LOCUS       NZ_KB944819            10288 bp    DNA     linear   CON 12-JAN-2024
DEFINITION  Enterococcus faecalis EnGen0359 strain TR197 acAqV-supercont1.5,
            whole genome shotgun sequence.
ACCESSION   NZ_KB944819
VERSION     NZ_KB944819.1
KEYWORDS    WGS; RefSeq.
SOURCE      Enterococcus faecalis EnGen0359
  ORGANISM  Enterococcus faecalis EnGen0359
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526810)
  AUTHORS   Earl,A.M., Gilmore,M.S., Lebreton,F., Walker,B., Young,S.K.,
            Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A.,
            Alvarado,L., Arachchi,H.M., Berlin,A.M., Chapman,S.B., Dewar,J.,
            Goldberg,J., Griggs,A., Gujja,S., Hansen,M., Howarth,C.,
            Imamovic,A., Larimer,J., McCowan,C., Murphy,C., Neiman,D.,
            Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P.,
            Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     The Genome Sequence of Enterococcus faecalis TR197
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1526810)
  AUTHORS   Earl,A.M., Gilmore,M.S., Lebreton,F., Walker,B., Young,S.K.,
            Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A.,
            Alvarado,L., Arachchi,H.M., Berlin,A.M., Chapman,S.B., Dewar,J.,
            Goldberg,J., Griggs,A., Gujja,S., Hansen,M., Howarth,C.,
            Imamovic,A., Larimer,J., McCowan,C., Murphy,C., Neiman,D.,
            Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P.,
            Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2013) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: allpaths v. R42411
            Assembly Name                     :: Ente_faec_TR197_V1
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/12/2024 01:36:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,334
            CDSs (total)                      :: 3,260
            Genes (coding)                    :: 3,176
            CDSs (with protein)               :: 3,176
            Genes (RNA)                       :: 74
            rRNAs                             :: 4, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 3, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 47 of 84
            Pseudo Genes (incomplete)         :: 36 of 84
            Pseudo Genes (internal stop)      :: 25 of 84
            Pseudo Genes (multiple problems)  :: 20 of 84
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:10:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 752686
            Orig. end                         :: 762974
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB944819.1.
            Please be aware that the annotation is done automatically with
            little or no manual curation.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10288
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10288
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10288
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5288](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5288)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(372..1595)
                     /locus_tag="WOK_RS13940"
                     /old_locus_tag="WOK_01939"
     CDS             complement(372..1595)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634400.1"
                     /locus_tag="WOK_RS13940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_01939"
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="WP_029659325.1"
                     /transl_table=11
                     /translation="MILKKLTKKQQISLTIIGCLVGVLLISATLIYKLNATEPKEEEAY
                     QIIKLKKSDPLVLKGIVQPKTTSYLNFDQSLGKINTISVKNGQEINENDVVATYQNTTV
                     EDQAEEQTQSLEKLNLAVTNAQINLDNATQKQQELENRLTIAKNEQATIVNKKMDDEMK
                     KAEKTESDSKIETIQQALDSQKEAVLQAKQALDAANVDLSSANNTIEQTKKKITTTVTA
                     PFKGIVYINDKGKVDATVPYATIVSPETVIKGSVTEYDYNKVKVGQPVTISQINEEKAT
                     DGIITEINALPEEMAVSPQNTSSTKNSTISTFSFIISPKEPIHYGYNVQISVPMNNLEL
                     AKKNTVKENNNEVFVFIYRDGKVVKQKIEVKEDNDKYVVKTGLKENDSIIENPDTSLKD
                     GQKVTVKQ"
     gene            complement(1573..2082)
                     /locus_tag="WOK_RS13945"
                     /old_locus_tag="WOK_01940"
     CDS             complement(1573..2082)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634401.1"
                     /locus_tag="WOK_RS13945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_01940"
                     /product="hypothetical protein"
                     /protein_id="WP_010709544.1"
                     /transl_table=11
                     /translation="MEIYSKYIKKIRIWNLITLVLASIGTIFSIIGLPAALSPDKTLYK
                     DLGLEGMSIYNQLNSMPVKVFTVLNVLFTIAFVILFFISHRKIKKDQIPLKYPYYLNLG
                     WTVVSIIFSLLTTPSFTNTSETQSMGLIIGITTTIFTLVFKLPIIMVLVYLFKVNTGEN
                     DIEKVN"
     gene            complement(2123..2644)
                     /locus_tag="WOK_RS13950"
                     /old_locus_tag="WOK_01941"
     CDS             complement(2123..2644)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407591.1"
                     /locus_tag="WOK_RS13950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_01941"
                     /product="hypothetical protein"
                     /protein_id="WP_025190992.1"
                     /transl_table=11
                     /translation="MSGLNTKKNNWLILVFFIPYLVTNVFLFLTLIGKDNKLLEMIAES
                     SKLLNISDEKFNLFMIVLVVIVNLFIFFVTFIILKIVLLILGFKKNYDQDIFISLLLSI
                     SLVNLLAIFAAQIININRVPLSISTSTIEVIVFLLLFYNNTKDIKATKFLFYGKLVLLL
                     INIGSLIILK"
     gene            complement(2616..3242)
                     /locus_tag="WOK_RS13955"
                     /old_locus_tag="WOK_01942"
     CDS             complement(2616..3242)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368634.1"
                     /locus_tag="WOK_RS13955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_01942"
                     /product="hypothetical protein"
                     /protein_id="WP_010709546.1"
                     /transl_table=11
                     /translation="MKSVIELENVGKMYKEKNIFSDISIKFLNSMYFCRGSNGSGKSVF
                     LRCLGNIENFSTGTFKHNNKINLFLTDQSLCHYYLTIIENIELFFSIHGLELTPQKLEQ
                     IKSLYNEEKQLTTLAENASLGMLLKAACTLLFEKGHWDLVILDETFSSIDKQSRTILLE
                     QCHQLILDGTCVVLVSHNELELEYKYNYFTINLDKSGERIEYKEK"
     gene            complement(3239..3748)
                     /locus_tag="WOK_RS13960"
                     /old_locus_tag="WOK_01943"
     CDS             complement(3239..3748)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368636.1"
                     /locus_tag="WOK_RS13960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_01943"
                     /product="stage II sporulation protein M"
                     /protein_id="WP_010777735.1"
                     /transl_table=11
                     /translation="MKKENEFNLKGYFLFFLIASLYLVSVYFIFDQFDIPSEVNGISIK
                     SIDYGVRYALKIIFNNTKNFLQYILLFPIVPLLILYELFMISFQTWISVNNVGGFETFQ
                     LLYKHGIIELPNMFLYMFLSLNCCTIFFKNFSIIDVLDFIKQNKKTYVFSYMLICISGI
                     IEGMIG"
     misc_feature    3726..3740
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.3"
                     /tool="antismash"
     gene            complement(3762..4937)
                     /locus_tag="WOK_RS13965"
                     /old_locus_tag="WOK_01944"
     CDS             complement(3762..4937)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390744.1"
                     /locus_tag="WOK_RS13965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_01944"
                     /product="hypothetical protein"
                     /protein_id="WP_010777736.1"
                     /transl_table=11
                     /translation="MGNIYLKIFKRKLRIVENFHIFKLFFLIMYVCTCTLLLLIINLNN
                     LLLPIILINFLLLNYNLPKIELELNQMTSYNYFYSDKVFIKYILFLINKNNPFFSMVFL
                     INLFNIILNITNGLLVINILTAFFLQLILFLIQLLAKRVYKIFTSIILIILLLGLLFSK
                     GFITSVLSFLLVIFLLLFVYFDVFVRRKFKSFDLKYVRNNAKITMSKIFTSKNMYNQLF
                     ITYLSRVSIKEYFEYLFICCLLIMYSSFFNFIFDNTGLLIFFSLIDMELLSDRHFKYLS
                     KTSAEFYFLSNSPVQKNKTYFLSNYFHKSLIFVMIGMVSLIFSMDRKLTVILINFIYLS
                     IVIFCISFLYYKLKNKGIFTRKKENNLVVQYILIIFILLSLMAKTYIIKSL"
     gene            complement(5001..5288)
                     /locus_tag="WOK_RS13970"
                     /old_locus_tag="WOK_01945"
     CDS             complement(5001..5288)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368637.1"
                     /locus_tag="WOK_RS13970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_01945"
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_010709549.1"
                     /sec_met_domain="TIGR03651 (E-value: 6.8e-16, bitscore:
                     54.8, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLNNVLFNKKMYMGVAFVLVAFAISLTQPHLTSTLGISAYAAKK
                     VIDIISAASSVAAVVGIIAAVVGGGGIGVAVLATAKALVKKYGKAYAAAW"
     gene            5719..5949
                     /locus_tag="WOK_RS13975"
                     /old_locus_tag="WOK_01946"
     CDS             5719..5949
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /locus_tag="WOK_RS13975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_01946"
                     /product="transposase"
                     /protein_id="WP_010709550.1"
                     /transl_table=11
                     /translation="MIDKRKSPRKFDEEFKNSIVKLYENDKSQNSLAKEYGLAVSIIVR
                     WIKQFSEVKFDDGSILTARQIQQLQKRNMQL"
     gene            6387..>6545
                     /locus_tag="WOK_RS21230"
                     /old_locus_tag="WOK_01947"
                     /pseudo=""
     CDS             6387..>6545
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /locus_tag="WOK_RS21230"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_01947"
                     /product="IS6 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAHFEGKQFKQDIIMVAVGYYLRYNLSYLAISEIMSDRGVMVCHT
                     TIYRWGQE"
     gene            complement(6728..7042)
                     /locus_tag="WOK_RS13980"
                     /old_locus_tag="WOK_01948"
     CDS             complement(6728..7042)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="WOK_RS13980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="WOK_01948"
                     /product="hypothetical protein"
                     /protein_id="WP_010709552.1"
                     /transl_table=11
                     /translation="MGTKDLVVAWNSMDEDDRFELESFEQVVALSYVKNLVSEDESLQF
                     TYANGNQAAIDLFDVERFRYVPHDSHLAQYVRSKAKVDHEWDEQGNVLTNEKENRLLKK
                     "
     gene            complement(7073..7252)
                     /locus_tag="WOK_RS21980"
                     /old_locus_tag="WOK_01949"
     CDS             complement(7073..7252)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /locus_tag="WOK_RS21980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_01949"
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010709553.1"
                     /transl_table=11
                     /translation="MIEKGNGRGQAVLGHHLVQSFSPEDDLTPEEIHGIGRQTVLEFTG
                     GEYEKEENLGKIEK"
     gene            complement(7386..7559)
                     /locus_tag="WOK_RS21985"
                     /old_locus_tag="WOK_01951"
     CDS             complement(7386..7559)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="WOK_RS21985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="WOK_01951"
                     /product="hypothetical protein"
                     /protein_id="WP_010709555.1"
                     /transl_table=11
                     /translation="MVYTKHFTIHTMDHLNQSKEYVQKASKTTIDKTSLGNTHYEQLFP
                     YIMNADKTLSKQ"
     misc_feature    7666..7679
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: strong, score: 23.98"
                     /tool="antismash"
     gene            <7730..8324
                     /locus_tag="WOK_RS13990"
                     /pseudo=""
     CDS             <7730..8324
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175178.1"
                     /locus_tag="WOK_RS13990"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="IS30 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="EEMPFNGKRKSNNHCEKRGQYAFHRDLRERAECYPDFETEFGHLE
                     GDTGDNIVGKGCKSAVITLVERLSKAIITLKVTGRKASDIEQSIDNWMSHFSSISFNLS
                     SLIVVKNFQIGNHYQTIMISLFSLRIQAVQGNVD"
     gene            complement(8408..9298)
                     /locus_tag="WOK_RS13995"
                     /old_locus_tag="WOK_01952"
     CDS             complement(8408..9298)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1108: ROK family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336226.1"
                     /locus_tag="WOK_RS13995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WOK_01952"
                     /product="ROK family protein"
                     /protein_id="WP_010709556.1"
                     /transl_table=11
                     /translation="MKKIIGIDIGGTTIKADVYDNMGKSLRDYREVDSHIDYENQSNAI
                     LEQVCQLVGEFQANHDIDGVGISSAGVVDSQKGEIVYAGYTIPGYIGTNFTETVKKRFH
                     LPVAVENDANCAALGEVWLGNAKGLSNVVMITVGTGIGGSVILDGHIVTGHHFTAGEVG
                     YLPIKGQDWQNLASTNALVKLYEEKSGKKKQTGHSFFADIEKRDPIAKETLDIFVDHLT
                     DGILTISYLLNPEALVLGGGIFTKPALLLSKIRNSLDSKVRDSRFLPKHIVAASLGNAA
                     GRIGAVKHFINQHLL"
ORIGIN
        1 tacggaaaaa actttttcct tcgttttaaa aggagacacc ccattataga gaagaggtaa
       61 ttctacattt tcataaactg tgttactctc aatcaaacta aagttttgga aaataaaacc
      121 tactgtttga tttctgattt tagaaagaat gtcatctgct gtattaacca aagatctgcc
      181 ttcaaacaag tattctccct caaatttacg atcgataaat cccaaaagat taattaatgt
      241 cgatttgcca gatcctgaag ggcccatgat cgctatcatt tccccatttt tgatggataa
      301 atgaatatct tttaatacat gaagtgactc ctcttcattt gcatagtatt tatttatctt
      361 attgagttca atcattgttt caccgtcact ttttggccat ctttcagact agtatctggg
      421 ttttcaataa ttgaatcatt ttctttaagt ccagtcttta ctacgtattt atcattatct
      481 tcttttactt ctattttctg tttaacgact tttccatctc tataaataaa aacgaatacc
      541 tcattattat tttctttaac tgtattcttt ttagccaact ctaaattatt cattggaaca
      601 ctgatttgga cattataacc ataatgaata ggttctttag ggctaattat aaaggagaat
      661 gttgaaattg tactattttt agttgatgat gtattttgtg gactgacagc catctcttca
      721 ggcagtgcgt taatttcagt aattattccg tctgttgctt tttcttcatt gatttgacta
      781 atcgttacag gttgacccac ttttacttta ttataatcat attctgtgac agaacctttt
      841 ataacagtct ctggactcac aatagtcgca tatggtactg ttgcgtctac tttcccttta
      901 tcgttaatat agacaattcc tttgaatggt gctgttacgg tagtggtgat tttctttttt
      961 gtttgttcaa tagtattatt ggcactggat aaatcaacat tcgccgcatc taacgcttgt
     1021 tttgcttgta aaaccgcctc tttttgtgaa tctaacgctt gttgtatggt ttcaatttta
     1081 ctatcagatt cagttttttc ggcttttttc atttcatcat ccattttttt atttacaatg
     1141 gtggcttgtt catttttagc aatggttaag cgattttcta attcttgttg tttttgtgtt
     1201 gcattatcta aatttatttg agcatttgta actgccagat tcaacttttc tagagattga
     1261 gtttgttctt ccgcttgatc ttcaacagtt gtattttgat aagtagccac tacatcattt
     1321 tcattgattt cttgaccatt tttcacggaa atagtattaa ttttcccaag ggattgatca
     1381 aaatttaaat aactagttgt tttaggttga acaattcctt ttaaaaccaa gggatcactc
     1441 ttttttaact tgataatttg ataggcctct tcttcttttg gttcagtggc gttcaactta
     1501 taaataaggg tcgcgctgat taataatact ccaacaagac agcctataat cgttaaagaa
     1561 atttgttgtt ttttagttaa ctttttcaat atcattctcc cctgtattta ctttgaatag
     1621 gtaaactaaa accataatta tcggtagttt gaacactaat gtgaaaatag tagttgtaat
     1681 tccgataatt agtcccatag attgcgtttc tgaagtattt gtaaaactag gtgttgttaa
     1741 taaagaaaat ataatggaca cgacagtcca tcccaaattt aaataataag gatattttag
     1801 tggaatttga tcttttttta tctttctatg tgaaataaaa aataaaatca caaaagcaat
     1861 tgtaaaaaga acatttaaaa ctgtaaacac ttttactggc atactgttta attgattata
     1921 aatactcatc ccctctaatc ctaagtcctt gtagagtgtt ttatctggac ttaatgcggc
     1981 aggtagtcca ataattgaaa aaattgttcc aattgaagct aatacaagag taattaaatt
     2041 ccatattctt atttttttaa tatatttact atagatttcc attaatattc cccattcatt
     2101 ttgagtaaga atcattaata atttacttta aaattattaa tgatcctata tttattaata
     2161 gcagtactaa tttgccataa aacaaaaatt tagttgcttt tatatcttta gtattgttgt
     2221 aaaataacaa taaaaagact atcacttcaa tagtagaagt actaattgat agtggtactc
     2281 tgttaatatt tattatttga gctgcaaaaa tagcaagtag attaactaag cttattgaca
     2341 ataagagact tataaatata tcttgatcat aatttttttt aaatccaaga ataagtaaaa
     2401 caatttttaa aattatgaat gtaacaaaaa agataaataa gttaactatt acaaccaaaa
     2461 ctatcataaa taaattaaat ttttcatcag agatatttaa tagttttgat gattctgcaa
     2521 tcatttccaa taatttatta tcttttccta ttagagttaa aaacaaaaaa acatttgtta
     2581 caagatatgg tataaaaaat acaagtatca accagttatt tttctttgta ttcaatccgc
     2641 tcacctgact tatctaagtt tatagtaaaa taattatatt tgtattctaa ttctaattcg
     2701 ttatgtgata ctaaaacaac acaagttcca tctaaaatta gttgatgaca ctgttctaat
     2761 aaaatagttc tactctgttt atcaatactt gaaaatgttt catctaaaat aactaaatcc
     2821 caatgccctt tctcaaaaag caatgtgcac gctgctttca acaacatccc taacgaggca
     2881 ttttctgcca aagtagttag ttgtttttct tcattgtaaa gacttttgat ctgctctaat
     2941 ttttgtggag taagctctag accatgaatt gaaaaaaata actctatatt ttctattatt
     3001 gttaggtagt agtgacataa agactggtct gtaagaaata aatttatttt attgttatgt
     3061 ttaaaagtcc ccgttgaaaa attttctata tttcctaaac atcttaaaaa aacacttttt
     3121 ccacttccgt tacttcctct acaaaaatac atactgttca aaaactttat actgatatca
     3181 ctaaaaatat tcttttcttt atacatttta ccaacgtttt ctaattcaat aactgatttc
     3241 accctatcat cccttctatt attcctgaaa tgcaaatcaa catatagctg aacacatatg
     3301 tttttttatt ttgtttaata aagtcgagaa catcgattat actaaaattt ttaaaaaata
     3361 tagtacaaca gtttaaagat aaaaacatat acaaaaacat gttaggtagt tctataattc
     3421 cgtgtttata caataattga aatgtctcaa atccacctac attgttaaca gaaatccacg
     3481 tttgaaaaga tatcatgaat aactcatata aaattagtaa aggtacaatt ggaaataaaa
     3541 gtatatattg caaaaaattt ttggtgttat taaaaataat tttcaacgca tacctaactc
     3601 cataatctat acttttaata ctaattccat taacttctga cggtatgtca aattgatcaa
     3661 atataaaata aactgacaca aggtaaagtg aagctattaa aaaaaatagg aaatatcctt
     3721 ttaaattaaa ttcattttct ttcttcattt ttatctacct tctataaaga tttaataata
     3781 tacgtttttg ccattaaaga taacagaata aagataatta gaatatattg aacaactaaa
     3841 ttattttctt ttttccttgt gaatattcct ttatttttta atttatagta taaaaaacta
     3901 atgcagaaaa ttactatgga taaatatata aaattaatca aaataactgt caactttcga
     3961 tccatgctaa aaattaaaga aaccatacct atcattacaa aaattaaaga cttatggaaa
     4021 taattcgata aaaaatatgt tttatttttc tgaactggtg aattacttaa aaaataaaat
     4081 tctgcactgg ttttactcaa atatttaaaa tgcctgtctg aaagtaattc catatctatt
     4141 aaactaaaaa aaatcaacaa accagtgtta tcgaaaataa aattaaaaaa actggaatac
     4201 ataattaaca aacaacaaat aaatagatat tcaaagtatt ctttaataga aactctactc
     4261 aaataagtta taaataactg attatacata tttttagaag taaaaatttt tgacatagtt
     4321 attttggcat tattcctaac atatttaaga tcaaaacttt tgaattttct ccttacaaaa
     4381 acatcaaaat acacaaataa taacaggaaa attactaata aaaatgaaag aactgaagtt
     4441 ataaatcctt tactaaacag taaacctagc aataagataa taagaataat tgatgtaaaa
     4501 atcttataca cacgttttgc taatagttgt attaaaaaaa gtattaattg taaaaagaat
     4561 gctgtaagta tgttgattac taaaagtcca ttagttatat ttaatattat attaaataaa
     4621 tttattaaga aaaccattga aaaaaaggga ttgtttttat taatcaagaa aagtatatat
     4681 ttgataaata ccttatctga gtaaaaatag ttataacttg tcatttgatt taactctaac
     4741 tctatttttg gcaaattata gtttaacagc aaaaaattta ttaagataat aggaagtaac
     4801 aaattattta aattgattat taataataat aacgtacacg tacacacata catgattaaa
     4861 aaaaataatt taaaaatgtg aaagttttct acaatcctta attttctttt gaaaattttc
     4921 aaataaatat ttcccattgg ttattacctc ctatattatt aacggacagc tttaaaagct
     4981 gtccgttaat aatataattt ttaccatgca gcagcatatg cttttccata tttttttacc
     5041 aaagcttttg ctgtagctag aactgctaca ccaataccac ctccaccaac aacagctgca
     5101 attattccta ctacagccgc aacagaacta gcagcagaaa taatatcaat tactttttta
     5161 gctgcataag ctgaaattcc taatgttgat gttaaatgtg gttgagtcag actaattgca
     5221 aaagctacta atacaaaagc tacacccata tacatttttt tattaaataa tacattattt
     5281 aacttcattt tttcacctcc tctctttgtt tatctatcca tgtgcaaaac gtttgatatc
     5341 tagtatttat acgcgtttca gaaacatgga tttttacttt ggtactcaaa tttatagaag
     5401 caattatctc taaaatggta aaactagttt gctagactta taaaggaact gattcgtatc
     5461 taccgtcaag aaattcttga aaatataact ctttttattt cacaacctgt cgattactaa
     5521 cagaaattga aacacggtta gaagtaattt aactaccctt atttagtata tttaactaac
     5581 tattcttcac caatctatat tccttaaata atacatattt atccttatat ggtatgcttt
     5641 ggatgtatat taataaagta gacaaaattt ctgctatact actatttaat tagacactat
     5701 tttgaaagga ctgtttttat gatagacaaa cgtaaatctc ctcgtaagtt tgatgaagag
     5761 tttaaaaatt ccatcgttaa actttacgaa aatgacaaat ctcaaaattc tttagccaaa
     5821 gaatatggat tggctgtttc cattatcgtt cgttggataa agcaattctc tgaagttaaa
     5881 tttgatgatg gctcgatcct aacggctcgt caaatccaac aactccaaaa aagaaatatg
     5941 caactttaag aggaaaactt aatcttaaaa aaaaaagcga ttgccatatt cattccacac
     6001 tcgatgaacg actaaaagcc atttattttc ttcgacacga acatagaatt agtacacttt
     6061 gtcgtgttct caaggttaat cgatcgactt actataaatg agtttttgca aggccagctc
     6121 ctcgaacatt ggaaaactaa caattgagaa agaagattct agagatttat accgctactc
     6181 aaaggagaat tggtgctgca aaaatccaaa ggattttatc gcgtgattat ggcgtctcca
     6241 ttagtgttgg acgggtttat cgattaatga agtcgataac tcttcctaaa atgtctacta
     6301 gaaaacctgc ttggttctgt tgcaaagatt ttcaaatatc ctgatgtagg ggaaaattaa
     6361 gagaaaaagc agtttggaga cttcaaatgg ctcattttga aggaaagcaa ttcaagcaag
     6421 atattataat ggtagccgtt ggctactatt tgagatataa cttgagttat ctagccattt
     6481 ctgaaattat gagtgatcgt ggggtcatgg tttgtcatac aaccatttat cgatgggggc
     6541 aagaataaaa aatcccacct gttcttttgt cttttgccag tcaatagaat caaacgttgg
     6601 caatgtatag tcactcacgt caaaaatcct cacaactttc accttcttta tgttataata
     6661 tgaaagttgc taagagattt ttcttttagt gatatgagga tggccaggac agttatcctg
     6721 gctttttcta tttcttcaac aagcgattct ctttttcgtt ggtaagtaca tttccttgtt
     6781 catcccattc atgatctact ttcgcttttg aacgaacata ttgcgctaaa tgtgaatcat
     6841 gtgggacata tcgaaaccgc tcaacatcaa acaaatcaat cgctgcttgg ttgccatttg
     6901 cgtacgtaaa ttgtaatgat tcatcctctg aaactaaatt ttttacataa gataatgcaa
     6961 ccacttgttc aaaactttcc agttcaaatc gatcgtcctc atccattgaa ttccacgcaa
     7021 cgactaaatc ttttgttccc attcctattg tcctcctcat aaaatatatt gcttactttt
     7081 ctattttccc tagattttct tccttttcat attcaccacc tgtaaattcc aaaacagttt
     7141 gacgaccaat tccatgaatc tcttctggcg tcaaatcgtc ttcaggagaa aaggattgaa
     7201 ccaaatggtg acctaaaact gcttgtcctc gtccattacc tttttcaatc atctcctatc
     7261 aaaaaccatc gtttctgttt cagaatcaaa catcaaatac gtgccttttt gtttgcctac
     7321 tcgttctttc gtgtaaagaa attcatttgc tgcattgatc atatcggtta ttccatgtcc
     7381 tgaaactact gcttacttaa tgttttatcc gcattcatga tatacggaaa cagttgttca
     7441 taatgcgtat ttcctaacga tgttttatca atcgtcgtct tacttgcttt ttgaacatac
     7501 tcttttgatt gattcaaatg atccatggta tgaatcgtaa aatgttttgt ataaaccatc
     7561 gttacaccgt ccgttctttg cttaattctt cttttaattt ttcggtaacg agcgtcttta
     7621 cttcgtgcat catttctaat aattgttgga catcttcgtt agatccaaac gttcgtttca
     7681 accgtccacg attatgtttg ttatagtctt ctacagtatt ttcaatcctg aagaaatgcc
     7741 ttttaatggt aaacgaaagt caaataatca ttgtgaaaag cgaggacaat atgctttcca
     7801 tcgagatctg cgcgagcgcg cagaatgtta ccctgatttc gagacagaat ttggtcattt
     7861 agaaggagac actggagaca atattgttgg aaaagggtgc aagagcgctg ttattacctt
     7921 agtagaacgt ctgtctaaag ccatcatcac cttgaaggtt actggacgta aggctagcga
     7981 tattgagcaa tctattgata attggatgag tcacttttca agcatctctt tcaatctatc
     8041 atctttgatt gtggtaaaga attttcagat tggaaatcac tatcaaacca tcatgatatc
     8101 gctattttct ttgcggatcc aggctgtcca aggtaacgtg gattaaatga acaatcaaac
     8161 tgcttactaa ggaaacatgg tcttccaaag aatatggatc ttagtggtct ttctcaagaa
     8221 ttcttatcag tgatagaaga aacaggattc caagaaagtc attgaattac caaaccccat
     8281 accaagtttt tctaactaac atgaaaatat ctaacttaat ttgacaattt ggggcctgta
     8341 acagctgatt aagtttaagc ttggttttta gtctagcttg tttaattagt tgattgtact
     8401 cttcgactta taacagatgt tgattgatga aatgcttaac agctccgatg cggcccgccg
     8461 catttcctag tgaggctgca acgatatgtt ttggtaagaa gcgactgtcc cgtaccttgc
     8521 tatcgagtga atttcgtatt ttagagagta acaaggctgg ttttgtgaat atcccgccac
     8581 ctagaaccaa ggcctctgga tttaacagat aggaaatcgt taaaatacca tctgttaaat
     8641 gatctacaaa aatatccaag gtctctttag caatcggatc tctcttctca atgtccgcaa
     8701 aaaagctatg accggtttgt ttcttcttac cactcttttc ctcatacaac ttgaccaggg
     8761 cattcgtaga tgctaaattt tgccaatctt gccccttaat cggtagataa ccaacttctc
     8821 ccgctgtaaa atgatgccca gtcacaatat gaccatcaag aataacactg ccaccaatcc
     8881 ctgttccgac agtaatcatg acaacattcg atagtccttt agcatttcct agccatactt
     8941 cacctagagc agcacagttc gcgtcatttt ctactgctac cggtaaatga aatcgcttct
     9001 tgactgtttc cgtaaaattt gtaccgatat aacctggtat cgtgtagcct gcatagacaa
     9061 tctctccctt ctgactatct acaacccctg ccgacgaaat accgacaccg tcaatatcat
     9121 gatttgcctg gaattctcca accagttgac acacttgctc taaaatagca ttggactggt
     9181 tctcgtaatc aatatgggaa tcaacttctc tataatcacg aagactcttc cccatgttat
     9241 cgtaaacatc agccttaatt gtagtaccgc caatatcaat tccgattatt tttttcatag
     9301 gtgttatcct ttccattcat ccgttatatc atctactaaa aagaagattt tagacgctta
     9361 ttaaaaatgg gattgggaat gcccccaatc ccatatacat acttattgac tagttactct
     9421 ttaaggatat aactatttga taaggaggtt agactagcca ttagtaattt cagaaagtgc
     9481 tactttgcga tcttcaataa cagataaggt acatgcgtct gcagtcgcaa tcgattcaag
     9541 agctgctaca ccattcaaca atttagagta ttcctctgta atctcactcc tttgatgtaa
     9601 tctcactcct ttgatgatat cgtgcaagta agccatttct ttatcgatac aggtttgtaa
     9661 ccatagtggg caacgaacgc ctggcttacc ataagcaatc gcaccatcca taccgcgtcc
     9721 ggtatagatt gccgttctat cgtcatcttc ttcttgcgtt tcgtgtacta agaaatgaga
     9781 ctctccttcg cctttaacac gcagtgtacc acctgtgttg tacaagtcca atttaatagc
     9841 tccttcagta ccttcaatca agacgtaatg ttcaccccaa cggaaggcat taccatattc
     9901 caatacggca taacggtcat ctgagtattc caaattaaca attagcatgt cgtcttcatc
     9961 accaaagtta tcacctttat gatagacatt acctcctacc atcgttactt tttctggcat
    10021 tcctcccatg ataaactgga tacaatccaa ctcatgaata tgatgataca agtgtcctcc
    10081 agattgtgaa cgaagtttct tccaagatac ggttggttgt tgttcttccc aacctgtgcg
    10141 tgctgcatgg cagtataaaa ctttaccaat cttgccttgt gtgatcagtt ctttggcatg
    10201 atggacacca ttaaagaagt tcataatatg tccagccata aaaataacat tattttcgtc
    10261 acaggcatct accatagcct tacagtct
//
