LOCUS       NZ_KB905433             9184 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Sciscionella marina DSM 45152 A37UDRAFT_scaffold_57.58, whole genome
            shotgun sequence.
ACCESSION   NZ_KB905433
VERSION     NZ_KB905433.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Sciscionella marina DSM 45152
  ORGANISM  Sciscionella marina DSM 45152
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Sciscionella.
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K.,
            Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A.,
            Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Sciscionella marina DSM 45152
            collection_date                   :: SEP 2006
            lat_lon                           :: Missing
            depth                             :: 516 m
            alt_elev                          :: Missing
            country                           :: China
            num_replicons                     :: Missing
            ref_biomaterial                   :: DSM 45152
            biotic_relationship               :: Free living
            rel_to_oxygen                     :: Aerobe
            isol_growth_condt                 :: 19196758
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Sand; Sediment; Marine; Aquatic
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi11892
            Current Finishing Status          :: Level 2: High-Quality Draft
            Type Strain                       :: Yes
            Funding Program                   :: DOE-CSP 2012
            Isolation Site :: Marine sand sediment at a depth of about 500 m,
            the northern South China Sea
            Cell Shape                        :: Rod-shaped
            Temperature Range                 :: Mesophile
            Temperature Optimum               :: 28 C
            pH                                :: 7
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000379465.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 01:37:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,209
            CDSs (total)                      :: 8,146
            Genes (coding)                    :: 7,961
            CDSs (with protein)               :: 7,961
            Genes (RNA)                       :: 63
            rRNAs                             :: 4, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 1 of 185
            Pseudo Genes (frameshifted)       :: 52 of 185
            Pseudo Genes (incomplete)         :: 155 of 185
            Pseudo Genes (internal stop)      :: 15 of 185
            Pseudo Genes (multiple problems)  :: 34 of 185
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:59:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9184
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB905433.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405877
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Nikos  Kyrpides (nckyrpides@lbl.gov)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-PGF and JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..9184
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /tool="antismash"
     gene            complement(<1..670)
                     /locus_tag="A37U_RS51175"
     CDS             complement(<1..670)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /locus_tag="A37U_RS51175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="IS3 family transposase"
                     /protein_id="WP_020496718.1"
                     /transl_table=11
                     /translation="MKELVAAGARIRVPVAVACRVLKLHRQHYYAWLARPVTDTELAEA
                     YLANAIYDAHHEDPEFGYRLLRDEITAAGHQVSDRTVWKICSTNQWWCVFGKKRRGRKS
                     RPSAPAHDDLVRREFTAHAPNRLWLTDISEHPSNEGKVYLCAVKDVWSNRIVGYSIADR
                     XSAQLALDALESAVARRGGTVAECTLHSDRGGQFRSRKPQQSLWRHQIRGSMGQVGSAG
                     D"
     source          1..9184
                     /collection_date="2006-09"
                     /country="China"
                     /culture_collection="DSM:45152"
                     /db_xref="taxon:1123244"
                     /isolation_source="Marine sand sediment at a depth of about
                     500 m, the northern South China Sea"
                     /mol_type="genomic DNA"
                     /organism="Sciscionella marina DSM 45152"
                     /strain="DSM 45152"
                     /submitter_seqid="A37UDRAFT_scaffold_57.58"
                     /type_material="type strain of Sciscionella marina"
     cand_cluster    1..9184
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9184
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2925:7265](+)"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2926..7265
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     misc_feature    complement(999..1013)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.07"
                     /tool="antismash"
     gene            1095..1706
                     /locus_tag="A37U_RS47060"
     CDS             1095..1706
                     /NRPS_PKS="Domain: PKS_KR (15-133). E-value: 9.7e-08.
                     Score: 24.1. Matches aSDomain:
                     nrpspksdomains_A37U_RS47060_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /locus_tag="A37U_RS47060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_020501484.1"
                     /transl_table=11
                     /translation="MLWDRSERLLQEACNSMVAGESVRTAVVDVTDFDAVVEAASDAER
                     VLGRVDVLVNSAGVTCPPVPIGQYRTEDWQRILDGNLSGVFFCCRAVLPGMIAQQYGRV
                     VNVASMAGKEGNPQETAYSAAKAGVIGLTKALGKEVAASGVLVNAVAPGIFATPMRTSA
                     ASRELVGKLLDRTPPWPWPARQASRTATLICAVNRTVHRW"
     misc_feature    complement(1122..1138)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.07"
                     /tool="antismash"
     aSDomain        1140..1493
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A37U_RS47060_PKS_KR.1"
                     /evalue="9.70E-08"
                     /label="A37U_RS47060_PKS_KR.1"
                     /locus_tag="A37U_RS47060"
                     /protein_end="133"
                     /protein_start="15"
                     /score="24.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="SMVAGESVRTAVVDVTDFDAVVEAASDAERVLGRVDVLVNSAGVT
                     CPPVPIGQYRTEDWQRILDGNLSGVFFCCRAVLPGMIAQQYGRVVNVASMAGKEGNPQE
                     TAYSAAKAGVIGLT"
     gene            complement(2323..2493)
                     /locus_tag="A37U_RS52290"
     CDS             complement(2323..2493)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A37U_RS52290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157183251.1"
                     /transl_table=11
                     /translation="MSAPIAQRQDELDIFDLEVRTVPADGEIAKSRMKPTATPGTCNSE
                     CGLTRPGFPGC"
     misc_feature    2561..2577
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.5"
                     /tool="antismash"
     gene            2926..5976
                     /locus_tag="A37U_RS0135860"
     CDS             2926..5976
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155: Lantibiotic dehydratase domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /locus_tag="A37U_RS0135860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lantibiotic dehydratase"
                     /protein_id="WP_020501485.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1e-180, bitscore:
                     591.0, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 1.2e-16,
                     bitscore: 50.5, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 3.4e-37, bitscore:
                     117.6, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVLPHHRAYALAAERDPGQGNERDELIRILRTVVADPLVREALE
                     VSSDSWATGLRRLEEGEHVRTRQLRRATYAACRYLLRMTTRPTPFGLMAGVAVGSFDDE
                     PLVELGKAHRKGVRPDGEWLTALLTEWESRTEVVRCLRVCANDLYFERAGRFVLPYVPL
                     RDPTGQPAEEKSVRATPPARTAMTVARAPIAFTELVDRLVEQYPQAAGTDRVEQLLMGL
                     IETGLLCTELSPPPHCTDPITYVRATLERTLAPETAGLGNIAARFDEYARAPLGSGRQA
                     WGAATSEAHELHSGAGSPIQVDLRMDARITLPHTVAREVESAASVLLRLSPASGSPPHL
                     ANYYDEFLDRYGSGNAVPVKEVLDPERGIGPPAGYRLPANSRLGEPTQERDGPRNRKLA
                     ELASHALLSGSGEVVLDERTITELEPDTAVAPAADTAELSFQLLAESIEAIERGDFRLV
                     SSPAMGLIAGGIFGRFAYLFEDGTALRDVMPPAADNVLRAQLAFQPAGGRQSNVMRVPT
                     VTERTLAIGVFDERGQDSVLGVDDVGILAEAGSLSLVSLRDQRRINALSPQMLDSINRT
                     PNVARLCREVTVAHTRPPRPWRWGDVDYLPYLPRVRYGRTVLAQARWRPSGELIEASAD
                     TSTWNTALDRWRERLRVPDTVQARHGDHSLELDLNAPLHRRLLADELRRKPELVITETA
                     RCGESDFDWLDGHANEIVVPLRAHRNESTVNRPARRAASWTPATARRSIPPGGEWVFAK
                     LYADSALHHEILRDHLPSFLDSVDSGVDRWFFMRYADPDEHVRIRFHGRPEVLNQSLLP
                     QLHSWTRVLHENGLIRKLALDSYEPEVNRYGGPEGIAAAEHAFRADSEAALAQLPLFDA
                     LNRDVPAEVLAAVNFVDLLRQTDPDWTSWYRRSDIQHYPGMHRHVSRAVQLTDALLAER
                     QTDSDRMVTTIRAACQHRASTLQAYARALRTRDEPTPDRHRSIVNSLLHMHHNRLLGGN
                     QDSEMRAHVVASRVAAAYKGRAKARQT"
     aSDomain        3352..3630
                     /aSDomain="Lanthipeptide_LanB_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing LanB protein in a class I
                     lanthipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_A37U_RS0135860_Lanthipeptide_LanB_RRE
                     .1"
                     /evalue="4.10E-16"
                     /identifier="RREFam001.1"
                     /label="A37U_RS0135860"
                     /locus_tag="A37U_RS0135860"
                     /protein_end="235"
                     /protein_start="142"
                     /score="50.5"
                     /tool="antismash"
                     /translation="VCANDLYFERAGRFVLPYVPLRDPTGQPAEEKSVRATPPARTAMT
                     VARAPIAFTELVDRLVEQYPQAAGTDRVEQLLMGLIETGLLCTELSPP"
     gene            5973..7265
                     /locus_tag="A37U_RS47065"
     CDS             5973..7265
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140: Lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497172.1"
                     /locus_tag="A37U_RS47065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lanthionine synthetase C family protein"
                     /protein_id="WP_020501486.1"
                     /sec_met_domain="LANC_like (E-value: 4.7e-53, bitscore:
                     169.2, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTASTGLADTVDSVIETLAERLADPARTCPCGSAENWRPALDSE
                     CGLALLYAELGHSDPDYRYIAHRYLGEANQALSESGNHALYDGAPALAFAASCAVQREG
                     EYATLFAGLDEPVFRVVRVVLAAEHERLKAGATAEHYANFDLISGLAGLGRYLLRREQD
                     SPEARSLLEDVCAYFGALIRPIKREGHTLPGWWSDRPLNPSTEDSSGHINLGMAHGIPG
                     PLALLATAWRSGVRVHGHAEAIETIMDFLITWSDEDEFGRYWPQALDLDEYTHRPPRLT
                     RSRQAWCYGTPGIARAVQLAGFALGRQDWNTLAREAVWDMFRLPTEQWRVQDTCLCHGW
                     AGLLHVVARIHADDPRCELSGELDWLAQRTAAPFDPSLPYGYQQVGGLRAELLRDDPGF
                     LTGAAGIALALHAYRTTPRPASGWDTALLLD"
     gene            complement(7448..7675)
                     /locus_tag="A37U_RS54560"
     CDS             complement(7448..7675)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /locus_tag="A37U_RS54560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_020501487.1"
                     /transl_table=11
                     /translation="MQEQLAARMVEQAREQGLELTGPDGLLKQLTKTVLESALNAELTE
                     HLGHEKHQDSPGREGTNVRNGSRSKTVVSD"
     gene            complement(7817..8311)
                     /locus_tag="A37U_RS0135875"
     CDS             complement(7817..8311)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /locus_tag="A37U_RS0135875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_169526994.1"
                     /transl_table=11
                     /translation="MLAEIKTLGYRGSMNLLVRYLNQGRADTDRPPISPRRLTGLILSH
                     SDNLTDQQHKLRDELTAACPEMIELSDAVSRFATLLKPRNGNETRLAEWITTVRSFNLP
                     YLHAFPRGLDKDHAAVTAGMTLPYSNGPTEGHVNRLKMLKRQMYGRANLDLLRKRVLLA
                     T"
     CDS             complement(9010..>9182)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676872.1"
                     /locus_tag="A37U_RS47075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /protein_id="WP_020501489.1"
                     /transl_table=11
                     /translation="LAVSSNLHPSGFDEIMPKTLATATVDRLLHHAHVVVTQGDSFRLS
                     QATSGEGVKPLR"
     gene            complement(9010..>9184)
                     /locus_tag="A37U_RS47075"
ORIGIN
        1 tgtcaccggc tgagccgacc tggcccatcg agccgcgaat ctggtggcgc cacagcgatt
       61 gttgcggctt gcgcgagcga aattgacctc ctcggtcgct gtgcagcgtg cactcggcga
      121 cggtgccgcc gcgtcgggcg acggcggatt ccagggcgtc gagggcgagc tgggcgctna
      181 tgcggtcagc gatagagtag ccgacgatcc ggttggacca cacgtccttg accgcgcaga
      241 ggtagacctt gccttcgttg ctcgggtgtt cggagatgtc tgtcaaccac aggcgattcg
      301 gcgcgtgggc ggtgaactcc cgacgcacca gatcatcgtg tgcgggcgcg ctgggccggg
      361 acttgcggcc gcgacgtttc ttcccgaaca cgcaccacca ctggttggtc gagcagatct
      421 tccacaccgt gcggtcggac acctggtgcc cagcagcggt gatctcgtcg cgcaacaggc
      481 ggtagccgaa ttcggggtcc tcgtggtggg cgtcgtagat cgcgttggcc agatacgcct
      541 cggccagctc ggtgtcggtg accggtcggg caagccaggc gtagtagtgc tggcggtgga
      601 gtttcagcac ccgacacgcg accgccaccg gcacccggat gcgggcaccg gcagcgacca
      661 actccttcac gagcgggtag agcctttccc ggcagattgc cctgcgacag gtacgccgcc
      721 gcccggcgca acacctcgtt ctcctgctcc aacaggcgat tacgacgttt gagttcgcgc
      781 agctcggcca ccgcatacgg tgccattcgg ctgcgggctg ggatgttctt gacctcgcat
      841 ctaccaatac tacgaggctc ataggtatta cctatcgcgt tatttgatcg gggtattgga
      901 tttccgcagc gcgtattgct gtgctatggg gacaaggtca aagggaacag ctgcgatgct
      961 gcgccgagtt ggtctttgag gggatggata tgaatcagat cgatttgcat gatcgctgtg
     1021 cggtcgtgac aggcggaggt agtggtctgg ggcgggccat tgccgaacga ctgctgtcct
     1081 agggcaccaa ggtgatgttg tgggaccgct cggagcgact gttgcaagaa gcgtgcaaca
     1141 gtatggtcgc cggcgagtcg gtgcgcacgg ccgttgtgga tgtgaccgac ttcgatgctg
     1201 tggtcgaggc tgcttcggac gcggagcgtg tgctcggccg ggtcgatgtg ctggttaaca
     1261 gtgctggtgt cacgtgtcca cccgtcccga tcgggcagta ccgcaccgag gactggcaac
     1321 gcattctgga tgggaatctt tcgggggtgt ttttctgttg tcgcgccgtg ctcccgggca
     1381 tgattgcgca gcagtacggg cgtgtggtga atgttgcgtc gatggcgggg aaagagggca
     1441 atccgcaaga gacggcgtat tcggcggcca aggctggtgt gatcgggttg accaaggcat
     1501 tgggtaagga agtcgcagca tcgggggtgt tggttaacgc ggtggcgcca gggattttcg
     1561 ctacgccgat gcgcacgagt gccgcgtcga gggagctggt tgggaagctg ttggatcgta
     1621 ccccaccctg gccctggccc gcccggcaag cttcccggac ggcaaccctg atatgcgccg
     1681 tcaaccgaac agtgcaccgc tggtaacgct ccaacagtcc aggcaaaggc ccgcgggagg
     1741 tttgccacga acaaccccaa gccgcacaac gcattcgccg catccgcagt cgcaccacga
     1801 cccgacgccc gtcgaccgga acgtcggcca cctgcctccg gacataatca tgcacccggc
     1861 ccgtcacgac gccacattcc gggcaggcca cccgcccggc ccgcgtcgac gcccgaaccg
     1921 cgatcacccc ggcgtcgtca tcaacaccct cgatgaccag acaagacaat tccgaaaaca
     1981 tcagcctcaa cagcctattt acatcacacg aaccccaccc tgatccaagg catcgtcatc
     2041 ccaacgaacc aacacgcact gtcaccaaca atgggccaga gtcactcacc ggacagtccc
     2101 tgtgttctct cactggactg agctcggctt ctcgtggcgg cgatcggcac cgccacgaga
     2161 agccgagcca tgctcaacgg cacgcctgtg cggcacttac ccgcaggtga cgccctacgc
     2221 gtaaccggta ttcatccatg acctctggcc gttgattgac tcagagatcg tttcacgatg
     2281 acgtcacctg ccgcgtcacg gagatcatga tggaacgaac tgtcaacagc cgggaaagcc
     2341 ggggcgggta aggccacact ctgagttgca ggtgccgggg gtcgccgttg gcttcattcg
     2401 cgattttgcg atctccccgt cggccggcac cgtcctgact tcgaggtcaa agatgtcgag
     2461 ttcgtcttgg cgttgtgcga tcggagcact catcggaatt cctttcgtac gcaggaaaca
     2521 gccccgtaag agacccgggg tagcgtcatt gtgtcagtac ttgcaagcgc gtcgccagga
     2581 ttttcgcgca ttctcgccac cggacgtggt acaaaatgga cctgtgtgac agctttcagc
     2641 aggccaagcg gattgacctc accgcggtca tctgttcacc aaccgccacc gaacacggtg
     2701 cgtccgacga tcgttactat ttctcgaacc ttcgactcgc gccatgctct tgcggcaccg
     2761 ggcagcgttg gctagtcttc cgtaaagctc ctacggtgac agcggtatgg tgatcctgca
     2821 ggcatcgtcg aggaagatgc tagggggtcg gtgactcagg ggtggctcag cactgcgatg
     2881 ggtgaatcgg gttcgtttcg cgttgacggc gccgtgtttc tgcgaatgcc ggtattgcca
     2941 caccaccggg cgtatgccct cgccgccgaa cgcgatccgg gccaaggaaa cgaacgcgat
     3001 gaactgattc gaattctacg caccgtggtc gccgatccac tggtacgcga ggctctcgag
     3061 gtgtccagcg attcgtgggc caccgggcta cgcaggttgg aagagggcga gcacgtccgt
     3121 acacgccagc tgcgccgcgc cacttacgcc gcatgcaggt acctgttgcg gatgaccacc
     3181 cgcccaactc ccttcgggct catggccggg gtcgcggtgg gcagcttcga tgacgaaccg
     3241 ctggtcgaac tcggcaaggc gcaccgcaag ggcgttcgcc ccgatggcga gtggctcacc
     3301 gccctgctca ccgagtggga gagccgcaca gaagtggtgc ggtgcctgcg ggtctgcgcg
     3361 aatgatctgt acttcgagcg agccgggcgg tttgtgcttc cgtacgtgcc cctgcgcgat
     3421 ccgaccggcc aacccgcgga ggaaaaatcc gtccgcgcca ccccgcctgc gcgaaccgcg
     3481 atgaccgtgg cacgagcacc aatcgcgttt accgaactgg tcgaccgact tgtcgagcag
     3541 tacccgcaag ccgccggtac cgatcgagtt gaacagctac tcatggggtt gatcgagaca
     3601 gggctgctgt gtaccgagct gtcgccaccg ccccactgca ccgatcccat tacgtacgtg
     3661 cgggctacct tggaacgtac gctggcgccg gagaccgccg gcttgggcaa tatcgccgca
     3721 cggttcgacg agtacgcacg agcaccgctg ggttccggcc gccaggcatg gggtgcggcc
     3781 actagcgaag cgcacgagtt acacagcggg gcgggctcgc cgatacaggt ggacctgcgc
     3841 atggacgcgc ggatcacctt gccgcacacc gtcgcgcgcg aggtggaatc ggctgcgtcg
     3901 gtattgctgc ggttgtcccc ggcgagcgga tcaccaccgc accttgcgaa ttactatgac
     3961 gagtttctgg atagatacgg tagcggcaac gccgtgcctg tcaaggaggt gctggaccct
     4021 gagcgtggca tcggcccgcc tgccgggtat cgactgccgg cgaacagtcg actcggcgag
     4081 cccacgcaag aacgggatgg gccgcgcaac cgtaagctgg cagagctggc atcccacgct
     4141 ttgctttccg gctcgggaga ggttgtcctc gacgaacgga cgatcaccga attggaaccg
     4201 gacacagccg tcgcgcccgc ggcggatacc gcagagttga gcttccagtt gctcgccgag
     4261 tcgatcgaag cgatcgaacg tggtgatttc cggctggtct cttccccagc gatggggctg
     4321 atcgcgggcg ggattttcgg ccgattcgcc tacctattcg aggacggcac cgcactgcgc
     4381 gacgtgatgc ccccggccgc cgacaacgtg ttgcgcgcgc aactcgcctt ccagccggca
     4441 ggcgggcgcc agtcgaacgt catgcgcgtg cccacggtaa ccgagcgcac gctggcgatc
     4501 ggagttttcg acgagcgtgg tcaggacagc gtactcggcg ttgacgatgt cgggatcctg
     4561 gccgaagcgg gcagtctatc cctggtgtcc ctacgcgatc agcggcgaat caacgcgttg
     4621 agcccgcaga tgttggattc gatcaaccgc acaccgaacg tggcaaggct gtgtcgcgag
     4681 gtcaccgtgg cccacacgcg ccccccgcgg ccgtggcgat ggggcgacgt ggactacctg
     4741 ccgtacctgc ctcgggttcg ctacggccgg acagtgttgg cgcaggcgcg atggcggcct
     4801 tcgggtgagc tgatcgaggc tagcgcggat acctcgacgt ggaacaccgc actggatcgc
     4861 tggcgcgagc ggctgcgggt acccgacacc gtgcaagcgc ggcacggcga ccacagcctg
     4921 gaactcgacc tgaacgcacc actgcaccgc cggctgctcg ccgatgagct tcggcgcaaa
     4981 cccgagcttg tcatcaccga aaccgcgcgg tgcggcgaat ctgatttcga ttggttggac
     5041 gggcatgcga acgagatcgt ggtgccatta cgcgcgcacc gtaacgaatc caccgtgaat
     5101 cgaccagccc gccgcgccgc gtcatggacg cctgcgaccg cacggcgcag cattccaccg
     5161 ggcggtgaat gggttttcgc caagctctac gctgactccg cgctgcatca cgagatcctc
     5221 cgtgaccact taccgtcatt tctggacagc gtggattccg gtgtggatcg atggttcttc
     5281 atgcgctacg cggatccgga cgagcacgtg cggatccgtt tccacggtcg gccagaggta
     5341 ctgaaccagt cgcttctgcc ccagctccac tcatggacgc gcgttctgca cgagaacggc
     5401 ctgatccgca aactcgcgct ggacagctac gagccggagg tcaaccgtta cggtgggccc
     5461 gaagggatcg cggcggcgga gcacgctttc cgcgcggaca gcgaggctgc ccttgctcag
     5521 ctaccattgt tcgatgccct caacagggat gtgcccgcgg aagttcttgc cgcggtcaac
     5581 ttcgtcgacc tgttgcgcca gaccgatccg gactggacgt cctggtatcg gcgatccgat
     5641 attcagcatt atccgggaat gcaccgccat gtcagccgcg ctgtgcagct gaccgacgcg
     5701 ctgctcgccg agcggcagac cgattcggac cgtatggtga cgacgatccg tgccgcgtgc
     5761 caacaccgcg cgtccacgct ccaggcgtac gcccgagcgc tgcgcacgcg cgatgagccc
     5821 actcccgacc gacaccggtc gatagtcaac agcctattgc atatgcacca caaccggctt
     5881 ctcggtggca accaagattc cgaaatgcgc gcacacgtag tggccagcag ggtcgccgcg
     5941 gcatacaagg gacgtgcgaa ggcgagacag acatgaccac cgcctcaacg ggtttggccg
     6001 acaccgtcga ctcggtcatc gagacattgg ccgagcggct ggctgatccg gcccgaacct
     6061 gcccctgcgg cagcgccgaa aactggcggc cagcgttgga tagcgagtgc ggcctggctt
     6121 tactctacgc ggagctcgga cacagcgacc ccgattaccg gtacatcgcg catcgctacc
     6181 tcggcgaagc gaaccaagca ctgtccgaat cgggcaacca cgccctctac gacggcgcgc
     6241 cggcactggc ctttgccgca agctgcgcgg tgcagcgcga aggcgaatac gcgacgttgt
     6301 ttgccggatt ggacgaaccg gtattccggg ttgtccgcgt ggtcctggcc gcagaacacg
     6361 agcggctgaa agccggcgcc accgccgaac attacgcgaa tttcgacctc atctccggac
     6421 tcgccggact gggccggtac ctgctgcggc gagagcagga cagcccagag gctcggtcac
     6481 tgttggagga cgtgtgcgcg tatttcgggg cgttgatacg ccccatcaaa cgcgaaggac
     6541 atacgttgcc cggatggtgg agcgatcgcc cccttaatcc gtctacagag gacagttcgg
     6601 gacacattaa tttggggatg gcacacggca ttccaggacc actcgcacta ctggccacag
     6661 cgtggcgatc aggtgtgcgc gtccatgggc acgccgaagc gatcgagacg atcatggatt
     6721 tcctcatcac atggtccgat gaggacgaat tcggaaggta ctggccacag gcactcgacc
     6781 ttgacgaata cacccaccgc cccccgcggc tgacccgttc gcgccaagcc tggtgttacg
     6841 gcactccagg gatagccagg gcggtccaac tcgccgggtt cgcgctgggc cgtcaggact
     6901 ggaacactct agcgcgcgaa gccgtatggg atatgttccg gctaccgact gagcagtggc
     6961 gggtccagga cacctgcctg tgccacggct gggctggcct gctgcatgtg gtcgcacgaa
     7021 tccacgccga cgatccgcga tgcgaactca gcggtgagct ggattggctc gcgcagcgga
     7081 ccgcagcgcc cttcgaccca agtctgccgt acggttatca acaggtcggc gggctgcgtg
     7141 ccgaactact gcgcgacgac ccgggctttc tcaccggcgc cgccggcatt gcgctggcat
     7201 tgcacgcgta ccgcaccacc ccacgaccgg cgagcggatg ggacaccgca ttgctgctgg
     7261 attagcagtc ggcggtaatg ccttacggcc atcgacctcg gctacgcacg cgtggcccga
     7321 actactcacc aagcgcttcg gcccttgcct cgaacaggat cagcagcacg acacgtggcc
     7381 gtccagctga ggtggacccg gtcggctgga cagaccgccg cagcctgtcc aaccgacagg
     7441 gtctacttca gtccgagacc acggtcttcg accgggaccc attgcgcaca ttcgtgccct
     7501 cacggcccgg gctgtcctgg tgtttctcgt gcccgaggtg ctcggtgagc tccgcgttca
     7561 acgccgactc cagcaccgtc ttggtcagct gcttgagcag cccatccggt ccagtcagct
     7621 ccaggccctg ctcacgggcc tgctccacca tccgcgcagc caactgttcc tgcacagacc
     7681 gcgcatcctt acccgaggaa gtgttcacaa catccagtgt catcactcat cgtgcccatc
     7741 ccgccggact caactccggc gtgtcgggcc gaaaacaccg cttctgaggc tctgtcgctg
     7801 atcttgtggt gtttgcttat gtggcaagga gtacgcgttt gcggagtagg tcgaggttgg
     7861 cccgtccgta catttgtcgt ttgagcattt tgaggcggtt gacgtggcct tcggtgggtc
     7921 cgttgctgta gggcagtgtc atgccggcgg tcacggcggc gtggtccttg tcgaggccgc
     7981 gagggaaggc gtgcaggtag ggaaggttga acgaacgaac ggtcgtgatc cattcggcga
     8041 gtcgtgtctc gttgccgtta cggggtttga gcagcgtggc gaatcggctg accgcgtcgc
     8101 tgagttcgat catttccggg caggcggcgg tcagctcgtc gcgcagcttg tgttgctggt
     8161 cggtgaggtt gtcgctgtgg ctgaggatca gtccggtcag tcgccgcggc gagatcggtg
     8221 gccgatcggt gtcggcgcgg ccctggttga ggtagcgcac gagcagattc atgctgccgc
     8281 gataaccgag cgtcttgatc tcggcgagca acgtcgtgac cgggaccgcg ggaacgtcgg
     8341 tccggcgctg gcgcaagtgt tcgcggtacg gatggaccaa ggtgggctgg tacgccggag
     8401 cgcgcaccag gcgttcgggc tggtcgatgc gggcgtagcg tttgacggtg ttcagcccga
     8461 gattgagcct gcgagcgata tcgagcagcc ctaccccttg tcgagtaggt cgtgaacatg
     8521 tcgccagcgt tcgcgggtgg tcgccgcccg tttgccctcc ggtatcggca cgttggcttt
     8581 ggcccagcag ccgctgtgtg tggcgacttc cttgagcgcg gcttcgccgg gcccctttcc
     8641 acagaggcac tggtgggccg ctggcgaggt gcccgcgaca tgcgggtcct tttccagcgg
     8701 cccccagccc gaacccggtg tgcggctttc accgcggcgg gctctccgat gactgctacg
     8761 caagtagcgg cgttggctat ccgatgtgaa tggcatcgtg gcaggagcgg cagacgacca
     8821 gagtcttgcg ccgtcgccgc gccattttcg ggctcttcgt actgatgtgt gggtagttgc
     8881 tgggccctgt gaggggtgtc aatggccagt ttgttgtccc cgttggcggc cagatgttgt
     8941 tcccgttggc ggccatttag ggctccccga cggcggccag atggttcccc gttggggtgg
     9001 tggatggtgt tagcgcaatg gtttcactcc ttcgcctgag gttgcttggg agagccggaa
     9061 gctgtcacct tgggtgacga cgacgtgggc gtggtgcagg agcctgtcga cggtggcggt
     9121 ggccagggtt tttggcatga tttcgtcgaa cccggagggg tgcaggttcg agctgacggc
     9181 gagt
//
