LOCUS       NZ_KB944820            11044 bp    DNA     linear   CON 12-JAN-2024
DEFINITION  Enterococcus faecalis EnGen0359 strain TR197 acAqV-supercont1.6,
            whole genome shotgun sequence.
ACCESSION   NZ_KB944820
VERSION     NZ_KB944820.1
KEYWORDS    WGS; RefSeq.
SOURCE      Enterococcus faecalis EnGen0359
  ORGANISM  Enterococcus faecalis EnGen0359
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 590638)
  AUTHORS   Earl,A.M., Gilmore,M.S., Lebreton,F., Walker,B., Young,S.K.,
            Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A.,
            Alvarado,L., Arachchi,H.M., Berlin,A.M., Chapman,S.B., Dewar,J.,
            Goldberg,J., Griggs,A., Gujja,S., Hansen,M., Howarth,C.,
            Imamovic,A., Larimer,J., McCowan,C., Murphy,C., Neiman,D.,
            Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P.,
            Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     The Genome Sequence of Enterococcus faecalis TR197
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 590638)
  AUTHORS   Earl,A.M., Gilmore,M.S., Lebreton,F., Walker,B., Young,S.K.,
            Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A.,
            Alvarado,L., Arachchi,H.M., Berlin,A.M., Chapman,S.B., Dewar,J.,
            Goldberg,J., Griggs,A., Gujja,S., Hansen,M., Howarth,C.,
            Imamovic,A., Larimer,J., McCowan,C., Murphy,C., Neiman,D.,
            Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P.,
            Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2013) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: allpaths v. R42411
            Assembly Name                     :: Ente_faec_TR197_V1
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/12/2024 01:36:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,260
            Genes (coding)                    :: 3,176
            CDSs (with protein)               :: 3,176
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 3, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 47 of 84
            Pseudo Genes (incomplete)         :: 36 of 84
            Pseudo Genes (internal stop)      :: 25 of 84
            Pseudo Genes (multiple problems)  :: 20 of 84
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:10:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 249662
            Orig. end                         :: 260706
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB944820.1.
            Please be aware that the annotation is done automatically with
            little or no manual curation.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11044
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11044
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11044
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6044](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6044)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            39..902
                     /locus_tag="WOK_RS19100"
                     /old_locus_tag="WOK_02985"
     CDS             39..902
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /locus_tag="WOK_RS19100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_02985"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_010715444.1"
                     /transl_table=11
                     /translation="MELQHLEYFLVLTRFNNFTKASKYLGITQAALSQQIKNLEEELGA
                     PLFIRKKNKNILTPAGKVLESYCWKIKQQIYYMKDELADNSDNSETLPSVKISLSLNDL
                     EFHLVDTLISIFTDIKVTPIISDNVIDDILTLKSDIGITPTIDTRKIPACINAIHQYSH
                     DYVIIVRKNHPILNKKFDINSLILYPFVFFSPHCKESPKIYEWIETNLKTIKNIINVDS
                     IELVLRLVYGSDSFSIVPDYLIPQLIRLNIKYIKYNDLPRREISVYLLKEKYLNRNIRK
                     IFNTLL"
     gene            complement(2242..2943)
                     /locus_tag="WOK_RS0104120"
                     /old_locus_tag="WOK_02986"
     CDS             complement(2242..2943)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365254.1"
                     /locus_tag="WOK_RS0104120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_02986"
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="WP_029659359.1"
                     /transl_table=11
                     /translation="MAETDDPNVPPTPPAETKVPIEPTIHKNIEGVQKLVLKNNKQQFN
                     WNIAVYFGTNTMNWKQASVTDAVNNVLDIVKVTVVDENGKDVTGNGALSTENNKVIFEI
                     NKKDDSYTYLAGHTYTMTITTKIKDSVTDEELVPYIKDGGIPNQANLNFGNKGDKKHSE
                     IPTIVPPKGMSPEKPSTPNNKNDKDNLTSSKGIFTHTGENQMLSNILLVVGTGILIILV
                     VYYILKKRRIE"
     gene            complement(2880..3290)
                     /locus_tag="WOK_RS19115"
                     /old_locus_tag="WOK_02987"
     CDS             complement(2880..3290)
                     /GO_process="GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /locus_tag="WOK_RS19115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_02987"
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="WP_010826759.1"
                     /transl_table=11
                     /translation="MPPTPPAETKVPKVELTKTADKANAKVGEEITYTITAKNSGNGYW
                     ENGTITDKIPTEYVNLISGTTVVNGEKVEDNEVWKDNVLNVKQTIKAGESVTITFKVKT
                     IKGAEGGKRLRMWQRQMIQTYHQLHQQKQKYL"
     gene            complement(3731..4321)
                     /locus_tag="WOK_RS19120"
                     /old_locus_tag="WOK_02988"
     CDS             complement(3731..4321)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002394296.1"
                     /locus_tag="WOK_RS19120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_02988"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_002394296.1"
                     /transl_table=11
                     /translation="MKEGKAMIEIKDLNIPYVDKKISMNILKEYNLFTGDNGNGKTLLL
                     DYISGVKKAKRTVIKGNESIIYINQNIYFSDRLSGMDFLKFSYGLDGKKSIQSFYQLSD
                     KILKREDIDKLLKKQWGMLSGGEKKFLYALILMSLEREWYILDEPFAFVDKKRKKMLYK
                     IIDIKISEDKGIILTTHEEDEVVLKSVSKVFEI"
     gene            complement(4308..4988)
                     /locus_tag="WOK_RS19125"
                     /old_locus_tag="WOK_02989"
     CDS             complement(4308..4988)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405757.1"
                     /locus_tag="WOK_RS19125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_02989"
                     /product="hypothetical protein"
                     /protein_id="WP_002365261.1"
                     /transl_table=11
                     /translation="MFISVKSYALFNIKMLLKDKIPLLWSIALPVITFFMNYRSVTNEW
                     ELAYWWVYIVVCSYVYGVGVYALQLKEYGSLKTIFSINNSPWAFFLGNLLTQIMFSFIS
                     VNLLNLIVVILLPLSFLRLFLYSFITIIACLPVAFFSYNLTLIKKVHANTINSIFTMLI
                     FGLFILLNTNIELNKYNPLSILSNVLIEHKNMDVFKYVTFSIFLVFISMYSIVKFEPIS
                     NERR"
     gene            complement(5001..6044)
                     /locus_tag="WOK_RS19130"
                     /old_locus_tag="WOK_02990"
     CDS             complement(5001..6044)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401935.1"
                     /locus_tag="WOK_RS19130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_02990"
                     /product="YcaO-like family protein"
                     /protein_id="WP_010826760.1"
                     /sec_met_domain="YcaO (E-value: 5.4e-08, bitscore: 28.0,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGFLRNKVNFVLNEDLFMSHHYNVYIEKYSKRPIKAYEVDLKNK
                     RQHILSTLGEYLERETLINENSPKEHTVIATNLIDGSIKQVNLDMVLFKNSFVDSSGMA
                     SHKKSKPLIWTAYKEFFERQSYITNFLFHTKAQQLNIDKIEEIKKIDRYLTNYLDNVKY
                     YNISLSKNLYVVLAVGYGEKYKAVGLGTSQSARKAVIKSQKEILQYFATSHSKYKEEKV
                     SEASGLVIDNKDIYHDYFDRLSSKEVKDLYAYLEGSEFIILNNEEKIEKVSHTFTIKNI
                     SNSLNMSPYITMFENNHAKNVKVVKVFDPKWFPNMAPRNFSKEIILNVENILKLKRKNF
                     SELLPFP"
     gene            complement(6062..7039)
                     /locus_tag="WOK_RS19135"
                     /old_locus_tag="WOK_02991"
     CDS             complement(6062..7039)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365263.1"
                     /locus_tag="WOK_RS19135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_02991"
                     /product="hypothetical protein"
                     /protein_id="WP_002365263.1"
                     /transl_table=11
                     /translation="MIFTNKSSYGVKSDMFLSNINIGYVNFSRVKIKQGTTAGSAFSVD
                     ENKQLSLIKSYNEYLERLFLGIPLSTNKTISSVNLEKNIVSSRKYSEFGYGNHEFGFND
                     TTGTSSGKLSEVIIQKALTELIEKNEVFCFWYGLKGEKLKQDKEIHNLINDYNFISNMF
                     KIYVVRELSNYSTVIVMGFLEGKLLTSGVCCSITMEQSLILALKEAKGIEWQTFNNPLA
                     KANKFSDKLHKQILKKVKFMDETYKVITKDSIQATKYIETADWINHVEVSVIGDDINRG
                     VKTIKCISKQLLNSVPIKINIERQKDKEIIKRYLIDYSIDCPIQ"
     gene            complement(7023..7679)
                     /locus_tag="WOK_RS19140"
                     /old_locus_tag="WOK_02992"
     CDS             complement(7023..7679)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401933.1"
                     /locus_tag="WOK_RS19140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_02992"
                     /product="hypothetical protein"
                     /protein_id="WP_002399151.1"
                     /transl_table=11
                     /translation="MSDYYWNEYKNVLLDEAYLIETLKYQQRVMNKQVEENNLDELSDV
                     LGLYNVKYSDIRLAVYPFDELPAFVKTLVSDLQKNYASFKYKVSMPILAITKKIGNVNA
                     GCYSFDFKNNFLILYETDVTKIREIVNDKLVLAFFLNIEDAICLYKKKAYTNGIKEIGY
                     VSKSNQLAISKEKNIIINEMLVPEQQLTNGLGINLRKCLLMEALILEVLTNDIHE"
     gene            complement(7672..8502)
                     /locus_tag="WOK_RS19145"
                     /old_locus_tag="WOK_02993"
     CDS             complement(7672..8502)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04424"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379875.1"
                     /locus_tag="WOK_RS19145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_02993"
                     /product="McbB family protein"
                     /protein_id="WP_002365265.1"
                     /sec_met_domain="TIGR04424 (E-value: 5.4e-89, bitscore:
                     293.7, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYKVNPFIWHDLSEKETFFQTKLSSIIITNKNLVRMLKDLEKNEV
                     INFEESLLSNYFRSNMINNVLVFLEENKLIKKLDIENNKVNEVLVYTDDSEFSELFDYV
                     FSEKFTISHQSSISHLESKNMDGKLLIVFLKAFNFKEYENIVNIAKEKNVVIKIIFSYN
                     HHIYFSNFYKREWYNPCPICFFSELESQLRGEITSDTANFQTIIDLLYTKSQNFAIEYP
                     LQKDDYFTIVYTIMRNLDSDLMKCDLDEVLSIDLNNHLVTKDIAYHWGYCDCYE"
     misc_feature    complement(8543..8554)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            complement(8567..8770)
                     /locus_tag="WOK_RS19150"
                     /old_locus_tag="WOK_02994"
     CDS             complement(8567..8770)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405754.1"
                     /locus_tag="WOK_RS19150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_02994"
                     /product="hypothetical protein"
                     /protein_id="WP_002410046.1"
                     /transl_table=11
                     /translation="MNVEEVLVSQKTPNAINEETELYGFGYGSVVENITVSKGGGGGGG
                     GGGGGCCCCCCCCCGGATGLEE"
     gene            complement(9401..10714)
                     /locus_tag="WOK_RS19155"
                     /old_locus_tag="WOK_02995"
     CDS             complement(9401..10714)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /locus_tag="WOK_RS19155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_02995"
                     /product="NCS2 family permease"
                     /protein_id="WP_010826761.1"
                     /transl_table=11
                     /translation="MKEKISSYFELEQLNTNMKREMLAGFTTFISMAYILFVNPTVLGA
                     SGMDEGAVFTATALASALGCILMGVLAKYPIATAPALGINAFFAYSVSVGMGIPWQTTL
                     AGVFVASLIFILITIFKLRELIIDAIPADLKFAISGGIGLFIAFLGLSQGGLIVANDAT
                     LVGLGPLNVGSTWVTIVGLVVTAILLVRRVPGGIFIGMAISTIVGLATGVIPMPDKIIS
                     SAPSLEPTFLVALGHVKEINTIQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMKDNKMP
                     RVGKALAADSTAMLAGSLLGTSPVGAYVESSAGIAVGGRSGITAITTGILFIFSLLFSP
                     LLAVVTSQVTAPALIIVGVLMAQSLSKIKWNEMEIAIPSFLILLGMPLTYSISDGIALG
                     FIFYPITMIAAKRGKEVSPIMYGLFFVFVGFMWILNAQ"
ORIGIN
        1 cattttttta ttttaaattt tattagcgga ggttgtatat ggaattacaa catttagaat
       61 actttttagt cttaactagg tttaacaatt ttacaaaagc tagtaaatat ttaggtataa
      121 cacaagctgc tcttagtcag caaattaaaa atttagaaga agaattagga gctcctttat
      181 tcatcagaaa aaaaaataag aatatattaa ctcctgctgg taaagtttta gaatcgtatt
      241 gttggaaaat aaaacagcaa atttactata tgaaagatga attagctgat aattctgaca
      301 attcagaaac acttccttct gtaaaaatat ctttatcatt aaacgattta gaattccatc
      361 tagttgatac tttaatttct atatttactg atataaaagt tactcctata atatctgata
      421 atgttattga tgatatattg acgttgaaaa gtgatattgg tattacccca actattgata
      481 caagaaaaat acctgcatgc attaatgcga tacatcaata tagtcatgat tacgtaatta
      541 ttgtgagaaa aaaccatcct atattaaata aaaaatttga tatcaacagc ttaatcttgt
      601 atccctttgt atttttcagt ccacattgta aggaatctcc aaaaatttat gaatggatag
      661 aaactaattt aaaaactata aagaatatca ttaacgttga ttctattgaa ctggtattac
      721 gattagtgta tggtagtgat agcttttcaa tcgttccaga ttatctaatt cctcagttaa
      781 ttcgtcttaa tataaaatat ataaaatata atgacttacc aaggagagaa atatctgtat
      841 acctattaaa agaaaaatat ttaaatagaa atatcaggaa aatatttaat acactcttat
      901 aataaatata ttgagtaagt agattaacct tattatctta gtagatatat tctcagtatt
      961 gtctaatgca ccaaatagaa gcagacttta tttatcaatg tttactaaaa attattttga
     1021 tggataaaac ctaattttct acgataatta tatatcttta ccaaaaatag tactttaaga
     1081 atgcctatta ttcctacatc aattaaagta atgagctaag acaattacaa tatttcaaca
     1141 aatttagtga ttgtttgtcc gtccgattcc gataacccaa ttaagtaaag aacatccttt
     1201 ttttatctat agcgaaaaac tttatactac agataattgc agtaagtaaa gcgaacacgc
     1261 ttaaagtata ttccacctta ctatacaaaa aaggcccaat ataaggtaaa gtatgtagat
     1321 acgttggacg aagaaatatt ttatgagatt gctcaaaagt tttcacaaaa tttttttgtt
     1381 atttaaaaaa cttcaattga agttcactct ttacagaagc aaagaactaa agatttttct
     1441 atatacagtc acagtcgttc gctatataaa taaattcaac cagctgttac acaactgtcc
     1501 tttattgaaa aggcttctcc ttaataattt aaaagatttg tgtttattgt tatcttttca
     1561 atatcttaca aattgaagat cctaaaatat tttatattgc attaaattct tccaagttta
     1621 ttttttatat taacgtgata ctcattcatt cttaatatta atgaaatctc aattttttta
     1681 cataaaatag ttaacagctt tatcagctga tccttatacg atttttcact aatattagta
     1741 ttccttatat aatttttttg ttttaacatt taaaataaat ttagacatag cggtttatcg
     1801 tttggtattt tataacattt caagtaagcg taactatact tttgtgcgta tatcaaatta
     1861 gattaataga taaaataaag catactctct ataaaaaaac agagaatatg ctttacgact
     1921 tttcagtatt aagacaacat agaatacttt taatcaaaaa ttagtttata ttcagtttta
     1981 attcttccaa attcaattgt ttataccttt tttatttcat catgccattt taaaatactt
     2041 attacattta tacgatttcg attaataaca tccatattca taattataag taattcatct
     2101 aaagatattc cttctggata gcacaacaaa atctctttta tttcatcaaa atttagtttc
     2161 atactataca tcttaataag tattagaaca gcctgtatac tatcaagtaa tataaactgt
     2221 tctaactata ctaaaaatta actattcgat tcgtcttttt ttaagaatat aatatactac
     2281 aaggattatt aaaatacctg tccctacaac gagtaatata ttacttagca tttgattttc
     2341 tccagtatgt gtgaaaatac cttttgaaga agtaagatta tctttatcat ttttattgtt
     2401 tggagtacta ggtttttctg gagacatccc ttttggtgga actattgtag gtatttctga
     2461 atgcttttta tctcctttat taccaaagtt taaatttgct tggttaggta ttccaccatc
     2521 tttgatgtat ggaaccaact cttcatccgt tacactgtct ttgatttttg tggtaattgt
     2581 catggtatac gtatgacctg ctaaataagt atagctatca tcttttttat taatttcaaa
     2641 aataactttg ttgttttctg tacttagtgc accgttccca gtcacatctt taccattttc
     2701 atctactact gtaactttca caatgtctaa tacattattt actgcgtctg taacggaagc
     2761 ttgtttccaa ttcatagtat tggttccaaa ataaacagca atattccaat taaattgttg
     2821 tttattattc tttagtacta acttctgtac accttctata ttcttgtgaa tagtaggttc
     2881 tataggtact tttgtttctg ctggtggagt tggtggtacg tttggatcat ctgtctctgc
     2941 cacattcgca atcgtttgcc cccttctgct cctttgattg tttttacttt aaacgtaatt
     3001 gtgacacttt ctccagcttt aattgtttgt tttacattta atacattgtc tttccaaact
     3061 tcattatctt ctactttttc accatttacg actgtcgttc cacttatcaa gttcacatat
     3121 tccgtcggaa ttttatcggt gatcgtgcca ttttcccaat agccattccc actatttttc
     3181 gctgtgattg tataggtgat ttcttcgcct actttagcgt tagctttgtc ggcagttttt
     3241 gttaactcta cttttggtac ttttgtttcc gctggtggag ttggtggcac atttactaaa
     3301 taaaaaagga acagttatct attaacaatg tagtgcgccc ccaaagttag actaaaaaat
     3361 ctaactttgg gggcgcacta tacaatgact gttccttttt ttattatttt tattcaactc
     3421 caatatagaa attaaaatta agattcctat tattttaata gagacttgac ctccacagtt
     3481 tgcttgcttt aataaaaagc aagcaccact aacgcaaaag ctagaaatca ttactttttg
     3541 aacttaatta ctttgcaaaa aaaaacattg caaatcaacc agtactatct atactttcca
     3601 aaaaactact atacaaaaac ataattattt tatatttact ataatttaaa ctacggtctg
     3661 agcttattat aattcattct tttatcgtac aacattaaat attctaaaat gatgatacta
     3721 cttcttacag ttatatttca aaaaccttgc taactgattt taatactact tcatcttctt
     3781 catgagtagt taagataata cccttatctt ctgatatctt aatatctata atcttatata
     3841 acattttttt tcttttctta tctacaaaag caaacggttc atctagaata taccattctc
     3901 tttctaaaga catcaaaata agagcataaa ggaacttttt ttcccctcct gaaagcattc
     3961 cccattgttt tttaagcaac ttatcaatat cttcacgctt aagaatttta tcgcttaatt
     4021 gataaaaaga ttgaatactt ttttttccgt ccaaaccata actaaatttc agaaagtcca
     4081 ttcctgataa acgatcagaa aaatatatgt tttgattaat atatatgata ctttcattcc
     4141 ccttaattac agttcttttt gcctttttta ctcctgaaat gtaatctaat agtaaggttt
     4201 ttccgttacc attatcacca gtaaataaat tatactcttt taaaatattc atacttattt
     4261 ttttatctac ataaggaata tttaaatcct ttatttcaat catagcttta ccttctttca
     4321 tttgaaatcg gttcaaattt aactatagaa tacatactaa taaaaactaa aaatattgaa
     4381 aaagtaacat atttgaagac atccatattt ttatgttcaa ttaaaacatt agataaaatt
     4441 gacagagggt tatatttatt taactctata ttcgtattta acaatataaa taaaccaaaa
     4501 attagcattg taaatattga atttatagta ttagcatgca ctttttttat taaagttaaa
     4561 ttgtaactaa aaaatgcaac tggtaaacac gctataatag taataaagct atataaaaaa
     4621 agacggagaa acgataatgg tagtaaaata accacaatta aattaagtaa gtttacactg
     4681 ataaagctaa acataatttg cgtcaataga ttgcctaaaa aaaatgccca aggtgaatta
     4741 tttattgaaa aaattgtttt taagcttcca tattccttta attgtaatgc gtaaactcct
     4801 actccataaa cataggaaca aacaacaata tatacccacc aatatgccaa ttcccattca
     4861 tttgttacac ttctataatt cataaaaaaa gtaattacag gtaaggctat tgaccacaat
     4921 aaagggattt tatctttcag taacatttta atattaaaca atgcatatga tttaactgag
     4981 ataaacattt tcactcctcc ttacggaaat ggtaacagtt ctgaaaaatt ttttcttttt
     5041 aattttaata tattctctac atttaatatt atttctttcg aaaaatttct tggtgccatg
     5101 ttgggaaacc actttgggtc aaatacctta actactttca cattcttagc atgattattt
     5161 tcaaacattg taatatacgg agacatatta agagaattgc ttatattttt gatagtaaat
     5221 gtatgactaa ctttttctat tttttcttca ttattcagaa taataaattc actaccttca
     5281 agatatgcat ataaatcttt aacttctttc gagctcaatc tatcaaaata gtcatgataa
     5341 atgtccttat tatcaattac taatccagag gcttcactaa ctttttcttc tttatatttt
     5401 gaatgcgatg tagcaaaata ttgtaatatt tctttttgtg attttattac tgcttttctt
     5461 gctgattgac tagttcccaa gcccactgct ttatattttt caccataccc aacggcaagt
     5521 actacgtaaa ggttcttact taaagatata ttataatatt tgacattgtc taaataatta
     5581 gtcaaatatc tgtctatttt ttttatttct tctatcttat ctatatttaa ttgctgagcc
     5641 ttagtatgaa acaagaagtt agtaatatag ctctgccttt caaagaattc tttatacgct
     5701 gtccatatta atggctttga ttttttatgc gatgccatac cagaagaatc aacaaaacta
     5761 ttcttaaaca aaaccatatc aagattaact tgtttaatgc ttccatcaat taaattcgta
     5821 gcaataaccg tatgctcttt tggtgaattt tcattgatga gtgtttctcg ctccaaatac
     5881 tctcctaaag ttgaaagaat atgctgcctt ttattcttta aatcaacctc ataggctttg
     5941 ataggtcttt tagaatattt ttctatatat acgttatagt gatgtgacat aaataaatcc
     6001 tcatttaaaa caaaattcac cttatttctt aaaaaacctt tcaaaagtat tctcctttct
     6061 atcattggat aggacaatca atagaataat caattaaata tctcttaata atctctttat
     6121 ctttttgtct ttcaatgttt atcttaattg gtacagaatt tagtaactgt ttggatatac
     6181 attttattgt tttaactcct ctatttatat catctccaat tacggatact tcaacatggt
     6241 taatccaatc agcagtttca atatatttcg tagcttgtat agaatctttt gtaataactt
     6301 tgtatgtttc atccataaac tttacttttt ttaaaatttg tttatgcaat ttatcagaaa
     6361 atttatttgc ttttgctaag ggattattaa aagtttgcca ctctattccc ttggcttctt
     6421 tcaaggccaa aattagagat tgttccatag taatactaca acatacacct gaggttagta
     6481 atttaccttc taaaaacccc atcacgatca ctgttgaata attactaagt tcacgaacta
     6541 cataaatttt aaacatattt gaaataaaat tataatcatt aattaagtta tgaatttctt
     6601 tatcttgttt taacttttcc cctttcaaac cataccaaaa acaaaaaact tcattttttt
     6661 caatcaattc tgtgagtgcc ttttggatta ttacttctga aagttttcca gatgaagttc
     6721 ctgtcgtatc attaaatccg aactcatgat ttccatatcc aaattctgaa tattttctgc
     6781 tacttacaat atttttttct aaatttacag aagaaatagt tttatttgta cttaatggta
     6841 tccctaagaa aagtctttct aaatattcat tataactttt aataagtgat aactgtttat
     6901 tttcgtcaac tgagaaagca ctacctgctg tcgttccttg cttaatcttg acacgcgaaa
     6961 aattcacata tccaatattt atatttgata aaaacatatc acttttcaca ccgtaggaac
     7021 ttttattcgt gaatatcatt agttaacacc tctaaaatta aagcttccat taacaaacat
     7081 tttctcaagt ttatacctaa tccatttgtc agttgttgct ctggtactag catttcgttt
     7141 attattatat ttttttcttt actaatcgct aattgattgc ttttagaaac ataaccaatt
     7201 tcttttatac catttgtata cgcctttttt ttatataaac aaattgcatc ctctatattt
     7261 aaaaaaaaag ctaacactag tttatcattt actatttctc taatcttagt tacatcagtt
     7321 tcatatagaa tcaaaaaatt atttttaaaa tcgaaagaat aacaaccagc atttacattc
     7381 cctatttttt ttgttatggc tagtattggc atactaactt tatatttaaa ggaagcataa
     7441 ttcttttgta aatcactaac tagcgttttt acaaaagcag gcaactcatc aaaaggatat
     7501 accgctagcc taatatccga gtatttcaca ttatagagac ctagaacatc actcaattca
     7561 tctaaattat tttcttctac ttgtttattc attacccttt gttgatattt taacgtctca
     7621 attaaatatg cttcatctaa aagtacattc ttatattcat tccaataata atcactcata
     7681 gcaatcacaa tacccccaat gataagcaat atcttttgta actaaatgat tatttaaatc
     7741 aatagataaa acttcatcta agtcacattt cattaaatcg ctatccaaat tcctcattat
     7801 ggtataaact attgtaaaat aatcatcctt ttgcaacggg tattctatag caaaattttg
     7861 tgattttgta taaagtaaat ctataattgt ctgaaaatta gctgtatcag aagttatttc
     7921 tcctcttaat tggctttcta gttcagaaaa aaaacatatt ggacatggat tgtaccattc
     7981 ccttttatag aaattcgaaa aataaatatg atgattatag ctaaaaatta ttttaatgac
     8041 tacattcttt tccttggcta tatttacaat attttcatat tctttaaaat tgaaagcctt
     8101 taaaaatact ataagcaatt taccatccat atttttagat tctaagtggg aaatactgct
     8161 ctgatgacta attgtaaatt tttcggaaaa cacgtaatcg aataattcag aaaattcaga
     8221 atcatcggta tataccaaaa cctcattaac cttattattt tctatatcta gctttttaat
     8281 taatttattt tcttctaaaa agaccagtac attattaatc atgttgctcc taaaataatt
     8341 ggataataga gattcctcaa aatttattac ttcatttttt tctaaatctt ttaacattct
     8401 aactaaattt ttattagtaa taattatcga agatagtttt gtttgaaaga atgtttcttt
     8461 ttcactaaga tcatgccata taaaagggtt tactttatac attttttcac tcctttttac
     8521 ttgattatgt aaagaggtaa gtgcgtccac ttaccttatg aagtttctat tcttctaatc
     8581 ctgtggcacc cccacaacaa cagcagcaac aacaacagca gccaccgcca ccgccgcctc
     8641 caccaccgcc tcctccttta gagacagtaa tgttctcaac gacagaacca tacccaaacc
     8701 catataattc agtctcttca ttaattgcat ttggggtttt ttgcgacact aaaacttctt
     8761 caacattcat tttaatacac ctccgttata attttatcaa tattgatgaa attattttac
     8821 cactatccaa tcatttttca agcaaaaact catcttattc taattaaaaa acgtatattt
     8881 atactgaaaa atctaaatat ctttaattag acaacaaaaa caaaaaaaat aataacaaaa
     8941 caactatcat taatttttct atttttatat caatgaaaat tctaacaaaa taatttttaa
     9001 aatatttatt ttatcacatt aaaattataa tatacaatgc atattcgcat atttttggat
     9061 tttacctctt taataacaaa acatatttaa gtatttaaaa agcaacaacc gctaacacaa
     9121 gagctaacaa tcattgcttt ttgaacttat ttactgtacg aaaaaagaca ttaaaaacca
     9181 gtattctcaa tacatttcaa cagaaactac tacctgaaat cataatttct gtatatttca
     9241 ctcaattaat tcctctctgt tcctgtatct tcacaggtca atgtctttgc aaattataca
     9301 tcaaataagg ggcgtttttc tagtgcttag tcgaaaaaaa atagttgatt cataagttca
     9361 ttgtcttatg aatcaactat ttgataactt tctctcttgc ttattgcgca ttcaggatcc
     9421 acatgaatcc gacaaaaaca aagaacaagc catacataat tggcgaaact tctttcccgc
     9481 gttttgccgc aatcattgta attggataga agataaagcc taaggcaatc ccatctgaaa
     9541 tgctatatgt caatggcatt cctaacaaga ttaagaagga agggattgca atttccattt
     9601 cgttccattt aattttgctt aaggattgtg ccatcaaaac accaacaata atcaacgctg
     9661 gtgctgtaac ttgcgaagtg acaactgcta acaaaggaga aaatagcaag ctgaaaataa
     9721 ataaaatccc ggttgtaata gctgtgattc ctgaacgacc acccacagca atccccgcag
     9781 atgattcgac atatgcgcca actggcgatg tccctagtag agaaccagct aacatcgctg
     9841 ttgaatcagc ggctaaagct ttccccacac gcggcatttt attgtccttc ataaaacctg
     9901 cttgattggc taagcccacc aacgtaccag ccgtatcaaa gaatgttact aataggaacg
     9961 ttaaaaccac gacccacatt tgaatcgtat tgatttcttt cacatgtcct aacgccacta
    10021 aaaatgttgg ttctaaactt ggcgcgcttg aaataatctt atcaggcatt ggaataacgc
    10081 ctgtcgctaa accaacaatc gttgaaatcg ccattccaat aaaaatacca cccggcactc
    10141 gacgaactaa caaaattgca gttacgacta agccgacaat ggtgacccac gttgaaccaa
    10201 cattcaaagg tcctaagcca acgagtgtcg catcatttgc cacaattaag ccaccttggc
    10261 ttaaaccaag aaaagcaata aacaacccga tcccacctga aatggcaaat ttcaagtctg
    10321 caggaattgc atcaataatt agctcgcgta atttaaaaat tgtaattaaa ataaatatca
    10381 atgaagccac aaaaacgcct gctaatgtcg tttgccaagg aatccccata ccaacagaga
    10441 ctgaataagc aaagaatgca ttaattccta aagctggtgc tgtcgcaatc ggatatttcg
    10501 ccaacacacc cattaaaata caacctaagg cactcgctaa agcggtggca gtaaaaacag
    10561 cgccttcgtc catgccagaa gcaccaagta cggttggatt aacgaacaaa atatacgcca
    10621 ttgagataaa tgtggtgaaa ccagctaaca tttctcgttt catattcgta ttcaattgtt
    10681 caagttcaaa atatgaactt attttttctt tcatgttccg tcctcctaaa atctaaacat
    10741 tttacaatgt tcgtaaaata ttcaatttat taatgaaaca acgtgtttaa tcatataata
    10801 aaccaacaaa taagtaaata tcaaattcta aattctgtaa aaaacgaaca atcacgatta
    10861 aaaaaagtat tcctttctag gaatactttt taaattaaat cgtttgtgtg gtcgggcgaa
    10921 caaccatttc actgattgac atacgatctg gcgtgtctat ggcaaagacc accgcttgcg
    10981 caatgtcttc cgcctttaag ccccaactta actgttctag atgtaacgtt tcagctacta
    11041 ctcg
//
