LOCUS       NZ_KB976671            10324 bp    DNA     linear   CON 01-APR-2024
DEFINITION  Bacillus toyonensis strain HuA2-3 acrHg-supercont1.9, whole genome
            shotgun sequence.
ACCESSION   NZ_KB976671
VERSION     NZ_KB976671.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus toyonensis
  ORGANISM  Bacillus toyonensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 4008403)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C.,
            Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T.,
            Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     The Genome Sequence of Bacillus cereus HuA2-3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4008403)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C.,
            Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T.,
            Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2012) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_000399405.1
            Current Name                      :: Bacillus toyonensis
            Previous Name                     :: Bacillus cereus HuA2-3
            Date Updated                      :: 2022-03-10
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_000496285.1
            A1 Name                           :: Bacillus toyonensis
            A1 ANI                            :: 99.4201%
            A1 Query Coverage                 :: 83.75%
            A1 Subject Coverage               :: 94.68%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_006094295.1
            A2 Name                           :: Bacillus cereus
            A2 ANI                            :: 91.7421%
            A2 Query Coverage                 :: 77.41%
            A2 Subject Coverage               :: 80.96%
            ##Taxonomic-Update-Statistics-END##
            ##Genome-Assembly-Data-START##
            Assembly Method                   :: allpaths v. R41865
            Assembly Name                     :: Baci_cere_HuA2-3_V1
            Genome Coverage                   :: 108.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000399405.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 01:17:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,841
            CDSs (total)                      :: 5,738
            Genes (coding)                    :: 5,542
            CDSs (with protein)               :: 5,542
            Genes (RNA)                       :: 103
            rRNAs                             :: 6, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 3, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 81 of 196
            Pseudo Genes (incomplete)         :: 115 of 196
            Pseudo Genes (internal stop)      :: 62 of 196
            Pseudo Genes (multiple problems)  :: 52 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:43:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1751851
            Orig. end                         :: 1762175
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB976671.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            The organism was changed from 'Bacillus cereus HuA2-3' to 'Bacillus
            toyonensis' in March 2022.
            Please be aware that the annotation is done automatically with
            little or no manual curation.
FEATURES             Location/Qualifiers
     region          1..10324
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10324
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10324
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5324](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5324
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(58..71)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.92"
                     /tool="antismash"
     gene            988..2436
                     /locus_tag="IG5_RS16930"
                     /old_locus_tag="IG5_01769"
     CDS             988..2436
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-465). E-value:
                     1.2e-29. Score: 95.4. Matches aSDomain:
                     nrpspksdomains_IG5_RS16930_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="IG5_RS16930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01769"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_000471599.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 9.3e-28, bitscore:
                     95.3, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYFKKMIMKGHLLGDQKIPSTRELSEL
                     LSVSRNTVLSAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTTLADE
                     LDLMKHGVRWEKGMVVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYRPL
                     MKYLLHYMEIKGVDISNKDILITNGFTEGLDIVLSSLAKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMNDDGIDTTEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYKVPIIEDGFNEELRYSGSHLSPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQY
                     IPFKRMTGDGGLHLFIELEEKIHARTLLQKCYEKGVTFSPGDVFYSDGGGANTFRLGFS
                     RLKEKEIVSGIQIIGETLKNETWS"
     aSDomain        1423..2382
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IG5_RS16930_Aminotran_1_2.1"
                     /evalue="1.20E-29"
                     /label="IG5_RS16930_Aminotran_1_2.1"
                     /locus_tag="IG5_RS16930"
                     /protein_end="465"
                     /protein_start="145"
                     /score="95.4"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYRPLMKYLLHYMEIKGVDISNKDILITNGFT
                     EGLDIVLSSLAKKSGRVICENPTHHAALKLFRLHGLEVHGIDMNDDGIDTTEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYKVPIIEDGFNEELRYSGSHLSPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQYIPFKRMTGDGGLHLFIELEEKIHARTL
                     LQKCYEKGVTFSPGDVFYSDGGGANTFRLGFSRLKEKEI"
     gene            2441..3355
                     /gene="ddlB"
                     /locus_tag="IG5_RS16935"
                     /old_locus_tag="IG5_01770"
     CDS             2441..3355
                     /EC_number="6.3.2.4"
                     /codon_start=1
                     /gene="ddlB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="IG5_RS16935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01770"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_002038752.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 8.9e-22,
                     bitscore: 75.6, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 2e-13, bitscore:
                     48.6, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 3.3e-08, bitscore: 31.3,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 2.3e-51,
                     bitscore: 172.1, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 5.4e-06, bitscore:
                     24.0, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSGICMDKNISKKILRY
                     EGVETPDWIELTKMADLKLDDLDKLGFPLVVKPNSGGSSVGVKIVNDKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSIFDGKHLPIVSIRHAAEFFDYNAKYDDVSTIEEVIELPT
                     EIKERVNKASLACYKALKCSVYARVDMMVKDGIPYVMEVNTLPGMTQSSLLPKSADAAG
                     INYSKLLDMIIETSLRVRKEEGF"
     regulatory      3429..3534
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            3662..4351
                     /gene="tenA"
                     /locus_tag="IG5_RS16940"
                     /old_locus_tag="IG5_01771"
     CDS             3662..4351
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="IG5_RS16940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01771"
                     /product="thiaminase II"
                     /protein_id="WP_000144530.1"
                     /transl_table=11
                     /translation="MTFSQTLRKEVDSIWEASFHHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITEEEKGHFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYKEWISAY
                     GSDWFRTLVEEQITRLDTIAELVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDT
                     EVKDVIG"
     misc_feature    complement(4643..4657)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 17.62"
                     /tool="antismash"
     gene            5001..5324
                     /locus_tag="IG5_RS16945"
                     /old_locus_tag="IG5_01772"
     CDS             5001..5324
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108508.1"
                     /locus_tag="IG5_RS16945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01772"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001032074.1"
                     /sec_met_domain="TIGR03601 (E-value: 7.4e-37, bitscore:
                     123.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKFQQELQALSLNDYQSGNIVYWDQQNQYPYYYFEDAARRCGGC
                     GRCGGCGGRCGGGGFRCGGFRCIGCFGCFGCGGCGGCGGCSNCFNGFNGTTDTTGTIVT
                     YEY"
     gene            complement(5480..5752)
                     /locus_tag="IG5_RS16950"
     CDS             complement(5480..5752)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088127.1"
                     /locus_tag="IG5_RS16950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000088127.1"
                     /transl_table=11
                     /translation="MSRKSDITAKDRKDAGKKALKSAVVKGWINVGKWGFFGDMVAFMD
                     RYYTGYKNYNLKKKRERRADSSSSFWFWCNKSAYKSQRVRQEYKT"
     gene            complement(6190..6537)
                     /locus_tag="IG5_RS16955"
                     /old_locus_tag="IG5_01773"
     CDS             complement(6190..6537)
                     /GO_component="GO:0043592 - exosporium [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343262.1"
                     /locus_tag="IG5_RS16955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01773"
                     /product="exosporium leader peptide-containing protein"
                     /protein_id="WP_000343263.1"
                     /transl_table=11
                     /translation="MDEFLSSAALNQNSIGPTLPPMQPFQFPTRPTGNTGPPGIVSVTF
                     NQSHIPATSPTNLVLPAISVGVNQIVKLEGVFRSDYVSLSPLTEFTVSSILALKRGVFT
                     IAVQMELQEDI"
     misc_feature    6661..6677
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 22.69"
                     /tool="antismash"
     misc_feature    complement(6661..6677)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.06"
                     /tool="antismash"
     misc_feature    7527..7545
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.44"
                     /tool="antismash"
     gene            complement(7645..7944)
                     /locus_tag="IG5_RS16960"
                     /old_locus_tag="IG5_01774"
     CDS             complement(7645..7944)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110270.1"
                     /locus_tag="IG5_RS16960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01774"
                     /product="hypothetical protein"
                     /protein_id="WP_000110270.1"
                     /transl_table=11
                     /translation="MSVFINYAITPKVFNPNLTSLPNNRRSFIPTQKESSKNICYLLET
                     YYLGKKIKQLLVNGTILKASIFVGFHNRSRLATFIDENGDITVVNCERIDAILL"
     gene            complement(8025..8246)
                     /locus_tag="IG5_RS16965"
                     /old_locus_tag="IG5_01775"
     CDS             complement(8025..8246)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807684.1"
                     /locus_tag="IG5_RS16965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_01775"
                     /product="hypothetical protein"
                     /protein_id="WP_000807684.1"
                     /transl_table=11
                     /translation="MAEHTKCVCEQLERLQLGTEVDVFLTGTTLEDLIFTNFNPDNFCV
                     TFVDNTTEPGSIIVLDCRDIQALRIEAE"
ORIGIN
        1 attggtagaa tcaacagtta caaatcttaa agatggaaat ccgggaaaaa aatatataaa
       61 acaagcgatt gagaaacata tggatattgt tgcaatttca aaaggtgcac tcgttactaa
      121 ctggagagaa ataaaggcag cagcaaaaga agcgaatgta cgaattcgct atagtggcgc
      181 aactgctgca gcactaccga cattagatat tggacaattt agtttggctg gatgcaatat
      241 tgaaaaaata gaaggaatat taaacgggac aactaattat attcttacaa aaatgtatga
      301 agaagatgtt acgtttgaag aagcactgaa agaagcgcaa aataaaggaa ttgctgagac
      361 gaacccaata ttagatgtaa gtggttcgga tagtgcgtgt aaattattac ttttggcaaa
      421 cagcttgatg ggtgttgaga atatgcttac tgatatgcat ataaaaggaa tcgaaaatgt
      481 tacaaaacaa caaattcgac atgctaagga acaaaacaat tatattaaat taatagcttc
      541 agcatataag gatatggatg gtaaagtgaa tcttaatgta gaaccgcttg aaatacaaaa
      601 agaccatcca ctagcaaaag taaatggaac tgaaaaagga ataacattct ttacagatac
      661 aatgggacaa gttactacaa ttggtggagc ttctaatcca cgcggagccg cagcagctgc
      721 tttaaaagat gtaattaact tatatcgtag agatttatag aagtaaaact cacaccttca
      781 aaattcggat ggagaaaaaa agttaggtgg gagcctttta gcccatatat gtccgattcg
      841 ggctaaaaat cggtggggga tgaataaaat tcccacggat taaagtttca ctttataaga
      901 ccgtaccgct gttaataaaa aatacatatg tagtggacct tttgagaatg aaataaatat
      961 gcaaaaataa ttgcaggggg taagaaattg tttaaagatt ttaaagttgt aaaagatcgt
     1021 cccgtttata ttcaattaaa ggattatttt aaaaagatga ttatgaaagg gcatttgctc
     1081 ggtgatcaaa agatcccatc aacaagggaa ctgagtgaat tattatcagt gagcagaaat
     1141 acagtactct ctgcttatgc ggatttagaa caagaaggac tcatttatgc agtaaaagga
     1201 aaagggaatt tcgttgcgaa ggtggatata tctaacactt cgtctgttga aatagattgg
     1261 aaaaataaac ttaatacagt taccacatta gcggatgaat tagacttaat gaagcatgga
     1321 gtccgctggg aaaaaggaat ggttgttttt aatagtatag ccccggacga aaagctattt
     1381 gatgttgaaa atttcaaaag agcttttctt actcgtatgt ccattgaagg ggatatcgta
     1441 ttgaactacg gatatgcaaa aggttaccgc ccgttaatga aatatctact tcattacatg
     1501 gaaataaaag gtgtagatat ttcgaacaaa gatattttaa ttacaaatgg atttacagaa
     1561 ggattagata ttgtactatc ttccttagcg aaaaaatcag ggcgtgtgat ttgtgagaat
     1621 ccaactcacc atgctgcatt gaaacttttt cgcttacatg gacttgaagt tcatgggata
     1681 gatatgaatg atgatggtat tgatacgact gaagtagaaa aaagtttacg tgaaaaggat
     1741 tttgattttg cgtatttaat tccttcttat cataatccaa ctggtattgt tacaagttcg
     1801 gagaaaagaa cggaattgat gaggttattt tcgaaatata aggttcctat tatagaggac
     1861 ggatttaatg aagaattacg ttattcaggt tcacatttat cgccactatt aacttttgca
     1921 ggggctggta ataatgtgat ttatattagt agcttttcga aggtactttt ccctggttta
     1981 cgtgtagggt ggattattgc cgataaagaa ctgattcatt atttagaaag tgttaaaaga
     2041 gcaagaacca ttcacacatc tacgttagat caagctgttt tgtttcaata tttacatgaa
     2101 ggatattttg aaaaatattt aaaaaaggca agatctgttt acaagaaaaa atatgagtta
     2161 gccgttgggg cttgcaatca gtacattccg tttaaaagga tgactggaga tggcggactt
     2221 catttgttta tagagttgga agagaaaatt cacgctcgca cacttttaca aaagtgttat
     2281 gagaaaggtg taacgttttc tcctggagat gttttttatt ctgatggagg gggagcgaat
     2341 acattccgct taggtttttc acgtttgaag gagaaagaaa tagttagtgg gattcaaata
     2401 attggtgaaa ctttaaaaaa tgaaacttgg agttgagaaa atgagaattg gcgttattat
     2461 gggaggagta tcctctgaaa aacaagtatc aattatgact ggaaatgaaa tgattgcaca
     2521 tttagataaa aataagtatg aaatcgttcc aattacgtta aatgaaaaaa tggatttaat
     2581 tgaaaaagcg aaagacattg attttgcatt gctagcatta cacggaaaat acggagaaga
     2641 tggtacggtt caagggacgc ttgagagttt aggtattcca tatagtggca gcaatatgtt
     2701 atctagcggt atatgtatgg ataaaaatat ttcgaaaaag attttacgtt atgaaggagt
     2761 agaaacacca gattggattg aacttacaaa aatggcggat ctaaagcttg atgatttaga
     2821 taagttagga tttccgttag tggtaaaacc aaactctggc ggctcgagtg ttggcgtaaa
     2881 gatcgtcaat gataaaaatg aattgatctc aatgctggaa actgtattcg aatgggattc
     2941 tgaagtagta attgagaagt atataaaagg tgacgagatt acttgttcaa tttttgatgg
     3001 taaacattta cctatcgttt caattcgaca tgcagctgag ttttttgact acaatgcaaa
     3061 atacgatgat gttagtacaa ttgaagaagt tattgaactt ccaactgaaa ttaaggaacg
     3121 tgtaaataaa gcgtcattgg cttgttataa agcattaaaa tgtagtgttt atgcaagggt
     3181 tgatatgatg gtgaaggacg gaattccata tgtaatggag gttaatacat taccagggat
     3241 gacccaatca agtttactgc caaaaagtgc agatgccgcg gggattaatt atagtaaact
     3301 attagatatg ataattgaaa cttcattaag agtgaggaaa gaagaaggtt tttaagataa
     3361 ttatacaatt aaaacttttg atagttccac gaagtatgct atgataatta caatacgaca
     3421 actaaatatt tatccactag gggggccttt tgtaggctga gatcaaacat aattttgaga
     3481 ctcttagtac ctgatctggt taataccagc gtagggaagt gggaagacga ttatatttaa
     3541 agaatacatt aatattgtct atttcacttt ctttacgcct gtaaagaaag ttttttttgt
     3601 tttacagatt taataactgt ggataaatac atatatttta tacattattg gaggaattta
     3661 aatgactttt tcacaaacat tacgtaaaga agtagattct atttgggagg caagttttca
     3721 tcatcctttt gtaaaaaaac ttggtgaagg tacgttagat ttggcaagtt ttcgttatta
     3781 cgtacttcaa gattcatatt atttaagtca ttttgctaga gtacaaactt taggagctgc
     3841 aaaggctctt gaattagaaa cgacagctcg catggcacac catgctcaaa atacatacga
     3901 ggcagaatta tcattacatg agaatttcgc aaagaaatta ggaattacgg aagaagaaaa
     3961 aggtcatttt atccctgccc caactgcata tgcgtatact tcacatatgt accgtgcggc
     4021 gtatgaaggg catttagggg atattatcgc agcaatttta ccttgctact ggctatatta
     4081 tgaaattggt gaacgtttaa aagaatgtca gccagaagag ccgatttata aagagtggat
     4141 ttctgcttat ggatctgatt ggttccgtac tttagttgaa gagcaaatta cacgattaga
     4201 tactatcgct gaactagtga cagagtctga tcggaagcgt atgaaacagc atttcattat
     4261 tagtagtcaa tatgaatatt cattttggga aatggcttat acattagaaa aatggccagt
     4321 ggatacagaa gtaaaagatg taattggata aagagtagag gtattgttat acatatgtat
     4381 ggataatacc tctttatatg atttcaaaaa aggggtatcg caaaaaagac gtatcaatct
     4441 aagtgcgtct tttttcaatt ttcttcaatt agcaatttca tggagagctt gctgaaaaag
     4501 tagaatgaaa agccctaatc agggcttttt ttgtaggaat ttcttaaccg tcattgagtc
     4561 ctgtcactag taggtgtttt gacgctacga cggactaaat cgattattcg gctagtgaag
     4621 tatataaaaa agttaggaat atctgaaggt gctctcagaa agtatatgga agtgttataa
     4681 cgagaggcat ttacagtaca gaaaaatcta agcggcatac gggaatacgt aggatacggc
     4741 atcatggtta ttgagaaatg aattgaactt agtaagcatg aagatatgac gcaagcattc
     4801 acattttata atttctattt ataaaaatga gcccatagta aaaataggta tatgaacaag
     4861 gtgcaccgtc tagtgatagg tcatacttta taggtgtacg tattgaactt agtttatagc
     4921 gttatttcta cttagattta acgaacagaa atattggaaa gcatcgatga ggttaaagtt
     4981 tataaacgga ggtcatgaaa atgaataagt ttcaacaaga actacaagcg ttaagcctta
     5041 atgattacca gtctggaaat attgtgtatt gggaccagca aaaccaatat ccatactact
     5101 attttgaaga tgctgctcgt cgctgtggcg gttgcggtcg ttgtggcggt tgtggaggtc
     5161 gttgtggtgg tggtggattc cgttgtggtg gattccgttg tattggttgc ttcggttgtt
     5221 ttggttgtgg cggctgtggc ggctgtggcg gttgttcaaa ttgttttaat ggttttaatg
     5281 gtaccactga tactactggt actattgtaa catatgaata ttgataagag agaaaaggaa
     5341 tctactttgt aaagcgttat acaattttat aaaagagata aatcaaaaca cacgtagcat
     5401 cttttaattt agagagataa tttatccaac cttttataaa cattgtgact ttatttcaaa
     5461 tcaaaaatcg aatgtctttt tatgttttat actcttgacg cactctttga cttttataag
     5521 cagatttatt gcaccagaac caaaaagaag agctgctatc agcccttctt tctcttttct
     5581 ttttaagatt gtaattctta tagcctgtat aatatctgtc cataaatgca accatgtcac
     5641 caaagaatcc ccatttacct acatttatcc aacctttaac aactgccgat tttaatgctt
     5701 ttttccccgc atctttacga tcttttgctg ttatatctga ttttctgctc attctcatac
     5761 tttatatgaa atatgtgaaa atcagtatga atggtggaag tgagcgcaac tttaaaagct
     5821 atttattggg attaacaaat ttaataatct atttttatta ctattgatta attttgaaac
     5881 gtaacaaaac tacgtaactg tttgtataaa atgcactgct ctagtattga aaatttagaa
     5941 catggaatag ggtgctttgt ttctacaagc tgtttttgtt ttacttcata ttacgattta
     6001 tagaccggga attacacttt aaatgtttat tactgtagca gttatgtttc tttcactaat
     6061 agtgttagtg gatgtaccaa ttcccgtggc attcagagat agcctgtata cataagtacc
     6121 agggggaggc gtatctatcc agactattga aacgttgtgt gttaaatttg ttgaaacatt
     6181 tactggttat taaatatctt cttgaagctc catctgaacc gcaatagtaa agacgcctct
     6241 ttttagagcg agaattgaag atacagtaaa ttctgtaagg ggacttaggg aaacataatc
     6301 agatctgaaa actccttcta actttactat ttgattaacg cctacactaa tagctggtaa
     6361 tacgaggtta gttgggctag ttgcaggaat atgactttgg ttaaatgtaa ctgaaacaat
     6421 gccaggtggt ccggtgttac cggtcggtct agtagggaat tgaaaaggtt gcataggtgg
     6481 gagtgttggt ccaatcgaat tctggtttaa tgcagcagaa gataaaaact cgtccaatat
     6541 aattcacctc taaaaaattt atattactga tagtaaatgt aggcgttgag caaagtgaca
     6601 tggacaagcg gttgtatgtt aaaaattaaa caaaatagtt attttattaa agaaaaaaca
     6661 tgcacaaaaa ttgcgcatgt ataataaaaa gctatttctt gtctatgtaa atatatgatt
     6721 ggcttataca aaccgtgagt gtgtttgaga aaaatggtta tttattaaca aatataaaga
     6781 gcgcttttaa ggcgctcttt aaccaaaact aatattaaaa aagaataccg catgatattt
     6841 tatgtatgtt cttaataaat gtgcggtttt taaacaaaat ccttatttta tcttaaataa
     6901 aaagagcaca catataagcg tgctctcaaa taagaaaggt agaatgctat gaatggaaag
     6961 cctcgataga ataacatata cttgtccggt ttaaatgtga gaagttttta ataaaaacgc
     7021 tattttagta aggaacaagt ttaaaaggcc ctgattgaca caggaccttt taaaagggaa
     7081 caacacttga catgttaaaa attatgactg aaaggtattt tcatattacc attagtgggg
     7141 ttttaattcc aataatggtt gttgagaaat aaaaccttta ttttgcacaa caaagcagct
     7201 agctgaagta gctaattgct ttattgtaca attttagaag ttcactggat acagatatat
     7261 gttgtaacaa atagttacat ctatggtata aacaaatacc aaaatattat gcaggaaaga
     7321 aaactaaatt aaaacttcat tttataaaga gagaagcaca aaatattata tgtgtatcca
     7381 gtcaattgag tgcgttttta ctaacaaaga gcatctagca aaagctaact gttcggttct
     7441 ccaaggggga acaaggagaa agtaacttaa tgggttgtct acattattga cggaatctgg
     7501 aggcttattc ggtcaaataa aaaaggtact gtatataagt gcactttgga ccaagttttt
     7561 tatggtaaca gttattggta ttatgtgtaa gaactaacgc gtatcttgaa aataatgatt
     7621 atttatttaa atgtaaattc ataattagag tagaatcgca tcaatacgct cgcagttaac
     7681 tacagtgata tctccgtttt catctataaa tgtagcaagg cgggatctat tatggaatcc
     7741 tacaaatata gaagctttta atattgtgcc gttaactaac agctgtttta ttttttttcc
     7801 taagtaatat gtttctaaaa gatagcaaat atttttagaa ctctcttttt gggttgggat
     7861 aaaagaacgt cggttatttg gaagagatgt tagattagga ttgaatactt tgggagtaat
     7921 agcatagttg atgaaaactg acatttttcc ggctcctcta ttttataatt tatattgaaa
     7981 tatgctgtgt gaaatagggc ttttttcaaa gccccatctt tactctactc agcttcaatt
     8041 ctaagcgctt gaatatcacg acagtccaaa acaattatag agccaggttc tgttgtgtta
     8101 tccacaaatg ttacacaaaa attatctgga ttaaagttag taaaaataag atcctctagt
     8161 gttgttcctg taaggaaaac atctacttct gtacctaact gtaatctttc taattgttcg
     8221 caaacacatt ttgtatgctc agccatataa aaacacctcc ttttctcaaa ttactattaa
     8281 tatatgtata aattaaatag aacgattgga gtaatagctt agtaatatta attattttgc
     8341 tagttaatat ttttataaaa taatcctttg aaatgaatat cgtccggcta gaaaactaga
     8401 ggacaccaat tcattaaagc agcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnn
//
