LOCUS       NZ_KB913032            10390 bp    DNA     linear   CON 28-APR-2024
DEFINITION  Amycolatopsis alba DSM 44262 scaffold1, whole genome shotgun
            sequence.
ACCESSION   NZ_KB913032
VERSION     NZ_KB913032.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Amycolatopsis alba DSM 44262
  ORGANISM  Amycolatopsis alba DSM 44262
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1  (bases 1 to 9811274)
  AUTHORS   Klenk,H.-P., Huntemann,M., Han,J., Chen,A., Kyrpides,N.,
            Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N.,
            Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N.,
            Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Amycolatopsis alba DSM 44262
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: India
            num_replicons                     :: Missing
            ref_biomaterial                   :: DSM 44262
            biotic_relationship               :: Free living
            rel_to_oxygen                     :: Aerobe
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.05; ALLPATHS v. r41043; Phrap v. 4.24
            finishing_strategy                :: Missing
            environment                       :: Soil
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi10729
            Current Finishing Status          :: Level 2: High-Quality Draft
            Type Strain                       :: Yes
            Funding Program                   :: DOE-CSP 2011
            Isolation Site                    :: Soil
            Cell Shape                        :: Filament-shaped
            Motility                          :: Nonmotile
            Sporulation                       :: Sporulating
            Temperature Range                 :: Mesophile
            Gram Staining                     :: Gram+
            Diseases                          :: None
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.05; ALLPATHS v. r41043; Phrap v.
            4.24
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina GAii
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000384215.1-RS_2024_04_28
            Annotation Date                   :: 04/28/2024 01:36:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,988
            CDSs (total)                      :: 8,922
            Genes (coding)                    :: 8,855
            CDSs (with protein)               :: 8,855
            Genes (RNA)                       :: 66
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 24 of 67
            Pseudo Genes (incomplete)         :: 49 of 67
            Pseudo Genes (internal stop)      :: 4 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:42:18
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2966831
            Orig. end                         :: 2977221
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB913032.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 404842
            Source DNA and Organism available from Hans-Peter  Klenk
            (hpk@dsmz.de)
            Source DNA available from Hans-Peter  Klenk (hpk@dsmz.de)
            Organism available from Hans-Peter  Klenk (hpk@dsmz.de)
            Contacts: Hans-Peter  Klenk (hpk@dsmz.de)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10390
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="12"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10390
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10390
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5390](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5390)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(5..20)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.77"
                     /tool="antismash"
     gene            complement(572..3361)
                     /locus_tag="AMYAL_RS0113950"
     CDS             complement(572..3361)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631926.1"
                     /locus_tag="AMYAL_RS0113950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_020631926.1"
                     /transl_table=11
                     /translation="MGESGSPARFQLLGPVRLLDGERPVPVGGPGVRGLLALLALKVNK
                     VVALDEIIDALWGHDPPATARTIVHGNVSHLRRVLRDIQGDHPGGARILTAPPGYQLTV
                     EPGRIDVHRARSLLEQSSAEDPDKASELLAEALALWQGPALAGVPGSVRAPELEDLRLA
                     VHGARVDADLELGRHAELIVELSPLVRASPLAERTAGQLMRALYHAGRRGDALELYRTV
                     SRATLRTLGVEPGADLRWLHERVLNDDLPVKAFADPKAETAPVQQLPPAVPSLAGREAD
                     LAWLDDLAERAEAGETAVGVVSGTAGIGKSSLVVWWAHRAVRRFPDGILFASLRGFDPH
                     HPPLEPADLLTQFLLGLGVPSDGVPEQVHERIALYRSMIAGRRMLVILDNARSAEQVRP
                     LLPPGSRSMTLVTSRSRLDGLAVSNAAKLRVLGTLAPGDAVRLIEELAGPAGFDLNHAL
                     ARLCGYLPLALRIAGARLAASAQWSAQDLVDELGNERTRLAALDVEGPDDGVRAAFDVS
                     FRGLPSEVADTFLRLGVVQVVSAGSYLTAAIGEITVAEARRHLRVLTAHNLLTETGRDS
                     FAPHDLVRLFLRELAENELDETDREDVLSRSVRFYQAVADRARRKMLRIVDPLDFTGVL
                     TDAQTPPLGSFAEAQDWFTAEWENLVEVLEAARAGGRHDDVWRLARVAHTYRAVYPLLD
                     EWTRLVEIGLEAAERSGDVLGRCWMLISRCAIALTFELPQGCLADAESALELASAIGDN
                     RLMVSANIHLGSALTLLERYDEAIATLRQAAEETERTGDLELRGQALNNCAEAEKRSGR
                     FIEAIGHQVASLEIDRTLGDDSYVVVSLNNLAELSMRIGELAAAERYVWEAVDLTISRR
                     FVLQEGVLRLTLGRVLRTGSDVDGAREQFALALKILADANPKLAALVRAELTDLGEGP"
     gene            complement(4077..4976)
                     /gene="thpD"
                     /locus_tag="AMYAL_RS0113955"
     CDS             complement(4077..4976)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="AMYAL_RS0113955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_020631927.1"
                     /sec_met_domain="PhyH (E-value: 8.3e-32, bitscore: 110.2,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTDTRVDDSYPTRITGKPEQLPRTHPTVWGTEADGPIDAAALA
                     NHETRGYTVVDQLLSPGEIQTYWQDLVRMSSEDHRDDERVITEAKTGEVRSIFDVHETS
                     ELIAELVRDPRVLDRAQQILGSEVYIHQSRVNYMPGFKGTGFYWHSDFETWHAEDGMPA
                     PRAVSCSIALTDNYPFNGGLMVMPGSQRTFVQCAGETPEDNYKSSLKEQRAGVPSEEDI
                     TKLAAEYGIDQFTGQAGSALWFDSNVMHGSSNNITPYPRSNIFIVFNSVENALRNPYAA
                     IEPRPAFIAGRDSSPLTR"
     gene            complement(5001..5390)
                     /locus_tag="AMYAL_RS0113960"
     CDS             complement(5001..5390)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165143.1"
                     /locus_tag="AMYAL_RS0113960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_007033454.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.2e-53, bitscore:
                     179.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVRTLDEVTDTDADIKTPNWRSKRIVLAKEGVGFSVHETTLYAG
                     TVNDFWYANHIEAVFITSGEGELENTATGEVFQLKPGTLYLLNDHDKHQVRPKTEIKCV
                     CVFNPPITGREVHDENGVYPLVTEP"
     gene            complement(5434..6684)
                     /gene="ectB"
                     /locus_tag="AMYAL_RS0113965"
     CDS             complement(5434..6684)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 2.8e-73.
                     Score: 238.8. Matches aSDomain:
                     nrpspksdomains_AMYAL_RS0113965_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033455.1"
                     /locus_tag="AMYAL_RS0113965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_020631928.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.9e-74, bitscore:
                     249.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFEELESEVRSYSRGWPVVFDRAQGSYLYDEDGKAYLDFFAGA
                     GALNYGHNNPALKRALIDYIARDGVTHALDMFTVAKRDFLQTFKEKILQPRDLDYKVVF
                     PGPGGANAVEAALKLARKVTGKESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHA
                     TPMPYDNYFDGSIPDFLYFEKLLEDSGSGLNEPAAVIVEGVQGEGGINAARIEWLKALD
                     DLCKKHGILLILDDVQMGCGRTGPFFSFEDAGIKPDIVCLSKSIGGYGIPMALTLIRPD
                     LDVWEPGEHNGTFRGISPAFVTATEALRVYWSDDELEKSTKAKGERIAAAFQGIVEAYP
                     DANLFAKGRGLARGIEFANGDLAGKVCAAAFERGLLMETSGPDGEVMKVLPPLTLTEDE
                     LTKGLSIIDEAVATVLS"
     aSDomain        complement(5611..6621)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AMYAL_RS0113965_Aminotran_3.1"
                     /evalue="2.80E-73"
                     /label="AMYAL_RS0113965_Aminotran_3.1"
                     /locus_tag="AMYAL_RS0113965"
                     /protein_end="358"
                     /protein_start="21"
                     /score="238.8"
                     /tool="antismash"
                     /translation="FDRAQGSYLYDEDGKAYLDFFAGAGALNYGHNNPALKRALIDYIA
                     RDGVTHALDMFTVAKRDFLQTFKEKILQPRDLDYKVVFPGPGGANAVEAALKLARKVTG
                     KESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHATPMPYDNYFDGSIPDFLYFEK
                     LLEDSGSGLNEPAAVIVEGVQGEGGINAARIEWLKALDDLCKKHGILLILDDVQMGCGR
                     TGPFFSFEDAGIKPDIVCLSKSIGGYGIPMALTLIRPDLDVWEPGEHNGTFRGISPAFV
                     TATEALRVYWSDDELEKSTKAKGERIAAAFQGIVEAYPDANLFAKGRGLARGIEFA"
     gene            complement(6723..7214)
                     /gene="ectA"
                     /locus_tag="AMYAL_RS0113970"
     CDS             complement(6723..7214)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664377.1"
                     /locus_tag="AMYAL_RS0113970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_026467053.1"
                     /transl_table=11
                     /translation="MSGKHLIENPTKADGAALWRIARDSKKLDLNTPYAYMLWCHDFAD
                     TSVVAKVDGEPVGFVIGYRKQDTGFVWQVAVDASQRGKGLAGALLDELFDQLVAQGVRY
                     LETTITPDNEASIRLFASFAKRWDATLERQDLFSATDFPAEEGTHEQEDLYRIGPLGNN
                     "
     misc_feature    complement(7998..8009)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     misc_feature    complement(9932..9952)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.8"
                     /tool="antismash"
ORIGIN
        1 ggttactggt ctacaccaac ttcttcgcca ccggtcggtc cggactgcac atcccggcgg
       61 ccgcgggcga caagaacacc gtcgagttcg tcgtcagcgc ctccggtggc gcgaccgtcc
      121 ggttcggttc gctcaacgac cagcggagcg agacgacccc ggccagcacc gacgaatggc
      181 gtggcacggc gtccttcaac tcgggcgact acctgctcac cgtcaccgcg gacacccgga
      241 acgcgtcggt cagcaaccag atctcctgca cgatccgggt caacggccag aaggtggcgg
      301 agaacaaggg ccagaccatc gccctgtgca ccgcgagcac cggctgatcc ccgcgcgttt
      361 cggattaccc gcctggcggc cggccggtat cgcccccacc tccaccggcc ggaccgccac
      421 gagccgctcc tggcggctct tcaggcgaac cccgaaaggc ccgcccgagc gccgatctct
      481 accgaatgcc cctcccctag ggctcgatcg acgctgaacg ggaccctacg cgcgtgagac
      541 acaaggccac taagaatccg ctaaggatca ctcagggacc ttcgccgagg tcggtgagtt
      601 ccgcacggac cagggccgcg agcttcggat tcgcgtccgc caggattttc aacgcgagcg
      661 cgaactgttc gcgggcaccg tccacatccg acccggtccg cagcaccctg cccagggtca
      721 gccgcagcac gccttcctgg aggacgaacc gacggctgat ggtcaggtcg acggcctccc
      781 agacgtaccg ttcggcggcg gcgagttcgc cgatccgcat gctcagttcg gccaggttgt
      841 tcagcgagac gacgacatag ctgtcgtcgc cgagggtccg gtcgatctcc agcgaggcga
      901 cctgatgccc gatcgcctcg atgaaccgcc cggaccgttt ctcggcctcc gcgcagttgt
      961 tgagcgcctg cccgcgcagt tcgaggtcgc cggtccgctc ggtctcctcg gccgcctggc
     1021 gcaacgtcgc gatggcttcg tcgtaccgct ccagcagggt cagcgcggag ccgaggtgga
     1081 tgttcgccga gaccatcagc cggttgtcgc cgatcgcgct cgcgagttcc agcgcgcttt
     1141 cggcgtcggc gagacagccc tgcggcagtt cgaaggtgag cgcgatggcg caccgcgaga
     1201 tcagcatcca gcagcggccg aggacgtcac cggaccgctc ggctgcctcc agtccgatct
     1261 cgaccagccg cgtccactcg tccagcagcg ggtacaccgc gcgataggtg tgcgcgaccc
     1321 tcgccagccg ccagacgtcg tcgtggcgcc cgccggcgcg ggcggcttcg aggacctcga
     1381 cgaggttctc ccattccgcg gtgaaccagt cctgtgcctc ggcgaaggag cccagcggcg
     1441 gcgtctgcgc gtcggtcagc acaccggtga agtcgagcgg gtcgacgatc cgcagcatct
     1501 tccgccgggc cctgtcggcg acggcctggt agaaccgcac cgaacgcgac aggacgtctt
     1561 cccggtcggt ttcgtcgagt tcgttctccg ccagttcgcg caggaacagg cggacgaggt
     1621 cgtgcggggc gaaggagtcg cggccggttt cggtgagcag gttgtgcgcc gtgagcaccc
     1681 gcagatgccg ccgcgcctcg gcgacggtga tctcgccgat cgccgcggtc agatacgagc
     1741 ccgcggagac gacctggacc acgccgagcc gcaggaacgt gtccgcgacc tcggacggca
     1801 gcccgcggaa cgacacgtcg aacgccgcgc ggaccccgtc gtccgggcct tcgacatcga
     1861 gcgcggccag cctggtgcgt tcgttgccga gctcgtcgac gaggtcctgc gcggaccact
     1921 gcgcgctggc ggcgagccgg gcgcccgcga tccgcagcgc cagcgggaga taaccgcaga
     1981 gccgggcgag cgcgtggttg aggtcgaatc cggcgggccc ggcgagttcc tcgatcagcc
     2041 ggacggcgtc gccgggcgcg agcgtgccga ggacgcgcag cttcgccgcg ttggacacgg
     2101 cgagcccgtc gagccgggat cggctggtca ccagcgtcat cgaccgcgaa ccgggcggca
     2161 gcaacggccg cacctgctcg gcggaccggg cgttgtcgag gatcaccagc atccggcgcc
     2221 cggcgatcat cgagcggtag agcgcgatgc gttcgtggac ctgctccggg acgccgtcgg
     2281 aggggacgcc gagcccgagg aggaactggg tgagcagatc ggcgggctcc agcggcgggt
     2341 gatgcgggtc gaaaccccgc agcgaggcga aaaggatgcc gtcgggaaag cggcggacgg
     2401 cgcgatgcgc ccaccacacc accaagctgc ttttgccgat tccggcggtc ccggagacca
     2461 cgccgaccgc ggtctcgccc gcctcggccc gctcggcgag gtcgtcgagc caggccagat
     2521 cggcctcacg tccggcgaga ctcggcacgg cgggcggcaa ctgctgcacg ggcgccgttt
     2581 cggccttcgg atccgcgaag gccttgaccg gcagatcgtc gttcaggacg cgttcgtgca
     2641 gccagcgcag gtccgcgccg ggttcgacgc cgagcgtccg cagggtcgcc cgcgacaccg
     2701 tgcggtacag ctcgagcgcg tcgccgcggc gaccggcgtg atagagcgcg cgcatcagct
     2761 gaccggccgt gcgctcggcg agcgggctcg cccggaccag cgggctgagc tcgacgatca
     2821 gttccgcgtg cctgccgagt tcgagatccg cgtcgaccct ggcaccgtgc acggcgagcc
     2881 gcagatcttc cagctcgggc gcgcggaccg aacccggcac accggccagc gcgggcccct
     2941 gccacagcgc gagtgcctcg gcgagcagct cggacgcctt gtccggatct tccgccgagg
     3001 actgttccag cagcgaacgg gcacggtgga catcgatccg gcccggctcg acggtgagct
     3061 gataaccggg cggcgcggtg aggatccggg cgccgcccgg atggtcaccc tggatgtcac
     3121 ggagcacgcg acgcagatgg gaaacgttgc cgtgcacgat cgtgcgggcg gtggcgggcg
     3181 gatcgtggcc ccagagcgcg tcgatgatct cgtcgagcgc gacgaccttg ttgaccttca
     3241 acgccagcag cgcgagcaga ccgcggacac cggggccgcc gaccgggacc ggccgttcgc
     3301 cgtcgaggag gcgcacggga ccgaggagct ggaagcgagc aggggaaccg ctttcgccca
     3361 tggcaagccc acctcctccg atctctcccc ttaccggagt agccaaccta ccgcgcgaag
     3421 aggccgccaa ccaggaaaca cgaggaaaga gagacatagc tggtcggaag cggtcaacgg
     3481 gaaatgacca aaatcgatgt gagtggtaag tcggttccga tgagggaaag tcaacgtgtc
     3541 caggcgggtg gcgggtgggc gtgtcgggga aggctccctt cgcgtcacgc ccggccgcgc
     3601 ttcggatgcc ccaaagggcc ccttcaggac gttgaacgtc ctgaaggcgc ccttcaggac
     3661 acgacgtcga gtcacgagac ctcagcctcc aggcacgtcg cgagcgaaag tgcgggaccg
     3721 gcggactgtc tccccggaag cgcgccggcg cgacggggag acttcacccc gcgcgcctct
     3781 tcgtcagcgc gcggcagcag aacagtgcgg caataaaaaa ggctccgtgc catcagtccc
     3841 gatctgatgg cacggagccg ggggatgtcc gatttgggcc gcctgggggc gcgacccaca
     3901 ccggacatcg gcgtccacgt cgtccgggtg ggctcacccc gagcgaagaa cccgttcgac
     3961 cgtcgacaac acctacgtga gcgagcccac tggcccgctc acgtggcgac aacgtaacag
     4021 ggcgcacacc gacggctaca gccgccccgc catggcctgc gctacctgcg gtttcttcag
     4081 cgcgtcagcg gggacgagtc gcggcccgcg atgaacgccg gacgcggttc gatcgcggcg
     4141 tacgggttcc gcagcgcgtt ctcgacgctg ttgaacacga tgaagatgtt cgacctcggg
     4201 tacggcgtga tgttgttcga cgaaccgtgc atcacattcg aatcgaacca gagcgccgaa
     4261 ccggcctgtc cggtgaattg atcgattccg tattcggcgg cgagtttggt gatgtcttcc
     4321 tcgctgggaa cgcccgcccg ctgctctttc agcgaactct tgtagttgtc ctccggggtc
     4381 tccccagcgc attggacgaa ggtccgctgc gaacccggca tcaccatcag cccgccgttg
     4441 aacgggtagt tgtcggtgag cgcgatggag cagctgaccg cccgcggcgc gggcatgccg
     4501 tcctcggcgt gccaggtctc gaagtccgag tgccagtaga acccggtgcc cttgaaaccg
     4561 ggcatgtagt tcacccggct ctggtggatg tagacctcgg agccgaggat ctgctgcgcc
     4621 cggtcgagca cgcggggatc gcggacgagt tccgcgatca gctcactggt ctcgtggacg
     4681 tcgaagatcg agcggacctc accggtcttg gcctcggtga tgacccgctc gtcgtcgcga
     4741 tggtcttccg acgacatccg gacgagatcc tgccagtagg tctggatctc gccaggcgac
     4801 aacaactggt cgacgacggt gtaaccacgg gtctcgtggt tggcgagcgc ggccgcgtcg
     4861 atgggaccgt cggcctcggt gccccagacg gtggggtgcg tcctgggcag ctgttcgggc
     4921 ttacccgtga tgcgggtcgg gtaactgtcg tcgactcggg tgtcggtcag cgtcaaagcg
     4981 gtcgcctcct cgagcgattc ctagggttcg gtgacgagcg ggtacacgcc gttttcgtcg
     5041 tggacttcac gaccggtgat gggcgggttg aacacgcaga cgcacttgat ctcggtcttg
     5101 ggccggacct gatgcttgtc gtggtcgttg agcaggtaca acgtgcccgg cttcagctgg
     5161 aagacctcgc cggtcgcggt gttctcgagc tcaccttcac cggaggtgat gaagaccgct
     5221 tcgatgtggt tcgcgtacca gaaatcgttg accgtcccgg cgtagagcgt ggtctcgtgc
     5281 accgagaaac cgacaccctc cttggccagc acgatgcgct tgctgcgcca gttcggggtc
     5341 ttgatgtcgg cgtcggtgtc ggtgacctcg tcgagtgttc tgacaagcaa cggaaaactc
     5401 cttctcgttc ctggttgtgg cggtcggttc gggtcagctg aggacggtgg cgacggcctc
     5461 gtcgatgatc gacaggccct tcgtgagctc gtcctcggtc agggtgagcg gcgggagcac
     5521 cttcatgacc tcgccgtcgg ggccggaggt ctccatgagc agcccgcgct cgaacgcggc
     5581 ggcgcagacc ttgcccgcga ggtcgccgtt ggcgaactcg atgccgcggg cgaggccgcg
     5641 gcccttcgcg aacaggttcg cgtccgggta cgcctcgacg atgccctgga acgcggcggc
     5701 gatgcgctca cccttggcct tggtcgactt ctccagctcg tcgtcgctcc agtagacgcg
     5761 cagcgcctcg gtcgcggtga cgaacgccgg gctgatgccg cggaaggtgc cgttgtgctc
     5821 acccggctcc cagacgtcga ggtccgggcg gatcagcgtc agcgccatcg ggatgccgta
     5881 gccgccgatg gacttcgaca ggcagacgat gtcgggcttg atgcccgcgt cctcgaagct
     5941 gaagaacggg ccggtgcggc cgcagcccat ctggacgtcg tcgaggatca gcaggatgcc
     6001 gtgcttcttg cacaggtcgt ccagcgcctt gagccactcg atgcgcgcgg cgttgatgcc
     6061 gccctcgccc tggacgcctt cgacgatgac ggcggcgggc tcgttcaggc cgctgccgga
     6121 gtcttcgagc agcttctcga agtagaggaa gtccgggatg gagccgtcga agtagttgtc
     6181 gtacggcatc ggggtggcgt gcaccagcgg gacacccgcg ccgccgcgct tcatcgagtt
     6241 gccggtcacc gaaagggcgc ccagcgtcat cccgtggaag gcgttggtga agttgatgac
     6301 cgattccttg ccggtcacct tgcgcgcgag cttcagcgcg gcctcgaccg cgttcgcgcc
     6361 accggggccg gggaagacga ccttgtagtc gagatcgcgc ggctggagga tcttctcctt
     6421 gaacgtctgg aggaagtccc gcttggccac ggtgaacatg tcgagcgcgt gggtgacgcc
     6481 gtcacgggcg atgtagtcga tcagggcccg cttgagcgcc gggttgttgt gcccgtagtt
     6541 aagcgcgccg gcgccggcga agaagtcgag ataggccttg ccgtcttcgt cgtacaggta
     6601 gcttccctgc gccctgtcga acaccacggg ccagccacgg ctgtaactgc gtacttcgga
     6661 ttcgagttcc tcgaaaatac tcatcacgtt ttctttctcc ctgggatccc cggttttacg
     6721 tcctagttgt tgccgagcgg accgattcgg tacaggtcct cctgctcatg cgttccttcc
     6781 tcggccggga aatccgtcgc ggagaacaga tcctggcgtt ccaaggtggc gtcccatcgc
     6841 ttggcgaacg aggcgaacaa ccggatcgaa gcctcgttgt ccggagtgat cgtggtttcg
     6901 aggtaccgga ccccctgggc gacgagctgg tcgaacagct cgtcgagcag tgccccggcc
     6961 aggccttttc cgcgctggga cgcgtcgacc gcgacctgcc agacgaagcc ggtgtcctgc
     7021 ttccgatacc cgatcacgaa gccgaccggc tcgccgtcga ctttcgcgac gaccgaggtg
     7081 tcggcgaaat cgtggcacca cagcatatag gcgtaaggcg tattgagatc gagcttcttc
     7141 gaatcgcggg cgattctcca gagcgcggca ccgtctgcct tggtcgggtt ttcgatcaag
     7201 tgctttccgg acatgtccaa gaaacgtacc ggaggattcg ggcgcggtca gatcagcgcg
     7261 gcggcggcaa cgcggctacc tgcaataacc tcgaaacaca cgtgacaaag gtaacggtaa
     7321 cgtttgaagc gcagcgctcg tgggtaaggc cgcttggaga cggtctcact ggatgtgacg
     7381 ataccaaccg ttaccacttc gtcgcacggt caggacgcga ttcgggggcc cgatccgcga
     7441 ggaggggcct acttgcggtg agctcgcctc cggaagacgg aacccagcgg cgtccagatg
     7501 gcgggcagca ggaacagcag gcagacgacc agccaccaga ccggtgcctc gccgcgccgc
     7561 cagcccgcga cgacgccgcc gagccagccg aacagcgggt cccggatcgg cggcaggaac
     7621 gcggcgaaga gcagagccgc ccccaccagc acgccgagca ccgtgagcac catccgccac
     7681 gtcttgcgca acgtcatcag gctgaccacc aggaacacga tggcgcccgc ggcgaccggg
     7741 acaccgaccc actgcaggac cgggccgccc tgcccgccga tgacgaaacc cgccagcacg
     7801 gtgatcagcg ccacgagcac gttccacggt gacggcaggg tggccgcgcc gcgcgtgatc
     7861 caccaggcgc tgcgcccggc ggcctgggcg aaggtcgcgg cgggctgcac cgatttcggc
     7921 atcctggtcg cccccgcggc cgcgttgaga ccggcagcac ccgccttgac cagtgtcttc
     7981 gtgagcagca tcgtcccgcg ttcgtgttcg aaccgttcgc cggagacctg gcacgcctgg
     8041 aacgcgcgct gggtctgctc ggcggtttcc ggctcgctct cgatcagctg ccggaaccgt
     8101 tccgacggtt tcccgttgcc cttggccgtt ttcgcgtctt gtccgcaatg gaggccgacg
     8161 cagggcagtt cctcgcgcgc gatctccagc tgccggaccc gcgcgagcac catcgccgtc
     8221 accggcatgc tgatcgggag ggcggcttcg ccttccacgt cggcgagttc gccgtcgagg
     8281 ccgaggaaag ccagttcctc cttcagctgc gcgcacaggg cgtccgcttc ctcttgggag
     8341 agcccgtctt ccgtaccggg acggcgccag ccgatcttct cgaaggtgtc ccgcacctgc
     8401 gtccggaacg cctcgcgccc gacgacgtcg cgcagcagcc gcaggcgttt cgggtcgagc
     8461 aggcactgga ccagccagcc cgcgccgtcg acccggcccc acatccagtc gttcgcacgc
     8521 caggacgcct tgtagaacgc gccgaagtag tcggcctgca taccggtgag cttgtcccac
     8581 gaacggcggc gcttcatgtc cagcagcgtc cgcgaatccg cgctcacctg gacgaggtcg
     8641 acccgctggt cgaccgacgg cggctgcgcg agcagacccc gcgtcgcgat gtgcagccgc
     8701 accagccggg aggtgacgac cgcgtcgccg gcgcttttct cgtcggaggt gagcgccgtc
     8761 cacgccaaca gcgtgctcac cgtgtcggcg gcttcggttt cgggtttccc ttgcgccagg
     8821 ggcaaaagta tcggcgtcgc ctgccggagc gcggcgacca cgagcggcca cgcctccttc
     8881 agcgtgtccg accggcccat ctcggcccat ttcctggcga gcccttcgat ccaggccgcc
     8941 agcgtgtcct cggacttcgg cggatcctgc cgggtgaccc agcccgcgat ccggtagccc
     9001 ctggcggcct tgtgccgggc ctcgtgcagc agcacacggc aacgattgag ctgcgtcgcc
     9061 tgctccggcg acggatcgag ccggaagccc gcgttcacca gctgcaggcc ggtcgcgacc
     9121 gaatcgtcga gggcggtggt gcggaaggcg atcaggtcgc cgagttcgca ggtgtccttc
     9181 ggcggctcgg tgggcaggcc gtcgcgcagg cccgacgccg cggcagcgcg cagctgtgcc
     9241 gccgtgccgg acgcccactg acggcctgga tcctccacat cggacagtga gagcctgcgg
     9301 gtcgcctcac ggaccagcgc ggtggcgtcc tcctggaccc gccgatcgcg gtagtcgttc
     9361 atcagccgct ggtcgaccag cgtgtccgga ccgccgcgaa cccccatcgc ggcaagggaa
     9421 acacgggttc ccctggtccg gacgacggcg tcgttgtgcc gggtcaggtc ttcgagttcg
     9481 gcgctgatcg tctggctcag cacggtgccg acgaccttgc tcatcgcgct gcccagcagc
     9541 ggcggccgct ccggatcgac cacgacccgt tcggcttcgc gttccgccgt cggcaccacg
     9601 tacagcagca gccgccggac gtccgaatgc gactgccgtt cgaagatctc ccgcagcgcc
     9661 gggcgcaacg gcttgttcag cagcacaccg ccgtcggtga gccaatgcga ccgcgagacg
     9721 tcggtgtact cggtcatgtc cggatgcaac gggccggtct tctcgccgat cggcatccgg
     9781 gaaagttcga acgcgccggg gaacgacgcg gtggagcgcg ccgcgagcgc gagcgggccc
     9841 accacgtcat ctgtccacaa aggaccatcg aagcggaaga gcaggcgatg ttcggtgtcc
     9901 cggaccaggt tgccgagcgc gtcgtcgaac ctggtcgtct cgccgtcgat catcgtgccg
     9961 gtgagcagca cggtgatgtc ggggcagtcg ggcttcaccg tcgccttgtc ggtgatccgg
    10021 tgcagcgcgt ccttcagatc cgccagcaga accttgtcgc cgtcgagcag cgagcgtggt
    10081 gccttttcgc cggggtcacg gatcaggttg tcgagcgatc ccgtgctgat ccacgtgtcg
    10141 cgcagcacct gcgggctcga ccggaacgcc tccgccagcc cgaggcacgc cgcgttgatg
    10201 ccgcccgcgc tcgtgccggt gagcacgtcg agtgagacgg tgacgttgag caggtcgagc
    10261 agggcacggt acggcccttc ggcttccggc gttctcgacg cctggagcag atgggaggtc
    10321 tcggtggcga ccccgcccat ccagaccgcg aggctcgccc cgcccaccat cgtcatcgcc
    10381 agccggatct
//
