LOCUS       NZ_KB976670            10288 bp    DNA     linear   CON 01-APR-2024
DEFINITION  Bacillus toyonensis strain HuA2-3 acrHg-supercont1.8, whole genome
            shotgun sequence.
ACCESSION   NZ_KB976670
VERSION     NZ_KB976670.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus toyonensis
  ORGANISM  Bacillus toyonensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 24480)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C.,
            Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T.,
            Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     The Genome Sequence of Bacillus cereus HuA2-3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24480)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C.,
            Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T.,
            Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2012) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_000399405.1
            Current Name                      :: Bacillus toyonensis
            Previous Name                     :: Bacillus cereus HuA2-3
            Date Updated                      :: 2022-03-10
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_000496285.1
            A1 Name                           :: Bacillus toyonensis
            A1 ANI                            :: 99.4201%
            A1 Query Coverage                 :: 83.75%
            A1 Subject Coverage               :: 94.68%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_006094295.1
            A2 Name                           :: Bacillus cereus
            A2 ANI                            :: 91.7421%
            A2 Query Coverage                 :: 77.41%
            A2 Subject Coverage               :: 80.96%
            ##Taxonomic-Update-Statistics-END##
            ##Genome-Assembly-Data-START##
            Assembly Method                   :: allpaths v. R41865
            Assembly Name                     :: Baci_cere_HuA2-3_V1
            Genome Coverage                   :: 108.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000399405.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 01:17:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,841
            CDSs (total)                      :: 5,738
            Genes (coding)                    :: 5,542
            CDSs (with protein)               :: 5,542
            Genes (RNA)                       :: 103
            rRNAs                             :: 6, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 3, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 81 of 196
            Pseudo Genes (incomplete)         :: 115 of 196
            Pseudo Genes (internal stop)      :: 62 of 196
            Pseudo Genes (multiple problems)  :: 52 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:43:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 13032
            Orig. end                         :: 23320
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB976670.1.
            Please be aware that the annotation is done automatically with
            little or no manual curation.
            The organism was changed from 'Bacillus cereus HuA2-3' to 'Bacillus
            toyonensis' in March 2022.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10288
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10288
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10288
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5288](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5288)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    19..34
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 23.27"
                     /tool="antismash"
     misc_feature    complement(20..33)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 21.19"
                     /tool="antismash"
     gene            72..1193
                     /gene="tnpB"
                     /locus_tag="IG5_RS08130"
                     /old_locus_tag="IG5_05784"
     CDS             72..1193
                     /codon_start=1
                     /gene="tnpB"
                     /gene_functions="other (smcogs) SMCOG1192: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090861.1"
                     /locus_tag="IG5_RS08130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05784"
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_016090861.1"
                     /transl_table=11
                     /translation="MMINKAYKFRIYPNKAQATLINKTIGCSRFVFNHFLSLWDNAYKE
                     TGKGLTYGTCSAKLPAIKKEFVWLKEVDSIAIQSSVRNLADAYTRFFKKQNSAPRFKSK
                     KNNVQSYTTKQTNENIAVVGNKIKLPKLGLVRFAKSREVQGRIVNATVRRNPSGRYFVS
                     LLVETEVQELPKTHSYIGMDVGLKDFAILSDGTTYKNPKFFRSLEEKLAKAQRVLSRRM
                     KGSSRWNKQRVKVARIHEYMTNARKDYLDKISTEIIKNHDVIGIEDLQVSNMLKNHKLA
                     KAISEVSWSQFRTMLEYKAKWYGKQIIVVSKTFASSQLCSCCGYQNKDVKNLNLRKWDC
                     PSCLTHHDRDINASINLKNEAIRLLTARTVEIA"
     gene            complement(1972..2598)
                     /locus_tag="IG5_RS08135"
                     /old_locus_tag="IG5_05785"
     CDS             complement(1972..2598)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402086.1"
                     /locus_tag="IG5_RS08135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05785"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000402086.1"
                     /transl_table=11
                     /translation="MEIELKNISKKYENKQVLDNFNLVVNKGEMMAITGQSGTGKSTLL
                     NIVGLLEEPDSGDVIIQGIENAWKSEKKQIELFRYTIGYLFQNYALIDNETVSKNLDVA
                     LEYVKLSNKDDKKKEVLEKVGLLDKLNSKIYQLSGGEQQRIALARLMLKKNDIILADEP
                     TGSLDEVNRDQVLSILKSLNNEGKTILIVTHDPEVSKICTNVVTL"
     gene            complement(2604..4823)
                     /locus_tag="IG5_RS08140"
                     /old_locus_tag="IG5_05786"
     CDS             complement(2604..4823)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002154969.1"
                     /locus_tag="IG5_RS08140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05786"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_002154969.1"
                     /transl_table=11
                     /translation="MKKILITLVLVAYAVSSFVFTKHFEKREMVNINFSDRNGIQVVEL
                     NKDERPEDKLRKIEDLSKKEKINIYKLSYKPIKTSKKQKVIIYAAIGDETQFKQKYPLE
                     WGKIFNQDHSVASYLSSRDTKDKEQVGKINIFSPELMVEIKPIEAAKDENLRGAYFVDS
                     NNPNVVQKVKEKLNTEIGFKTEESERAGAMNILENQGVDKYFIIVLTIVLLLIFLSFLY
                     YILLNYKSLAIRKLFGYTDRELIFKHLLKESIQIHTLSLMVVIIGQVIYLFFYNGMSKF
                     LDFGSTWIVWQLIFSGISILVSLLPSLMIYHIKIMELLKNKKPLKLVQFLNYSSKFIFS
                     IFLIIFSFNLINYYQDLTVQNSNQEQWDTTKNYSFYEYQSNIIGDRTNWEYETGIKSKK
                     LFGITNQKGALLIKPSDGITYKKQLEYNNSLNNTIGDVKEYDPHGGNTLQVNSNYLHDN
                     PVYDLNGKKINIDADYGDYLVILVPNKYRSNEADILKMYKEWYQFTRFIDEDKHKESIG
                     EKIEPHKQVEIKLTYIQDNQKHFLYNPMLEVKNQNYSNGAILIVVNSENMGGDSYLSYL
                     TGRYFFPKVDNPSQPYKELKEDIKIAGLQDIILTTPSLYSIVDDYLFKLKSELQFTLLF
                     VILLLSIEIIITCFTILNYLERNKFIHAVQRINGFDFYKRHSKICFLLSLFWLIIYLGA
                     LTFKLATYTIISLIIPTYWLVELLIVYFIIRFVEKKKNKDVLKGA"
     misc_feature    4776..4796
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 26.98"
                     /tool="antismash"
     gene            complement(5001..5288)
                     /locus_tag="IG5_RS08145"
                     /old_locus_tag="IG5_05787"
     CDS             complement(5001..5288)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768886.1"
                     /locus_tag="IG5_RS08145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05787"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000768887.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 3.4e-18,
                     bitscore: 56.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLKKTVSSLVLSAAILGSGATAAFAATQYVGGGTWNYGTGGGSV
                     WSNYYHGSKVHKSSVIGQYYYSSPWTNPGYTSYASAPDTAGVDESYWSVK"
     misc_feature    5540..5553
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.97"
                     /tool="antismash"
     gene            5942..6076
                     /locus_tag="IG5_RS31755"
                     /old_locus_tag="IG5_05788"
     CDS             5942..6076
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653179.1"
                     /locus_tag="IG5_RS31755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05788"
                     /product="hypothetical protein"
                     /protein_id="WP_000653179.1"
                     /transl_table=11
                     /translation="MKDIYELLQEIHINLTEFEERKCSEVEKEKVKGFVKELIKKKED"
     gene            complement(<6231..>6339)
                     /locus_tag="IG5_RS31860"
                     /pseudo=""
     CDS             complement(<6231..6339)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259872.1"
                     /locus_tag="IG5_RS31860"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="DDE-type integrase/transposase/recombinase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MS"
     gene            complement(6382..6669)
                     /locus_tag="IG5_RS08150"
                     /old_locus_tag="IG5_05789"
     CDS             complement(6382..6669)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1058: ArsR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016107136.1"
                     /locus_tag="IG5_RS08150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05789"
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000935902.1"
                     /transl_table=11
                     /translation="MLNYEKDAEVFKALAHPVRIEILKELIIRGACNVTQIVGILGIPQ
                     STVSQHLAKMKSQKLIRNDRKGLEVYYSAQNKTINSIVEILLDYKSTNCK"
     misc_feature    complement(7062..7076)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.82"
                     /tool="antismash"
     gene            complement(7219..7530)
                     /locus_tag="IG5_RS08155"
                     /old_locus_tag="IG5_05790"
     CDS             complement(7219..7530)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637520.1"
                     /locus_tag="IG5_RS08155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05790"
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="WP_000637486.1"
                     /transl_table=11
                     /translation="MIVTTTNTIQGKEIIEYIDIVNGEAIMGANIVRDIFASVRDVVGG
                     RSGAYESKLKEARDIAMEEMKQLATQKGANAIVGIDVDYEVVRDGMLMVAVSGTAVRV"
     gene            complement(7794..8489)
                     /locus_tag="IG5_RS08160"
                     /old_locus_tag="IG5_05791"
     CDS             complement(7794..8489)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207761.1"
                     /locus_tag="IG5_RS08160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05791"
                     /product="response regulator transcription factor"
                     /protein_id="WP_000207756.1"
                     /transl_table=11
                     /translation="MTTILVLEDDITIRSFITLNMKRAGFNVLETDTGEKALELLEQHN
                     VDIVILDVMLPGIDGFRVCKSIRKNNEDIGIIILTALVQEKDQVHGLTIGADDYIKKPF
                     SIIELVARVQSLLRRIQKNKKDLELIHSGPFQLNLLLGELYRNEIVIDLTPTEHIILRY
                     LMENSMKPISRNELLNKIWGINCVGNTKVIDVNINRLRQKIETSPSNPEFLLTVRGKGY
                     RWNENKHCN"
     gene            complement(9056..9337)
                     /locus_tag="IG5_RS08165"
                     /old_locus_tag="IG5_05792"
     CDS             complement(9056..9337)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053163.1"
                     /locus_tag="IG5_RS08165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05792"
                     /product="hypothetical protein"
                     /protein_id="WP_000053163.1"
                     /transl_table=11
                     /translation="MSLITPHYLLVGGCSKDKVLQAHKKAKEIFNPNNEMNKLISPLKS
                     DSFFILYDDSHHSWKNEDEYMKAKTAYIQYLVESDIQFVEMATQELIS"
     gene            complement(9590..9712)
                     /locus_tag="IG5_RS31760"
                     /old_locus_tag="IG5_05793"
     CDS             complement(9590..9712)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002050085.1"
                     /locus_tag="IG5_RS31760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05793"
                     /product="hypothetical protein"
                     /protein_id="WP_002050085.1"
                     /transl_table=11
                     /translation="MIASNGIVDIHSKKQAQEFTMEMTKQKLHETVGFEWGRTT"
     gene            complement(9742..9897)
                     /locus_tag="IG5_RS31065"
                     /old_locus_tag="IG5_05794"
     CDS             complement(9742..9897)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056873.1"
                     /locus_tag="IG5_RS31065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05794"
                     /product="hypothetical protein"
                     /protein_id="WP_000074837.1"
                     /transl_table=11
                     /translation="MSPPTQKKMLQRVKKEATSYCNVDPLAFAIGIRNGFLMVMPFWLV
                     VLYLVI"
     misc_feature    complement(9884..9898)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 18.58"
                     /tool="antismash"
     misc_feature    9885..9899
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 18.95"
                     /tool="antismash"
ORIGIN
        1 ctgttacttt actacctatc gaatgtatgt ttgttgtatg atagacacat attgaaatgg
       61 gggtgaaacg aatgatgatc aataaagcat ataaatttcg tatctatcca aataaagcac
      121 aagcgaccct aatcaataaa acgattggct gttctcgttt tgtattcaat cacttcctat
      181 ctctatggga taatgcatac aaagaaacag gaaaaggttt gacatatggt acatgctctg
      241 ccaaacttcc tgccataaag aaagagtttg tttggctaaa agaagtggat agtattgcga
      301 ttcagtcatc tgtccgcaac cttgcggatg cttatacacg ctttttcaaa aaacaaaaca
      361 gtgccccgcg tttcaaatct aagaaaaata acgtgcaatc ttataccaca aaacaaacaa
      421 atgaaaacat tgccgttgta ggaaacaaaa ttaaattgcc aaaactaggt cttgttcgat
      481 ttgccaaaag tcgtgaagta cagggacgta ttgtaaatgc tacagttaga cggaaccctt
      541 ctggcagata ctttgtgtca ttgttagtcg aaacagaagt gcaagaactt ccgaaaacac
      601 attcttacat tggaatggat gtgggactaa aagatttcgc cattttgtca gatggaacaa
      661 cctataaaaa tccgaagttt tttcgatcat tagaagagaa gttggcaaaa gcacagcgtg
      721 ttctttctag aagaatgaaa ggatcttctc gctggaataa acaacgagta aaagtagcta
      781 gaattcatga atacatgacg aatgctagaa aagattactt ggacaaaatc tcgactgaaa
      841 tcatcaaaaa ccacgatgtt atcggtatag aggatttgca agtatcgaat atgttaaaga
      901 atcataagtt agcaaaagca attagtgagg tatcatggtc acagtttcga actatgttgg
      961 aatataaggc aaaatggtac ggcaaacaaa tcattgtcgt atcgaaaaca tttgcttcaa
     1021 gccaattatg ttcttgttgt ggatatcaaa acaaagacgt taaaaatcta aacctacgta
     1081 aatgggactg cccttcttgc cttacacacc atgataggga tattaacgca agtatcaatc
     1141 taaagaatga agcgataagg cttctaaccg caaggactgt ggagatagcc taataaatta
     1201 gagttcgata gaactcttta cttaggaatc cccctcttct aaacgaagtg aaagtggggg
     1261 tagttcaatg tttattttca ctaattctcc ataattgaat cttagccaaa ttaatattcc
     1321 tataacctat ccctagaaac ctgtataaaa ttaaatggtt taagtacaga agaaataggt
     1381 acattacttc taatatgaat tagaaactga aatctgtttg tttattatcc atatgtacga
     1441 attccattgc tatcaagcta atattttgaa gcatccccaa aacaaaaaaa ttgtatattt
     1501 ttatgtggag atgccaattt tctcgttttg gggtatttac tttcttaagt tgatgggcat
     1561 gtggttttag gcttatcacg aaattgcgtc aattcaaata gttgcacaaa aacgaaacct
     1621 ttctgactgg gaattaaagg acatctaccg gaaaaggcta aagcctcagt taaaatgaag
     1681 ttgaatactg gagaataaag ccactaaaaa aaagaatgcc ctttttgtta tttaaaagag
     1741 cattcttttt tataagataa gcgtacaaag gtttaaagaa tttcttgtta tgagagttag
     1801 tttttggatc tttatgtttc tcatctatgg cgaaagcctg ttacaataga actaagtcaa
     1861 atatgaaata cccttaggca gctctcatag gtataaaatc tatgggggat ttttcattac
     1921 agttattttg aaatttttgt accataaccg ttaatatctt attttcaaaa tttatagtgt
     1981 aacaacgttc gtacatattt tagacacttc agggtcatga gtaacaatta gaattgtttt
     2041 tccttcgtta tttaatgact ttaatatact aagcacctga tcccgattca cttcatctaa
     2101 tgatccagtt ggctcatctg ctaggatgat atcattcttt tttaacatga gcctagctaa
     2161 tgctattctt tgttgttctc caccactaag ttgataaatt ttactgttta atttgtctag
     2221 tagcccaact ttttctagaa cttctttttt cttgtcatct ttattagata acttaacata
     2281 ttctagtgct acatctaaat ttttgctcac agtctcatta tcaattaatg cataattttg
     2341 aaagagatag cctattgtat atctaaaaag ctcgatttgc tttttctcgc tcttccaagc
     2401 attttctatt ccttgtatga ttacatctcc agagtctggt tcttctaaaa gacctactat
     2461 atttaatagc gttgattttc cggttccact ttgacccgta atagccatca tttctccttt
     2521 atttacaacc aaattaaaat tatcaagcac ttgtttattt tcatatttct tagatatatt
     2581 ttttagctca atttccactt gaactacgct ccttttaata cgtccttatt tttcttcttt
     2641 tctacaaatc gaatgatgaa ataaacaatt aataattcaa ctaaccaata agttggaatt
     2701 ataagtgaaa taattgtata agttgctaat ttaaatgtta atgcacctaa ataaattatt
     2761 aaccaaaaaa gacttaagag aaaacagatt ttactgtgtc gcttataaaa atcaaagcca
     2821 ttgattcttt gaacggcatg aataaattta tttctttcta aataatttaa aattgtaaaa
     2881 caagtaataa taatctctat gctaagtaat aatattacaa aaagaagagt aaattgaagt
     2941 tcgcttttta atttaaagag ataatcatct acaatactat ataatgacgg cgttgtaaga
     3001 atgatatctt gaagacctgc gattttaatg tcttctttta attctttata aggctggctt
     3061 ggattatcta cttttgggaa aaaataacgc ccagttaaat aacttaaata gctgtctcca
     3121 cccatatttt ctgagttaac aacaatcaat atagcgccat tgctataatt ttggttttta
     3181 acctctagca taggattata caaaaaatgt ttttggttat cttggatata ggtaagttta
     3241 atttcaacct gtttatgtgg ttcaattttt tctcctatgc tttccttatg tttatcttca
     3301 tcaataaacc ttgtgaattg ataccattct ttatacattt ttaaaatatc agcttcattg
     3361 ctgcgatatt tattgggaac taatataact aaataatcac cataatcagc atcgatattg
     3421 atttttttac cattcaaatc ataaactgga ttatcatgta aataattaga attcacttga
     3481 agggtgttac cgccgtgagg atcatactct ttaacatctc ctatagtatt gtttaaagaa
     3541 ttgttatact ctaactgttt tttgtacgtt atgccatctg atggttttat aagcaaagcc
     3601 cctttttggt tagttattcc aaatagtttc ttacttttaa ttccagtttc atattcccag
     3661 tttgttcggt cgcctataat attactttga tattcataaa aactataatt ttttgttgta
     3721 tcccattgtt cctggttaga gttttgtaca gttagatctt gataataatt tataagatta
     3781 aaagaaaaga taattagaaa aatcgaaaag ataaactttg aactataatt taaaaattga
     3841 actagtttta atggtttttt attttttaat agttccataa tttttatatg atatatcatt
     3901 aaagaaggaa gtaggctaac tagaatagaa atacctgaaa aaattaattg ccacacaatc
     3961 catgtactac caaaatctaa gaatttactc atcccattat aaaaaaacaa gtatattact
     4021 tgtcctataa taactaccat taaggataac gtatgaattt gaatactttc ttttaataga
     4081 tgcttaaaaa tcagttctct atccgtatat ccaaacagtt ttctaattgc caaactttta
     4141 taatttaata atatataata taaaaaactc aggaagatga gtaacagaac tatagtaaga
     4201 actataataa agtacttatc aactccctga ttttctagga tgttcatagc acctgctcgt
     4261 tctgactctt ccgttttgaa tccgatttcg gtatttaatt tctcttttac cttttgaaca
     4321 acgtttggat tgttactatc aacaaaataa gcgcctctca aattttcatc ttttgccgct
     4381 tctataggtt taatttctac cataagttca ggtgaaaata tgtttatttt tccaacctgc
     4441 tctttatctt tcgtatctct tgatgacaaa taacttgcta cggaatgatc ttgattgaat
     4501 atctttcccc attctagtgg atatttttgt ttgaattgtg tttcatcccc tatagctgca
     4561 tagataatta ctttttgttt ttttgaagtt ttaatcggtt tatatgataa tttatagatg
     4621 ttgatttttt cctttttgga taaatcctca atctttctca gtttatcttc cggacgttca
     4681 tctttattca attcaaccac ttgaattcca tttctatcag aaaaattaat atttaccatt
     4741 tctctttttt caaagtgctt agtaaataca aatgaagata cagcataggc tacaagtact
     4801 aatgtaatta atattttttt cataccagtc tccattttcc gtatcctatt ttcatttaaa
     4861 attcaaatga agatagaata ctctttgtct tcaaatatta acaaaaataa tttacttatc
     4921 tatatgtatt taagtgacat aaaatagaat cctagaacat gcaggtaata acccacgtgt
     4981 tctaggattt ttcttacttt ttatttaaca gaccaatagg attcatctac accagctgta
     5041 tcaggtgcac ttgcatatga agtgtaacct gggttcgtcc atggagaact ataataatat
     5101 tgtccgatta ctgaagattt gtgaactttg cttccgtggt aataatttga ccaaactgaa
     5161 ccaccaccag ttccataatt ccatgttccc ccccctacat attgtgttgc agcaaatgct
     5221 gctgttgctc ctgaaccaag tattgcagca cttaatacta atgaacttac agtttttttc
     5281 aatttcaatg ttttttcccc ctttaacatc taattaattt atttagatat tgcagactga
     5341 ctaccttttt atttttaaac aaccgcatta aaaaataaat atatagaaca gaatacaata
     5401 aactaacaaa taggaagtaa ataaggtaag gatactcaaa tgaaaaaata attcaattaa
     5461 aaatagggtg ggaaatatta acttcaaatc gtttttttaa atattatcac agtttttttc
     5521 aagaaagaca tattcaaaat tttttaaata aaattcagaa ttttctgtaa ttatttgata
     5581 taaaaatagt gatacttaaa acgaaaattt tcaatcaagt aagtatttac aaatatagag
     5641 gggagtaatt ggaaagtatt ttaaatgatg caatttaaga aatagacatt ttcaattttt
     5701 tgttaccatg taatcgtatg gtatgcaatc taaaaaaaat ttgtggtaag tgtgagaaat
     5761 cactttgttt aatacgttat ataaggtata agaaattgtc tatccattat taacgttaca
     5821 tattacaaat gaaatatttc tataatatgt aaataataaa tttttactaa atacgcctta
     5881 gattcacttt cttaattgca taaaaaatta aaataagaaa gaatttagga ggtgggatag
     5941 tatgaaagat atttatgaac tattacaaga gatccatata aacctaactg aatttgaaga
     6001 aagaaaatgt agtgaggtag aaaaagagaa agtaaaaggt tttgtaaaag agttaattaa
     6061 aaagaaggaa gactgaaaat gtttataacg caagtatcaa tctaaagaat gaagcgataa
     6121 ggcttctaac cgcaaggact gtggagatag cctcctaact ctttacttag gaatccccct
     6181 cttctaaaag aagtgaaagt gggggtagtt taatcataag gagtcattga cgtatttgaa
     6241 acatgaaaat taattgtatt aactttaaat aacagcacgg tattagtaca tccattgacc
     6301 tttaattttt acatatgtct catcaatccg ctaagaatcc ttgattgatg tagggggttc
     6361 tggaacaaag attattattt tttacttaca attagtagat ttgtaatcta gtagtatttc
     6421 aacaatggaa ttaatagttt tgttttgggc actatagtag acttctaatc cttttctatc
     6481 atttctaatt aatttttgag atttcatctt tgctaaatgc tgtgaaacag tagattgtgg
     6541 gatacccagt atacctacaa tttgtgttac attacatgcc cctcttataa ttaattcttt
     6601 taaaatttca attcttactg gatgagctaa tgccttaaaa acttctgcgt ctttttcata
     6661 atttaacaaa ctaaacatct ccttttgaat cgatatatgt ttataatatt aataatttat
     6721 tctgcatcat ctttttgatt taattagttt tttatttttt caacataccc tggtatgctt
     6781 gagacactct tattctcttt tttttacttc ctttaattca tctgaaaatg ttgcatttgg
     6841 ctttttcata taatacataa ttctaaaatc atatttgatg gtttctaacc atatgcttgt
     6901 acttaggtat cttgtcttaa ccaaatcagc gatttctagt agcagaaatt gacctttacc
     6961 aaggaattga ttaccttcat gtaatttcgc aagaatcttt tttgggctag gtcagaaatt
     7021 ccaatagtta atagcgattg tgtaaatcct aaacctaatg tatcgacttg tttgatacat
     7081 attaactatt caccagaacc acatgtaact aatttttttc aaagaatatt tttctataaa
     7141 aaactgcaac tctacttgtt agttatgtct aacaagtaga gttgcagttc accaattagg
     7201 ttatagttta cttgctattt agacacgtac agctgtaccg cttactgcaa ccattagcat
     7261 tccatcacga acgacttcgt aatctacatc gataccaaca attgcatttg ctcctttttg
     7321 cgttgcaagt tgtttcattt cttccatggc aatgtcacga gcttctttta atttactttc
     7381 ataagcacca gaacggccac ctacaacgtc acgaacagaa gcaaagatgt cgcgtacgat
     7441 atttgcaccc ataattgctt caccgtttac gatgtctata tactcaataa tttctttacc
     7501 ctgaatagta ttagttgttg ttacgatcat tattaacaca ctcctataat ttttaaaaat
     7561 ttattgttga ttgctataat cctattatta attttaaaag ctaaataaac tatctaatat
     7621 ccttacataa agatgacagt tctgtaaggg tgaaagaaaa caagttatcc acaaaaaact
     7681 tgttttctta acactaatat taaaatgctt tcaatagtaa aaatagtttt tgtaagtttt
     7741 gtaaatactt ttgtaattaa aaaaaagtat aagggaatcg attttctgcc aaattagttg
     7801 caatgtttat tttcgttcca tctatatcct tttcctcgta ccgtaaggag aaactcagga
     7861 ttagacggac tagtttcgat cttctgacga agacgattga tattcacatc gatgactttt
     7921 gtattaccta cacaatttat accccatatt ttatttaaga gttcattacg tgaaattggt
     7981 ttcatggaat tttccattaa atatcgtaaa ataatatgct ctgttggagt taaatcaatt
     8041 actatctcat tcctatataa ttctcctagc agaagattta attgaaatgg ccccgaatga
     8101 atcaattcca aatccttttt atttttttga attcttctta gcaatgattg tacacgtgct
     8161 actaattcaa ttatactaaa tggcttttta atataatcat ctgccccaat tgtcaaaccg
     8221 tgtacctggt ctttctcttg aacaagtgct gttaatataa taatacctat atcttcattg
     8281 ttttttcgta tgcttttgca tacccgaaat ccatctattc ctggtaacat aacatcaagt
     8341 attacaatat caacattatg ctgttctaaa agctcaagag ctttttcacc tgtatcggtt
     8401 tctaaaacat tgaaaccagc acgcttcata ttcaatgtaa taaaactacg gatggttata
     8461 tcatcttcta atactaaaat tgttgtcata ctcaccgctc ctttactctt gttgataaac
     8521 ggctattggt ttgatataag ttctaagatt ctggtagttt atttttaaaa aattgtatat
     8581 acatgagctt ttttttatat gtatcaactc ctaggagtca ctatatatgt ccatcaactt
     8641 aaaaatctag taaaaaactg tcatcctttc tatttagcct ttttgaaaga ataacgtgga
     8701 gctagtaaag attcttaagt tggtggacat ggagtggtat ccctagttta tttgccttat
     8761 attaattaag tgataggact ttttcgtcct taatctcatt ctcctgtcaa ctctaccacc
     8821 acataattta ttacgtgaaa acgaaatatt tttcatcgtt tgctcttaaa tttctacgtt
     8881 tttatataca cctcaaaacg ctttgactag ataatccaag ttctcttcgg attcacctaa
     8941 ataatgttga gcctcttgaa actccccaat cgattagctg attgatgatg caccattttt
     9001 ttctttctac atgatagatg tgctgttcta gtttttttgg atcttttgca tgctttcaag
     9061 aaatcaattc ttgtgttgcc atttctacaa actgaatatc agactctaca aggtattgaa
     9121 tatatgccgt tttcgccttc atatattcat cttcattttt ccaactgtga tggctgtcgt
     9181 cgtaaagtat aaaaaaggag tcgcttttca acggacttat caatttattc atttcattat
     9241 tcggattaaa tatttctttt gcttttttat gtgcctgtaa cactttgtct ttcgaacatc
     9301 cgcctacaag caaataatgt ggagtgatta aactcatttt gttatttccc cccctctatt
     9361 gccctcatat acgatccaga aaggatagga acaaagagca cgcattgatt ctttacaagc
     9421 taacataatt taaaaagcat gagaggaagg gtaatctcaa atggctagtg atatattcta
     9481 aaaagctgtc cttcttccca ctttgtacaa caaacacctt aagtttgaat aaatgagctt
     9541 gttctttata cttagcttaa atataaagga tagttccaat taaaaatgat tatgtagttc
     9601 tcccccattc gaatccaacg gtttcatgaa gcttttgttt tgtcatttcc attgtgaatt
     9661 cttgggcctg ctttttcgaa tggatatcta caattccatt cgaagcaatc attttttcac
     9721 cctttcgtag ttatggcgtt ttcatataac taaatacaat acaactaacc aaaatggcat
     9781 taccattaaa aatccatttc taatccctat tgcaaaagcc aacggatcaa cattacagta
     9841 tgacgttgcc tcttttttaa ctcgctgcaa catctttttt tgggtgggag ggctcatagt
     9901 gaatctcctt atctttcaat ctattttcgt tttccataac aaactaccac caaaacagcc
     9961 tatacaataa atcacaatga atttcataaa aatttctata attgcatccg tcattcccta
    10021 ttctcctcgc ccaaggcctg taatacacaa gctataatta gcaaaataag aattaataat
    10081 aagctcccta tatttcggca ttttctaagc atcggcattc tctccttata attaacgttt
    10141 attttagaaa aaaaccaagc taagagtatt aagagaagaa aagagcccaa tttacctcgt
    10201 tatgagcaaa tcggctcttt aatattcaaa tgacataagc aatgtaaact tattgggtgt
    10261 aatctcccaa acttatattc tcctaaga
//
