LOCUS       NZ_KB976671            10267 bp    DNA     linear   CON 01-APR-2024
DEFINITION  Bacillus toyonensis strain HuA2-3 acrHg-supercont1.9, whole genome
            shotgun sequence.
ACCESSION   NZ_KB976671
VERSION     NZ_KB976671.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus toyonensis
  ORGANISM  Bacillus toyonensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 4008403)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C.,
            Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T.,
            Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     The Genome Sequence of Bacillus cereus HuA2-3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4008403)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C.,
            Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T.,
            Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2012) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_000399405.1
            Current Name                      :: Bacillus toyonensis
            Previous Name                     :: Bacillus cereus HuA2-3
            Date Updated                      :: 2022-03-10
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_000496285.1
            A1 Name                           :: Bacillus toyonensis
            A1 ANI                            :: 99.4201%
            A1 Query Coverage                 :: 83.75%
            A1 Subject Coverage               :: 94.68%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_006094295.1
            A2 Name                           :: Bacillus cereus
            A2 ANI                            :: 91.7421%
            A2 Query Coverage                 :: 77.41%
            A2 Subject Coverage               :: 80.96%
            ##Taxonomic-Update-Statistics-END##
            ##Genome-Assembly-Data-START##
            Assembly Method                   :: allpaths v. R41865
            Assembly Name                     :: Baci_cere_HuA2-3_V1
            Genome Coverage                   :: 108.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000399405.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 01:17:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,841
            CDSs (total)                      :: 5,738
            Genes (coding)                    :: 5,542
            CDSs (with protein)               :: 5,542
            Genes (RNA)                       :: 103
            rRNAs                             :: 6, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 3, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 81 of 196
            Pseudo Genes (incomplete)         :: 115 of 196
            Pseudo Genes (internal stop)      :: 62 of 196
            Pseudo Genes (multiple problems)  :: 52 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:43:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1838671
            Orig. end                         :: 1848938
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB976671.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            The organism was changed from 'Bacillus cereus HuA2-3' to 'Bacillus
            toyonensis' in March 2022.
            Please be aware that the annotation is done automatically with
            little or no manual curation.
FEATURES             Location/Qualifiers
     region          1..10267
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10267
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10267
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5267](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5267
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            297..1211
                     /locus_tag="IG5_RS17355"
                     /old_locus_tag="IG5_01858"
     CDS             297..1211
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017673335.1"
                     /locus_tag="IG5_RS17355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01858"
                     /product="DMT family transporter"
                     /protein_id="WP_000932797.1"
                     /transl_table=11
                     /translation="MLNKKWDLQIICAHAFTILIWGTAFPTIRMGLESYTPEHLTLLRL
                     LIASFILLVFSFIYKLRLPDLKDIPAIFIFGALGFTIYHIALNYGEKTVNAGSASLIIS
                     VTPIVTAILASVFLNEKMKLNGWIGGVISFIGIALISFSQGGSIQFNSGGLFILLAAVS
                     ESLFFVFQTSYLKKYGFLPFTIYTILSSTVCMLIFLPGIYTETLAAPLEVNVSVIYLGL
                     FPTVLPYLALAYIISRAGASEATSSLYLTPVTACFIAWVWLGEVPTFISVIGGGITILG
                     ILIAHIRVVRKEKHRNLVNNKSI"
     gene            1350..2711
                     /locus_tag="IG5_RS17360"
                     /old_locus_tag="IG5_01859"
     CDS             1350..2711
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080598.1"
                     /locus_tag="IG5_RS17360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01859"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_001080598.1"
                     /transl_table=11
                     /translation="MNRKRNIFICLSIIGIGLASVSNSSFAANVTDSSVQHSIPVMNQQ
                     VAVATEMKPFPQQVNYAGVIKPNHVTQESLNASVISYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMVITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADNKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGSVNYLKEAQNMITKGIKAS
                     NVTNNNRLNLGDWDSKSSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFNN
                     KYSPNTGLISDFVVKNPPQPAPKDFLDESKYTNSYYYNASRVPLRIVMDYAMYGEKRSK
                     VISDKVSSWIQNKTNGNPSKIVDGYQLNGSNIGSYSTAVFVSPFIAASITSNNNQKWVN
                     SGWDWMKNKRESYFSDSYNLLTMLFITGNWWKPVPDDKETQNLINDEMYEGYDK"
     gene            2875..3798
                     /locus_tag="IG5_RS17365"
                     /old_locus_tag="IG5_01860"
     CDS             2875..3798
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="IG5_RS17365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01860"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_000416768.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATKPLTEEISTYYPEIHGLEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGKDLTIVDVGRSTSLAIAFNLWNDLILNVKGIYFMGGVFLEVGNVTPLA
                     EANVYGDPIASKIVFQQAKNLFIFPLNVTNKAVLTPNVFNYIQANSKNPFHTIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVVAISGLVNPSFFEFAHRTVNVDIYGDTKGQTFADFRPS
                     SISKGARIALEIDEQKFIQDFIKIML"
     gene            3875..4333
                     /locus_tag="IG5_RS17370"
                     /old_locus_tag="IG5_01861"
     CDS             3875..4333
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="IG5_RS17370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01861"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_001102693.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEEALQLQVKEDQRKFVPAVAVSLAKVYIKPDG
                     DNVEYIPFAIYVSNLMVGFIMHAVVRETSNMYWINGFIIDQKQQGNGYGKSALQESIYL
                     IKNKFKACKEIRLTVHKENISAKKLYEHYGFQPLGQDYDGEQVYRLFV"
     gene            4414..4803
                     /locus_tag="IG5_RS17375"
                     /pseudo=""
     CDS             4414..4803
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="IG5_RS17375"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="NUDIX domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MIAEYIGHHYFLPGGHVEIGESAENALIRELREGA"
     gene            5001..5267
                     /locus_tag="IG5_RS17380"
                     /old_locus_tag="IG5_01862"
     CDS             5001..5267
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071381.1"
                     /locus_tag="IG5_RS17380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01862"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001071376.1"
                     /sec_met_domain="TIGR03601 (E-value: 8.4e-48, bitscore:
                     158.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQSLNLNDYQSGNVVYWDQQQNQYPYYYIQDDARRCGG
                     CGGCGGRCGGCGGRCGGCAGRCGGCVGCAGCFSCFNCWNWWII"
     gene            5366..6382
                     /locus_tag="IG5_RS17385"
                     /old_locus_tag="IG5_01863"
     CDS             5366..6382
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="IG5_RS17385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01863"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_000288922.1"
                     /transl_table=11
                     /translation="MYTYEKLISWVENIKEKNHSSATALCIIKDNKIVLEHYSGYHSNT
                     STSKKVTASSQFNVASARKSYLGFMVAYALYDGKINSIDDEAIKYFKDFDPALLDKTTI
                     RHLVTHSHGLGETDDGTIFREFEAGQDWAYRDINVRMMTHLIYRLYNKSFPELLKERVF
                     TPANFQETGWKIQQNENLVNVVNNPNEDAISEVGTVDDGTEKNLFVSAREFAYWGNLHL
                     NQGMINGKQVVPKEVIKIATSLQSPAYKNNELPQNGLFWFVQNEPKQLSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFTSCNRA"
     gene            6414..6557
                     /locus_tag="IG5_RS31205"
                     /old_locus_tag="IG5_01864"
     CDS             6414..6557
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="IG5_RS31205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01864"
                     /product="hypothetical protein"
                     /protein_id="WP_000653682.1"
                     /transl_table=11
                     /translation="MKDKVSKDKKQNIKDVNTEQNAIFSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            6838..8046
                     /locus_tag="IG5_RS17395"
                     /old_locus_tag="IG5_01865"
     CDS             6838..8046
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="IG5_RS17395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01865"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_000815141.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQRKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDSLGVS
                     QAFLYWGIIVLLLVLIGSLFLREAIVSNTVTETLHNDYTPREMMRTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAVFNTVGRIILGTLSDKIGRMKIVSA
                     TFIIIGVSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFQPTFITIGVLCAISFIISILIRPPNVEKKKEIKQLHR
                     KIA"
     gene            8180..8596
                     /locus_tag="IG5_RS17400"
                     /old_locus_tag="IG5_01866"
     CDS             8180..8596
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016104977.1"
                     /locus_tag="IG5_RS17400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01866"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_000706728.1"
                     /transl_table=11
                     /translation="MKIVKQWVQEDSDYIREKVIEYNQKHLSNEEKTPSEKVSFIVKNE
                     KEEIVGGITAITFWHHVHVDFLWVSEECRHEGYGSKLIKLIEEFAIEKGCRLINLDTFS
                     FQAPDFYKKHGFIVIGVSEDHPKGHNHYYLEKRL"
     gene            8751..9194
                     /locus_tag="IG5_RS17405"
                     /old_locus_tag="IG5_01867"
     CDS             8751..9194
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757631.1"
                     /locus_tag="IG5_RS17405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01867"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_000757645.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDKTNEKILMVKNKGKNGSYYTLPGGSVKFGETL
                     EEAVIREVKEETGLHITVKGICSISEAFFEERGHHAIFFNFLGEIIGGETYISRPKEIE
                     EITWMELHIAAPYLRIPEQLLDFLQRKETVPYFFNGTIVHQSL"
     misc_feature    9241..9256
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.2"
                     /tool="antismash"
     misc_feature    complement(9377..9390)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 25.47"
                     /tool="antismash"
ORIGIN
        1 acagtactcc gatttacatg aaataacttt gctaactgcc gctggctagg aatcttactt
       61 ccgattggcc attctccgtt ttcaattttt tctttcatat agtctattat ctgccgatat
      121 ttgggcacct ttttatcgat tgtcatataa aaaccccctc gtttatttaa aaatgaaaag
      181 tggttggttt tttcgataaa taactggttg aagacattgt atcaactatt aaatatgatg
      241 aaaagaaata attggcaatt tgaaaatggt tggtttattg gagaggaaga gaaaacatgc
      301 ttaataaaaa atgggattta caaatcatat gtgctcacgc ttttacgatt ctaatatggg
      361 gaactgcatt tccgacaatt cgtatggggc ttgaatcata cacacctgaa catcttactt
      421 tactacgctt gttaatcgct tcattcatac ttcttgtgtt ttcctttata tacaaactgc
      481 gattacctga tttgaaagac attccagcga tttttatatt cggtgcttta gggtttacta
      541 tttatcacat tgcattaaac tacggcgaaa aaacagtaaa tgctggatct gcaagtttaa
      601 taatttcagt cactccaata gtgacggcaa ttcttgcttc tgtttttttg aatgaaaaaa
      661 tgaaattaaa tggctggatc ggtggtgtaa ttagttttat tggaattgct ctcatatcat
      721 ttagtcaagg aggttctatt caatttaata gtggaggatt atttatatta ttagcagccg
      781 tttcagaaag cctatttttc gttttccaaa cttcttactt gaaaaagtac ggctttttgc
      841 catttaccat atatacaatt ttatctagca ctgtatgtat gcttattttc ttaccgggaa
      901 tttatacaga aacactagca gctcctcttg aagtgaatgt gagtgttata tatttaggct
      961 tatttccaac agtactcccg tatcttgcct tagcgtatat tatatctcgt gctggtgctt
     1021 ccgaggcaac aagttctcta tatttaacgc cagtcactgc gtgctttata gcttgggtat
     1081 ggttaggtga agtaccgact tttatttctg taattggagg tgggattact atacttggga
     1141 tattgattgc tcatatacga gtagtaagaa aagaaaaaca taggaatcta gtaaacaata
     1201 aatcgatata acttaattag ataatgattt tatgatactt tttgatatag tttctttctt
     1261 atgttgataa atataatatt ttgtatttca gttaaatatt gtatgttaca ttttatgtag
     1321 taaacatttt aaaaggagat gataacataa tgaatagaaa aagaaatatt tttatatgcc
     1381 tttctattat aggtatcgga ttagctagtg tttctaattc tagttttgca gcaaatgtaa
     1441 cggatagttc agtacaacat tctatccctg tcatgaatca acaagtagct gttgcaacag
     1501 aaatgaaacc atttcctcag caagttaatt atgcaggtgt tataaaaccg aatcatgtta
     1561 cacaggaaag cttaaatgct tctgtaataa gttactacga taattggaaa aagaaatatt
     1621 tgaaaaatga tttatcttct ttacctggtg gttattatgt aaaaggagag attacgggtg
     1681 atgccgatgg gtttaagcca cttggaactt cagagggaca agggtatggg atggtaatta
     1741 cagtattaat ggctggttat gattcgaatg cccaaaaaat ctatgacggt ttatttaaaa
     1801 cagcaagaac ttttaaaagc tctcaaaatc ctaatttaat ggggtgggtt gtagcagata
     1861 ataaaaaagc acaaggacat tttgattctg ctactgatgg agatttagat attgcatatt
     1921 cacttctcct tgctcataag cagtggggat caaatggatc agttaattat ttgaaagaag
     1981 cacaaaacat gattacaaaa ggtattaaag ctagtaatgt tacgaataat aaccgactaa
     2041 atttaggaga ttgggattct aaaagttcac ttgatacgag gccatctgat tggatgatgt
     2101 cacaccttag agcattttat gaatttacag gtgataaaac ttggcttact gttattaata
     2161 atttgtacga tgtatatacg caatttaata ataagtactc tccaaataca gggcttattt
     2221 cagattttgt tgtaaaaaat ccaccacaac ccgcacctaa agatttctta gatgagtcaa
     2281 aatatacaaa ttcatattat tacaatgcta gtcgggtacc attaagaatt gtaatggatt
     2341 atgcgatgta cggcgagaaa agaagtaaag tcatctctga taaagtctct tcatggattc
     2401 aaaataagac gaatggaaat ccttctaaaa ttgtggatgg ctatcaatta aacggatcaa
     2461 atattggtag ttattcaact gctgtattcg tttcaccatt tattgctgca agtataacga
     2521 gtaataataa tcaaaagtgg gtaaatagtg gttgggattg gatgaagaat aagcgagaaa
     2581 gctattttag tgatagttat aatttattaa cgatgctatt tattacggga aattggtgga
     2641 agcctgtacc tgatgataaa gaaacacaaa atctaataaa tgatgaaatg tatgaaggat
     2701 atgataaata atagaatttg ataatggtta gctgggagaa ggaattagca tgcgctagtt
     2761 cctttttaat atcccgtaac acattatcca ttctaaaatt tgcatagata tacttgcatt
     2821 tttcatatac ataactattg tttgctttat ttttttatgg aaggagagta tttcttggaa
     2881 aaagtattat tttttggaga tccaggaatt gatgactcat ttgccattat gtatggttta
     2941 ctgcatcccg aaattgaaat tgtcggtatt gtaactggat atggaaatgt tgagcatata
     3001 catgctgcac ataatgcggc gtatatttta cagttagcaa ataggcaaga cattcctgtc
     3061 attagcggcg caacaaaacc acttactgaa gaaatttcaa cgtactatcc tgaaattcat
     3121 ggattagaag gattagggcc catacatgtt ccagaaagcg tattatctat tccgatatat
     3181 aattttggta gtattgctgc aattcttgta aagtatggaa aagatttaac aattgttgat
     3241 gtaggaagat ctacttcatt agcgattgct tttaatttat ggaatgactt aatactaaat
     3301 gtaaaaggaa tatatttcat gggtggtgtc tttttagaag taggaaacgt tactccatta
     3361 gcagaggcaa atgtgtatgg agatcctata gcaagtaaaa ttgtttttca gcaagcaaag
     3421 aatttattca tattcccttt aaatgtaaca aataaagctg tgttaactcc aaatgtattt
     3481 aattatatcc aagctaattc aaagaatcca tttcatacga taatgaaacc gatgtatgat
     3541 tactatttat ctgcatacca aaaacttaat ccatctatcg aagggccttt gctacatgat
     3601 gtggttgcta taagtggatt agttaaccca agtttttttg aatttgcgca tcgtacagtg
     3661 aatgtagata tatacggaga tacgaaagga caaacatttg ctgattttcg ccctagctca
     3721 atatctaaag gggcccgtat agcattagaa atagatgaac aaaaatttat tcaagatttt
     3781 ataaaaatca tgttataagc tgttgtcgaa aaagggaatt atttcactac gttgaatata
     3841 aaaatatatc ctctttataa aggagcttac aatattgaat gtccagttaa aggttgttac
     3901 gagagagaat tgggaagagg cattacagtt acaagttaaa gaagatcaaa ggaaatttgt
     3961 ccccgccgta gcagtttcac ttgctaaagt atatataaag ccagatggtg acaatgtaga
     4021 gtacatacca tttgctatat acgttagtaa tcttatggtt ggttttatta tgcatgcggt
     4081 tgtaagagag acatcaaata tgtattggat aaacgggttt attattgatc aaaagcagca
     4141 aggcaatggt tatggaaaat cagcattaca agaaagcatt tatttaataa aaaataaatt
     4201 taaggcgtgt aaagaaatta gattaacagt acataaagag aatatctctg caaagaaact
     4261 atatgaacat tacggttttc aaccattagg gcaagattat gacggtgagc aagtatatcg
     4321 tttatttgtt taacttttat taagtattag cgtgaaatga aaataactac tttaatcaag
     4381 gtaatagtga atgactagaa agatgaaaaa ttattgattg ctgaatatat tgggcatcac
     4441 tattttttac ctggcggcca tgttgaaatt ggggaatcag cagagaatgc attaataaga
     4501 gaactaagag aaggagcttg aagtaaactg cagtataaaa cgatttttag gagttataga
     4561 aaaccaatgg caagataaag aaatacttca tcatgaaatc aaccacattt tgaagtatct
     4621 tcaaaagagt tacaaattga tttaccacca aattctagag aatctcattt agcatttcat
     4681 tggatagatt atagtaaaga atctttaaaa acttataaaa tcatgccaat gccctccgtt
     4741 aaagaattac tagaaagaaa attaaatgat gaactactaa attgttggat aagtaatttt
     4801 taaatcactc tatgtatagt aaatacgtcc ttcgaaagga tataaagaaa ataggctaaa
     4861 aaacaaggta catcacctag tgtaagttca tattttgttg atgtacgtat ttagtctgta
     4921 gctttgtttc tatttggttt acaaaaatat ataaactttt tacatttggg cggaattact
     4981 tataaatgga ggggttcgta atgaatcagt ttcaacaaga actacaatca ttaaacctta
     5041 atgattatca atctggtaat gtagtttact gggatcaaca acaaaatcaa tatccatact
     5101 attatattca agacgatgca cgtcgttgcg gtggatgcgg aggttgtggt ggacgctgtg
     5161 gtggatgtgg cggcagatgc ggtggttgtg caggacgttg cggcggatgt gtaggttgcg
     5221 caggatgttt cagttgcttt aattgctgga actggtggat catttaagta tagaaaatca
     5281 ataatatttc acatgaaaga aaggtgttta tagtaatact tttctttcat gttgtgattt
     5341 tttatgagta ttaaggggga tttatttgta tacatacgaa aagttgattt cttgggtaga
     5401 gaatataaaa gaaaaaaatc atagttctgc tacagcacta tgtattataa aggataacaa
     5461 aattgtacta gagcattata gtggatatca ttcaaataca tctactagta aaaaagtaac
     5521 agcatcttca cagttcaacg tcgcttctgc gagaaaaagt tatttaggat tcatggtagc
     5581 gtatgcgctt tatgatggga aaataaactc tattgatgat gaagcgataa agtattttaa
     5641 agacttcgat cctgcattac ttgataaaac aacgattagg catttagtaa ctcattcaca
     5701 cggtctagga gaaacggatg acgggacaat tttccgtgaa tttgaagctg gacaagattg
     5761 ggcatataga gatattaatg taagaatgat gacgcatctc atttatcggc tttataataa
     5821 aagttttcct gaattgttaa aggagcgtgt atttactcct gctaatttcc aagaaacagg
     5881 atggaaaata cagcaaaatg aaaatttagt taacgttgtt aataatccaa atgaagacgc
     5941 gatcagtgag gtcggtacag tagatgacgg taccgaaaaa aatctgtttg tctcagctag
     6001 agagtttgct tattggggca accttcattt aaatcaaggc atgataaatg gtaaacaagt
     6061 tgttccaaaa gaagttataa aaattgctac tagcttgcag agtccagctt ataaaaataa
     6121 tgaactacca caaaacgggt tgttttggtt cgttcaaaat gaaccaaaac aattaagtga
     6181 acttggtgaa cgtgttccaa aaggttcgta tcaaatatta ggaattactg gaccaaccat
     6241 tttagtaata cctgagtata atgtagttgt tgcgaaaatg tacaataaaa gatataacta
     6301 cggcggtgat aattacttat attatttacg tgaatttagc aatttagtcg ccgatacatt
     6361 tactagctgt aatagggcat aattactcct ataacaaaaa ggagtgaaag aatttgaaag
     6421 ataaagtgag caaagataaa aagcaaaata taaaagatgt aaatacagag caaaatgcga
     6481 tttttagcga tccgaaagat gcagctaaca tgcaaactgt accgcagccg aaagattttg
     6541 atgaaatcga atattaattt ctcatccgac tatatgacga ataaaaaata cgtcttaagt
     6601 cccatcacat gtaagaaaat atttgataac atatatacat aagcacttga aaatggctta
     6661 tcttaacaaa gctttcttta aatgtatctg atataacctg cgaaaagaca cttcatgatg
     6721 aagtgtcttt ttgtgtacat aaacttaatt tccatttttc gtacagtatc ttgcaattta
     6781 tgatggatat actaactgtg aaaattcatg tcaagataaa agaaggggac agttagtatg
     6841 aaacaaactt ctataaatcc gctattaatt gttctaggta caatcattgt tcaaattggc
     6901 cttggaacaa tttatacatg gagtttattt aatcaacccc ttgtaagtaa gttcggatgg
     6961 aatctcaatt cagttgcgat aactttctcc ataacaagct tttctttatc attttcaacc
     7021 ttgtttgcag caaagttgca gcgaaaatta ggacttcgta aacttatcgc tactgcagga
     7081 attgttctag gactcggttt aatacttagt tcacaagttt cttccttacc attactatat
     7141 ttattagctg gtgttgttgt tgggtatgcg gatggaacag cttatattac gtcactatct
     7201 aatttaatta aatggtttcc aaatcgaaaa ggacttattt ctggaatatc tgtttcagca
     7261 tatggaatgg gcagtttaat ttttagatat ataaacggaa atcttatcga tagcctaggt
     7321 gtatcacaag catttttata ttggggcatt atcgtcttac ttttagtatt aatcggatcg
     7381 ctcttcttac gtgaagcgat tgtaagtaat acggtaacag aaacattaca caatgattat
     7441 acgccacgtg aaatgatgag aacgaaacaa gtatatctat tgtttttcat gttattcaca
     7501 tcgtgtatgg gtggtctgta cttaatcggt atggtaaaag atatcggcgt acaactcgtt
     7561 ggacttagcg cagcaaccgc cgctaacgcc gtcgcaatga ttgcagtctt taatacagta
     7621 ggtagaatca ttcttggaac gttatcagat aaaattggta gaatgaaaat tgtctctgca
     7681 acatttatta ttattggagt atccgtcttt actcttagtt tcattccatt aaattacggg
     7741 atttattttg cttgtgtagc aagtgtcgcc ttttgcttcg gcggtaatat tacaatattc
     7801 ccagccattg tcggagattt tttcggttta aaaaatcata gtacaaatta cgggattgta
     7861 tatcaaggtt tcggatttgg tgcacttgca ggttcattca ttggagcatt acttggtgga
     7921 tttcaaccaa cctttattac aataggtgtt ttatgtgcga tttctttcat tatttctata
     7981 ttaattcgtc cgccaaatgt cgagaagaaa aaggaaatca agcagttaca tcgaaaaata
     8041 gcttagtttt aaaaagaggc cttatcgtag ggctcttttt cattacaacg ttaactatgc
     8101 tcctttcatt gattagtaaa gacaggacaa tacaattatg aaatggaata gataaaataa
     8161 aaggagtgtg atatgtaaaa tgaagatagt aaagcaatgg gttcaagaag atagtgatta
     8221 catacgagag aaagtaattg aatataatca aaaacatctt tcaaatgaag agaaaacacc
     8281 ttcggaaaag gttagtttta tagtgaaaaa tgaaaaagag gaaatagtag gtgggataac
     8341 agctataaca ttttggcacc atgtacatgt tgatttccta tgggtttctg aagaatgcag
     8401 acacgaagga tacggaagta aattaataaa acttatcgaa gaatttgcaa ttgaaaaagg
     8461 atgtaggcta atcaacttag atacgtttag ctttcaagct ccggattttt ataaaaaaca
     8521 tggatttata gtcattggag tgagtgaaga tcatccaaaa ggacataatc attattattt
     8581 agagaaaagg ttataaagtg tatagaatta attaatctca atatataaaa ttcaataaat
     8641 taaaaagaac gtattttgaa tcatctatat caaaatacgt tctttttaat ttgtatgtat
     8701 ctggtaaaat gaaatagagg cagaaaattc ttattaataa aggggtaaat atgaaaaagg
     8761 ttaatgttac atacgcgctt ttgtacgata aaacgaacga aaaaatctta atggtaaaaa
     8821 acaaaggaaa aaatgggtct tattacacat taccaggtgg ctcagttaaa tttggggaaa
     8881 cgttagaaga ggcagtcatt cgggaagtaa aagaagaaac agggctacat ataactgtaa
     8941 aggggatttg ttccataagc gaagcctttt tcgaggagag aggccatcat gccattttct
     9001 ttaatttttt aggtgaaata atcggcggag agacatatat atcccgtcca aaagaaatcg
     9061 aagagattac ttggatggaa ttacatatag cagcaccgta tttacgtata cctgaacagt
     9121 tactagattt tttacaaagg aaagaaacag taccttactt ttttaacgga acaatcgttc
     9181 atcaatcttt ataaataaat tatttcattt aaggcgggga aaaaatggag tttatcgtta
     9241 gtcacaaaca tttcactcaa gcactttcag aagtaagtaa agctatatct acaaaagttt
     9301 taattcctat attatctggt atcaaaataa cagccgatca atctggaatt actttaatcg
     9361 caagtaactc gaatgttttt attaaaaaat ttatacctaa ttcaatcgaa gacgaacaga
     9421 ttgcaactat attacaagca ggaactattg ttgtacctgc aaaatatttc attgaaatta
     9481 taaagaaaat gccaagtgat atagtaataa aaagtaaaaa tgagcaaatc attacgatac
     9541 aatcagaaga aatcacatta aacttaaatg gacttccagc aaatgagttt ccgaatgtac
     9601 cacttatagg ggatcatgca gaaatacaag tagaaacaga acaattgatt gaagtgttta
     9661 aacaaactgt ttttgcagta gctaaaaatg aatccagacc tgttctcacc ggagtacata
     9721 tagagttaga tcacaataaa ttagtttgcg ctgctactga ctcacataga ctagctatac
     9781 gtgaaacgcc aatttcttca gatgcgaaag caaattgtat cgtaccaagc gcaactatta
     9841 gtgaacttct aaaattaatg aacagtaata cggaattcgt atacatatac ctttcagaca
     9901 gtcacgttat ttttacattc ggaacaacta cgttatattc aagactcatt gaagggaaat
     9961 atcctaatat atctaatctc attccaaatg aatttcaaac ggttattaac gtagaaagaa
    10021 aaaagatttt acaaggtgta gatcgatcga gcttattagc aagtgaatgg gcaaataaca
    10081 acgtcaactt agaaatcatc aatgaatcca caattaaaat ttcttctaac gcttctcaga
    10141 ttggtaaaat agctgaaaca caacaagtag atgcgattca aggtgaaaaa caattaaata
    10201 tatctttcga cggacgattt atggtagatg ctttaagggc aataacagaa gaaatggtta
    10261 ctttaag
//
