LOCUS       NZ_KB905815            10405 bp    DNA     linear   CON 02-MAY-2024
DEFINITION  Streptomyces sulphureus DSM 40104 F603DRAFT_scaffold00003.3, whole
            genome shotgun sequence.
ACCESSION   NZ_KB905815
VERSION     NZ_KB905815.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Streptomyces sulphureus DSM 40104
  ORGANISM  Streptomyces sulphureus DSM 40104
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 890774)
  AUTHORS   Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K.,
            Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A.,
            Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name :: Streptomyces sulphureus DSM 40104
            collection_date                   :: Missing
            lat_lon                           :: Missing
            depth                             :: Missing
            alt_elev                          :: Missing
            country                           :: Missing
            num_replicons                     :: Missing
            ref_biomaterial                   :: DSM 40104, ATCC 27468
            biotic_relationship               :: Missing
            rel_to_oxygen                     :: Missing
            isol_growth_condt                 :: Missing
            assembly :: Velvet v. 1.1.04; ALLPATHS v. R37654
            finishing_strategy                :: Missing
            environment                       :: Missing
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi11971
            Current Finishing Status          :: Level 1: Standard Draft
            Type Strain                       :: Yes
            Funding Program                   :: DOE-CSP 2012
            Sporulation                       :: Sporulating
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. R37654
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000381025.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 01:54:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,193
            CDSs (total)                      :: 6,120
            Genes (coding)                    :: 6,022
            CDSs (with protein)               :: 6,022
            Genes (RNA)                       :: 73
            rRNAs                             :: 6, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 37 of 98
            Pseudo Genes (incomplete)         :: 75 of 98
            Pseudo Genes (internal stop)      :: 11 of 98
            Pseudo Genes (multiple problems)  :: 20 of 98
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:55:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 53960
            Orig. end                         :: 64365
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB905815.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405920
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Nikos  Kyrpides (nckyrpides@lbl.gov)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-PGF and JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            21..422
                     /locus_tag="F603_RS0108320"
     CDS             21..422
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="F603_RS0108320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_019545550.1"
                     /transl_table=11
                     /translation="MPARPLALCAAAALAALSVLVAPAHATAEQTSPVRHRAASACLSH
                     AVSYNTKADSFIPHPRGTYRASSRCKDINLRIASGGATKVCVRFHPGPDCARKVRVGAK
                     WKVIASNVRNGARFQVVFDGQFHKGRIAY"
     gene            complement(426..830)
                     /locus_tag="F603_RS0108325"
     CDS             complement(426..830)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907225.1"
                     /locus_tag="F603_RS0108325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF1992 domain-containing protein"
                     /protein_id="WP_019545551.1"
                     /transl_table=11
                     /translation="MTERKPPGISFESWADRQIREAEQRGEFDDLPGKGKPLRSLDQPY
                     DEYWWLREKAERESLSYLPPALVLRKEAEQVREQAVTARSEAEVRASVREVNEKIRAAI
                     RNPPEGPPLNVAPLDEERLVAVWRERRPSA"
     gene            complement(878..1969)
                     /locus_tag="F603_RS0108330"
     CDS             complement(878..1969)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907224.1"
                     /locus_tag="F603_RS0108330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amidohydrolase family protein"
                     /protein_id="WP_019545552.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 2.3e-16, bitscore:
                     55.5, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVESGTLHITGRVLVGPEDVRDELWVVDGRITFERPGTAAGAQTR
                     LHGWVLPGLVDAHCHVGLDAHGAVPEQQSEDQALTDRGAGALLLRDAGSPADTRWIDDR
                     DDLPRIIRAGRHIARTRRYVRNYAHEIEPGDLAAYVRNEAKRGDGWVKIVGDWIDREEG
                     DLSACWPRWAIEEAINAAHEEGARVTAHCFAEDSLRDLVEAGIDCIEHATGLTEDTIPL
                     FAERGTAIVPTLVNIATFPGLADGGEAKYPRWSAHMRRLHARRYETVRAARDAGISVYA
                     GTDAGGSLAHGLVGEEVAELVRAGIPATEALSAAAWGARSWLGRPGIEEGASADLVVYP
                     EDPRADVRVLTAPEHVVLRGVVR"
     gene            1968..>2203
                     /locus_tag="F603_RS36370"
                     /pseudo=""
     CDS             1968..>2203
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /locus_tag="F603_RS36370"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGAPYEQRCAAGYTVRNSAGLVGRQRGVDGGVQVLGHGTVRGVAT
                     AADPGPVDRDLLPGAWCPFCNVAFRALRKHLPR"
     gene            2285..2494
                     /locus_tag="F603_RS36375"
     CDS             2285..2494
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="F603_RS36375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_019545553.1"
                     /transl_table=11
                     /translation="MRVNGGHPRTPSIPATYLLDRNGTVRWSFVDTDCTRRAEPAELLV
                     RLDELKAEERRMPQGSGEGGKTPR"
     misc_feature    2668..2685
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.57"
                     /tool="antismash"
     misc_feature    complement(2670..2685)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2680..2695
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(2831..2847)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     gene            2903..3415
                     /gene="ectA"
                     /locus_tag="F603_RS0108340"
     CDS             2903..3415
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019545554.1"
                     /locus_tag="F603_RS0108340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_019545554.1"
                     /transl_table=11
                     /translation="MPEGLKLDAPRVADGAAIWRIARDSGTLDLNSSYSYLLWCRDYAA
                     TSVVARDEAGTPVGFITGYIRPDHPETLLVWQVAVDEAARGRGLAAAMLDGLSARTARE
                     LGTAGIETTVTPDNIPSNRLFTSYAERHGAPVAKEVLFEAEAFPEEGHEPEVLYRIGPV
                     ALADERR"
     gene            3536..4804
                     /gene="ectB"
                     /locus_tag="F603_RS0108345"
     CDS             3536..4804
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-365). E-value: 2.8e-73.
                     Score: 238.8. Matches aSDomain:
                     nrpspksdomains_F603_RS0108345_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="F603_RS0108345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_019545555.1"
                     /sec_met_domain="Aminotran_3 (E-value: 6.7e-77, bitscore:
                     254.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRAQASHMYDEDGHAY
                     LDFFAGAGTLNYGHNNPVLKRALLDYLERDGVVHGLDMSTSAKRAFLETFKSTILEPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDDYLDGKVDDFLWFERLLEDQGSGLNKPAAVIVESVQGEGGINVART
                     EWLRALSDLCTRHDMLLILDDIQMGCGRTGAFFSFEEAGIKPDIVTLSKSIGGYGLPLA
                     LTLLKPELDVWEPGEHNGTFRGNNPAFVTATAALETYWTDGQMEKQTISRGEQVEAALK
                     AIADEHPGEIADFRGRGLVWGLEFEEKARADRVAKRAFEHGLLIETSGPQSEVIKLLPA
                     LTIAPEELDEGLRILARSVREAA"
     aSDomain        3623..4630
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_F603_RS0108345_Aminotran_3.1"
                     /evalue="2.80E-73"
                     /label="F603_RS0108345_Aminotran_3.1"
                     /locus_tag="F603_RS0108345"
                     /protein_end="365"
                     /protein_start="29"
                     /score="238.8"
                     /tool="antismash"
                     /translation="DRAQASHMYDEDGHAYLDFFAGAGTLNYGHNNPVLKRALLDYLER
                     DGVVHGLDMSTSAKRAFLETFKSTILEPRDLPYKVMFPGPTGTNAVEAALKLARKVKGR
                     ESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDDYLDGKVDDFLWFERL
                     LEDQGSGLNKPAAVIVESVQGEGGINVARTEWLRALSDLCTRHDMLLILDDIQMGCGRT
                     GAFFSFEEAGIKPDIVTLSKSIGGYGLPLALTLLKPELDVWEPGEHNGTFRGNNPAFVT
                     ATAALETYWTDGQMEKQTISRGEQVEAALKAIADEHPGEIADFRGRGLVWGLEFE"
     gene            5001..5405
                     /locus_tag="F603_RS0108350"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="F603_RS0108350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_019545556.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.4e-55, bitscore:
                     180.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHIKSASGTWESKRIVLAKEKVGFSLHETILY
                     AGTETDMWYANHIEAVLCVEGEAELTNRETGEKHWITPGTMYLLNGHEKHTMRPKTDFR
                     CICVFNPPVTGREDHDENGVYPLLTEPDEG"
     gene            5477..6376
                     /gene="thpD"
                     /locus_tag="F603_RS0108355"
     CDS             5477..6376
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="F603_RS0108355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_019545557.1"
                     /sec_met_domain="PhyH (E-value: 1.4e-38, bitscore: 128.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGATEVATPRMDPVVWSEPGTSGPIEASDLA
                     GFERDGFLAVDQLLTPDEVAECGRELGRLTSDPEIRADERSIVEPKSDEIRSVFEVHKI
                     SEVFAKLVRDPRVLDRARQILGSDVYVHQSRINLKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYDTNGGLMIMPGSHKTFLGCAGETPRDNYKKSLQMQDAGTPSDEA
                     LTKFAEAHGIKLFTGKPGSATWFDCNCMHGSGDNITPYSRSNVFIVFNSVENTAVEPFA
                     APVRRPEFIGARDFTPVK"
     gene            complement(6444..7964)
                     /locus_tag="F603_RS0108360"
     CDS             complement(6444..7964)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907218.1"
                     /locus_tag="F603_RS0108360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_019545558.1"
                     /sec_met_domain="Aldedh (E-value: 1.1e-44, bitscore: 148.3,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWSVDPRTGNRREQVTEEATAADVDAAVQAAHRAGDSLADRAGRA
                     AFLRAASAELLDAQGEIVPVADAETALGTARLDGEMARTAAQLRAFADELDDGAYLGAT
                     VDHADPQRIPPLPDLRRVKMPLGVVAVYAASNFPLAFSVPGGDTASALAAGCPVVVKAH
                     PDHPATSQLCAAALRRAAASVGLPAEVLTVVHGFDAGIALVEHPLVAAAGFTGSVRGGR
                     ALFDAAAARPRPIPFHGELGSLNPVVVTEQAADERPDEIGAGLAGSVTQGTGQFCVKPG
                     LVLVPEGAAGDGLLASLGEALAAAPAGPLLDARMREAFLAGVRRRAGLEGVEAPVAAGE
                     GAAENTVAAGFLTVPARRLAEPGPHELLLEECFGPVTVVARYRDAEEAGAVLAVLPGNL
                     TATVHVGAEEAGEEKGRGAELLARLAPLAGRLVVDGWPTGVAVAPAQHHGGPYPATTST
                     STSVGATAIERWLRPVAYQNTPEPLLPPELRDGNPLGLPRRVDGRREG"
     misc_feature    8062..8075
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.25"
                     /tool="antismash"
     gene            8095..8865
                     /locus_tag="F603_RS0108365"
     CDS             8095..8865
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381154.1"
                     /locus_tag="F603_RS0108365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_019545559.1"
                     /transl_table=11
                     /translation="MAADGTGGSQVKSAVRTVELLEYFAGRPGMHSLAAVQEAVGYPKS
                     SLYMLLRTLVDLGWVETDLTGTRYGIGVRALLVGTSYIDGDEVVAAARPTLDRLSDDTT
                     ETIHLARLDGTNVVYLATRQSQHYLRPFTRVGRRLPAHSTSLGKALLATHSDDEVRALL
                     PAELPQLTEHTHTDREKLVEELHAIRARGFAVDREENTLGLRCFGVAVPYRTPARDAIS
                     CSVPVARLTSAHEQTIQAALLDARDRLTLATRRL"
     gene            complement(8962..9849)
                     /locus_tag="F603_RS0108370"
     CDS             complement(8962..9849)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907216.1"
                     /locus_tag="F603_RS0108370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="WP_019545560.1"
                     /transl_table=11
                     /translation="MSVRALLTGWFSFLEGEATAGDVLALRRVEEVMRERRVPYDVVWS
                     PGFRPGALSFRDVEPGRYGLLLFVCGPLHGPQVAGLHEEYAHCRRLAVGVSVIDPGDPA
                     VRGFHEVIARDGPDVAPLADLSAGTRPPRTLPPVVGVVLTGGQQEYGGRRRHEGTAERV
                     TGWLARQDCARLPLETRLDDRDWRLCRTPEQWDAVLSRLDLVVTDRLHGLVLALRSGVP
                     ALAVDPVAGGAKVSAQAQAMRWPALVHAEQMDDATLAYWWEWVRTEGPARAAAHAASPP
                     PDLVAAALRSTLFA"
ORIGIN
        1 cctcccggga agggaccccg atgcccgctc gccccctcgc cctctgcgcc gccgcggcgc
       61 tggccgcgct ctccgtcctc gtcgcccccg cgcacgcgac cgcggaacag acctcgcccg
      121 tccggcaccg ggcggcatcg gcctgcctct cgcacgccgt ctcctacaac acgaaggccg
      181 acagcttcat cccccacccc cgcggcacgt accgcgcgtc gtcccggtgc aaggacatca
      241 acctgcgcat tgcctcgggc ggcgccacga aggtctgcgt ccgcttccac ccgggtccgg
      301 actgcgcacg caaggtccgc gtcggcgcca agtggaaggt gatcgcgagc aacgtacgca
      361 acggcgcgcg gttccaggtc gtcttcgacg gccagttcca caaggggcgg atcgcctact
      421 gacgctcacg ccgaggggcg gcgctcccgc cagacggcga cgagccgctc ctcgtcgagc
      481 ggagcgacgt tcagcggcgg gccctccggc gggttccgga tcgcggccct gatcttctcg
      541 ttcacctcgc gcacgctcgc ccgcacctcg gcctcgctgc gcgccgtcac cgcctgctcg
      601 cgcacctgct cggcctcctt gcgcaggacg agggcgggcg gcagatagct gaggctctcg
      661 cgctcggcct tctcacggag ccaccagtac tcgtcgtacg gctggtcgag gctgcgcagc
      721 ggcttgccct tgccgggcag gtcgtcgaac tcgccgcgct gctcggcctc cctgatctga
      781 cggtcggccc aggactcgaa gctgatcccc ggcggtttgc gctcggtcat gatgcggccc
      841 cctggagtcg gtgacccgcc caaggatacg gcctccgcta acgcaccacc ccgcgcagta
      901 ccacgtgctc gggcgccgtc aacacccgta cgtcggcccg cgggtcctcc gggtagacga
      961 cgaggtcggc cgaggctccc tcctcgatcc ccggccgccc cagccaggac cgcgcgcccc
     1021 aggcggcggc ggagagcgcc tcggtggcgg ggatgccggc cctgacgagt tcggcgacct
     1081 cctcgccgac gaggccgtgc gcgagcgaac cgccggcgtc cgtccccgcg tagacggaga
     1141 ttcccgcgtc ccgcgccgcc cggaccgtct cgtacctgcg cgcgtggagc cggcgcatgt
     1201 gcgcggacca gcgcgggtac ttggcctcgc cgccgtcggc gagcccgggg aaggtggcga
     1261 tgttgacgag cgtggggacg atggcggtgc cccgttcggc gaagagcggg atcgtgtcct
     1321 cggtgagccc tgtggcgtgc tcgatgcagt cgatcccggc ctcgacgagg tcgcggagcg
     1381 agtcctcggc gaagcagtgc gcggtgaccc tcgcgccctc ctcgtgcgcc gcgttgatcg
     1441 cctcctcgat cgcccagcgc ggccagcacg cgctcagatc gccctcctcc cggtcgatcc
     1501 agtcaccgac gatcttcacc cagccgtcgc cgcgcttggc ctcgttccgg acgtaggcgg
     1561 cgaggtcgcc cggctcgatc tcgtgcgcgt agttgcggac gtagcgcctg gtgcgggcga
     1621 tgtggcggcc ggcgcggatg atcctgggga ggtcgtcgcg gtcgtcgatc cagcgggtgt
     1681 cggcgggcga gccggcgtcg cggagcagca gggcgccggc gccgcggtcg gtgagcgcct
     1741 ggtcctcgct ctgctgctcc ggtaccgcgc cgtgcgcgtc gaggccgacg tggcagtgcg
     1801 cgtcgacgag cccgggcagc acccaaccgt gcagccgcgt ctgcgcaccg gccgccgtcc
     1861 ccggccgctc gaaggtgatc cgcccgtcga cgacccagag ttcgtcccgt acgtcctcgg
     1921 ggccgacgag cacccgcccc gtgatgtgca gcgttccgct ctcaaccatg ggggcaccct
     1981 acgagcagcg gtgcgcggct gggtacacgg tacggaacag cgcgggccta gtcggccgcc
     2041 agcgcggcgt cgacggaggt gtgcaggtcc tcgggcacgg caccgtgcgc ggagtcgcaa
     2101 ctgctgctga cccggggccc gttgatcgtg accttctacc gggcgcctgg tgccctttct
     2161 gcaacgtcgc gttccgcgca ctccggaagc acctgccgcg gttcaccgaa cgaggggccc
     2221 aactcgtcgc cgtctccccg cagatacccg acgagtcgcg tacgagcagc tcggcttcga
     2281 tctggtgcgc gtcaacggcg gccatccgcg cacaccgtcg atcccggcga cgtacctcct
     2341 cgaccgcaac ggcaccgtcc gttggtcctt cgtcgacacg gactgcaccc gccgtgcgga
     2401 gccggccgaa ctgctcgtaa ggctcgacga gttgaaggcc gaggagaggc gcatgcccca
     2461 agggtccgga gaaggcggga agacgccccg gtgaaccgca gtcaacttat tcgggaaaac
     2521 tctgtcctca ccgcacccca tacaatagag tgcattcggc ggcaggcttt cagagcacag
     2581 gttcccgttt tcttctgccc gctttcgccc acctcaaacg ccatgatttc aggggccgtt
     2641 agccggtaat tcggacgcct ctcacatcgg ttaccgcggt gtgacacatt ccacaacgcg
     2701 ccctttttgc ccaggcgaat ccggacatgg gcggtgatca gcgcgggggt ctgcgcgcct
     2761 gcgcgccccc gcgcgataac acaagacccc cgggacacgt aaccccgcac gcccgtgcag
     2821 tttgagattc tgctaggtaa attaaatccg catgaccgcc gcacaagcag aagatgcacg
     2881 tgcccgcagc gatttccggg aaatgccgga gggactgaag ctggacgctc cccgcgtggc
     2941 cgacggagcc gcgatctggc gtatcgccag ggactcgggg acgctcgacc tgaactcctc
     3001 gtacagctac ctgctgtggt gcagggacta cgcggccacc tccgtggtcg cccgcgacga
     3061 ggcaggcacc ccggtcggct tcatcaccgg ttacatacgt cccgaccacc cggagacgct
     3121 cctcgtctgg caggtagccg tcgacgaggc ggcgcgcgga cgcggactgg cggcggcgat
     3181 gctcgacggg ctctcggcgc gcaccgcccg cgagctcggc acggccggga tcgagaccac
     3241 ggtcaccccc gacaacatcc cctcgaaccg gctgttcacc tcctacgcgg agcgccacgg
     3301 cgcgcccgtc gccaaggagg tcctcttcga agcggaggcg ttccccgagg agggccacga
     3361 gcccgaagtg ctgtaccgga taggcccggt ggcgctcgcc gacgaacggc gctgaccacc
     3421 gccgcaggcg cgcgcccgac cgctgccctg ccccgaccgc gcaccgtcca ccgccgatgc
     3481 gccccaaaag ccccgatcac actcctcgca cacgacccac tccctggagc aagccgtgac
     3541 catcacccag cctgacctca gcgtcttcga gaccgtggag tcggaggtgc gcagctactg
     3601 ccgtggctgg cccaccgtct tcgaccgcgc gcaggcgtcc cacatgtacg acgaggacgg
     3661 gcacgcctac ctcgacttct tcgccggcgc cggaaccctc aactacggcc acaacaaccc
     3721 cgtgctcaaa cgcgcgctcc tcgactacct ggagcgggac ggcgtcgtgc acggcctcga
     3781 catgtcgacg agcgccaagc gggcgttcct cgagacgttc aagtcgacga tcctcgagcc
     3841 gcgcgacctg ccgtacaagg tcatgttccc cgggccgacc ggcaccaacg ccgtcgaggc
     3901 ggcgctgaag ctcgcccgga aggtcaaggg gcgcgagtcg atcgtctcct tcaccaacgc
     3961 cttccacggg atgtcgctcg gctccctcgc cgtcaccggc aacgccttca agcgggcggg
     4021 cgccggcatc ccgctcgtcc acggcacccc gatgccgttc gacgactacc tcgacggcaa
     4081 ggtcgacgac ttcctctggt tcgagcgcct cctggaggac cagggctcgg ggctcaacaa
     4141 gccggccgcc gtgatcgtcg agagcgtgca gggcgagggc ggcatcaacg tggcgcgcac
     4201 agagtggctc cgggccctct ccgacctgtg cacccggcac gacatgctgc tcatcctcga
     4261 cgacatccag atgggctgcg gccgcaccgg cgccttcttc tccttcgagg aggcgggcat
     4321 caagccggac atcgtcaccc tgtcgaagtc gatcggcggt tacgggctgc cgctggcgct
     4381 caccctcttg aagccggagc tcgacgtctg ggagcccggt gagcacaacg gcaccttccg
     4441 cggcaacaac ccggcgttcg tcaccgcgac cgccgcgctg gagacgtact ggaccgacgg
     4501 ccagatggag aagcagacca tcagccgggg cgagcaggtc gaggccgcgc tcaaggcgat
     4561 cgccgacgag cacccgggcg agatcgcgga cttccgcggc cgcggcctcg tgtgggggct
     4621 ggagttcgag gagaaggccc gcgccgaccg cgtcgcgaag cgtgccttcg agcacgggct
     4681 gctcatcgag acctccggcc cgcagagcga ggtgatcaag ctgctccccg cgctcaccat
     4741 cgccccggag gagctcgacg agggcctgcg catcctcgcg cgctccgtcc gcgaagccgc
     4801 gtagcaccgc ggttcccccg acgcgccgtc cccgcgcgag catcaggccc gcggtgacgg
     4861 tcgccaccgg cccgtcccgg taccacccgg ggcgggccgc ccccttgaga gggcacccgc
     4921 gcacgccgcc ccggcgccgc gacgggagag gagaaccggc ccgcccgggc cggacaccga
     4981 gagagaggca ccagcacacc gtgatcgttc gttcgttcaa ggacatcgag ggcaccgacc
     5041 gccacatcaa gtcggcctca ggcacgtggg agagcaagcg gatcgtcctc gccaaggaga
     5101 aggtcggctt ctcgctccac gaaacaatcc tttacgcggg tacagagacg gatatgtggt
     5161 acgcaaacca catcgaggcc gtactctgcg tggagggcga ggccgaactc accaaccgag
     5221 agaccggtga gaagcactgg atcacccccg gaacgatgta cctcctgaac gggcacgaga
     5281 agcacacgat gcgcccgaag accgacttcc ggtgcatctg cgtcttcaac ccgcccgtga
     5341 cgggccggga ggaccacgac gagaacggcg tctacccgct gctcaccgag ccggacgagg
     5401 gctgaccgca gttcgaggcc ggccggccgc accgcccatg tgacagggcc gtacgagagg
     5461 agaggaaggc acattcatga ccaccgcacc cgagcgcacc gccgacctgt acccgactcg
     5521 tggcgccacc gaggtggcga cgccgcggat ggaccccgtc gtgtggtccg agccgggtac
     5581 gtccgggccc atcgaggctt cggacctggc gggcttcgag cgggacgggt tcctcgccgt
     5641 cgaccagctc ctcacacccg atgaggttgc tgagtgcggc agggaactgg gacggctcac
     5701 ctctgacccg gagatccggg ccgacgagcg ctcgatcgtc gagccgaagt cggacgagat
     5761 ccggtcggtg ttcgaggtgc acaagatcag cgaggtcttc gcgaaactgg tgcgcgaccc
     5821 gcgcgtgctg gaccgggcgc ggcagatcct gggttcggac gtctacgtcc accagtccag
     5881 gatcaacctc aagcccggct tcggcgcatc gggtttctac tggcactcgg acttcgagac
     5941 ctggcacgcg gaggacggac tgccgaacat gcggaccgtc tcggtctcca tcgcgctgac
     6001 cgagaactac gacaccaacg gcggtttgat gatcatgccg gggtcgcaca agaccttcct
     6061 cggctgcgcg ggcgagaccc cgcgcgacaa ctacaagaag tcgctgcaga tgcaggacgc
     6121 cgggaccccg tccgacgagg cgctcaccaa gttcgccgag gcgcacggga tcaagctgtt
     6181 cacgggcaag cccggttcgg cgacctggtt cgactgcaac tgcatgcacg gaagcggtga
     6241 caacatcact ccgtactcgc ggagcaacgt cttcatcgtc ttcaacagcg tcgagaacac
     6301 cgccgtcgag cccttcgcgg ccccggtccg acgccccgag ttcatcgggg cgcgtgactt
     6361 caccccggtg aagtgacggg ctgagcccag gggatgtggg gcgggaccgc atgacggtcc
     6421 cgccccacgt tcgtgcgtac ggctcagccc tccctgcgac cgtcgacgcg gcggggcagc
     6481 ccgagcgggt tgccgtcgcg cagctcgggc gggaggagcg gctcgggggt gttctggtac
     6541 gccaccgggc gcagccagcg ctcgatcgcc gtggcaccga ccgaggtcga cgtcgacgtg
     6601 gtggccgggt aggggccgcc gtggtgctgc gcgggggcga cggcgacgcc ggtcggccag
     6661 ccgtcgacga ccaaccggcc ggcgagcggg gcgagtcggg cgagcagctc ggcgcccctc
     6721 cccttctcct cgcccgcttc ttcggcgccc acgtgcaccg tggctgtcag gttgcccggg
     6781 agcaccgcga gcacggcgcc ggcctcctcc gcgtcccggt accgcgcgac gacggtgacg
     6841 gggccgaagc actcctccaa cagcagctcg tgcgggcccg gttcggccag ccggcgggcc
     6901 ggcacggtga ggaacccggc ggccaccgtg ttctccgccg cgccctcgcc cgcggcgacc
     6961 ggcgcctcca cgccttcgag tccggcgcgg cggcgcacgc ctgcgaggaa cgcctcccgc
     7021 atccgcgcgt cgaggagcgg cccggccggt gcagcggcca gcgcctcacc gagcgaggcc
     7081 aacaggccgt cgcccgctgc gccttcgggg acgaggacga gccccggctt cacgcagaac
     7141 tggcccgtgc cctgggtgac cgaacccgcg agcccggcgc cgatctcgtc ggggcgctcg
     7201 tccgccgcct gctcggtgac gacgaccggg ttgagcgagc cgagttcgcc gtggaacggg
     7261 atcggacgcg ggcgggccgc cgcggcgtcg aaaagggcgc ggccgccgcg cacggaaccc
     7321 gtgaagccgg ccgctgcgac caacgggtgc tccaccaggg cgattccggc gtcgaagccg
     7381 tggaccacgg tgagcacctc ggccggcaac ccgacggagg cggccgctcg ccgcagggct
     7441 gcggcgcaga gttgggaggt ggcggggtgg tcggggtggg ccttgacgac gacggggcag
     7501 cccgccgcaa gagcgctcgc cgtgtcgcct cccgggacgg agaaggcgag cgggaagttg
     7561 gaggcggcgt agacggcgac gactccgagc ggcatcttga cgcggcgcag gtcggggagc
     7621 ggcgggatgc gctgcgggtc ggcgtggtcg acggtggcgc ccaagtaggc gccgtcgtcc
     7681 aactcgtcgg cgaacgcgcg cagttgcgcg gcggtgcggg ccatctcgcc gtcgaggcgg
     7741 gcggtgccga gagcggtctc ggcgtcggcg acgggcacga tctcgccctg tgcgtcgagg
     7801 agttcggccg aggccgcgcg caggaaggcg gcgcgccccg cacggtcggc gaggctgtcg
     7861 cccgcgcggt gcgccgcctg caccgcggcg tcgacgtcgg cggccgtggc ctcctcggtc
     7921 acctgctcgc gccggtttcc ggtcctgggg tcgacgctcc acactggaac tgtcaacgaa
     7981 agcctcctga cgggcggttg ggggtccagc cctctcgttc gatatgctga acaccatccc
     8041 ggtaagtgaa tatgcgggga ctgtatggcc gtgcgaacaa gggggtcaag gtcgatggcg
     8101 gcagacggga cgggtggttc gcaggtcaag tcggccgtgc ggacggtgga gttgctggag
     8161 tacttcgccg ggcggccggg gatgcactcg ctcgcggccg tccaggaggc cgtcgggtat
     8221 ccgaagtcga gcctctacat gctgctgcgc acgctcgtcg acctcggctg ggtggagacc
     8281 gacctcaccg gtacccgcta cggcatcggg gtgcgcgccc tcctcgtcgg cacctcctac
     8341 atcgacggcg acgaggtggt ggccgccgcc cgccccaccc tggaccgcct ctccgacgac
     8401 acgaccgaga cgatccacct cgccaggctc gacggcacca acgtcgtcta cctcgccacc
     8461 cgccagtcgc agcactacct ccgcccgttc acccgcgtcg gccgccgcct ccccgcgcac
     8521 tccacctcgc tcggcaaggc gctcctcgcc acccacagcg acgacgaggt gcgggccctg
     8581 ctgccggcgg agcttccgca gctcaccgag cacacccaca ccgaccgcga aaagctcgtc
     8641 gaggaactgc acgcgatacg cgcgcgcggc ttcgccgtcg accgcgagga gaacaccctc
     8701 ggactgcgct gcttcggcgt cgccgtcccc taccgcacgc cggcgcgcga cgcgatcagc
     8761 tgctccgtcc cggtcgcccg cctcacctcc gcgcacgagc agacgatcca ggccgcgctc
     8821 ctcgacgcga gggaccggct gacgctcgcc acccggcggc tgtgaaacgc gggcccggcc
     8881 gaactcctcg cccgatcggc cgcgttgggg ggcgcgacgt gcgctcacgc cccgcctgcg
     8941 cgtcacacca cgtcaaggcc ctcacgcgaa gagcgtggac cgcagcgccg cggcgacgag
     9001 gtccggcggc ggggaggcgg cgtgggccgc ggcacgtgca gggccctcgg tgcggaccca
     9061 ctcccaccag tacgcaaggg tggcgtcgtc catctgctcg gcgtggacga gggcgggcca
     9121 acgcatcgcc tgcgcctgcg cgctgacctt cgcgcccccc gccaccgggt cgacggcgag
     9181 tgccgggacg ccggagcgca gcgcgagcac gaggccgtgg agacggtcgg tgaccaccag
     9241 gtcgagccgg gagagcacgg cgtcccactg ctcgggggtg cggcacaggc gccagtcgcg
     9301 gtcgtccagc cgcgtctcca acggcagccg tgcgcagtcc tgtcgggcga gccagccggt
     9361 tacccgctcg gccgtcccct cgtggcggcg gcgacccccg tactcctgct gtccgccggt
     9421 gaggacgacc ccgacgacgg gcggcagcgt gcgcggcggc cgggtgcctg cggagaggtc
     9481 ggccaaaggg gcgacgtccg ggccgtcccg cgcgatcacc tcgtggaagc cgcgtacggc
     9541 cgggtccccg gggtcgatga ccgagacgcc gacggcgagg cggcggcagt gcgcgtactc
     9601 ctcgtggagc ccggcgacct gcggtccgtg caggggcccg cagacgaaca acagcaggcc
     9661 gtagcgcccg ggttcgacgt cacggaagga cagcgcgccc ggacggaacc ctggactcca
     9721 cacgacgtcg tagggcactc gccgctcccg catcacctcc tcgacacggc gcagcgcgag
     9781 cacgtccccc gccgtcgcct ccccctcgag gaagctgaac cagccggtga gcagcgcccg
     9841 gacgctcacg acgcgggccc ggggaagtgg acgggccgcg ggcgcggcag ggcctcgacg
     9901 gcctccacga cctcgtgggg agcgagccgg agcagccgcg ggtcgggcag cggaccgtgc
     9961 gggtcgccgg gggccgcgcc gtccagcggg ccggggcgcc acagcgggcg gtgcctgtcg
    10021 tgcggaggcg gtccccacac cgcgggcgag acgggcccga agaggacgac cgagggtgtg
    10081 gccagggccg aggcgaggtg ggccagcccc gtgtcgccga cgacgaccgc gcgcgcctcg
    10141 gccaccagcg tcgcgagcgt cccgaagggc aacccctcgg gaccgccgag caccgcgccg
    10201 cgaggcagtc cggcccgctc ggcgaccccg tgtgcgagcg ccccctcccg cgcaccgtgc
    10261 gtgacgacga cctcggcgcc ccggtcgctc aactcccgcg ccacggaggc gaaccggtcg
    10321 gcgggccagc gccgcgcccc cgcgtcggcg ccggggtgca cgacgacggc gccgggcgcg
    10381 ggcgaggccg cgcccgggcg gggca
//
