LOCUS       NZ_KB905381             9432 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Sciscionella marina DSM 45152 A37UDRAFT_scaffold_1.2, whole genome
            shotgun sequence.
ACCESSION   NZ_KB905381
VERSION     NZ_KB905381.1
KEYWORDS    WGS; GSC:MIGS:2.1; IMPROVED_HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Sciscionella marina DSM 45152
  ORGANISM  Sciscionella marina DSM 45152
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Sciscionella.
REFERENCE   1  (bases 1 to 910873)
  AUTHORS   Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K.,
            Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A.,
            Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2013) DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type                :: bacteria_archaea
            project_name                      :: Sciscionella marina DSM 45152
            collection_date                   :: SEP 2006
            lat_lon                           :: Missing
            depth                             :: 516 m
            alt_elev                          :: Missing
            country                           :: China
            num_replicons                     :: Missing
            ref_biomaterial                   :: DSM 45152
            biotic_relationship               :: Free living
            rel_to_oxygen                     :: Aerobe
            isol_growth_condt                 :: 19196758
            assembly :: Velvet v. 1.1.04; ALLPATHS v. r41043
            finishing_strategy                :: Missing
            environment                       :: Sand; Sediment; Marine; Aquatic
            trophic_level                     :: Missing
            sequencing_meth                   :: WGS
            GOLD Stamp ID                     :: Gi11892
            Current Finishing Status          :: Level 2: High-Quality Draft
            Type Strain                       :: Yes
            Funding Program                   :: DOE-CSP 2012
            Isolation Site :: Marine sand sediment at a depth of about 500 m,
            the northern South China Sea
            Cell Shape                        :: Rod-shaped
            Temperature Range                 :: Mesophile
            Temperature Optimum               :: 28 C
            pH                                :: 7
            Gram Staining                     :: Gram+
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Improved High-Quality Draft
            Current Finishing Status          :: Improved High-Quality Draft
            Assembly Method :: Velvet v. 1.1.04; ALLPATHS v. r41043
            Genome Coverage                   :: Unknown
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000379465.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 01:37:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,209
            CDSs (total)                      :: 8,146
            Genes (coding)                    :: 7,961
            CDSs (with protein)               :: 7,961
            Genes (RNA)                       :: 63
            rRNAs                             :: 4, 4, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 1 of 185
            Pseudo Genes (frameshifted)       :: 52 of 185
            Pseudo Genes (incomplete)         :: 155 of 185
            Pseudo Genes (internal stop)      :: 15 of 185
            Pseudo Genes (multiple problems)  :: 34 of 185
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:59:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 901441
            Orig. end                         :: 910873
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KB905381.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 405877
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Nikos  Kyrpides (nckyrpides@lbl.gov)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-PGF and JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            Full annotations are available from IMG.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..9432
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="aminopolycarboxylic-acid"
                     /region_number="4"
                     /rules="(AesA and AesB and AesC)"
                     /tool="antismash"
     cand_cluster    1..9432
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(AesA and AesB and AesC)"
                     /kind="single"
                     /product="aminopolycarboxylic-acid"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9432
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:8461](-)"
                     /cutoff="10000"
                     /detection_rule="(AesA and AesB and AesC)"
                     /neighbourhood="5000"
                     /product="aminopolycarboxylic-acid"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..8461)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(AesA and AesB and AesC)"
                     /neighbourhood="5000"
                     /product="aminopolycarboxylic-acid"
                     /protocluster_number="1"
     misc_feature    complement(52..65)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.41"
                     /tool="antismash"
     misc_feature    892..910
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: medium, score: 28.47"
                     /tool="antismash"
     misc_feature    complement(1232..1246)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.88"
                     /tool="antismash"
     gene            1428..1823
                     /locus_tag="A37U_RS0110250"
     CDS             1428..1823
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A37U_RS0110250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_020496547.1"
                     /transl_table=11
                     /translation="MRDKSFSFLVAPQANGVHVVNSGRDGSARLLGRLGLQLSAAVQRL
                     PDSATVGRAKLSCETGYRRCRCGFRYRVTGFAAGAQGRSGLRHALRGAGEALHPARTAT
                     GAGVGLPSPGSLQWSLRELVPKQRALR"
     gene            complement(1893..2378)
                     /locus_tag="A37U_RS43045"
     CDS             complement(1893..2378)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /locus_tag="A37U_RS43045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_020496548.1"
                     /transl_table=11
                     /translation="MKPEPGIGDGTSPPDTIDALGFQRTTSWLLAGAGKLVLRRWAGML
                     ARLELTSTQYKLLLTLDETGPLGQQRLAELVGIDPRNAVPIIETSVEQGLLIRQVDPAD
                     RRRRVLELSSKGLAAAAKLRASTADIEEELLGSLDPADRTALRRTLGALLDASDPHP"
     gene            2489..3454
                     /locus_tag="A37U_RS0110260"
     CDS             2489..3454
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (11-186). E-value: 3.4e-23.
                     Score: 74.2. Matches aSDomain:
                     nrpspksdomains_A37U_RS0110260_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642023.1"
                     /locus_tag="A37U_RS0110260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_020496549.1"
                     /sec_met_domain="ADH_N (E-value: 3e-06, bitscore: 22.8,
                     seeds: 66, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAVRFDHYGDVDVLDVREVDAPVATAGRVVVRIRAAAANPGDIY
                     VREGLAEQAWALRARLSGRATDAPRQSMTFPAGQGTDLAGEVVAVGDGVEHWRVGDEVL
                     GWSEERVGQAELAAVPAGHLVPKPADMSWEVAGSMYIAPMAALASVEAVSPAPGETVVV
                     SGAAGAVGVVAVQLARRSGAVVIGLTRQTNHDRMRRLGAIPVTYGDGQAARCRSAAGDR
                     IGAFIDTYGAGYVDLAHELGVPAKRINTIVDFHAAIDGRAGIVGTNDAGGATGLQRLVD
                     LVAAGELDVPVAGTYSLADVREAYRRVAEQRTGGKIVLIP"
     aSDomain        2522..3046
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A37U_RS0110260_PKS_ER.1"
                     /evalue="3.40E-23"
                     /label="A37U_RS0110260_PKS_ER.1"
                     /locus_tag="A37U_RS0110260"
                     /protein_end="186"
                     /protein_start="11"
                     /score="74.2"
                     /tool="antismash"
                     /translation="VDVLDVREVDAPVATAGRVVVRIRAAAANPGDIYVREGLAEQAWA
                     LRARLSGRATDAPRQSMTFPAGQGTDLAGEVVAVGDGVEHWRVGDEVLGWSEERVGQAE
                     LAAVPAGHLVPKPADMSWEVAGSMYIAPMAALASVEAVSPAPGETVVVSGAAGAVGVVA
                     VQLARRSGAVVI"
     CDS_motif       2960..3022
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A37U_RS0110260_0001"
                     /evalue="1.30E-04"
                     /label="PKSI-ER_m5"
                     /locus_tag="A37U_RS0110260"
                     /protein_end="178"
                     /protein_start="157"
                     /score="14.7"
                     /tool="antismash"
                     /translation="GETVVVSGAAGAVGVVAVQLA"
     gene            complement(3595..5004)
                     /locus_tag="A37U_RS0110265"
     CDS             complement(3595..5004)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501616.1"
                     /locus_tag="A37U_RS0110265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MATE family efflux transporter"
                     /protein_id="WP_020496550.1"
                     /transl_table=11
                     /translation="MNPRTGVRDYRPLIAVSAPIAGIQLAQVALTTTDLTMMGLMSVEA
                     IAAGGLAITLYNQLRTMCVGMVTAVGNLVASSAARGEDDRRSPGERLGGKAEEEVRQVV
                     RSAFLVATAMAVLGAGILIGLGYAMVWFGQSAHTLELARPAIVTLAPGLIPMLWLNVLR
                     QFAVGMRRPGSLLWVTLASIAVNAALDGAFIYGWMGMPRLGLAGIGLATTLVQFVNFFA
                     FAMIVRRDRQLRHLLSLRGWKADIRTVREIVRLGVPICLTYGSEAGITSVATVLMGTFG
                     PAMLAAHNIVNQLAYIAYQFNIGISQGSSILISRAASKNDPAAARTIAGRAMRLSAVLM
                     ALFAVFYLVAPSWALWLFLDHHDTPQIQAAAHTLLYLAIAHQFFKGAQNVNVGLLRGLG
                     NTKSGFHITLIGYWAIGVPAMLLAGYALDLGGPGIWIGLCLGFGATAVLLLRRFHRDLP
                     TATTPSVLSAA"
     gene            complement(5001..6095)
                     /locus_tag="A37U_RS0110270"
     CDS             complement(5001..6095)
                     /EC_number="2.5.1.-"
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /GO_process="GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     aminopolycarboxylic-acid: AesC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501617.1"
                     /locus_tag="A37U_RS0110270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cysteine synthase family protein"
                     /protein_id="WP_020496551.1"
                     /sec_met_domain="AesC (E-value: 1.8e-151, bitscore: 499.7,
                     seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="PALP (E-value: 1.2e-56, bitscore: 188.0,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTFLHSGRENIVSTVSELIGHTPLFHLGTAQSGSRLLLKLEQFNP
                     TGSAKVRMARQMLIHAENSGQLGPEGHIIEPTSGNTGLGIALAAIERGYRFTAVVDHHA
                     SKDKLRAMAAMGANLYYVGDPETPGPRTLERKQVANELAASVDGGYRPDQHNHPGNQEG
                     YQGLAHELLADIFGDLDYLISSVGTGGSLCGTARELRRLGSRVRTVGVEPVGSIIFGTS
                     GGRYWQTGGGNPEGFPLGSNIDYGLIDEGVQVGDVAAFATARVLARRTGLLIGGTAGGA
                     IYAALERLDTATPGSTIATLVCDAGEKYLDTIFDDDWMHARDLLDPTVEELVQQRLDQL
                     VKHVPAPRAHIGAPDTLEKVGSTG"
     gene            complement(6117..7490)
                     /locus_tag="A37U_RS0110275"
     CDS             complement(6117..7490)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     aminopolycarboxylic-acid: AesB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501618.1"
                     /locus_tag="A37U_RS0110275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Y4yA family PLP-dependent enzyme"
                     /protein_id="WP_240437525.1"
                     /sec_met_domain="AesB (E-value: 4.3e-181, bitscore: 597.6,
                     seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 2.8e-20, bitscore:
                     68.4, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELRFEQENVRAPAASLPALEPEWALRLRADQRLLTEIAEAVGGP
                     FHVLCPDRFVDNLTAFQTTLAESGVPGQVYFGKKANKSGCWLRACAEADAGVDVASVPE
                     LAHALRNGVRGEDIVVTGAAKTEELLWLAARQRCLIAVDAVDELERVIEKAHRTGTIRI
                     LLRVLPEHNPDSRFGLAETDLRHCLRRCAEQRQRVRMEGFSFHLNGYAVSPRAALADRL
                     IDECLAARELGLVADSISIGGGFAVNYLDPADWRYFERAHRSSWFHANRVFSHFYPYAQ
                     APVGADMLDEILASPTAAAGERLRTRFLETGTRLLLEPGRALLDGCGFSVFPVQGSKRR
                     EDYGIVTVSGLSMSVSEQWKASEYLPDPILWPEGEGQPINACIGGSSCLDYDMLTWRKV
                     PFTRLPKFGDLLIYPNTAGYQMDKNETEFHQLPLPDRIIVSERQGRWHWRIDKLPHIR"
     gene            complement(7481..8461)
                     /locus_tag="A37U_RS0110280"
     CDS             complement(7481..8461)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     aminopolycarboxylic-acid: AesA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158: ornithine cyclodeaminase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501619.1"
                     /locus_tag="A37U_RS0110280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_020496553.1"
                     /sec_met_domain="AesA (E-value: 7.7e-132, bitscore: 434.5,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEPQLHVFTRTDLADLELAPRDVIEAVRQGYLALAEGKSRCPTK
                     LMMPLPEAERDAVSYSMLGYDGANEYLGFKTSYRQGTDNPDKYYTTISLYDNSTGMPFA
                     LLDCERVGASRTPATTALIAQACAKPDAKSVLMIGTGVQGRNTLPYLQTALPGLERRML
                     YGTHPDGISSSLETFHRHFPDQEIELVSDVPQAVEESDIVVVASGRAAHPKVQTSRMKP
                     GGLMISVSSKGVDSSALRDADYAIATSAGQLGVTGSRFDSEDGKATIDAEFPEVLAGRV
                     PGRRADDDRVFVFSSGMIITDIPVAHVLARRAIAAGRGQRVALWS"
     misc_feature    complement(8552..8570)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.81"
                     /tool="antismash"
     misc_feature    complement(8790..8808)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 25.25"
                     /tool="antismash"
ORIGIN
        1 ctcggcccgg tactcggcgg gctggtgctt tcctcgctcg actggcggtg gatgttctgg
       61 atcaatctgc cgatcggcgc gctggccttc ctcggtgcgc tgcggctgat ccccgccgat
      121 gaccccggcg acgcggaccg cttcgatgtg ctcggtttcc tgttgctcgg cccgggattc
      181 gcgctgtcca tgctcgggct cgcccggctc ggcgaggaac ccggcacgct cggcgcgatc
      241 cttccgctgc tggcggggat cgcgctgctc gcgtgttatg tgccgcgcgc gttgcgcggg
      301 cggcggaaac cgatcctgaa tccacgactg ttcaccaatc gggggttcac cggggcggtc
      361 gcggtgatgc tgttcaccgg tgcggcgatg ttctccatca cctatctgtt cccgctgtac
      421 taccaacaac tgcgcggtct cggccagttc gcggccgggc tgctgatcgc gccgttcggc
      481 atcggttcgg cgatctcgat gccgcttgcc gggcggctcg ccgatcgggt cggggcgcgc
      541 ggcctcgccg gatcgggggc cgccgtgtcc gtcttcgtca tgctgggcct gagtttcgcg
      601 ggcgccgaca ccggtctcgg ctgggtcctg gccggactgt ttctcgtcgg cgccgccatg
      661 ggcctggtgg ccgcgccgac gatgggttcg ctgtatcgca gcctgccgga ggagcaggtc
      721 gcgcagggca gttccgcgct gtatatgctc aaccagttcg gtgcctcatt cggggtcgcc
      781 cttgtcatgc tcctgatgca gggcggtgcg ggctatccgg gtgcggcctg gtggatcgcc
      841 ggggcactgc tggccgtggt gctcgtcgcc aggctgctgc cccggttggc attaggcgag
      901 gctcacctac gttaaacctg gtcgaatggt ggcggcactg ctgaccgtgc tgctcggatg
      961 cggactgacg gcgtgcggat cgggcaccgg aaccgaggac gcgacagccg ccggtggcgg
     1021 tccgtttccc gtgacggtgc aaggaaaact cggagaggcg cagatcgtgc accggccgca
     1081 gcgggtggtg gcgctggacc ggacgagcgc ggacatcgcg gtgcccctcg gcgtgcggcc
     1141 ggtcgccatc gcctcggtgg ccggtgcccc gaacgggatc gaaccgtgga ccgcggcgcg
     1201 gctcggcggt tcggcaccgg agcggttctc catcaccaac ggtgatccga tcgagaagat
     1261 cgcctcgtgc cgtccggatc tgatcctcgc ggcgaaggac tacgacctca cggactcgta
     1321 cccgaaactg agcaggctca cccggtggtg cactacaccg atggcccgac cggctgatcg
     1381 ccgagaccaa ggaggcgatc ggcaaggcac gtggcgagct gtcctccttg cgggacaaga
     1441 gcttcagctt cctcgtcgcc ccgcaggcga acggggtgca cgtggtgaac tccggccggg
     1501 atgggagcgc gcggctgctc ggccggctcg ggctgcagct ctccgccgcg gtgcagcggc
     1561 tgccggactc ggcgaccgtg ggccgggcga agttgagctg cgagacgggg taccgccgat
     1621 gccgatgtgg ttttcgctac cgggtaaccg gattcgctgc aggcgctcaa ggacggtccg
     1681 gccttcggca cgctctccgc ggtgcgggcg aggcactaca tcccgctcga accgcaactg
     1741 gcgcaggcgt tggccttccc tcgccgggca gcctgcagtg gtcgctgcgc gagctcgtgc
     1801 cgaagcagcg cgcgctgcgc tgaggctcga ttcgcggatg ctgttcgggt gaggcgccgc
     1861 cggactgtcc gcgcccgccg cttcagcgat ggtcaggggt gcggatcgga tgcgtcgagc
     1921 agggcaccca gtgttcgccg cagggcggtg cgatcggccg gatcgagcga gcccagcagt
     1981 tcttcctcga tgtccgcggt cgaggcgcgc agcttcgcgg cggctgctag gcccttggag
     2041 gagagctcca gtacccgtcg gcgccggtcg gcggggtcga cctgacgaat cagcagtccc
     2101 tgttcgaccg aggtctcgat gatcggcacg gcgttgcgcg ggtcgatgcc gaccagttcg
     2161 gccagtcgct gctggccgag cggaccggtc tcgtcgagcg tcaacaggag cttgtactgc
     2221 gtcgaggtga gctcgagccg ggcgagcatg ccggcccagc gccgtaggac gagcttgcct
     2281 gccccggcga gtagccacga ggtggtgcgc tggaatccga gcgcatcgat cgtgtctggg
     2341 ggagaggtgc cgtcgccgat gcccggttcg ggtttcacga tacgcagtct agcggagccg
     2401 acatctccta tgatagacat gtgcaatgta ttaacatgag acatgtctaa ttgtggccgg
     2461 gagtcggcgt gatcagagga gttcgaggat gagagcagtt cgtttcgacc actacggcga
     2521 tgtcgatgtg ctcgatgtgc gcgaggtcga cgccccggtg gccaccgcag ggcgggtggt
     2581 ggtgaggatc cgggcagccg cggccaaccc gggagacatc tacgtacggg aagggctcgc
     2641 ggaacaagcg tgggctttgc gggcgcggtt gtccgggcgg gcgacggatg cgccccggca
     2701 gtcgatgacc ttccccgcag ggcagggcac ggacttggca ggggaggtcg tcgccgtcgg
     2761 tgacggggtc gagcactggc gggtcggcga tgaagtgctc ggctggtccg aggagcgagt
     2821 cggccaggcc gaactcgccg ccgtgccggc cggtcatctc gtccccaagc ccgccgatat
     2881 gagctgggag gtcgccggct cgatgtacat cgcgccgatg gccgcgctcg ccagcgtcga
     2941 ggcggtttcc cctgcgcccg gcgagaccgt tgtcgtgtcc ggcgccgccg gtgcggtcgg
     3001 agtcgtagcg gttcagctcg cccgtcgcag cggtgccgtc gtcatcggtc tgacccgcca
     3061 gaccaaccac gatcggatgc gccgccttgg cgccatcccg gtcacctatg gcgatggcca
     3121 ggcggccagg tgccggtcgg ccgccggcga tcggatcggg gcgttcatcg acacctacgg
     3181 cgccggctac gtcgacctcg cgcatgagtt gggtgttccc gcgaagcgca tcaacaccat
     3241 cgtggacttc cacgccgcga tcgatggtcg tgccggaatc gttggcacca acgacgccgg
     3301 cggggccacc gggctccagc gcctcgtcga tctcgtcgcc gcgggcgagc tcgacgtccc
     3361 tgtcgcggga acctactccc tcgccgatgt ccgcgaggcc taccgtcggg tcgccgaaca
     3421 gcgaaccggc ggcaaaatcg tcctgatccc gtgatcgtcg gtcggcagga acggccggtc
     3481 ggcaggacac tcgcttcccg gcccggcgaa cgccgacgag accgctgaac tcggcagctc
     3541 aggcaacgcc aggcagatcg ataccgatgc gtcaccgacg aaagggagcc gtggtcaggc
     3601 cgcgctgagc acggacggtg tggtggcggt ggggaggtct cggtggaagc ggcgcagcag
     3661 cagcacggcc gtggcgccga atccgaggca gagcccgatc cagattcccg gcccgccgag
     3721 gtcgagggcg tatccggcca gcagcatggc aggcacgccg atcgcccagt agccgatcag
     3781 ggtgatgtgg aaaccgctct tggtgttgcc gagccctcgt agcaggccga cgttgacgtt
     3841 ctgggcgccc ttgaagaact ggtgcgcgat cgcgaggtac agcagagtgt gcgcggccgc
     3901 ctggatctgc ggggtgtcgt ggtggtcgag gaacagccac aacgcccagg acggtgcgac
     3961 caggtagaaa acggcgaaca aggccatcag tacggcgctg aggcgcatcg cccgtccggc
     4021 gatcgtgcgc gccgccgccg ggtcgttctt gctcgcggcc cggctgatca gaatcgacga
     4081 gccttgggag attccgatgt tgaactggta ggcgatgtag gccagttggt tcacgatgtt
     4141 gtgcgccgcg agcatcgccg ggccgaaggt gcccatcagc acggtggcga ccgaggtgat
     4201 gcccgcttcg gagccgtagg tcagacagat cgggacaccg agccggacga tctcacgaac
     4261 cgtgcggata tcggctttcc agccccgcag ggacagcaga tgccgcagct gccggtcgcg
     4321 gcgcacgatc atggcgaagg cgaagaagtt gacgaactgg accagcgtcg tcgcaagtcc
     4381 gatcccggcc agccctagtc gcggcatgcc catccagccg tagatgaagg caccgtcgag
     4441 cgcggcgttc accgcgatgg aggcgagcgt gacccacagc aatgagccgg gtcgtcgcat
     4501 gccgacggcg aactggcgca ggacattgag ccagagcatc ggtatgagtc ccggcgcgag
     4561 cgtgacgatg gccggacgcg cgagttcgag ggtgtgcgcg ctctgcccga accagaccat
     4621 ggcataaccc aggccgatga gaatcccggc gccgaggacg gccatggcgg tggcgacgag
     4681 gaacgccgag cggacgacct ggcggacctc ttcttcggct ttcccgccga gtcgttcgcc
     4741 gggtgatctc cggtcgtcct cacctcgcgc agcactgctc gccacgagat ttccgaccgc
     4801 ggtcaccatg cccacacaca tggtgcgcag ctggttgtag agcgtgatcg cgagaccgcc
     4861 ggcggcaatg gcctcgacac tcatcagacc catcatcgtc agatcggtcg tggtgagggc
     4921 gacttgggcg agctggatcc cggcgatcgg cgcggacacc gcgatcaggg ggcggtagtc
     4981 ccgcactccg gtgcgcggat tcatccggtg gaacctactt tctccagggt gtccggggcg
     5041 ccgatgtggg cacgcggtgc cggtacatgc ttgaccagct ggtcgagccg ttgctgcacg
     5101 agctcctcga ctgtgggatc gagtagatcc cgggcatgca tccagtcatc gtcgaaaatg
     5161 gtatcgaggt atttctcccc ggcgtcgcag acgagggtcg cgatggtgct tcccggggtg
     5221 gcggtgtcga gccgttcgag tgcggcgtag atcgccccgc ccgctgtgcc cccgatcagc
     5281 agaccggttc ggcgggccag gacgcgcgcc gtggcgaacg ccgccacatc gccgacttgg
     5341 actccttcgt cgatcagtcc gtagtcgata ttgctgccga gtgggaatcc ctcggggttg
     5401 ccaccgccgg tctgccagta cctgccaccg ctcgtgccga agatgatcga cccgaccggc
     5461 tccacaccca ccgtgcggac ccgggaaccg agccggcgca gctcgcgtgc cgtgccgcag
     5521 agcgagcccc cggtgccgac ggagctgatc aggtagtcga ggtcgccgaa gatgtcggcg
     5581 agcagttcat gcgcgagccc ttgatatccc tcttggttgc cgggatggtt gtgctgatcc
     5641 ggccggtatc ccccgtcgac cgatgcggcg agttcgttgg cgacctgttt gcgctcgagc
     5701 gtccggggac cgggtgtctc cggatcgccc acgtagtaca ggttcgcgcc catcgccgcc
     5761 atggcacgga gtttgtcctt cgaagcatga tggtccacca cggcggtgaa gcgataacca
     5821 cgttcgatgg cggccaacgc gatgcccagg ccggtgtttc cggaggtcgg ttcgatgatg
     5881 tggccctcgg gacccagctg ccccgagttc tcggcgtgga tgagcatctg ccgcgccatc
     5941 cgcaccttgg ccgagccagt ggggttgaac tgttcgagct tcagcagcag ccgacttccc
     6001 gactgtgcgg ttcccaggtg gaaaagcggc gtgtgcccga tcagttccga aaccgtggag
     6061 acaatatttt cgcgaccgct gtgcaaaaag gtcatggatc ggctctttct cgtttgtcat
     6121 cgaatatgcg gtagcttgtc gatgcgccaa tgccatcggc cttgccgttc cgagacgata
     6181 attctgtccg gcagtggaag ttggtggaat tcggtttcgt tcttgtccat ctgatatccc
     6241 gccgtgttcg gatagatcag caggtcgccg aatttcggaa gccgtgtgaa cggcactttc
     6301 cgccaggtga gcatatcgta gtccagacaa ctcgatccgc cgatgcaggc gttaatcggc
     6361 tggccctcac cttccggcca cagtatcggg tcgggcaggt attcacttgc tttccattgt
     6421 tcggagacgc tcatgctcaa cccggaaacg gtcactattc cgtagtcttc ccggcgtttg
     6481 cttccctgta cggggaatac gctgaaccca cagccgtcga gcagtgcccg cccgggttcc
     6541 aggagcaacc gggttccggt ctcgaggaat ctcgtccgaa gccgctcacc ggccgcggcg
     6601 gtcggactcg cgaggatctc gtcgagcatg tccgcgccga ccggggcctg cgcatagggg
     6661 tagaagtggc tgaacacgcg gttggcgtgg aaccaggagc tccggtgcgc acgttcgaag
     6721 taccgccaat cggccgggtc gaggtagttg accgcaaagc ctccgccgat cgatatcgag
     6781 tcggcaacca ggcccagctc tcgggcggca aggcactcgt cgatcagccg gtcggcgagt
     6841 gccgcccgcg gcgagacggc ataaccgttg agatgaaagg agaaaccctc cattcggacc
     6901 cgctgtcgtt gctcggcaca ccggcgcagg cagtgccgga ggtcagtctc ggcgagcccg
     6961 aatcggctgt ccggattgtg ctcgggcaac acacgcaaca gaatcctgat cgtcccggtg
     7021 cggtgcgctt tctcgatcac ccgttccaac tcatcgacgg cgtccaccgc gatcaggcag
     7081 cgctgcctgg ccgccagcca gagcagttcc tcggtcttcg cggcaccggt gacaacgatg
     7141 tcctcgccgc gcacgccgtt gcgcagggcg tgcgccagct cgggcacact ggccacgtcc
     7201 acgcccgcgt ccgcctccgc acatgcgcga agccagcagc cggacttgtt ggccttcttg
     7261 ccgaaataaa cctgtcccgg gacgccggat tcggccagtg tggtctggaa tgcggtcagg
     7321 ttgtccacga accggtccgg gcacagtacg tggaatggac caccgaccgc ctcggcgatc
     7381 tccgtgagca gccgctgatc ggcccgcagg cgtagcgccc attccggctc cagtgcgggc
     7441 aaagacgcgg cgggggccct gacgttttcc tgttcgaacc tcaactccac agtgccaccc
     7501 gctgaccgcg accggccgcg atggcccgcc gcgccaggac atgcgccacc ggaatgtcgg
     7561 tgatgatcat gccactgctg aacacgaaga cccgatcgtc atcggcgcgg cgtcccggca
     7621 cgcgaccggc cagcacctcg ggaaattccg cgtcgatcgt ggctttgccg tcctcgctgt
     7681 cgaaacgcga accggtgacg cccagctgtc ccgcgctcgt cgctatcgca tagtcggcat
     7741 cgcgcagcgc ggaggagtcg acgcctttgc tcgataccga aatcatcagg ccgcccggct
     7801 tcatccggct cgtctgcacc ttcgggtgcg cggcgcgccc ggatgcgacc accacgatgt
     7861 ccgattcctc caccgcctgt gggacatccg agacgagttc gatctcttga tccgggaagt
     7921 gccggtgaaa ggtctcgagg ctcgaggaga tgccgtccgg atgcgtgccg tacagcatgc
     7981 gccgttcgag accgggcaac gcggtctgca gatacggcaa ggtgttccgt ccctggaccc
     8041 cggtgccgat catcaggacg gatttcgcat cgggtttcgc gcaggcctgg gcgatcagcg
     8101 cggtcgtagc gggcgtccgt gacgccccga cccgttcgca atccagcaaa gcgaacggca
     8161 tgccggtgga attgtcgtac aaactgattg tcgtgtaata cttgtccgga ttgtcggtcc
     8221 cttgccgata cgaggtcttg aatccgagat attcgttcgc accgtcatag ccgagcatcg
     8281 agtacgagac tgcgtcgcgc tcggcttccg gcagcggcat catcagcttc gtggggcacc
     8341 ggcttttccc ttcggccagg gcgagatatc cctgccgcac cgcttcgatc acgtctctcg
     8401 gcgcgagttc gaggtcggcc agatcggtcc tggtgaatac atgcaattgc ggttcggtca
     8461 tcaagcgcgc tccaaagtcg gaacaagttc tcgctatcgg tgacgtggcc ctatccgccg
     8521 agtcgccgtt cgcacacaat gagacacaaa attaagtaag gcaaagctaa gtgacaagcg
     8581 ccatagggtg gaatcgacga aggcgcgacc cgcttatccc cgagcgggcc gacacgctcc
     8641 gtatattcgc gatttgttgt ggacaatggc gaactttggc gcgccgcccc ggtacgtgcg
     8701 taccgaatcg aggccgtacc taatcgggtg ggatgcgatc gaagtaacgg agtcggcgcg
     8761 gggcttgcgt gaccgtggcc gcatgtggat aaggttagcc atgcctaata tcagggactt
     8821 gcgtgcggcg gcatggcgcg tttctcggcg gcagcgtgcc gtcgtggctc tcgcgatggt
     8881 gcttctgctc gccgcgtgcg ggagcccgcc gggtgcgcag tcatccgatg ccgcgaagcg
     8941 cagtgtcacc gatgtactcg gccgccgggt cgaggtaccg gtgcccgcca agcgcatnct
     9001 gctcgatggc gcgcggatgc tttacaccac tgccgtgctg aacaagcgga acccacttgc
     9061 cggaattgtc ggattgccca acgatctgga gcagaacgac ccggactccc tgcaacagta
     9121 cgagaagaag ttccccggaa tcgacaagat ccagcggacc ggacaggttt atgacggatc
     9181 tttctcggtg gagaaggcca ttcagctgca cccggatgtc ttcgtgctga gtgcggcgaa
     9241 cttcaaggnc gcacaagacg ccggaatcgt cgacaagttg caacgagtgg ggatccccac
     9301 ggtcgtggtg gactacttcg agaaaccatt gcagaacact gtgccgagtg tccggctgat
     9361 gggccagtta ctcggccgcg acccgcaggc cgaacagtac gttcagtact accaagcggc
     9421 gatcgatcgg gt
//
