LOCUS       NZ_KB944666            11044 bp    DNA     linear   CON 09-DEC-2024
DEFINITION  Enterococcus faecalis EnGen0336 strain T5 acAro-supercont1.1, whole
            genome shotgun sequence.
ACCESSION   NZ_KB944666
VERSION     NZ_KB944666.1
KEYWORDS    WGS; RefSeq.
SOURCE      Enterococcus faecalis EnGen0336
  ORGANISM  Enterococcus faecalis EnGen0336
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2806553)
  AUTHORS   Earl,A.M., Gilmore,M.S., Lebreton,F., Walker,B., Young,S.K.,
            Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A.,
            Alvarado,L., Arachchi,H.M., Berlin,A.M., Chapman,S.B., Dewar,J.,
            Goldberg,J., Griggs,A., Gujja,S., Hansen,M., Howarth,C.,
            Imamovic,A., Larimer,J., McCowan,C., Murphy,C., Neiman,D.,
            Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P.,
            Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     The Genome Sequence of Enterococcus faecalis T5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2806553)
  AUTHORS   Earl,A.M., Gilmore,M.S., Lebreton,F., Walker,B., Young,S.K.,
            Zeng,Q., Gargeya,S., Fitzgerald,M., Haas,B., Abouelleil,A.,
            Alvarado,L., Arachchi,H.M., Berlin,A.M., Chapman,S.B., Dewar,J.,
            Goldberg,J., Griggs,A., Gujja,S., Hansen,M., Howarth,C.,
            Imamovic,A., Larimer,J., McCowan,C., Murphy,C., Neiman,D.,
            Pearson,M., Priest,M., Roberts,A., Saif,S., Shea,T., Sisk,P.,
            Sykes,S., Wortman,J., Nusbaum,C. and Birren,B.
  CONSRTM   The Broad Institute Genome Sequencing Platform, The Broad Institute
            Genome Sequencing Center for Infectious Disease
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2013) Broad Institute of MIT and Harvard, 7
            Cambridge Center, Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: allpaths v. R42411
            Assembly Name                     :: Ente_faec_T5_V1
            Genome Coverage                   :: 289.0x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000393015.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 13:32:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,757
            CDSs (total)                      :: 2,690
            Genes (coding)                    :: 2,666
            CDSs (with protein)               :: 2,666
            Genes (RNA)                       :: 67
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 8 of 24
            Pseudo Genes (incomplete)         :: 14 of 24
            Pseudo Genes (internal stop)      :: 4 of 24
            Pseudo Genes (multiple problems)  :: 2 of 24
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:17:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 814353
            Orig. end                         :: 825397
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB944666.1.
            Please be aware that the annotation is done automatically with
            little or no manual curation.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11044
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11044
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11044
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6044](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6044
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            617..817
                     /db_xref="GeneID:60893071"
                     /locus_tag="WMS_RS06915"
                     /old_locus_tag="WMS_00757"
     CDS             617..817
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:60893071"
                     /gene_functions="regulatory (smcogs) SMCOG1255: cold-shock
                     DNA-binding domain protein"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /locus_tag="WMS_RS06915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WMS_00757"
                     /product="cold-shock protein"
                     /protein_id="WP_002355647.1"
                     /transl_table=11
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQSDGFKTLDEGQ
                     AVTFDTEEGPRGLQATNIVKA"
     misc_feature    1319..1332
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 20.02"
                     /tool="antismash"
     gene            2275..2478
                     /db_xref="GeneID:60893072"
                     /locus_tag="WMS_RS06920"
                     /old_locus_tag="WMS_00759"
     CDS             2275..2478
                     /codon_start=1
                     /db_xref="GeneID:60893072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405754.1"
                     /locus_tag="WMS_RS06920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WMS_00759"
                     /product="hypothetical protein"
                     /protein_id="WP_002410046.1"
                     /transl_table=11
                     /translation="MNVEEVLVSQKTPNAINEETELYGFGYGSVVENITVSKGGGGGGG
                     GGGGGCCCCCCCCCGGATGLEE"
     misc_feature    2491..2502
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            2543..3373
                     /db_xref="GeneID:60893073"
                     /locus_tag="WMS_RS06925"
                     /old_locus_tag="WMS_00760"
     CDS             2543..3373
                     /codon_start=1
                     /db_xref="GeneID:60893073"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04424"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379875.1"
                     /locus_tag="WMS_RS06925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WMS_00760"
                     /product="McbB family protein"
                     /protein_id="WP_010819226.1"
                     /sec_met_domain="TIGR04424 (E-value: 4.4e-88, bitscore:
                     292.8, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYKVNPFIWHDLSEKETFFQTKLSSIIITNKNLVRMLKDLEKNEV
                     INFEESLLSNYFRSNMINNVLIFLEENKLIKKLDIENNKVNEVLVYTDDSEFSELFDYV
                     FSEKFTISHQSSISHLESKNMDGKLLIVFLKAFNFKEYENIVNIAKEKNVVIKIIFSYN
                     HHIYFSNFYKREWYNPCPICFFSELESQLRGEITSDTANFQTIIDLLYTKSQNFAIEYP
                     LQKDDYFTIVYTIMRNLDSDLMKCDLDEVLSIDLNNHLVSKDIAYHWGYCDCYE"
     gene            3366..4022
                     /db_xref="GeneID:60893074"
                     /locus_tag="WMS_RS06930"
                     /old_locus_tag="WMS_00761"
     CDS             3366..4022
                     /codon_start=1
                     /db_xref="GeneID:60893074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401933.1"
                     /locus_tag="WMS_RS06930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WMS_00761"
                     /product="hypothetical protein"
                     /protein_id="WP_010819227.1"
                     /transl_table=11
                     /translation="MSDYYWNEYKNVLLDEAYLIDTLKYQQRVVNKQVEENNLDELSDV
                     LGLYNVKYSDIRLAIYPFDELPVFIKTLVSDLQKNYASFIYKVSMPILAITKKIGNVNT
                     GCYSFDFKNNFLILYETDVTKIREIVNDKLVLAFFLNIEDAICLYKKKAYTNGIKEIGY
                     VSKSSQLAISKEKNIIINEMLVPEQQLTNGLGINLRKCLLMETLILEVLTNDIHE"
     gene            4006..4983
                     /db_xref="GeneID:60893075"
                     /locus_tag="WMS_RS06935"
                     /old_locus_tag="WMS_00762"
     CDS             4006..4983
                     /codon_start=1
                     /db_xref="GeneID:60893075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365263.1"
                     /locus_tag="WMS_RS06935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WMS_00762"
                     /product="hypothetical protein"
                     /protein_id="WP_002394300.1"
                     /transl_table=11
                     /translation="MIFTNKSSYGVKSDMFLSNINIGYVNFSRVKIKQGTTAGSAFSVD
                     ENKQLSLIKSYNEYLERLFLGIPLSTNKTISSVNLEKNIVSSRKYSEFGYGNHEFGFND
                     TTGTSSGKLSEVIIPKALTELIEKNEVFCFWYGLKGEKLKQDKEIHDLINDYNFISNMF
                     KIYVVRELSNYSTVIVMGFLEGKLLTSGVCCSITMEQSLILALKEAKGIEWQTFNNPLA
                     KANKFSDKLHKQILKKVKFMDETYKVITKDSIQATKYIETSDWINHVEISVIGDDINRG
                     VKTIKCISKQLLNSVPIKINIERQKDKEIIKRYLIDYSIDCPIQ"
     gene            5001..6044
                     /db_xref="GeneID:60893076"
                     /locus_tag="WMS_RS06940"
                     /old_locus_tag="WMS_00763"
     CDS             5001..6044
                     /codon_start=1
                     /db_xref="GeneID:60893076"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401935.1"
                     /locus_tag="WMS_RS06940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WMS_00763"
                     /product="YcaO-like family protein"
                     /protein_id="WP_010714216.1"
                     /sec_met_domain="YcaO (E-value: 5e-07, bitscore: 27.0,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKGFLRNKVNFVLNEDLFMSHHYNVYIEKYSKRPIKAYEVDLKNK
                     RQHILSTLGEYLERETLINENSPKEHTVIATNLIDGSIKQVNLDMILFKNSFVDSSGMA
                     SHKKSKPLIWTAYKEFFERQSYITNFLFHTKAQQLNIDKIEEIKKIDRYLTNYLDNVKY
                     YNISLSKNLYVVLAVGYGEKYKAVGLGTSQSARKAVIKSQKEILQYFATSHSKYKEGKV
                     SQGSGLVIDNKDIYHDYFDRLSSKEVKDLYAYLEGSEFVILNNEEKTEKVSHTFTIKNI
                     SNSLNMSPYITMFENNHAKNVKVVKVFDPKWFPNMAPRNFSKEIILNIENILKLKRKNF
                     SELLPFP"
     gene            6057..6737
                     /db_xref="GeneID:60893077"
                     /locus_tag="WMS_RS06945"
                     /old_locus_tag="WMS_00764"
     CDS             6057..6737
                     /codon_start=1
                     /db_xref="GeneID:60893077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405757.1"
                     /locus_tag="WMS_RS06945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WMS_00764"
                     /product="hypothetical protein"
                     /protein_id="WP_002365261.1"
                     /transl_table=11
                     /translation="MFISVKSYALFNIKMLLKDKIPLLWSIALPVITFFMNYRSVTNEW
                     ELAYWWVYIVVCSYVYGVGVYALQLKEYGSLKTIFSINNSPWAFFLGNLLTQIMFSFIS
                     VNLLNLIVVILLPLSFLRLFLYSFITIIACLPVAFFSYNLTLIKKVHANTINSIFTMLI
                     FGLFILLNTNIELNKYNPLSILSNVLIEHKNMDVFKYVTFSIFLVFISMYSIVKFEPIS
                     NERR"
     gene            6724..7314
                     /db_xref="GeneID:60893078"
                     /locus_tag="WMS_RS06950"
                     /old_locus_tag="WMS_00765"
     CDS             6724..7314
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:60893078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002394296.1"
                     /locus_tag="WMS_RS06950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WMS_00765"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_002394296.1"
                     /transl_table=11
                     /translation="MKEGKAMIEIKDLNIPYVDKKISMNILKEYNLFTGDNGNGKTLLL
                     DYISGVKKAKRTVIKGNESIIYINQNIYFSDRLSGMDFLKFSYGLDGKKSIQSFYQLSD
                     KILKREDIDKLLKKQWGMLSGGEKKFLYALILMSLEREWYILDEPFAFVDKKRKKMLYK
                     IIDIKISEDKGIILTTHEEDEVVLKSVSKVFEI"
     gene            complement(7670..8110)
                     /db_xref="GeneID:60893079"
                     /locus_tag="WMS_RS06955"
                     /old_locus_tag="WMS_00766"
     CDS             complement(7670..8110)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:60893079"
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002394295.1"
                     /locus_tag="WMS_RS06955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WMS_00766"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_002394295.1"
                     /transl_table=11
                     /translation="MTAYSIGEISKMFNIPVSTLRYYDKEGLFPEITRVSGIRKFSNNE
                     IEALRVIECLKKSGLEIKDIKQFMKWTKLGSETFKIRKELFERQKVSVEKELRQMEKVL
                     DMIKFKCWYYDEVLKQGNEVAVKSKIPNNLPKEIKLYYENSH"
     gene            8193..9197
                     /db_xref="GeneID:60893080"
                     /locus_tag="WMS_RS06960"
                     /old_locus_tag="WMS_00767"
     CDS             8193..9197
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:60893080"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365249.1"
                     /locus_tag="WMS_RS06960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WMS_00767"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_002394294.1"
                     /sec_met_domain="PF00561 (E-value: 2.9e-05, bitscore: 21.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTIGGKARMLEDTTMIQKWDKVFQKSDKVNHRNVTFTNRFGIAL
                     VADLYEPKRQAEEFPAIAVCGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGI
                     PRNVASGDINTEDFQAAVDFLSVQKNVAQDKIGIIGICGWGGMALNAAAIDTRVKATVV
                     STMYDMSRVTANGYFDSIDVDGRYEMRQQLNAQRIEDFKNGDYARSGGVPDSLSESAPY
                     YIKDYFDYYKTDRGYHKRSLNSNEGWNMTSALSLLNTKFLSYSDEIFSAVLIIHGEKAH
                     SRYFAEDAFKKLQGDNKELLIIPKATHTDLYDKIDIIPFDKMTEFFYQNLEVR"
     gene            9211..9960
                     /db_xref="GeneID:60893081"
                     /locus_tag="WMS_RS06965"
                     /old_locus_tag="WMS_00768"
     CDS             9211..9960
                     /GO_function="GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:60893081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365247.1"
                     /locus_tag="WMS_RS06965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WMS_00768"
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="WP_010819229.1"
                     /transl_table=11
                     /translation="MIEKQTAGRDFLGEFAPKFAELNDDVLFGQVWAREDKLSLKMRSI
                     VTITALISKGVIDSSLNYHLETARKNGVTKTEMAELLTHLSFYAGWPNAWAAFRLAKEV
                     YVEDHNKEEHGGLFGQGELNTAYSEYFIGQSYLKPLTDPKETVFIANVTFEPACRNNWH
                     IHHAKSGGGQILICVDGEGWYQEEGKEAQSLKPGDTITIPANVKHWHGAKKDRWFSHLA
                     IEVPGVETSNEWLEPVKNEFYDSLKEC"
     gene            9963..10547
                     /db_xref="GeneID:60893082"
                     /locus_tag="WMS_RS06970"
                     /old_locus_tag="WMS_00769"
     CDS             9963..10547
                     /codon_start=1
                     /db_xref="GeneID:60893082"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1156: Chloramphenicol acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002365246.1"
                     /locus_tag="WMS_RS06970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WMS_00769"
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="WP_002365246.1"
                     /transl_table=11
                     /translation="MKVSIEDVINGYRSDAPVDLERDAYAEELYQEAIKIGIEMNTKYH
                     SEEELREIMSYLIGKKVDETFRIFPPFYTDFGKNITLGKNVFINSGTHFQDQGGIVIGD
                     GVFIGHNVVLATINHDLFPKNKRKNHYAPIVLKNNVWIGSNATITSGVTIGEWSVVAAG
                     AVVTKDVPPYTVVGGVPARVLKSIDKEENGR"
ORIGIN
        1 ttcttaaaaa tcaatctgat gaaattctta tcttaaaaga tcggccagac acatggaatt
       61 tccctggcgg aaaacaagaa cttggtgaat cagcgctgga atgtgcgatt cgtgaaatga
      121 aagaagagtt agcagtggaa attactgaac ttgaagaaat ttttactgga ccacttgttt
      181 ttgattcaat cgtttggcaa ggacattttt attttgccaa acaagcccaa ggaaaagtta
      241 ccctgaacga accaaataaa attaaaggcg tacaatttat taagcggttt gaggaagtaa
      301 atttttcacc aggattagct ccgctatttg attatttggt gcgtagcgat tatttaaaat
      361 taaacaaaac taactggaaa tagagagaat aggagtgtct tttgctaggg ctttgtcaag
      421 cgcatctttg attagtttag aaagcccaag gaaaagtcgt gggcattgtt tattcctaat
      481 aaatgtgtta tagtaattaa gtaatcaatt ttgataagta atttgagtta tatatttaaa
      541 aactatactt ctccttttgc cactcaacat tttttgcaat aacttgcata tttttatgca
      601 ctaggaggaa atatatatga acaacggtac agtaaaatgg tttaacgcag acaaaggttt
      661 tggatttatt acaggtgaag atggcaatga tgtatttgca catttttcag ctattcaatc
      721 agatggattt aaaactttag acgaaggcca agcagtaact tttgatactg aagaaggtcc
      781 acgcggctta caagcaacga acatcgtaaa agcttaataa tgaatgaaaa cggaccacgt
      841 gttggtccgt ttttttgata gctgttgtct gatctcgcca ttaattacat agcaaaacaa
      901 agcagaggcc atttaaagga ggagaatctg aagatgacct taactgagct gttacttgta
      961 agtggattat tttggctagt aatcataatt ggtgtgattg cgggtagcat atttctaatt
     1021 aagtatttta gaggtcggag ctgaacggtt ctgttctcgc caattatcag gttttaagcc
     1081 tgggacaaaa atccaaagtg atttttgccc caggttcttt atttaattag ggtatcaagt
     1141 tttaggtaaa ataattaaca aattaaaagc aatttactat ataatcagaa agggatcttt
     1201 aagagagaag caggtgtttg cttgtcgtaa tactttgttt agcagataga aaaaggaaac
     1261 gttatgcgtt acaataggaa cggaggagtt ttaaccttat tggatttgta gctgaagcag
     1321 aacaaatgtt gtcgcgttaa gtgcgattga gcttgccaca agcaacattt aaaaaaacta
     1381 cggatagaaa aatcattcct aaacaagaaa aaaacactct ttagatgaat aaagagtgtc
     1441 cagaaacgtt gataaatcaa catatttaaa taacgcgccc tgagggaatc gaacccccgt
     1501 ctcaagaacc ggaattttat tgatagtttt tgaggagaga ggtagtgttg atatataagt
     1561 aattgatact tttgatagct gtaataaatt ttataaaatt ttatggaatg tttaagggtc
     1621 gtccttaaaa gtcgtcccta ttttggaaga gaagactaca taattaaata atgctactta
     1681 acttcaataa tattcttttg ccaagttcaa aatactatct gttttaattg gagttataag
     1741 tgaaatatgc agaaattatg atttcaggta gtagtttctg tttaaatgta ttgagaatac
     1801 tagtttttaa tgtctttatt tgcatagtaa ataagttcaa aaagcaatga ttgttagctc
     1861 ttgtgttaac ggttgttgct ttttaaatac ttaaatatgt tttgttatta aacaatatgc
     1921 tttccttata gtaagtatca attcataaaa ctaggtattc acaattgtta ttaaagaggt
     1981 aaaatccgaa aatatgcgaa tatgcattgt atattataat tttaatgtga taaaataaat
     2041 attttaaaaa ttatttttgt tagaattttc attgatataa aaatagaaaa attaatgata
     2101 gttgttttgt tattaatttt tttgtttttg ttgtctaatc aaagattttt agatttttca
     2161 gtataaaaat acgtttttta attagaataa gatgagtttt tgcttgaaaa atgattggat
     2221 agtggtaaaa taatttcatc aatattgata aaattataat ggaggtgtat taaaatgaat
     2281 gttgaagaag ttttagtgtc gcaaaaaacc ccaaatgcaa ttaatgaaga gactgaatta
     2341 tatgggtttg ggtatggttc tgtcgttgag aacattactg tctctaaagg tggcggcggt
     2401 ggtggaggcg gcggtggcgg tggctgctgt tgttgttgct gctgttgttg tgggggtgcc
     2461 acaggattag aagaatagaa acttcataag gtaagtggac gcacttacct ctttacataa
     2521 tcaattaaaa aggagtgaaa aaatgtataa agtaaaccct tttatatggc atgatcttag
     2581 tgaaaaagaa acattctttc aaacaaaact atcttcgata attattacta ataaaaattt
     2641 agttagaatg ttaaaagatt tagaaaaaaa tgaagtaata aattttgagg aatctctatt
     2701 atccaattat tttaggagca acatgattaa taatgtactg atctttttag aagaaaataa
     2761 attaattaaa aagctagata tagaaaataa taaggttaat gaggttttgg tatataccga
     2821 tgattctgaa ttttctgaat tattcgatta cgtgttttcc gaaaaattta caattagtca
     2881 tcagagtagt atttcccact tagaatctaa aaatatggat ggtaaattgc ttatagtatt
     2941 tttaaaggct ttcaatttta aagaatatga aaatattgta aatatagcca aggaaaagaa
     3001 tgtagtcatt aaaataattt ttagctataa tcatcatatt tatttttcga atttctataa
     3061 aagggaatgg tacaatccat gtccaatatg ttttttttct gaactagaaa gccaattaag
     3121 aggagaaata acttctgata cagctaattt tcagacaatt atagatttac tttatacaaa
     3181 atcacaaaat tttgctatag aatacccgtt gcaaaaggat gattatttta cgatagttta
     3241 taccataatg aggaatttgg atagcgattt aatgaaatgt gacttagatg aagttttatc
     3301 tattgattta aataatcatt tagtttcaaa agatattgct tatcattggg ggtattgtga
     3361 ttgctatgag tgattattat tggaatgaat ataagaatgt actattagat gaagcatatt
     3421 tgattgatac gttaaaatat caacaaaggg tagtgaataa acaagtagaa gaaaataatt
     3481 tagatgaatt gagcgatgtt ttaggtcttt ataatgtgaa atactcggat attaggttag
     3541 cgatatatcc ttttgatgag ttacctgtct ttataaaaac gttagttagt gatttacaaa
     3601 aaaattatgc ttcctttata tataaagtta gtatgccaat actagccata acaaaaaaaa
     3661 tagggaatgt aaatactggt tgttattctt ttgattttaa aaataatttt ttgattctat
     3721 atgaaactga tgtaactaag attagagaaa tagtaaatga taaactggtg ttagcttttt
     3781 ttttaaacat agaggatgca atttgtttat ataaaaaaaa ggcgtacaca aatggtataa
     3841 aagaaattgg ttatgtttct aaaagcagtc aattagcgat tagtaaagaa aaaaacataa
     3901 taataaacga aatgctagta ccagagcaac aactgacaaa tggattaggt ataaacttga
     3961 gaaaatgttt gttaatggaa actttaattt tagaggtgtt aactaatgat attcacgaat
     4021 aaaagttcct atggtgtgaa aagtgatatg tttttatcaa atataaatat tggatatgtg
     4081 aatttttcac gtgtcaagat taagcaagga acgacagcag gtagtgcttt ctcagttgac
     4141 gaaaataaac agttatcact tattaaaagt tataatgaat atttagaaag acttttctta
     4201 gggataccat taagtacaaa taaaactatt tcttctgtaa atttagaaaa aaatattgta
     4261 agtagcagaa aatattcaga atttggatat ggaaatcatg agttcggatt taatgatacg
     4321 acaggaactt catctggaaa actttcagaa gtaataatcc caaaggcact aacagaattg
     4381 attgagaaaa atgaagtatt ttgtttttgg tatggtttga aaggggaaaa gttaaaacaa
     4441 gataaagaaa ttcatgactt aattaatgat tataatttta tttcaaatat gtttaaaatt
     4501 tatgtagttc gtgaacttag taattattca acagtgatcg tgatgggttt tttagaaggg
     4561 aaattactaa cctcaggtgt atgttgtagt attactatgg aacaatctct aattttggcc
     4621 ttgaaagaag ctaagggaat agagtggcaa acttttaata atcccttagc aaaagcaaat
     4681 aaattttctg ataaattgca taaacaaatt ttaaaaaaag taaagtttat ggatgaaaca
     4741 tacaaagtta ttacaaaaga ttctatacaa gctacgaaat acattgaaac ttctgattgg
     4801 attaaccatg ttgagatatc cgtaattgga gatgacataa atagaggagt taaaacaata
     4861 aaatgtatat ccaaacagtt actaaattct gtaccaatta agataaacat tgaaagacaa
     4921 aaagataaag agattattaa gagatattta attgattatt ctattgattg tcctatccaa
     4981 tgatagaaag gagaatactt ttgaaaggtt ttttaagaaa taaggtgaat tttgttttaa
     5041 atgaggattt atttatgtca catcactata acgtatatat agaaaaatat tctaaaagac
     5101 ctatcaaagc ctatgaggtt gatttaaaga ataaaaggca gcatattctt tcaactttag
     5161 gagagtattt ggagcgagaa acactcatca acgaaaattc accaaaagag catacggtta
     5221 ttgctacgaa tttaattgat ggaagcatta aacaagttaa tcttgatatg attttgttta
     5281 agaatagttt tgttgattct tctggtatgg cctcgcataa aaaatcaaag ccattgatat
     5341 ggacagcgta taaagaattc tttgaaagac agagctatat tactaacttc ttgtttcata
     5401 ctaaggctca gcaattaaat atagataaga tagaagaaat aaaaaaaata gaccgatatt
     5461 tgactaatta tttagacaat gtcaaatatt acaatatatc tttaagtaag aacctttacg
     5521 tagtacttgc cgttggatat ggtgaaaaat acaaagcagt gggtttggga actagtcaat
     5581 cagcaagaaa agcagtaata aaatcacaaa aagaaatatt acaatatttt gctacatcgc
     5641 attcaaaata taaagaagga aaagttagtc aaggctctgg attagtaatt gacaataagg
     5701 acatttatca tgactatttt gatagattga gctcgaaaga agttaaagat ttatatgcat
     5761 atcttgaagg tagtgaattt gttattctga ataatgaaga gaaaacagaa aaagtaagtc
     5821 atacatttac tatcaaaaat ataagcaatt ctcttaatat gtctccgtat attacaatgt
     5881 ttgaaaataa tcatgctaag aatgtgaaag tagtgaaggt atttgaccca aagtggtttc
     5941 ccaacatggc accaagaaat ttttcgaaag aaataatatt aaatatagag aatatattaa
     6001 aattaaaaag aaaaaatttt tcagaactgt taccatttcc gtaaggagga gtgaaaatgt
     6061 ttatctcagt taaatcatat gcattgttta atattaaaat gttactgaaa gataaaatcc
     6121 ctttattgtg gtcaatagcc ttacctgtaa ttactttttt tatgaattat agaagtgtaa
     6181 caaatgaatg ggaattggca tattggtggg tatatattgt tgtttgttcc tatgtttatg
     6241 gagtaggagt ttacgcatta caattaaagg aatatggaag cttaaaaaca attttttcaa
     6301 taaataattc accttgggca ttttttttag gcaatctatt gacgcaaatt atgtttagct
     6361 ttatcagtgt aaacttactt aatttaattg tggttatttt actaccatta tcgtttctcc
     6421 gtcttttttt atatagcttt attactatta tagcgtgttt accagttgca ttttttagtt
     6481 acaatttaac tctaataaaa aaagtgcatg ctaatactat aaattcaata tttacaatgc
     6541 taatttttgg tttatttata ttgttaaata cgaatataga gttaaataaa tataaccctc
     6601 tgtcaatttt atctaatgtt ttaattgaac ataaaaatat ggatgtcttc aaatatgtta
     6661 ctttttcaat atttttagtt tttattagta tgtattctat agttaaattt gaaccgattt
     6721 caaatgaaag aaggtaaagc tatgatcgaa ataaaggatt taaatattcc ttatgtagat
     6781 aaaaaaataa gtatgaatat tttaaaagag tataatttat ttactggtga taatggtaac
     6841 ggaaaaacct tactattaga ttacatttca ggagtaaaaa aggcaaaaag aactgtaatt
     6901 aaggggaatg aaagtatcat atatattaat caaaacatat atttttctga tcgtttatca
     6961 ggaatggact ttctgaaatt tagttatggt ttggacggaa aaaaaagtat tcaatctttt
     7021 tatcaattaa gcgataaaat tcttaagcgt gaagatattg ataagttgct taaaaaacaa
     7081 tggggaatgc tttcaggagg ggaaaaaaag ttcctttatg ctcttatttt gatgtcttta
     7141 gaaagagaat ggtatatttt agatgaaccg tttgcttttg tagataagaa aagaaaaaaa
     7201 atgttatata agattataga tattaagata tcagaagata agggtattat tttaactact
     7261 catgaagaag atgaagtagt attaaaatca gttagcaagg tttttgaaat ataactgtaa
     7321 gaagtagtgt catcatttta gaatatttaa tgttgtacga taaaagaatg aattataata
     7381 agctcagact gtagtttaaa ttatagtaaa tataaaataa ttatgttttt gtatagtagt
     7441 ttttttggaa agtatagata atactggttt ttaatgtttt tgtttgtata gtaaataagt
     7501 tcaaaaagca acgattgtta gctctgctgc tagcagttgt tgctttttga acttagttaa
     7561 atagtgtagt ctatgaaaat taaagtgcta gtgacaaatc tactctacta atttaaataa
     7621 cgtttgtttt ttgtttagat taactcatta aatgtacaat cgtttcaaat taatgagagt
     7681 tttcgtaata aagttttatc tcttttggta agttatttgg aatttttgat ttaacagcca
     7741 cctcgttccc ttgcttaagg acttcatcgt agtaccagca tttaaattta atcatatcaa
     7801 gaactttttc catttgtcgc aattcttttt ctacgcttac tttttgtcgt tcaaatagtt
     7861 cttttcgaat tttgaaagtt tctgacccta attttgtcca tttcataaat tgtttaatat
     7921 ctttaatttc taatccagat ttcttcaaac actcaatcac tcttaaagct tcaatttcgt
     7981 tattagaaaa ttttcgaata ccggaaacac gcgttatttc aggaaataat ccttctttat
     8041 cataatagcg gagagttgaa actggaatgt taaacatttt tgatatttcg ccgattgaat
     8101 atgctgtcat agctcctcct gaaaaatttt tataatcaga cttgacctaa agttaagttt
     8161 aggtagtaag attgtattat taaaaaaatt aaatgtcaac tataggaggg aaagcaagga
     8221 tgctagaaga tactacaatg attcaaaaat gggataaggt attccaaaaa agcgataagg
     8281 ttaatcatcg taatgtgact tttactaatc gatttggaat agctctagtt gctgatttat
     8341 atgaaccaaa aagacaagcg gaagagtttc ctgcaattgc tgtgtgcggt ccgttcgggg
     8401 cagttaaaga acaatcatca ggactttatg ctcaaacaat ggcagaacgg ggctttttga
     8461 cgatagcttt tgatccgtca tttactggag aaagtggagg gatcccacgt aatgttgctt
     8521 ctggcgatat taacactgag gatttccaag cggctgttga tttcttgtcg gtccaaaaaa
     8581 atgttgctca ggataaaatt ggaattattg gtatttgcgg ttggggcggc atggcactaa
     8641 atgcagctgc aattgataca agagtaaaag caaccgttgt ttcgactatg tatgatatgt
     8701 ctcgtgtaac agctaatggt tattttgatt ctattgatgt ggatggtcga tatgaaatgc
     8761 gtcaacaact caatgcacaa cgtattgaag atttcaaaaa tggggattat gctcgttcgg
     8821 gtggtgttcc agatagtttg tcagaaagtg cgccttatta tattaaagat tatttcgatt
     8881 attacaaaac agaccgcggc tatcataagc gatcgttgaa ctcaaatgaa ggttggaata
     8941 tgacgtctgc tctttcattg ttaaatacta aatttctgtc ttatagtgat gagattttta
     9001 gtgcggtgtt aattattcat ggtgaaaaag ctcattctcg atatttcgca gaagacgctt
     9061 ttaagaagtt gcaaggagat aacaaggagt tgctaattat tcctaaagca acacacactg
     9121 atctctacga caaaatagac atcattccat ttgataaaat gaccgaattt ttttatcaaa
     9181 atttggaagt aagataggag gaatttgtaa atgattgaga aacaaactgc tggaagagat
     9241 tttttaggtg aatttgcacc caaatttgct gagctgaatg atgatgtgtt gtttggtcaa
     9301 gtatgggcaa gagaagataa actttctttg aaaatgcgtt ctattgtgac aataacggcg
     9361 ctcatttcaa agggagttat cgattcatct ttgaactatc atctggaaac tgctcggaaa
     9421 aatggtgtaa ctaagacaga aatggctgaa ttattaacac atctttcatt ttatgcagga
     9481 tggccgaatg cttgggcagc gtttcgattg gcgaaagaag tgtatgtgga ggaccataac
     9541 aaagaagaac atgggggatt gtttggtcaa ggggaattaa acacagctta ttcagaatac
     9601 tttattggtc aaagttattt gaaaccattg actgatccaa aagaaacagt gtttattgcc
     9661 aatgtcacgt ttgaaccagc ttgccgtaat aattggcata ttcatcatgc gaaatcagga
     9721 ggaggccaaa tcttgatatg cgtagatggt gaaggttggt atcaagaaga aggaaaagaa
     9781 gcacaaagcc tgaagccagg ggataccatt acaatacctg ccaatgtaaa gcattggcac
     9841 ggtgcaaaga aagatcggtg gtttagccac ttagcgattg aagtgcctgg ggtagaaaca
     9901 tcaaacgaat ggctagagcc tgttaaaaat gaattttatg atagtctaaa ggagtgttaa
     9961 taatgaaagt aagtattgaa gatgttatta atgggtacag aagtgatgct cctgttgatt
    10021 tagagcgaga tgcgtatgct gaggagcttt atcaagaagc gataaaaatt ggaatagaaa
    10081 tgaataccaa gtatcattca gaagaggaat tacgagaaat tatgagttac ttgattggaa
    10141 aaaaagtaga tgaaactttt cgtatatttc caccttttta tacggacttt ggtaaaaaca
    10201 ttacacttgg caaaaatgtc tttataaact cagggacaca tttccaagac caaggtggga
    10261 ttgtgattgg tgatggtgta tttataggac ataatgtagt acttgctaca attaatcacg
    10321 acctatttcc aaagaataaa cgtaaaaatc attatgcgcc aattgtgtta aaaaataacg
    10381 tatggattgg ttcaaatgct acgattactt caggtgtaac gattggtgag tggtcagtag
    10441 ttgcagcagg tgcggtcgtc acaaaagatg ttccacccta cactgtagta ggtggcgttc
    10501 ccgctcgtgt gctaaaatca atagataagg aagagaatgg tagatgattg ataaaacaac
    10561 aaattcaaaa gcagatgaga ctgataatat gaagaaactt tatcttatgc gacatggtga
    10621 aacgctattt aataaattag ggaaaattca aggagcaagt gactcacctt tgactgaagt
    10681 aggaataaaa caagcaagaa ttgcaaaaga gtacttccaa aatcataata ttaaattaga
    10741 tgcttattat tcctctacac aagagcgtgc atcagataca ctagaaataa ttataggaaa
    10801 taaaaaatat aagcgtataa gggaactaaa agaatggaat tttggtatat ttgaaggaga
    10861 aagagaatat ttaaacccta aaagacctat tggggaaact tcatatggtg attcttttgt
    10921 gccttatggc ggggagtctt caaaagtggt gcaggaaaga ataaacaaag gacttactaa
    10981 aattatggaa caagaaaata accagaacgt tttggcagtt agtcatgggg ggctatgtat
    11041 ctat
//
