LOCUS       NZ_KB913032            20291 bp    DNA     linear   CON 28-APR-2024
DEFINITION  Amycolatopsis alba DSM 44262 scaffold1, whole genome shotgun
            sequence.
ACCESSION   NZ_KB913032
VERSION     NZ_KB913032.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Amycolatopsis alba DSM 44262
  ORGANISM  Amycolatopsis alba DSM 44262
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9811274)
  AUTHORS   Klenk,H.-P., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Amycolatopsis alba DSM 44262
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: India
            num_replicons                     :: Missing
            ref_biomaterial                   :: DSM 44262
            biotic_relationship               :: Free living
            rel_to_oxygen                     :: Aerobe
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.05; ALLPATHS v. r41043; Phrap v. 4.24
            finishing_strategy                :: Missing
            environment                       :: Soil
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi10729
            Current Finishing Status          :: Level 2: High-Quality Draft
            Type Strain                       :: Yes
            Funding Program                   :: DOE-CSP 2011
            Isolation Site                    :: Soil
            Cell Shape                        :: Filament-shaped
            Motility                          :: Nonmotile
            Sporulation                       :: Sporulating
            Temperature Range                 :: Mesophile
            Gram Staining                     :: Gram+
            Diseases                          :: None
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.05; ALLPATHS v. r41043; Phrap v.
            4.24
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina GAii
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000384215.1-RS_2024_04_28
            Annotation Date                   :: 04/28/2024 01:36:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,988
            CDSs (total)                      :: 8,922
            Genes (coding)                    :: 8,855
            CDSs (with protein)               :: 8,855
            Genes (RNA)                       :: 66
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 24 of 67
            Pseudo Genes (incomplete)         :: 49 of 67
            Pseudo Genes (internal stop)      :: 4 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:42:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8244093
            Orig. end                         :: 8264384
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB913032.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 404842
            Source DNA and Organism available from Hans-Peter  Klenk
            (hpk@dsmz.de)
            Source DNA available from Hans-Peter  Klenk (hpk@dsmz.de)
            Organism available from Hans-Peter  Klenk (hpk@dsmz.de)
            Contacts: Hans-Peter  Klenk (hpk@dsmz.de)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20291
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RRE-containing"
                     /region_number="40"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /tool="antismash"
     cand_cluster    1..20291
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /kind="single"
                     /product="RRE-containing"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20291
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10291](+)"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10291
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
     misc_feature    631..644
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.8"
                     /tool="antismash"
     gene            complement(1540..3168)
                     /locus_tag="AMYAL_RS0138310"
     CDS             complement(1540..3168)
                     /GO_function="GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166162.1"
                     /locus_tag="AMYAL_RS0138310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="WP_020636598.1"
                     /transl_table=11
                     /translation="MANRAARGDEADYVVVGSGSSGAAIAGRLAESGASVIVLEAGKSD
                     ERMLVRKPGLVGPMHAVPQLKKPFDWGYYSVPQKHVLDRRMPVPRGKVVGGSSSINGMV
                     YVRGNRANFDSWAAEGNKGWDADSVNAAYKRMEDFEDGESEFRGKGGPIRVTRNKIPQE
                     GTLQFVQATADAIGCEILDDYNAESQEGVSRMQQNAADGLRYSASRGYIHELAPATLQV
                     QSGVLAKKVLVENGRAVGVEVVDADGSRRTLRAGKEVILSAGFVGSAQLLMLSGIGHAE
                     HLKEHGIDVVADLPVGDNLHDHMFHALTFRASSSKNKGTPPYFATGLVKELMRPGTTFL
                     ANSVFEAVAFLKTSQATTIPDLQLHLLPWAYVTPNQDAPIRHDVDKRPALTVLTTLIYP
                     KSRGTLRLASADPTAAPLIDFQYLSDPADLEVLGEGSEMVREIFSSKAFNGSIKEELHP
                     GKALQGQELRDAILNRATSVYHGVGTCRMGVDELAVVGPDLKVRGVEGLRVCDASIMPS
                     ITGGNTNAPAIMIGEMGAQLVLSDN"
     gene            3488..3625
                     /locus_tag="AMYAL_RS50040"
     CDS             3488..3625
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166163.1"
                     /locus_tag="AMYAL_RS50040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_020636599.1"
                     /transl_table=11
                     /translation="MRGVVLVASAGTAVAYVVLTVEIWALLFGFSTCAALVLACLARRR
                     "
     gene            complement(3690..4529)
                     /locus_tag="AMYAL_RS0138320"
     CDS             complement(3690..4529)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034596.1"
                     /locus_tag="AMYAL_RS0138320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="WP_020636600.1"
                     /transl_table=11
                     /translation="MRFAIKTSPQDTVWSDMLAVWQAADEIELFESGWTFDHFYPIFSD
                     STGPCLEGWVTLTALAQATKRLRLGTLVSGIHYRHPALLANMAATLDIVSGGRLEIGIG
                     AGWNEEESGAYGMELGTIKERSDRFEEGCEVLVGLLTQETTTFQGEHYQLTDARCEPKG
                     VQKPHPPICIGGSGEKRTLRTAAKYAQHWNFVGGTPEEFAHKREVLHRHCADIGRDPSE
                     ITLSSHVRLSADGDYAKVAADAEALGEAGLDLAIIYLPPPHTPAVLEPLAKALEPLR"
     misc_feature    4641..4655
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.58"
                     /tool="antismash"
     gene            4703..5047
                     /locus_tag="AMYAL_RS0138325"
     CDS             4703..5047
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333707.1"
                     /locus_tag="AMYAL_RS0138325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_020636601.1"
                     /transl_table=11
                     /translation="MRARSAFLAVTGVVALFLTIPATASAAEGEFTYRYYDEHEEIQVG
                     VLVDPPSGECIEIPEIADWDVDAFRPRNDTDSTAVVFMDDDCSSDAYFSLRPNGGRGSD
                     RLLLRSVIFS"
     gene            complement(5040..5696)
                     /locus_tag="AMYAL_RS0138330"
     CDS             complement(5040..5696)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034600.1"
                     /locus_tag="AMYAL_RS0138330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="S1 family peptidase"
                     /protein_id="WP_020636602.1"
                     /transl_table=11
                     /translation="MRLPRSRFLLGLVLVLWVAWPPTAQADAPAPLGGGSVLQSRCTAG
                     FAAVAGNTGYLIASSACGRPGDTVRSAGRVVGVVVADQVPPTGAIVIRVTNTTDWRLVG
                     SIPPVDSRVTITGSAEAPVGASVCRYGSTTGWHCGTIQGKNQTITFPGGTLTGLTRTSV
                     CAEPGDAGGPFVSGTQAQGVLVGGSGNCSTGGTTYFLPVNRVLSAYGLTILAGTS"
     gene            complement(5739..6761)
                     /locus_tag="AMYAL_RS0138335"
     CDS             complement(5739..6761)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1251: luciferase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166167.1"
                     /locus_tag="AMYAL_RS0138335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_020636603.1"
                     /transl_table=11
                     /translation="MKKIGFLSFGHWSASAHSETRSAADFLLQSIDLAVAAEELGVDGA
                     YFRVHHFARQAGSPFPLLSAIGARTSKIEIGTGVIDMRYENPMYMAEDAGAADLISGGR
                     LQLGVSRGSPEQVIDGWRYFGYGPAEGETAADMARQRTEVFLKVLDGEGFAQPNPRPMF
                     ANPPGLLRLEPYSEGLRDRIWWGSSSNATAVWAAELGMNLQSSTLKDDETGEPLHVQQR
                     KQIEAYREAWKEAGHEREPRVSVSRSIFALTSDLDRAYFGRDRDSRDQVGMIDETTRAI
                     FGRSYAAEPDELVRELKADEAIQAADTLLLTIPNQLGVEYNAHVLESILTHVAPELGWR
                     "
     tRNA            7024..7107
                     /anticodon=(pos:8251150..8251152,aa:Leu,seq:gag)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="AMYAL_RS0138340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /product="tRNA-Leu"
     gene            7024..7107
                     /locus_tag="AMYAL_RS0138340"
     gene            complement(7499..8608)
                     /locus_tag="AMYAL_RS0138345"
     CDS             complement(7499..8608)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AMYAL_RS0138345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_020636604.1"
                     /transl_table=11
                     /translation="MLRDNLVGTVLKLDMTGACNWPDGEQPVLLTKFVGEGAESVVYSI
                     APLSEPTQDDVVLKLPKTAPYFEMDTLHHSFAVHTELYPEHPLAMSPPDRLEMLKSEML
                     AKVADPHLVFRIDSYREIIDASISILTMAFADGPVPLDDSPVREWIDDNLVHRATELLD
                     EDLIVEQHRENLECALAEAEAAIVRWRASESYVPVSANPLVMLAGLLFEGFISEQEMSW
                     LARTQELGDRLVPEHLPGVVAAVATMYHRRAGEKVSDRVRRPKRHAPDLVAACDLFAAA
                     GTHFPDHANWCEAMADGWRGRTLLLTGHPLAEVTASLENARAIWLRLGELAEYHDTLRD
                     LAEAHLRNDPDSAAEYLAELRAVRQALGR"
     misc_feature    complement(7694..7696)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(8610..9506)
                     /locus_tag="AMYAL_RS0138350"
     CDS             complement(8610..9506)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AMYAL_RS0138350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_020636605.1"
                     /transl_table=11
                     /translation="MTELVNVASGISGFTVQARDIEGGVHFHFTVPDRDGVVRCGELSE
                     AAAAALEIALHDLEARLDSLGVADPRENAEEITRSVLLAFREDRNSHLPVLSEPRLATL
                     VGDITAFAEPARLLAQRFYFSTRKVATLHRAYRHLLPDIEEPEQRFLALHRLFCSREQV
                     LFQPADLPGDDQWAYFTADFILAPDEVALDRVPQARGVLTGPRGADTFHMVSRIFGEVD
                     PFFYPFVQFRGQVSGRDAVLLCSRKHIPLNSSTVSTLADAMRGSPLRLRGFGSVGHTAD
                     DVVEIQPIVLRTGSATS"
     gene            10001..10291
                     /locus_tag="AMYAL_RS50610"
     CDS             10001..10291
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Pantocin_Microcin_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /locus_tag="AMYAL_RS50610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="WP_245193414.1"
                     /sec_met_domain="Pantocin_Microcin_RRE (E-value: 1.4e-13,
                     bitscore: 49.7, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 6.8e-06, bitscore: 25.3,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIGGIVRCIAADVRDPDGWVWALLETLDGTRTVDQVVADLVRRF
                     PARSENENDVREALADFVEAGYVEDAFDPPPDRIDPVTSSSRRAHARRHLR"
     aSDomain        10004..10219
                     /aSDomain="Pantocin_Microcin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing E1-like protein in a
                     pantocin/microcin cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_AMYAL_RS50610_Pantocin_Microcin_RRE.1
                     "
                     /evalue="4.50E-16"
                     /identifier="RREFam017.1"
                     /label="AMYAL_RS50610"
                     /locus_tag="AMYAL_RS50610"
                     /protein_end="73"
                     /protein_start="1"
                     /score="49.7"
                     /tool="antismash"
                     /translation="RIGGIVRCIAADVRDPDGWVWALLETLDGTRTVDQVVADLVRRFP
                     ARSENENDVREALADFVEAGYVEDAFD"
     gene            complement(10245..11495)
                     /locus_tag="AMYAL_RS0138360"
     CDS             complement(10245..11495)
                     /GO_function="GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982841.1"
                     /locus_tag="AMYAL_RS0138360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="exonuclease domain-containing protein"
                     /protein_id="WP_020636607.1"
                     /transl_table=11
                     /translation="MTSLRGFAVVDTETTGIRPGYRHRIAEIAVVHVDFSGEVTDEWST
                     LVNPDRDLGPQAIHGIRAAEVRRAPKFEQFAGDVLELFRGRVPVAHNLPFDAMHLRAEF
                     ARLDVDLSRYLSGGVCTMRLAGDVFPGMRRSLVECCKAVGVDGVRFHTALGDALATAQL
                     FGRLVEGAPDLASFRLRGLPDVYGDLPSMPQGLVKPVHRQAAGHAEPHFLARLVDRVPR
                     EGKPEVDAYLAVLDRALLDRQISVSEADALIDVAHDLGLHKAEVIQLHHGYLRSLAQAA
                     WADGHVSVGERRDLEAVAALLGLVADEVDLVLREEQPTRVERSRSQVVEVNIGGLVLRP
                     GDRIVLTGTMRRQRGELTAEALALGLQVISSVSRKTRVVAAADPDSLSGKAKDARALGV
                     PVVTEDAFVRALDAMKR"
     misc_feature    complement(10335..10337)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(10674..10676)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(11492..12193)
                     /locus_tag="AMYAL_RS47195"
     CDS             complement(11492..12193)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039379.3"
                     /locus_tag="AMYAL_RS47195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6218 family protein"
                     /protein_id="WP_020636608.1"
                     /transl_table=11
                     /translation="MSDVDGMEGVVGGSVALSAPASAVVVIGPGVAGEESVAVWHVSPQ
                     GMPVGAWIYQLDRLLVSRDEARRLLTLVERRSIAAVVPGELDEVLDRVTKAAGVEAEKW
                     WTAQLFSPLQCFADIVSRRVAYDSTVSVAKRELKNVADLEWSRDLTAEQVVGFDDLRSL
                     SKVSAVVGSTVVGSAALTAVGVLRWLVRQWVETEGVKRRSYVRDAHGEAEPLPPSWLAS
                     VRAGMTTRLPL"
     gene            12370..12750
                     /locus_tag="AMYAL_RS0138370"
     CDS             12370..12750
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AMYAL_RS0138370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_020636609.1"
                     /transl_table=11
                     /translation="MRLPDGKQIVLSSDAAHLRSNIDQTTGMPLDVSNPDEENGLRKLK
                     LLASRPDTTVWVNHDPGRLETQPTQRTSDRLIPSAQQYAQAFGLSHFERLTHGYPVQRH
                     LETVVSPRLWHLPRAGRASNSR"
     gene            12793..13947
                     /locus_tag="AMYAL_RS0138375"
     CDS             12793..13947
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF023776.4"
                     /locus_tag="AMYAL_RS0138375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF3644 domain-containing protein"
                     /protein_id="WP_209447278.1"
                     /transl_table=11
                     /translation="MHMVGASRDEAQLAVRLYNDSSEVRSFEGFVVHMHLAWLYLLHAE
                     LTRDSIDFRYWRTRGRARRLERVDGEPKRWDLATCVRHRWPDKAAAVRANLEFFVALRN
                     KIEHRYAREQQALAVAVSGQSQAFLLNYEQELTGQFGTGSSLATRLRFPVFVGSFTDEG
                     ERTLRRLRAQLPVPLRTFIADYDAGLDASVAQDPRYDLRLRVLQELAPKDPDALAIQFT
                     RYDDLTDEQRAAVEAIGRKGYVVVREQKRPVVGHGLLRPTQAAQAVQDRIPFVFNTNHF
                     TTAWKKIGVRPKVGDPHPERTEEKYCLYDEPHRDYGYTEAYIAKLARDCATEAGFRALL
                     GTAPRDKVTGDWVGSPPLNATPPWRRSAPSAAQEPPDMDTMRSA"
     misc_feature    13393..13395
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    13807..13809
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    14237..14248
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    14522..14542
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.38"
                     /tool="antismash"
     misc_feature    complement(14795..14809)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 20.0"
                     /tool="antismash"
     gene            14803..16179
                     /locus_tag="AMYAL_RS0138380"
     CDS             14803..16179
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437904.1"
                     /locus_tag="AMYAL_RS0138380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_051137735.1"
                     /transl_table=11
                     /translation="MLKLAKHVLRDLAQRSNVSIHSDSSLVGAVAAAAADDDERRAKKR
                     DDESSAKDDDEVLRRKPELHPIRAWGDKSSQLTPWDVLHVLGRAIALSRRGAARGLAEH
                     WGALKYSQALTGDTGSFLKLSAEGKETADYYKAVQSSELGVGFALVLARRILTQRYPDR
                     VISIVPADTALRAGWALTSRDKGQRQNYRYRPQFFAELWKPEAPSLVLPIACKGNHSNA
                     AHSHVQLASASAHVEAVHVGPWNETPCLIFSTELPLDGHVTVHALCSEGSGGWLATPSA
                     EGSDQDAELSDVNHFPQIHLPSNGDEARISIPGFHITPDHYEWFGRVLARTAAAGLTAF
                     AGNGEATAQYLTERQGRKHFIGFTHAATGSVQDANHTLLGKRFVGTDHVFRLNGTRVEA
                     FSGVGAALFDLLAHGRVTQYRHKIYEHRSAWPSGSWDSSWGGPVSIHPDGTVLAIRLLP
                     "
     misc_feature    16021..16023
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(16226..16240)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     misc_feature    16264..16277
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.51"
                     /tool="antismash"
     misc_feature    complement(17261..17279)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 20.19"
                     /tool="antismash"
     gene            17609..17968
                     /locus_tag="AMYAL_RS47200"
     CDS             17609..17968
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AMYAL_RS47200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_020636613.1"
                     /transl_table=11
                     /translation="MESGPDLLIGAVRIDRTGRVNERLLFRALGWNPGQRLSLDMLHGL
                     IVIAPEPSGPHAVDARGALTLPAAARHLCGIEPDRPLVLAASIHEQLLIVHPGTTVAHL
                     LTGHYREITGLDHDA"
     misc_feature    complement(20174..20189)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.28"
                     /tool="antismash"
ORIGIN
        1 tcgacgtact tctcgtaggt ctggccgccg cggggaccca tgacctgctt cttgagcacc
       61 gccacgttct tgacgtcggt gtacttgagc aggccctggt ccccgtggcg ggcgccgacg
      121 cccgagttct tgacgccgcc ggacggcgtc cccttcgcgg cgtacgcggt ggccaggatg
      181 tcgttgatgt tgacgttgcc ggactcgatc cgcgaggcca cggcgcgggc ggcgtcgacg
      241 tcaccgcccc agaccgaggc attgaggccg tattcggtgt cgttggccag cgcgacggcc
      301 tcgtcgaccg tgacgtactt gtgcagcgcg acaacgggtc cgaaggtttc ggtgacaccg
      361 cagagcatgt ccttcgtgac gccttcgaga atcgtcggct cgaagaacgc cgggccgaga
      421 tcgggccggg gctttcctcc gcaaaggacg gtggcgccct tcgcgacggc gtcctcgacg
      481 tgtgacttga cgcggttcat gtggtcgacg gagacgagcg aacccatgtc gggtccgaag
      541 tcgtaggcgg cgcggacgtc gagagccttg gtcttggcca cgtacgcgtt cttgaactcg
      601 tcgtagcggg actccggcag gtagatccgc tcgatgtgca tgcagatctg cccggtgttg
      661 ccgaaagcac cgaagatcgc gccctgtgcg gcctcggcca ggtcggcgtc ttcgagcacg
      721 atcatcgggt tcttgccccc gagttcgaga cagcagccga tgaggttgcg gcccgcccgc
      781 tcgccgatca cccggccggt ggccgtggaa ccggtgaaca tcacgtagtt cgcgtggtcg
      841 atgagcgtgg gcccgacgtc cgggccctca ccgcacacca cctggaacag ccccttcggc
      901 aatccggcct gctccagcag ctcgacgccg tacagcgggg agagcgccgt cttgttgtcg
      961 ggcttcagca cgaccgcgtt cccggccatc agcgccggga tggcgtcgga aatgccggtg
     1021 gcgaaaggga agttccaggg cgcgatgatg ccgacgaccc ccttgggctg ccggatctcg
     1081 gtcgacgtcg tcacgaacgg gaccggtccc ccgcgtttgg tcggtgccag cagtttcgcg
     1141 gccctcgtga ggtaatggct catgaccatc gccgggtcac aggtctcctc gatggccatc
     1201 cggcggttct tgccgctctc gacctggatg aggtcggtga cgatcggcgc gttgtcgacg
     1261 aagagcgtgt gcgcccgctt gaacacctcc aggcgcttct tgagcggcgt ggcagcccac
     1321 ttcgcttgcg cggagcgcgc cgtggtgaat gcctgctcga tgtcggcggg tgtcgactgg
     1381 ggcagctcga cgagcacgtc gccggtgtag acctcggcga gcttccaggt cgcgccggac
     1441 gagcccggca cacgggcggc gaggcggcgc aggaacgcgt cgatcaccga cgccggacgg
     1501 gtgagcgcga gcggtgtgac ggtcatggcc ctctttccgt cagttgtccg agaggacgag
     1561 ctgcgcgccc atctcaccga tcatgatggc gggcgcgttg gtgttgccgc cggtgatcga
     1621 cggcatgatc gaggcgtcgc agacccgcag tccctcgacg ccgcggacct tgaggtcggg
     1681 accgacgaca gcgagttcgt cgacgcccat ccggcaggtg ccgacgccgt ggtagaccga
     1741 ggtcgcgcgg ttcaggatcg cgtcgcgcag ttcctggccc tgaagcgcct tgccagggtg
     1801 gagctcttcc ttgatcgagc cgttgaacgc cttcgaagag aagatctcgc ggaccatctc
     1861 cgagccctcg ccgagcactt cgaggtctgc cggatcggac aggtactgga agtcgatgag
     1921 cggcgccgcc gtgggatcgg ccgaggcgag ccgcagcgtg ccgcggctct tcgggtagat
     1981 cagcgtggtc agcacggtga gcgcgggccg cttgtcgacg tcgtgccgga tcggggcgtc
     2041 ctggttgggc gtcacgtacg cccacggcag gaggtgcagc tggaggtcgg ggatcgtggt
     2101 ggcctgggag gtcttgagga acgcgaccgc ttcgaagacc gagttcgcca ggaaggtcgt
     2161 gcccggccgc ataagctcct tcacgaggcc ggtggcgaag tagggcggcg tgcccttgtt
     2221 cttgctcgac gacgcgcgga aggtcagcgc gtggaacatg tggtcgtgca ggttgtcgcc
     2281 gaccggcagg tccgcgacga cgtcgatgcc gtgttccttc aggtgttcgg cgtgaccgat
     2341 cccggacagc atcagcagtt gcgcggagcc gacgaagccc gccgagagga tgacctcctt
     2401 gcccgcgcgc agcgtccgcc tgctcccgtc cgcgtcgacg acctcgacgc cgaccgcgcg
     2461 gccattctcg acgaggacct tcttcgccag cacaccggac tggacctgca gcgtggcggg
     2521 cgcgagctcg tggatgtacc cgcgcgaggc gctgtagcgc aggccgtcgg cggcattctg
     2581 ctgcatcctg ctgacgccct cttgcgactc ggcgttgtag tcgtcgagga tctcgcagcc
     2641 gatcgcgtcg gcggtcgcct ggacgaactg cagcgtgccc tcctgcggga tcttgttgcg
     2701 ggtgacccgg atcggcccgc ccttgccccg gaactcgctc tcgccgtcct cgaaatcctc
     2761 catccgcttg tacgcggcgt tgacgctgtc ggcgtcccag cccttgttgc cctcggccgc
     2821 ccaggagtcg aagttggcgc ggttgccgcg cacgtagacc atgccgttga tggagctgga
     2881 gccgccgacc accttgccac gcggcaccgg catccggcga tcgagaacgt gtttctgcgg
     2941 caccgagtag tagccccagt cgaagggttt cttgagctgg ggcaccgcgt gcatcgggcc
     3001 gaccagtccc ggtttcctga cgagcatccg ctcgtcgctc ttgcctgctt cgagcacgat
     3061 cacgctggct ccggactccg ccagcctgcc ggcgatcgcg gcacccgagc tgcccgatcc
     3121 gacgaccacg tagtcggcct cgtcgccgcg cgcagccctg ttcgccattt ccgctcctcg
     3181 gtccgcccgc cgtgcagaac tataacctgt tctagttccg tcaccgtata gcgagaccgc
     3241 ccggttacgc aatggcgcgt ctcgccgcac gtgcgggccg cctagtcgca gagtcgaaaa
     3301 cttgacccat tccgattatc tgactcgtca gacgcgagat caacttcctg cggaatccgt
     3361 agtacaaaac ctcctgaaca gccatttccg gcaacttgca cactttccgt agtcacgacc
     3421 attgccagcg tgatcgcaca tctctaaagt gattaatcgc aaacgcttcg acagggaggg
     3481 tcgacggatg cggggggtcg tacttgtggc ctcggcgggc actgccgtgg cctacgtcgt
     3541 gctgacggtg gaaatctggg ccttgctctt cggcttcagc acctgcgccg ccttggtgct
     3601 ggcgtgtctc gcgcggagga gatgactcct gaacgcgcga acggcctgca cgcggacgga
     3661 tgggtccgcg tgcaggccgg cctgttcact cagcgcagcg gttcgagagc cttcgccagc
     3721 ggctccagaa cggcgggggt gtgcggcggc ggcaggtaga tgatcgccag gtcgagaccc
     3781 gcttcaccga gggcttcggc gtccgcggcg accttggcgt agtcgccgtc ggcgctgagg
     3841 cggacatgcg aggacagcgt gatctcgctc gggtcccggc cgatgtccgc gcagtgccgg
     3901 tgcaggactt cacgtttgtg cgcgaactct tccggcgtac cgccgacgaa gttccagtgc
     3961 tgggcgtact tcgcggccgt gcgcaaggtc cgcttctcgc cgctgccacc gatgcagatc
     4021 ggcggatgcg gcttctgcac gcccttcggc tcgcaacggg cgtcggtcag ctggtagtgc
     4081 tccccctgga aagtggtggt ctcctgggtc agcagcccga cgaggacctc gcagccttcc
     4141 tcgaaccggt cactgcgctc cttgatcgtg cccagttcca tgccgtacgc gccggactcc
     4201 tcttcgttcc agcccgcacc gatcccgatc tccaggcggc cgccggaaac gatgtcgagc
     4261 gtcgcggcca tgttcgcgag cagcgcgggg tggcggtagt ggatcccgct gaccagcgtg
     4321 cccagccgga gccgtttcgt ggcctgggcg agcgcggtga gggtcaccca cccttccagg
     4381 cacggaccgg tcgaatcaga gaaaatggga tagaaatggt cgaaggtcca gccggattcg
     4441 aagagttcga tctcgtcggc ggcctgccat accgcgagca tgtcggacca aacggtgtcc
     4501 tggggcgaag tcttgatggc gaatcgcatg attctgatcg taaacctgga gttcctcgag
     4561 catcggtcac cgatcggggt cggcgggccc ccgatcaggt cccccgcccg caccgccggg
     4621 tggacgtgac cgtgaggcct atcggagatg tcgatcttcg tcctaccgtc gaagaatccc
     4681 gactctccga aaggactcgt tcatgcgcgc acgtagcgct ttcctcgctg tcacaggcgt
     4741 tgtcgccctg ttcctcacga tcccggcgac ggcttccgcg gccgaaggcg aattcaccta
     4801 ccgctactac gacgaacacg aagagatcca ggtgggcgtc ctggtggatc cgcccagcgg
     4861 cgagtgcatc gaaatccccg aaatcgccga ctgggacgtc gacgcgttcc gcccgcgcaa
     4921 cgacaccgac tcgacggcgg ttgtgttcat ggacgacgac tgctcgagcg acgcctactt
     4981 ttccctacgc cccaacggtg gccgcggctc ggacagactc ctgctgcgct cggtgatctt
     5041 cagctagtcc cggccaggat ggtcagcccg taggccgaca ggacccggtt gaccgggagg
     5101 aagtacgtgg tgcctccggt ggaacagttc ccgctgccgc ccaccagcac tccctgcgcc
     5161 tgcgttccgc tgacgaacgg ccccccggcg tcgccaggct cggcgcagac ggaggtcctg
     5221 gtcagtccgg tcaacgttcc ccctgggaag gtgatcgtct ggttcttccc ctggatggtt
     5281 ccgcaatgcc agccggtggt cgagccgtac ctgcacaccg acgcgccgac cggggcttcg
     5341 gccgagccgg tgatcgtcac ccggctgtcg acgggaggaa tggatcccac cagccgccag
     5401 tcggtcgtgt tggtcacccg gatgacgatc gcaccggtcg gcggaacctg gtccgccacg
     5461 accaccccga cgacacgtcc cgcgctgcgg accgtgtcgc cgggcctgcc gcaggcggaa
     5521 ctcgcgatca ggtagccggt attacctgcg acagccgcga atcccgccgt gcaccggctc
     5581 tgcagcacgc tcccgccgcc gagcggggcc ggagcgtccg cttgcgcggt cggcggccag
     5641 gccacccaga gcacaaggac caggccgagc aggaaacggg atcggggcag cctcatggga
     5701 ccctccgatg gccggggatt gccccgattc taacccgttc agcgccagcc gagctccgga
     5761 gccacgtgcg tgagaatgct ttccagcacg tgcgcgttgt actcgacacc gagctgattc
     5821 ggaatggtca gcagcaaggt gtccgcggcc tggatcgcct cgtcggcctt gagctcccgc
     5881 accagctcgt ccggctcggc cgcgtacgac cgcccgaaga tcgccctggt cgtctcgtcg
     5941 atcatgccga cctggtcacg cgagtcacgg tcgcggccga agtaggcgcg gtcgaggtcg
     6001 ctggtcaacg cgaagatgct ccggctgacc gacacccgcg gctcacgttc gtgaccggcc
     6061 tctttccacg cttcgcgata ggcctcgatc tgcttacgct gctggacgtg cagcggctcc
     6121 cccgtctcgt cgtccttgag cgtggaactc tgcagattca tgcccagctc cgcggcccag
     6181 accgcggtcg cgttcgaact ggatccccac cagatccggt cgcgcagccc ctcggagtac
     6241 ggctcgagcc ggagcagccc cggcggattg gcgaacatcg gacgcgggtt cggctgcgcg
     6301 aagccctccc cgtcgagcac cttgaggaag acctcggtcc gctgccgggc catgtccgcg
     6361 gccgtctccc cctccgcggg cccgtagccg aagtaacgcc agccgtcgat cacctgctcc
     6421 ggggatcccc ggctgacacc gagctggagc ctgccaccgg aaatgaggtc tgcggcgccc
     6481 gcgtcctcgg ccatgtacat cgggttctcg tagcgcatgt cgatcacgcc ggtcccgatc
     6541 tcgatcttcg aggtccgcgc cccgatcgcc gacagcaacg ggaacggact acccgcctga
     6601 cgcgcgaagt ggtgcacacg gaagtacgcg ccgtccacgc cgagctcctc ggccgcgacg
     6661 gccaggtcga tcgactgaag cagaaagtcg gcggcggacc gggtctcgga gtgcgcactc
     6721 gccgaccagt ggccgaacga gagaaaacca atcttcttca cgcccttgta agcgtccgat
     6781 gaccgggttt gattcccccg ccgcccactt cacagagcca gccctcgacg actctacgtg
     6841 actagctgtc acgttgcgcg tttccggctc tcgacctgca gccttgcgtg cagcgttcgc
     6901 ccacatggac gcctgagtca tgccgcaggg taaccgctca tcctctcaat gtgcgtctcc
     6961 gtgaccagac gtacccccct gattcaggcc tccaggcggg gtatagtctc ttctcatcag
     7021 caagtccgag tggcggaatg gcagacgcgc tagcttgagg tgctagtgtc cttaacggac
     7081 gtgggggttc aagtcccccc tcggacacac gcattaaccc tacggggtgg gcggatggtc
     7141 acatcgaccg tccgcccttt tgcgttatag accatctcca ggccgatctc gccgtagagc
     7201 gcctgcagtc tccccggact cgcgttcccg acctgccttc ccagttcacc gaacgcatcg
     7261 accatcgctt cgagttcagc tctgccgatc gactcctcag ccggagcacg cgccagatcc
     7321 tcctcggcgg cgtcgcgctc ctccttcgcc cggttcggtg aaccccgaag tccgcgtgga
     7381 cagcaccacg cagacccctg ccgaatcggc ctcggtgatc ctggccaaac ttcgcgaaac
     7441 gggcgtctag cgtccggtct ggccggggtt caggaagaca gctccctgaa cccggcgatc
     7501 agcggccgag cgcttgccgc acagcccgca attcggcaag gtattcggcc gccgagtctg
     7561 gatcattcct caggtgcgct tcggcgaggt cacgcagtgt gtcgtgatac tcggccagtt
     7621 caccgagtcg cagccatatc gcccgagcgt tctccaatga ggcagtgact tccgcgagcg
     7681 ggtgtcctgt gagtaacagg gtgcggccgc gccagccgtc ggccatcgcc tcgcaccagt
     7741 tagcgtggtc tgggaagtgg gtgcctgccg cagcgaaaag gtcgcaggca gccacgaggt
     7801 ctggtgcgtg gcgcttcggg cgtcgcaccc gatcagacac cttttcgcct gctcgccggt
     7861 ggtacatggt cgcgacggcc gcgaccacgc cgggcaggtg ctcgggaacc aggcggtcac
     7921 cgagttcctg cgtgcgggcc agccaggaca tctcctgctc gctgatgaac ccctcgaaca
     7981 acaacccagc cagcatgacc agagggttgg ctgacaccgg cacgtaactc tccgacgcac
     8041 gccaacgtac gatcgccgct tcggcctcgg ccaaggcaca ctccagattt tcccggtgct
     8101 gttcgacgat gaggtcctcg tcgagcagtt cggtggcgcg gtgaacgagg ttgtcgtcga
     8161 tccattcgcg taccggcgaa tcgtccagtg gcaccggtcc gtcggcgaag gccatggtca
     8221 ggatgctgat ggaggcatcg atgatctctc ggtagctgtc gatccggaac accaggtgcg
     8281 ggtcggcaac tttggccagc atctcgctct tgagcatctc cagccggtcc gggggcgaca
     8341 tggccaacgg gtgctcggga tacagctcgg tgtgcacggc gaagctgtga tgcaaggtgt
     8401 ccatctcgaa atacggcgcg gtcttgggca gcttcagcac gacgtcgtct tgtgtcggct
     8461 cgctcagcgg cgcgatcgaa tacaccactg attccgcgcc ctcaccgacg aatttggtga
     8521 gcagtaccgg ttgctccccg tccggccagt tgcacgcacc ggtcatgtcc aacttgagca
     8581 ccgtgccgac caggttgtcc cgcagcacct cagctcgtcg ccgatccggt ccggagcacg
     8641 atcggctgga tctcgaccac gtcgtcggcc gtgtggccga cagaaccgaa gccgcgcaac
     8701 cgcaggggcg atccacgcat cgcgtcggcg agcgtggaga cagtgcttga gttcaacggg
     8761 atgtgcttac gcgagcacaa cagcaccgcg tcgcgaccgc tgacctggcc gcggaactgc
     8821 acgaagggat agaagaacgg gtcgacctcg ccgaagatcc tgctcaccat gtgaaaagtg
     8881 tccgccccgc gcgggccggt caggactccc ctcgcctggg gaacgcgatc gagcgcgact
     8941 tcatccggtg ccaggatgaa gtcggcggtg aagtaggccc actgatcgtc gccaggcagg
     9001 tcggcgggct gaaacaaaac ctgctcacgg gaacagaaca gacgatgcag cgcgaggaag
     9061 cgctgttctg gctcctcgat gtccgggaga aggtggcggt acgcccggtg aagggtggcc
     9121 accttgcgag tgctgaagta gaaccgctgg gccagcagcc gggcaggctc cgcgaacgcc
     9181 gtgatgtcgc cgaccagcgt cgcgagtcgt ggttcggaca agaccggcag gtgggaattg
     9241 cggtcctccc ggaaggccag cagcactgac cgggtgatct cctcggcatt ttcccgagga
     9301 tcggcgacgc cgagtgagtc cagtcgcgct tcgagatcgt gcagggcgat ttccaacgcc
     9361 gccgccgcag cttcgctcaa ctcgccgcac cggacgaccc cgtcccgatc aggaacggtg
     9421 aaatggaagt ggaccccgcc ttcgatgtcg cgcgcctgca cggtgaatcc cgagatcccg
     9481 gacgccacgt tcaccagttc ggtcacagca atcacctcgc ggcgacatta gcagtcagcg
     9541 attgctcagg cgggaactct cgaccacgaa gacggcaagt accggccaaa ggctccgctg
     9601 agggccgggc caggtgcccc cgctgacggt tttcacccgt ctggatgcgt tccggccgct
     9661 ccgtgcaccg gctcttgtcc cggtttccgt ccggttcgaa cggtcatgct ctgcgctgtc
     9721 aacgatcgag agcgtggagg gaacggcatg gatccgctgg agtttcacac acagccgtcc
     9781 gatgcggacg cgccgcgaac aggctgtgtc acggccaccc ggttcctccg ccgagcgcag
     9841 acgaggggag gtgcgccgcg atgatcgagt tcttgaaggt ggagcgggag atgacgttca
     9901 gccagaccga cgacaaagag aattccggcg aagggagggt tccatgcgtc gccctaagat
     9961 caaatctgga caccagccct tccgccatgg cgaggacctg atgcgaatcg gcggcatcgt
    10021 gcgctgtatc gccgcagacg tccgggatcc ggacgggtgg gtgtgggcgc tgctggaaac
    10081 cctcgacggg acgcgaacgg tggatcaggt ggtcgccgac ctggtccgcc ggttccctgc
    10141 gcgctctgag aatgagaatg acgtacgcga agcgctcgct gatttcgtcg aggccggcta
    10201 tgtcgaggac gcgttcgacc cacctcctga ccgaatcgat ccagttacct cttcatcgcg
    10261 tcgagcgcac gcacgaaggc atcttcggta acaacgggaa ctcctagagc ccgcgcgtcc
    10321 tttgctttgc cggataacga atccgggtcc gcggcggcaa cgactcgggt cttcctgctc
    10381 actgagctga tcacctgaag gccgagggcc agcgcttctg cggtgagttc gccgcgctga
    10441 cggcgcatcg ttccggtcag aacgatccgg tcaccagggc gcagcacaag ccctccgata
    10501 ttgacctcta cgacctggga ccgagatcgt tccacccgcg tgggctgttc ctcacggaga
    10561 acgagatcga cctcatcggc aacaaggccg agaagggcag ctaccgcttc cagatctcgt
    10621 cgctctccca cggacacgtg cccgtccgcc caagcggctt gcgcgaggct gcgtaagtag
    10681 ccgtggtgca actggatgac ctccgccttg tgcaggccga ggtcatgagc gacatcgatc
    10741 agcgcatcgg cttccgaaac cgagatctgc cgatcaagca atgctctatc caggacggcg
    10801 aggtacgcgt cgacctcggg cttcccctca cgagggacac gatccaccaa ccgggcaagg
    10861 aagtgcggct cggcgtgacc ggccgcctgc cggtgtaccg gcttgaccag accttgaggc
    10921 atggaaggca ggtccccata cacatccggc aagccacgaa gccgaaatga ggctaggtcc
    10981 ggggcgcctt ctaccagtcg accgaacagc tgggccgtgg caagggcatc accgagcgca
    11041 gtatggaacc gcacgccgtc cacgccgaca gccttgcagc actcgacgag cgaccttcgc
    11101 atcccaggga aaacgtcgcc tgcgagcctc atggtgcaca cgccaccgga caggtaccgg
    11161 gacaggtcca cgtccaggcg ggcgaattcg gcacgcaggt gcatggcgtc gaacggcagg
    11221 ttgtgcgcga caggaacacg acctcggaag agctcgagca cgtcgccggc gaactgctcg
    11281 aacttcggag cacgacgcac ctcagcggcg cggatgccat ggatggcctg agggccgaga
    11341 tcacggtccg ggttcaccag ggtcgaccac tcgtcggtga cctcgccgga gaaatcgaca
    11401 tggacgaccg cgatctcggc gattcgatgt cggtaaccag gccgtatccc cgttgtctcg
    11461 gtgtcgacga ccgcgaaccc acgaagcgac gtcatagcgg cagccgagta gtcatgcctg
    11521 cccgcaccga cgcaagccag gacggtggca acggctctgc ttccccgtgc gcgtcccgga
    11581 cgtagcttcg cctcttcacg ccttcggtct cgacccattg ccggaccagc catcgcagta
    11641 ccccgaccgc cgtcaacgcg gcggaaccga ccactgtcga tcccacgacg gcagacacct
    11701 tcgacaagga ccgtaggtca tcgaatccga cgacctgctc ggctgtgagg tcgcggctcc
    11761 attccaggtc cgctacgttc ttgagctcac gcttcgccac cgacacggtg ctgtcgtacg
    11821 ccacccgacg gctaacgata tccgcgaaac actgaagcgg cgaaaacaac tgcgctgtcc
    11881 accacttctc cgcctcgaca ccagcggcct tcgtgactct gtcgagcact tcatccagct
    11941 ctcccggaac gaccgcggcg atcgaccgtc gctccaccaa ggtcaacaag cgacgcgcct
    12001 cgtcccgcga caccagcaaa cgatcgagct ggtagatcca cgctccgacc ggcatccctt
    12061 gcggactcac atgccagacc gcgactgact cctctcctgc cactccagga ccgatcacca
    12121 ccacagcgct cgcaggagcc gacaaggcga ccgagccccc taccacgcct tccatcccgt
    12181 caacgtcgct cacacgccac ccaccctcga atcagtcgaa cgactcaaca agtcgtagag
    12241 cgggtcaacc tgttacgcga gcttctccga ccgaaacaaa actgtggccg taggtccagc
    12301 acctagtggg cagcgacccg gcgcttcgtc cgcgccgagt ccctgcccgg acacaccctc
    12361 tgactccacg tgcggttgcc cgacggcaag cagatcgtcc tcagcagcga cgccgcccac
    12421 ctgcgcagca acatcgacca gaccactggc atgccgctgg acgtctccaa ccctgacgag
    12481 gagaacggtc tgcgcaagct gaaactgctc gccagcaggc cggacaccac ggtctgggtc
    12541 aaccacgacc ccggacgact ggaaactcaa ccgacccagc ggacgtcaga tcgcctgatc
    12601 ccgtcggctc agcagtacgc tcaggcgttc ggcttgagcc acttcgaacg tcttacccac
    12661 ggttatcccg tccagcgtca tctcgagacc gtggtctcgc cccgcctttg gcatctgcca
    12721 cgagcaggac gggccagtaa cagtcggtga caagatcccg accttcatct catgccaccg
    12781 cgaccacgat ttatgcacat ggtcggcgct tctcgcgatg aagcgcagct tgccgtgcgc
    12841 ttgtacaacg actcgtcgga ggttcgttcc ttcgagggct tcgtagtcca catgcacctt
    12901 gcctggcttt acctcctgca cgccgaactc actcgggaca gcatcgactt ccggtactgg
    12961 cgcactcggg gacgagcacg gcggctggaa cgggtcgacg gtgaaccgaa gcgctgggat
    13021 ctggccactt gcgtgcggca tcgctggccc gacaaggccg ccgcggtgcg ggccaatctc
    13081 gagttcttcg ttgcgctgcg caacaagatc gagcaccggt acgcccgcga gcagcaagca
    13141 ctcgccgtcg ccgtgagcgg ccagagccag gcgttcctgc tcaactacga gcaggaactc
    13201 actggccaat tcggaaccgg atcgtccctc gccacccgcc tgcggttccc ggtcttcgtc
    13261 ggaagcttca ccgacgaggg cgaacgcacg ctgcggcggc tgcgcgcaca gctacccgta
    13321 ccgctgcgaa ccttcatcgc cgactacgac gccggtctcg acgcatccgt cgcccaggac
    13381 cctcggtacg acttacggct gcgagtgctc caggaactcg cgcccaagga cccggacgcg
    13441 ctcgcgatcc agttcacccg ctatgacgat ctgaccgacg aacagcgggc cgccgtcgag
    13501 gcgatcggca gaaaaggcta cgtagtcgtc cgcgaacaga aacgccccgt cgtcggacac
    13561 gggttgctcc ggccgacaca ggccgcgcaa gcagttcaag accggatccc gttcgtattc
    13621 aacaccaacc atttcacaac cgcctggaaa aagatcggtg tccggccgaa ggtgggagat
    13681 ccgcatcccg agaggaccga agaaaagtac tgcctctacg acgagccaca ccgcgactac
    13741 ggctacaccg aagcctacat cgccaagctt gcccgcgact gcgccaccga agcaggcttc
    13801 cgcgccttac tggggaccgc cccgagagac aaagtgaccg gggactgggt gggatcgcca
    13861 ccactcaacg cgacacctcc ctggagacga tcagccccgt cagcagcaca agaacctccg
    13921 gacatggaca cgatgcgctc agcctgacat ctccgaagac aaacatcgcg ctgcaccgtc
    13981 acagacgggt gattcagaca tcgcaggcgt tgcgcggacc gaaccaaacg agaccatggc
    14041 atggtgggac gacgcgtccc tgggaccgca tgagccgtcc ggccgcgaga gcagttgatc
    14101 gcggaactgg cgccagacag catccagcgc aactcccaac ctgcccggct accggcgttg
    14161 cccgagccgg gcctgccggt ggaatcaggc cccgatctgc tgatcgcacc gtacgcattg
    14221 accggaccgg ccccgcgtca acgaacgcct cctcttacgc gcgctctgct ggcacccagg
    14281 ccgccggctc tcactggaca cgctgcgcgg gctgatcgtg gtcgcacccg acacatccgg
    14341 ccagcacgtg ggctctctcg cgatcgaacg acaggctccg tagacggtct ggccgtggtc
    14401 ggtcgccacc ttcaccccca ggcagcggac aaacctcggc taccggcgga cggcgacctg
    14461 ccggaacgcg agtttgtagc tggattcacc tgctcgtggc cgctcgacgg acacgtcggc
    14521 gacaaggtcc tgatgacgat gaacgtggcc atgcccacta cgagaaccgc gcagaacgcg
    14581 atgacgatca ccagttttcg tttccggacc cgagacggag gcacgctcgg cacgacggtt
    14641 gggcaaggtt caaactctgg tcgtcgacta cgccccaaga tcatcgacat acctcactcc
    14701 ttgagcggaa ccgcgcccgt gatcaagaac gtatcgttgt gtgtcggtgt tgatcacgcg
    14761 gcagtgcgac caggtcgacg tagagggcgc ataaggaaaa tgatgttgaa gctggcgaag
    14821 cacgtgctaa gggatctcgc gcaacgctcg aacgtctcca tccattcgga ttccagtttg
    14881 gttggcgccg tggctgccgc cgcagccgac gacgacgaaa ggcgcgcaaa gaagcgggat
    14941 gatgagagca gcgctaaaga cgacgacgaa gtcctacggc ggaagcctga actccatccg
    15001 atacgcgcat ggggagacaa atcctcgcag ctgacaccgt gggacgtgct gcatgtactc
    15061 ggccgagcca tcgcactgtc cagacgaggc gccgcgcgcg gactcgccga gcactggggc
    15121 gcactgaagt acagccaggc gctgaccggc gacacaggtt ccttcctgaa gctatccgct
    15181 gagggcaagg agacggcgga ctactacaag gccgtccagt cgagcgagct cggtgtcggc
    15241 ttcgcactgg tactggccag gcggatcctg acgcaacgat atcctgatcg cgtcatctcc
    15301 atcgtgcccg cggacacggc actgcgcgca ggctgggcct tgaccagcag agacaaggga
    15361 caacgacaga actaccggta ccgcccacag ttcttcgccg agctatggaa gccggaagcg
    15421 ccttcgctag tactgccgat cgcctgcaag ggcaaccaca gcaacgccgc acactcccac
    15481 gtccaactcg cgtctgcatc ggctcacgtc gaggcggtgc acgtcggacc atggaacgag
    15541 accccctgcc tgatcttcag cactgagctg cccctggacg gacacgtcac cgtgcacgcc
    15601 ctctgctccg aaggaagcgg cggctggctg gccactccca gcgctgaggg tagcgatcag
    15661 gatgccgagc tcagcgacgt caaccacttc ccccaaatcc acctgccgtc caacggtgac
    15721 gaagcccgta tctcgatccc cggcttccat atcacaccgg atcactacga atggttcggg
    15781 cgcgtgctcg cacgaacggc cgccgcggga ctgacagcct tcgccggcaa tggcgaagcg
    15841 accgcccagt acctgaccga gcgccaggga cggaagcact tcattggctt cacgcacgcg
    15901 gccactggta gcgtgcagga cgcgaaccac accctcctcg gcaaacgctt cgtcggcacc
    15961 gaccacgtct tccggctgaa cggcacccgc gtggaagcct tctctggcgt aggtgccgca
    16021 ttattcgatc tcctggctca cggacgagtg acgcagtacc gccacaagat ctacgagcac
    16081 cgctccgcct ggccgagcgg ttcctgggac agctcatggg gcggcccggt atcaattcac
    16141 ccggatggaa ccgtactcgc catacgcctg ctcccctgac gtgacaatcc gtcgcggaga
    16201 cgcgaggccg accgcatcaa cttcttggcc gatcccgacc tccgcgtcct cggagatgat
    16261 tactggacct tgtccagttc ctgtccgcct ggtcgcggcg taatgccagg ctttccggat
    16321 tcctcttgct gccagcgatg tccggaaatg tgcgcgacga actcacgagt atacttaacg
    16381 ttaatccccg tctacgcgtc gtctcgccaa catcgtgctc ccctgatcaa ccattccgat
    16441 catcggacac gccctggctg cactaaccgc tccgtccgac gttcgtcaag caaccgtcga
    16501 gcgttcgcct gactgaaggg caacttggcg tctccatgcg gcctgatccg gctccactaa
    16561 gcactgcatc tggcgggcga agcccagcga caagcccgat tcgccgatga cttcagccag
    16621 cgatcaggtc tctgatgaac gcggcgatcg ctacgaacat actcgcccca gcgatggaca
    16681 tcatcgccca gtcgtaaccc ggcctgtccg ccctcgaaga catggctgcc gctccgcgga
    16741 cagccgagct tggttgccgc tctggcaagg caccacgatc gaaggtgtca cgcggccaca
    16801 tcaaggtcgg aagcccaaga aaacccctac cgcggacaag aacaggaagc ccgcgcttgt
    16861 aaccgacgtg gaagtcgcac gtgccggcga taccccgaac aagccagaga cacggccgat
    16921 gcttgaccgt ctcgactctg caacgagtca gcctaactac ggtcaagtag agcagcaagg
    16981 ctgcgactgc gaaaagcaaa ggccgccaca gccgttccga ataaaaatag tatgccagga
    17041 ccgcgagaac tactccccaa gccctgaacg gagtcttacg acgacgtcct tttgccacgg
    17101 atacctcccc acatcgatcg tggcggtcgc ctatgcgggg aaacgaccgc cgctcgagtg
    17161 acggggtagc actagtcgtc ctcctaatac gacacatgac gtggctatga gctaatcctg
    17221 agactggtgt tatcacctgt gaagcatgcg ggtgacagaa gctcgatgtg tatccagcca
    17281 aatttgaggc ttgatgacac atgagcggcg actattgaca catggaccac catggcacga
    17341 ctgccgccga cgagtgcaac gagtagaggc ttcctctagc tcgaggttca catagcccct
    17401 ccccatcctc acgaacgggt ggttctgggg tcgcggggtt tgcgtcgatc actccggaca
    17461 ccaccatggg atggtgggac aacgcgtccc tgggaccgca catgcagtcc ggccccagga
    17521 gcagttgatc gcggcactgg tgcccgacag cacccagcgc catccccaac ctgcccggct
    17581 accgactctg cccgagccgc gtctgccggt ggaatcaggc cctgacctgc tgatcggcgc
    17641 cgtccgcatc gaccggaccg gccgcgtcaa cgaacgtctc ctattccgcg cactcggctg
    17701 gaacccaggc cagcgacttt cactggacat gctgcacggg ctgatcgtga tcgcacccga
    17761 accctccggc ccgcacgccg tcgatgcccg cggagctctc acccttcccg ccgcggcccg
    17821 ccacctgtgc ggcatcgaac ccgaccggcc cctcgtcctc gccgcctcga tccacgaaca
    17881 actgctcatc gtgcaccccg gcacgaccgt cgcccacctc ctgaccgggc actaccgcga
    17941 gataactggg ctggatcatg acgcctgacc ccgcccagat ccgcgccgcc ccccaaacac
    18001 tggccgaact gggcgttacc ctcgcagacc tccaggacga cccagccgag acacccggta
    18061 tccccaccct ggccggctac ctcccccatg tcatcgccgc cgcaggcgcc ggagccagcc
    18121 gcacctacgg caactactgg acacgcatgg ccacagcctg gggcgaccgg cgcctcgacc
    18181 agatcaccgc caccgacatc gaggcgatgc aacacgacgc cgccacccac gcccggaccc
    18241 gccgcaacac ccgcaacggc cgacatgcca gcgaacacgt cgtcgcggcc gcccgcgcga
    18301 tcttcaaccg cgccatcgcc gacgacccat ccccgccgcg gcaagccccg ctcaccgcgt
    18361 cgccaaaccc cgcaggccgg cggtgcctcg caggccaggt ccggcatggg caggaccggc
    18421 atgttcggcg agcatgcccg ccccgaggtc acggcggtca ctcagtctgc ccgcgagcag
    18481 aaccagaacg gcggaaagac caggccttcg tgtggccgaa gttcttgcgc agaccacgga
    18541 tctcgatcgc gttcaccggg caccgtccag caggacgcgg cgttcggcgg ccgcgatcgc
    18601 gcgcagggac acgtcgacga cgtcggagtt cacggagatg gacgtgatcc cgaaccgcac
    18661 gagatgctcc gcgaaaccgg gcttcaccga aggcgcctgc ccgcacaacg aggaggtgat
    18721 ccccgcgtca cgggcagcac cgatgatacg tccgatggtg tcgagcacgg cggggtcggc
    18781 ttcgtcgaac agctccgcgc agaccgccga atcccggtcc accccgagaa ccagctgggt
    18841 gagatcgttg ctcccgatgg acacgccgtc gatgcccaat ccggcgtact cgggaagcca
    18901 gtgcaccacg gacgggacct cggccatgat ccagcggtgc agtccacgct gccctccgag
    18961 cgggctcgcg tcgatcaggt ccagacaggc ctccagttcc catttggtac ggacgaatgg
    19021 gatcatcagg tgaaggttcg gcgtctgttc gcgtactcgg gcgagggtgc ggagttcgag
    19081 gtcgaacagg tccttttccc gcacgtagcg gaagcaaccg cggtagccga tcatcggatt
    19141 gctctccgcg gcttcgtagg cctcgccccc ggcgagaccc cggaactcgt tgctgcgcag
    19201 gtcggttgcc cggtagatga ccggtcgcgc gccgaaggga cgggtgatcc gcaacagcga
    19261 ctcggtcatg gagtccacaa aggactcctc ttcgcccctg gcgatgaggt cgcggggatg
    19321 acgtccgccg agcgcttcgg tcagcaggaa ctccgctcgc agcaacccga ccccgtcgac
    19381 ctgacgcgcg gcgacttcct cggcgtgttc aggcatggct agattgacgt acagtctggt
    19441 cccgatcgtc tccggcgcgg caggcgccgc gatcggttcg gtgcgcgccg gcggctggac
    19501 gtagccggac cgcacctcac cgctggttcc gtccacggtg acgggcccgc gtcccgcgag
    19561 cacccgtgtc gcgtcgccgg tgccgacgac gcagggcacc cgcagctccc gcgcgacgac
    19621 ggcggcatgg caggtcatcc cgccgccgtc ggtgaccagc gccgaggccc gccggatggc
    19681 gggaacccag tcggggttgg tcatcggcgc gaccagcact tcgccatccc tcagctgccc
    19741 gccttcgccg ggatcacgca gcactcgtgc gttcccgtca ccgacgcccg gtgacgctgc
    19801 cagtccggtg acgagaacgt ccggcggctg ctcacgatcc ggcggcagcg tggtgaccgg
    19861 ccgggactgg acgacccaga tctccttgcc cgccatagcg aactcgatgt cctgtggcgt
    19921 cccgtagtgg tcttcgatcc tggtggcgag ccgtgccagc tcaaggacgg tcgcgtcatc
    19981 gagcacccgg cgtcccgccg tctggctagg aagggcgacc ttgacgtctc ccccgcccac
    20041 ttcggcgatg atcttgtggg tcttgctgcc cacccgtgcc gaaaccagcc gcagatcttc
    20101 cttggcgacc aggtaggtgt ccggttcgac cgctccggag acgatcgctt cgccttggcc
    20161 gaagaccgct tcgacgacca ggcggtcacg atcgccggac cgcggatccg cggtgaagat
    20221 gaccccggag cgatccgcgg cgatcatcct ctgcaccacc accgcgatcg cgggctcccc
    20281 ggtgaaactg c
//
