LOCUS       NZ_KB976667            19402 bp    DNA     linear   CON 01-APR-2024
DEFINITION  Bacillus toyonensis strain HuA2-3 acrHg-supercont1.5, whole genome
            shotgun sequence.
ACCESSION   NZ_KB976667
VERSION     NZ_KB976667.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus toyonensis
  ORGANISM  Bacillus toyonensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 52057)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C.,
            Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T.,
            Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     The Genome Sequence of Bacillus cereus HuA2-3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 52057)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C.,
            Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T.,
            Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2012) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Taxonomic-Update-Statistics-START##
            This Genome (query)               :: GCA_000399405.1
            Current Name                      :: Bacillus toyonensis
            Previous Name                     :: Bacillus cereus HuA2-3
            Date Updated                      :: 2022-03-10
            Analysis Type :: Average Nucleotide Identity (ANI)
            Analysis 1 (A1) :: Query vs. TYPE genome for current name
            A1 Genome (subject)               :: GCA_000496285.1
            A1 Name                           :: Bacillus toyonensis
            A1 ANI                            :: 99.4201%
            A1 Query Coverage                 :: 83.75%
            A1 Subject Coverage               :: 94.68%
            Analysis 2 (A2) :: Query vs. TYPE genome for previous name
            A2 Genome (subject)               :: GCA_006094295.1
            A2 Name                           :: Bacillus cereus
            A2 ANI                            :: 91.7421%
            A2 Query Coverage                 :: 77.41%
            A2 Subject Coverage               :: 80.96%
            ##Taxonomic-Update-Statistics-END##
            ##Genome-Assembly-Data-START##
            Assembly Method                   :: allpaths v. R41865
            Assembly Name                     :: Baci_cere_HuA2-3_V1
            Genome Coverage                   :: 108.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000399405.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 01:17:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,841
            CDSs (total)                      :: 5,738
            Genes (coding)                    :: 5,542
            CDSs (with protein)               :: 5,542
            Genes (RNA)                       :: 103
            rRNAs                             :: 6, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 3, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 81 of 196
            Pseudo Genes (incomplete)         :: 115 of 196
            Pseudo Genes (internal stop)      :: 62 of 196
            Pseudo Genes (multiple problems)  :: 52 of 196
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:43:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 19402
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB976667.1.
            Please be aware that the annotation is done automatically with
            little or no manual curation.
            The organism was changed from 'Bacillus cereus HuA2-3' to 'Bacillus
            toyonensis' in March 2022.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..19402
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..19402
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..19402
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[6573:9402](+)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      6574..9402
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     gene            175..2280
                     /locus_tag="IG5_RS06145"
                     /old_locus_tag="IG5_05720"
     CDS             175..2280
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592623.1"
                     /locus_tag="IG5_RS06145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05720"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_000592626.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.5e-34, bitscore:
                     109.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKKRVKFIPQMTSYDCGPSCLAMILHYYGLSIKPSDIRKNKIVN
                     KNTAWSLLDIKKVSDSYGFSTNAYRIDNINDLQMINKPVIAFWGLNHFVIIEKVKKNRF
                     YIVDPKAGPMIIDEKSFKEMFCNYILLIESTADVKVSNKSNEKYLLFKYMHHFLSLNQI
                     IMLGLLSLLAQISLFSFPFILKELMDILDTSKELNIKYFLIIISGLVVAIGIKSLSSIV
                     TIRFQKDIDRKGTTKVFEHIFNIPIENITTRHVGDLNVRILGLEEIRGYILEDLIQMLV
                     SIVVIIPMLIYLLYSEPLYTGILIFFILCSLFINISLGKSLFNSHLVEKHYLGEHQGKI
                     NESLKAMYYVKATGVFSRIKNVWQKDYDKYLDIVHKRTLKQHALESFNGSLSTIFIIIL
                     LAIGFYDYSRGQGDLTKIFFFIAISSIIFSPVTTIIGSVLNWNAIKPLLLRILDVLDED
                     SENDGLNTEKKSITGAIQFNNISFSFNNKLILSDLNLDINKHESIAITGKSGSGKSTLA
                     NLLLKIHTPDKGDILIDGQDIKEWKNDELRKNIGLMTQDGVLFRGSLSENLAYFDIGYD
                     PLKIQDSIQKLNLNNYLQFQSINDIAISEGGTNLSGGQRQRLAMLRLFMKEYPILILDE
                     PTNHLDRQTADTIIENLFHIPSTKIIITHDEKILERVDKVYELTERHLEQRDTDSKYKH
                     DMTKSYI"
     gene            complement(2974..3102)
                     /locus_tag="IG5_RS31720"
                     /old_locus_tag="IG5_05721"
     CDS             complement(2974..3102)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751436.1"
                     /locus_tag="IG5_RS31720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05721"
                     /product="hypothetical protein"
                     /protein_id="WP_000751436.1"
                     /transl_table=11
                     /translation="MKKTKEFLNNPFIKYPLTIISAITILKTGYDLGVFIINVKYI"
     misc_feature    3165..3180
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 24.36"
                     /tool="antismash"
     gene            complement(<3577..4465)
                     /locus_tag="IG5_RS06150"
                     /pseudo=""
     CDS             complement(<3577..4465)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016084124.1"
                     /locus_tag="IG5_RS06150"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTTKETQNTIQGFSSYLSNKGRKPSTIKRYIYDVQSFIQWLHQSK
                     RFTNDNVWESLQKKDFEVFFDYLKAERQYFEKTVHRIYIVLNRLYEYLDLPSPIEGVIH
                     IDPPNRALRREDFIFSQEEKRLKQIVASFEGLTEK"
     gene            complement(4476..5081)
                     /locus_tag="IG5_RS06155"
                     /old_locus_tag="IG5_05723"
     CDS             complement(4476..5081)
                     /GO_function="GO:0000150 - DNA strand exchange activity
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102878.1"
                     /locus_tag="IG5_RS06155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05723"
                     /product="recombinase family protein"
                     /protein_id="WP_001097386.1"
                     /transl_table=11
                     /translation="MNTRKFGYIRVSSKEQNEDRQLDAMKQLITDERDIFVDKQSGRDF
                     NREQYQLVKRMLRKDDILYIHSLDRFGRNKEAILQEWKDITQNIQAHIVVLDMPLLDTT
                     QYKDSLGNLITDLVLQILSWLAEEERTKIKTRQREGIEAAKKKGKHLGRPKTEITQEFI
                     DAYNDWKDGKVTALYAMKKCNMTSPTFYRVVKRYEDKE"
     misc_feature    complement(5266..5280)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 23.01"
                     /tool="antismash"
     misc_feature    complement(5410..5424)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 20.21"
                     /tool="antismash"
     gene            5432..6032
                     /locus_tag="IG5_RS29005"
                     /pseudo=""
     CDS             5432..6032
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016119171.1"
                     /locus_tag="IG5_RS29005"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="IS6 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MILLTVRGYLRYNLSFRDLVEMMEERGLSISHTTIMRWVHQYGPK
                     LDKRIRRYLKQINDSWRVDETYIKVTGQWMYLYRAVDSNGNTIDFCLSKTRDHKAAKRF
                     FKKALRSFHVSKLRMITVDKNSAYPIVLE"
     gene            6315..6482
                     /locus_tag="IG5_RS06165"
                     /old_locus_tag="IG5_05725"
     CDS             6315..6482
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090584.1"
                     /locus_tag="IG5_RS06165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05725"
                     /product="type A2 lanthipeptide"
                     /protein_id="WP_000447118.1"
                     /sec_met_domain="L_biotic_A (E-value: 5.6e-14, bitscore:
                     43.7, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METEKYLQVVEDEELEQLVGGAGPGWVETLTKDCPWKQPGACVII
                     MGQKICKKCY"
     CDS_motif       6315..6377
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: METEKYLQVVEDEELEQLVGG"
                     /locus_tag="IG5_RS06165_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       6378..6482
                     /prepeptide="core"
                     /locus_tag="IG5_RS06165_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="2.90"
                     /molecular_weight="3717.5"
                     /monoisotopic_mass="3714.9"
                     /core_sequence="AGPGWVETLTKDCPWKQPGACVIIMGQKICKKCY"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="METEKYLQVVEDEELEQLVGG"
                     /leader_location="[6314:6377](+)"
     gene            6574..9402
                     /gene="lanM"
                     /locus_tag="IG5_RS06170"
                     /old_locus_tag="IG5_05726"
     CDS             6574..9402
                     /GO_process="GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lanM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794834.1"
                     /locus_tag="IG5_RS06170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05726"
                     /product="type 2 lanthipeptide synthetase LanM"
                     /protein_id="WP_016090759.1"
                     /sec_met_domain="DUF4135 (E-value: 1.7e-78, bitscore:
                     256.1, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 1e-37, bitscore:
                     121.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNNSYGILNTIDNGSHSLVTKEYLEKNFVIFLEDFSCNDVIKND
                     ESYNFQNIIQTYLVFFENMIINKIDFFGNFERVSKSIMFDLEKRLYQVLRPSLIVYINK
                     LRVEKALIGIDTNEEYKYFTNNFFHFKKFHMNFFRMLPLLSEKIFTIIENEINYIIWLQ
                     GALEKDQKEIHKMSGFNIMHVEKVINLGDYHNNGRRTILLEDKFNNKVILKPVDLTNSI
                     LYHSLINQLNKDLNTDIFIPQVVNKNNYGYMEYINHESCESNEQIKGYYYNLGVVLSVI
                     YLINGSDIHNENIIAKQTSPVIIDFETLGSTLNPSITEKTSSFKLSNSVLNSRMLPIRF
                     SEGREVIRDYSAIGRVMKTLVKKIKIKNEFTSNPIEIREETIVEDTVQNLPFFYDDIYE
                     YDNYIKDIIKGFEDAYNGALKNKEEYMHILKSSVSKWNYRKVYRNTKIYTLLLDRLNVP
                     DLLENKLKTIKYLRNILEKNTYLATRKDIIQKEIEALLSYDVPYFSNKYEWESCETFSN
                     NEIDLEMKFEKISYADFLLQRKIIEMSLSNQNDNLDQLNTVKETINFQTNLSTNKIKEA
                     LTGKILDGSYISKDGDVDFIGLKTNWQGELEINHLNNGIYDGLLGISSLLRNNADPNHI
                     EIINKLEQKALQEILNKNKNNYGFVNGSNAIISYYLTNLPYITPLNEKVILQIFMDINS
                     DIEKDEYKDNTFDILGGSAGLILTAVKFYQINPKYKILLKVAENLGDYIVNNMQTHNEF
                     VYWKAHDTLNLEDSLKGFVHGLSGQLLAFYKLKDILKTNKYDRVIHCIHKSEKMSMNEY
                     NYTDSWCKGFSGMGISRIKILELYQNEDIQSDLIFFKNQILSTLYENSDYCLCHGLMGK
                     LDFLLELENRGMLERNEKQIVMKVTNNFLNNFAIEDFNPHKIALFTGLGSILYFLQRLE
                     NDKLNSILYFNA"
     gene            9417..11519
                     /locus_tag="IG5_RS06175"
                     /old_locus_tag="IG5_05727"
     CDS             9417..11519
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944706.1"
                     /locus_tag="IG5_RS06175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05727"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_016090760.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.8e-33, bitscore:
                     107.0, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRKKIKFIPQMTSFDCGPSCLAMIMHYYGLAVQASEIRNSKIIN
                     KKSAWSLLDIKKVSESYELSATAYRIENISDLQRIQKPMIAFWGFNHFVIIERVKENKF
                     YIVDPKAGPMVIDIRSFEEMFCNYILVMEKKENIQPNVKRNEKHILLRYIDHFVSPSQI
                     ILLALLALLAQISFFAFPFIFKNFMDILNASKQVDVRYLLIILGSLIVVIGIKSITTII
                     KVKFQKEIDRKSTTKVFEHMFNIPIEKITTRHVGDLNVRIMSLESIRGYILDDLIEMII
                     SIVVIIPLLMYLLYTETFYTLILIFFIASSLFINIFLGKFIYNANLVENYYLGEHRGKI
                     NESLKAMYYVKATGIFSRIKSVWQKDYNKYLDTVHKRALKQNALESFNGSLSTIFIIIM
                     LAVGFYNFSKGEGNLSNIFFFIAIASIIFSPVSTIIGSILNWNSIKPLLLRTLDILDED
                     LEKDGSDTKVNILRGSIQFNNISFSFDDKKIIENLSLNIKNNESIAIVGESGSGKSTLA
                     NLLLKMHTPDKGNIFIDDRDIKYWNSEELRKKIGLMTQDGVLFRGNLSENLSYFDINYD
                     PSKIQDSIDKLNLSNYIQFQSINDIFISEGGTNLSGGQRQRLAMLRLFMKEYPILILDE
                     PTNHLDRETANTVIENIFAIPSTKIIITHDENILERVDRVYELTDKKLEQREISSKSRL
                     QTLGKG"
     gene            11591..12496
                     /locus_tag="IG5_RS06180"
                     /old_locus_tag="IG5_05728"
     CDS             11591..12496
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761424.1"
                     /locus_tag="IG5_RS06180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05728"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000761416.1"
                     /transl_table=11
                     /translation="MKLAIQAKNLTKSFENQSIVRDLNLSVPEGSLYAFIGPNGAGKTT
                     TMRMLLGLIPLDKGTILFNNKDIKEWKEELFENVGCFIDSPTYYPNLTAYENLAYVQKM
                     INKPLDEIDRVLKTTGIYKAKDKKVKDFSLGMKQRLGISFAILNNPKILILDEPTNGLD
                     PEGVHEIRRLLLHLVKNEGKTIFISSHNLAEIEMIADHIALLHKGSLLYEGNLNSIITS
                     GEYIVHVNDTAATERILNTEGIPYSIEKEGVFKIIVDQDSVPNLTRIIIKNDIDIFEVS
                     PKKPTLEEVFLSLTKQKELL"
     gene            12499..13224
                     /locus_tag="IG5_RS06185"
                     /old_locus_tag="IG5_05729"
     CDS             12499..13224
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016093811.1"
                     /locus_tag="IG5_RS06185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05729"
                     /product="ABC transporter permease"
                     /protein_id="WP_001159645.1"
                     /transl_table=11
                     /translation="MQFIFKSVNSEMRKIGGRKLLLISLFFSLLSSFLCISYFIFNKNN
                     PDMFQSTNLIAITVNFNTLTLSIFSACMWYFIISSENKYGTWAIIYTKPISKFKLLVAK
                     NLLFILFYLIFNILNFSVMGIYTFINGYEISILYFLKMLILCTILSLAIPYSQLLFHLI
                     IKKGILAASLSVIWIFFIVTYGLFPLIFTKTFPIFYALNIVSDVMFKETTILLYLASSS
                     IYILGIMLLTVSFKYYTYE"
     misc_feature    complement(12510..12525)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 23.71"
                     /tool="antismash"
     gene            13240..13938
                     /locus_tag="IG5_RS06190"
                     /old_locus_tag="IG5_05730"
     CDS             13240..13938
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511800.1"
                     /locus_tag="IG5_RS06190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05730"
                     /product="ABC transporter permease"
                     /protein_id="WP_000511809.1"
                     /transl_table=11
                     /translation="MGILGEYKKSLHNGLYILLLVFLLVPISFQVFLTVSPQLNDGAFM
                     HIAILSLFCRLFLPIAIGLLVSFSFVNEFRRKGYMSAIYHGITLKTILSNKFFFCLIFT
                     FCYLICALIITSIPIVLTVDNLGTVYQSLSFSILFLVISSLIIVNLHFLINIIFYKIPL
                     LSLLVGVIGGLGSFFISHTNYWILFPWSYPIMSLYFDSIEPKQLYIILIVYLLSLLGCI
                     FIIKKSIRWN"
     gene            complement(14493..14684)
                     /locus_tag="IG5_RS06195"
                     /old_locus_tag="IG5_05731"
     CDS             complement(14493..14684)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367313.1"
                     /locus_tag="IG5_RS06195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05731"
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="WP_000367310.1"
                     /transl_table=11
                     /translation="MDMKLLHEMIEDKKKELEYLVKTYGFRHQKVISVSQKLDLLIYKA
                     MNRYRLDYKIRTKKESLY"
     gene            complement(14894..15082)
                     /gene="hfq"
                     /locus_tag="IG5_RS06200"
                     /old_locus_tag="IG5_05732"
     CDS             complement(14894..15082)
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_process="GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /GO_process="GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="hfq"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990401.1"
                     /locus_tag="IG5_RS06200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05732"
                     /product="RNA chaperone Hfq"
                     /protein_id="WP_000990397.1"
                     /transl_table=11
                     /translation="MMYTQEKFYEKLIEEKKEVTVFLINGVRVPGKIIAVDKFSVLILS
                     NGKQMFLYKQAISTVSL"
     gene            complement(15079..15936)
                     /locus_tag="IG5_RS06205"
                     /old_locus_tag="IG5_05733"
     CDS             complement(15079..15936)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055172.1"
                     /locus_tag="IG5_RS06205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05733"
                     /product="hypothetical protein"
                     /protein_id="WP_000055172.1"
                     /transl_table=11
                     /translation="MSLLQQHFEARREYIFNRLKQPEYMERSIEKVRQAQKEIKNTVRT
                     IKDLLLLDKTTDPCLPEVAQFSLQHIMNSESFENVKKLVPSSIKKLSEEERAKVLDETL
                     SVANQIMNLERTVFIMMFNTKEKILMDAYKKKPRSQTELHYDVADKEGFDKAFYEERID
                     SLQNDIRVLSFKKLCENEPAPEDLELFKQRYETIILPKVQEIVSLIEPSLIDVDVFLNS
                     VIEYGVGEITLDEMIQKLHKNLSLFHELSKVEYCPTVELTVKEYVFLEAMNRSEKGEEL
                     QPSK"
     gene            16239..16673
                     /locus_tag="IG5_RS06210"
                     /old_locus_tag="IG5_05734"
                     /pseudo=""
     CDS             16239..16673
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016123544.1"
                     /locus_tag="IG5_RS06210"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05734"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MNNSYEIIGNQIKYYLRDDPTRG"
     gene            16667..16990
                     /locus_tag="IG5_RS06215"
                     /old_locus_tag="IG5_05735"
     CDS             16667..16990
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934651.1"
                     /locus_tag="IG5_RS06215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05735"
                     /product="hypothetical protein"
                     /protein_id="WP_000934652.1"
                     /transl_table=11
                     /translation="MLNRKETISFQAFMDQSYKKKKQTKIFSVGPISPFAFFHMSSPLV
                     HTYVALGIVGGLTIGAVLLEKYLVRNDYVFASNILSNGLYYGVRIGGIGFIGYVFIRIL
                     LML"
     gene            17003..18166
                     /locus_tag="IG5_RS06220"
                     /old_locus_tag="IG5_05736"
     CDS             17003..18166
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679433.1"
                     /locus_tag="IG5_RS06220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05736"
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="WP_016090762.1"
                     /transl_table=11
                     /translation="MKGWLKKKRYKYQLEEVFKKAKLYDEHVTRGGKLPIYPKIHDVYA
                     GKESVRYTFTLPNGVDPAQIHKKWFCFQQILGENLMMEGNIKKFVLHVFHSNASLQPYD
                     YSYKQWQPLLKQYRLPIVVGRDQFGNTIVYDMVDSNTPHLLIAGETGSGKSSIVRVILA
                     TLIQQMSPEHLHLYLGDLKNSEFHFLRRVKHVKYVCMEEHEMTNMLAKLWKEVLHRRKV
                     MEKYELGHIDEYNQITKDKPLPYIFIAIDEVAMLQDEKECVTIIEKISAVGRSLGIFLM
                     LSMQRPDAKILDGKLKLNLTVRMGFKCADLINSNIVGTPGSENLSQSGQMILKLDGLKK
                     VQAPYLALDQAKEIIEPYRLSKEQSIKKEEPEHQEDKIFGVLDDANE"
     gene            18150..18803
                     /locus_tag="IG5_RS06225"
                     /old_locus_tag="IG5_05737"
     CDS             18150..18803
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002105922.1"
                     /locus_tag="IG5_RS06225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05737"
                     /product="replication-relaxation family protein"
                     /protein_id="WP_002105922.1"
                     /transl_table=11
                     /translation="MMLTNRDKQIIGHLQQFRCMSRDDIIDLHFRNVKNAVTCCNTVTK
                     RLSREGYIDVDTSQRPYVYFPYPNTIRKGSQKIRHFLAIVDVYKQLLRTERPKIFQVEP
                     KYKKGYMEPDIFTIWHKSPFFIEVQNSIYNKATIQTKIKRYESYFRSMEWKKESWQPKH
                     NMVFPSLLILTDLKYPISSSNFRIFQAPSIQHFLIQVQKPKDISVQIGSVKAKL"
     gene            complement(18832..19053)
                     /locus_tag="IG5_RS06230"
                     /old_locus_tag="IG5_05738"
     CDS             complement(18832..19053)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466110.1"
                     /locus_tag="IG5_RS06230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IG5_05738"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000466112.1"
                     /transl_table=11
                     /translation="MFFRKNRTKLKRWLDRQGIEQQELSKKSKVSVQTISKACRDTDYI
                     PKPEIMKKILNTIRKIDPHAKINDFWDM"
ORIGIN
        1 taatgaaaaa gaaatcttta tacacgcagt aaataatttt actatagaga attttaatcc
       61 gtataaaacc tcattattta caggtttaag cggtatatta tattttcttc aaagattaga
      121 aaatagtaag atgaatagta ttttatactt taattactaa aacaggtgat acatatgata
      181 aaaaagaggg ttaaatttat tcctcaaatg acatcttatg attgtggccc tagttgcctt
      241 gcaatgattt tgcattatta tggattatcc attaagcctt cagacattag aaaaaataaa
      301 atagtgaata aaaacacagc atggagtttg ttagatatta aaaaggttag tgattcgtat
      361 ggtttttcaa cgaatgctta cagaatagat aatattaatg atttacaaat gataaataaa
      421 ccagtaatag cattttgggg gctaaatcat ttcgttataa ttgaaaaagt taagaagaat
      481 aggttctata tcgttgatcc taaggctggt cctatgatta ttgatgaaaa gagttttaaa
      541 gagatgtttt gtaactacat cttacttatt gaaagtacag cagatgtaaa agtaagtaat
      601 aaatcgaatg aaaaatatct tctatttaaa tacatgcatc attttttatc acttaatcaa
      661 attattatgt tggggttact atccttatta gcacaaattt ctttattttc ttttccattc
      721 attctaaaag aacttatgga tatattggac acctcaaaag aattgaatat aaaatacttt
      781 ttaattataa taagtggttt agtagttgct ataggaatta aatctttgag ttcaattgtt
      841 acgattaggt ttcaaaaaga tattgatcga aagggtacaa caaaggtttt tgaacatata
      901 tttaatattc ctatagagaa tattacaaca cgccatgttg gagatttaaa tgtaagaatt
      961 ttgggtttgg aagaaattag gggttatatt ctggaagatc taattcaaat gttagtatca
     1021 atagtcgtta taatacctat gctcatttat ctcttatata gtgaaccttt atacactgga
     1081 atacttatat tctttatcct ctgctctcta tttataaata tttctttagg aaaatctcta
     1141 tttaattctc atttagtaga aaagcattac ttaggagaac atcaagggaa aataaatgaa
     1201 tctttaaagg caatgtatta tgttaaggca acaggggttt tttcaagaat taaaaatgta
     1261 tggcaaaaag attatgataa atatttagat atagtacata agcgaacttt aaaacaacac
     1321 gctttagagt catttaatgg ttccctatcc acaatattca ttatcatact gcttgctata
     1381 ggtttctatg attatagtag aggacaaggg gatttaacca aaatattctt ttttattgct
     1441 atttcaagta ttatattctc acccgttaca accattatcg gatcagtttt gaattggaat
     1501 gcaattaaac ctctattatt aagaatttta gatgtattag atgaagattc agaaaatgat
     1561 gggttaaaca ccgagaaaaa atccataaca ggtgcaattc aatttaataa tatctctttt
     1621 agctttaata ataaacttat tttaagcgat ttaaatttag atattaataa gcatgaaagt
     1681 attgcaatca ctggaaaaag tggtagtggt aaatctactt tagctaattt gctattaaaa
     1741 atacatactc cagataaagg ggatatttta atagatggac aagatattaa agaatggaaa
     1801 aatgacgaat taagaaaaaa tatcggatta atgactcaag atggcgttct ctttcgaggg
     1861 agtctttcgg aaaatttagc ttattttgac attggatatg atcctttgaa aatacaagat
     1921 tctattcaaa aattaaatct gaataattat cttcaatttc aaagtataaa cgatattgct
     1981 atatccgaag gaggaacaaa tttatcgggt ggacaaagac aaagattagc tatgttacgt
     2041 ttattcatga aagaataccc tattttaatt ttagatgaac ctactaatca tttagatagg
     2101 caaacagcag atactataat tgagaattta tttcatattc cttcaactaa aattattatt
     2161 actcatgatg agaaaatttt ggaacgtgta gacaaggttt atgaacttac tgaaagacat
     2221 ttggagcaaa gagatacaga ttcaaaatat aaacatgata tgactaaatc atatatttag
     2281 caaaaacata ataagttttg attattttta cataatttca aaaaaatgat atttgtatta
     2341 atttaatcgt ggtatactaa ttttataaga aggagggata ccatgccata tccaggagta
     2401 attaaagcag tttggaagta tatatttaat agatagaatt tgattttaaa ataacccgaa
     2461 aaagggctat tttaaatttg tttttttaca aatctaccca tctcttaagg gaggggattt
     2521 cccgcccaga gaatctgttg tggatttgaa ataaagtttg atcaaaaaca tcatacaaac
     2581 tacattctat ggtaaataaa aagaatccat tttgaaaaaa gattgaacaa aatggattct
     2641 ttctttgtga ttgggtgttt agtttttata aacagtccca ctttatttta aaccaatagc
     2701 atattaaaca attccctcag gaatgatata ttcatcacaa aagtaattat gatattgagc
     2761 tatccattca tatttagagt agagcttact atcattctgg tgcaaaatat ttgaagtata
     2821 tgaaaatggt ctatcaaatt ttattccaaa ccaacaccag cagcatagcg ttatattgtt
     2881 ggttttttat ttatgtattt agtgtagacc tagattttaa gtcgcgatga ttctatgaaa
     2941 attgtgatat tttgtcgctt tcattatgat gatttaaata tatttcacat tgataataaa
     3001 gacacctaaa tcataacctg tttttaatat agttatagct gaaattatag ttaacggata
     3061 tttaataaat ggattattta ggaattcttt tgtttttttc atgttgtaat cctccagtaa
     3121 ttgataatct tagtttaaat attatcatga atatgcatat aatgattgat aattattata
     3181 tttaactttg atcaaatata atgtgaaaaa aggtaattta aataaggttg gtatagcgct
     3241 atgttaattt tggcttgatt ctatttttat catttgaact tgaaagatat cgcagtcctt
     3301 tattcccagg acacgcattt cttctttaaa tgaatgctag gattcggttt taatagaatt
     3361 agtagtttta tctagtagcg tatactcatg tacgtaatat gatttttcat tttcttcatt
     3421 gtattttctc caatcctcct taaaataatt gaaaaactgt agttatataa aaattacata
     3481 ttaaatgatg gtttttaatc tgtttttggt tggtgtttcc tataataaat aaatcgtaat
     3541 atctttttaa aaatattacg atttaaagct gatttactgc atgatttgtt cagaagtgtt
     3601 tttctgttgg atcattctaa atataaagtt gtttctgaaa tgttgggcag aaattccctt
     3661 tcgaagattg gcacatttta cttctaaacg gatcattttt tgtacagaga tttcagttaa
     3721 atattttggt gcatcatcgt catagctcca atggtatgtt ctacgagtaa agtcaaaagc
     3781 aacaaataat ggattgctgc tgtgataatt aggacgaaca ggatcaggga tggttttata
     3841 ataatcatat aaatgtaatt tgtcttcttc tttcaaatgg atgatacgat ttatttttga
     3901 gtcttccgga atagatagcg tattgttttc gaaatgaacg tgtctcattt gtaaagagac
     3961 acgttcattt cgaaaacaat acgtagtcta ggagtaatat aatgatagaa acattttgtt
     4021 ttaagagcat ggggcgtatg gattgttatt tttctgtcag tccctcaaat gaagcaacta
     4081 tttgttttag acgtttctct tcttgggaaa aaatgaaatc ttctctacgc aatgctcgat
     4141 taggtggatc tatgtgtata acgccttcaa tcggacttgg taaatctaag tattcgtata
     4201 atctgtttaa aacaatatat atacggtgta cagttttttc gaagtattga cgttcggctt
     4261 ttaaatagtc aaaaaatact tcgaaatctt ttttctgtag tgattcccat acattatcgt
     4321 tagtgaacct ttttgattgg tgcagccatt gaatgaaact ttgcacatcg tagatatatc
     4381 gtttgatcgt agaaggcttt cttcctttgt ttgataaata agaagagaag ccttgtattg
     4441 tattttgggt ctcttttgta gtcatactat atttgtcact ccttatcctc ataccttttg
     4501 actactctat aaaatgtagg acttgtcata ttacactttt tcattgcgta tagcgctgtg
     4561 acctttccat ctttccaatc gttatatgca tcgataaatt cctgtgttat ttcagttttg
     4621 ggtctaccca aatgctttcc ttttttcttt gcagcttcaa taccctcacg ttgacgagtc
     4681 tttatttttg ttcgttcttc ttcagcaagc caggacaaga tttgaagtac caaatcagta
     4741 ataagatttc ctaaactatc tttatattga gttgtatcta gaagtggcat gtctaatacg
     4801 acaatatgag cttgtatatt ttgggtaata tctttccatt cctgaaggat agcttcttta
     4861 tttcttccga aacgatctag ggagtgaata tataaaatat catcttttct taacatgcgt
     4921 tttacaagtt ggtattgttc acggttaaag tctcttccgc tttgtttatc gacaaaaata
     4981 tctcgttcgt ccgtgatgag ttgtttcatt gcgtcgagct gtcgatcttc attttgttct
     5041 ttgctactaa cccttatata tccgaatttt cttgtattca aaataaatag acatcccttc
     5101 aaaaaatatt cctgataaaa ttataacaaa aatacttcaa aaggtataat atattattta
     5161 gtacttcaaa aatgtatttt ttaacgtttt gaagcactga atttaataga gtttactatg
     5221 catcaaaagg tgtacctttt gagactacca aaatatgttt tgattttaaa atgagattca
     5281 tgacatattt aaagctgttc tggtacaaaa actttgaaat atatgaaaat agtctatcaa
     5341 acttggatat actgatacta ttactctaaa aaggtgtgtg attggtatgg aaaagaaaaa
     5401 ttagttcaaa tggaagcact atcagcctga tattatctta ttaacggtaa gagggtacct
     5461 acggtacaac ctaagttttc gtgatttggt ggaaatgatg gaggaacgtg gtttatccat
     5521 ttctcataca acgattatgc gttgggttca tcagtatggt cctaaattgg acaaacggat
     5581 ccgacgttac ctcaaacaaa taaatgactc ttggagagtc gatgaaacat atatcaaagt
     5641 aacaggtcaa tggatgtacc tgtatcgtgc tgttgattcg aacggaaaca caatcgattt
     5701 ttgcctaagt aaaacaagag atcataaggc tgcaaagcgc tttttcaaga aagctttgcg
     5761 gtcttttcat gtttcaaagc tacgtatgat aacagtcgat aagaactcag cttatcctat
     5821 agtacttgaa taacatagta aaacaagatc atcgctttat aaagaagcga atctgtccta
     5881 cgctaggtct taaaactttt gacacagcta catccattct ttctggagta gaagccatgc
     5941 atatgattaa aaaagaacag attgctttac ggaaccagtc tgtccaaaat caaaaagaat
     6001 tcatccatca attgtttggg cttgcaaaat aagatacgat tccgttagga attatatgtg
     6061 ctttattctc ttttgttcta tttttgcacc agaaccgaaa atagcaccta aattttaggt
     6121 ggttaaataa tatatagttc tttatttagt atatttatat agtgagttat ctaattattt
     6181 tattattttg gatttttttc taaaaacacc tttttttttt gaatatttaa tgatatatta
     6241 tttctagaca atgttgtggt aaaatattga atttataaca tcattttcaa aaaaataaaa
     6301 aggtagggat tgaaatggaa actgaaaaat atttacaagt tgtagaagat gaagagcttg
     6361 aacaattagt tggtggtgca ggacctggtt gggttgaaac tttaactaaa gattgtcctt
     6421 ggaagcaacc tggagcatgt gtcataatta tgggacaaaa aatttgtaaa aaatgctact
     6481 aatagttaaa gagagtaagg cgaaaggcct tactctcttt aatgataacg aatttctaac
     6541 tatttctcct acctataaaa agaagaggtt attatgaaaa ataattcata tggaattttg
     6601 aatacaatag ataatgggtc acattctttg gtaacaaaag aatacctaga aaaaaatttt
     6661 gttatctttt tagaggattt ttcatgtaat gatgttatta agaatgatga aagctataac
     6721 tttcaaaata taatacaaac atatttagta ttctttgaaa atatgataat aaataaaatt
     6781 gatttttttg gtaatttcga gagggtttcc aaatcgataa tgtttgattt agaaaaaaga
     6841 ctatatcaag tattaaggcc atccttaatt gtatacataa ataaacttag agtagaaaaa
     6901 gctttaatag gaattgatac caatgaagaa tacaagtatt ttacaaataa tttctttcat
     6961 tttaaaaaat ttcatatgaa tttttttaga atgttacctt tattaagtga aaagattttt
     7021 acaataattg aaaatgaaat aaattatata atatggttac aaggagctct tgaaaaagat
     7081 caaaaagaaa ttcataaaat gtcgggtttt aatattatgc atgtagaaaa agttattaac
     7141 ctaggtgatt atcataataa tggaaggaga acaatacttt tagaagataa atttaataat
     7201 aaggtaattt taaaaccagt tgatttgaca aattctattc tatatcatag tttaattaat
     7261 caactgaata aggatttaaa tacagatatc tttattccac aagtagttaa taaaaataat
     7321 tatggatata tggaatatat taatcatgaa tcatgtgaat ctaatgagca aattaaaggt
     7381 tattattata atttaggtgt agtgctttct gtaatatatt tgattaatgg aagtgacata
     7441 cataatgaaa atatcatcgc taaacaaacc tcgccagtca taattgattt tgagacatta
     7501 ggaagtacat taaatccaag tatcactgaa aaaactagta gttttaaatt atctaattca
     7561 gtattaaatt ctagaatgtt acccataaga tttagtgaag gtagagaggt aataagagat
     7621 tacagtgcaa ttgggagagt aatgaaaact ttagtaaaaa aaattaaaat aaaaaatgaa
     7681 tttacatcga acccaattga aattagagaa gagacaatag tagaagatac agttcagaat
     7741 ttaccgtttt tttacgatga tatatatgag tatgataatt atataaaaga tattattaaa
     7801 ggttttgagg atgcgtataa cggagcctta aaaaataaag aagaatacat gcatatattg
     7861 aaaagtagtg tttctaaatg gaattatcga aaggtatatc gaaatacaaa aatctatact
     7921 cttttattag atagactaaa tgttccggat ttattagaga ataaattaaa gactataaaa
     7981 tatttgcgaa atattcttga aaaaaacacc tacttggcta ctaggaaaga tattattcaa
     8041 aaagagatag aggcactatt atcctatgat gttccatatt tctctaataa gtatgaatgg
     8101 gagtcttgcg agactttttc aaataatgaa atagatctag aaatgaaatt cgaaaaaatt
     8161 tcatatgctg actttttatt acaaagaaaa attatagaaa tgtccctttc taatcaaaat
     8221 gataatctag atcagctgaa tacagtaaag gaaacaatta attttcaaac taatttatct
     8281 acaaataaaa taaaggaagc acttacaggg aaaatccttg atggtagtta tatatcaaaa
     8341 gatggagatg tagattttat tggattaaaa acaaattggc aaggtgagct tgagataaac
     8401 catttgaata atggaatata tgatggatta ttgggaataa gttcattatt gagaaataat
     8461 gctgatccta atcacattga aattataaat aaactcgaac aaaaagcctt gcaagaaata
     8521 ctaaacaaaa ataaaaataa ttatggtttt gtaaatggtt ctaatgccat tatttcttac
     8581 tatttaacga atttaccata tataacccct cttaatgaaa aggtgatatt acaaatattt
     8641 atggatatca actcggatat cgaaaaagac gaatataaag ataatacttt tgatatatta
     8701 gggggttcag ctggtctaat tttaactgca gttaagttct atcaaattaa ccctaagtat
     8761 aaaattttgc ttaaagtggc tgaaaatctc ggcgactaca ttgtaaataa tatgcaaaca
     8821 cataatgaat ttgtatattg gaaagctcac gatactctaa atttagaaga ttcattaaag
     8881 gggtttgttc atggactatc aggacaactt ctcgcatttt ataagttaaa agacatatta
     8941 aaaaccaata agtacgatag ggtaatacat tgtatacata aaagtgagaa aatgagtatg
     9001 aatgaatata attatacaga tagttggtgt aaagggttta gtggaatggg aatatcaaga
     9061 attaaaattt tagaattata tcaaaatgaa gatatacaat cagatttaat cttttttaaa
     9121 aatcaaatct taagtacatt atatgaaaat agtgattatt gtttatgtca tggtttaatg
     9181 gggaaactag actttttatt ggagttagaa aatagaggaa tgctagagcg aaatgaaaaa
     9241 caaattgtta tgaaagtaac aaataacttt ttgaataatt ttgctataga agattttaat
     9301 cctcataaaa tcgcattatt tacaggttta ggaagtattt tgtatttttt acaaaggtta
     9361 gagaatgata aactaaatag cattttatac tttaatgcct agaataggtg ataaatatgt
     9421 taagaaagaa aattaagttt ataccgcaaa tgacatcttt tgattgtgga cctagttgtc
     9481 ttgcaatgat tatgcattat tatggtttag cagtacaggc ttcagaaatt agaaatagta
     9541 aaattattaa taaaaaatca gcatggagtt tgttagatat taaaaaagtt agtgaatcgt
     9601 atgaactctc tgcaactgca tataggatag aaaatataag tgatttgcaa aggatacaaa
     9661 agccaatgat agcattttgg gggtttaatc actttgtaat tattgaaagg gttaaagaga
     9721 ataaatttta tattgtagat cctaaggcgg ggccaatggt aatcgatata agaagttttg
     9781 aagagatgtt ctgtaactat atcttagtaa tggaaaagaa agagaacata cagccaaatg
     9841 ttaaaagaaa tgaaaaacat atactactta gatatattga tcattttgta tctcctagtc
     9901 agattatttt actagcacta ttagcattgt tagcacaaat ctcatttttt gctttcccct
     9961 ttatttttaa aaattttatg gatattttga atgcttcaaa gcaagtggat gtaagatatc
    10021 ttttaattat attgggtagt ttaatagtag ttatagggat aaaatctata actactatta
    10081 taaaagtaaa atttcaaaaa gaaattgata gaaaaagtac aacaaaagtc tttgaacata
    10141 tgtttaatat ccccatagaa aagattacta cacgtcatgt aggggattta aatgtaagaa
    10201 ttatgagtct agagtcaata cgaggatata ttttagatga tctaattgaa atgattatat
    10261 caatagtagt tatcattcca ttattgatgt atctattata tacggagact ttttatactt
    10321 taatattgat attttttatt gctagttccc tatttatcaa tattttctta gggaaattta
    10381 tatataatgc taatttagta gaaaattatt acctaggaga acatagaggg aaaattaatg
    10441 aatcattaaa agcaatgtat tatgtaaagg ccacagggat tttttctaga attaaatctg
    10501 tatggcaaaa agattataat aaatatctag atacggtaca taaacgagcg ctaaaacaaa
    10561 atgctttaga atcatttaat ggttccctat cgacaatttt tattatcata atgcttgctg
    10621 taggatttta taattttagt aaaggagaag ggaatctaag caatattttt ttcttcattg
    10681 ctatagctag tattatattt tctccggttt caacaattat aggatcaatc ttaaattgga
    10741 attcaattaa accactttta ttaagaacct tagacatctt ggatgaggac ttagaaaaag
    10801 atggatcgga tactaaagta aacattctta gaggctcaat tcaattcaat aatatctctt
    10861 ttagttttga tgacaaaaaa attatagaaa acttaagttt gaacattaaa aataacgaaa
    10921 gtattgctat tgttggagaa agtggcagcg gtaaatccac tttggctaac ttattattaa
    10981 aaatgcatac tcccgataag ggtaatatat ttatagacga tagagatatt aaatattgga
    11041 acagtgagga attaagaaaa aagataggac taatgactca ggatggagtt ctatttagag
    11101 gaaatctatc tgagaattta tcttactttg atataaatta cgacccttca aaaattcagg
    11161 attctataga taaactaaat ctaagtaatt atattcaatt tcaaagtata aatgatattt
    11221 ttatatctga gggaggaaca aatttatccg gtggccaaag acaaaggtta gctatgttac
    11281 gcctgttcat gaaagaatat ccgattctta ttttagatga accaactaac catttggata
    11341 gagaaacggc aaatactgtt attgaaaata tatttgccat accatccact aagattatta
    11401 tcactcatga cgaaaatatt ttagagcgtg tagatagagt ttatgaactt actgataaaa
    11461 aattagaaca acgagaaata tcttctaaat ccagattgca aacattgggt aaaggataaa
    11521 tagatattta ataaatcaag aatttaatag gtacttaatt ttatttggga atgagaaagt
    11581 gaggaacaaa atgaaattag caattcaagc taaaaattta acaaagtctt ttgaaaatca
    11641 atccattgta agggatttaa acttatctgt tcctgaagga agtttatatg cttttattgg
    11701 accaaatggt gcaggaaaaa caacaactat gcgaatgtta ttaggattaa ttccattaga
    11761 taaaggaacg atattattta ataataaaga tattaaagaa tggaaagaag aattatttga
    11821 aaatgtgggt tgttttattg attcacctac ctattaccct aatttaacag catatgaaaa
    11881 tttagcctat gtacaaaaaa tgatcaataa acctttagat gaaatagata gagtattaaa
    11941 aactacagga atatataaag caaaagataa aaaagttaaa gatttttctt tgggaatgaa
    12001 acaaaggcta ggtatttcct ttgctattct taataatcct aaaatactaa ttcttgacga
    12061 gccgacaaat ggattagacc cagagggagt acacgagata aggcgtttat tacttcattt
    12121 agtaaaaaat gaggggaaga ctatatttat ttctagtcat aatttagctg aaattgaaat
    12181 gatagcagat cacatagcgt tactacataa gggtagctta ctctatgaag ggaatttaaa
    12241 cagtattatt acttctggag aatatattgt tcatgttaat gatactgctg ccacagagag
    12301 gattttaaat acagagggta taccttatag tatagaaaaa gaaggggtat tcaaaataat
    12361 agtagatcaa gatagtgttc ctaatttaac cagaataata attaaaaatg atatagatat
    12421 ttttgaagtt agccctaaaa agccaacatt agaggaagta tttttatctt taactaaaca
    12481 aaaggagtta ctataataat gcaatttatc tttaaaagtg taaatagtga gatgaggaaa
    12541 attggtggga gaaaattatt attaatttct ttgtttttct cattattatc aagctttttg
    12601 tgtataagtt attttatttt taataagaat aatcccgaca tgtttcaatc tacaaatctt
    12661 atagcaatta cagtaaactt taatacacta acgttaagta ttttttctgc ttgtatgtgg
    12721 tattttatca tttcttctga aaataaatat ggtacttggg ccattattta tacaaagccc
    12781 atttcgaaat tcaagttatt ggtagctaaa aatttattgt ttatactgtt ctatttgatc
    12841 tttaatattt tgaatttttc ggttatgggc atctatactt ttataaatgg atatgaaata
    12901 tcaatacttt attttttaaa aatgttgata ctttgtacta ttttatcttt agcaatacca
    12961 tattctcaat tgctttttca cttaattata aaaaagggta ttcttgcggc atccttgtca
    13021 gtaatttgga tattttttat cgtaacatat ggattattcc ctttaatatt tacaaagaca
    13081 tttccaatat tttatgcatt gaatatagta agtgacgtaa tgtttaaaga aacaactatt
    13141 ttattatatc ttgcaagttc ttcaatatat attttaggaa ttatgttatt gactgtgagt
    13201 tttaaatatt atacatatga ataataaagg gtgaaataag tgggtatttt aggtgaatat
    13261 aaaaaaagtc ttcataatgg attgtatatc ctgttactag tatttctgtt ggtaccaatt
    13321 agttttcaag tttttctaac tgtatcgcct caattaaatg atggcgcttt catgcatata
    13381 gcgattctga gccttttttg taggttattt ttacctattg cgataggtct attagtgtca
    13441 ttttcttttg taaacgaatt cagaagaaaa ggatacatgt ctgctattta tcatgggatc
    13501 actttaaaaa ctatattaag taataagttt ttcttttgct taatatttac tttttgttat
    13561 ttaatatgtg ctttaatcat aactagtata cctatagtat taacggttga taatctaggg
    13621 actgtttatc aaagtttaag tttttctata ctttttctgg taattagctc tttaattatt
    13681 gtgaatctac attttttaat aaatataata ttttataaaa tcccgttact ttcattatta
    13741 gtaggggtta taggtggact tggtagcttt tttatctctc atacaaacta ttggatacta
    13801 ttcccttggt catatccaat tatgagtctt tattttgata gcattgaacc aaaacaatta
    13861 tatattattc tcattgtata tctattatca ttactaggtt gtatatttat tattaaaaaa
    13921 tcaataagat ggaattaaag ttacttggca aatgacttca cctaaggaat tgagtctcgt
    13981 acactcaatt ccttttttat gttataaaaa aggagatcat ttgtcaaaaa gcatttgcta
    14041 tagaatagca aatgcttttt gttgaacttg aattgaacac tttcagaaag tatttatatt
    14101 gtttgccttt ttctcttgaa aaattgtacc ctcaattatt aattttaaca tagaatatag
    14161 aaaatgtagc gtttcaatat caaaaaaccc attggtttta tatggattat ttctttataa
    14221 taatgagagg tagttcccga atgtaaatag gctctcatgc aagaaaagca agagagccta
    14281 tctacataaa aaaagattgg atatttgcca atctttttcg tgtaagggat ttaatgatag
    14341 cttgttacta caactcgttt ttattttaaa acaattaacc agaaaattca atctaacgtt
    14401 ttttctcaat atggcttttc atatttttga attataatat aattaattct ttttatttct
    14461 atatctgtct cttatcttcc tgttgggaat ccttagtata aggattcttt ttttgttcta
    14521 attttgtaat ctaatctata tctgttcatt gctttataga ttaaaaggtc taatttttga
    14581 ctaacactaa ttactttttg atgtctaaaa ccatatgttt ttacaagata ctccaattct
    14641 ttttttttat cttctatcat ctcatgtagt aatttcatat ccactttttc ttttaaactc
    14701 ctttatacat agtatgatga aaaaactgta tttaaaaaat gcaaacaaaa aaacaagact
    14761 gaaaccagcc ttgccttaaa ggaaaaaact aactcttata tctaagagta cattatgtat
    14821 ggaaaatttt gaagtgtata tgcctaaaaa ggataaaaaa gttataattg taagattttg
    14881 aaagtttgtt attctataaa gaaacagttg atatcgcttg tttataaagg aacatttgct
    14941 ttccattgct taaaattagt accgaaaatt tatcaacagc gataattttt ccaggtaccc
    15001 tgacaccgtt tattagaaaa accgtcactt cttttttttc ttctattaac ttttcataaa
    15061 acttctcttg tgtatacatc attttgatgg ttgcaactcc tctccttttt cggaacggtt
    15121 catagcttct aagaatacat attcttttac tgttagctca accgttgggc aatattcaac
    15181 ctttgataat tcgtgaaata gagaaaggtt tttgtgtagt ttttgaatca tttcatctaa
    15241 agtgatttct cctacaccat actcaattac tgaatttaaa aatacgtcta catctattaa
    15301 acttggttca attagggaaa caatctcttg tacctttggt aaaataattg tttcataacg
    15361 ttgtttgaat agttctaaat cctctggtgc gggttcattc tcacaaagct ttttgaaaga
    15421 tagtacacga atgtcatttt gtaatgaatc aatacgctct tcataaaacg ctttgtcgaa
    15481 cccttctttg tcagcaacat cgtaatgaag ttctgtttgt gatctaggct ttttcttata
    15541 agcatccatg aggattttct cttttgtatt aaacatcatg ataaaaacag tacgttctag
    15601 attcatgatt tggtttgcta cgcttaatgt ttcatctaat acttttgcgc gttcttcttc
    15661 acttaacttt tttatagagg atggaacaag ttttttgaca ttctcaaacg actcggaatt
    15721 cataatgtgt tgaagagaaa attgagcgac ttctggtaaa caaggatcag ttgttttatc
    15781 cagaagtaac aaatctttaa tcgttcgcac tgtatttttg atttcttttt gagcttgtcg
    15841 aactttttct atgcttcttt ccatgtattc tggttgttta agacgattga aaatgtattc
    15901 tcttcttgct tcaaaatgtt gctgtaaaag tgacatagtt gtttcctcct ctattttctg
    15961 aattgatttg aaattccttt tatattgtat cattaaattt tatgaagtgg gacggtagca
    16021 aggtttattt gtaaagacaa ggtttggaat tgcatatcag acaaccaacg atgcatattc
    16081 tttaccaaaa caattaccaa agtggatatc aaccttataa ccaaagaatc tatcatgata
    16141 gtactatcaa aagaaatcat tgattatcac atgtttttag gggattttga gttttgttta
    16201 tagtaggaga aatggaatga gcaaaaggag gggaaacaat gaataattct tatgaaatta
    16261 tcggtaatca aattaaatat tatttgaggg atgatccaac gagaggctag aacaacatac
    16321 ctagtccgca atttcgaagt gtgaaagaaa ttacagaatg gtgtggaagt tcaacaaatg
    16381 aaattatgtg tgggtgggat gcgctcaatc aaaatgcaat gagtgtaacg gatacaacag
    16441 atttcttata taagtggact agttggtatg tgaccgaacc attagacagt acagcacaag
    16501 aaatacgaaa gttatcagac gaacttgcga gtttcataac acatgtacct gttgatacat
    16561 ttgaactcat taagaataac tctcatatgg tgttagattg tatatgtttt gtattggact
    16621 ggataccaaa tttgttaacg atattctttt ttttaggagg gaaaacatgc tgaatcgaaa
    16681 agaaacaatt tcttttcaag cctttatgga ccaatcttat aagaagaaaa agcaaacaaa
    16741 aatatttagt gttggcccta ttagtccgtt cgcatttttt catatgtctt caccgcttgt
    16801 tcatacgtat gtagccctgg gaatagtagg tggacttaca attggagctg ttttgttaga
    16861 gaaatactta gtacgaaatg attatgtttt tgcatccaat atcctttcta atggtctata
    16921 ttacggtgta agaattggcg ggattgggtt tattggttat gtatttattc gtattttact
    16981 catgttgtaa gggggatgga aaatgaaagg atggttaaaa aagaagagat acaaatacca
    17041 gttagaagag gtatttaaga aagcgaagtt atacgatgag cacgttacac gaggtgggaa
    17101 gctccctatt tatcctaaga ttcatgatgt ttacgcagga aaagaaagtg tacggtacac
    17161 ttttacacta cccaatggcg ttgatccagc tcaaattcat aagaaatggt tttgttttca
    17221 gcaaatacta ggtgaaaatc ttatgatgga aggtaatatt aaaaaatttg tactccatgt
    17281 atttcattct aatgcaagtt tacagcctta cgattacagt tataaacaat ggcaaccatt
    17341 attaaaacaa tatcgtcttc caatcgtggt tggtcgggat caatttggta atacgattgt
    17401 gtatgatatg gttgattcaa acacaccaca tttactgatt gcgggagaaa caggaagcgg
    17461 aaaaagtagt atagtacgtg tgattttagc cacattaatc caacagatgt caccagagca
    17521 tttacatttg tacctgggag acttaaaaaa ttcagagttt cattttttaa gaagagtgaa
    17581 gcatgtgaag tatgtttgca tggaagaaca tgaaatgacg aacatgttag caaaattatg
    17641 gaaagaggta ttgcatagaa gaaaggtaat ggaaaaatat gagctaggtc atattgatga
    17701 atacaatcaa ataacaaaag ataaaccctt accatatatt tttattgcca ttgatgaagt
    17761 agcaatgcta caggacgaaa aggaatgtgt caccataata gaaaaaatat cagctgtcgg
    17821 cagatcgctt ggtatcttcc tcatgctaag tatgcaacgt ccagatgcaa aaatacttga
    17881 tggcaagtta aagctcaatt tgacggtaag aatgggcttt aaatgtgcgg atttaattaa
    17941 cagtaacatt gtagggacgc ctggatcaga aaatttaagt caatcgggcc aaatgatttt
    18001 aaaattagat ggtttaaaga aggtgcaggc tccttatttg gcattggacc aagcgaaaga
    18061 aataatagaa ccgtatcgtt tatctaaaga gcaaagtata aagaaggaag aaccagaaca
    18121 tcaagaagac aaaatctttg gggtgttaga tgatgctaac gaatagagat aaacaaatta
    18181 taggtcattt acagcaattt agatgtatgt cgcgtgatga tattattgat cttcatttta
    18241 gaaatgtaaa gaatgcagta acatgttgta atactgttac aaagcgttta agccgcgaag
    18301 gatatatcga tgtggataca tcacaacgtc catatgtgta ttttccttat ccaaatacaa
    18361 ttcgtaaagg aagccaaaag atacgtcatt ttttagcgat tgtagatgta tataagcagt
    18421 tattacgaac cgaaagaccg aagatatttc aagtagaacc taaatataag aaaggttata
    18481 tggaacccga tatatttacc atttggcata aaagtccatt ttttatagaa gtccagaact
    18541 ccatttataa taaggctacg atccaaacaa aaatcaaacg ttatgaatcg tattttcgta
    18601 gcatggaatg gaaaaaagaa tcctggcaac cgaaacataa tatggtattt ccctcacttt
    18661 taattcttac tgacctcaaa tatccgattt ctagttctaa tttccgtatt tttcaagcgc
    18721 cctctattca gcattttctt atacaagtac agaaacctaa agacatttcg gttcaaatag
    18781 gttcagtcaa agcaaaatta tgaaagcaca agatactgtt tcttgtgctt tttacatgtc
    18841 ccaaaagtca ttgattttgg cgtgaggatc aatttttcta atagtattta atattttttt
    18901 catgatttca ggtttgggga tgtaatctgt atctctacat gctttactga tggtttgaac
    18961 agaaaccttt gactttttgg atagttcctg ttgttcaatt ccttgccgat ctaaccatct
    19021 ttttaattta gtcctatttt tgcggaaaaa catttatctc actctcctgt ctttaatttt
    19081 ttatttacaa ttatggacag gaagttaaat tcatatacaa ttcaattcaa cttaaactta
    19141 agtaagatac aagagaacga gattcataga gtatttgcac gaacgattta ttgataattt
    19201 cacttggaat gaggtataca tacaatgcaa agatttgact tacgcatcat acaacaaatc
    19261 tttgcaaagc actttccaaa aaaaattaaa ttcgtctatt cttagagaac atgaataagg
    19321 acaattttgt tttactcgta taaggtaagt aattaaaaat tggataaata gacagggttt
    19381 ggacatatta cattttgagt at
//
