LOCUS       NZ_KB905836             9160 bp    DNA     linear   CON 02-MAY-2024
DEFINITION  Streptomyces sulphureus DSM 40104 F603DRAFT_scaffold00024.24, whole
            genome shotgun sequence.
ACCESSION   NZ_KB905836
VERSION     NZ_KB905836.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sulphureus DSM 40104
  ORGANISM  Streptomyces sulphureus DSM 40104
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K.,
            Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A.,
            Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name :: Streptomyces sulphureus DSM 40104
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: DSM 40104, ATCC 27468
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. R37654
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi11971
            Current Finishing Status          :: Level 1: Standard Draft
            Type Strain                       :: Yes
            Funding Program                   :: DOE-CSP 2012
            Sporulation                       :: Sporulating
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. R37654
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000381025.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 01:54:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,193
            CDSs (total)                      :: 6,120
            Genes (coding)                    :: 6,022
            CDSs (with protein)               :: 6,022
            Genes (RNA)                       :: 73
            rRNAs                             :: 6, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 37 of 98
            Pseudo Genes (incomplete)         :: 75 of 98
            Pseudo Genes (internal stop)      :: 11 of 98
            Pseudo Genes (multiple problems)  :: 20 of 98
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:55:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9160
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB905836.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405920
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Nikos  Kyrpides (nckyrpides@lbl.gov)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-PGF and JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     cand_cluster    1..9160
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /detection_rules="botH"
                     /kind="interleaved"
                     /product="RiPP-like"
                     /product="bottromycin"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     gene            <1..291
                     /locus_tag="F603_RS34890"
                     /pseudo=""
     CDS             <1..291
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bottromycin_Methyltransferase_RRE"
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000049: Methylation (68% identity)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019887074.1"
                     /locus_tag="F603_RS34890"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="RiPP maturation radical SAM protein 1"
                     /pseudo=""
                     /sec_met_domain="Bottromycin_Methyltransferase_RRE
                     (E-value: 2.6e-30, bitscore: 93.8, seeds: 12, tool:
                     rule-based-clusters)"
                     /transl_table=11
                     /translation="HETVLQGDDAALFRAVIGGTRIRDLQANGWPEGQPWDRAWEKLQN
                     WQRRRWIFIEGTKIIALAIREQPSAYRTPPPKGTPRRQARNPVPVTLTSRK"
     source          1..9160
                     /culture_collection="DSM:40104"
                     /db_xref="taxon:1123321"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sulphureus DSM 40104"
                     /strain="DSM 40104"
                     /submitter_seqid="F603DRAFT_scaffold00024.24"
                     /type_material="type strain of Streptomyces sulphureus"
     region          1..9160
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /product="bottromycin"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /rules="botH"
                     /tool="antismash"
     protocluster    1..9160
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[579:4917](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      580..4917
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     protocluster    1..9160
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[4903:5746](+)"
                     /cutoff="20000"
                     /detection_rule="botH"
                     /neighbourhood="10000"
                     /product="bottromycin"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      4904..5746
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="botH"
                     /neighbourhood="10000"
                     /product="bottromycin"
                     /protocluster_number="2"
     aSDomain        4..174
                     /aSDomain="Bottromycin_Methyltransferase_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing methyltransferase protein in a
                     bottromycin cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_F603_RS34890_Bottromycin_Methyltransf
                     erase_RRE.1"
                     /evalue="8.00E-30"
                     /identifier="RREFam008.1"
                     /label="F603_RS34890"
                     /locus_tag="F603_RS34890"
                     /protein_end="57"
                     /protein_start="0"
                     /score="93.8"
                     /tool="antismash"
                     /translation="HETVLQGDDAALFRAVIGGTRIRDLQANGWPEGQPWDRAWEKLQN
                     WQRRRWIFIEGT"
     gene            376..516
                     /gene="botA"
                     /locus_tag="F603_RS34895"
     CDS             376..516
                     /codon_start=1
                     /gene="botA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019887075.1"
                     /locus_tag="F603_RS34895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bottromycin family RiPP peptide"
                     /protein_id="WP_106431447.1"
                     /transl_table=11
                     /translation="MGPAVVFDCMTADFLNDDPNNAELSSLEMEELESWGAWSDDTDQS
                     V"
     gene            580..1755
                     /locus_tag="F603_RS0131225"
     CDS             580..1755
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019887077.1"
                     /locus_tag="F603_RS0131225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_019549855.1"
                     /sec_met_domain="YcaO (E-value: 1.3e-13, bitscore: 40.5,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADSSATDCVLREVAFRSYRAEHTVTVQCTVRPDSGPGQAEGYGT
                     ATTEAVARKKALSEAVERLLACTPFALIARRPTPRTDRSRGTDPWQRGLRSAPAGCQVR
                     NYRSLSGEQPRQVPLFWSSPWVAGEEMRSANLAPQAARLSSTVGWAVAPSPEAALQGAL
                     YELTELLNYGAFLYRCLAAPRERPAPGGGPEPHEEGRTQTVPISWATRTPTILAVTRSS
                     DRLMPATGIGSGTTASEAAERAFLELAQAETLWRANSTAVPAERYFMRRFEKWPLLRRC
                     AALDFDLGEHAGPFDDGPHPSPLGALESRAIEVWADPGAVDISGADLRRTRLCFAHVVS
                     DPQPLLGLVRAGIPVFDTGEVRKILVCPRRARSADLSPSGRVAHSGRPTRA"
     misc_feature    615..629
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.33"
                     /tool="antismash"
     gene            1673..3007
                     /locus_tag="F603_RS0131235"
     CDS             1673..3007
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000053: Cyclization (77% identity)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019887079.1"
                     /locus_tag="F603_RS0131235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_106431448.1"
                     /sec_met_domain="YcaO (E-value: 7.7e-36, bitscore: 113.6,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVHAEHDLLTSALLDGSLTQDVPPERETPIGRALSAASDWLSSE
                     QLTPDIWKYGLDSAPTYEVHLKDAAGTLCSRSSGKGLGHRSMASALFESAEHYHLDWRR
                     DPLADEAAFVPGREVAGQPTAAGSALLHRLGGILGDSPVLTREYRPVAEALGATGGAAP
                     PHRHPVFLRDGGYRNWPHPSDDGSFKPLWHYTTSAGYAAGTTLHEALVHAVNELIERDA
                     WSYQLARSYFGLPDESGRALRVIDHESLPAELRALVDDVEASSGSPILIVSIPCDTGVP
                     AYVVCDAESDEDVRLIGSGASPVQAYALQRGLLEYLQVCTMYQHGPVDAEREAAQIATA
                     LARYPRHLAAAGFDVRQLPHERQPFDSDGLPAAATPEELLRHLIDRLQGVGVDLHFRVL
                     TRPGTITVVDVVAPGLEMLDKARAGYPVLPTGRLGERLRPQRREP"
     gene            3004..4917
                     /locus_tag="F603_RS0131240"
     CDS             3004..4917
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bottromycin_Methyltransferase_RRE"
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000046: Methylation (78% identity)"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298718.1"
                     /locus_tag="F603_RS0131240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_019549858.1"
                     /sec_met_domain="TIGR03975 (E-value: 2.5e-137, bitscore:
                     447.9, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="Bottromycin_Methyltransferase_RRE
                     (E-value: 2.9e-36, bitscore: 112.9, seeds: 12, tool:
                     rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLVLVAPPWNSLYRPSIQIATLAALSTEEQGDPISAVYGYLDWF
                     EYAVGFLECDPEQFAAVYDTIGEELYGLGVGDWIFGGGLPGAGDADEASATAEYGAFLR
                     EQGVDEEIVADVLRLREAAPRFVADLAERLVDDGAECFGFTTSFSQLVPSLAIAVAIKE
                     RAPDRLVLLGGANCDRPMGQAVMHAYPFVDAVVEGEGEQAVNALTRAKSVDDLAAVPGI
                     VLRHEDGLRTALPSIQPVESAPVPKYDAYFERLESLPWGDLIAPHVALPFQMSRGCWWG
                     EKLQCTFCGENGSGMVYRSKPGSQLVDTLRTLTERHGAFNTYAVDTILAKNDNGLAALA
                     DAEVDITTFFEVKANLGPREVGELRAAGVTMVQPGIESLSSGALKLLRKGATSWHNLRF
                     LVLCQELGIDAQWNLLSAMPDESAEMLEEQLRLIPFITHLAPPSTVSKVRVDRYSPYFE
                     KPDEFGIELRGPEKKYRFIHRGEGVDRTALAYSFEHGQQAGKERDDDAAMLALRRRLGA
                     RVGLWKKLHAQSRFSFRFGPNVTVVHDYRPHVGTGRTVLRGADDLLFRSLVHGGRLMQA
                     VGRVGEQTGTPESRLHERVSEWAACGWVHLEGRRGLVLAVRDGMRDELGRAAALRGSDA
                     ES"
     aSDomain        4597..4830
                     /aSDomain="Bottromycin_Methyltransferase_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing methyltransferase protein in a
                     bottromycin cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_F603_RS0131240_Bottromycin_Methyltran
                     sferase_RRE.1"
                     /evalue="8.90E-36"
                     /identifier="RREFam008.1"
                     /label="F603_RS0131240"
                     /locus_tag="F603_RS0131240"
                     /protein_end="609"
                     /protein_start="531"
                     /score="112.9"
                     /tool="antismash"
                     /translation="SFRFGPNVTVVHDYRPHVGTGRTVLRGADDLLFRSLVHGGRLMQA
                     VGRVGEQTGTPESRLHERVSEWAACGWVHLEGR"
     gene            4904..5746
                     /locus_tag="F603_RS33305"
     CDS             4904..5746
                     /NRPS_PKS="Domain: Abhydrolase_1 (40-158). E-value:
                     9.4e-14. Score: 43.5. Matches aSDomain:
                     nrpspksdomains_F603_RS33305_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     bottromycin: botH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000051: Epimerization (68% identity)"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1262: haloalkane dehalogenase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486710.1"
                     /locus_tag="F603_RS33305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_019549859.1"
                     /sec_met_domain="botH (E-value: 6.3e-147, bitscore: 476.9,
                     seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 4.1e-14, bitscore: 42.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2e-12, bitscore:
                     37.5, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRSPDREQAERFEVASVDGTLLRGFSRAARGSSDARGEPETLVLL
                     HGVAMDHRVWSESSFLDALPDDVHVVAPDLRGRGESDPGHSAEGHAIDRYVEDVRAVLG
                     FFGRERYSLFGLYFGGRIALRVAEADRRVQRAFSFCAHGEAVEVPADAVEEEAKEIEGP
                     GGYAYLRDHFSAAGAPAWMVSACDRVDQDELAAATRGLLHGSHQRAERGAADQDLVLIT
                     AEGDADLGTFREGSARLGARLWLVDSRTRVRAAARLAEVGTRTAELLTAAPAAMRGDA"
     aSDomain        5024..5377
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F603_RS33305_Abhydrolase_1.1"
                     /evalue="9.40E-14"
                     /label="F603_RS33305_Abhydrolase_1.1"
                     /locus_tag="F603_RS33305"
                     /protein_end="158"
                     /protein_start="40"
                     /score="43.5"
                     /tool="antismash"
                     /translation="TLVLLHGVAMDHRVWSESSFLDALPDDVHVVAPDLRGRGESDPGH
                     SAEGHAIDRYVEDVRAVLGFFGRERYSLFGLYFGGRIALRVAEADRRVQRAFSFCAHGE
                     AVEVPADAVEEEAK"
     gene            5743..7134
                     /locus_tag="F603_RS0131250"
     CDS             5743..7134
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000050: Hydrolysis (66% identity)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019887083.1"
                     /locus_tag="F603_RS0131250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_019549860.1"
                     /transl_table=11
                     /translation="MTDDALWTHRRGLLPDGDPLVGALEELAGRSRSHVLRAVGMLDAE
                     TGEAAPPRDLEVVDGAVARWSPAPDAAYCEWFALPGFVDAHAHVSSVTDLVGMLAYGVT
                     GYRQMWGEPAHLYSAGVHRARHAVLPLPWTTAGVVDGPDSRIPHHVTVVDDARQVRRVV
                     EDVLAFGFDGIKVYDDLARPAFEALVREAERAGIPVVGHVPEAVPLDAAYPGMWSTEHL
                     YGIVPHIFRLPAADRWAVLADALQRHRDDGPAVEGAAGRFVCPTLTAWRARSGERRYTR
                     PSRTLLQSATPSRRPAWHAAARDALSLERAEAQRRSLLVDRLGGIAYDLSRRGARLLVG
                     TDCGNPFVLAGPSYHKELAELSRSGLDFATVLKAATVDAYAATGRDPARADLVLYRRSP
                     AHDVTRLARPDAVLVDGVLLDADDLDHLWGLRLTAAGLDAGTWRRGDVDPAAGDRQNKE
                     TADAG"
     gene            7124..8347
                     /locus_tag="F603_RS0131255"
     CDS             7124..8347
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000044: Decarboxylation, Oxidation (74% identity)"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486713.1"
                     /locus_tag="F603_RS0131255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="WP_019549861.1"
                     /sec_met_domain="p450 (E-value: 1e-11, bitscore: 33.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQADPSTGTTDPEAPPGVCPVTGRRADPVDFEFFNGPSAYREAAA
                     THADEGAFYSASGDGFWVLTTYEGICEAFRDEEQFTVGRVSAADGAEEDRWIPLTIEGR
                     QHTEWRRRLGAWFTPQRVRELTPSIEANARRRIEGFVDDGEVSFNEDFARPFVLENLMT
                     AVGWEFDRFDQLLRIDRAMIDSRYAPDPREAAFEEVGIPALEKLAREQVERRRAEPADD
                     LTTATFGWEVEGRPVTDDDRVSLLCVLFLAGVDSTVNHLANAVQHLVCHDADKRRFLAS
                     REVRPRAVEEFLRANSCMYPGRLAARDGAGGTARRGETVLLPLAVANYDPEVFHDPEQV
                     DFDRERNPHIAFGTGAHQCLGAALARAQIRTALDVWHELIPHYGFPPQPEPSEPRFLRN
                     AYDLRLVW"
     gene            8355..>9160
                     /locus_tag="F603_RS0131260"
                     /pseudo=""
     CDS             8355..>9160
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000047: Methylation (70% identity)"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005486715.1"
                     /locus_tag="F603_RS0131260"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="cobalamin-dependent protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKSAGSAGRVMLVSMPWNNVRRPSIQVGTLRAVAEEEGWQVTPEY
                     AYLAFYGLAKRMLGYGDAEWSDAYDLISEGLYHFSVGDWIFACRGTEAARRDEYVEELR
                     SRKIDEPTIGLIDSLREVAERHVEETAAALLASDPDVIGFTSTFSQNGPTLAVAERLRA
                     SGYPGVIVMGGSNCEGSMGRALLENYPAVEAVVDGPGEDAFVALLRQVAAGGPVRSQGR
                     LLTRRPAGGADAAESGTGISLTGLSVPTPNYDGYFEQLHAAGLEGL"
ORIGIN
        1 cacgaaaccg tcctgcaagg cgacgacgcc gcactcttcc gggctgtcat cggcgggacc
       61 cgcatccgtg acctgcaagc caacggttgg ccggaaggcc aaccctggga ccgagcctgg
      121 gagaaactcc agaactggca acgccgccgc tggatcttca tcgaaggcac caaaatcatc
      181 gccctcgcca tccgcgaaca accatccgcc taccggacgc cgccgcccaa gggcacaccc
      241 aggcgacagg cccgcaaccc cgtgcccgtc acgctcacca gccggaagtg agggaggtga
      301 ggcctatcgc ggtgcatcca catgaacagc gcgacgacgg atggatcggt acagcgaaac
      361 gaaaggatca ctgacatggg accagcggtc gtattcgact gtatgacggc ggacttcctc
      421 aacgacgacc ccaacaacgc cgagctcagc tccctggaga tggaggaact cgagtcgtgg
      481 ggcgcctggt ccgacgacac cgaccagtca gtctgatgcc cgcctgcgcg tagcaccaga
      541 cgtgaagtcg gcgggccggc gcacagggaa gtgggagtga tggccgattc ttccgcgacc
      601 gactgcgtac tgcgggaagt cgcgttccgc tcctaccggg cggagcacac ggtgacggtc
      661 cagtgcacgg tacggcctga ctcgggaccg gggcaggccg aaggctacgg caccgccacg
      721 accgaggccg tcgctcggaa gaaggccctt tccgaagccg tggaaaggct gttggcctgc
      781 accccgttcg ccctcatcgc ccgtcggccg acaccgcgca cagaccggag caggggcacc
      841 gacccctggc agcgcgggct gcgctccgcg cccgccggct gccaggtacg caactaccgc
      901 tcgctgagcg gcgagcagcc gcggcaggtg cccctgttct ggtcttcgcc ctgggtcgcc
      961 ggcgaggaga tgcggtcggc gaacctcgcc ccgcaggcgg cccgcctctc cagcacggtc
     1021 gggtgggccg tcgcaccgtc gcccgaagca gccctgcagg gcgccctcta cgagctgacg
     1081 gagctgctga actacggcgc gttcctctac cggtgcctcg cggccccccg cgaacgcccc
     1141 gcgcccggcg gaggccccga gccgcacgaa gagggccgca cccagaccgt ccccataagc
     1201 tgggcgaccc gcaccccgac gatcctcgcc gtgacgagga gcagcgaccg gctgatgccc
     1261 gccaccggta tcggctcagg tacgacggcg tccgaggcgg ccgagcgggc cttcctcgaa
     1321 ctcgcccagg ccgagacgct ctggcgcgcc aactcgaccg ccgtgccggc cgagcgctac
     1381 ttcatgcggc gcttcgagaa gtggcccctg ctgagacgct gcgccgccct ggacttcgac
     1441 ctcggcgagc acgccggccc cttcgacgac gggccccacc cctctccctt gggcgcgctg
     1501 gagtccagag ccatcgaggt gtgggccgac cccggagccg tcgacatctc gggcgccgac
     1561 ctgaggagga cccgcctgtg cttcgcccac gtggtgtcgg atccgcagcc gctcctcggc
     1621 cttgtccgcg caggtattcc cgtcttcgac acgggggaag tgaggaagat ccttgtctgt
     1681 ccacgccgag cacgatctgc tgacctcagc ccttctggac gggtcgctca ctcaggacgt
     1741 cccacccgag cgtgagacgc cgatcggcag agcgctctcc gcggcgagcg actggctgag
     1801 ctccgagcaa ctgaccccgg acatctggaa gtacgggctc gactccgcgc ccacctacga
     1861 ggtccacctc aaggacgccg cgggcacgct gtgctcgcgc agctcgggca agggtctggg
     1921 gcaccgcagc atggcgagcg ccctcttcga aagcgccgag cactaccacc tcgactggcg
     1981 ccgcgacccg ctcgccgacg aggccgcgtt cgtccccggc cgcgaggtcg ccgggcagcc
     2041 gacggccgcc ggctcggcgc tgctgcaccg actcggcggc atcctcggcg actccccggt
     2101 cctcacccgc gaataccgcc cagtggccga ggcgttgggc gccaccggcg gcgccgcacc
     2161 cccgcaccgc caccccgtct tcctgcgcga cggcggctac cggaactggc cgcacccctc
     2221 ggacgacggc tccttcaagc cgctctggca ctacacgacg agcgccggct acgcggctgg
     2281 aaccacgctg cacgaggcgc tcgtccacgc ggtcaacgag ctgatcgagc gcgacgcctg
     2341 gtcctaccaa cttgcccgtt cctacttcgg cctgcccgac gagagcggcc gggccctccg
     2401 cgtcatcgac cacgagagcc tcccggcgga gctgcgcgcc ctcgtcgacg acgtggaggc
     2461 gtcgagcggc tccccgatcc tcatcgtctc gatcccctgc gacacggggg tgccggccta
     2521 cgtggtctgc gacgcggaga gcgacgagga cgtgcggctc atcggcagcg gcgcctcgcc
     2581 ggtgcaggcg tacgccctcc aacgagggct gctggagtac ctccaggtgt gcacgatgta
     2641 ccagcacggg cccgtcgacg cggagcgcga ggccgcccag atcgccacgg ccctggcccg
     2701 ctacccgagg cacctcgccg ccgccgggtt cgacgtgcgt caactcccgc acgaacggca
     2761 gccgttcgac agcgacgggc tccctgccgc ggcgactccg gaagagctgc tgcggcacct
     2821 catcgaccgg ttgcagggag tgggcgtcga cctccacttc cgcgtcctga cgaggccggg
     2881 gacgatcacc gtcgtcgacg tcgtcgcacc cgggctcgaa atgctggaca aggcgcgcgc
     2941 cggctacccg gtcctgccga ccgggcggtt gggcgagagg ctcaggccgc agaggaggga
     3001 gccgtgaagc tcgtccttgt ggcgccaccg tggaacagcc tctaccggcc ctcgatccag
     3061 atcgccaccc tcgccgcgct gagcaccgag gagcagggcg atccgatcag cgccgtctac
     3121 gggtacttgg actggttcga gtacgcggtc ggcttcctgg agtgcgaccc ggagcagttc
     3181 gccgccgtgt acgacacgat cggcgaggag ctgtacgggc tcggcgtcgg cgactggatc
     3241 ttcggcggcg ggctgccggg cgccggggac gcggacgagg cgtcggcgac cgcggagtac
     3301 ggtgcgttcc tgcgggagca gggcgtcgac gaggaaatcg tcgccgatgt cctgcggttg
     3361 cgcgaggcgg cgccacggtt cgtcgccgac ctcgccgagc ggctcgtgga cgacggggcc
     3421 gagtgcttcg gattcaccac atccttcagc cagttggtgc cctcgctggc gatagccgtg
     3481 gcgatcaagg aacgggcgcc ggaccggctc gtcctcctcg gcggggcgaa ctgcgaccgt
     3541 ccgatgggcc aggccgtgat gcacgcctat ccgttcgtcg acgcggtcgt cgagggcgag
     3601 ggcgagcagg cggtgaacgc gctcacccgc gcgaagagcg tggacgacct ggcggcggtc
     3661 cccgggatag tgctgcggca cgaggacggc ctgcgcaccg cgctgccgtc gatccaaccc
     3721 gtggagtccg ctcccgtgcc caagtacgat gcgtacttcg agcggttgga gagcctgccg
     3781 tggggcgacc tcatcgctcc gcacgtggcg ctgccgttcc agatgtcccg cgggtgctgg
     3841 tggggcgaga agttgcagtg cacgttctgc ggtgagaacg gcagcgggat ggtctaccgc
     3901 agcaaaccgg gctcccaact cgtcgacaca ctgcggacgt tgacggagcg gcacggcgcc
     3961 ttcaacacct acgccgtgga cacgatcctg gcgaagaacg acaacggcct cgcggcgctc
     4021 gccgacgccg aggtcgacat caccaccttc ttcgaggtca aggctaacct cggccctcgg
     4081 gaggtcggcg agctgcgcgc cgccggcgtc accatggtgc agccggggat cgagagcctc
     4141 agctcgggcg cgttgaagct gctgcgcaag ggcgcgacga gctggcacaa cctgcgcttt
     4201 ctcgtcctgt gccaggagtt ggggatcgac gcgcagtgga acttgctctc ggccatgccg
     4261 gacgagtcag cggagatgct ggaggagcag ctccggctga tccccttcat cacacatctg
     4321 gcaccaccgt cgacggtgag caaagtccgc gtcgaccgct acagccccta cttcgagaag
     4381 cccgacgagt tcggcatcga gctgcggggc cccgagaaga agtaccggtt catccaccgg
     4441 ggcgagggcg tcgaccgtac ggcgctggcg tactccttcg agcacgggca gcaggcgggg
     4501 aaggagcggg acgatgacgc agccatgctg gcgctgcgcc gccggctcgg ggcacgtgtc
     4561 ggactgtgga agaagctgca cgcgcagtcc cgcttctcct tccgtttcgg cccgaacgtg
     4621 accgtggtcc acgactacag gccgcacgtc ggtacgggcc gcaccgtgct gcggggagcc
     4681 gacgacctcc tcttccgctc cctcgtccac ggcgggcgcc tcatgcaggc cgtcggccgg
     4741 gtcggcgagc agacggggac gccggagagc cggctgcacg agagggtgag cgagtgggcg
     4801 gcgtgcggct gggtccacct tgaggggcgg cgcggtctcg tcctcgccgt acgggacggc
     4861 atgcgggacg agttgggccg cgcggcggcg ctgagggggt cggatgcgga gtcctgaccg
     4921 ggagcaggcg gaacgcttcg aagtcgcctc cgtggacggc acgttgctgc gcgggttcag
     4981 ccgggcggcc cgcgggtcgt ccgatgcgcg gggcgagccg gagacgctgg tgctgctgca
     5041 cggtgtcgcg atggaccacc gggtctggtc cgagtcgtcg ttcctcgacg cgctgcccga
     5101 cgacgtccac gtcgtcgccc ccgacctgcg cggccgcggc gaaagcgacc cggggcacag
     5161 cgccgagggc cacgcgatcg accgctacgt cgaggacgtc cgcgccgtgc tcggcttctt
     5221 cgggcgggag cggtacagcc tcttcggcct ctacttcggg ggccgtatcg cgctgcgggt
     5281 cgcggaggcg gacaggcggg tgcagcgggc cttctccttc tgcgcgcacg gcgaggcggt
     5341 ggaggtcccg gcggacgccg tcgaggagga ggcgaaagag atcgaaggcc ccggcgggta
     5401 cgcgtacctc cgcgaccact tctccgccgc gggagcgcct gcgtggatgg tctccgcctg
     5461 cgaccgggtc gaccaggacg agttggcggc ggcgacccgc ggcctcctgc acggttcgca
     5521 ccagcgggcc gagcgcggag cagccgacca ggacctggtg ctgatcactg ccgagggcga
     5581 cgccgacctc ggcacgttcc gcgagggctc ggcccgcctc ggcgcccggc tctggctggt
     5641 ggacagccgg acccgggtgc gcgccgctgc ccgcctcgcc gaagtcggca cgcgcacggc
     5701 ggagttgctc accgcggcgc cggcggcgat gaggggggac gcatgacgga cgacgccctc
     5761 tggacccacc gcaggggcct gctccccgac ggcgatccgc tcgtcggcgc gctggaggag
     5821 ctggcgggcc gcagccgcag ccacgtcctc cgcgccgtcg ggatgctcga cgcggagacc
     5881 ggagaggccg ccccgccaag ggacttggag gtggtcgacg gcgccgtcgc ccgctggagc
     5941 cctgcgccgg acgccgcgta ctgcgagtgg ttcgccctgc ccgggttcgt cgacgcccat
     6001 gcgcacgtgt cctcggtgac cgacctcgtc ggcatgctcg cgtacggggt gacgggttac
     6061 cggcagatgt ggggcgagcc cgcacacctg tacagcgccg gcgtccaccg tgcgcgccac
     6121 gccgtcctcc ccctcccctg gacgacggcg ggcgtcgtcg acggccccga ctcccgtatc
     6181 ccgcaccacg tcacggtcgt tgacgacgcg cggcaggtgc gccgggtggt cgaggacgtc
     6241 ctcgcgttcg gcttcgacgg catcaaggtc tacgacgact tggcgcggcc ggccttcgag
     6301 gcgctggtac gcgaggcgga gcgcgccggc atccccgtcg tcggacacgt gcccgaggcg
     6361 gtgccgctcg acgcggccta cccgggcatg tggtcgaccg agcacctgta cggaatcgtg
     6421 ccccacatct tccggctgcc cgcggccgac cgctgggccg tcctcgccga cgcgttgcag
     6481 cggcaccgcg acgacggccc cgccgtcgag ggggccgccg gccgtttcgt ctgcccgacg
     6541 ctcaccgcgt ggcgggcgcg gagcggggag cgccgctaca cccggccgtc gaggacgctc
     6601 ctccagtcgg cgaccccgag ccgccgcccc gcctggcacg cggccgcgag ggacgccctc
     6661 tcgctggagc gggccgaggc gcagcggcgg agcctgctgg tcgaccggct cggaggcatc
     6721 gcgtacgacc tgtcgcgccg cggggccagg ctgctcgtcg gcaccgactg cgggaacccc
     6781 ttcgtcctcg ccgggccctc gtaccacaag gagctggccg aactcagccg cagcggcctc
     6841 gacttcgcga ccgtcctcaa ggccgcgacc gtcgacgcat acgccgcaac gggccgcgac
     6901 cccgcacggg ccgacctggt cctctaccgg cgctccccgg cgcacgacgt cacccgcctg
     6961 gcccggccgg acgccgtact cgtcgacgga gtcctcctcg acgccgacga cttggaccac
     7021 ctgtgggggc tgcggctcac ggcggccggc ctcgacgccg gcacctggag aaggggcgac
     7081 gtggacccgg cggccggtga ccgacagaat aaggagactg ccgatgcagg ctgacccatc
     7141 caccgggacg accgaccccg aggctccccc cggggtctgc cccgtcaccg gacggcgcgc
     7201 cgaccccgtg gacttcgagt tcttcaacgg cccttcggcg taccgcgaag cagcggccac
     7261 ccacgccgac gagggcgcct tctacagcgc cagcggcgac gggttctggg tccttaccac
     7321 gtacgaggga atctgcgagg cgttcaggga cgaggagcag ttcaccgtcg ggcgcgtcag
     7381 cgcagcggac ggcgccgagg aggaccgctg gattccgctg accatcgagg gccgccagca
     7441 cacggagtgg cgccgcaggc tgggcgcgtg gttcacgccg cagcgcgtac gcgagctgac
     7501 cccctcgatc gaggccaacg cccggcgccg gatcgagggg ttcgtcgacg acggcgaggt
     7561 gtcgttcaac gaggatttcg ccaggccgtt cgtgctggag aacctgatga ccgcggtggg
     7621 ctgggagttc gaccgcttcg accaactcct gcgcatcgac cgcgcgatga tcgattcgcg
     7681 gtacgcgccc gacccgcgtg aggcggcgtt cgaggaggtc ggcatcccgg cgctggaaaa
     7741 gctcgcccgc gagcaggtcg agcgccgtcg cgccgaaccc gcagacgacc tcaccaccgc
     7801 gaccttcggc tgggaggtcg aggggaggcc cgtcaccgac gacgaccggg tctcgctgct
     7861 gtgcgtgctc ttcctcgccg gcgtcgactc gacggtcaac cacctggcca acgccgtcca
     7921 gcacctggtg tgccacgacg ccgacaagcg gcggttcctg gcctccaggg aagtacgccc
     7981 gagggcggtc gaggagttcc tccgggccaa ctcgtgcatg tatccgggga ggttggccgc
     8041 gcgcgacggg gcgggcggca ccgcccggcg gggtgagacg gtgctgctgc cgctcgccgt
     8101 cgccaactac gatcccgagg tcttccacga ccccgagcag gtcgacttcg accgggagcg
     8161 caacccgcac atcgccttcg gtacgggagc acaccagtgc ctcggcgccg cgttggcgcg
     8221 ggcgcagatc cgcaccgccc tcgacgtgtg gcacgagctc atcccgcact acggattccc
     8281 tccgcagccg gagccctcgg agccccggtt cctccgcaat gcatacgact tgaggctcgt
     8341 ctggtgaggt cgccatgaag agtgccggca gtgctggtcg agtaatgctc gtttcgatgc
     8401 cgtggaacaa cgtgcgccgc ccttcgatcc aggtgggcac gttgcgtgcg gtggccgagg
     8461 aggaaggctg gcaggtcacc ccggaatacg cctacctcgc cttctacgga ctcgccaaaa
     8521 ggatgctggg ctacggagac gccgagtggt cggacgcgta cgacctgatc tccgagggcc
     8581 tttaccactt ctcggtcggc gactggatat tcgcctgccg gggcacggag gccgcgcggc
     8641 gcgacgagta cgtcgaggag ctgcgctcca ggaaaatcga cgagccgacg atcgggctga
     8701 tcgattcgct gcgcgaggtc gccgagcggc atgtggagga gaccgccgcg gccctgctcg
     8761 cgtccgaccc cgatgtcatc gggttcacga gcaccttcag ccagaacggg ccgacgctgg
     8821 cggtggccga gcggttgcgt gcaagtggct atcccggcgt gatcgtcatg ggcggtagca
     8881 actgcgaggg ctcgatgggg cgggcgctcc tggagaacta ccccgcggtg gaggcggtgg
     8941 tcgacggtcc gggtgaggat gcgttcgtgg cgctgctgcg gcaggtggcg gccggcggcc
     9001 ccgtcaggtc gcaggggcgg ctgctcaccc gccgtccggc agggggagcg gatgcagccg
     9061 agagcggcac ggggatctcg cttaccgggc tcagcgttcc cacgcccaac tacgacggat
     9121 acttcgagca gttgcatgcg gcgggtttgg agggtctgga
//
