LOCUS       NZ_KB905830            10318 bp    DNA     linear   CON 02-MAY-2024
DEFINITION  Streptomyces sulphureus DSM 40104 F603DRAFT_scaffold00018.18, whole
            genome shotgun sequence.
ACCESSION   NZ_KB905830
VERSION     NZ_KB905830.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sulphureus DSM 40104
  ORGANISM  Streptomyces sulphureus DSM 40104
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 72882)
  AUTHORS   Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K.,
            Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A.,
            Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name :: Streptomyces sulphureus DSM 40104
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: DSM 40104, ATCC 27468
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. R37654
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi11971
            Current Finishing Status          :: Level 1: Standard Draft
            Type Strain                       :: Yes
            Funding Program                   :: DOE-CSP 2012
            Sporulation                       :: Sporulating
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. R37654
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000381025.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 01:54:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,193
            CDSs (total)                      :: 6,120
            Genes (coding)                    :: 6,022
            CDSs (with protein)               :: 6,022
            Genes (RNA)                       :: 73
            rRNAs                             :: 6, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 37 of 98
            Pseudo Genes (incomplete)         :: 75 of 98
            Pseudo Genes (internal stop)      :: 11 of 98
            Pseudo Genes (multiple problems)  :: 20 of 98
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:55:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 62564
            Orig. end                         :: 72882
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB905830.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405920
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Nikos  Kyrpides (nckyrpides@lbl.gov)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-PGF and JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10318
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RRE-containing"
                     /region_number="1"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /tool="antismash"
     cand_cluster    1..10318
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /kind="single"
                     /product="RRE-containing"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10318
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[10000:>10318](-)"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..>10318)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
     gene            1675..1863
                     /locus_tag="F603_RS33220"
     CDS             1675..1863
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016908998.1"
                     /locus_tag="F603_RS33220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_019549664.1"
                     /transl_table=11
                     /translation="MGIADQFKGKAEEMQKKAKDKMNEGGDRAKDTGQEAKEQGSRETK
                     SHESGKGEKKMRGSNDE"
     misc_feature    complement(2101..2116)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.08"
                     /tool="antismash"
     gene            2204..2365
                     /locus_tag="F603_RS36145"
     CDS             2204..2365
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="F603_RS36145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_019549665.1"
                     /transl_table=11
                     /translation="MPESGFDTDIRELGLFAVEWRQATRPGRRARLAFLCAGPEIAPPE
                     EARDCWND"
     gene            2435..2632
                     /locus_tag="F603_RS36150"
     CDS             2435..2632
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015835.4"
                     /locus_tag="F603_RS36150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_245600947.1"
                     /transl_table=11
                     /translation="MAGHCTTEPDGQLSLSATVFSRDGSKFTRAHIWGDTFNEAGTLGF
                     RVCAEPLRQDARDVIDGIPH"
     gene            3033..3611
                     /locus_tag="F603_RS0130240"
     CDS             3033..3611
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909005.1"
                     /locus_tag="F603_RS0130240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_019549667.1"
                     /transl_table=11
                     /translation="MSQQTLACFGYDDWAWLNPPREASVTADRVRVVADPGTDFPSGRG
                     QRTTGHALLRPAPLRFGIRAVVTGAYREQYDQAGLLLWCGAHHWVKAGIEYVDGVEQLG
                     AVVTRVSSDRSAVPLSRLRENSGPLTIALAREGPTVEIRFGLAGGEPRTLLRQAYFPPE
                     PECRTGPMVASPTGAGFAAEFSGVRFRSP"
     gene            complement(3618..4418)
                     /locus_tag="F603_RS0130245"
     CDS             complement(3618..4418)
                     /EC_number="2.1.1.-"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1292: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019549668.1"
                     /locus_tag="F603_RS0130245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SAM-dependent methyltransferase"
                     /protein_id="WP_019549668.1"
                     /transl_table=11
                     /translation="MAASEAEGKPLIDETVPHSARIWNYWLGGKDNYAVDRQAGDEYRR
                     AFPGVDELARQARHFLIRTIRYLAEDAGVRQFLDIGTGLPTVDNTHEVAQRIAPDARVV
                     YVDNDPLVLAHAHALLTSTPEGVTQYVDADLLDPEAVLAKASGTLDLRRPVGLILSGVL
                     GHVSSHGEAVRVVRTLVDALPPGSYLSLHDATDEDAGANEAADSYAETGAVPYLLRTRA
                     QIAAFFDGLEAVPPGLVPITQWHPEHQGGPAAETTGFGGVALKR"
     gene            complement(4543..5097)
                     /locus_tag="F603_RS0130250"
     CDS             complement(4543..5097)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909007.1"
                     /locus_tag="F603_RS0130250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_019549669.1"
                     /transl_table=11
                     /translation="MEIAQEAVRLFTAKGVAATSGEEIAAASGVSTRTLWRYFATKEQC
                     VRPLLTVALDIMAQVLRRWPAERPLRDAIGGEGWPQAHDPGDSRTLRDLVRLTGNEPGL
                     RAVWLEVHFAAEAVFAEILAERSRAPQPDPLALKVQAVMLNGALRVAVEDWAWRADESS
                     ELSGDESVRRALRLAASNLPL"
     gene            5308..6078
                     /locus_tag="F603_RS0130255"
     CDS             5308..6078
                     /EC_number="1.1.1.-"
                     /NRPS_PKS="Domain: PKS_KR (10-172). E-value: 2.7e-09.
                     Score: 29.1. Matches aSDomain:
                     nrpspksdomains_F603_RS0130255_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948181.1"
                     /locus_tag="F603_RS0130255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_019549670.1"
                     /sec_met_domain="adh_short (E-value: 5e-33, bitscore:
                     106.7, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSNRDFEGRSVIVTGAGSGIGRAAALKFAERGAKVLVADLSEEN
                     AHRVVGEVRDTGGTAHAVVGDLGLQEIVDEVTATALREFGRIDVLVNNAGIMDRMSAAA
                     DTEDAEWERVLRINLTAPFMLARAVVPHMIEAGGGAVVATASEASFRGSAAGAAYTASK
                     HGVAGLTKSLAVMYRDKGVRANAIAPGGTVTGISVDAQQGAHGPGVVGSYMVNMGSRTA
                     RAEEQAEAIVFLASDAASNINGVVLPVDNGWAAV"
     CDS_motif       5338..5421
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F603_RS0130255_0001"
                     /evalue="2.20E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="F603_RS0130255"
                     /protein_end="38"
                     /protein_start="10"
                     /score="20.3"
                     /tool="antismash"
                     /translation="SVIVTGAGSGIGRAAALKFAERGAKVLV"
     aSDomain        5338..5823
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F603_RS0130255_PKS_KR.1"
                     /evalue="2.70E-09"
                     /label="F603_RS0130255_PKS_KR.1"
                     /locus_tag="F603_RS0130255"
                     /protein_end="172"
                     /protein_start="10"
                     /score="29.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="SVIVTGAGSGIGRAAALKFAERGAKVLVADLSEENAHRVVGEVRD
                     TGGTAHAVVGDLGLQEIVDEVTATALREFGRIDVLVNNAGIMDRMSAAADTEDAEWERV
                     LRINLTAPFMLARAVVPHMIEAGGGAVVATASEASFRGSAAGAAYTASKHGVAGLTKS"
     CDS_motif       5680..5736
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_F603_RS0130255_0002"
                     /evalue="4.80E+00"
                     /label="PKSI-KR_m1"
                     /locus_tag="F603_RS0130255"
                     /protein_end="143"
                     /protein_start="124"
                     /score="0.0"
                     /tool="antismash"
                     /translation="LARAVVPHMIEAGGGAVVA"
     gene            complement(6139..7518)
                     /locus_tag="F603_RS0130260"
     CDS             complement(6139..7518)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016909009.1"
                     /locus_tag="F603_RS0130260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine hydrolase"
                     /protein_id="WP_019549671.1"
                     /transl_table=11
                     /translation="MTTSEARESLLPATARALLHRVAVAQAEGRAPSFVGGLMRGGALV
                     WSAGRSADEHRVPDADTQYRIGSLTKMFTAVLVLRLREEGLIDLAEPVAAHLPEAAPAG
                     SATVAQLLAHTSGLAAETGGPWWERTPGEVRPRLTDVFGEEPLLHPAGRTHHYSNPGYA
                     ILGALVAQVRGTTWERALRREILAPLGMERTTTAPEPPHAEGWAVHPFADVMQPEPAVD
                     TGRMAPAGELWSTAADLARFAGFLLDGDPRILPVETLREMRAPASGPNDPNWSAGYGLG
                     FGLVRTEGRVLFGHGGSMPGFLASLWVSEEDALAGIALANATSGPATGDVACDLVRLTR
                     DHEPRIPEPWRPASEADPELLALTGTWYWGTRAHTLRVTSGRNLSLTALDGGGRASRFR
                     PEPDGTWRGLDGYYAGERLRPVTTAEGTYLDLGSFVFTRAPYEPSAVLPGGADPRGWSG
                     A"
     gene            complement(7938..9380)
                     /locus_tag="F603_RS0130265"
     CDS             complement(7938..9380)
                     /EC_number="3.4.11.1"
                     /GO_function="GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000048: Hydrolysis (69% identity)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013003255.1"
                     /locus_tag="F603_RS0130265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="leucyl aminopeptidase"
                     /protein_id="WP_019549672.1"
                     /transl_table=11
                     /translation="MVRVSVDDSGPGACAKPADAVALPRYAGAPASTFCRRRGFLARAG
                     QVVAEPSGDGSVLLNVGLGAAGSATPATFRAAAAAAVRAAGPARTLRLDLALADGPVPP
                     AERARAVTEGALLALYRYDTHRSRKPARTLDELIVAVPQPEAAAAVREAQAVSDGTCLA
                     RDLVNAPPGELTPTLFADRARQMAEEYGFSCTVHEGAALAELGLAGLTAVGRGSREAAK
                     YVEIDYAPEGAPAVPVVSLVGKGVTFDSGGLSLKQAREMHTMKSDMGGAAAVVGALASL
                     ARLALPVRVRSHLPLTENMPDGAALRTGDVVRHLDGTTTEIVNTDNEGRLVLADVLVHA
                     GRARPDLLIDVATLTSAAAHALGTRTGALFTNDEQLAETVLASADRTGEPLCRLPLAED
                     ERRHLHSATADRANCSYRYGDTTQAALFLQDFVPTGLPWAHLDIAAPAHNDEPSYREVP
                     HGGTGFGVRTLVETLRRVSGGE"
     gene            complement(9384..9920)
                     /locus_tag="F603_RS0130270"
     CDS             complement(9384..9920)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839044.1"
                     /locus_tag="F603_RS0130270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF2087 domain-containing protein"
                     /protein_id="WP_019549673.1"
                     /transl_table=11
                     /translation="MNTASVEDQPPSCRDLVGTLAQSDRRSAYAALALGANSVGEVAQR
                     TGLRAPAAAAALQKLVEGKLARLDPETRGYSLIEDAFQRAMQAEVQVSGRAGGDGSGSY
                     FRRGRLTSIPGKPDVRARVLAVVADSFERGTVYSEAKVNALCGEWYDDWVSLRRALVDD
                     GLLVRDESCTAYQLA"
     gene            complement(10001..>10318)
                     /locus_tag="F603_RS34500"
                     /pseudo=""
     CDS             complement(10001..>10318)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Bottromycin_Methyltransferase_RRE"
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000049: Methylation (66% identity)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298726.1"
                     /locus_tag="F603_RS34500"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /sec_met_domain="Bottromycin_Methyltransferase_RRE
                     (E-value: 2.4e-29, bitscore: 93.5, seeds: 12, tool:
                     rule-based-clusters)"
                     /transl_table=11
                     /translation="HETVLQGDDAALFRAVIGGTRIRDLQANGWPEGQPWDRAWEKLQN
                     WQRRRWIFIEGTKIIALAIREQPSAYRKPPAKGTPRQARTPVPVTLGAKPPPSWQPEGK
                     A"
     aSDomain        complement(10145..10315)
                     /aSDomain="Bottromycin_Methyltransferase_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing methyltransferase protein in a
                     bottromycin cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_F603_RS34500_Bottromycin_Methyltransf
                     erase_RRE.1"
                     /evalue="1.00E-29"
                     /identifier="RREFam008.1"
                     /label="F603_RS34500"
                     /locus_tag="F603_RS34500"
                     /protein_end="57"
                     /protein_start="0"
                     /score="93.5"
                     /tool="antismash"
                     /translation="HETVLQGDDAALFRAVIGGTRIRDLQANGWPEGQPWDRAWEKLQN
                     WQRRRWIFIEGT"
ORIGIN
        1 tggctgcggt atccgatctc gggggcgctc ggcgagactt tcgccaaccc ggccgacacc
       61 tcggggcgcc ccacgctcga gcacttcgac ctcgccaccg cgcgccgcac cgaactcacg
      121 ggcgacgtcg actggttcga ggtgagcggc gacggcaccc gcctcgtggt gagcgacgag
      181 ggggagctgc gcgccgtccc cgcggacgag agcgcggacg ccgactcgac gacccgcatc
      241 gacctgcggc gcatcctcca cgacgtcgac ccggccgcgg agtggcggca gtcgtacgcg
      301 gaggcaggcc ggttgatccg cgcgtacttc tgggacccgg ggatgagcgg cgtcgactgg
      361 gacgcggtgc tcgcgcagta ccgcccgctc gtcgaacgta tcgccacccc cgacgagttc
      421 gccgacctcc tccgcgaggt cctcggcgag ctcggcacct cgcacgcctc cgtggcggcc
      481 tcgcgccgca gcgaggggcc gccgcactac cgtcgcccgc tcggcttcct cggcgtcgac
      541 gccgtgcgca cggtggaggg ccgctggcgg atcgagcgca tcctgccggg caactcctcg
      601 gactcccggg cgcgttcacc gctcgcgggg acggccgtgc gcgaggggga gtcgatcgtc
      661 gccgtcgacg cgcggaaggt cgatccggtg gccggacccg cgccgctcct cgctgcggcc
      721 ggcggcacca ccgtcgaact cacctgtgcg caggacgacc cggacggcgg ggaaccgcgg
      781 ctgcaccgcg tcgccgtcgt ccccctcatc gacgagcggc agttgcgcta ccaggactgg
      841 atcgcccggc ggcgtgagaa ggtgcgggcg ctcagcggcg gcaagtgcgg ctacctgcac
      901 atccccgacc tcggcggttc gggctgggcg cagttcaacc gggacctgcg gatggagatc
      961 tcgcggcccg cactcctcat cgacgtgcgg ggcaacgccg gcggcaacat cagcgagttg
     1021 gtgctggaga agctgacgcg ctcggtgctc ggctgggacc tgacgcgcaa cgcgcagccg
     1081 gtctcgtaca cgacgaacgc cccgcgcggc ccgctcgtcg ccctcgcgga cgaggcgacg
     1141 tcctcggacg gcgacatgat cacggcggcg ttccggctgc tcggactcgg acccgtggtc
     1201 ggcgcgcgga cgtggggcgg cgtcgtcggg atcaccgggc ggcaccggct cggcgacggc
     1261 acggtgatca cggtgccgat gaacgcggcg tggttcgagg ggtacgggtg gtcggtcgag
     1321 aaccacggtg tcgatcccga catcgaggtg gtccgcacac ccgtcgactg ggccgagggc
     1381 cgcccgtcgg agctggaggc cgccgtgcag acggcccttg agctgctgga gcggcacccc
     1441 gccgccgtac ctcccgacta ccgcgacgta ccggaccgga gcaggccgcc gctgccgccc
     1501 cgcacgtgac ggacacaccg tgcaggagcg aacacacagc gcaaattggc gcgttggtgt
     1561 ggtccgtgcc acgctgaaaa aggtgggtta cggacataac gcacatgtgg gccagagcag
     1621 cccgtgcccc ctgccgaagg ggccacccca cgccgtcagc gtcgaggagt gacgatgggc
     1681 atcgcagacc agttcaaggg caaggccgag gaaatgcaga agaaggcgaa ggacaagatg
     1741 aacgagggag gggaccgcgc caaggacacc ggccaggagg ccaaggaaca gggctcccgc
     1801 gaaacgaaga gccacgagtc cggcaagggc gagaagaaga tgcgcggcag caacgacgag
     1861 tgacgccccc tcgggcccgt cacccgcaca cacccgtcac cgaccggtca acgtcccttc
     1921 gcccgaccgc agtcggcaga cgggacccgc gccggggccg tttggtcccg gcgttgcggt
     1981 gtgctccacc ggcttgcggc agcgcctggg cagacggccg taagcggggg caggaggagc
     2041 cgccatggca tcgaacatgc gtcgtttccc ctggcggccg actcgctgag agcgtcgtac
     2101 tgttcgcgca tggtgtcggc atcgctcaag gggtaggcag atggcacctc tccggcggga
     2161 tcgttgaaca ccacgagtca ccactcgatg ccgtccgtcg agaatgccgg agtcagggtt
     2221 cgacaccgac atacgtgaac tcggcctgtt cgccgtcgag tggcggcaag ccactcgccc
     2281 cggccgccgt gcccgcctcg cgttcctctg tgcaggaccc gagatcgcac cacccgagga
     2341 agcacgcgac tgctggaacg actgaaccac cccggaaccc ggacggaggt gaccgcggaa
     2401 cgtccacggc ctgcgcggac actgcaactc cccgatcgcc ggacactgca ccaccgaacc
     2461 cgacggccaa ctcagcctca gcgcaacggt gttctcccgc gacgggagca agttcacacg
     2521 cgcgcacatc tggggcgata ccttcaacga ggccggcacc ctgggcttcc gcgtctgcgc
     2581 cgagccgctg cggcaagacg cccgcgacgt catcgacggc attccgcact gactcggcgc
     2641 tcctcagcga ccgctccaag aggatgcact ccgcggctgg ttgctccaac cataggcttc
     2701 ctctgtgagc acccgtttac gatctcttgc cgggacaccc ggtcaggccc ggagaacgcc
     2761 cgatgcgggc gggcacttcc gacctctcgg ctctccaacc gactattcct acggtttccg
     2821 ttccgagagt cgtgcgcccc accccgcccg aaaacgcacc ggggccgcgg tgaccgagcc
     2881 gcagccccgg tgcgcgcgca ccctctcaca ggccgacgcc gcgcgtccgg ccaagtcccc
     2941 ctgaacgtac ggctgctcgg ccgcacccgc gggcgtacgg tgacggcagc ggagcccgtc
     3001 ggaccccgcc agccggaccc gggaggccga ccatgtccca gcagactctc gcgtgcttcg
     3061 ggtacgacga ctgggcgtgg ctcaacccgc cccgtgaagc gtccgtcacc gccgaccgcg
     3121 tccgcgtcgt cgccgatccg ggcaccgact tcccttccgg ccgcgggcaa cgcacgacgg
     3181 ggcacgccct gctgcgtccc gcaccgctgc ggttcgggat acgcgccgtc gtcacaggtg
     3241 cctaccgcga gcagtacgac caggcggggt tgctgctgtg gtgcggtgcg caccactggg
     3301 tgaaggccgg catcgagtac gtcgacgggg tggagcagct cggcgccgtc gtcacccggg
     3361 tctcctccga ccgcagcgcc gttccgctct cccgactccg ggagaacagc ggcccgttga
     3421 cgatcgcgct cgcccgcgag ggccccaccg tcgagatccg cttcggactc gccggcggcg
     3481 aaccccgcac cctgctccgg caggcgtact tcccgcccga gcccgagtgc cgcacaggtc
     3541 ccatggtcgc ctcgccgacg ggtgcggggt tcgccgccga gttcagcggg gtgcgcttcc
     3601 ggtcgccctg agcggggcta cctcttcagc gccacaccgc cgaagcccgt cgtctcggcc
     3661 gccgggccgc cctggtgctc cgggtgccac tgagtgatcg gtacgagccc cggcgggacg
     3721 gcctcgaggc cgtcgaagaa ggcggcgatc tgcgcgcggg tgcgcaggag gtagggcacg
     3781 gctccggtct cggcgtagct gtcggccgcc tcgttggcgc ccgcgtcctc gtcggtggcg
     3841 tcgtggaggg agaggtagct gcccggtggg agcgcgtcga cgagtgtgcg gacgacgcgg
     3901 accgcctccc cgtgcgagga gacgtgcccg agcacgccgc tgaggatcag tccgacgggg
     3961 cgcctgaggt cgagcgttcc gctcgccttc gccaacacgg cttcggggtc gaggagatcc
     4021 gcgtcgacgt actgggtgac gccctcgggg gtgctggtga gcagcgcgtg cgcgtgagcg
     4081 aggacgagcg ggtcgttgtc gacgtagacg acccgggcgt cgggcgcgat gcgctgcgcg
     4141 acctcgtggg tgttgtccac ggtggggagc ccggtgccga tgtcgaggaa ctgccgcaca
     4201 cccgcgtcct ccgccaggta gcggatggtg cggatgagga agtggcgggc ctggcgggcg
     4261 agttcgtcga cgccggggaa ggctctgcgg tactcgtcgc cggcctggcg gtcgacggcg
     4321 tagttgtcct tgccgccgag ccagtagttc catatccgcg cggagtgcgg cacggtctcg
     4381 tcgatcaacg gtttgccctc ggcttctgaa gctgccatcg gcggccttcc tcctcgatac
     4441 gtacgcgcgg cggccttctg ccgccggccc cagcagtctg cccgggaagg cccggcgtgt
     4501 gcggaccgaa cgggcctttc cgccagggga gttcgccggc gctcagagcg gcaggttcga
     4561 ggcggccagg cgcagggcgc gccggacgga ctcgtccccg ctcagctccg acgactcgtc
     4621 ggcgcgccag gcccaatcct cgacggcgac gcggagggcg ccgttgagca tcaccgcctg
     4681 caccttgagg gcgagcgggt ccggctgcgg cgcacgggag cgttcggcga ggatctcggc
     4741 gaagaccgcc tcggcggcga agtggacctc gagccacacg gcgcgcagcc ccggctcgtt
     4801 accggtgagc cggacgaggt cgcgcaacgt ccggctgtcg cccgggtcgt gggcctgcgg
     4861 ccacccctcg ccgccgatcg cgtcgcggag cgggcgctcg gccggccaac ggcggaggac
     4921 ttgggccatg atgtcgaggg ccaccgtcaa cagcgggcgt acgcactgct ccttggtggc
     4981 gaagtagcgc cacagggtgc gggtggagac tccggacgcc gctgcgatct cctcgcccga
     5041 ggtggcggcc acgcccttgg cggtgaagag gcggaccgcc tcctgcgcga tctccacacg
     5101 cgtcgccgct ttgcgcctct cgctcagcgg cggccttccc gtccgaccgc tgttcgccct
     5161 cttctcagcc atggcggcca ccttaagtcc ggcgccggtt tgtcgcctca tgccgtcgtg
     5221 gcactcactg ccaataagtt gtacggtcgg ccgcacggac ggatcgcggg caccggccgg
     5281 gaaccgagca aggcaaggga gcccctcatg agcagcaacc gcgacttcga aggtcgcagc
     5341 gtcatcgtca ccggcgccgg gtccggcatc gggagggcgg ccgcgctgaa gttcgccgag
     5401 cggggcgcga aggtcctcgt cgccgacctc tccgaggaga acgcgcaccg cgtcgtcggc
     5461 gaggtgcggg acaccggcgg gacggcgcac gcggtcgtcg gcgacctcgg cctccaggag
     5521 atcgtcgacg aggtgaccgc caccgcactc cgcgagttcg gccgcatcga cgtgctcgtc
     5581 aacaacgccg gcatcatgga ccgcatgtcc gctgccgccg acaccgagga cgccgagtgg
     5641 gagcgggtgc tgcggatcaa cctcaccgcg ccgttcatgc tcgcgcgcgc ggtcgttccc
     5701 cacatgatcg aagcgggcgg gggcgccgtc gtcgccacgg cctccgaggc gagcttccgc
     5761 ggcagcgcgg ccggcgcggc ctacaccgcc tccaagcacg gggtcgccgg actgaccaag
     5821 tccctcgccg tgatgtacag ggacaagggc gtgcgggcca acgcgatcgc gcccggcggc
     5881 accgtcacgg gtatctccgt cgacgcgcag cagggtgcgc acggtcccgg tgtcgtcggc
     5941 tcgtacatgg tgaacatggg ctcgcgcacg gctcgggccg aggagcaggc cgaggcgatc
     6001 gtcttcctcg cctcggacgc cgcgagcaac atcaacggcg tcgtccttcc cgtcgacaac
     6061 ggctgggcgg ccgtctgagc gccggcgctt ctccggggag cgacccgaac cccactgttt
     6121 cgcggggccg ttggggagtc aggcgccgct ccagccccgc gggtccgcgc cgcccggcag
     6181 cacggcgctg ggctcgtagg gggcgcgggt gaagacgaag ctgccgaggt cgagataagt
     6241 tccctcggcc gtcgtgacgg ggcgcagccg ctcgcctgcg tagtaaccgt cgaggccgcg
     6301 ccaggtaccg tccggctcgg ggcggaagcg ggaggcgcgg cctcccccgt cgagggcggt
     6361 gaggctgagg ttgcgccctg aggtgacccg cagggtgtgc gcgcgggtgc cccaatacca
     6421 ggtgccggtg agcgcgagga gctcgggatc tgcttcggat gccgggcgcc agggctccgg
     6481 gatgcggggc tcgtggtcgc gcgtgagccg caccaggtcg caggcgacgt cgccggttgc
     6541 cggacccgag gtggcgttgg cgagggcgat gccggcgagg gcgtcctcct cgctcaccca
     6601 cagcgaggcg aggaagccgg gcatggagcc gccgtggccg aagaggacgc ggccttcggt
     6661 gcggaccagg ccgaagccga ggccgtaacc cgctgaccag ttggggtcgt tggggcccga
     6721 ggccggggca cgcatctcgc ggagcgtctc gacggggagg atgcgagggt cgccgtcgag
     6781 gaggaagccg gcgaagcggg cgaggtcggc cgccgtcgac cacagctctc ccgcgggcgc
     6841 catccggccc gtgtcgacgg ccggttcggg ctgcatgacg tcggcgaagg ggtggaccgc
     6901 ccagccctcg gcgtgcggag gctcgggagc cgtggtcgtg cgctccatgc cgagcggggc
     6961 gaggatctct cgccggaggg cccgctccca ggtggtgccg cggacttggg cgaccagggc
     7021 gccgaggatc gcgtatccgg ggttggagta gtggtgggtg cggcctgcgg ggtggagaag
     7081 gggctcctcg ccgaaaacgt ccgtgaggcg ggggcgcacc tcgcctggcg tccgctccca
     7141 ccaggggccg cctgtctcgg ctgcaagccc cgaggtgtgc gcgaggagtt gggcgacggt
     7201 cgcgctgccg gcaggtgcgg cctccgggag gtgggccgcg accggttctg cgagatcgat
     7261 caggccctcc tcgcgcaggc gcaggacgag gacggccgtg aacatcttgg tgagcgagcc
     7321 gatgcggtac tgggtgtcgg cgtccgggac cctgtgctcg tcggcgctgc ggcccgccga
     7381 ccagacgagc gcacccccac gcatcaggcc cccgacgaac gacggcgccc tgccctcggc
     7441 ctgggccacg gcgacgcggt ggagcagtgc cctggcggtg gcgggcagaa gcgattcccg
     7501 ggcttcgctg gtggtcatgc gcccactcta cgagcaccct ccgacacggg ccggggagtg
     7561 cgtcaacggt cgtggggtga gggggtgctt ggccaggtgg cccgcgggtg gagtgtcacg
     7621 cgccgcgtgg agtcggtgcg tggctgggac gagcgcgccg tcacgtggat gcggccggtt
     7681 gtcggaattt gcctggcacc gcgcgcagtt gggacacgtg acgcaacacg cagaggcagc
     7741 acataaccgc gcgaggctct cgcctaccgc gacgtgggcg gctacgccgc cgcggtcctc
     7801 gccggcgtgc tcagacgcac tcccccgact ggggccggtg tgagtgcgag gtgcaccctc
     7861 gccgccgacg cgaccagacg gcgagccccg ccgattgacc gtcgctcaac gcggcatgcg
     7921 cccaggtgag ttggaggtta ctcgccgccc gatacccgcc gcagcgtctc gaccagggtg
     7981 cgtacgccga agcccgtgcc gccgtgcggg acctcgcggt aggacggctc gtcgttgtgg
     8041 gcgggggccg cgatgtccag gtgggcccag ggcaggccgg tcgggacgaa gtcctggagg
     8101 aagagggcgg cctgggtcgt gtcgccgtac cggtacgagc agttggcgcg gtcggctgtc
     8161 gcggagtgga ggtgccggcg ctcgtcttcg gcgagcggga gccggcacag tggttcgccg
     8221 gtgcggtccg ccgaggcgag caccgtctcg gcgagctgct cgtcgttggt gaagagggcg
     8281 ccggtgcggg tgccgagggc gtgggcggcg gccgaggtga gggtggccac gtcgatcagt
     8341 aggtcggggc gtgcgcgtcc ggcgtggacg aggacgtcgg cgaggacgag gcggccctcg
     8401 ttgtcggtgt tgacgatctc cgtggtggtg ccgtcgaggt ggcgtacgac gtcgcccgtg
     8461 cgcagggcgg cgccgtccgg catgttctcc gtcagcggca ggtgggagcg gacgcgtacc
     8521 ggcagcgcca gtcgggccag cgaggcgagt gctcccacta cggcggcggc accgcccatg
     8581 tcggacttca tggtgtgcat ctcgcgcgcc tgcttgaggg agagcccgcc cgaatcgaag
     8641 gtgacgccct tgcccacgag gctcaccacg gggacggcgg gggcgccctc cggcgcgtaa
     8701 tcgatctcca cgtacttcgc ggcctcgcgg gagccccggc cgacggcggt gaggccggcg
     8761 agccccaact ctgcgagcgc cgcgccctcg tggaccgtgc aggagaagcc gtactcctcc
     8821 gccatctgcc gggcccggtc cgcgaagagc gtgggggtca gctcgcccgg tggggcgttg
     8881 acgaggtcgc gggcgaggca ggtgccgtcg ctgacggcct gggcctcgcg cacggctgcc
     8941 gccgcttcgg gctgcggaac ggccacgatc agctcgtcga gggtgcgcgc cggcttcctc
     9001 gaccggtggg tgtcgtaccg gtagagggcg agcagggcgc cctcggtcac cgcgcgtgcg
     9061 cgttccgcgg gcggcacggg gccgtcggcc aaggcgaggt cgaggcggag ggtgcgtgcg
     9121 gggcccgcgg cgcgcacggc tgcggcggcc gccgcgcgga aggtggcggg cgtcgccgaa
     9181 ccggcggcac cgaggccgac gttgagcagc acactcccgt caccggacgg ctcggcgacg
     9241 acctgcccgg ctctggcgag gaatccgcgg cgtcggcaga acgtgctcgc cggggcgcct
     9301 gcgtaccgcg gcagcgcgac ggcgtcggcc ggcttcgcac atgcgcccgg cccgctgtcg
     9361 tcgaccgaga cgcggaccat gcgtcaggcg agttgatagg ccgtgcagga ctcgtcgcgt
     9421 acgagcaggc cgtcgtcgac caaggcgcgc ctgaggctta cccagtcgtc gtaccactcg
     9481 ccgcacagcg cgttgacttt cgcctcggag tacacggtgc cccgctcgaa cgagtcggcg
     9541 acgacggcga ggacgcgggc gcgtacgtcc ggcttgccgg ggatcgacgt cagtcggccg
     9601 cggcggaagt acgagccgga accgtccccg ccggcccggc cggagacctg cacctcggcc
     9661 tgcatggcgc gttggaaggc gtcctcgatg agggagtagc cgcgcgtctc cgggtcgagc
     9721 cgggcgagct tgccctccac cagcttctgc agggccgccg cggcggccgg cgcgcgcagc
     9781 cccgttcgct gggcgacctc tcccacggag ttggccccga gggcgagcgc ggcgtaggcg
     9841 gaccgccgat cggactgcgc gagggtgccg accaggtcgc ggcaggaggg cggttggtcc
     9901 tctacggagg ccgtgttcat ggcggtatcc accttccgga tcgtgaggtc gcgcgtagcg
     9961 taaccgcgat ggactgggcg acgcacggga attcgcgcga ctatgccttt ccttcaggct
    10021 gccaggaggg tggcggcttg gctcccaggg tgacgggcac gggggtgcgg gcttggcggg
    10081 gtgtgccttt tgcgggtggt tttcggtagg cggatggttg ttcgcggatg gcgagggcga
    10141 tgattttggt gccttcgatg aagatccagc ggcggcgttg ccagttctgg agtttctccc
    10201 acgcccggtc ccagggttgg ccttccggcc aaccgttggc ttgcaggtca cggatgcggg
    10261 tcccgccgat gacagcccgg aagagtgcgg cgtcgtcgcc ttgcaggacg gtttcgtg
//
