LOCUS       NZ_KB976300            10261 bp    DNA     linear   CON 14-JAN-2024
DEFINITION  Bacillus cereus K-5975c achrv-supercont1.1, whole genome shotgun
            sequence.
ACCESSION   NZ_KB976300
VERSION     NZ_KB976300.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus cereus K-5975c
  ORGANISM  Bacillus cereus K-5975c
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5209568)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C.,
            Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T.,
            Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     The Genome Sequence of Bacillus cereus K-5975c
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5209568)
  AUTHORS   Feldgarden,M., Van der Auwera,G.A., Mahillon,J., Duprez,V.,
            Timmery,S., Mattelet,C., Dierick,K., Sun,M., Yu,Z., Zhu,L., Hu,X.,
            Shank,E.B., Swiecicka,I., Hansen,B.M., Andrup,L., Walker,B.,
            Young,S.K., Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B.,
            Abouelleil,A., Alvarado,L., Arachchi,H.M., Berlin,A.M.,
            Chapman,S.B., Dewar,J., Goldberg,J., Griggs,A., Gujja,S., Hansen,M.,
            Howarth,C., Imamovic,A., Larimer,J., McCowan,C., Murphy,C.,
            Neiman,D., Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T.,
            Sisk,P., Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2012) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: allpaths v. R38749
            Assembly Name                     :: Baci_cere_K-5975c_V1
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/14/2024 01:11:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,396
            CDSs (total)                      :: 6,275
            Genes (coding)                    :: 5,989
            CDSs (with protein)               :: 5,989
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 5, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 286
            CDSs (without protein)            :: 286
            Pseudo Genes (ambiguous residues) :: 0 of 286
            Pseudo Genes (frameshifted)       :: 113 of 286
            Pseudo Genes (incomplete)         :: 198 of 286
            Pseudo Genes (internal stop)      :: 95 of 286
            Pseudo Genes (multiple problems)  :: 94 of 286
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:03:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2882836
            Orig. end                         :: 2893097
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB976300.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Please be aware that the annotation is done automatically with
            little or no manual curation.
FEATURES             Location/Qualifiers
     region          1..10261
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10261
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10261
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5261](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5261)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    742..755
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.32"
                     /tool="antismash"
     gene            complement(938..1381)
                     /locus_tag="IGY_RS14515"
                     /old_locus_tag="IGY_02890"
     CDS             complement(938..1381)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="IGY_RS14515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IGY_02890"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_016077589.1"
                     /transl_table=11
                     /translation="MKKVNVTYAILYDKTNEKILMVKNKGENGFYYTLPGGAVKLGETL
                     EEAVIREVKEETGLHINVKGICSISEAFFEERDHHAIFFNFLGEIIGGETYISRPKEIE
                     EITWMELHIAAPYLRIPEHLLDLLQKKETVPYFFNGTIVHQSS"
     misc_feature    1462..1480
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.4"
                     /tool="antismash"
     gene            complement(1663..2088)
                     /locus_tag="IGY_RS14520"
                     /old_locus_tag="IGY_02891"
     CDS             complement(1663..2088)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706722.1"
                     /locus_tag="IGY_RS14520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IGY_02891"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_000706899.1"
                     /transl_table=11
                     /translation="MKIVQQWVQEDSDYIREKVIEYNQKYLADEEKTPSEKVSFIVRNE
                     KEEIVGGVTAIIFWHHVHVDFLWVSEEYRHEGYGNKLIKLIEEFAIEKGCRLINLDTFS
                     FQAPDFYKKHGYKVIGVSEDHPKGHNHYYLEKRLKSI"
     gene            complement(2219..3427)
                     /locus_tag="IGY_RS14525"
                     /old_locus_tag="IGY_02892"
     CDS             complement(2219..3427)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="IGY_RS14525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IGY_02892"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_000814378.1"
                     /transl_table=11
                     /translation="MKQSSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQKKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDNLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNAVTETLHNDYTPREMMRTKQVYLLFFMLFTS
                     CMGGLYLISMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIILGTLSDKIGRMKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGAILGGFQPTFIIIGVLSVISFIISILIRPPTAEKKKELKHLHR
                     KVA"
     gene            complement(3708..3851)
                     /locus_tag="IGY_RS34230"
                     /old_locus_tag="IGY_02893"
     CDS             complement(3708..3851)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="IGY_RS34230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IGY_02893"
                     /product="hypothetical protein"
                     /protein_id="WP_016077590.1"
                     /transl_table=11
                     /translation="MKDKVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFGEI
                     EY"
     gene            complement(3883..4899)
                     /locus_tag="IGY_RS14535"
                     /old_locus_tag="IGY_02894"
     CDS             complement(3883..4899)
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="IGY_RS14535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IGY_02894"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_016077591.1"
                     /transl_table=11
                     /translation="MYTYEKLISWVENIKEKNQSSAAALCIIKDNKIVLEHYSGYHSNT
                     STSKKVTAPSQFNVASARKSYLGLIIAYALYEGKINSIDDEAIKYFKDFDPVLLGKTTI
                     RHLVTHSHGLEETNDGTIFREFEPGQSWAYRDINVRMMTLLIYQLYNKSFPELLKERVF
                     KPAKFQETGWRIQQDENLVEIINNPNEDPISGIGTVNDGTEKNLFVSAREFAQWGNLHL
                     NQGMIHDKQIIPKEVIKIATSLQSPTFINKELPQNGVFWFIQNEPAQLSELGERIPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSNRIRA"
     gene            complement(5001..5261)
                     /locus_tag="IGY_RS14540"
                     /old_locus_tag="IGY_02895"
     CDS             complement(5001..5261)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071342.1"
                     /locus_tag="IGY_RS14540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IGY_02895"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_016077592.1"
                     /sec_met_domain="TIGR03601 (E-value: 6e-43, bitscore:
                     143.4, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQHELQTLNLNDYQTGNVVYWDQQQSQYPYYYIQDDARRCGG
                     CGGRCGGCGGGRCGGCGGCGGRCVGCAGCFGCFNCWNWWII"
     gene            complement(5461..5907)
                     /locus_tag="IGY_RS14545"
                     /old_locus_tag="IGY_02896"
     CDS             complement(5461..5907)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="IGY_RS14545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IGY_02896"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_000836826.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAVMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELREELGVNCSIQQFLGVIENQWQDKEVLHHEINHIFEVESQDLHTDLPPKSSESH
                     LAFQWIDCNKEALNHYEIMPMPLVKELLERKLSDELLNCWISNF"
     gene            complement(5924..6271)
                     /gene="cutA"
                     /locus_tag="IGY_RS14550"
                     /old_locus_tag="IGY_02897"
     CDS             complement(5924..6271)
                     /GO_process="GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="cutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160776.1"
                     /locus_tag="IGY_RS14550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IGY_02897"
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="WP_001160789.1"
                     /transl_table=11
                     /translation="MQFTEVKIEVFIPEEYIETLRDELNKIGACKTGEYDHCLSYSSVK
                     GYWRPLDEASPFNGEIGRICEGQECKVEIKCKRELVKDALEVINDIHPYETPMIYIIPI
                     LNDYFEQLYIE"
     gene            complement(6302..6760)
                     /locus_tag="IGY_RS14555"
                     /old_locus_tag="IGY_02898"
     CDS             complement(6302..6760)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="IGY_RS14555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IGY_02898"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_001102680.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEEALKLQVKENQLKFVPSVAVSLAKVYIKPDG
                     DNVEYIPFAIYVGDLMVGFVMHAVVRKTSDMYWINGFIIDQKQQGNGYGKAALQESINI
                     IKNTFKSCKEIRLTVHKDNISAKKLYERYSFKSLEQEYDGEQVYRLFV"
     gene            complement(6838..7761)
                     /locus_tag="IGY_RS14560"
                     /old_locus_tag="IGY_02899"
     CDS             complement(6838..7761)
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="IGY_RS14560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IGY_02899"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_000416781.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATKPLTEEITTYYPEIHGAEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGKDLTIVDVGRSTSLAIAFNLWNDLMLDVKAIYFMGGVFLEVGNVTPLA
                     EANVYGDPIASKIVFHQAKNLLIFPLNVTNKAVLTPNVFSYIQANTKNPFHMIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVVAISGLVNPSFFEFVHRTVNVDTYGDTKGQTFADFRPS
                     SIPEGAHIALEIDEQKFIQDFIKIML"
     gene            complement(7924..9285)
                     /locus_tag="IGY_RS14565"
                     /old_locus_tag="IGY_02900"
     CDS             complement(7924..9285)
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017763222.1"
                     /locus_tag="IGY_RS14565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IGY_02900"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_001013608.1"
                     /transl_table=11
                     /translation="MNGKRNIFTCISIIGIGLASFSSFSFAANVTDNSVQNSIPVVNQQ
                     VAAAKEMKPFPQQVNYAGVIKPTHVTQESLNASVRSYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMIITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADSKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGTVNYLKEAQDMITKGIKAS
                     NVTNNSRLNLGDWDSKNSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFSN
                     KYSPNTGLISDFVVKNPPQPAPKDFLEESEYTNAYYYNASRVPLRIVMDYAMYGEKRSK
                     VISDKVSSWIQNKTNGNPSKIVDGYQLNGSNIGSYSTAVFVSPFIAASITSSNNQKWVN
                     SGWDWMKNKRESYFSDSYNLLTMLFITGNWWKPVPDDKKIQNQINDAIYEGYDN"
ORIGIN
        1 cgtttgaaga aattttaatt gtagattcgt ttatgatttc taagttaaca ttgttattgg
       61 cccattcact tgctaataaa cttgatcgat ctacaccttg taatatcttt tttctatcta
      121 tgttaataat cgttttaaag tcatttggaa taaggttaga aatattagga tatttccctt
      181 caattagtct tgaatataat gtagttgttc caagcgtaaa tataatatga ctctctgaaa
      241 ggtaaatata tacgaattcc gaattgctgt tcattaattt tagaagttca ctaatggttg
      301 cgcttggtac gatacaattt gcttttacat ctgaggaaag tcgcgtttca cgtatcgcta
      361 gtctatgtga gtcagttgca gcacaaataa acttattatt agataactct atatgcaccc
      421 cagtaagaac aggtctagat tcatttttcg ctaccgcaaa gactgtttgt ttaaatactt
      481 caatcaattg ctctgtctct accttgattt ctgcatgatt gtctataagc ggtacattcg
      541 gaaactcatc tgcaggaaat ccatttaaac ttaatgtgat ttcttctgat tgtatcttaa
      601 taatttgctc attcttactt tttattgcta tatcacttgg cattttcttt attatttcaa
      661 tgaaatactt tgcaggtaca acaatagttc ctgcctttaa aatagttgca attttttcgt
      721 cttcaattga aacaggaatg aatttttcaa taaaaatatt cgagttactt gcaattaaag
      781 taattccaga ttgatctgca gttattttta taccagataa tataggaatt aaagattttg
      841 ttgatatcgc tttgttaact tctgaaagtg cttgagtaaa ttgtttgtga ttaacgatga
      901 actccatgtt ttccccgcct tacatgagat aatattttta tgaagattga tgaacgattg
      961 ttccgttaaa aaaataaggt actgtttctt tcttttgtaa taaatctagt aaatgttctg
     1021 gtatgcgtaa atacggtgct gctatatgta attccatcca agtaatctct tcgatttctt
     1081 ttggtcttga tatatatgtc tccccgccga ttatttcgcc tagaaaatta aagaaaattg
     1141 catgatgatc tctttcctcg aaaaaggctt cgctgataga acaaatccct tttacgttta
     1201 tatgtaaccc tgtttcttct tttacttctc gaattactgc ttcttccaat gtttctccta
     1261 atttaactgc gccacctggt aatgtataat aaaaaccatt ttctcctttg ttttttacca
     1321 ttaagatctt ttcgtttgtt ttatcgtaca aaatcgcgta tgtaacatta acttttttca
     1381 tatgtacctc ctctattttt aataaaagga attttctgtc ttctatccat tctaccatta
     1441 actcctttta atatatataa gttagttaag tcttgccttt accgctattg gtggagttaa
     1501 taagaatggg ccactgatat ataaagaaag aacgtatctt aggaagatta tccaaagaac
     1561 gttcttgaag ttttagcatg aaaattgatt aaattaaaac taaaattaat aataacctcg
     1621 ccttgattac atttgtattc caaatactaa tgttaacaaa ttctatatac tttttagccg
     1681 tttttctaaa taataatgat tatgtccttt cggatgatct tcacttactc caattacttt
     1741 atatccatgt ttcttataaa aatcaggagc ttggaaacta aaggtatcta aattaattaa
     1801 cctacaccct ttttcaattg caaattcttc aataagcttt attaacttgt ttccgtaacc
     1861 ttcatgccta tattcttcag aaacccatag aaagtcaacg tgtacatggt gccaaaaaat
     1921 tattgctgtt accccaccta cgatttcttc tttttcattt cttactatga aactaacctt
     1981 ttctgaaggt gttttttctt catctgcgag atatttttga ttatattcaa ttactttctc
     2041 tcttatgtaa tcactatctt cttgtaccca ctgctgtact attttcattt taagtgtcac
     2101 gctccttttt acttatctat tcaattttat aatgatattg tcctgtcttt aatgagttag
     2161 taaagaagca aagttaatgg aataaaaaag agccctaaca tgaggactct ttttaaaatt
     2221 aagcaacttt ccgatgtaaa tgttttagtt cctttttctt ttctgcagtt ggtggacgga
     2281 ttaatattga aataatgaag gaaataacac ttaaaacacc gattataatg aaagttggtt
     2341 gaaatccccc gagtatcgct ccaataaatg atcctgcaag cgcaccaaat ccgaaacctt
     2401 ggtagacaat gccgtaattt gtactatggt tttttaatcc aaagaaatct ccgacaatag
     2461 ctgggaatat agttatatta ccaccgaagc aaaaggcgac acttgctaca caagcaaaat
     2521 agattccgta atttagcgga ataaaactta aagtaaagac tgacaatcct ataataataa
     2581 acgttgcaga gacaattttc attcggccga ttttatctga tagcgtccca agaataattc
     2641 tacctactgt attaaagatt gcaatcatag caacggcgtt tgcagcggtt gctgcgctaa
     2701 gtccaacgag ttgtacgccg atatctttta ccatacttat taaatacaaa ccacccatac
     2761 acgaggtgaa taacataaaa aataggagat atacttgttt cgttcgcatc atttcacgcg
     2821 gagtatagtc attgtgtaat gtttcagtta cagcattact tacaattgcc tcacgtaaga
     2881 agaacgatcc gattaacact aaaagtaaca cgataatacc ccaatataag aatgcttgtg
     2941 atacaccaag gttatcgata agatttccgt ttatatatct aaagattaag ctgcccattc
     3001 catacgctga tacagatata cctgaaataa gccctttccg atttggaaac catttaatta
     3061 aattagatag tgatgtaata taagctgttc catccgcata accaacaacg acaccagcta
     3121 ataaatataa tagtggtaaa gaagaaactt gtgaactaag tattaagccg agtcctaaaa
     3181 caattcctgc agtagcaata agttttcgaa gtcctaattt tttctgcaac tttgctgcaa
     3241 ataaagttga gaatgataaa gaaaaacttg ttatggagaa agttatcgct actgaattaa
     3301 gattccatcc aaacttactt acaaggggtt gattaaataa actccatgta taaattgttc
     3361 caaggccaat ttgaacaatg attgtaccta gaacaattag taacggattt atagaacttt
     3421 gtttcatact aacttgtccc cttcttttat cttgacatga attttcacag ttagtatatc
     3481 catcataaat tacaagatac tgtacggaaa atggtaatta agttcatata cacaaaaaga
     3541 cacttcataa agaagtgcct tttgcaggtt atatcagata tttctaaaga aagctttgtt
     3601 aagataagac attttcaaat gcttatgtat atatgttatc aaataatttc ttaaatgtta
     3661 tgagacttaa gacgtatttt tcattcatca tatagtcgga tgaaaaatta atattcgatt
     3721 tcaccaaaat ctttcggctg cggtacagtt tgcatattag ctgcatcttt aggatcgcta
     3781 taaattgcat tttgctctgt atttacatct tttatatttt gctttttatt tttgttcact
     3841 ttatctttca ctttcaatca ctcctttttg ttataggagt aattatgccc taatacggtt
     3901 actaaatgta tcagcgacta aattactaaa ttcacgtaaa taatataagt aattatcacc
     3961 gccgtagtta tatcttttat tatacatttt tgcaacaact acattatatt caggtattac
     4021 taaaatagtc ggtccagtaa tccctaatat ttgatacgac ccttttggaa tacgttcacc
     4081 aagttcgctt aattgtgcag gctcattttg gatgaaccaa aacaccccat tttgtggtag
     4141 ctctttgttt ataaatgttg gactctgcaa actcgtagca atttttataa cttcttttgg
     4201 aatgatttgt ttatcatgta tcatgccttg atttagatga agattgcccc actgcgcaaa
     4261 ttctctagct gagacaaata gatttttttc agtaccatca tttactgtac caattccact
     4321 tatcgggtct tcatttggat tattaataat ttcaaccaaa ttttcatctt gctgtattct
     4381 ccatcctgtt tcttgaaatt tagcaggttt aaaaacacgc tcttttaaca attcaggaaa
     4441 acttttgtta tatagctgat aaataagaag tgtcatcatt cttacattaa tatctctata
     4501 cgcccatgat tgtcccggtt caaattcacg aaaaattgtc ccgtcattcg tttcttctaa
     4561 tccgtgcgaa tgtgttacta aatgtcttat cgtcgtttta ccaagcaata caggatcaaa
     4621 gtctttaaaa tattttatcg cttcatcatc aatagagttt attttcccct cataaagcgc
     4681 atacgctatt attaatccta aataactttt tctagcagaa gcaacgttaa attgtgaagg
     4741 tgctgttact ttcttgcttg tagatgtatt tgagtgataa ccactataat gctctagcac
     4801 gattttatta tcttttataa tacaaagtgc tgcagcagaa ctttgatttt tttcttttat
     4861 attttctacc caagaaatca acttttcgta tgtatacaaa taaatccccc ttaacactaa
     4921 tacaaaatta cagcatgaaa gaaaagtata aacagtattt acacctttct ttcatataaa
     4981 atattaatga tgtactatat ttaaataatc caccagttcc agcaattaaa gcaaccaaaa
     5041 caaccagcgc aacctacgca acgtccacca cagccaccac agccaccaca tctaccgccg
     5101 ccacaaccgc cacagcgtcc accacaacct ccgcaacgac gtgcatcgtc ttgaatgtag
     5161 tagtatggat attgactttg ttgttgatcc caatacacaa cattaccagt ttgataatca
     5221 ttaagattta atgtttgtag ttcatgttga aactgattca ttaagaaccc ctccatttat
     5281 aaatataaat tccgcccaaa tattaaagtt aataaattct tgtgaacaaa gtagaaacaa
     5341 gactatagac taaatgcgta catcaacaaa atatgaactt acactaggtg atgtaccttg
     5401 tttttcagcc tattttcttt atattcattc gatggacgta tttactataa ataaagtgat
     5461 ttaaaaatta ctaatccaac aatttagtag ttcatcactt aactttcttt ctagtaattc
     5521 cttcactaaa ggcattggca tgatttcata atgatttaag gcttctttat tacaatctat
     5581 ccattgaaat gctaaatgag actcgctaga tttcggtggt aagtccgtat gtaaatcttg
     5641 tgactctact tcaaaaatgt ggttaatttc atgatgaagc acttctttat cttgccattg
     5701 attttctatg actcctaaaa attgttgtat actacagttt actccaagtt cttctcttaa
     5761 ctctcttatt aatgcattct ctgctgattc tccaatttca acatggccac caggtaaaaa
     5821 ataatgatgg ccaatatatt cagcaactaa taatttttca tcttttatca tgactgctcg
     5881 tacaatatga tgaaatttac ttttcaattg atattcctcc tagttattca atgtatagtt
     5941 gctcaaaata gtcatttagt attggtataa tataaatcat aggtgtttca tacggatgaa
     6001 tatcattaat aacttcaaga gcatctttta caagttctcg tttacacttt atttcaactt
     6061 tacattcttg cccttcgcat attcgaccaa tttctccatt aaaaggagat gcctcgtcta
     6121 acggtctcca atatcctttc actgaactat aggaaagaca atggtcatat tcgcccgttt
     6181 tacatgctcc aattttattt aattcatccc ttaatgtttc aatatattct tcaggaatga
     6241 acacttcaat tttcacttca gtaaattgca tatttcattt cacgccttta cttcctaata
     6301 gttaaacaaa taaacgatat acttgctcgc catcatactc ctgttctaat gatttgaaac
     6361 tgtatcgttc atatagtttc tttgcagaga tattatcttt atgtacggtt aatctaattt
     6421 ctttacacga tttaaaagta ttttttatta tattaatact ttcttgtaat gctgcttttc
     6481 catatccatt accttgctgc ttttgatcaa taataaatcc gttaatccaa tacatatctg
     6541 atgtctttct tacaactgca tgcattacaa aaccaaccat aaggtcacca acgtatatag
     6601 cgaatggtat gtactctaca ttgtcaccat ctggttttat atatacttta gcaagcgaaa
     6661 ctgctacgga tggaacaaat ttcaactgat tttctttaac ttgtagcttt aatgcttctt
     6721 cccaattttc tctcgtaaca acctttaact ggacattcaa tattgtaagc tcctttatct
     6781 agagggatgt atttttatat tcaacgcatt gaaataattc cctttttcga caacaactta
     6841 taacatgatt tttataaaat cttgaatgaa tttttgttca tctatttcta atgctatatg
     6901 ggccccttca ggtatagagc tagggcggaa atcagcaaat gtttgtcctt tcgtatctcc
     6961 gtatgtatct acatttactg tacgatgcac aaattcaaaa aaacttggat ttactaatcc
     7021 acttattgca accacatcat gtagcaaagg tccttcgata gatggattaa gtttttggta
     7081 tgcagataaa tagtagtcat acattggttt cattatcata tgaaatggat tctttgtatt
     7141 agcttgaata tagctaaata catttggtgt taacacagct ttgtttgtta catttaaagg
     7201 gaatattaat aaattctttg cttgatggaa aacaatttta cttgctatag gatctccata
     7261 cacatttgcc tctgctaacg gcgtaacgtt tcctacttct aaaaaaacac cacccatgaa
     7321 atatattgct tttacatcta gcattaaatc attccataaa ttaaaagcaa ttgctaacga
     7381 ggtagatctt cctacatcaa caatcgttaa atcttttccg tactttacaa gaatcgcagc
     7441 gatactacca aaattgtata ttggaataga taatacgctt tctggaacat ggatgggccc
     7501 taatccttct gctccgtgaa tttcagggta gtacgtagta atttcttctg taagtggttt
     7561 tgttgcacca ctaatgacag gaatgtcttg tctatttgct aactgtaaaa tatacgctgc
     7621 attatgtgca gcatgtatat gctcaacatt tccgtatcca gttacaatac ctacaatttc
     7681 aatttcggga tgcagtaaac catacataat ggcaaatgag tcatcaatac ctgggtctcc
     7741 gaaaaataat actttttcca agaaatactc tcccctcata aaaacataga gtcatcaata
     7801 actatgtata tgaaaaaagc aagtatatct atgcgaattt tagaatggat aatgttttac
     7861 aaaatattaa aaaggaacta gcgtatgcta attcctttct taagtaacca ttctcaaatt
     7921 taattaatta tcgtatcctt cataaattgc atcatttatt tgattttgta tttttttatc
     7981 atcaggtaca ggtttccacc aatttcctgt aataaataac atagttaata aattataact
     8041 atcactaaaa tagctttctc tcttattctt catccaatcc caaccgctat ttacccactt
     8101 ttgattattg ctactcgtta tacttgcagc aataaacggt gaaacaaata cagcagttga
     8161 ataactacca atattagatc catttaattg ataaccatcc acaattttag aaggatttcc
     8221 attcgtttta ttttgaatcc atgaagagac tttatcagaa atgactttac ttcttttctc
     8281 gccgtacatc gcatagtcca ttacaattct caatggtacc cgactagcat tgtaataata
     8341 tgcatttgta tattctgact cctctaagaa gtctttaggt gcgggttgtg gtgggttttt
     8401 tacaacgaaa tctgaaataa gtcctgtatt tggagagtac ttattactaa attgcgtata
     8461 aacatcgtac aaattattaa taacagtaag ccaagtttta tcacctgtaa attcataaaa
     8521 tgctctaagg tgtgacatca tccaatcaga tggcctcgta tcaagtgaat ttttagaatc
     8581 ccaatctcct aaatttagtc ggctattatt tgtaacatta ctagctttaa taccttttgt
     8641 aatcatgtct tgtgcttctt ttaaataatt aactgttcca tttgatcccc actgcttatg
     8701 agcaagaaga agagaatacg caatatctaa atccccatca gtagcagaat caaaatgacc
     8761 ttgtgctttt ttactatctg cgacaaccca tcccattaaa ttaggatttt gagagctttt
     8821 aaaagttctt gctgttttaa ataaaccatc atagattttt tgggcattcg aatcgtaacc
     8881 agccattaat actgtaatta tcatcccata cccttgacct tctgaagttc caagtggctt
     8941 aaacccatcc gcatcacccg taatctctcc ttttacgtaa taaccaccag gtaaagaaga
     9001 taaatcattt ttcaaatatt tctttttcca attatcgtag taacttctta cagaagcatt
     9061 taaactttcc tgtgtaacat gagtaggttt tataacacct gcataattaa cttgctgggg
     9121 aaatggtttc atttcctttg cagcagctac ttgttgatta actacgggaa tagaattttg
     9181 tactgaattg tccgttacat ttgctgcgaa actaaaacta gaaaaactag ctagaccgat
     9241 tcctataata gaaatacatg tgaaaatatt tctttttcca ttcattatgt tgtcatctcc
     9301 ttttaaaatg cttactacat aaaatgtaac atacaatatt taactgaaat acaaaatatt
     9361 atatttatca acataagaaa gaaactatat gaaaaagtat cataaaatta ttattgaatt
     9421 acgttatacg gattgattat ttgctaaatt cctatgtttt tcttttctta atactggtat
     9481 atgagcaatc acaataccaa gtatagtaat ccctccgcca attatcgaaa ctaaagttgg
     9541 cacttctcct aaccataacc aagcaataaa acatgcagtt actggggtta aatatagaga
     9601 gcttgttgcc tcagcagcac cagcatgaga tatgatatag gccaatgcga tatatgggag
     9661 tactgttgga aataagccta aatataaaac actcacattc acttcaagag gagctgctag
     9721 tatttcttga tacattcccg gtaagaaaat aagcatacat acagtactag ataaaattgt
     9781 atatatggta aatggtaaga agccgtactt ttttaagtaa gaagtttgaa aaacaaaata
     9841 taggctttct gaaatcgctg ctaataatat aaataatccc ccactattca attgaatagc
     9901 atctccttga ctaaatgata tgagagcaat tcctataaaa ctaattacac caccgatcca
     9961 accatttaat ttcatttttt cgtttaaaaa tatagaagca agaattgccg ttactatagg
    10021 tgtgaccgaa acaattaaac ttgcagaacc agcatttact gttttttcgc cgtagtttaa
    10081 tgcaatgtga taaaaagtga atcctaaagc accgaacata aaaattgctg ggatatcctt
    10141 taaatccggt agccgtaact tgtagataaa agaaaacaac agaagtataa atgaagcgat
    10201 taataagcgt agtaaagtaa gatgctcagg tgtataagat tcaagcccca tacgaattgc
    10261 c
//
