LOCUS       NZ_KB976671            23908 bp    DNA     linear   CON 01-APR-2024
DEFINITION  Bacillus toyonensis strain HuA2-3 acrHg-supercont1.9, whole genome
            shotgun sequence.
ACCESSION   NZ_KB976671
VERSION     NZ_KB976671.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus toyonensis
  ORGANISM  Bacillus toyonensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 4008403)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C.,
            Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T.,
            Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     The Genome Sequence of Bacillus cereus HuA2-3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4008403)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C.,
            Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T.,
            Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2012) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_000399405.1
            Current Name                      :: Bacillus toyonensis
            Previous Name                     :: Bacillus cereus HuA2-3
            Date Updated                      :: 2022-03-10
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_000496285.1
            A1 Name                           :: Bacillus toyonensis
            A1 ANI                            :: 99.4201%
            A1 Query Coverage                 :: 83.75%
            A1 Subject Coverage               :: 94.68%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_006094295.1
            A2 Name                           :: Bacillus cereus
            A2 ANI                            :: 91.7421%
            A2 Query Coverage                 :: 77.41%
            A2 Subject Coverage               :: 80.96%
            ##Taxonomic-Update-Statistics-END##
            ##Genome-Assembly-Data-START##
            Assembly Method                   :: allpaths v. R41865
            Assembly Name                     :: Baci_cere_HuA2-3_V1
            Genome Coverage                   :: 108.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000399405.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 01:17:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,841
            CDSs (total)                      :: 5,738
            Genes (coding)                    :: 5,542
            CDSs (with protein)               :: 5,542
            Genes (RNA)                       :: 103
            rRNAs                             :: 6, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 3, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 81 of 196
            Pseudo Genes (incomplete)         :: 115 of 196
            Pseudo Genes (internal stop)      :: 62 of 196
            Pseudo Genes (multiple problems)  :: 52 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:43:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2777675
            Orig. end                         :: 2801583
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB976671.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            The organism was changed from 'Bacillus cereus HuA2-3' to 'Bacillus
            toyonensis' in March 2022.
            Please be aware that the annotation is done automatically with
            little or no manual curation.
FEATURES             Location/Qualifiers
     region          1..23908
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="9"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..23908
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..23908
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:13908](-)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..13908)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            complement(555..1493)
                     /locus_tag="IG5_RS22100"
                     /old_locus_tag="IG5_02815"
     CDS             complement(555..1493)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913375.1"
                     /locus_tag="IG5_RS22100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02815"
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="WP_000915069.1"
                     /transl_table=11
                     /translation="MLKKVFAMSTTVVLAAGLLSGCGTKESSASKNEDTKKGYVPKTLN
                     VQFVPSQNADTLEAKAKPLENLLSDKLNIPVKVSISTNYNTIVEAMASKQVDVGFLPPT
                     AYVLAHEKKAANVILQAQRFGVDDETGAPTKDLVDFYKSEFVVKKDSNINSVKELKGKK
                     IGYQDVTSSAGYVWPAAVLLKEGVDPLKDVKPVTLKGHDQSLIALLNGDVDAAVVFQDA
                     RNIVKKDYPNIFDQTKIVKFTEKIPNDTISVRSDLDAEWSKKLQDAFIEIGKNEEGHKI
                     IKEVYSHEGYVKSDDSKFDIVRDYGKKVKTQ"
     misc_feature    1514..1530
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: medium, score: 28.44"
                     /tool="antismash"
     gene            1679..3262
                     /locus_tag="IG5_RS22105"
                     /old_locus_tag="IG5_02816"
     CDS             1679..3262
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1283: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002199635.1"
                     /locus_tag="IG5_RS22105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02816"
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="WP_001075251.1"
                     /transl_table=11
                     /translation="MNQNQIVTLNILLTSDVHGTVYPIHYQDNSKAEIGLAKISTLIKK
                     ERSKNTNVLLVDNGDFIQGTPLTYHYATYEKDKKNPMSLLANYLNYDAAIIGNHEFNYG
                     MDILNHAVETANFPYLSANILDKNIKEPYFGKPYIIKHIESNIKIAILGATTHYIPNWE
                     QAHHIKDLLFEDALETIKKWVPYIRKHEQPDLLVVAYHGGFERDFQTGVPTEVLTGENQ
                     GYAMYDEIDGIDILLTGHQHREIAQTLDNGVTILQTGCNGHSVGKVKVIFEKTEQKWVK
                     KNSYAQLLPVQGIEADKDVLSLVADYEEKTQKWLDQPIGIIHGDMRISDAMQTRLQDHP
                     FIEFINKIQMDIANVSISCTSLFHNTSPGFPKHVTMRDIVSNYIYPNTLKVIRVTGADI
                     KDALELSASYFTLNKVGDIIVNPAFIEPKPAHYNYDMWEGISYVLNISKPIGERVESLQ
                     YKGATLDINEEYDVVMNNYRANGGGNFFMFQNKPVIKDIPTDMSELIANYILKHKRIEA
                     TRNNNWKVIK"
     gene            complement(3293..3532)
                     /locus_tag="IG5_RS22110"
                     /old_locus_tag="IG5_02817"
     CDS             complement(3293..3532)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002172959.1"
                     /locus_tag="IG5_RS22110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02817"
                     /product="DUF896 domain-containing protein"
                     /protein_id="WP_000931861.1"
                     /transl_table=11
                     /translation="MLNHELVERINFLAKKAKAEGLTEEEQRERQSLREQYLKGFRQNM
                     LNELKGIKVVNEEGTDVTPAKLKALKKQDNAKLN"
     gene            complement(3578..4234)
                     /locus_tag="IG5_RS22115"
                     /old_locus_tag="IG5_02818"
     CDS             complement(3578..4234)
                     /GO_function="GO:0000150 - DNA strand exchange activity
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016111193.1"
                     /locus_tag="IG5_RS22115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02818"
                     /product="recombinase family protein"
                     /protein_id="WP_000991792.1"
                     /transl_table=11
                     /translation="MNAIVYARVSTTKEAQETSLLRQKDELLHLAERYEMNVTKVIEEQ
                     ASGYTIERDGILEVLDTIRDEQVDALLIQDETRLGRGNAKIALMHCLHKEGIKVYTHTH
                     NGELQLSDSDSMVLDIISIVEEYQRKIHNLKIKRGMQRAVEKGYRPENNLKNRHLSVGR
                     EKKEVPIEEIVRLRKSELTFEEIAATLRGFGHNVSKATVHRRYVEYTKQLLDKEE"
     misc_feature    complement(4295..4308)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 22.42"
                     /tool="antismash"
     gene            4376..4996
                     /gene="lexA"
                     /locus_tag="IG5_RS22120"
                     /old_locus_tag="IG5_02819"
     CDS             4376..4996
                     /EC_number="3.4.21.88"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="lexA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413738.1"
                     /locus_tag="IG5_RS22120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02819"
                     /product="transcriptional repressor LexA"
                     /protein_id="WP_000413736.1"
                     /transl_table=11
                     /translation="MEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTVHG
                     HLSRLEEKGYIRRDPTKPRAIEILGDDRMDTETQSVIQVPIVGKVTAGLPITAVESVEE
                     HFPLPASIVSGSDQVFMLRISGDSMIEAGIFDGDLVVVRQQQSAYNGEIVVALTEDNEA
                     TVKRFYKEKDHFRLQPENSSLEPIILKQVSVIGKVIGVYRDLH"
     gene            complement(5111..5914)
                     /locus_tag="IG5_RS22125"
                     /old_locus_tag="IG5_02820"
     CDS             complement(5111..5914)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016512438.1"
                     /locus_tag="IG5_RS22125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02820"
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="WP_000976110.1"
                     /transl_table=11
                     /translation="MMKKILLVAIACIIIGVIGISQTYEKAVQTAEKGDKEDVIKNETL
                     KNIEVKLGAGDIVVQKSPDASFYVKQTGDVSKQEIRITEDGDTLKVIGERKKDVSFDFS
                     FYNFGFQTSTLTIFVPERSYQEIKVNSAAGQIEISDVKSDRVNVGTLAGEVDLKYVTAK
                     NVEGSTKAGEVNFKKVMGKVTAKTTSGDVYIMDHDPKYDVEASSTAGDVTITLSEKPKD
                     AVITGQTPGGDVTIFNEENKNVTIGNGSKKISGKTAAGDVTIETR"
     gene            complement(5915..6457)
                     /locus_tag="IG5_RS22130"
                     /old_locus_tag="IG5_02821"
     CDS             complement(5915..6457)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031379.1"
                     /locus_tag="IG5_RS22130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02821"
                     /product="DUF1700 domain-containing protein"
                     /protein_id="WP_001031393.1"
                     /transl_table=11
                     /translation="MNKEQFLRELSNQLRKLPEEERKDILFDYEEHFQFGIEEGKTELE
                     IIKGLGSPKVIAKELLAMYRFDEMKKDPSTSNVTRAVMAAIGLSLFNFIIVLGPLVAII
                     GFIFSFWVGGIASVVTPFFVIVKVFMGTFIWLDLFVSITFVGVGLLLCMIAYYSTKWFK
                     RLCVRYVIWNFKMIKGE"
     gene            complement(6450..6773)
                     /locus_tag="IG5_RS22135"
                     /old_locus_tag="IG5_02822"
     CDS             complement(6450..6773)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016105867.1"
                     /locus_tag="IG5_RS22135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02822"
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_001102631.1"
                     /transl_table=11
                     /translation="MNVQFKKGVLELCVLALVKRKDSYGYELVQQISNKFLISEGSVYP
                     LLRRLTKEGYFQTYLKESTEGPPRKYYQLTEQGETQLHLLVTEWSDFAKGVQEIIEGVN
                     WDE"
     gene            complement(7001..8800)
                     /locus_tag="IG5_RS22140"
                     /old_locus_tag="IG5_02823"
     CDS             complement(7001..8800)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016096316.1"
                     /locus_tag="IG5_RS22140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02823"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001106590.1"
                     /transl_table=11
                     /translation="MNYIIYFMKKLYSSAGKILYVNLFGMVLISLLEGIGIILLLPMIS
                     MTGIINTGEETSFIAPVYRILQNFPKTTSLISILGIYVLIVLSQNILQRNITLRDARTQ
                     QVFVRKLRVETYSMILKAKWRFFLEKRKTNLINILTTELARVSYGINLILQLLATILFT
                     FIQVGLAFLLSPQVTVFVLGFGLLFLVASRVFIKKARILGGKTSELAKDYLSGITDHFN
                     GIKDIKSNTLEGSRLEWFQSITKKMSSEQMEYMRIRSNSQLFYKISMAILIAFFIFLSV
                     NMFQAQTAQLLLIILIFSRLWPRFMTIQSNLEQLAASIPAFKSLWELQQECNEAVEMQD
                     IYMQKHIKPICIKQGIECKDVSFRYNKQEALYALQNINVQIPINSMTAIVGHSGAGKST
                     LIDVLMGLIQPEKGQVLIDGTPLTTDNLLSLRQAISYVPQDPFLFNASIRENLMMIDSN
                     VNEEQIWAALEFAAATEFVRMLPNGLDTLIGDRGVRLSGGERQRLVLARAILRKPSILV
                     LDEATSALDTENEVKIQSAIERLKGTMTIVVIAHRLSTIRNADQVIVLGKGEIVQKGEF
                     NQLAKERKGVFRNLLGSQMEVGS"
     gene            complement(8797..9999)
                     /locus_tag="IG5_RS22145"
                     /old_locus_tag="IG5_02824"
     CDS             complement(8797..9999)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_5"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409754.1"
                     /locus_tag="IG5_RS22145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02824"
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_000409751.1"
                     /sec_met_domain="NTP_transf_5 (E-value: 8.5e-64, bitscore:
                     213.4, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKDFGLNVEFMSKELKLLFAILKLKDDETMQTYSNEWFSDIDWE
                     LFLEQVVHHRVYPVIYQKLKQVDEEIIPSHIVQSLNRKYKKNTFQMLYLSAEMERINKI
                     CNENEIRMIFLKGPVLSQDLYGDISLRTSCDLDVLIPIQNLEKVEMLLLEQGYVKDDYI
                     QTILNDWKWRHHHITFFHPTKQIKLEIHWRLNPGPSKEPGFEELWKRKRKSTLTSNSVY
                     MLGKEDLFLFLVSHGARHGWSRLRWLVDIKKIVNQDLNWSEIHKLMKTYQMLHLGGQAL
                     ILSSQLLGVRMDERMMELVAKKHSKQLAQEALFYFKQMINLHTDPVPEEVLAYHSRHLF
                     SLMSNQQKTLFIMSQLYPYPIDAKTLPLPKFLHFLYFPLRPFLCIWRKTKKFALLLGEH
                     R"
     gene            complement(10001..10474)
                     /locus_tag="IG5_RS22150"
                     /old_locus_tag="IG5_02825"
     CDS             complement(10001..10474)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012261438.1"
                     /locus_tag="IG5_RS22150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02825"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_001028388.1"
                     /sec_met_domain="PF13471 (E-value: 7.9e-24, bitscore: 81.9,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIVKRLRVFLLLNMETKLLFLEAFIFLGWARVLKSITFSKVAPS
                     LGDYMNETSVAQIQQHEDTLKEVSEAISIMSRYTFWESQCLVKAIAGMKMLEKRDIEST
                     LYLGTAKDNSGALIAHAWLRSGSFYVTGSEGMEKFTVVGSFAKRLSENTIKGE"
     gene            complement(10511..10819)
                     /locus_tag="IG5_RS22155"
                     /old_locus_tag="IG5_02826"
     CDS             complement(10511..10819)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002128586.1"
                     /locus_tag="IG5_RS22155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02826"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_000260275.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 2.1e-26,
                     bitscore: 89.7, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 3.2e-15, bitscore: 54.1,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVHSRKISLKHNVVQSKGNIVSDMDGEKVMLNVQKGKYYNLGEI
                     GGEIWELIEKPIIINQLVTMLVSNYDIERKDCEEQVLSFLECLFQEGLIQIKDQPNR"
     aSDomain        complement(10532..10780)
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IG5_RS22155_Stand_Alone_Lasso_RRE.1"
                     /evalue="1.40E-28"
                     /identifier="RREFam002.1"
                     /label="IG5_RS22155"
                     /locus_tag="IG5_RS22155"
                     /protein_end="96"
                     /protein_start="13"
                     /score="89.7"
                     /tool="antismash"
                     /translation="VVQSKGNIVSDMDGEKVMLNVQKGKYYNLGEIGGEIWELIEKPII
                     INQLVTMLVSNYDIERKDCEEQVLSFLECLFQEGLIQI"
     gene            complement(10782..11753)
                     /locus_tag="IG5_RS22160"
                     /old_locus_tag="IG5_02827"
     CDS             complement(10782..11753)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108921.1"
                     /locus_tag="IG5_RS22160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02827"
                     /product="hypothetical protein"
                     /protein_id="WP_016091243.1"
                     /transl_table=11
                     /translation="MFNSMGNVLYQAFGLKIVSEIPLPELSQLNEKKEVADVQVEITDL
                     SEQWCKYSAEKQKTIVVEDNVVMFHVPQTAIFSIQAGEKITFSPMADAEEDKIRLYILG
                     TCMGALLMQRKTLPLHGSVIAIDGKAYALIGDSGAGKSTLASAFLNKGYQLLSDDVVAV
                     SLLNKNTAIVHPSYAQQKLWQESINELGMEVAQLSPLFERETKYAVPVNSQFFPDPLPL
                     AGVIELVKTQGEDIETTRIEGLARLYKLYRHTFRSSLISKLGLMEWHFETSTHIASGME
                     MFQVSRPTSRFTAHELVEVILNTIQKGSMVDGCPLEKNIIKT"
     gene            complement(11830..11955)
                     /locus_tag="IG5_RS30930"
                     /old_locus_tag="IG5_02828"
     CDS             complement(11830..11955)
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002172970.1"
                     /locus_tag="IG5_RS30930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02828"
                     /product="paeninodin family lasso peptide"
                     /protein_id="WP_000712621.1"
                     /transl_table=11
                     /translation="MKKEWKQPVLEVLNINMTMAGPGHKYPDAVQPDPDDPVKYS"
     CDS_motif       complement(11896..11955)
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MKKEWKQPVLEVLNINMTMA"
                     /locus_tag="IG5_RS30930_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       complement(11830..11895)
                     /prepeptide="core"
                     /locus_tag="IG5_RS30930_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="-15.80"
                     /molecular_weight="2264.4"
                     /monoisotopic_mass="2263.0"
                     /core_sequence="GPGHKYPDAVQPDPDDPVKYS"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MKKEWKQPVLEVLNINMTMA"
                     /leader_location="[11895:11955](-)"
     gene            complement(11971..13908)
                     /locus_tag="IG5_RS22170"
                     /old_locus_tag="IG5_02829"
     CDS             complement(11971..13908)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274520.1"
                     /locus_tag="IG5_RS22170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02829"
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /protein_id="WP_001274520.1"
                     /sec_met_domain="GATase_7 (E-value: 1.8e-19, bitscore:
                     67.8, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 4.3e-23, bitscore:
                     80.4, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAITGIIHFNNEPISIEHGTRLMSELQKYPADDIQIWHKENAFL
                     GCHAQWITPESVGEQLPFYNYEKQLAITADAIIDNRNELFEKLQVDYSDRKNMTDSELI
                     LLSYQKWGEAAPKYLVGDFAFMIWDEKKQILFGARDFSGSRTLYFYRGEEKLAFCTIIN
                     PLFTLPYIEKKLNEQWLAEFLAIPVNFESVDPQLTVYKYIEQVPPSHTILVKEGEVKFS
                     RYYTPTVGKMLNLKSNEEYEEAFREVYKSAVKARLRTHHQVGAHLSGGLDSGSVVSFAA
                     RDLRTENKKLHTYSYVPVEGFEDWTHKGRIADERPFIQSTVEHVGNIQDYYLELPERSP
                     LSEIDDWLETMEMPYKFFENTFWLSGVYEKASQQGIGVLLSGQRGNWTVSWGPILDYQA
                     MLLKKLHWIRFYRELYLYSRNIGVKKSRVFEVVRRKAFPFLHQLLFSGKQDANSVIINP
                     EFAREMNVFDRLREQEIDITGTAISNAYEMKREQFEKPYYWSINGTYETKLSLRYALWD
                     RDPTNDLRLIQFCLSVPEEQYVQNGLDRALIRRATKSFLPDKVRLNQRVRGIQGADGVY
                     RMAPFWNEFIEEVEELSVDPVISEFLNVEVIKKAISKICKEPRPEYAFDLDFRVLMRSL
                     IFYRFIKKAI"
     gene            complement(14200..15363)
                     /locus_tag="IG5_RS22175"
                     /old_locus_tag="IG5_02830"
     CDS             complement(14200..15363)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (42-168). E-value:
                     2.3e-08. Score: 25.5. Matches aSDomain:
                     nrpspksdomains_IG5_RS22175_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557690.1"
                     /locus_tag="IG5_RS22175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02830"
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="WP_000557691.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.2e-96,
                     bitscore: 322.0, seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.6e-06, bitscore:
                     25.5, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHTVQHKKRIYLSSPHMSGNEQKYIQNAFDTNWVAPLGPNVDGFE
                     RELASFVGVRGGTAVSSGTAAIHLALQLLGIQKGDTVFCSSFTFVASANPIVYLGAEPV
                     FIDSEPETWNMSPSALAHALYEANKMGNLPKAIILVHLYGQSAKLDEILSLCHQYNVPI
                     VEDAAESLGSTYKGKASGTFGKFGVYSFNGNKIITTSGGGMLVSNDVEALERARFLATQ
                     AKDPAPHYEHSEIGYNYRLSNILAGIGRGQLEVLEDRVRARRFVYKRYYEALSHMPGFY
                     FMPELEHTRPNRWLTTLTIDEKESGISIGKLLRILAEENIEARPMWKPLHMQSLFKEMK
                     YYPHSKNEDVSQYLFQSGICLPSGSNMLAEDQQRVIQSILKAVEWKN"
     aSDomain        complement(14860..15237)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IG5_RS22175_Aminotran_1_2.1"
                     /evalue="2.30E-08"
                     /label="IG5_RS22175_Aminotran_1_2.1"
                     /locus_tag="IG5_RS22175"
                     /protein_end="168"
                     /protein_start="42"
                     /score="25.5"
                     /tool="antismash"
                     /translation="GFERELASFVGVRGGTAVSSGTAAIHLALQLLGIQKGDTVFCSSF
                     TFVASANPIVYLGAEPVFIDSEPETWNMSPSALAHALYEANKMGNLPKAIILVHLYGQS
                     AKLDEILSLCHQYNVPIVEDAA"
     gene            complement(15394..16089)
                     /locus_tag="IG5_RS22180"
                     /old_locus_tag="IG5_02831"
     CDS             complement(15394..16089)
                     /EC_number="3.1.3.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016086741.1"
                     /locus_tag="IG5_RS22180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02831"
                     /product="HAD family hydrolase"
                     /protein_id="WP_000236583.1"
                     /sec_met_domain="HAD_2 (E-value: 6.7e-23, bitscore: 79.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVIQAIVFDMDDTLYKEKDYVVSGFKAVDDWIKDNYGKIGFYNIA
                     IRLFNSGERKFVFNKTLKKLDIDYDEKLISNLIEQYRFHKPDIQLLDEADWVLNNLVNT
                     VKIGLISDGYLVAQKRKINALKLKERVHSIILTDKLGKECWKPSKIPYEKISKELQVPH
                     EQCVYIGDNLSKDFITAKKLKWLTIHISREDGIYHNLIVEQAYKAHYTIDNLRRLSDIS
                     VLKHMFLDT"
     gene            complement(16091..17068)
                     /locus_tag="IG5_RS22185"
                     /old_locus_tag="IG5_02832"
     CDS             complement(16091..17068)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002165241.1"
                     /locus_tag="IG5_RS22185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02832"
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_000509383.1"
                     /sec_met_domain="ATP-grasp_3 (E-value: 5.1e-23, bitscore:
                     79.8, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGGNKMNILFTSSGRRVTLIKKFKEVFEQVGIKGKIFTADLKETA
                     PTIYFSDKHFIVPRVNEEGYINELLKICRSEMINLIIPLIDTELILMANNSEVFEGIGV
                     KVLVSSMELNKIANNKKYTYNFFVSNNIPTPRVYSDEEIGRKEYQFPLLIKPYDGSSSK
                     GVTKIMNEKELEFFKEYIPNAMIQEYVSGEEYTIDVMVDFYGNIKTIVPRLRIETRAGE
                     VSKGVTKKDFDIIQAAETVIKALPNPIGCITLQCFKKEDGQITFIEINPRFGGGIPLSI
                     EAGANFPLWTIKMCQGEVFIDKDFNWRENLTMLRYDEAVFMESI"
     gene            complement(17055..17660)
                     /locus_tag="IG5_RS22190"
                     /old_locus_tag="IG5_02833"
     CDS             complement(17055..17660)
                     /EC_number="2.7.8.-"
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bac_transf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1146: sugar transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002070010.1"
                     /locus_tag="IG5_RS22190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02833"
                     /product="sugar transferase"
                     /protein_id="WP_000825901.1"
                     /sec_met_domain="Bac_transf (E-value: 3.6e-68, bitscore:
                     226.5, seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRLLDIFISLLLLICFFFIILLVAIIVKTQIGSPILFRQQRPGL
                     HGKPFYLYKFRTMTNERDNSGELLPDYARLTKVGKFLRKYSLDELPQLINVLKGQLSLV
                     GPRPLLMEYMPLYSQEQAKRHNVKPGITGWAQINGRNSISWDEKFKLDVWYVENQSFLL
                     DLKILYLTFSKVLKSEGITNNKHVTMPVFKGGIEHGGE"
     gene            complement(18272..20506)
                     /locus_tag="IG5_RS22195"
                     /old_locus_tag="IG5_02834"
     CDS             complement(18272..20506)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269321.1"
                     /locus_tag="IG5_RS22195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02834"
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="WP_000269322.1"
                     /transl_table=11
                     /translation="MWRRRDNLGNLNDLDLVSKPPFHNDILTYDSTQLKWIPKSFEATN
                     STSIYVLELDRWDVKNDGTDAINTSQGINNALVWAAQQGYTEVVLPRGIYLIDKQTPIE
                     PPSHLTLNLNGSTLKMETNNLTGYAIISFRRNQVYSRITNGIIQGDRYTHDYSSGGTHE
                     AGYGIELGSFTPPADGGNNTRFVSLDNLDILDCTGDAITLNSTFGQISPFPTALASSFE
                     QGAINTTDGSLVSSTTKIRSNLQIDMNQVAIVKYGYFGLYGNGFGALGSEIKCDYYDVI
                     FYTSNNIFLSSKTNVQFFDEVEVPNGASYAKVVLHQGNVPAPANCLINVRVPSFSQYTY
                     IEKCNLHDCRRQGISVCGAKNVYIRDNDIHHIAGTNPQSGLDIEDGYDLNQYIYIERNN
                     FHDNKNYNIIVVNGKFIYISNNSIMNTITNAYVGLSINGGADRVIVTGNNIRLTKVSLL
                     GDVIFSNNYVYGAQVNVQGVYVNRSINISDNVFSNSKMIIDTPFPYAVKVDSCRFINDT
                     DKLNSLSSLYQWTLELKNEPQTISNCIFEGQDVLYLNYVPVGTVKSGWIFENIIFNNVK
                     PPTLVAGTYTNCFFKDVIFLGIASTGSTLELKGCKFFSIDRNNTLFTVNNLKAFKMIDC
                     HIEKPNGTVLNIQNVSDEIVLRGNTIKITNDTLQRAIIVLDAAFTGKQVIIQNNIINST
                     NLMQVGIDNRTTSSTPQVVIQNNVLNNAKITITGREFLQGNIVNGVIDSN"
     gene            complement(20845..22404)
                     /locus_tag="IG5_RS22200"
                     /old_locus_tag="IG5_02835"
     CDS             complement(20845..22404)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222557.1"
                     /locus_tag="IG5_RS22200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02835"
                     /product="hypothetical protein"
                     /protein_id="WP_001222558.1"
                     /sec_met_domain="Polysacc_synt (E-value: 1.7e-08, bitscore:
                     32.2, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIQNSLKNILFGLSGQIISIGMGFVVRTVFIFTLGIEYLGVDGL
                     FTSILLMFSLANLGFDTAIIYSLYRPLAEKDIYKIQALMNLYQKAYRLIGVVVLLIGLS
                     LLPFLDHLVNADTTIKDINIIYLLFLISSVSSYYFVYKQSIIIADQSNYIISKIHTLFM
                     IISNALQIVLLIIISDYIVVLVAQLTLKVIENVYIANKANKLYPFLKEKNNAKLSKTDR
                     REFFENLYSLLLYKISGVVINATDNIVISKFIGVIWVGVYSNYLLILNTLNTLLGYLFY
                     SITASVGNLNVKENAEKKYFIFRVINFLNFWIYGFCTVCLWNLINPFITIWLGEEYVFN
                     KYVLFFILLNFFTSGMQNTTTMFRETTGLFKKGKYRPIIAAIINIVVSIILAKQIGIAG
                     VLLGTVISRLCTYFWYDPYIIFKMIFKKSVKVYFIRYILFVVLVLFSIIATDFIGYIFH
                     INIFMDMAIRSILCLIIPNLIFWMVFKEAEEYRYFLNLTRILIKKIKPKILTRKFPNDN
                     YP"
     gene            complement(22423..23691)
                     /locus_tag="IG5_RS22205"
                     /old_locus_tag="IG5_02836"
     CDS             complement(22423..23691)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084457.1"
                     /locus_tag="IG5_RS22205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_02836"
                     /product="hypothetical protein"
                     /protein_id="WP_001084457.1"
                     /transl_table=11
                     /translation="MNSIILDKIVVRNNRVDYYFSIKGDLQKYFKTSNHMFLEYNYDVS
                     DISLSILAIPFVANVVPLVWITNSTIVINELDKSFYICLNNIKNAYQNMFPGVKFKGSM
                     VVGEIIDNVYIPELEAASLFSGGLDALTTFIRIKDKHPLLVTEYGWHENEIEGSKVWEA
                     DKENAVNFVKDNGLENIFIQSNYGTFIIAGNIDHDFREKLNDTWWHGLHHGLAIISAAI
                     PIAFKLKIKCIYIASSNSPLYQVTCASDPTVDNEIKYASGTVFHDGYELTRQEKIKVVV
                     DHYSASKEPVNIRVCFKNEENCCKCEKCLRTIMGIIAEGRDPREYGFNIPSNLYQYVKT
                     SLNNEVKFFTNTFIEMYWNLIQVRMKQNCDCITDKNLLNWFLDYDFKAQRRKALLKYRI
                     TKFFPILKRKISAKFSRVGTQNN"
ORIGIN
        1 acgttgctgt tgtcctcctg ataattcatc tgctcgtgca tatgcttttt ctaaaatatt
       61 taccctgttc aaagcctgaa atgcaagttc caaatcttcc ttcggaaaca agcctaacgt
      121 tgtacgtaat gtcgaatgat atccaacacg cccggctaat acattcttca gtaccgtgga
      181 tcgctttaca aggttaaagc tttgaaaaat cataccaata tctcggcgca tgcgtcgtaa
      241 ctctttgcct tttgcagcag taatagactc accttcaata ataatttctc cttctgtaat
      301 ctcatgaagg cgatttactg atctgaggag tgtagatttt ccagccccag atagcccgac
      361 cattacaaca aattcacctt tttgaatttt taaatttatg tttttcaatc ctttcgtacc
      421 atttggatac actttggaga cattttgaaa ctctatcaca catcttacct accttctatg
      481 tattatcaat aactgaaaaa cggcaaacta actgaaatca cacactttcc agttagtcgc
      541 catttccata tgtattattg tgttttcact ttctttccat agtcacgaac gatatcaaat
      601 ttactatcat cagatttcac atatccttcg tgtgagtata cctctttaat gattttgtgc
      661 ccttcttcat tctttcctat ttcaatgaaa gcatcttgta atttcttgct ccactccgca
      721 tctaaatcag aacgtacaga aatcgtatcg tttggaattt tctccgtaaa ttttactatt
      781 ttcgtttgat cgaatatatt cggataatct ttttttacaa tattacgagc atcttggaat
      841 acaacagctg catcaacatc accatttaaa agagcaataa gtgactgatc atgacctttt
      901 aatgtaacag gtttcacatc ctttagcgga tcgactccct cttttaacaa tacagctgca
      961 ggccatacat accctgctga tgatgttaca tcttgataac caattttttt accctttaat
     1021 tcttttacac tgttaatatt agaatctttc ttaacaacaa actctgattt ataaaaatct
     1081 actaagtcct ttgtgggagc tccagtttca tcatctaccc caaaacgttg cgcctgtaat
     1141 attacgtttg cagccttttt ctcatgcgct aacacatacg ctgttggagg taaaaatcct
     1201 acatctactt gttttgatgc catagcttct acaattgtat tgtaattcgt tgaaatacta
     1261 acttttaccg gaatgtttaa tttatcactt aatagatttt ccaatggttt tgcttttgcc
     1321 tctaacgtat ctgcattttg agaaggaaca aattgaacat ttaacgtctt cggtacataa
     1381 ccttttttcg tatcctcatt tttacttgca cttgattcct tcgttccaca gccacttaat
     1441 aaccccgctg ctagtacaac tgttgtgctc attgcaaaaa cttttttcaa catgtgaatg
     1501 cctccatttt tcgttgttat aaaataacct tccgtaaaca tgaagaaata tatcacatat
     1561 ttttacaaac aattacttat ttacagaatc tttacacatt ctatacattc tgattaaaat
     1621 tcttttcata tatttgatat gattaattga aagataacaa tagaaaaggt gaatggtatt
     1681 gaatcaaaat caaattgtaa ctttaaatat tttattaaca agcgatgtac atggtactgt
     1741 gtatccaatt cactaccaag ataactccaa agcagaaatt ggtttagcta aaatttctac
     1801 tcttataaaa aaagaacgtt ccaaaaatac aaacgttctt ttagttgata acggtgattt
     1861 catacaagga acacctttaa cttatcatta cgctacatac gaaaaagata agaaaaatcc
     1921 aatgtcctta ttggccaact atttaaatta tgatgctgca attatcggca atcatgaatt
     1981 taattatgga atggatatat taaatcatgc tgtagaaact gcaaacttcc cgtacttatc
     2041 agcaaacatt ttagataaaa acataaaaga accttatttc gggaaaccct atataataaa
     2101 acatattgaa tcaaatataa aaattgctat tttaggggca acaacacatt acatcccgaa
     2161 ttgggaacag gcccatcata ttaaagattt actatttgaa gatgcattag aaacaataaa
     2221 aaagtgggtt ccttacatcc gtaaacatga gcagcccgat ttactagtcg tagcctatca
     2281 cgggggattt gaacgcgatt ttcaaacagg agtaccgact gaagttttaa caggtgaaaa
     2341 ccaaggatac gcaatgtatg atgaaattga tggtatagat attttactaa caggccacca
     2401 acaccgagaa attgctcaaa ctttagataa tggtgtaaca attttacaga ccggatgcaa
     2461 tggacactcc gttggaaagg taaaagtaat attcgaaaaa acagaacaaa aatgggtaaa
     2521 gaaaaatagt tacgcacaat tattgcctgt gcaaggaatc gaggccgaca aagatgttct
     2581 ttccttggtc gccgattatg aagagaaaac acaaaaatgg cttgatcagc ctattggcat
     2641 cattcatgga gacatgcgaa tttcagatgc tatgcaaact cgtttgcaag accatccttt
     2701 tattgaattc ataaataaaa tccagatgga tatagctaac gtttctattt cttgtacaag
     2761 tttgtttcat aatacatctc caggattccc gaaacatgtc acaatgcggg atatcgtttc
     2821 caattatatt tacccaaaca cattaaaagt tattcgagtg actggagccg atataaaaga
     2881 tgctctcgaa ctttctgctt cttatttcac attaaataaa gttggagata taattgttaa
     2941 tcctgcattc atagaaccaa aacctgcgca ttataattac gatatgtggg aagggatttc
     3001 gtatgtatta aacatttcaa aaccaattgg tgaaagagta gagtctttac agtataaagg
     3061 cgctacactc gatataaatg aagaatatga cgttgttatg aataattatc gagcaaacgg
     3121 cggagggaac ttcttcatgt ttcaaaataa accagtaata aaagatattc caacagatat
     3181 gtctgaactt attgctaact atatattgaa gcataaaaga atagaagcaa cgagaaataa
     3241 caactggaaa gttattaaat aacaaataaa aaagatgggc ttcgcccatc ttttaattta
     3301 attttgcatt atcttgcttt tttaaagctt ttaatttcgc tggtgtaaca tctgtacctt
     3361 cttcgttaac gactttaatt cctttaagtt cgtttaacat attttgacga aatcctttta
     3421 aatattgttc tcgtagcgat tgacgctcac gttgttcttc ttcagttaaa ccttcagctt
     3481 ttgctttttt cgctaagaag ttaatgcgtt caacgagttc gtgattaagc atctcgaatc
     3541 ccccttctaa tcaaaactga ttattatcat acaatagtta ctcttcttta tcaagcaatt
     3601 gctttgtata ctctacgtat ctacgatgta cagtcgcttt tgaaacatta tgaccaaatc
     3661 cacgaagcgt tgccgcaatt tcttcgaaag tgagttcact cttgcgcaaa cgtacaattt
     3721 cctcaattgg cacttctttt ttctctcgcc ctacgcttaa atgacgattt ttcaaattat
     3781 tttctggccg atagcctttt tctaccgcgc gttgcatacc acgtttaatt ttcaagttat
     3841 gaatctttct ttgatattct tcaacaatac taattatatc taataccatc gaatcagaat
     3901 ccgacaattg taattcacca ttatgcgtat gtgtatatac ttttattccc tctttatgta
     3961 aacaatgcat taacgcaatc ttcgcattcc ctctaccaag acgtgtttca tcttgtatta
     4021 gaagtgcatc aacttgttca tctcgaattg tatctaatac ttctaatatg ccatcacgtt
     4081 caattgtata gccactagcc tgttcctcaa tcacttttgt cacattcatt tcatagcgct
     4141 cagctaaatg caataattca tccttttgac gcaataacga tgtttcttgt gcttcttttg
     4201 ttgtactcac acgtgcataa acaattgcat tcattttgaa cccttctttc tcgctatatt
     4261 ttctctctag tgtatcataa atctcccttt tgttcgaact tatgtttgta gaatgttttt
     4321 tcgcatgtta tacttattaa taagaaaaac gaaagaaaac gaggtgttag aaaacatgga
     4381 aaagttaacg aaacgccagc aagacattct cgactttatt aagctaaaag tacaagaaaa
     4441 aggatatcca ccttccgtac gtgaaatcgg tcaagcagtc ggcctcgctt ctagttctac
     4501 agtgcacgga catttatcac gactagaaga aaagggctat atccgacgcg atccaacaaa
     4561 accacgagca atcgaaattt taggcgatga ccgaatggat acagaaacac aatctgttat
     4621 tcaagttcca atcgtcggaa aagttactgc cggtttacca attacagcag tcgaaagtgt
     4681 agaagagcac ttcccacttc cagctagtat cgtttctgga tcagatcaag tatttatgtt
     4741 acgtatttct ggagatagca tgattgaagc tggtatcttt gatggagatt tagttgttgt
     4801 ccgccaacaa caatctgcat acaacggtga aattgtcgtt gccttaacag aggataacga
     4861 agcaactgtt aaacgtttct ataaagaaaa agaccacttc cgtctacagc cggaaaactc
     4921 ttcattagaa cctatcattt taaaacaagt gtcagttatt ggaaaagtaa ttggcgtata
     4981 tcgtgattta cattaaaaat aataaaagaa gataattccc ttatttatat agggaattat
     5041 cttcttttat atattggtag attttaattc attccaaaaa actcgaatga atctatcttc
     5101 tcatctatta ttaacgtgtt tctatagtaa catctccagc agccgttttg ccgctaatct
     5161 ttttactccc attaccaatt gttacgtttt tattttcctc attaaaaatt gtcacatctc
     5221 caccaggagt ttgcccagtt attactgcat cttttggttt ttccgataaa gtaatagtta
     5281 catctccagc agttgagctt gcctctacat catacttagg atcatgatcc ataatatata
     5341 catccccact agtcgttttt gctgttactt tccccataac ctttttaaag ttcacctcac
     5401 cagcttttgt agatccctct acgttcttag ctgttacata cttcaaatcc acttctcctg
     5461 ccagcgttcc aacatttacg cggtcacttt tcacatcact tatttctatt tgtccagctg
     5521 ctgaatttac tttgatctct tggtaagaac gttctggtac aaatatagtc aatgtagacg
     5581 tttgaaatcc gaaattgtaa aaggaaaaat caaacgaaac atcttttttt ctttccccaa
     5641 taacttttaa tgtgtcacca tcttcagtaa tacgaatttc ttgtttactt acatctcctg
     5701 tttgctttac ataaaaagag gcatccggag atttttggac gacaatatcc ccagccccta
     5761 acttaacttc tatatttttt aacgtttcgt ttttaattac gtcttcttta tcgccttttt
     5821 ctgcagtttg tactgctttt tcatatgttt gtgaaatccc tattactcca ataatgatac
     5881 aagcgatagc tactaacaat atttttttca tcatctactc cccttttatc attttgaagt
     5941 tccatatcac ataacgtacg cacaatcttt taaaccactt cgtactataa tacgctatca
     6001 tacataacaa caaacctact ccaacaaatg ttatactaac gaaaagatcc aaccatataa
     6061 aagtgcccat aaatactttt acgataacga aaaatggtgt cactacacta gcaatcccac
     6121 cgacccaaaa agaaaatata aaaccgataa ttgcaactaa agggcctaac acgataataa
     6181 aattaaataa actaagtcca atcgccgcca tgacagctct tgtaacattt gaagtcgatg
     6241 gatctttttt catttcatcg aaacgataca tcgcaagtaa ttcttttgca attactttag
     6301 gagaaccaag tccttttatt atttctaatt cagttttccc ttcttctatc ccaaactgaa
     6361 agtgctcttc ataatcaaat aaaatatctt tcctctcttc ttccggtaac tttctaagct
     6421 gattcgataa ttctctaagg aattgttctt tattcatccc aattcacccc ttcaataatt
     6481 tcttgtaccc ctttcgcaaa atcactccac tctgttacta acaaatgaag ttgcgtctct
     6541 ccttgctctg ttagctgata atatttacga ggcggtcctt ccgtggactc ttttaaatac
     6601 gtttgaaaat atccctcttt cgttaaacga cgtaataatg gatatactga accttccgat
     6661 attaaaaatt tatttgaaat ttgctgtaca agttcataac cgtaactatc tttacgtttc
     6721 acaagtgcca aaacacatag ctctaacaca ccttttttaa actgaacgtt caaatgtaat
     6781 cctcctttct tagtttagta ctgttcaata cacggtacta gatatatcca taatatacca
     6841 cttggtacta tttattgcaa ggtacttgtt tatatgtatt cactgacttt aaaaacgcgc
     6901 aaataaaatc ccgaataaga ttctgttgta gttcaccatt attcgagatt ttattcgatt
     6961 cgaactgcat tcatacaaag cttcattcaa tttttctcct ctaagaacca acttccattt
     7021 gtgaccctag taaatttcga aatacacctt ttctttcttt agctagttga ttaaattcac
     7081 ctttttgaac aatttcacct tttcctaaaa caataacttg atctgcattg cgtatagtag
     7141 ataagcgatg tgcaataaca acaatagtca ttgttccttt caatctttct attgctgatt
     7201 ggattttcac ttcattttca gtatctaacg cactcgtagc ctcatctaat actaaaatcg
     7261 acggttttct taaaatagct cgagccaata caagtcgctg acgttcacca cctgaaagcc
     7321 tcaccccacg atctccaatc agtgtatcaa gtccatttgg aagcattcgt acaaattcag
     7381 ttgctgccgc aaattctaat gcagcccaaa tttgttcttc atttacattc gagtcaatca
     7441 tcattaaatt ctctcttata ctagcattaa ataagaacgg atcttgtggt acatagctaa
     7501 tagcctgtct taatgataat aaattatcag ttgtaagagg ggtaccatct attaacactt
     7561 gacctttttc tggttggatt aaccccatta acacatcaat taatgtactt ttcccagcac
     7621 cagaatgtcc tacaattgcc gtcatactat ttattggaat ttgtacatta atgttttgaa
     7681 gtgcatataa agcttcttgc ttattataac gaaaagaaac atccttacac tcaatacctt
     7741 gttttataca aatcggcttt atatgttttt gcatgtatat atcctgcatt tcaaccgctt
     7801 cattacactc ctgttgcaat tcccataatg acttaaaggc aggaatgctt gcagccagtt
     7861 gttctaaatt tgactgaatc gtcatgaacc ttggccataa tcttgaaaaa atgagaatga
     7921 ttaatagtaa ctgggccgtt tgcgcttgaa acatatttac tgacaaaaat ataaaaaatg
     7981 caattaatat agccattgaa attttataaa acaattgaga gtttgacctt attctcatat
     8041 actccatttg ttcgctactc atcttttttg ttatagattg aaaccactcc aaacgtgatc
     8101 cttccaaagt attgctcttt atatctttaa ttccattgaa atgatctgtt ataccagaaa
     8161 gatagtcttt tgccaattct gaggtcttac cgcctagtat ccgtgctttt tttataaaaa
     8221 cacgagaagc taccaagaac aataagccaa agcccaaaac aaatacagtg acttggggag
     8281 ataataaaaa cgcaagccct acctgtatga atgtaaatag aatagtagct agcaattgta
     8341 aaattaaatt aatcccataa ctaacacgag ctaattctgt agttaatata ttaattagat
     8401 ttgttttcct tttttctaaa aaaaatctcc actttgcttt taaaatcata ctatatgttt
     8461 ctactcttaa ctttcgtaca aaaacttgtt gcgttctcgc atctcttaac gttatatttc
     8521 tttgcagtat attttgactt aacactatta atacatatat acctaaaata gaaattaaac
     8581 ttgttgtttt agggaaattc tgcaaaattc tatagacagg tgcaataaaa gaagtctcct
     8641 cacctgtatt aattattcca gtcatactta tcataggaag taataaaata atacctatcc
     8701 cttcaagtaa actgattaac accataccaa ataaattcac atacagtatt tttcctgctg
     8761 aagaatataa tttcttcata aaatatataa tataattcat cgatgttccc ccaataacaa
     8821 agcaaacttc ttcgtttttc tccaaataca tagaaacgga cgtaaaggaa aatataaaaa
     8881 atgaagaaat ttaggtaacg gcaatgtttt cgcatcaatt ggataaggat atagttggct
     8941 cataataaat aaagtttttt gctgattaga cattaacgaa aataaatgtc gactatgata
     9001 agcaagtact tcttcaggaa ccggatcggt atgcaagttt atcatttgct tgaaataaaa
     9061 caaggcttct tgcgctaact gttttgaatg ttttttagct actaattcca tcattctttc
     9121 atccatccgt acacctagca attgagatga caaaattaat gcttgtcctc ctaaatgtag
     9181 catttgatat gttttcatta gtttatgaat ctcactccaa ttaagatctt gattgacaat
     9241 tttttttata tctactaacc agcgtaatct ggaccaacca tgtctagcac catgagatac
     9301 aagaaataaa aataaatctt ccttccctag catatatacg gaattactcg taagggtgct
     9361 ttttctcttt cgcttccaaa gctcttcaaa cccaggttcc ttactaggtc cgggatttaa
     9421 ccgccaatga atctcaagct taatctgttt tgtcggatga aaaaatgtta tatgatgatg
     9481 cctccatttc caatcattta atatcgtttg aatataatcg tcttttacat acccctgctc
     9541 taaaagtagc atttctactt tttctaaatt ttgaattgga ataagaacgt ccaaatcaca
     9601 ggatgttcgt aaagaaatat ctccatataa atcctgagat agtacaggcc cttttagaaa
     9661 aatcatacga atctcatttt cattacatat tttatttatt cgttccattt cagcacttaa
     9721 ataaagcatt tgaaatgtat tttttttata ttttctattc aaggattgta caatatgtga
     9781 aggaattatt tcctcatcta cctgtttaag tttttgatat atcaccggat aaacgcgatg
     9841 atgcacaacc tgttcaagaa ataattccca atcaatatcc gaaaaccatt cattgctgta
     9901 agtttgcatt gtttcatcat cttttaactt caaaattgca aaaagaagtt tcaattcctt
     9961 agacatgaac tctacattaa gaccaaaatc tttctccatt ttactctcct ttaatagtgt
    10021 tctcacttag cctctttgca aatgatccaa cgacagtgaa tttctccatt ccttccgatc
    10081 cagtaacata aaaagaaccg ctacgtaacc atgcatgtgc aattagtgcc ccactattat
    10141 ccttagcagt tcctaaataa agagtactct caatgtcacg tttttcaagc attttcatcc
    10201 cagctatcgc tttaacgaga cattgacttt cccaaaaagt ataacgactc ataatagaaa
    10261 ttgcttcgga cacctccttt agtgtatcct cgtgttgctg aatttgagca actgatgttt
    10321 cgttcatgta atcccctaaa gaaggtgcaa ctttggaaaa ggtaatactt tttagtacac
    10381 gcgcccatcc taaaaaaata aaagcctcta aaaataaaag ctttgtttcc atgttcagca
    10441 ataaaaatac ccttaacctt ttcacaatat tcattaatcc cttcctaaca gttcttttga
    10501 aattacaaat ctatctatta ggctgatctt ttatttgaat taacccttct tgaaataaac
    10561 actctaaaaa ggaaagaact tgctcttcac aatcttttcg ttcaatatca tagttggata
    10621 ctagcatagt aacaagctga ttaattatta ttggcttttc aataagctcc catatttctc
    10681 cgcctatttc acctaagtta tagtactttc ctttctggac atttaacata actttttctc
    10741 catccatatc gcttactata ttacctttac tttggacaac gttatgtttt aatgatattt
    10801 ttctcgagtg gacaaccatc aaccatactc cctttctgta tagtatttaa aataacttcc
    10861 acaagctcat gagctgtaaa tcttgaagtc gggcgactta cttgaaacat ttccattcca
    10921 cttgcaatat gagttgaagt ttcaaaatgc cattccatta atcctaattt agaaatcaaa
    10981 gaactcctaa aagtatgacg gtataattta tacaagcgtg cgagcccttc tatacgggtc
    11041 gtctcaatat cttctccctg tgtttttact aattcaataa caccagcaag aggtaatgga
    11101 tctggaaaga attgagaatt cacaggaaca gcatacttcg tttctctttc aaacaaaggg
    11161 ctcaattgcg ctacttccat ccccaactca tttatgctct cttgccataa tttttgttgt
    11221 gcgtaagaag gatgaactat tgcagtattt ttattcaaaa gagacacagc aactacatca
    11281 tcacttaaaa gctgatatcc tttattcaaa aaagccgagg ctaacgttga ttttccagct
    11341 cctgaatcac caattaatgc gtatgctttt ccatctatgg caattacact cccatgcaat
    11401 gggagtgttt tacgttgcat taaaagcgcc cccatacaag ttcctagaat atataagcga
    11461 attttatctt cttctgcgtc tgccataggt gaaaaggtga ttttctcgcc ggcctgtata
    11521 gaaaatattg cagtttgagg aacatgaaac ataactacat tatcctccac tacaattgtt
    11581 ttttgctttt ccgcgctata tttacaccac tgttctgaca aatccgttat ttcaacttga
    11641 acatctgcca cctctttttt ttcattcagc tgagacaatt cgggtaatgg aatttcactt
    11701 actattttta acccgaaagc ttgatataac acattcccca tagaattaaa catcctatcc
    11761 cccacattgt caattttttg aaagctctta tataataaag ctatataaga gctttatttt
    11821 tccaatgact taactgtact taactggatc atctgggtca ggttgaacag catctggata
    11881 tttatgccca ggcccagcca tagtcatatt aatattaagt acttctaata caggttgttt
    11941 ccattccttt ttcatacgta tcacctcctt tcatattgct ttttttataa atcgatagaa
    12001 gattaagctg cgcattaaaa ctctaaaatc taaatcaaat gcatattctg gtcgaggttc
    12061 tttacaaatt tttgagatag ctttcttaat tacctctaca tttagaaatt cagagattac
    12121 tggatctaca ctaagttctt caacttcctc aataaattca ttccaaaaag gagccatacg
    12181 gtaaacacca tctgcacctt gaataccgcg aacccgttga ttcaatcgta ctttatcagg
    12241 aaggaagctc tttgtggctc tccggatcag ggctctatct aaaccatttt gaacatactg
    12301 ttcttctggg actgacaaac aaaattgaat caatcgtaag tcatttgtgg gatcacgatc
    12361 ccataaggca taacgtaatg ataatttcgt ttcatacgtg ccatttatgc tccaataata
    12421 tggtttttca aactgttctc ttttcatttc atatgcatta gaaatagcgg tgcctgttat
    12481 atcgatttct tgttctctaa gtctgtcaaa aacattcatt tcccttgcaa attccggatt
    12541 aattattacg gaatttgcat cttgttttcc tgaaaaaagt aactgatgca aaaatggaaa
    12601 tgcctttcgt cttaccactt caaaaacacg tgattttttt actcctatat ttctactata
    12661 taaatatagt tcacgataaa aacgaatcca atgtagcttt ttcaataaca ttgcctggta
    12721 atctaaaata ggtccccatg atactgtcca attacctctt tgaccactaa gaagtactcc
    12781 tattccctgc tgagatgctt tttcataaac tccactcaac caaaaagtat tctcaaaaaa
    12841 tttatatggc atctccattg tttccagcca atcatcaatt tccgatagtg ggcttctctc
    12901 tggcaattct aaataataat cctgtatatt ccctacatgc tctacagtcg attgaataaa
    12961 tggtctttcg tcagctatcc tacctttgtg tgtccaatct tcaaatcctt ctactggaac
    13021 ataactatac gtatgtaatt ttttattttc agtccgtaaa tcccttgcag caaaactaac
    13081 gacagaacct gagtctaacc ctccactcaa atgggctccg acttggtggt gtgttcgcaa
    13141 acgtgccttc actgcacttt tatacacctc tcgaaacgcc tcttcatact cttcatttga
    13201 ttttaaatta agcattttcc caacagttgg tgtatagtat cgtgaaaact ttacttcgcc
    13261 ttcctttacc aaaatagtgt gtgatggagg cacttgttct atatatttgt aaactgttaa
    13321 ctgtggatct accgattcga aatttacagg tatagctaaa aattcagcaa gccactgctc
    13381 attcaatttt ttctcaatat acggtagagt aaataacgga tttataatgg tacaaaaagc
    13441 aagtttttcc tcaccacgat aaaaataaag ggtccgactt cctgaaaaat ctctggcacc
    13501 aaaaagaatt tgttttttct catcccaaat cataaaggca aaatcgccta ccaaatattt
    13561 tggagccgct tccccccatt tctgataaga gagtaaaatc aactcactat ctgtcatgtt
    13621 ttttctatcc gaataatcca cttgtaattt ttcaaataac tcattacgat tatcaataat
    13681 agcatctgct gtaattgcca attgcttttc ataattataa aagggtaatt gttcaccaac
    13741 tgactctggt gtaatccatt gtgcatgaca tcctagaaaa gcattttctt tgtgccatat
    13801 ttgaatatcg tcagcagggt acttttgtaa ttcactcatt aatcgtgtgc catgttcaat
    13861 tgatataggt tcattattaa agtgaataat gccagttata gcgctcattt cttcctccta
    13921 atatatgaaa agttactaca attactattt acatattaaa ttcgttaaac ataccccttt
    13981 atcattcttt ttaaagaacg aaatttttta tttttattac tttctcaatt acatgcttta
    14041 atttactctc agacttatat attatagaca tttccaaatc tatactcctc ccttttttta
    14101 tgtaaattga aactgaacac tagaaaagat tcattataaa gaacaattag tggtacgtat
    14161 acaaatctaa ttcaaattcc attttcactt acttttcttt taattcttcc attctacagc
    14221 ttttaaaatg ctttgaatta ccctttgttg atcttctgct aacatattag atcctgaagg
    14281 caaacaaatt ccactttgaa ataggtactg cgacacatct tcatttttgc tatgcggata
    14341 atacttcatc tctttaaaga gcgactgcat atgaagtggc ttccacatag gacgcgcttc
    14401 aatgttctct tctgctaaaa ttctcaacaa tttcccaata gaaatcccag attccttttc
    14461 atcaattgtc aatgtagtaa gccaacgatt tgggcgagta tgttctaatt caggcataaa
    14521 ataaaaccct ggcatgtgag atagcgcttc gtaataccgt ttatatacaa atcttctagc
    14581 tctcacccta tcttctaaca cttctaattg tcctctacca ataccagcta aaatattact
    14641 cagtcgataa ttatacccta tctcactatg ctcataatgt ggagctggat cctttgcttg
    14701 tgtcgctaaa aatctcgctc tttccaatgc ttctacatca ttagaaacta gcattccacc
    14761 acctgaagtt gtaataattt tatttccatt aaatgaataa acaccaaatt tcccgaatgt
    14821 accgcttgct tttcctttat atgtagaacc aagagattct gctgcatcct caactatagg
    14881 aacattatat tgatgacaaa gtgagagaat ttcatctagt ttagcactct gaccatataa
    14941 atgaacaaga atgatagcct taggcaaatt ccccatttta ttagcttcat ataatgcatg
    15001 tgccaatgca ctcggtgaca tattccaggt ttccggttct gagtcaataa ataccggttc
    15061 cgctcccaaa taaacaatcg gatttgcact cgctacaaaa gtaaaactgg aacaaaatac
    15121 tgtatctccc ttttgaatac ctaataactg caaagctaaa tgaatagcag cagtacctga
    15181 actaactgca gtacctcctc taactcctac aaaagatgca agttctcgtt caaacccatc
    15241 gacatttggc ccaagaggag caacccagtt tgtatcaaat gcattttgga tatatttttg
    15301 ttcattccca ctcatgtgcg gagaggaaag gtaaattcgc tttttatgct gaactgtgtg
    15361 catgtaaaca catcctttat acatatttaa atactatgta tctaaaaaca tatgctttaa
    15421 tacagatata tcagataatc tccttaaatt atcaattgta taatgtgctt tataagcttg
    15481 ctcaactata agattatggt aaattccatc ttctctacta atatggatcg ttaaccattt
    15541 caatttcttg gccgttataa aatccttact taaattatct cctatataga cacattgctc
    15601 atgaggcacc tgtaattcct tacttatttt ttcataagga attttgcttg gtttccaaca
    15661 ttcttttcct agcttatcag tgagaattat tgaatgaacc ctctctttta actttaatgc
    15721 atttattttt cttttttgag caactaaata tccatctgaa ataagtccta ttttaacggt
    15781 attaaccaaa ttatttagta cccaatccgc ttcatccaac aattgaatat ctggtttatg
    15841 gaacctatac tgttctatca aattactaat caatttttca tcataatcaa tgtctaactt
    15901 tttaagtgtt ttattaaaca caaactttct ttcacctgaa ttgaataatc gaattgcgat
    15961 attataaaaa ccaatttttc catagttatc tttaatccaa tcatccactg ctttaaagcc
    16021 acttacgaca taatctttct ctttatataa agtatcatcc atatcaaaaa caattgcttg
    16081 aatcaccata ttaaatactc tccataaaaa ccgcttcgtc atatctaagc attgttaaat
    16141 tttctctcca attaaagtct ttatctataa agacttcccc ctgacacatt ttaattgtcc
    16201 aaagtggaaa attcgctcct gcttctattg atagtggtat tcctcctcca aaacgagggt
    16261 ttatttcaat aaacgtaatt tgcccatcct cctttttaaa acattgtaaa gtaatacatc
    16321 caataggatt aggaagagct tttattacag tttctgctgc ttggatgatg tcaaaatctt
    16381 tcttggtcac acctttactt acctcacctg ctcgtgtctc tatacgcaat ctaggaacga
    16441 tcgtttttat attcccatag aaatctacca tgacatctat agtgtattcc tctcctgaaa
    16501 cgtactcttg tatcatagcg ttaggtatat actccttaaa aaattccaat tccttttcat
    16561 tcattatttt tgttacacct ttactagaac tgccatcata aggtttaatt aatagtggaa
    16621 attgatattc ctttcttcca atctcttcat cactatacac tctaggagtt ggtatattat
    16681 tagagacaaa aaagttatat gtgtattttt tgttattagc aattttattt aattccatac
    16741 tagaaactag tacttttaca ccaatacctt caaaaacctc cgaattatta gccataagga
    16801 ttaattccgt atcaattaac ggaattatta gatttatcat ttcagatcta catattttta
    16861 acaattcatt tatatatcct tcttcattta cacgcggaac aataaaatgc ttatcgctaa
    16921 agtaaatggt aggagcagtt tcttttaagt ctgcagtaaa gattttccct ttgattccta
    16981 cttgttcaaa tacctcctta aattttttaa ttaaagtgac cctcctacca gagctagtaa
    17041 acaaaatgtt cattttattc ccccccatgc tcgattcccc ccttaaaaac tggcatagtc
    17101 acatgcttat tatttgttat cccttccgat tttaaaacct tactaaaagt tagatataaa
    17161 attttcaaat ctaaaagaaa actttgattc tcaacatacc atacatctaa tttaaatttc
    17221 tcatcccatg agatactatt tcttccatta atttgagccc atccagtgat tccgggttta
    17281 acattatgcc ttttagcttg ctcttgcgaa tataaaggca tatattccat taataatggt
    17341 cgagggccca ccaaacttaa ttgacctttt agcacattta ttaattgtgg taattcgtcc
    17401 aaactatatt tcctcaggaa ttttccaact ttagttaatc tagcgtaatc tggtaagagc
    17461 tccccgctat tatctcgctc attggtcatc gtacgaaatt tataaagata aaagggtttt
    17521 ccatgtaacc ctggacgttg ttgtctaaat aaaataggtg atcctatctg ggttttaact
    17581 ataattgcta ctaaaagaat aatgaaaaaa aagcaaatta ataataataa agaaataaag
    17641 atatccaaca gtctcttcat taccaatcat tcccccatct taaattatta caaatgcttt
    17701 tccaaagtga aataatatat aaagtattca ttacggattg aaatcaagta ttttcaagtt
    17761 gtaagtaaag aattgtattc aatttttctt tacttatcac ctgcaaacat tttctttagt
    17821 acacaacttt aaaacttaat aagtagaaga aagcaataca cataggtgca aaatatattt
    17881 tccaaatgct tatccaagag actattacac actgtaaaaa tctcaaaaag tatattcaca
    17941 aaaacaattt tataaacaac gcttaatagt ataaaaaaac atttattatt aaaacattct
    18001 cctactcact attttttagt atgcagttaa cgtaataaca aaaagctatt atgtttataa
    18061 aaaacataat agcttttctc ttttctatag tagtaactaa aagtcaataa gcatattatc
    18121 tattaggaat tagactattt cgaatgatta cattggacaa aacccttcca tgtaccatta
    18181 tataatactt tatagacata tttattaaga gccactctac ttcatttctc ataacttttt
    18241 gaaactattc aaatttaatt atcaccatgc attaatttga atcgattaca ccattaacaa
    18301 tatttccttg taaaaattcc ctccccgtaa ttgtaatttt cgcattattt aacacgttat
    18361 tttgaattac tacttgaggt gtactactcg ttgttctatt atctatccca acttgcatca
    18421 aatttgtgga atttataata ttattttgaa ttatcacttg cttaccagta aacgctgcat
    18481 ccaaaacaat aatagctctc tgaagagtat catttgtaat ttttatagta tttcccctta
    18541 acactatttc atcagataca ttttgaatat tcaagaccgt accatttggt ttttctatat
    18601 gacaatctat catcttgaat gctttgaggt tattcactgt aaataacgtg ttatttctat
    18661 ctatactaaa gaacttgcaa ccttttaatt ctaaagtgct tcctgtactt gctatcccca
    18721 aaaagattac atccttaaaa aagcaatttg tatacgtccc tgcaactagg gttgggggtt
    18781 taacattatt aaatataata ttttcaaata tccatcctga tttaactgtt cctactggta
    18841 cataatttaa atacaaaacg tcttgtcctt caaatataca atttgagata gtttgcggtt
    18901 catttttcag ctctaatgtc cactgataca atgatgacaa ggaattaagt ttatctgtat
    18961 cgtttataaa acgacaggaa tcaactttaa ccgcatatgg aaagggagta tcaataatca
    19021 ttttactatt agaaaaaaca ttatccgata tattaataga tcgatttaca tatacacctt
    19081 gaacatttac ttgcgctccg tatacataat tattggaaaa aataacatct cctaacaaag
    19141 agaccttggt taatcggata ttattccctg ttacgattac cctatctgca ccaccattaa
    19201 tagacaaccc aacataagca tttgtaatgg tattcataat agaattattc gatatataga
    19261 taaatttccc atttacaaca ataatattat aatttttatt atcatgaaaa ttatttcttt
    19321 caatatatat atattgattt aaatcatatc catcttcaat atctagtcca ctttgtggat
    19381 tggtacctgc gatgtgatga atatcattat ctcgaatata tacattcttt gccccacata
    19441 cactaatccc ttgtctacgg caatcatgta aattacattt ttctatataa gtatattgcg
    19501 aaaaagaagg tacccgaaca tttattaaac aatttgcagg agcaggaaca ttcccttgat
    19561 gcaaaacaac tttagcataa cttgctccat tagggacttc tacttcatca aagaattgta
    19621 cattggtttt tgaagataaa aatatattgt ttgaagtgta aaaaatgaca tcataataat
    19681 cgcattttat ttcagaaccc aatgctccaa atccatttcc atataatcca aagtaaccat
    19741 actttacaat agccacttgg ttcatatcaa tttgcaaatt ggaacgtatc tttgttgtac
    19801 tacttaccaa tgaaccatct gttgtattaa ttgctccctg ttcaaatgaa cttgctaatg
    19861 ctgtaggaaa aggtgaaatc tgcccaaaag tactgttcag tgtaatggca tctcctgtac
    19921 aatctaaaat atctagatta tctaaagata caaaccgtgt attgttcccc ccatcagcag
    19981 gtggagtaaa actccccaat tcaatgccgt accctgcctc atgtgttccc ccactagagt
    20041 aatcatgtgt atatctatct ccttggataa taccatttgt tatcctagaa tatacttgat
    20101 ttctacgaaa acttataatt gcatatccag tcaaattatt tgtttccatt tttaatgtcg
    20161 aaccgtttag attcaaagtt aaatgacttg gtggttcgat gggtgtttgt ttatcaatca
    20221 aatatattcc tctaggtaac acaacttccg tataaccttg ttgagccgcc catactagag
    20281 cattattaat accttgggaa gtattgatag catcagtccc atcattctta acatcccatc
    20341 tatctaattc taatacatat atacttgtag aatttgtagc ttcaaatgat tttggaatcc
    20401 attttaattg cgtactatca taagttaaaa tgtcattatg aaaaggaggc ttggacacca
    20461 aatctaaatc attcaaattc ccaagattat cccttcttct ccacatcaat tcacacctct
    20521 tttttgcttt tctacatctt atgtttcata aggtaaattg attggatata agagaagtaa
    20581 tatcactttt tattgttttt agagatccat tatttagaaa gtctttttac aattcctttc
    20641 taaaaaggtc tattcttact agctgttttc atttcaatac tcttttattt ataactattt
    20701 aaacttacaa tattattaaa aagacactat actctttata cctaacgccc ctattattac
    20761 ctactattat tttcagaaca acctaaaatc tcttccgtaa ataaagcatt ttttactgtg
    20821 atatggaaaa aataaaatta taatttatgg ataattatca ttcggaaatt tccgagttaa
    20881 tattttcggc tttatttttt ttattaatat ccgggttaaa ttaaggaaat atctatactc
    20941 ttccgcctct ttaaatacca tccaaaagat cagattcgga ataatcagac acaaaatact
    21001 acgtattgcc atgtccataa atatatttat gtgaaaaata taaccaataa aatccgttgc
    21061 tataatagaa aataatacaa gcactacaaa caaaatatat ctaataaaat atacttttac
    21121 agattttttg aaaatcattt taaatataat ataagggtca taccaaaagt aagtacataa
    21181 tctactaata acagtcccta aaagcactcc tgctattcct atttgtttcg caagaattat
    21241 agaaactaca atatttataa ttgccgctat aataggtctg tacttccctt ttttaaataa
    21301 acctgtggtt tctctaaaca ttgtagttgt attttgcatt cctgaagtga aaaaattaag
    21361 taatatgaaa aataatacat acttattaaa cacatattcc tcacctaacc agattgttat
    21421 aaaaggattt atcaaattcc aaaggcaaac tgtacaaaat ccatatatcc aaaaatttaa
    21481 aaagttaatt acacgaaaaa taaaatattt tttttctgca ttttctttca cattcaaatt
    21541 tccaacacta gcagtgatag aataaaacaa atagcctaat aaggtattta atgtatttaa
    21601 gatcaacaaa taatttgaat aaaccccaac ccaaataacc ccaataaatt tcgatataac
    21661 tatattatct gttgcattaa ttactacacc actaatttta tatagcaaca aggaatacaa
    21721 attctcaaaa aactcacgtc tgtctgtctt agataatttc gcattatttt tttcttttaa
    21781 aaatggatat aatttatttg ccttatttgc aatatataca ttctcaatta cttttaatgt
    21841 taattgagct accagtacta caatgtaatc actaataatt attaataaaa caatttgtaa
    21901 tgcattagat ataatcatga acaaagtgtg tattttagaa ataatatagt tactttgatc
    21961 agcaataata atagattgct tatatacaaa ataatatgat gacactgaac ttattaaaaa
    22021 taaaagatag ataatgttaa tgtctttaat cgtagtatct gcattcacta aatgatcaag
    22081 aaatggcaat agagataacc ctattaataa aactactact cctattaatc tgtatgcttt
    22141 ttgatataaa ttcattaatg cctgtatttt ataaatatcc ttctcagcta atggcctata
    22201 taaactatat attatagctg tatcaaatcc taaattagct agggaaaaca tcaatagtat
    22261 acttgtaaaa agcccatcca cacctaaata ttctataccg agtgtaaaaa taaaaacagt
    22321 tcgaacaaca aatcccatac caatactaat tatttgtcca gacaaaccga ataaaatatt
    22381 ttttaaggag ttttgtattc tcatcgtaat accttctata ttttaattgt tttgcgtacc
    22441 cactctacta aatttggcac taatcttcct ctttaaaatc gggaaaaact ttgttatcct
    22501 gtattttaat aacgcttttc ttcgttgcgc cttgaaatca taatctagaa accaatttag
    22561 taaattttta tctgtaatac aatcacaatt ttgtttcatt cgcacttgga ttaaattcca
    22621 atacatttca atgaatgtat tagtaaaaaa cttcacctca ttatttaaag aagttttaac
    22681 gtactgataa agattacttg gaatattaaa accatattca cgagggtctc tcccttcagc
    22741 aataattccc ataattgttc ttaaacattt ttcacattta caacaatttt cttcattttt
    22801 aaaacatacc ctgatattaa caggttcttt actagctgaa taatggtcca caacaacttt
    22861 aatcttttct tgtctagtaa gttcgtatcc atcatgaaag actgttccag atgcatattt
    22921 tatttcatta tccacagttg gatcagatgc acaagtaact tgatataatg gggaattaga
    22981 acttgcaata tatatacatt ttatcttaag tttaaaagct attggaatag ctgcagatat
    23041 tattgctaga ccatgatgca atccatgcca ccatgtatcg tttaactttt ctctaaaatc
    23101 atggtcaatg ttccctgcaa ttataaaggt cccatagtta gattgtataa aaatattttc
    23161 tagtccatta tcctttacaa aatttacggc attttcttta tctgcctccc atactttact
    23221 accctctatt tcattttcat gccatccata ttcagtaact agcaaggggt gtttatcttt
    23281 tattcttata aatgtagtaa gtgcatccaa tccaccacta aataaagatg ccgcttcaag
    23341 ttccggaatg taaacattat caataatttc tcctacaacc atggatcctt taaatttcac
    23401 accaggaaac atgttttgat atgcattttt tatattattt aaacaaatat aaaaagattt
    23461 atctaattca tttattacta ttgttgaatt agtaatccat acaagaggaa ctacattcgc
    23521 aacaaacgga atagccaaaa tacttaaaga gatgtcagat acatcatagt tatattcaag
    23581 aaacatatga ttacttgtct taaaatactt ttgtaaatcc cccttaatac taaaataata
    23641 atcaactcta ttatttctta cgacaatttt atcgagaatt atactattca tttgagcctc
    23701 ctaataaatc gccattttta tcacaataca tttgttaatt agagaactgt tttaaccatt
    23761 gatttactac atcaaaattc acttgaacat ccgtgataaa ctctgcattt atcatttttt
    23821 cgagattatt taaatcttct ctatcaatca gatttaaatt atacgtttcc cccatcccct
    23881 tagctctttc atctatagag ataattat
//
