LOCUS       NZ_KB905458            18187 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Sciscionella marina DSM 45152 A37UDRAFT_scaffold_8.9, whole genome
            shotgun sequence.
ACCESSION   NZ_KB905458
VERSION     NZ_KB905458.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Sciscionella marina DSM 45152
  ORGANISM  Sciscionella marina DSM 45152
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Sciscionella.
REFERENCE   1  (bases 1 to 276736)
  AUTHORS   Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K.,
            Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A.,
            Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Sciscionella marina DSM 45152
            collection_date                   :: SEP 2006
            lat_lon                           :: Missing
            depth                             :: 516 m
            alt_elev                          :: Missing
            country                           :: China
            num_replicons                     :: Missing
            ref_biomaterial                   :: DSM 45152
            biotic_relationship               :: Free living
            rel_to_oxygen                     :: Aerobe
            isol_growth_condt                 :: 19196758
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Sand; Sediment; Marine; Aquatic
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi11892
            Current Finishing Status          :: Level 2: High-Quality Draft
            Type Strain                       :: Yes
            Funding Program                   :: DOE-CSP 2012
            Isolation Site :: Marine sand sediment at a depth of about 500 m,
            the northern South China Sea
            Cell Shape                        :: Rod-shaped
            Temperature Range                 :: Mesophile
            Temperature Optimum               :: 28 C
            pH                                :: 7
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000379465.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 01:37:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,209
            CDSs (total)                      :: 8,146
            Genes (coding)                    :: 7,961
            CDSs (with protein)               :: 7,961
            Genes (RNA)                       :: 63
            rRNAs                             :: 4, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 1 of 185
            Pseudo Genes (frameshifted)       :: 52 of 185
            Pseudo Genes (incomplete)         :: 155 of 185
            Pseudo Genes (internal stop)      :: 15 of 185
            Pseudo Genes (multiple problems)  :: 34 of 185
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:59:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 258549
            Orig. end                         :: 276736
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB905458.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405877
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Nikos  Kyrpides (nckyrpides@lbl.gov)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-PGF and JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..18187
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="3"
                     /rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..18187
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..18187
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[10000:14121](+)"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..14121
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     gene            395..1459
                     /locus_tag="A37U_RS52465"
     CDS             395..1459
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_transf_9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /locus_tag="A37U_RS52465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="WP_020502355.1"
                     /sec_met_domain="Glyco_transf_9 (E-value: 3.8e-06,
                     bitscore: 20.5, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKDSRNGRAELPVRFTVPNLSVRDCVELAAGLATYAQIHGIPQI
                     EVSGPPVIGELAQEFGFNELRFVDDHQQPGINIPAVPPSWRCGQRGLRCFSEQIADTTR
                     ALSPSDDVALSRLTAGSVMRFSRHNRLGDALGIVSGLEHYCRDNDVESVAVSGSHSLAE
                     VVEAFACDRVAHVSSSSEAIDGDAVFARSSWSVPWLSRFNLAVADIFGGRARHQTPPLR
                     LRAMQPPESRGSLVYCQFDARSSTPVPRDAARHVLSKLGSHGEVRVLGGPDTQAYLGED
                     ITYVRADLNEMIACLLSCRLFVGCDSGVGHLAGCLGVPGLIVSTDDFETTWSFFRGYSN
                     LNVIPMAAAELFLS"
     gene            1456..2406
                     /locus_tag="A37U_RS50830"
     CDS             1456..2406
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /locus_tag="A37U_RS50830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminoglycoside phosphotransferase family protein"
                     /protein_id="WP_338055199.1"
                     /sec_met_domain="APH (E-value: 1.4e-15, bitscore: 51.9,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPPTEQEVLRAVGRCLGTETATASRISMGRSHFVFTVALASGES
                     VIARITRHDRAECFTGFAYWRCKLAKVGVPVPRVLAMDLTETVVPWPVMVLERGAGDDL
                     CNVYAGLSDVEKQAVADDLLDIQHRVSTLPPGRGYGGVVGYHDKRCHGHWSGVLREYVA
                     GASSNLSGKKRQRPAIRAIETALSELQPEFDRIEPLPYLIDATHQNVLIHKGRVTAIID
                     LDELGFGDALYPLGTARTGLLARGPEADCIERVAAPLCATPLRRRIFALYSAIASLKML
                     APSPLGVTGPPAADSPISVRLHALLDEFAAAANEE"
     gene            2411..3553
                     /locus_tag="A37U_RS0140425"
     CDS             2411..3553
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /locus_tag="A37U_RS0140425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase"
                     /protein_id="WP_020502357.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 6.1e-08,
                     bitscore: 26.5, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGIVYVSIDDFSREGFTNGLFLQVRELLRRATARGQQASLACIA
                     PRMASGVERRTRTVSGCTIHETFVAPPGKASAYRAAFRELLDELAPDVILLNTCAIQLT
                     QAHHVVLRESLASGKRVVVLVTDQLYPTSDSHPDDEILRYYDLLRRTSVHAVSRTMAEA
                     FQKQTGVPAAVLPNLLPEPQTAPTTSDETGRHLTLINHHPIKGRRVWDALIRQRPHDAY
                     LVVETWPDVPPYTPPSPQVEIAGFAEDPSTIYNRTRILLVPSLGPEGLSRVTLETMESG
                     VPVVAHHIGSLSELESAAVLVTPPPIVGYELDGGVLYPVVSPSDIRRAALDFNERIDAL
                     DHDASFRERCVNSGRALVRNYRALSEAAADQLLDTWFAGT"
     misc_feature    2492..2494
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    2753..2755
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    3428..3430
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3696..4964
                     /gene="tyrS"
                     /locus_tag="A37U_RS0140430"
     CDS             3696..4964
                     /EC_number="6.1.1.1"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tyrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009057788.1"
                     /locus_tag="A37U_RS0140430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosine--tRNA ligase"
                     /protein_id="WP_020502358.1"
                     /transl_table=11
                     /translation="MTAIVDELKWRGLIAHTTDEDALHKALTDGPVIFYCGFDPTAASL
                     HVGHLVQVLTMRRLQQAGHRPLALVGGATGHIGDPRPTAERTLNDSETIAEWAIRLRAQ
                     IEPYLSFEGENAAMMVNNLDWTANMSVIEFLRDVGKHFRVNKMLTKDSVAQRLESDQGI
                     SYTEFSYQLLQGMDFLELYRRYGCTLQTGGSDQWGNLTAGLDLIHRLETDAEVHAFGTP
                     LMTKADGSKFGKSETGTLWLDPEMTTPYAFYQFWLNTDDRDIGRYLRVLSFKSREELLE
                     LEKETEEKPQARAAQHALAEELTTLVHGADQCDAAIAASQALFGQGELGDLDETTLTAA
                     VAELPKARVSEPGQVVDLFAEVGLVGSKSAARRTVKEGGAYVNNVKVTAEDAAVTVDQL
                     LHGRWLILRKGKKNLAAVEFTGA"
     misc_feature    3762..3764
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    3843..3845
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5060..5479)
                     /locus_tag="A37U_RS52470"
     CDS             complement(5060..5479)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A37U_RS52470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157183352.1"
                     /transl_table=11
                     /translation="MNQPPEHRREAHKRELMRAGRLLQDMLYNRASREQSPERIRITTA
                     ALRQAIHALESYGDTDSLDPREPLLVGPPRASEGAYWFIQHLGDRGVHRCEQTGVTIYA
                     DCGKVFSPKPANDGELALALPLRDFAQICRRCTQA"
     misc_feature    complement(5609..5627)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 20.13"
                     /tool="antismash"
     misc_feature    5610..5628
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 19.94"
                     /tool="antismash"
     misc_feature    5647..5660
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.09"
                     /tool="antismash"
     gene            6252..6659
                     /locus_tag="A37U_RS0140440"
     CDS             6252..6659
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A37U_RS0140440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_020502360.1"
                     /transl_table=11
                     /translation="MTAEQYLFIRSSFRLDQNPDEVARILQLSVETVLRHQPRANVPRH
                     ARGKHLTDELRSEIRTAWCAGLTLKQITREFEIGDQTALRYRPSELAPRQRISEIQESI
                     IVQGWRDKVNPSDIADRAGVGLTTVYKYRLR"
     misc_feature    6735..6751
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.41"
                     /tool="antismash"
     gene            6936..7439
                     /locus_tag="A37U_RS0140445"
     CDS             6936..7439
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A37U_RS0140445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_020502361.1"
                     /transl_table=11
                     /translation="MLTSVDVPVSEAVRRAIQKEAARESSPRTIAGKLGISVAQVMQHY
                     PHPEPQTFVIPPQPQRGPRDPLIAEQAHAMRIAWLAGWTSNEIATHFQVSKQTVLKYQP
                     WEILKLRELASTGTAPDWHELSGEVQLVILWAESLGWRVKVIASRLKLPAVAVRKYLAA
                     DYDR"
     misc_feature    complement(7462..7475)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.58"
                     /tool="antismash"
     misc_feature    complement(7924..7940)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.51"
                     /tool="antismash"
     misc_feature    8382..8394
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.63"
                     /tool="antismash"
     gene            8454..9713
                     /gene="fxlM"
                     /locus_tag="A37U_RS47770"
     CDS             8454..9713
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="fxlM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR04364.1"
                     /locus_tag="A37U_RS47770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methyltransferase, FxLD system"
                     /protein_id="WP_157183353.1"
                     /sec_met_domain="PCMT (E-value: 7e-35, bitscore: 114.7,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHFETRDAARGDGPDPADPDRVDEFRHAMVDDLLQDRTLYSTRVA
                     RAMRGVPRHLFASGVPLEEAYSDITLVTDRDEDGHALHVLSAPGVQATMLQQADVRPGQ
                     RVLEIGSGGYNAALIADLTGPDGEVTTVDTCPEQAARTRRDLDTAGYPHVQAVHAKVEQ
                     GVPDNSPFDLILVTVESSGLPPAWAAQLETGGRLVAPLAMRGLTRSIVFTRESSHWVGR
                     DPQLCGYVRPHGDEVEWEQTVALQGKHVSLRLESDAPEIDVDGLSSALSTTPVEYWSRV
                     NLSWDQSIAPLDLWLATCIDQFAILTGSTEAVARDVVARVSRTGAPTMVRGDNFGYRIA
                     RPIRDDLSLWEVGVRAHGPQATPLAHDFIKYVREWAVHHRGWPGPRILVHPSKTSLARY
                     SQGRVVNNRHARILISWFAP"
     misc_feature    8706..8708
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            10001..13003
                     /locus_tag="A37U_RS0140460"
     CDS             10001..13003
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155: Lantibiotic dehydratase domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /locus_tag="A37U_RS0140460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lantibiotic dehydratase"
                     /protein_id="WP_026198135.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1.3e-175,
                     bitscore: 579.2, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 4.6e-17,
                     bitscore: 56.6, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 2e-18, bitscore:
                     61.0, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPNSLFQHAGVGMLRAAAAGYIDKPTIWPDLTDPASCRSWIHSRW
                     GQQEWSKAIQRANPALARRLDTIQMRILLPEKQLPDKEVLGAAKSLVRYALRATGRHTP
                     FGLFAGVGLATITESTTHLHWGERDRVVARVDSDWLSTVIDELERDPEFLPLLKVVVNN
                     LTVRRGLRIDVPYGPHRVSLRCTKPVDAVLETAADPILFADLAMELGDTFPVPEEQITT
                     LLTDLVMQGVLITNLRAPSTEVDPLSYLVDALDYHSIGEIPFVTGIHTGLRNVRDRIAE
                     HNSQSGDADRAYLQAVNAMRALPVQATAKMSVDLRLDAHAQLPRHLARDLEAGASALAR
                     LSRQPTGHPVWRDWYMRFVERYGTNLPVPLLDAVNPDSGLGYPAGYPGSVLPAPEEAVS
                     ERDVKLLAYAWHTIATGDELVLDDTLIDQLRVGDPHTPLRVPPHVEIGATIHASSPLGI
                     DDGDYLCSVAPGRAAGTFTARLADLTDNGLDRLYASLPTCTRGASPVQLSCPPAYAHAQ
                     NISRTPQFLPHVLSLGEHRAPATAAVIPLDDLAVLATGDGGLCLLSRSRQKVLEPQAFN
                     ALAFDKQYAALARFLVHLPRAGHTVWHRFDWGPIAGTVPFLPRVRYGRTILSHARWRLS
                     RSNIPLENPRLGLERWRIRYACPKRVELTDHDRTLRLDLDDPAHRQLLLDHLDRHGEAV
                     LTEIVEHWSRRSDWIGHAHEVVFPLITTQDPVPTPTGVTRPAVTISRGDLPGRGDWLYA
                     KLYTHPDRIDELITVLDDELGDESWWVVRYRSNSELDHLRLRIATNTPGHRTDVFTGLG
                     QWAQRLRAKRDINRLVLDTYQPETGRYWHMPTAETVFTADTTVTALHLRTPGQLPRDGL
                     VALNMIGIAVGMLGSHAEAMRWFINRPAHPGPPVDRALLAQVTALVATSPPEDTPGWSP
                     ELDTAWRARADALATYRNHIPDSGRDAVIESLLHMHHNRLVGIDRDREHSARKLARHAA
                     LTWQHRTGATT"
     aSDomain        10475..10720
                     /aSDomain="Lanthipeptide_LanB_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing LanB protein in a class I
                     lanthipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_A37U_RS0140460_Lanthipeptide_LanB_RRE
                     .1"
                     /evalue="4.80E-18"
                     /identifier="RREFam001.1"
                     /label="A37U_RS0140460"
                     /locus_tag="A37U_RS0140460"
                     /protein_end="240"
                     /protein_start="158"
                     /score="56.6"
                     /tool="antismash"
                     /translation="VVVNNLTVRRGLRIDVPYGPHRVSLRCTKPVDAVLETAADPILFA
                     DLAMELGDTFPVPEEQITTLLTDLVMQGVLITNLRAP"
     misc_feature    12650..12652
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            13000..14121
                     /locus_tag="A37U_RS0140465"
     CDS             13000..14121
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140: Lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /locus_tag="A37U_RS0140465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lanthionine synthetase C family protein"
                     /protein_id="WP_063712903.1"
                     /sec_met_domain="LANC_like (E-value: 2.6e-41, bitscore:
                     135.6, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTSIGQETVRGDDLGSGAAGTALLRVQQAHAGTADRQELQRAIA
                     AMTGRGVAGHPDTASLYQGVPAVAFVLSTAGYVPVELEHQLDSITWHRIAQAHARIDRC
                     EHAGLGEYDLISGLTGLAVPLLALGHHDVLRDLLSYLIRLTEPIRDRPGWWYADPPPDG
                     DPEQFRGGHANFGLAHGINGPLTLLAIALREGITVPGHAEAIQRILSYLDRWELGTEHR
                     WWPASINAHEHETGRTRQRGPQRPSWCYGTPGQVRAQQLAALALGDTTRQQHVENIVYG
                     CVTDEWQLAQIIDESLCHGSAGLLHTLGRIAADSGDARLVGALTDLRERFRSSLSPEHV
                     GHDGFLEGNSGIQLIDHIPPAGAVRWDACLLLG"
     misc_feature    14107..14109
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            14170..15384
                     /gene="fxlM"
                     /locus_tag="A37U_RS47775"
     CDS             14170..15384
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="fxlM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Methyltransf_3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR04364.1"
                     /locus_tag="A37U_RS47775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methyltransferase, FxLD system"
                     /protein_id="WP_020502368.1"
                     /sec_met_domain="PCMT (E-value: 2.7e-47, bitscore: 155.3,
                     seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="Methyltransf_3 (E-value: 1.2e-06,
                     bitscore: 22.1, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTTSSPDELRNRLVDEVRNNGYATNPRVEYVMRTVARHAFVPEA
                     ELTEAYDDNTVVTKRDTDGKSLSCASEPSIVAMMLDQLDPQPGQRILEIGAGTGYNAAL
                     LAELVGPDGEVTTVDVDDEVASQARRNLDDQGYERVRVVTRDGVLGVPEHAKYDAIILT
                     VGAWDIPATWREQLVVGGRIVLPLRFRGTTRSILFVRPSSEDRLESHGMRLCGFVPLTS
                     QYGDREESIDVDGHVQIGWDCDQPIEASVLSGVLDLEGVTEWSTAEVGPFDPFDGIWLH
                     TAMHPGGCRISADENAVSTGLCKPIIRYRTPALVDSDSVAYLVAQPCEGSGDGDRRSRL
                     GATGHGPRARELVQAMLHAIAEWDTDRGRVPIATILQSGVRSESAADVLIRKTECRMML
                     RFEKS"
     misc_feature    14171..14185
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.49"
                     /tool="antismash"
     gene            15541..15873
                     /locus_tag="A37U_RS0140475"
     CDS             15541..15873
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A37U_RS0140475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157183354.1"
                     /transl_table=11
                     /translation="MAPIRRSTSNGLEIVGVTVEFLLEEAVEALMFRYRHLNLSRMQRS
                     PRLTVAEVRAALRNTAKHPRHLYMYDELGSEPPGGRLHLLQDWCVSELHRLRVFTPQRE
                     SAGAAQ"
     misc_feature    complement(15568..15582)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.54"
                     /tool="antismash"
     misc_feature    15604..15606
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(15895..15909)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     gene            16152..16496
                     /locus_tag="A37U_RS0140480"
     CDS             16152..16496
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152936.1"
                     /locus_tag="A37U_RS0140480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_020502370.1"
                     /transl_table=11
                     /translation="MVENGSTAGYPEDGPGSTGVDKAAIRARIDEECGLDSLSWRRAEP
                     EGEHIEDALEIAEATLDEVTYLLVRRPATIDDSRTEAQRLPLVFTPEEREAFLGGVDDG
                     DFGEIIPPAH"
     gene            complement(16894..17079)
                     /locus_tag="A37U_RS0140485"
     CDS             complement(16894..17079)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502372.1"
                     /locus_tag="A37U_RS0140485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_026198138.1"
                     /transl_table=11
                     /translation="MITNWRKSSRSDNANQCVELGWTGEIAGARDTAEDQLAAEARPVI
                     TASRRAIAAFADALRR"
     gene            complement(17235..17429)
                     /locus_tag="A37U_RS47780"
     CDS             complement(17235..17429)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502371.1"
                     /locus_tag="A37U_RS47780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_020502372.1"
                     /transl_table=11
                     /translation="MSQDITNWRKSGRSTNANQCVELGWTDEIAGVRDTAEDQLATEAR
                     PVIKASHQAIAAFADALRR"
     gene            complement(17426..>18187)
                     /locus_tag="A37U_RS0140495"
     CDS             complement(17426..>18187)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040183.3"
                     /locus_tag="A37U_RS0140495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5753 domain-containing protein"
                     /protein_id="WP_020502373.1"
                     /transl_table=11
                     /translation="AKQIGVKQPTIARFETSDSLPDQAHLQVLLELYGRKDQFTKLRQV
                     RDVAAARKRDQSMPIGTLSDIELLIGLEHFSSKIEVYETMVVTGLLQTRDYARAIIEKF
                     AVQESGVIVEDALDLRLARQAVLDRGDDELPPDLTMYVDESTLRLEIGGKKVLAGQLEH
                     LLKLEKRDNITIRVIPADVVYHAARDKSFTLLTFDDDWKMAYAEDFFSTHYHDSVKAIG
                     RASSLLTGLHGLALPASESRGLISDIREELT"
ORIGIN
        1 gcgttgaaga tctcacaaac agcggtatga cggttatcaa cgaccagtcc tacggtcctg
       61 atgttttcat tcggtgttat ccctccggcg atccggctac cggtgccgag aacttcagcg
      121 acacaagcac ggtccagcgg atcaacagct atacgtccga attgtttagc tggctttcac
      181 gacgactcgt tcccgacacg gaacagatcg ctcttagtaa gacgtccgca gccttctaca
      241 catcgaccgg cgaacccgtg gttgcactcc ggatctattc tctgaatccg cacttttccg
      301 atacacacgc caaagagctg atcggccacc tcagcgaagg taagaggcag ttcgattcgc
      361 tttgcctgag tgtcgaagag tggaagggaa ctgaatgaac aaggactcgc gcaacgggcg
      421 agctgaactg ccggtccgct tcacagtgcc caacctttcc gtgcgggact gcgttgagct
      481 ggccgcgggg ttggccacgt acgctcaaat ccacggaata ccgcagatcg aggtgagcgg
      541 cccaccggtg atcggcgagc tggcccagga gttcggcttc aacgagctgc gtttcgtcga
      601 cgaccatcag cagcctggga tcaacattcc agcagtgccc ccatcatggc ggtgtggcca
      661 acggggactg cgctgcttca gcgagcagat cgccgacact actcgagcac tgtcacctag
      721 cgacgacgtc gcgctgtcgc gcttgaccgc cgggtccgtg atgcggttca gtcgacataa
      781 ccgtcttggt gacgcactcg ggatcgtgtc cggcctggag cactactgcc gcgacaacga
      841 tgtcgagtcc gtcgccgttt ccggttcgca tagtctcgct gaggtagttg aagcgttcgc
      901 gtgtgaccga gttgcgcacg tctcatcgtc ctctgaggcg atcgatggcg atgcggtgtt
      961 cgctcgatcg tcgtggagtg tcccgtggct gagccggttc aatctcgcag tcgctgacat
     1021 cttcggtgga cgtgcccgtc atcaaacacc accgttgcgg ctgcgcgcga tgcagccacc
     1081 tgaaagtcgg ggaagcctgg tttattgcca gttcgacgcg cgatccagca cgccggtacc
     1141 acgggacgcg gcccgccatg tgctgagcaa acttgggtcc catggcgaag tccgagtact
     1201 cggcggcccc gacacgcaag catacctcgg tgaggacatc acgtacgttc gagcagacct
     1261 caacgagatg atcgcatgcc tgctgtcgtg ccgtctgttt gtcgggtgcg atagcggcgt
     1321 tgggcacctc gcgggatgcc tcggcgtacc cggtctgatc gtcagcaccg acgacttcga
     1381 gacaacgtgg tcgttctttc gtggctattc caacctcaat gtgatcccca tggcagcggc
     1441 agaattgttc ctgtcatgaa gcccccaacg gaacaagaag ttcttagagc agttggccgc
     1501 tgcctaggta cggaaaccgc gaccgcgagc cgaatctcga tgggccgcag tcacttcgtt
     1561 ttcacggtag cgcttgcttc cggtgagtcc gtgatcgcgc ggattacacg ccatgaccgc
     1621 gctgaatgtt tcaccgggtt cgcgtactgg cgatgcaagc tcgccaaggt gggcgtacct
     1681 gtccctcgcg tgctggccat ggatttgacc gagacagtcg tgccttggcc ggtcatggtc
     1741 cttgaacggg gtgccggtga tgatctctgc aatgtctacg ctggcctcag tgatgtggaa
     1801 aagcaagcag tcgctgacga tctgctcgac attcagcacc gcgtgagcac gctcccgccc
     1861 ggacgggggt acggcggtgt ggtcggctat cacgacaagc gctgtcacgg ccactggtcg
     1921 ggcgtgctcc gggagtacgt cgccggcgcc agcagtaacc tgtccgggaa aaaacgacag
     1981 aggccagcaa ttcgtgccat cgaaaccgcg ctcagcgagt tgcagcccga gttcgatcgc
     2041 atcgagccct tgccgtactt gatcgacgcg acgcaccaga acgtgttgat acacaaggga
     2101 cgagtaacgg cgattatcga tctcgacgag ctcgggttcg gcgacgccct gtaccctctc
     2161 ggtaccgccc gcacggggtt gctcgcccgc ggaccggagg cggactgtat cgaacgggtc
     2221 gccgcacccc tttgtgccac tcccctgcgt cgtcggattt tcgccttgta ctcagcgatc
     2281 gcgagcctga agatgctcgc cccgagcccg ctcggcgtca ccggcccacc ggcagctgat
     2341 agcccgatct ccgtgcggct gcatgctctg ctcgatgaat tcgccgccgc ggcgaacgag
     2401 gagtaacgct atggccggga tcgtgtacgt gagtatcgac gacttcagcc gggaggggtt
     2461 caccaacggc ttgtttctcc aggttcgcga gttactgcga cgggctacgg ctcgagggca
     2521 acaggcgagt ttggcttgta ttgctccgcg tatggcatcc ggagttgagc gcagaacccg
     2581 gactgtatct ggatgcacta tccacgagac gttcgttgca ccgccgggga aagcctcggc
     2641 gtatcgggct gcgtttcgag agctactgga tgaactcgcc ccggatgtga tcttgctaaa
     2701 cacctgcgca attcagctca ctcaggcaca tcacgtcgtg ctccgggaga gtttagccag
     2761 cgggaagcgc gtggtcgtgc tcgtgaccga ccagctgtac ccaacgagcg attcgcatcc
     2821 agacgacgaa atcctgcggt actacgacct gctgcgaaga accagcgtgc atgcggtcag
     2881 tcgaaccatg gctgaagcgt tccaaaagca gacgggagtg ccagccgctg tcttgcccaa
     2941 cctcctcccc gagccacaga ccgcccccac aacatcggac gagacggggc gacatctcac
     3001 cttgattaat caccatccga tcaaagggcg acgtgtctgg gatgccctca ttcggcagcg
     3061 gccacacgac gcctatctgg tcgtcgaaac ctggcccgat gttccgccat acacaccgcc
     3121 atcgccgcag gtcgagatag ctggctttgc cgaagacccc agcacgatct acaaccgcac
     3181 gaggattctg ctggtgccat ctctgggacc agaggggttg tcccgcgtga cactcgaaac
     3241 gatggaatcc ggggttcccg tggtggccca tcacatcggc agcctttccg agctggaaag
     3301 cgccgcagtt ctggtcactc caccaccgat cgtcgggtac gaactcgacg gcggcgtact
     3361 gtatccggtg gtctcaccat cggatatccg gcgagcggca ctcgatttca acgagaggat
     3421 cgacgcctta gatcacgatg cctcgtttcg ggagcggtgt gtgaacagcg gtcgagctct
     3481 tgtccggaac taccgggccc tcagcgaggc tgccgctgac cagttgcttg acacatggtt
     3541 cgccggaacg tgaagcaggt cacgctcgga gcgttcgccg tcaaggctcg tgttctgcga
     3601 ggtaggagtc taaccagcac acatggcacg cgagctcagc cggagcacta cactggtcgc
     3661 cgtaccaggt acccctctca aggagatacg cgacagtgac ggccatcgtc gacgaactca
     3721 agtggcgtgg gctgatcgcc cacaccactg acgaggacgc attacacaag gcgcttacgg
     3781 atggtcccgt catcttctat tgcggcttcg atccgaccgc ggccagcttg catgtcgggc
     3841 acttagtcca ggtcctgact atgcgtcggc tccagcaggc cggccaccga cctctggcac
     3901 tggtcggggg tgcgactggc catatcggtg acccacgccc gaccgccgag cgcacgctga
     3961 atgattccga gaccatcgca gagtgggcta tccggcttcg agcacaaatc gagccctacc
     4021 tcagtttcga gggcgagaac gccgcgatga tggtcaacaa cctggactgg acggcgaaca
     4081 tgtccgtcat cgagttccta cgagatgtcg gcaagcattt ccgggtgaac aagatgctca
     4141 ccaaggactc ggtcgcgcag cgcctcgaat ccgaccaggg catcagctac acggaattca
     4201 gctaccagtt gctgcaggga atggacttcc tcgagctgta ccggcggtat gggtgcacgt
     4261 tgcagacggg tggaagtgac cagtggggca atctgacagc tggtttggac ctgatccatc
     4321 ggctggagac ggatgccgag gttcacgcgt tcggaacacc gctgatgacc aaggctgatg
     4381 gaagcaagtt cggtaagtcc gagaccggga cgctctggct cgatcccgag atgaccacgc
     4441 cgtacgcgtt ttaccagttc tggttgaaca ccgatgatcg ggacattggc cgctacctgc
     4501 gggtgctgtc gttcaagtcg cgcgaggagc tactggagct ggagaaggag accgaggaga
     4561 aaccgcaggc gcgcgccgcg cagcacgctc tggctgagga actcacgacg ctggtgcacg
     4621 gcgctgacca gtgcgatgcc gcgatcgcgg catcgcaggc gctgttcggt caaggcgagc
     4681 tcggcgacct cgacgaaacg accctgacgg ctgcggtagc cgaactcccg aaggcgaggg
     4741 tgagcgaacc cggtcaggtc gtcgacctgt tcgcggaggt cggccttgtg ggcagtaagt
     4801 cggcagcccg ccgcaccgtg aaggaaggcg gtgcctacgt caacaatgtg aaagtcaccg
     4861 ccgaagacgc cgcggttacc gtcgatcagc tgctgcatgg gcggtggctg atcttgcgca
     4921 agggcaagaa gaacctggca gcggtcgagt tcaccggagc ctgatatcag ctcgatgcgg
     4981 tcggttgaga ctgctcgaaa gcgcacccac cgctatgaca caatccgtca acttcggttg
     5041 tcttgagcat gcgattcgat catgcctgcg tgcaccgcct acagatttga gcgaaatcgc
     5101 gtagcggcaa ggccaatgcg agctcgccgt cgttggcggg tttcgggctg aacactttcc
     5161 cacagtccgc atagatcgtg acgccagttt gttcgcagcg atgcacaccg cggtcgccaa
     5221 ggtgctgaat gaaccagtag gcaccctctg acgcgcgtgg tggacctacg agcaggggtt
     5281 cgcgcggatc cagcgagtcg gtatcgccgt agctttctag cgcgtgaatg gcttgtcgca
     5341 gcgcggcggt agtaatccgg atgcgctcgg ggctctgctc tcgactcgct cggttgtaga
     5401 gcatgtcttg gagcagacgt ccggcccgca tgagttcacg cttgtgcgct tcacgtcgat
     5461 gctcgggtgg ttgattcact acgcaagccc tccttccggg caacttcaac gggcgatctc
     5521 ggcggcgctg gaagttgaac gagccggcca cattgcgcgt gggctggagt agtccacccg
     5581 cctgacgacg cggccatgtt gcataacctt tagactgtat agtatacagc tatagtgcta
     5641 ttcacctgca gggtcatgca tagcagtgtc gtcttgttgc gtgctcgagc tctcgaggct
     5701 ggcaatgcgg tgctgcggaa gtctcaagta cctgcgcgag ttcagaagaa ggcgaacctg
     5761 atgtcagatc gcgttcatag ctccggcacc cacacggatc actcccatgc tcctctgcga
     5821 ctcgtgggtg ggttactcat cgttgtgctc atcatcgctg tgggatacat gttcgcggcg
     5881 gtgacacagc aatgagcaag tacccggtat gcgccatcgt cgacggcatt ggctccgtca
     5941 gcgcgactcg ctcgactcga ttgcacggcg gaagcatcgt tgatcctggt tcgaatggca
     6001 gtacggacgt cgggacggtg gtccgccttg gcttggctgc ggactggtcg ctcatgctgg
     6061 tggcctcgat cgctggggcg gcatcgtgat cgccgcccca gatgttcggg cgagatgtcg
     6121 ccgcacggtt cgggcggcgg tgctgctgtt cgccgtactc gtagtgttga tcctcgtggc
     6181 gttggctgca ctggtgttac tgctccggac ggacgtgatg gcgtatgcct tctccggatg
     6241 aattcaggcg ggtgacagcg gagcagtacc tcttcattcg tagttctttc cggcttgacc
     6301 agaacccgga tgaggtcgcg aggatcctgc agctgtccgt cgagacggtg ctgcggcacc
     6361 agccgcgagc gaatgttcca cggcatgcac ggggcaagca tctaaccgac gagctcaggt
     6421 cagagatccg gacagcatgg tgcgcggggc tgacattgaa gcagatcacg cgggagttcg
     6481 agatcggtga tcaaaccgcg ttgcggtacc ggccgtcgga gctggcgccg aggcaaagga
     6541 tctccgaaat acaggagagc atcatcgtgc aaggctggcg ggacaaggtg aatccgagcg
     6601 acattgccga ccgtgccggg gttgggctca caacggtgta caagtaccgg ctgcggtgat
     6661 ggttgcgatg cgaacatcga atcgattggc gcatgcccgc ggatcacagg cgtggtgcgg
     6721 cttgttgcgg ggcatgctgg agagaccggc agtggtcggc gtaagcctcg gtcgaaggca
     6781 gcagctcgat tcgaggctgg tcaacctggc agatcttccg cacaggttcg tcacgggtac
     6841 ctggtcgtga tgcggcgcct gacagttgtt gcggtgcccg ctcacggccg tggccacata
     6901 tccgtgttct gccttttcga ggagcgttac agcaagtgct cacgtcagtt gatgtccccg
     6961 tctcggaggc tgttcgccgt gccattcaga aggaagccgc cagggagtcg tctccgcgta
     7021 ccatcgctgg gaagctgggg atttcggtcg cgcaggtgat gcagcattat ccgcatcccg
     7081 agccgcagac gtttgtgatc ccgccgcaac cgcagcgggg ccctcgggac ccgctgatcg
     7141 cggagcaggc acatgcaatg cggattgcgt ggctcgctgg atggacttcg aacgagattg
     7201 ccacacattt ccaggtttcg aagcagacgg tgttgaagta ccaaccatgg gagatcctga
     7261 agctacggga gcttgcctct acgggtacag ctccggattg gcatgagctt tccggcgaag
     7321 tgcagctggt gatcctgtgg gccgagagtt tggggtggcg ggtcaaggtg attgcgtcgc
     7381 ggctcaagct tcccgccgtg gccgtgcgga agtatctggc ggccgactat gaccggtgat
     7441 cgttctgaac tgacggcgcg gttatatcta caaattcggc gcgagttgcg actgggcgtt
     7501 gatccggacg agcttgccgc aaggatgcta cttcccgcgg gaatcgtgtg ggaacagatc
     7561 ccgcgccata agcggatagc gaaggcgttg ccgcgagtta cggaggagaa acgcacggaa
     7621 atccgacgcg gatgggacag gggactgagt ggcgagcagg ttgcgaggtt agtcagtgtt
     7681 tcaccattta ccgcgcgccg gtacggatcg cggcccaagc agcgatcgac gcagtccgag
     7741 ctcgaaccgc catcaccagc gcgggaagac gacggaactg atgtgcaaga caagacggaa
     7801 gccgaatttc gggtgttgaa tcggcagcgg gtgcaagcag tgcgatcagg gcgaaaagca
     7861 ggggtttccc tcgacgtaat ggcctctgag ctgagttgcc ccgtgcagca gataagtcgg
     7921 ctctgcctgg attctgtgca accgagccaa cagctctcgg aaagggatca gtcggtaatc
     7981 aatcgaatgt ggaccctgcg gatccggcag atgccggcaa agacagaaaa gggcctcggg
     8041 cctctagtgg tgcgctggac gcaacgcccc acagcgtccg gaaccaacag tcatgacagt
     8101 aggtagtcgt tgctgaggtt gcgaatcacc cgataagagt atgcatagct cttgtcaacg
     8161 cataatagtt tgactgtata gtatacagcc aacgaggtat tcgcatcgag gcggaggggt
     8221 cgcatgagct cgcaactctc cccgtgatgc gcacacagac tcctcgccgc gaccatggct
     8281 gacgcgttgg tcccctcact ggggatgacc aacggatttg ttcagtgatg gaagccggga
     8341 aacgttccca cacccggtga tgagatcacc ttgttgatct ggtgacgatc tcaccgccgg
     8401 gaatgccgcg ctgccctcgg gaagcgtggc ttcccagcga aaggcagcgc gaaatgcact
     8461 tcgaaacccg tgatgccgcc agaggcgacg gaccagatcc tgccgatcca gatcgtgtcg
     8521 acgagttccg ccatgcgatg gtcgacgacc tgcttcaaga ccgaaccttg tattcgacgc
     8581 gggtcgcgcg cgccatgcgg ggtgttcccc gccatctgtt cgcttccggc gtgccgctcg
     8641 aggaggcgta ctcggacatc acgctggtca ccgatcgcga cgaggatggc cacgcgcttc
     8701 acgtattatc tgctcccggt gtgcaggcca cgatgctgca gcaagccgat gttcggcccg
     8761 ggcaacgcgt cctggagatc gggtccggtg gctacaacgc tgcgttgatt gccgacctga
     8821 ctggacctga cggtgaggtc acgacggtcg atacctgccc cgagcaagcg gctcgtaccc
     8881 gccgtgatct tgatacggcc ggatatccgc atgttcaagc cgtccacgcc aaggtcgagc
     8941 agggggttcc cgacaactcg ccgttcgacc tgatcctggt caccgtcgaa tcttcaggtc
     9001 tccctccggc gtgggcagcg cagctggaga ccggggggcg actcgttgcg ccgctggcca
     9061 tgcgcggctt gacgcgctca atcgtgttta cccgcgaatc cagtcactgg gtcggacgcg
     9121 atccgcaact gtgcgggtat gttcgtcccc acggcgacga agtcgaatgg gagcagacgg
     9181 ttgccctgca aggcaaacac gtcagcctcc gccttgagag tgacgcccct gagatcgatg
     9241 ttgacgggct aagcagtgcc ctgagcacga ctccggtgga gtactggagc agggtcaatc
     9301 tgtcatggga ccagtcgatt gcgccgctgg atctgtggct ggctacgtgc atcgaccagt
     9361 tcgcgattct gactggaagc accgaagccg tcgctcggga cgtggtggcg cgcgtcagtc
     9421 gaaccggcgc gcccacgatg gtgcgtgggg acaacttcgg gtaccgaatc gcccggccga
     9481 ttcgcgatga cctgagtttg tgggaagtcg gtgttcgcgc tcacggccct caggccacgc
     9541 cgcttgcaca tgacttcatt aagtacgtgc gcgagtgggc ggtgcaccac cgcggatggc
     9601 caggaccgcg cattctcgta cacccttcca agacgtcgct cgctcgctat tcgcagggcc
     9661 gcgtcgtcaa caaccggcat gcccggatcc tgatctcctg gttcgctccc tgacgggaag
     9721 gccaggtgtc ccaaggaaaa accgattgat tccagaggag gaacactcct atgaccgctc
     9781 cctcgatacc gggcgacctg ctcgatgaat tcgacctcga cgtgaacatc ctgaccgacc
     9841 cgagtcaggt cgcagcaccg tgcaagaccg atgacggttg caaaccaacg tgcgcttccg
     9901 cctgcactag caacgcctga ccacgatcgt cgcggcagtc tcggagcccc acgttccgaa
     9961 ggcggaacgg ggctccgagg cggcctgttc tggagaaacg atgcccaaca gcttgttcca
    10021 gcacgcgggc gtcggtatgc ttcgcgccgc cgctgccggg tatatcgaca agcccacgat
    10081 ctggccggat ctgacggacc ctgcatcgtg ccgaagctgg atccacagcc gctgggggca
    10141 acaggagtgg tcgaaggcga tccagcgagc gaatcccgct ctggcccgac gtctcgacac
    10201 catacagatg agaattcttc ttcctgaaaa acaactgcct gacaaggaag tacttggcgc
    10261 cgccaagagt ctcgttcgct atgcgctgcg cgcgactgga cggcacaccc cgttcgggtt
    10321 gttcgcagga gttggtctcg cgacgatcac tgaatccacc acgcatctgc actggggtga
    10381 gcgggatcgt gttgtcgcgc gggtggattc cgattggttg agcaccgtca tcgatgagct
    10441 cgagcgtgac ccggagtttt tgccgttgct gaaggtcgtc gtcaacaacc tcacggttcg
    10501 gcgtgggctt cggattgacg ttccctacgg tcctcatcga gtcagcttgc gctgtacgaa
    10561 gcccgtggac gcggttctgg agaccgccgc cgatccgatt ctgttcgccg acctcgcgat
    10621 ggagttgggc gacacgttcc cggttcccga ggaacagatc actaccttgc tgaccgacct
    10681 ggtcatgcag ggcgtgctca ttacgaacct gcgcgccccg agcaccgaag tagatccgct
    10741 cagctatctc gtcgacgcgc tcgactacca cagcatcggt gaaatccctt tcgtgactgg
    10801 tatccatacc ggcttgcgga acgttcgcga ccggattgcc gaacacaaca gccagtccgg
    10861 cgatgccgat cgtgcttacc tccaggcggt caacgcgatg cgcgcgctgc ccgtgcaggc
    10921 cacggccaag atgagtgtgg acctgcgttt ggacgcgcac gctcagcttc cccgacatct
    10981 cgcccgagat ctcgaggccg gcgcgtccgc gctggctcga ctttccaggc aacctaccgg
    11041 ccaccctgtt tggcgcgact ggtacatgcg gtttgtggaa cgctacggaa ccaacctacc
    11101 ggtaccgttg ctggacgccg tcaatcctga cagcgggctc ggataccccg ctggttatcc
    11161 cggaagcgtc cttcctgccc ccgaggaggc agtgagcgag cgcgacgtga agctgcttgc
    11221 ctatgcctgg cataccatcg ctactggcga cgagctggtc cttgatgaca ccctgatcga
    11281 ccaactccgt gtgggcgatc cgcatacgcc acttcgcgta ccgccgcatg tcgaaatcgg
    11341 tgccacgatc catgcctcca gcccgttggg catcgacgac ggcgactacc tgtgtagcgt
    11401 ggcgccggga cgcgcggcgg ggacattcac cgcgcggctt gccgatctca cagacaatgg
    11461 cctcgaccgg ctgtacgcct cgctgccgac ctgtacgcgc ggtgcatcac ccgtgcagct
    11521 gtcctgtcca ccggcttatg cgcacgcaca gaacatcagc cgtaccccgc aattcctccc
    11581 tcatgtcctc tccctcggtg aacaccgtgc ccctgccacc gcggcagtga ttccgctcga
    11641 tgatctcgcg gtcctggcca ccggcgatgg aggtttgtgt ttgttgagcc gaagtcgcca
    11701 gaaagtcctt gagccgcaag cgttcaatgc gctcgcgttc gacaagcagt acgcggcgct
    11761 ggcccggttc ttggtacacc ttccccgggc cggtcacacg gtgtggcatc ggtttgattg
    11821 gggaccgata gcgggcacgg tgccgttcct gccgcgcgtg cgctatggcc gaacgatcct
    11881 ttcgcacgcc cgttggcgtc tttcccgtag caacattcct ctcgagaatc cgcgtctggg
    11941 attggaacga tggcggatcc ggtacgcctg ccccaagcgg gtcgagctga ccgatcatga
    12001 ccggacactg cgcttggatc tcgacgatcc ggcgcaccgg cagctcttgc tcgaccacct
    12061 cgatcggcac ggcgaagccg tgctgaccga gatcgtggag cactggtcgc gccgcagcga
    12121 ctggatcggg catgctcacg aagtcgtttt tcccctgatc acgacacaag atcccgtccc
    12181 cactcctacc ggcgttactc gtccggctgt gaccatcagc cgcggggacc tgcccggtcg
    12241 cggcgattgg ttgtacgcga agctctacac ccaccccgac cggatagacg agctgatcac
    12301 cgtcctggac gatgagctcg gcgacgaatc gtggtgggtc gttcgctacc gcagtaactc
    12361 cgagctcgat catctccggc tgcgcatcgc gaccaacacg cccggacacc gcaccgacgt
    12421 cttcactggc ctcggccagt gggcacaacg cttgcgtgcc aaacgagaca tcaaccggct
    12481 ggtgctcgac acctaccagc ccgagaccgg ccgctactgg cacatgccga ccgctgagac
    12541 cgtgttcacc gccgacacca cggtcaccgc gctgcacctg cgaacaccgg gccagttgcc
    12601 gcgcgacggt ctagtcgcgc tgaacatgat cggtatcgcc gtcgggatgt taggcagcca
    12661 cgccgaggcg atgcgctggt tcatcaaccg acccgcccat cccggaccgc cagtcgaccg
    12721 cgccctcctg gcgcaagtca ccgccctggt cgcgacgagc ccaccagagg atacgcctgg
    12781 ctggagcccg gaactcgata ccgcgtggcg ggcccgcgcc gacgcgctgg ccacctaccg
    12841 gaaccacatt cccgacagcg gtcgggacgc ggtcatcgag tcgctgttgc acatgcacca
    12901 caaccgcctg gtgggtatcg accgcgaccg cgagcactcc gcccgtaaac tcgcgcgcca
    12961 cgccgccctc acctggcaac accgcaccgg agcgaccaca tgaccacctc aatcggacaa
    13021 gagaccgtgc gcggcgacga cctcggttcc ggggccgctg gaaccgccct tctgcgcgtc
    13081 cagcaagctc acgcgggaac cgcggatcgc caggaactcc aacgagcaat cgcggccatg
    13141 accgggcgtg gtgtggccgg gcacccggat acggcttccc tctaccaggg tgtgcccgct
    13201 gtcgcgttcg tcttgagcac cgccggctac gtgcctgtcg agctcgagca ccagctcgac
    13261 agcatcacct ggcaccggat cgcgcaggcc catgctcgca tcgataggtg cgagcacgcc
    13321 ggtctggggg aatacgactt gatctccggc ctcactggtc ttgccgtccc gttgctggcg
    13381 ctcgggcatc atgatgtgct ccgtgacctg ttgtcctacc tgatacggct gaccgagccg
    13441 atccgcgatc gacctggatg gtggtatgcc gatcctccac cggacgggga ccccgaacag
    13501 tttcgcggcg gacacgccaa cttcggtctc gctcacggca ttaacggccc gctcacgcta
    13561 ctcgccatcg ctttgcgcga gggcattacc gtccccggcc atgccgaggc gatccagcgc
    13621 attctctcct acctcgaccg gtgggaactc ggcacggaac accgctggtg gcccgcaagc
    13681 atcaacgcgc acgagcacga gaccggccgt acacgccagc gcggtccaca gcgtccgtcg
    13741 tggtgctatg gcacacccgg gcaggttcga gcccaacagc tcgccgcgct cgcgctcggc
    13801 gacaccacgc gccagcagca tgtcgagaac atcgtctacg gctgcgtcac ggatgaatgg
    13861 caactcgccc agatcatcga tgagtcgctg tgccatggat cggcgggcct gttgcacacc
    13921 ctcggcagga tcgcggccga cagtggcgat gcgcggttgg tcggcgcact caccgacctg
    13981 cgcgagcgat tccgcagcag cctcagcccc gagcacgtcg gccacgacgg gttcctcgaa
    14041 ggaaacagcg gaattcaact catcgaccac attccacccg cgggcgcggt ccgatgggat
    14101 gcctgcttac tgctcggata gcacccccaa gcccgcagaa gtgtagctac gaacaggaag
    14161 gaactgccgg tgaataccac ttccagcccc gacgaactcc gcaaccgcct tgtcgacgag
    14221 gttcgaaaca acgggtacgc caccaatccg cgcgtcgaat acgtcatgcg caccgtggcc
    14281 cgccacgcgt tcgtgcctga ggcggagctg acggaagcgt atgacgacaa cacggtcgtc
    14341 acgaaacgcg acaccgatgg caaatcgctg tcgtgtgcgt ccgagccgtc gatcgtggcg
    14401 atgatgctcg atcagctcga cccacaacct gggcagcgaa ttctcgaaat cggagcgggg
    14461 actggctaca acgccgcctt gttggccgag cttgtcggcc cggacggcga ggtcaccacc
    14521 gtcgacgtcg atgacgaggt cgcgtcccag gcgcgccgca atctggacga tcaggggtac
    14581 gagcgtgtcc gcgtcgtcac gcgtgacggt gtcctcggtg tccccgaaca cgccaaatac
    14641 gatgcgatca tccttaccgt gggggcctgg gacattcccg cgacctggcg agaacagctt
    14701 gttgtcggcg ggcgaatagt gttgccactg cggttccgcg gaacaactcg ctcgatcctg
    14761 ttcgttcgcc cctccagcga ggacaggctc gaatcgcatg gcatgcgact gtgcgggttt
    14821 gtgccgctga ccagccagta cggtgatcgg gaagaatcca tcgacgtcga cgggcatgtt
    14881 cagatcgggt gggactgcga tcagccgatc gaggcgagtg ttctgtccgg cgttctggac
    14941 ctggaagggg tcaccgaatg gtcgaccgcg gaggtcgggc cgttcgaccc attcgatgga
    15001 atctggttgc acactgcgat gcacccaggc ggatgccgga tttccgccga tgagaacgcc
    15061 gtctcgactg gcctgtgtaa accgatcatt cgctaccgca cgccggctct ggtcgattcc
    15121 gacagcgtcg cctacctggt cgctcaaccc tgcgaaggct ccggcgatgg tgatcggcgc
    15181 tcgaggctcg gagcgactgg gcacgggccc agggcgcggg agctggtcca ggcgatgctg
    15241 cacgcgatcg ctgagtggga caccgatcgt gggcgagtgc cgatcgcgac gatcctgcag
    15301 tccggcgttc gcagcgagtc cgccgcggat gtgttgattc gcaagaccga gtgccgcatg
    15361 atgctgcgtt ttgagaagtc gtgaacacca tgaggctgct gaatctgcaa cgaatcctgt
    15421 ggccggtggc acgcctggat catcagcgca ccggcggacg agttcccgac gctccccggg
    15481 atgtgttgca ctcagcaggc gagtccgtct accggcagac gatcataggg aagtcctgac
    15541 atggcaccaa ttcgtcgtag caccagcaat ggactggaaa ttgttggggt gactgtcgag
    15601 ttcttacttg aagaagccgt cgaggcgctc atgttccgat accgtcacct gaatctgtcg
    15661 cgcatgcagc gttcaccgcg gcttaccgtc gctgaggttc gggcggcgct gcgcaatacg
    15721 gccaagcatc cgcggcacct ctacatgtat gacgaactgg gttcggaacc gccgggcggg
    15781 cggctgcacc tgcttcagga ctggtgcgtc agcgagctgc accggctacg cgttttcacg
    15841 ccacaaagag agtcggcggg cgctgcacag tagatcggga acagaagccg ttggatcgac
    15901 gaaggtgaac gtccggacca cgtcgtacca gtccgagacc ggccgtcggt gcatgcttcc
    15961 tcgcatgacc tccctccgat ttgccatacg aggccacgtc gccgctcctg gctcggccgg
    16021 aagatgacga cactcgaccg gcgctaccgg cgaggtgtcc tgccttgtgg atctcagagt
    16081 cggcagctgt gcaatgctgg ccatcggtac gaacatcggc cccggatcat gaaacgagac
    16141 ttggctacgc aatggtggag aacggcagca cagcaggata ccccgaggac ggtcctggct
    16201 cgaccggagt agacaaagcc gccatccggg cccgtatcga tgaggaatgc gggctcgaca
    16261 gcctcagctg gcgccgcgca gagcccgagg gggaacacat cgaggacgcc ttggagatcg
    16321 ccgaggcgac gctcgacgag gtgacctacc tcctggtgcg tcgacccgcg acgatcgacg
    16381 acagtcgcac cgaggcacag cggcttccgc tggtgtttac gcctgaagag cgggaagcct
    16441 tcttgggtgg ggtcgatgat ggtgacttcg gcgagatcat tccgcccgcg cactaagaat
    16501 tctggcttgg ctcaggcctc ttattgtagg tcaggttttc cagaggtcta tttatcgtag
    16561 gctccgctga gtagaccggc caaaattgca ctggtccgct cgggtgagga cggcacgttg
    16621 cagttgcgtc ctgccggggt tctcggcaac atgcgatgcg gacgtccacc gctctcgaat
    16681 cgctgatgag agcacgaata accgaggttc tgtctccacg agcaatggcg tggagacaga
    16741 acctcggcat gcacacagtc accggcacgg tccgtcatgg ggccggagtc ggccacacgc
    16801 ggcagtcgga ggaccgcgcc agagagggct gttcaacggc cgggcggtct agctcgcggg
    16861 acgcgagtcc atgccactgg agcgcagcgg ggattaccgg cgcagcgcat cggcgaacgc
    16921 ggcgatggcg cggcggctgg cggtgatgac cggccgggcc tcggcggcga gctggtcctc
    16981 ggcggtgtcg cgggcacccg cgatctcgcc agtccagccc aactcgacac actggttggc
    17041 gttgtcgctt cggctggact tgcgccagtt cgtgatcatc gcgctcagct ccgcgttgtc
    17101 ggcgaagtgg accgcacaca ttctcagggc ccaggagact ggcgcggtag ccgccggacg
    17161 ggtgcgaatg accgaggccc cgcacactgc aatggatgtg tgcggggcct cgggtctttg
    17221 actacgtgcc gcgattatcg gcgcagcgcg tcggcgaatg cggcgatcgc ctggtgactg
    17281 gccttgatga ccggccttgc ctctgtggcg agctggtctt cggcggtgtc gcggacaccc
    17341 gcgatctcgt cggtccaccc cagctcgacg cactggttgg cgttggtgct acggccggac
    17401 ttgcgccagt tcgtaatgtc ctggctcatg tcagctcctc tcgaatatcg ctgatgagac
    17461 cccgtgattc tgacgcaggg agggccaggc cgtgcaagcc ggttaggagt gagctcgcgc
    17521 gaccgattgc cttgacgctg tcgtggtagt gcgtcgagaa gaagtcctcg gcataagcca
    17581 tcttccagtc gtcatcgaag gtgagcagcg tgaacgactt gtcgcgtgcc gcgtgataca
    17641 ccacgtcggc cgggatcact cggatcgtga tgttgtctcg tttctcgagc ttgagcaaat
    17701 gttcaagctg cccagccaag accttcttgc caccgatctc caaccgcaag gtgctctcat
    17761 cgacgtacat cgtgaggtct ggcgggagct cgtcgtcgcc gcggtcaagg acggcctgcc
    17821 tggccaggcg aaggtcgagt gcgtcttcga cgatcacgcc ggactcttga acggcgaact
    17881 tctcgatgat cgcccgtgcg tagtcacgag tctggagcag gccggtcaca accatggtct
    17941 cgtagacttc gatcttgctg gagaagtgct ccagtccgat cagcagctcg atgtccgaca
    18001 acgtgccgat cggcatgctt tggtcacgtt ttcgcgccgc ggcaacgtcc ctgacctgcc
    18061 gcaacttggt gaactggtcc ttacggccgt agagctcgag cagtacttgc aggtgcgctt
    18121 gatcgggaag tgagtcgctg gtttcgaacc gcgcgatggt gggctgcttg acgccgatct
    18181 gtttggc
//
