LOCUS       NZ_KB913032            10813 bp    DNA     linear   CON 28-APR-2024
DEFINITION  Amycolatopsis alba DSM 44262 scaffold1, whole genome shotgun
            sequence.
ACCESSION   NZ_KB913032
VERSION     NZ_KB913032.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Amycolatopsis alba DSM 44262
  ORGANISM  Amycolatopsis alba DSM 44262
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9811274)
  AUTHORS   Klenk,H.-P., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Amycolatopsis alba DSM 44262
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: India
            num_replicons                     :: Missing
            ref_biomaterial                   :: DSM 44262
            biotic_relationship               :: Free living
            rel_to_oxygen                     :: Aerobe
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.05; ALLPATHS v. r41043; Phrap v. 4.24
            finishing_strategy                :: Missing
            environment                       :: Soil
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi10729
            Current Finishing Status          :: Level 2: High-Quality Draft
            Type Strain                       :: Yes
            Funding Program                   :: DOE-CSP 2011
            Isolation Site                    :: Soil
            Cell Shape                        :: Filament-shaped
            Motility                          :: Nonmotile
            Sporulation                       :: Sporulating
            Temperature Range                 :: Mesophile
            Gram Staining                     :: Gram+
            Diseases                          :: None
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.05; ALLPATHS v. r41043; Phrap v.
            4.24
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina GAii
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000384215.1-RS_2024_04_28
            Annotation Date                   :: 04/28/2024 01:36:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,988
            CDSs (total)                      :: 8,922
            Genes (coding)                    :: 8,855
            CDSs (with protein)               :: 8,855
            Genes (RNA)                       :: 66
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 24 of 67
            Pseudo Genes (incomplete)         :: 49 of 67
            Pseudo Genes (internal stop)      :: 4 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:42:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 9151326
            Orig. end                         :: 9162139
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB913032.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 404842
            Source DNA and Organism available from Hans-Peter  Klenk
            (hpk@dsmz.de)
            Source DNA available from Hans-Peter  Klenk (hpk@dsmz.de)
            Organism available from Hans-Peter  Klenk (hpk@dsmz.de)
            Contacts: Hans-Peter  Klenk (hpk@dsmz.de)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10813
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="46"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10813
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10813
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5813](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5813
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            216..1106
                     /locus_tag="AMYAL_RS0142110"
     CDS             216..1106
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157012.1"
                     /locus_tag="AMYAL_RS0142110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_020637334.1"
                     /transl_table=11
                     /translation="MNEPWGIPGPAFAWIYGVCAVLPLALAAANELWLRRDTGARKVPG
                     LYHLAALADGAERVTDTAVAGLLERHSLRPDSRGILHPVRPAPADPFERAVVSLVHGNG
                     IGLRELRSRMARHGVVLELRADLERKDLFVAENRRRTGWLLALALYVAVLALGIARLAN
                     AIPLERAHTILVFLILGVLGALGVTVWRLWPDRSGRATGAGTLALRYGDWDRDLARTTT
                     GQVALNGLRAFPDQVVAITLIKTGGTRARLFAGTASTCGGGASYAVGSSCGGGGSSCGG
                     GSSCGGGGGGGGCGG"
     gene            1103..1660
                     /locus_tag="AMYAL_RS0142115"
     CDS             1103..1660
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157011.1"
                     /locus_tag="AMYAL_RS0142115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_245193345.1"
                     /transl_table=11
                     /translation="MNRLETIYHVAYLAGGPDRVTDTAVAGALERKAARLGSSGVLRRT
                     SAKPADPFVLAAVESLPKVRIARVREVLRESEPMRALRAELLAGGLILPAVHWRRAWHA
                     VTRREVWATKAGERMLFLARKDPSLVTGAAGAVALGSLGAHPDRRLARLLTLTPPSARD
                     RAQVAVGKPPDGLTAVVDCSAG"
     misc_feature    1319..1321
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    1379..1381
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1765..2508
                     /locus_tag="AMYAL_RS0142120"
     CDS             1765..2508
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF036585.4"
                     /locus_tag="AMYAL_RS0142120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_209447285.1"
                     /transl_table=11
                     /translation="MAAVRDLDDAQVDLLCEMADAFGRKADTWEHPDSDLVTDGFGSSM
                     VNRLQLHHATSEEKFNKTSFEFAFKAASRFANRRAEKTVAHTFQGADVVVDGVRWSLKT
                     EGAANMSQDLITISKFSEARWIRDCVTQEDFAREAPARIERHLSEYDRVLTLRGFYVQN
                     HEAVRYDLVEIPHAMLMAATQLTERDFSPRTGAGSSRAQVFYEGKPAFTVSLDGSVEKV
                     TLRNIQVSQCMTHAQWTVPVLPLDD"
     gene            complement(2505..3725)
                     /locus_tag="AMYAL_RS0142125"
     CDS             complement(2505..3725)
                     /EC_number="2.1.1.37"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782794.1"
                     /locus_tag="AMYAL_RS0142125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_020637337.1"
                     /transl_table=11
                     /translation="MERRTFADLFSGGGGMSHGFFARGYEVVGAVDAQVGKPSSGKGTL
                     ECNATFEANMGVAPYDLDLGDVTGAELDDLLEPTLRGRNLDVLSACPPCTGFSRANPNN
                     HLVDDPRNSLIYRVADHVAVLQPKMVVMENARELLQGNFRAHFLEFRHRIESLGYQVYS
                     GVHMLSRFGVPQKRERSLVIAVRGDHELRTLDDLWDGMTLAPEAATVRRAIGHLRPVAA
                     GEADPLDPMHFSPATGTEATRRRLHAMPHDGGSWADLRFREDADELLTPAMQRSVAAGK
                     LGSYPDIYGRMWWDRPAPTIKRECSHFGNGRYTHPEQDRLCTVREMALLTGFPDSYEFR
                     ARATSNMYRHIGDAVPPIVSYQLAAVADWILTGDRPELKDALLSGTHLTAADLVPAKDQ
                     LLLDVTP"
     gene            4061..5008
                     /locus_tag="AMYAL_RS0142130"
     CDS             4061..5008
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157009.1"
                     /locus_tag="AMYAL_RS0142130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_020637338.1"
                     /transl_table=11
                     /translation="MKDTWGIPGPAFLAIYAVLLVVPLVVGLLWTIAVKLGRKAPRAAV
                     SGPQPTVYELAYLAGGPDRVVDTAIAALVERGILRVSSSKQLQLAGAVPADPIERAVAK
                     GARPGYNATTRGIRDRLRMSGPMQALAKDLEVRGLVVVNQAPRIRQVVFFLYVAVFVLG
                     VVRLFAGLATSHAVGFLVPLLIAAGIATLIARSVKNKTTGPRPTSEGNRILGSANSAVA
                     RERKRGIPNQPAVGGVGPGFLLGGAAAAVALGGLAFYPDEELSAALIPPPMPSAFGGGG
                     SSGGSTCSTGSSCSSGSSSSCGGGSSCGGGGCGG"
     gene            5001..5813
                     /locus_tag="AMYAL_RS0142135"
     CDS             5001..5813
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157007.1"
                     /locus_tag="AMYAL_RS0142135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_026467902.1"
                     /sec_met_domain="DUF692 (E-value: 4.6e-82, bitscore: 274.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADGPGRLGIGIGWRHELDLSIARLPGVDWVEVVAENLHTGHLPE
                     TLIALRDKGMPVLPHAVSLSLGGAEPLDTTRVEHLAEVTRELGAPMASDHVCFVRAGGL
                     DSGHLMPLPRTREALDVLVDNVKLTQSIVEVPFALENIAAVLEWPDAEFTESEFLRELT
                     DRTGCLLIIDVANLYANARNLGTDPERFLDEIPWERLAYAHMAGGVERDGVYHDTHAHP
                     VVPEVLDLLRELRRRTEPPGVLLERDDDYPPDAELAAELAALREAVSP"
     gene            5810..6178
                     /locus_tag="AMYAL_RS50245"
     CDS             5810..6178
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157004.1"
                     /locus_tag="AMYAL_RS50245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_020637340.1"
                     /transl_table=11
                     /translation="MTSPRERLAGQQAELLKALLAGGDAPAGFDADRLRIEANVLRNKQ
                     SRLAAYLRPDLAEALGDRFAALFREYSTTHPKTDSIRARAYADAFGTWLVERGEVPKPK
                     RGLSTWAPWRGGLRRRRS"
     gene            complement(6129..6452)
                     /locus_tag="AMYAL_RS0142145"
     CDS             complement(6129..6452)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157001.1"
                     /locus_tag="AMYAL_RS0142145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_245193346.1"
                     /transl_table=11
                     /translation="MRRKTAKLTLIAASALVLCACGVDGKIGDYGDTTVYSEPKPNSNG
                     GVSNDPVGTLTTLSKVTVSCYTKVNGFGFYKISYSGGSGYIDDSTSIMSDDGEVRPAKV
                     PKC"
     misc_feature    6481..6498
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 20.11"
                     /tool="antismash"
     gene            complement(6496..7842)
                     /locus_tag="AMYAL_RS0142150"
     CDS             complement(6496..7842)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1189: L-carnitine dehydratase/bile acid-inducible"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033530.1"
                     /locus_tag="AMYAL_RS0142150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CoA transferase"
                     /protein_id="WP_039796110.1"
                     /transl_table=11
                     /translation="MDTRVWTTLTGDKLAEDAVEVTGPESVLPGTFRVEEAATTSIAVA
                     TLAAGELLRLRGIEPGKVSVDTRHAAASFHSEQYLRVDGEPVPFSAPLSGDYRANDGWV
                     RLHCNYPRHVSAVCWGLGVPATRAAFEKVVAAKSKFEVESAVVGAGGAAAVLRSREEWL
                     AHKQGQASSALPVAEIRSLGTSEPVRLFDSDRPLGGVRILDLTHVIAGPVAGRVLAAHG
                     ATVMHIGSAQLPTVPPLAVDTGMGKLSAYVDLETEAGRSRLRKLIARADVVLQGFRPGS
                     LVAKGFSPEAIAGLRPGIVVADLSAYGWEGPWARRRGFDSLVQMASGIADEGGKTAGLD
                     GPGPLPVQALDHATGWLTAAAIMTAVRQAATEGGSRHVRTSLAGTGEWLNSLGRKEAGP
                     AGFDGAEFLEDADSPLGRLTRVKMAGTLPGSNPFWVEGTRVPGSDRAVW"
     gene            complement(7854..8546)
                     /gene="hemQ"
                     /locus_tag="AMYAL_RS0142155"
     CDS             complement(7854..8546)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /locus_tag="AMYAL_RS0142155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_020637343.1"
                     /transl_table=11
                     /translation="MARLNYNELNDTIRYTAWSVFRAEQGKLGEDRAKATAETTDYLDS
                     LEAKGVVVRGLYDLSGLRADADYMVWWHAEEIEQVQAAYSGFRHTPLGRASAPVWSQVA
                     LHRPAEFNKSHIPAFLAGEEARKFICVYPFVRSYEWYLLPDDERRKMLADHGKEARDYP
                     DVRANTVASFALGDYEWILAFEADELHRIVDLMRHLRGTEARLHVREEIPFYTGTKVEP
                     AELIALLP"
     gene            complement(8572..9951)
                     /gene="hemG"
                     /locus_tag="AMYAL_RS0142160"
     CDS             complement(8572..9951)
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332996.1"
                     /locus_tag="AMYAL_RS0142160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_020637344.1"
                     /transl_table=11
                     /translation="MKTVAVVGGGISGLTAAYRLRTLLGEDARIVVFEATESLGGKLRT
                     IELAGERYDVGAEAFLARRPEALALVREAGLGESLVHPTKARAKIHAGGSVLGLPPGTV
                     MGVPASAGAVAGVLSEKGRALVEAESSLPPVELPAGDVPLGPLLRERFGDELVDRLVDP
                     LLGGVYAGGADGLGLRATMPGLASAVDRGARSLTEGAASLMPASPSTAPVFGTLLGGLG
                     TLIARLAELSRAEIRTGTTVTALERTATGWKVDDLEADSVLLAVPAPSARRLLDGVAGV
                     ASSAFAEVELASMAVVALALPPGTALPESSGVLIGAGERDEAGEPFAAKAFTFSSRKWA
                     QYGDGPVLVRGSVGRFGEPGALHADDDELVRVVRDDLARLSGITAEPIETLVTRWGGGL
                     PQYGTGHLERVERIERAVAEVPGLAVAGAALHGVGLPACIATADAAARRIAGITPTHEG
                     S"
     misc_feature    10738..10753
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.86"
                     /tool="antismash"
ORIGIN
        1 gccggatcga ggtgctgtgc aagtcgtgcc acagccacct cgggcacgtc ttcgaaggcg
       61 agggctatgc cacgccgacg gatcagcggt actgcatcaa ctcgatctcg ctgaagctcg
      121 ttcccgagtc cgagtcgtaa ccgaaccgag cgaaccaccg cccccctcca cgcgttctcc
      181 tagaactgga acagcgtcgg ggagggcggt gcttcatgaa cgaaccctgg ggaatccccg
      241 gaccggcttt cgcctggatc tacggtgtct gcgccgtatt gccgctggca ctcgccgcgg
      301 cgaacgagct gtggctgcgc cgcgacaccg gtgccaggaa ggtgcccggt ctctaccacc
      361 tggccgcgct ggcggacggc gcggaacgcg tcaccgacac cgccgtcgcc ggtctgctcg
      421 aacggcactc actccggccc gacagccggg ggatcctgca cccggtcagg cctgctccgg
      481 ccgatccctt cgagcgcgcc gtcgtctcgc tcgtgcacgg caacgggatc ggcctgcgtg
      541 aactgcgctc ccggatggcg cggcacggcg tcgtcctgga gctgcgcgcc gacctggagc
      601 ggaaggacct gttcgtcgcc gaaaaccggc gacggacggg ctggctgctc gctcttgccc
      661 tctacgtggc cgtcctcgcg ctgggcatcg cgcggctggc gaacgccata ccgctggagc
      721 gggcgcacac catcctcgtc ttcctgatcc tcggcgtcct cggcgcactc ggcgtgaccg
      781 tgtggcggct atggccggac aggtccggcc gcgcgaccgg cgcgggcacg ctcgcgctcc
      841 ggtacggcga ttgggaccga gacctggcac gcaccacgac cgggcaggtc gccctcaacg
      901 ggctgcgggc cttccccgac caggtcgtcg cgatcacgct gatcaagacc ggcggcacga
      961 gggccaggtt gttcgcgggc acggcgagta cctgcggcgg aggcgcgtcc tacgccgtcg
     1021 gctcctcgtg cggcggtgga gggtcctcct gtggcggcgg ctcttcctgc ggcggcggag
     1081 gaggaggagg agggtgcggc ggatgaaccg gctcgaaacg atctaccacg tcgcttacct
     1141 cgccggtggc cccgaccggg tcacggacac cgccgtcgcg ggagccctcg aacggaaagc
     1201 ggcccggctc ggcagttccg gtgtgctgcg ccggacctcc gcgaagcccg ccgatccgtt
     1261 cgtgctggcg gctgtggagt ccttgcccaa ggtacgcata gcgcgggtgc gcgaagtctt
     1321 acgcgagtcg gagccgatgc gcgcgctgcg cgcggaactc ctggccggcg gcctgatctt
     1381 accggcggtc cactggcgac gggcttggca cgccgtcacg cggcgcgagg tgtgggcgac
     1441 caaggcgggc gaacggatgc ttttcctggc gagaaaggat ccgagtctcg tcaccggtgc
     1501 ggcgggcgcg gtggcgctgg gcagtctcgg cgctcatccg gatcggcggc tggcgcggct
     1561 cctgaccctg acgccccctt ccgcccgtga cagagcccag gtcgccgtcg gcaaaccccc
     1621 tgacggtcta acggccgtcg tggactgttc ggcaggctag gcggtgaact tgcctatctt
     1681 gctgcggccg ctgagtctcc tcagtagtct cgacagcatg ctgatcgccg attccccttc
     1741 tatcgcatga gcgttgagtg gctcatggct gccgtgcggg atcttgacga cgcgcaggta
     1801 gacctcctct gcgagatggc ggacgccttc ggccggaaag ccgacacgtg ggagcatccc
     1861 gacagcgatc tcgtgaccga cggcttcggc agttccatgg tgaaccgcct gcagcttcac
     1921 cacgcgacgt cagaggagaa gttcaacaag acctctttcg agttcgcgtt caaagcagcg
     1981 tcgaggttcg cgaaccggcg agccgagaag accgttgccc acaccttcca aggcgccgac
     2041 gtggtggtcg acggcgtgag gtggtccctc aagacagagg gagccgcgaa catgagccag
     2101 gatctcatca ccatctccaa gttctccgaa gcgcgctgga ttcgggactg tgtcacacaa
     2161 gaggacttcg cacgcgaagc tccagctcgc atagaacgcc acttgagcga gtacgaccgt
     2221 gtgctcacgc ttcgaggctt ctacgtccag aatcacgaag cggtccgcta cgacctcgtc
     2281 gagatcccgc acgcgatgct catggctgcc acgcagctca ccgaaagaga tttctcacct
     2341 cgtaccggag ctggttcgag ccgcgcgcag gtgttctacg aaggcaagcc cgcctttacc
     2401 gtcagcctgg atggaagcgt cgagaaggtc acgctgcgca atatccaagt gagccagtgc
     2461 atgacccatg cccaatggac cgtgcctgtc ctgcccctcg acgactaggg cgtcacgtcg
     2521 agcagcagct gatcctttgc gggaacgagg tcggctgctg tcaggtgagt tccggacagc
     2581 agggcatcct tgagttcggg acggtctccg gtgaggatcc agtccgcgac ggcggcaagc
     2641 tggtaggaca cgatcggcgg caccgcgtca ccgatgtggc ggtacatgtt cgacgttgct
     2701 cgcgccctga actcgtagga gtcggggaag ccggtcagca gtgccatttc ccgcacggtg
     2761 cagagcctgt cctgctcggg gtgggtatag cgcccgttgc cgaaatgcga gcactcgcgc
     2821 ttgatcgtcg gggccgggcg atcccaccac atccgcccgt agatgtcggg ataagagccc
     2881 aattttccag ccgccacact gcgctgcatc gcgggcgtca aaagctcgtc cgcgtcctcc
     2941 cggaaacgca ggtccgccca ggagccgccg tcgtggggca tcgcgtgaag gcgccgtcgg
     3001 gtggcttccg tccccgtggc cgggctgaag tgcatcgggt ccaagggatc ggcttcgcct
     3061 gccgcaacag ggcgaagatg cccgatggcc cggcgtacgg tcgcagcctc gggagcgaga
     3121 gtcatcccgt cccagagatc gtcgagggta cgaagctcgt ggtcgccgcg gaccgcgatg
     3181 acgagtgagc gctcgcgctt ctgaggcacc ccgaaccggg acagcatgtg cacgcccgag
     3241 tagacctggt agcccaagct ctcgatccgg tgacggaact ccaggaagtg cgccctgaag
     3301 ttgccctgga ggagctccct cgcgttctcc atcacgacca tcttcggctg caggacggcg
     3361 acgtggtcgg ctacgcgata gatgagcgag ttacgagggt catccaccag atggttgttc
     3421 gggttggccc gtgagaagcc ggtacaagga gggcatgcgc tgaggacatc caggttccgt
     3481 ccccgcagag tgggttcgag caggtcatcg agctcggcac cggtcacgtc gccgaggtcg
     3541 aggtcatagg gggcgacacc catgttggcc tcgaaagtcg cgttgcactc gagtgtcccc
     3601 ttgccgctgc tgggcttgcc cacctgggcg tcgacagcac cgacgacttc gtagccacga
     3661 gcgaagaacc catggctcat cccgcccccg ccactgaaca agtctgcgaa ggtgcgtcgt
     3721 tccactgctt ctcctcgtgc cgttccgaga ccgcggcggc ggggaaggca gcgtcggtct
     3781 cggccgagta taggtgctgt ccggctcgcc cctccggcac cgtgacggcg cgtcagcgca
     3841 tgctgtggac ggctcgccga ggctcgttcc cgagtcgtga ccaagccggg cgaaccacct
     3901 ccgtccccgg cgcgttcaca caggacaacg cagtgccggg cggctgacgg cccaactgcc
     3961 gtcgtgcgag tccacgccgg ctgttcgaat cagggaacca cgaccgggcg gaaaagcgtc
     4021 cgactacggt aagtgcacct gctcgtgaaa ggcccgtgcg atgaaagaca cctggggcat
     4081 ccccggaccg gctttcctgg cgatctacgc agtcctgctg gtggttccgc tggtcgtcgg
     4141 tctcctgtgg acgatcgcgg tcaagctggg acgcaaggcg ccgcgcgccg ccgtgagcgg
     4201 gccgcagccg acggtgtacg agctcgcgta cctggcgggc ggcccggacc gggtggtcga
     4261 cacggccatc gcggcgctgg tcgagcgcgg gatcttgcgg gtcagcagct cgaagcagct
     4321 tcagctggcc ggagccgtgc cagccgaccc gatcgaaagg gccgtcgcca agggcgccag
     4381 gccgggctac aacgccacga cccgcgggat tcgcgaccgg ctgcggatgt ccgggccgat
     4441 gcaggcgctc gccaaggatc tggaggtgcg ggggcttgtg gtcgtcaacc aggcgccgcg
     4501 tatccgccag gtcgttttct tcctgtacgt cgccgtgttc gtcctcggcg tggtgcggct
     4561 gttcgccggg ctcgccacga gccacgcggt cggcttcctg gtccccctgc tgatcgcggc
     4621 cgggatcgcg acgctgatcg cccggtccgt caagaacaag acgaccgggc cgcgcccgac
     4681 cagtgagggc aaccggatcc tcgggagcgc caacagcgcg gtggcgcggg aacgcaagcg
     4741 cgggatcccc aaccagcctg ccgtgggcgg cgtgggtccc ggcttcctgc tgggcggcgc
     4801 ggccgcggcc gtcgcgctcg gcgggctcgc cttctacccg gacgaagaac tgagcgcggc
     4861 gctgatcccg cctccgatgc ccagcgcttt cggcggcggt ggttcgtccg gtggcagcac
     4921 ctgcagcacc ggctcctcgt gcagcagcgg aagctcctcc tcgtgcggcg gcggcagttc
     4981 ctgcggtggc ggcggctgcg gtggctgacg gacccggccg cctcgggatc gggatcggct
     5041 ggcggcacga gctggacctg tcgatcgcgc ggctgcccgg cgtcgactgg gtcgaggtcg
     5101 tcgccgagaa cctgcacacc gggcatcttc cggagacgtt gatcgccttg cgcgacaagg
     5161 gaatgccggt cctgccgcac gcggtctcgc tgtcgctggg cggggccgag ccgctggaca
     5221 ccacgcgcgt cgagcatctc gcggaggtca cccgtgagct cggcgcgccg atggcgagcg
     5281 atcacgtgtg cttcgtgcgc gcgggcggcc tcgactcggg gcacctgatg ccgctccccc
     5341 gtacacggga agcgctcgac gtcctggtgg acaacgtcaa actgacccag tccatcgtgg
     5401 aggtcccgtt cgcgctggag aacatcgcgg cagtgctgga atggccggac gccgagttca
     5461 ccgagtcgga attcctgcgg gagctgaccg accggaccgg ctgcctgctg atcatcgacg
     5521 tcgccaacct gtacgccaac gcccgcaacc tcggcaccga tcccgagcgg ttcctcgacg
     5581 agatcccgtg ggagcggctg gcgtacgcgc atatggccgg cggcgtcgaa cgcgacggcg
     5641 tctaccacga cacgcacgcc catccggtgg tcccggaagt gctggacctg ctgcgcgaac
     5701 tgcggcggcg taccgagccg ccgggtgtcc tgctggaacg cgacgacgac tacccgcccg
     5761 acgcggaact cgcggccgaa ctggccgcgc tccgggaagc ggtctcgccg tgacttcgcc
     5821 ccgcgagcgg ctcgccgggc agcaggcgga gctgctcaag gcgctcctcg ccggcggtga
     5881 cgccccggcg gggttcgacg cggacaggct ccgcatcgag gcgaacgtgt tgcggaacaa
     5941 gcaaagcagg ctggccgcgt acctgcggcc ggacctcgcc gaggccctcg gcgaccggtt
     6001 cgccgcactg ttccgcgagt actccacaac gcacccgaag accgactcca tccgcgcgcg
     6061 ggcctacgcg gacgctttcg gcacgtggct cgtcgaacgc ggcgaggtcc cgaaaccgaa
     6121 gcgcgggctc agcacttggg caccttggcg gggcggactt cgccgtcgtc gctcatgatc
     6181 gaggtcgagt cgtcgatgta gcccgagccg cccgagtacg agatcttgta gaacccgaac
     6241 ccgttgacct tcgtgtagca ggaaacggtc accttggaca aggtggtcag ggtgcccacc
     6301 gggtcgttgc tcaccccgcc gttcgagttg ggtttcggct ccgagtagac cgtcgtgtcg
     6361 ccgtagtcgc cgatcttccc gtcgaccccg cacgcgcaca gaaccagcgc ggacgccgcg
     6421 atcaaagtca gcttcgcagt ctttcgtctc ataccgacga agttaggtcg ggaagatgaa
     6481 aatccgatga agcggctacc agaccgcgcg gtcgcttccg gggacgcgcg tcccttccac
     6541 ccagaaggga ttcgagccgg gcagcgtgcc cgccatcttg acgcgggtga gccgtccgag
     6601 cggactgtcg gcgtcctcca ggaactcggc gccgtcgaag ccggctggcc cggcttcctt
     6661 gcgccccaag gagttcagcc actcccccgt tccggcgagc gacgtgcgga cgtgccggct
     6721 gccgccttcg gtggccgcct ggcggacggc ggtcatgatc gccgccgcgg tgagccagcc
     6781 ggtcgcgtgg tcgagcgcct gcaccggaag cggtcccgga ccgtcgagcc cggcggtctt
     6841 gccgccttcg tcggcgatcc ccgacgccat ctgcacgaga ctgtcgaacc cgcgcctgcg
     6901 cgcccacggc ccttcccagc cgtaggccga caggtccgcg accacgatcc ccggccggag
     6961 cccggcgatc gcctccggcg agaaaccctt ggccaccagc gatcccggcc ggaaaccctg
     7021 gagcacgaca tcggcacgcg cgatcagctt gcgcagccgc gagcgcccgg cctcggtctc
     7081 cagatcgacg tacgccgaga gcttgcccat cccggtgtcg acggccagcg gcggcacggt
     7141 cggcagctgc gccgacccga tgtgcatgac ggtggcgccg tgcgcggcga gcacgcggcc
     7201 cgcgaccggc ccggcgatga cgtgggtcag atccaggatc cgcaccccgc ccagcggccg
     7261 gtcggagtcg aacagccgca ccggttccga cgtcccgagc gaccggatct cggcgaccgg
     7321 cagcgcgctg gaggcctgtc cttgcttgtg cgccagccat tcctcgcggc tgcgcaacac
     7381 cgcggcggca ccaccggcgc cgaccaccgc cgactcgacc tcgaacttcg acttggccgc
     7441 cacgaccttc tcgaacgccg cacgggtcgc gggcacgccg agaccccagc acacggcgga
     7501 gacatggcgc gggtagttgc agtgcagccg cacccagccg tcgttggcgc ggtagtcgcc
     7561 ggacagcggc gcgctgaacg gcaccggctc cccgtcgacg cgcaggtact gctcactgtg
     7621 gaacgacgcc gcggcgtgcc gggtgtcgac cgagaccttg ccgggctcga ttccccgcag
     7681 ccgcaacagt tccccggcgg ccagcgtcgc gacggcgata ctcgtcgtcg ccgcctcctc
     7741 gacccggaag gtccctggca gcacggactc ggggccggtg acctcgacgg cgtcctcggc
     7801 gagcttgtca ccggtgagcg tggtccagac gcgggtgtcc actgaggcct tcactagggc
     7861 aggagggcga tcaactcggc gggttccacc ttggtcccgg tgtagaacgg gatctcctcg
     7921 cgcacgtgga ggcgggcctc ggtgccgcgc aggtgccgca tcaggtcgac gatgcggtgc
     7981 agttcgtcgg cctcgaaggc caggatccac tcgtagtcgc ccagcgcgaa cgacgcgacc
     8041 gtgttggcgc ggacgtccgg gtagtcgcgg gcctccttgc cgtggtcggc gagcatcttg
     8101 cggcgctcgt cgtccggcag caggtaccac tcgtaagagc gcacgaacgg atagacgcaa
     8161 atgaacttgc gggcctcttc accggcgagg aacgccggga tgtggctctt gttgaactcg
     8221 gccgggcggt gcagcgcgac ctggctccag accggcgccg aggcgcggcc gagcggggtg
     8281 tgccggaaac cgctgtaggc cgcctggacc tgctcgatct cctcggcgtg ccaccagacc
     8341 atgtaatcgg cgtcggcccg cagccccgac aggtcgtaga gaccgcggac gacgacgccc
     8401 ttggcctcca gggagtcgag gtagtcggtg gtctccgcgg tcgccttcgc gcggtcctca
     8461 ccgagcttgc cctgctcggc ccggaagacc gaccaggcgg tgtagcggat ggtgtcgttg
     8521 agctcgttgt agttcagccg cgccataccg ccatcttggc actgcccgcg ctcaggagcc
     8581 ctcgtgggtc ggtgtgatcc cggcgatgcg gcgggcggcc gcgtccgcgg tcgcgatgca
     8641 ggccgggagc ccgacgccgt gcagcgccgc gcccgccacg gcgagaccgg ggacctccgc
     8701 gacggcgcgt tcgatccgct cgacacgttc gaggtgaccg gtgccgtact gcgggagccc
     8761 gccgccccac cgggtcacca gtgtctcgat cggctccgcg gtgatcccgc tcagccgggc
     8821 gagatcgtcg cggaccacgc gcacgagttc gtcgtcgtcc gcgtgcagcg cgcccggttc
     8881 gccgaagcgg ccgaccgagc cgcggaccag caccgggccg tcgccgtact gcgcccactt
     8941 gcgggaggag aaggtgaacg ccttggcggc gaacggctcg cccgcctcgt cgcgttcacc
     9001 cgcgccgatc agcacgccgg acgactccgg cagtgcggta cccggcggga gcgcgagcgc
     9061 gaccaccgcc atcgacgcga gttcgacctc ggcgaacgcc gacgacgcga cacccgcgac
     9121 gccgtcgagc agccgtcgtg ccgaaggcgc cgggacggcg agaaggaccg agtcggcttc
     9181 gaggtcgtcg accttccagc cggtggcggt gcgctcgagc gccgtcaccg tggtcccggt
     9241 ccggatctcg gcgcgcgaca gctccgcgag ccgcgcgatc agcgtgccga ggccgcccag
     9301 cagggtgccg aaaaccggag ctgtgctcgg ggacgcgggc atgagcgaag ctgcgccttc
     9361 ggtgagcgaa cgcgcgccgc ggtccaccgc ggaggcgaga ccgggcatcg tcgcgcgcag
     9421 cccgaggccg tccgctcccc cggcatagac cccgcccagc agcgggtcga cgagccggtc
     9481 cacgagttcg tcgccgaagc gctcgcgcag cagcgggccg aggggcacgt caccggcggg
     9541 cagctccacc ggcggcagcg aggactcggc ctcgaccagg gcgcggccct tttccgacag
     9601 caccccggcg accgcgcctg ccgaggcggg aacgcccatg accgtgcccg gcggcaggcc
     9661 gagcacggat ccgccggcgt ggatcttcgc gcgggccttg gtggggtgga cgaggctttc
     9721 gcccagcccg gcctcgcgca ccagggcgag ggcttcgggg cggcgggcga ggaacgcctc
     9781 ggcgccgacg tcgtagcgtt caccggcgag ttcgatcgtg cggagtttcc cgccgaggga
     9841 ctccgtcgcc tcgaagacga cgatccgggc gtcttcaccg agcagcgtcc gcagccggta
     9901 cgccgcggtc agtccggaga taccgccacc gacgacggcg accgtcttca ctgctggatc
     9961 tcgtgcacca gcccgaccac gcgcgtcagg acgtccgggt cgacgccggg cagcaccccg
    10021 tggccgaggt tgaagatgtg gccgtcggcc gcgcggcctt cggcgaggat ccgccggacc
    10081 tccgcttcga gcaccggcca ggacgcgtgc agcagcgccg ggtcgaggtt gccctgcacg
    10141 accccgccgc cgagccgccg gacggcctcg tcgagcggga tgcgccagtc gacgccgacc
    10201 acgtcggcgc ccgcgtcgcg catggccacc agcagctcgc cggtgccgac gccgaagtgg
    10261 atccgcggca cgccgtagcc cgcgaccgcg tccagcacct tcgccgaatg cggcaggacg
    10321 aactcgcggt agtcccgctc cgacagcgcg cccgcccacg agtcgaacag ctggatcgcg
    10381 tcggctccgg cgtcgagctg cgcggacagg aaggtgatcg ccatgtccgc cagtcgcccg
    10441 gccagctcgt gccacagcgc cggttcggag tgcatgagcg ccttggtgtg ctcgtggttg
    10501 cggctcggcc cgccctcgat gagataggag gccagggtga acggcgcgcc cgcgaaaccg
    10561 atcagcggtg tctcgcccag cgacccgacc agcagccgga cgccgtcggc gacccgttcc
    10621 acctgctccg ggtccagcac cggcagcgcg cgcacgcccg cggcgtcgcg gatcggctcg
    10681 gcgacgaccg ggccggtgcc cgcgacgatg tcgatgtcga ggcccgccgc cttgagcggc
    10741 acgacgatgt cactgaagag gatggccgcg tcgacgccgt gacggcggat cggctggagg
    10801 gtgatctcgg cga
//
