcgtcgt ccgcggtgtc
     2761 tacgacctct ccgggctgcg cgccgacgcc gactacatga tctggtggca cgccgaggag
     2821 atcgagcagg tccaggcggc gtacagcggc ttccggcaca ccccgctcgg ccgcgcgtcg
     2881 gcaccggtgt ggagccaggt cgcgctgcac cgcccggcgg agttcaacaa gagccacatc
     2941 ccggcgttcc tcgccggtga agaggcccgc aagttcatct gcgtgtaccc gttcgtgcgc
     3001 tcgtacgagt ggtacctgct gccggacgac gagcgccgca agatgctcgc cgaccacggc
     3061 aaggaggccc gcgactaccc ggacgtccgc gccaacacgg tcgcgtcgtt cgcgctgggc
     3121 gactacgagt ggatcctcgc cttcgaggcc gacgaactgc accgcatcgt cgacctgatg
     3181 cgtcacctgc gcggcaccga ggcacgcctc cacgtgcgcg aggagatccc gttctacacc
     3241 gggacgaagg tggcccccgc cgagctgatc gcgctcctgc cctagtgaag gcctccgtgg
     3301 acacccgcgt ctggaccacg ctcaccggcg acaccctcgc cgaagacgcc gtcgagatca
     3361 ccggccccga gtcggtgctg cccgggacct tccgggtcga ggaggcggcg acgacgagta
     3421 tcgccgcggc gacgctcgcg gccggggaac tgttgcggct gagggggatc gagccgggca
     3481 aggtctcggt cgacacccgg cacgcagcgg cggcgttcca cagcgagcag tacctccgcg
     3541 tcgacgggga gccggtgccg ttcagcgcgc cgctgtccgg cgactaccgc gcgaacgacg
     3601 gctgggtgcg gctgcactgc aactacccgc ggcacgtgtc cgccgtgtgc tggggtctcg
     3661 gcgtacccgc gacgcgggcg gcgttcgaga aggtcgtggc ggccaagtcg aagtacgagg
     3721 tcgagtcggc cgtggtcggc gcgggcggtg ccgcggccgt gctgcgcacc cgtgacgagt
     3781 ggctggcgca caagcaaggg caggcgtcga gcgcgttgcc ggtggccgac atccggccgc
     3841 tcgggacgtc ggagccggtg cggctgttcg aatccggccg gccgctgggc ggggtgcgga
     3901 tcctggacct gacccatgtc atcgccgggc cggtcgcggg ccgcgtgctc gccgcgcacg
     3961 gggcgaccgt catgcacatc ggttcggcgc agctgccgac cgtgccgccg ctggccgtcg
     4021 acaccggcat ggggaagctc tcggcgtacg tcgacctgga gaccgaagcc ggacgctcgc
     4081 ggctgcgcaa gctgatcgcc cgcgccgacg tcgtgctgca gggtttccgg ccgggttcgt
     4141 tgacggccaa gggtttctcg ccggaagcga tcgccgagct ccggccgggc atcgtcgtcg
     4201 cggatctgtc cgcgtacggc tgggaagggc cgtgggcacg caggcgcggg ttcgacagcc
     4261 tggtgcagat ggcgtcgggc atcgccgagg agggcggcag gaccgccggg ctcgacggcc
     4321 ccggaccgct tccggtgcag gcgctcgacc acgcgaccgg ctggctcacc gcggcggcga
     4381 tcatgaccgc cgtccgtcag gcggccaccg aaggcggcag ccggcatgtc cgtacctcgc
     4441 tcgcggggac cggggagtgg ctgaactcct tgggccgtaa ggaagccggg cctgccgggt
     4501 tcgacggcgg cgagtggctc gaagacgtcg acggccagct cggccgggtc acccgggtca
     4561 agatggcggg caccctgccg ggctcgaatc cacaatggac ggaagcgacg cacgtaccgg
     4621 ggagcgaccg cccggtttgg tgaaaccgca ttcagaggac taaatgcggc ggagccagct
     4681 cgcgaaccgg ccccggggtt tcgggacttc gccgcgctcc acgagccacg tgccgaaagc
     4741 gtccgcgtag gcccgcgcgc ggatcgtgtc ggtcttcggg tgagccgtcg cgtactcgcg
     4801 gaagagcgcg gcgaaccggt caccgagggc ctcggcgagg tccggccgaa ggtaggcggc
     4861 cagcctgcat tgcttgttcc gcatgacgtt ggcctcgatg cggagcctgt ccgcgtcgaa
     4921 gcccgccggg gcgtcgccac cggcgagaag cgccttgagc agctccgcct gctgcccggc
     4981 gagccgctcc cggggcgacg tcacggcgag accgcgtctc gcagcgcggc cagctccgcc
     5041 gcgagttcgg cgtcggacgg gtagtcgtcg tcgcgttcca gcaggacgcc gggcggttcg
     5101 gtgcgccgcc gcagttcgcg cagcaggtcc agcacttccg gcaccaccgg atgggcgtgc
     5161 gtgtcgtggt agacgccgtc ccgttcgacc ccgcccgcca tatgggtgta cgccaggcgc
     5221 tcccacggga tctcgtcgag gaaccgttcg ggatcggtgc cgaggttgcg ggcgttggcg
     5281 tacaggttgg ccacgtcgac gatcagcagg cagccggtcc ggtcggtgag ctcccgcagg
     5341 aactccgact cggtgtactc cgcgtccggc cattccagca ccgccgcgat gttctccagc
     5401 gcgaacggga cctccacgat ggactgcgtc agtttgacgt tgtccaccag gacgtcgagc
     5461 gcctcccggg tgcgggggag cggcatcaga tggcccgagt cgaggccgcc cgcgcgcacg
     5521 aagcacacgt ggtcgctggc cagcggtgcg ccgagctcgc gggtgatctc cgcgagatgc
     5581 tcgacgcgcg cggtgtccag cggctcggcc ccgcccagcg acagcgagac ggcgtgcggc
     5641 aggaccggca ttcccttgtc gcgcaacgcg agcagggttt ccggaagatg cgcggcgtgc
     5701 aggttctcgg cgacgacctc gacccagtcg acccctggca accgcgcgat cgaaaggtcg
     5761 agctcgtgcc gccagccgat gccgatcccg agcgtgccgg acccgtcagc cgccacagcc
     5821 gcctccaccg caaccaccgc cgccgctgct tccgctgctg cacgagcttc cgccgctgct
     5881 gcacgaagag ccgccgccgg acgagccgcc gccgaaaccg ctggagaccg gcggcgggat
     5941 caaggccgcg ctgagctcct cgtcggggta cgaggcgagg ccgccgaacg cgaccgccgc
     6001 cgcggcgccg cccagcaccg cgccgccgac gtatcccggt ccgttcggga tgccgcgttt
     6061 gcgctcccgt ccgtgggcgg acctggcctt gtggaggacc cgggcgccct cgctggtcgg
     6121 acgcgggccc ggccgcttgt tcttgagcgc acgggagatc agcgtcgcga acacggccgc
     6181 gatcagcatc gggatcagga atccgaccgg gcggtcgccg gcgatccccg cgatcaggcg
     6241 caccacaccg aggacgagta cggcgaggta caggaagtac acgacccggc ggatccgtgg
     6301 cgtctggtcg acgacgacca gcccccgtgc ctccagatcc ttggcgagcg cctgcatcgg
     6361 gcccgacatc cggagccggt cacggatgcc gcgggtcgtg gcgttgtagc ccggcctggc
     6421 gcccttggcg acggcctttt cgatcgggtc ggcgggctcg ggaccggtga gctggagctg
     6481 cttcgtactg ctgacccgca aggtcccgcg gtcgaccagc gtcgcgatgg ccgtgtcgac
     6541 cacccggtcc ggtccgccgg cgagataggc gaactcgtac accgtcggct gcgggccgct
     6601 cagttcggcg cgcggcgctt tgccgcccag tttggccatg atcgtccacg cgaaaccggc
     6661 gaccgcgggg aacacgatca gggcgccata gatcgccagg aacgtgggtc cggggatgcc
     6721 ccaagtgtct tccatcgcac gggcctttca cgagcaggtg cacttaccgt agtcggacgc
     6781 ttttccgtcc ggtcgtggtt ccgtgattcc agggttcagc cgccgcaacc acctccgccg
     6841 ccaccgcagg acgaaccgcc cgcacagccg cttcccccgc ccgacgaacc cgccgattcc
     6901 ttagcggtgg cgtcgccggt cctgaccagc atgtccgcca tcaccggatc gggatacgcc
     6961 gccagcccgc cgagcgccac cgcgcccgcc actccgggga ccagaagcgg atcccgccgt
     7021 gccgcgccga cgacgcgcag tccggccacg gtcggcagga cggtacggct cggccgcagg
     7081 agaagggcgg tcccggcgat ggccgcgacc accgcgagga ggagagccgc cagcacgccg
     7141 gccgggcggc ccagtggaat ggcgttcgcg aacctcgcca gaccgagcgc gagcaccggg
     7201 agatagacga tcaaggccgc cagccatccc acgcgacggc gacgttcgtc cacttgcagg
     7261 ccgctcagct gaagatcgtt gcgcacttcc tggacggcgg cgctccggct cagcagcttc
     7321 ttcaaccggt aatgcccgcc ggcgcccact cgcgggacga ggtcggtggc ggcgcgtacg
     7381 agcggatctc ccgatgcggc ccggaggacg gtgagccgtc cctggctgtc cagccgcacc
     7441 tgttcaccgg cgaccatggt ggccaccacg acatcggcga cccgttcggt gccaccggcc
     7501 agcgcggcga ggtgatacgg gccaggcggc gtccattcgt cggcggcacg acgccgcagc
     7561 gactgtgccc atagaccggc gacgagaaac ggcaggacgg cgaggcagcc gtacagccac
     7621 gcgaaaaccg gtccggagat cccccagggt tcgttcatac gacaccgccc tccccgacgt
     7681 gtttcctcgt gaacgcgtcg aagagggcgg tggttcgctt cggtcggtta cgactcggga
     7741 acgagcttca gcgagatcga gttgatgcag taccgctgat ccgtcggcgt ggcgtagccc
     7801 tcgccttcga agacgtgccc gaggtggctg tggcacgact tgcacagcac ctcgatccgg
     7861 cgcatcccca tggtggtgtc ctcgcgcagg aggacggcgt cggtgtcggc ggggtcgtag
     7921 aacgacggcc agccgcagtg gctgtcgaat ttcgtttcgc tgcggaacag ttcggcgcca
     7981 caggctcggc actggtagac accggtggtc ttctcatcgg tgtactcgcc ggtgaagggg
     8041 cgctcggtgc ccgcctggcg aaggacggcg tactcgtcgg ggctgagctg ctcccgccat
     8101 tcctgctcgg acttgaccac gcgcggcgtg gctccgacaa cgggtttcat gcctttcacc
     8161 ggttccacgt taccccgcga gccacgcgat ggcccggccg atgatgtccc atgcggtgac
     8221 catcacgccg agcacgatga ggaccacgac cagtgccgaa acccaccagc gcagcccgcc
     8281 gagacggttg ctggagtcgg cgaaatcgga gacaccgtcg aggcaggcct ccacggtgaa
     8341 gttcgggccg cagcgttcga tgcggtccag atgctcggcg aaggcccggg cttcgggatc
     8401 gtacgggtcg aggcctacca ggtcctcctg gaagcgagga ttctggccag gtccgaaagg
     8461 tccgctatcg gccataccgg caacagtaag ccacgatcgg cgattgtcct accaccagcg
     8521 aggcgggtct caccgccgcc acgctgcgtc agttgccgag tactcgcgtg tcggcgtcga
     8581 tgtagtactg cccaccgacc tggaccacgc gcagtgccgg gcgtgttccc ttgttgagcg
     8641 tgatgttcat gtcgatcgag ccgtcggcgt tgccgccgcc cttgtccgcg cgggcggtgt
     8701 cggcgctggt gaccttgtgg gtgctccagt actcggcgaa agcctgctcc gaaccgtaga
     8761 cctctttcgc ggcgtccgtg agcatgttcc agctcgcgga cgtgccgatc ttgccggtgt
     8821 cgaagaagtc gaggacgaag ctgcccacgc tggagtagct cgtgatcttt tcgctgggcg
     8881 tcttgcccag tcccgcgggc gaggggcgcg acgaagacgg ggcgctcgac ttgggcgccg
     8941 aggtgcccgg cggcggattc accccggtgt tgcccccggc ctgggtgttg gccccgttgc
     9001 tcgagttcag gatcaggaac acgatcacgg cgatcaccac gaccgcggca ccggcgcccg
     9061 cgaacaggac ggccttgcgc ttggcgtcgg tcggcttggc gggcgacggc cgcatcgggg
     9121 cggccgccga cggcggcggg ctcatcgcgg cgaccgggcg cggcggggtg ttcggcgggg
     9181 cggacggcga gcgcatcggg atgaacgcgg ccgtcggcgt gtgcgtcttg gtgggttgcg
     9241 ggggagccgc tgcggagggg accggcttgt cggtccgctg ccacggcggg cggtctccgc
     9301 ccgggaggcc gtgtccgggg gtttcgggca cggcgggctg tctgccgccg cccgctccga
     9361 acagccgcgg cgaggcggcc gtgcccgcgc cggattcgtt cgccgccagc gcggcgaggc
     9421 gctccctggc ctcggccatg gtcgggcgct cggtcggctc ggtgcgcagc aggctcatca
     9481 gcagcgccgt cgcggcgccc gcctgctgcg gcgggttgat ctgaccgttc gcggccgcgt
     9541 acagcagcgc gagctggttg gagctgttcc cgtacggggt ctggccctcg atcgcctggt
     9601 agagcgtcgc gccgagcgcg aagacgtcgg agctgggcac cggatccgag ccgcgcgcca
     9661 gctcgggcgc gaggtaagcc ggggtgccgc cgatcaggcc ggtctgggtg agcgtcatgt
     9721 cgccggccgc gcgcgagatg ccgaagtcgg tgatcttcgc ggtgccagcc tcgtcgatca
     9781 ggatgttgcc cggtttgacg tcgcggtgca cgatcccggc gcggtgcgcg gcgaccagcg
     9841 ccgacgcgac ctgctcgccg atcttcgcga cgcgccccag cggcagcgtg ccctcctcgg
     9901 agatgatcgc cgagaggctc gggccgttca ggtactccat caccaggcag gggtcgccgc
     9961 cgtgctcggc gatgtcgaac accacgatcg cgttcggatg ctggaaccgg gccgcgttct
    10021 tcgcctcgcg catggcgcgc tggcgcatgt tgtcgcgttc ggcctcggag acacccggct
    10081 gcgggaggat ctgcttgacc gccaccgagc gctcgaggcg gacatcgatg gcacgccaga
    10141 ccacccccat ggcaccgcta ccaatttgct cgaccaggcg atagtgccca gcgatcagct
    10201 gaccggtgtc gatggcgact gctcctgaca cattctccgg gtgatggtgg cgctacgcgg
    10261 cctgacaggt cccgcgcacc cgatcgagtg tagcgggcgg cggtttcggc tccccgtcag
    10321 ctcgccggtg cggaggcggg caattcttcc tcgccgctgg gttcgggtat cggacgggcc
    10381 ttgcgccgga agaccttgac cagcccgatg gcccccgcgg cggcgaacac ggcgtaacag
    10441 atcagcaggg cgggaggtgt ttccccggtc agcgcgtcgc cgaggacgac gaccgcgacc
    10501 gtccccggca ggctgcctag cgcggtcccg atcagatacg gggcgaaacg gacggatgac
    10561 actccgcaca ggtaactgaa cggagcgaac ggcaccatcg ggatcagccg taaggaggtg
    10621 accgccagta ccccgccgtc cgaaagccgg tcgttgaccg cgcggacctt cgcccggtgc
    10681 aggtgccggg tgaccagatc gcggccgagt gccctcgaaa gagcgaacga gagcgcggac
    10741 gcgaccatgg tcgccaggat gccgacgagg atgcccgtcg tggcgccgag cagcagcccg
    10801 cccgccaggt tgaaca
//
